CN104388364A - Fermentation medium of seawater photosynthetic bacteria - Google Patents
Fermentation medium of seawater photosynthetic bacteria Download PDFInfo
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- CN104388364A CN104388364A CN201410761186.2A CN201410761186A CN104388364A CN 104388364 A CN104388364 A CN 104388364A CN 201410761186 A CN201410761186 A CN 201410761186A CN 104388364 A CN104388364 A CN 104388364A
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- Prior art keywords
- seawater
- photosynthetic bacteria
- fermentation medium
- peptone
- ammonium chloride
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a fermentation medium of seawater photosynthetic bacteria. The fermentation medium of the seawater photosynthetic bacteria is prepared by 0.2 g/L of ammonium chloride, 0.2 g/L of yeast extract powder, 0.4 g/L of peptone, 3.0 g/L of anhydrous sodium acetate, 0.02 g/L of iron phosphate and seawater, the PH ranges from 7.0-7.2, and the fermentation temperature ranges from 28 DEG C to 32 DEG C. The growth and reproduction speed can be increased, and the cultivation period can be shorted.
Description
Technical field
The present invention relates to field of microbial fermentation, particularly relate to the fermention medium of a kind of seawater photosynthetic bacterium.
Background technology
Main bait microorganism in current China sea cucumber culture pond is marine yeast and photosynthetic bacterium.The domestic kinds of culture medium to photosynthetic bacterium is many, complicated component.Culture medium prescription is generally ammonium chloride 1g, dipotassium hydrogen phosphate 0.5g, magnesium chloride 0.2g, sodium bicarbonate 5.0g, PH=7.0; Or adding a small amount of peptone and Sulfothiorine in the medium, this kind of method culture cycle is long, and substratum composition is complicated, and fermentation costs is higher.Substratum of the present invention is intended to solve the problem.
Summary of the invention
The present invention improves current marine photosynthetic bacteria used growth and breeding speed, provides a kind of marine photosynthetic bacteria used fermention medium.
For achieving the above object, the technical scheme of employing is in the present invention, the fermention medium of a kind of seawater photosynthetic bacterium, and consist of ammonium chloride, peptone, yeast leaching powder, anhydrous sodium acetate, tertiary iron phosphate, its special character is:
Ammonium chloride 0.2g/L;
Yeast leaching powder 0.2g/L;
Peptone 0.4g/L;
Anhydrous sodium acetate 3.0g/L;
Tertiary iron phosphate 0.02g/L.
Described substratum regulates PH7.0 ~ 7.2, Applicable temperature 28 ~ 32 DEG C.
According to above technical scheme, compared with prior art, seawater Fermentation by Photosynthetic Bacteria substratum disclosed by the invention has following advantage.
1, substratum of the present invention is applicable to seawater photosynthetic bacterium.
2, substratum of the present invention can shorten the culture cycle of photosynthetic bacterium, and improves pigment production.
3, this kind of substratum composition is simple, and have no side effect, configuration is convenient, cheap.
Embodiment
Specific embodiment of the invention effect will be described below.
Scheme 1: ammonium chloride 1.0g/L, potassium primary phosphate 0.6g/L, bitter salt 0.2g/L, sodium acetate 3.5g/L, dipotassium hydrogen phosphate 0.4g/L, calcium chloride 0.045g/L, yeast extract paste 0.1g/L, peptone 0.1g/L, PH7.2.
Scheme 2: ammonium chloride 0.2g/L, yeast leaching powder 0.2g/L, peptone 0.4g/L, anhydrous sodium acetate 3.0g/L, tertiary iron phosphate 0.02g/L, PH7.2.
Two schemes all inoculate 20%, 28 DEG C of cultivations, and record 660nmOD value.
After four days, scheme 1:OD 0.703, scheme 2 OD 1.132.
After six days, scheme 1:OD 0.909, scheme 2 OD 1.635.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are enable to repeat to implement and improve further.To be apparent for those skilled in the art to the multiple amendment of embodiment, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (2)
1. a fermention medium for seawater photosynthetic bacterium, its formula is that every 1000ml seawater contains: ammonium chloride 0.2g, yeast leaching powder 0.2g, peptone 0.4g, anhydrous sodium acetate 3.0g, tertiary iron phosphate 0.02g.
2. the fermention medium of a kind of seawater photosynthetic bacterium according to claim 1, described substratum fermentation Applicable temperature is 28 ~ 32 DEG C, PH7.0 ~ 7.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410761186.2A CN104388364A (en) | 2014-12-13 | 2014-12-13 | Fermentation medium of seawater photosynthetic bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410761186.2A CN104388364A (en) | 2014-12-13 | 2014-12-13 | Fermentation medium of seawater photosynthetic bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104388364A true CN104388364A (en) | 2015-03-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410761186.2A Pending CN104388364A (en) | 2014-12-13 | 2014-12-13 | Fermentation medium of seawater photosynthetic bacteria |
Country Status (1)
| Country | Link |
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| CN (1) | CN104388364A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830715A (en) * | 2015-04-15 | 2015-08-12 | 中国海洋大学 | Marine photosynthetic bacteria scale-production method |
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
-
2014
- 2014-12-13 CN CN201410761186.2A patent/CN104388364A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830715A (en) * | 2015-04-15 | 2015-08-12 | 中国海洋大学 | Marine photosynthetic bacteria scale-production method |
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN108220203B (en) * | 2018-03-06 | 2021-03-02 | 上海海洋大学 | Fermentation medium of rhodobacter sphaeroides and application of fermentation medium in fermentation production of rhodobacter sphaeroides |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150304 |
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| WD01 | Invention patent application deemed withdrawn after publication |