CN104388508A - Method for splitting insect body by utilizing insect virus to extract insect peptide - Google Patents
Method for splitting insect body by utilizing insect virus to extract insect peptide Download PDFInfo
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- CN104388508A CN104388508A CN201410573890.5A CN201410573890A CN104388508A CN 104388508 A CN104388508 A CN 104388508A CN 201410573890 A CN201410573890 A CN 201410573890A CN 104388508 A CN104388508 A CN 104388508A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention discloses a method for splitting an insect body by utilizing an insect virus to extract insect peptide. A living insect body is fed with the corresponding insect virus; the insect virus is greatly duplicated in the insect body, and a great number of proteolytic enzymes are generated; the structural protein of the insect body can be hydrolyzed into water-soluble peptide by the proteolytic enzymes, so that the insect body is hydrated; after an insect dies from hydration, a liquid mixture A containing an insect skin which is not hydrolyzed by the proteolytic enzymes, the insect virus and the water-soluble peptide is obtained; the insect skin in the liquid mixture A is filtered by a screen, and a liquid mixture B is obtained; the liquid mixture B is subjected to centrifugal treatment; a water-soluble peptide solution positioned on an upper centrifugal layer is separated. The water-soluble peptide solution which is obtained through the method and comes from the insect body is rich in the water-soluble peptide, and has a remarkable effect when serving as a plant foliar fertilizer; in addition, the process of the method is simple, the conditions are mild, and the method is economical, environmentally-friendly and efficient.
Description
Technical field
The present invention relates to polypeptide and extract field, specifically a kind of method utilizing insect viruses cracking insect body to extract insect polypeptide.
Background technology
Multiple amino acids, peptide and protein is rich in insect body.Experiment finds, the nutrition that the growth of polypeptide to plant of insect body is useful and growth promoting function.How efficient, gentle active polypeptide in extraction insect body be a significant problem.
The extracting method of common insect body polypeptide is the means with strong acid and strong base, such as, use the vitriol oil and concentrated sodium hydroxide under the high temperature conditions, is water miscible polypeptide, then collects these hydrolyzed peptide products in hydrolysis insect body.
The condition of this conventional means is violent, and in insect, a lot of highly active polypeptide cannot tolerate such exacting terms, under the hot conditions of strong acid and strong base, can lose activity.Meanwhile, this method contaminate environment, energy consumption is very large.So it is more gentle to find one, and more economic method is very important.
Summary of the invention
The object of the present invention is to provide a kind of mild condition, and economy, environmental protection, utilize insect viruses cracking insect body to extract the method for insect polypeptide efficiently.
For achieving the above object, the invention provides following technical scheme:
Utilize insect viruses cracking insect body to extract a method for insect polypeptide, comprise the following steps:
Step one, to the corresponding insect viruses of insect live body feeding; Insect viruses are massive duplication in insect body, and produces a large amount of proteolytic ferments; The structural protein of insect body can be hydrolyzed into water-soluble polypeptide by proteolytic ferment, thus by insect body aquation, insectization water and after death, obtains containing not by the worm skin of proteolytic enzymes hydrolize, insect viruses, and the liquid mixture A of water-soluble polypeptide;
Step 2, by the worm skin screen cloth elimination in liquid mixture A, obtain the liquid mixture B containing insect viruses and water-soluble polypeptide;
Step 3, carry out centrifugal treating to liquid mixture B, because insect viruses are protein encapsulation body, proportion is greater than water-soluble polypeptide, and what be therefore positioned at centrifugal lower floor is insect viruses, and what be positioned at centrifugal upper strata is water-soluble polypeptide solution; Water-soluble polypeptide solution is separated, namely obtains the water-soluble polypeptide solution deriving from insect body.
As the further scheme of the present invention: the water-soluble polypeptide solution deriving from insect body is directly used as plant-foliage fertilizer.
As the further scheme of the present invention: the water-soluble polypeptide solution deriving from insect body is used as plant-foliage fertilizer together with plant nutrient ingredient and/or plant-growth regulator.
As the present invention's further scheme:
Principle of the present invention is: insect viruses are divided into baculovirus and granulosis virus(GV) by its form; Insect viruses are single-minded pathogenic micro-organisms parasitized on insect; Insect infects virus by eating the virulent food of band; Virus carries out copying propagation in insect body, and produce viral distinctive proteolytic ferment, the method makes full use of the biological characteristic of insect viruses, utilize these proteolytic ferments the structural protein of insect body can be hydrolyzed into water-soluble polypeptide, thus by insect body aquation, insect viruses are discharged simultaneously, in the insect body liquid of aquation, be rich in water-soluble insect polypeptide and insect viruses.
Compared with prior art, the invention has the beneficial effects as follows: the water-soluble polypeptide solution of what the method obtained derive from insect body is rich in water-soluble polypeptide, as the Be very effective of plant-foliage fertilizer; In addition, the technique of present method is simple, mild condition, and economy, environmental protection, efficient.
Embodiment
Be described in more detail below in conjunction with the technical scheme of embodiment to this patent.
Embodiment 1
Get bollworm 300, culturing feed evenly sprays heliothis armigera nuclear polyhedrosis virus; Bollworm ate virus after 7 days, and polypide deliquescing starts aquation; Now collect bollworm; After the thorough aquation of polypide, hydrated liquid mixture is crossed 150 eye mesh screens, except debug skin and other impurity; Filtrate is carried out centrifugal; By centrifugal lower floor's virus purification out, supernatant liquid is exactly the water-soluble polypeptide solution of source insect body.
The water-soluble polypeptide solution of this source insect body is watered 100 times, directly to plant-spraying, arrange clear water control group, experiment proves that plant-growth is obviously better than clear water control group, and therefore the water-soluble polypeptide solution of source insect body has significant growth-promoting effect to plant.
Embodiment 2
Get Spodoptera litura larvae 300, culturing feed evenly sprays heliothis armigera nuclear polyhedrosis virus; Spodoptera litura larvae ate virus after 7 days, and polypide deliquescing starts aquation; Now receive the water-soluble polypeptide solution collection Spodoptera litura larvae of source insect body; After the thorough aquation of polypide, hydrated liquid mixture is crossed 150 eye mesh screens, except debug skin and other impurity; Filtrate is carried out centrifugal; By centrifugal lower floor's virus purification out, supernatant liquid is exactly the water-soluble polypeptide solution of source insect body.
Use this polypeptide solution 100 milliliters, add potassium primary phosphate 25 grams, 100 times can be watered, directly to plant-spraying.
By the water-soluble polypeptide solution 100 milliliters of this source insect body, add potassium primary phosphate 25 grams, be watered 100 times again, directly to plant-spraying, potassium dihydrogen phosphate control group is set, experiment proves that plant-growth is obviously better than potassium dihydrogen phosphate control group, and therefore the water-soluble polypeptide solution of source insect body has significant growth-promoting effect to plant.
Embodiment 3
Get silkworm larva 300, culturing feed evenly sprays Bombyx mori nuclear polyhydrosis virus; Silkworm ate virus after 7 days, and polypide deliquescing starts aquation; Now collect silkworm; After the thorough aquation of polypide, hydrated liquid mixture is crossed 150 eye mesh screens, except debug skin and other impurity; Filtrate is carried out centrifugal; By centrifugal lower floor's virus purification out, supernatant liquid is exactly the water-soluble polypeptide solution of source insect body.
Use this polypeptide solution 100 milliliters, 100 times can be watered, directly to plant-spraying.
By the water-soluble polypeptide solution 100 milliliters of this source insect body, add 100 grams, urea, be watered 100 times again, directly to plant-spraying, urea soln control group is set, experiment proves that plant-growth is obviously better than urea soln control group, and therefore the water-soluble polypeptide solution of source insect body has significant growth-promoting effect to plant.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, in the ken that one skilled in the relevant art possesses, can also makes a variety of changes under the prerequisite not departing from this patent aim.
Claims (3)
1. utilize insect viruses cracking insect body to extract a method for insect polypeptide, it is characterized in that,
Step one, to the corresponding insect viruses of insect live body feeding; Insect viruses are massive duplication in insect body, and produces a large amount of proteolytic ferments; The structural protein of insect body can be hydrolyzed into water-soluble polypeptide by proteolytic ferment, thus by insect body aquation, insectization water and after death, obtains containing not by the worm skin of proteolytic enzymes hydrolize, insect viruses, and the liquid mixture A of water-soluble polypeptide;
Step 2, by the worm skin screen cloth elimination in liquid mixture A, obtain the liquid mixture B containing insect viruses and water-soluble polypeptide;
Step 3, carry out centrifugal treating to liquid mixture B, because insect viruses are protein encapsulation body, proportion is greater than water-soluble polypeptide, and what be therefore positioned at centrifugal lower floor is insect viruses, and what be positioned at centrifugal upper strata is water-soluble polypeptide solution; Water-soluble polypeptide solution is separated, namely obtains the water-soluble polypeptide solution deriving from insect body.
2. the method utilizing insect viruses cracking insect body to extract insect polypeptide according to claim 1, is characterized in that, the water-soluble polypeptide solution deriving from insect body is directly used as plant-foliage fertilizer.
3. the method utilizing insect viruses cracking insect body to extract insect polypeptide according to claim 1, it is characterized in that, the water-soluble polypeptide solution deriving from insect body is used as plant-foliage fertilizer together with plant nutrient ingredient and/or plant-growth regulator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410573890.5A CN104388508A (en) | 2014-10-24 | 2014-10-24 | Method for splitting insect body by utilizing insect virus to extract insect peptide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410573890.5A CN104388508A (en) | 2014-10-24 | 2014-10-24 | Method for splitting insect body by utilizing insect virus to extract insect peptide |
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| CN104388508A true CN104388508A (en) | 2015-03-04 |
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| CN201410573890.5A Pending CN104388508A (en) | 2014-10-24 | 2014-10-24 | Method for splitting insect body by utilizing insect virus to extract insect peptide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017124588A1 (en) * | 2016-01-20 | 2017-07-27 | 中国科学院武汉物理与数学研究所 | Method for preparing recombinant adeno-associated virus |
| CN113072613A (en) * | 2021-04-06 | 2021-07-06 | 孙钥 | Insect virus schizolysis polypeptide extraction element |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101519442A (en) * | 2009-03-31 | 2009-09-02 | 安徽省农业科学院蚕桑研究所 | Method for inducing silkworms to produce antibacterial peptides |
| CN102584452A (en) * | 2012-02-22 | 2012-07-18 | 湛江师范学院 | Method for preparing biological antibacterial organic fertilizer by using immune induced biological technology |
| CN103708974A (en) * | 2012-10-08 | 2014-04-09 | 江西省新龙生物科技有限公司 | Process for producing virus biological insect-proofing organic fertilizer |
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2014
- 2014-10-24 CN CN201410573890.5A patent/CN104388508A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101519442A (en) * | 2009-03-31 | 2009-09-02 | 安徽省农业科学院蚕桑研究所 | Method for inducing silkworms to produce antibacterial peptides |
| CN102584452A (en) * | 2012-02-22 | 2012-07-18 | 湛江师范学院 | Method for preparing biological antibacterial organic fertilizer by using immune induced biological technology |
| CN103708974A (en) * | 2012-10-08 | 2014-04-09 | 江西省新龙生物科技有限公司 | Process for producing virus biological insect-proofing organic fertilizer |
Non-Patent Citations (3)
| Title |
|---|
| 刘思源等: "昆虫抗菌肽在农业上的应用潜力", 《天津农业科学》 * |
| 秦启联等: "昆虫病毒生物杀虫剂产业化及其展望", 《中国生物防治学报》 * |
| 赵东红等: "昆虫抗菌肽的功能、作用机理与分子生物学研究最新进展", 《生物工程进展》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017124588A1 (en) * | 2016-01-20 | 2017-07-27 | 中国科学院武汉物理与数学研究所 | Method for preparing recombinant adeno-associated virus |
| US10829784B2 (en) | 2016-01-20 | 2020-11-10 | Wuhan Institute Of Physics And Mathematics. Chinese Academy Of Sciences | Method for preparing recombinant adeno-associated virus |
| CN113072613A (en) * | 2021-04-06 | 2021-07-06 | 孙钥 | Insect virus schizolysis polypeptide extraction element |
| CN113072613B (en) * | 2021-04-06 | 2023-05-26 | 海南建邦制药科技有限公司 | Insect virus schizolysis polypeptide extraction element |
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