CN103881938A - Spectrum photosynthetic bacteria medium formula - Google Patents
Spectrum photosynthetic bacteria medium formula Download PDFInfo
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- CN103881938A CN103881938A CN201210556700.XA CN201210556700A CN103881938A CN 103881938 A CN103881938 A CN 103881938A CN 201210556700 A CN201210556700 A CN 201210556700A CN 103881938 A CN103881938 A CN 103881938A
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- photosynthetic bacteria
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- medium
- medium formula
- peptone
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a spectrum photosynthetic bacteria medium formula, which comprises the following ingredients: peptone, extract yeast, sodium acetate, sodium bicarbonate, ammonium chloride, magnesium chloride and dipotassium hydrogen phosphate. According to the invention, pH value of the medium does not increase by following with prolongation of the culture time, and always keeps between 5.5-7.5, photosynthetic bacteria growth is not inhibited, by employing the organic mediums with low concentration, such as peptone, extract yeast and NPK salt, so that the pure and bright color of the cultured photosynthetic bacteria can be endured, shallowing, greening and darkening are not generated; low cost can be guaranteed by employing nitrogen source and carbon source with low concentration, so that the medium formula of the invention is suitable for large-scale industrial production; and the photosynthetic bacteria cultured by the medium have the advantages of fast growth speed and high bacteria liquid concentration.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to microbial culture based formulas.
Background technology
Photosynthetic bacterium (Pho to syn thetic bac teria, PSB) is to utilize luminous energy and carbonic acid gas to maintain coloured bacterium of autotrophy life.On the earth, to occur the earliest, occurring in nature ubiquity, there are the prokaryotic organism of original luminous energy synthetic system, under anaerobic condition, do not put oxygen photosynthesis, do not form gemma ability, Gram-negative bacteria, be a class using light as the energy, can under anaerobism illumination or aerobic dark condition, utilize organism, sulfide, ammonia of occurring in nature etc. to carry out photosynthetic microorganism as the hydrogen donor carbon source of holding concurrently.
Photosynthetic bacterium is amphimicrobian, and the different photosynthetic bacterium floras required nutritive substance difference to some extent of growing, and this just makes the culture medium prescription of different photosynthetic bacterium floras change thereupon, cannot treat different things as the same.The formula ubiquity of existing photosynthetic bacteria culture medium suppresses photosynthetic bacterium growth, and speed is slower, and the photosynthetic bacterium color of turning out is impure, also can turn green even blackening, and cost is higher, is not suitable for the problem that large-scale industrialization is produced.
Summary of the invention
The above-mentioned defect existing in order to overcome prior art field, the object of the invention is to, a kind of photosynthetic bacteria culture medium formula of spectrum is provided, the formula ubiquity that solves the photosynthetic bacteria culture medium of prior art suppresses photosynthetic bacterium growth, speed is slower, and the photosynthetic bacterium color of turning out is impure, also can turn green even blackening, cost is higher, is not suitable for the problem that large-scale industrialization is produced.
The photosynthetic bacteria culture medium formula of spectrum provided by the invention, its components in mass portion proportioning is: 0.45-0.55 part of peptone, 0.45-0.55 part of yeast extract paste, 0.9-1.1 parts of sodium acetates, 0.9-1.1 parts of sodium bicarbonates, 0.9-1.1 parts of ammonium chlorides, 0.9-1.1 parts, magnesium chloride, 0.9-1.1 parts of dipotassium hydrogen phosphates.
The photosynthetic bacteria culture medium formula of spectrum provided by the invention, its beneficial effect is, in the present invention, Medium's PH Value does not raise along with the prolongation of incubation time, remains between 5.5-7.5, can not suppress the growth of photosynthetic bacterium; Adopt organic substratum such as peptone, yeast extract paste and the nitrogen phosphorus sylvite of lower concentration to guarantee that the photosynthetic bacterium color of turning out is pure, beautiful, can be not thin out, turn green, blackening etc.; Adopt nitrogenous source and the carbon source of lower concentration to guarantee low cost, more can be suitable for large-scale industrialization and produce; The photosynthetic bacterium growth speed of the culture medium culturing in the present invention is fast, and bacterial concentration is high.
Embodiment
Below in conjunction with an embodiment, the photosynthetic bacteria culture medium formula of spectrum provided by the invention is described in detail.
Embodiment
The photosynthetic bacteria culture medium formula of the spectrum of the present embodiment, its components in mass portion proportioning is: 0.5 part of peptone, 0.5 part of yeast extract paste, 1 part of sodium acetate, 1 part of sodium bicarbonate, 1 part of ammonium chloride, 1 part, magnesium chloride, 1 part of dipotassium hydrogen phosphate.
Described raw material is added to 1000 parts of distilled water by said ratio, be configured to the photosynthetic bacteria culture medium of spectrum, cultivate through dull and stereotyped, test tube is cultivated, and the steps such as triangular flask cultivation are turned out the good photosynthetic bacterium of quality.
Claims (1)
1. the photosynthetic bacteria culture medium formula of a spectrum, it is characterized in that: its components in mass portion proportioning is: 0.45-0.55 part of peptone, 0.45-0.55 part of yeast extract paste, 0.9-1.1 parts of sodium acetates, 0.9-1.1 parts of sodium bicarbonates, 0.9-1.1 parts of ammonium chlorides, 0.9-1.1 parts, magnesium chloride, 0.9-1.1 parts of dipotassium hydrogen phosphates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210556700.XA CN103881938A (en) | 2012-12-20 | 2012-12-20 | Spectrum photosynthetic bacteria medium formula |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210556700.XA CN103881938A (en) | 2012-12-20 | 2012-12-20 | Spectrum photosynthetic bacteria medium formula |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103881938A true CN103881938A (en) | 2014-06-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201210556700.XA Pending CN103881938A (en) | 2012-12-20 | 2012-12-20 | Spectrum photosynthetic bacteria medium formula |
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| CN (1) | CN103881938A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004162A (en) * | 2017-12-04 | 2018-05-08 | 连云港巨森农业科技发展有限公司 | A kind of photosynthetic bacteria culture medium formula |
| CN111019867A (en) * | 2019-12-30 | 2020-04-17 | 沧州市方元生物工程有限公司 | Culture medium for cultivating bacteria for treating river channel |
-
2012
- 2012-12-20 CN CN201210556700.XA patent/CN103881938A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004162A (en) * | 2017-12-04 | 2018-05-08 | 连云港巨森农业科技发展有限公司 | A kind of photosynthetic bacteria culture medium formula |
| CN111019867A (en) * | 2019-12-30 | 2020-04-17 | 沧州市方元生物工程有限公司 | Culture medium for cultivating bacteria for treating river channel |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140625 |