CN104382923A - Lincomycin hydrochloride breast injectant for dairy cow and preparation method thereof - Google Patents
Lincomycin hydrochloride breast injectant for dairy cow and preparation method thereof Download PDFInfo
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- A61K31/00—Medicinal preparations containing organic active ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
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Abstract
The invention discloses a lincomycin hydrochloride breast injectant for a dairy cow and a preparation method thereof. The lincomycin hydrochloride breast injectant for the dairy cow is composed of lincomycin hydrochloride, a suspending agent, a surface active agent, an antioxidant, a preservative and soybean oil for injection, wherein the lincomycin hydrochloride is taken as an active ingredient; under the condition of the antibacterial effect of the medicine is guaranteed, the surface active agent and the suspending agent are added for realizing a synergistic effect, so that pharmaceutical ingredients are uniformly dispersed, the beneficial effects of high consistency, good stability and biocompatibility, long acting time and obvious sustained release effect are achieved. The prepared breast injectant has the advantages that a colloid mill is adopted for grinding a part of materials, then a high-speed shearing and emulsifying machine is used for emulsifying and dispersing; oil suspension obtained by the combined use of the colloid mill and the high-speed shearing and emulsifying machine has the advantages of smaller particle size, higher uniformity and shorter grinding time when compared with oil suspension obtained by only adopting a stirring machine or a colloid mill in the traditional method; in addition, release amount of active ingredients and bioavailability are effectively increased, and the prepared breast injectant has a good treatment effect on a diary cow infected with diseases after delivery. The preparation method of the breast injectant is simple and the breast injectant is low in cost, so that the breast injectant is applicable to large-scale industrial production.
Description
Technical field
The present invention relates to a kind of milch cow lincomycin hydrochloride breast injection and preparation method thereof, belong to technical field of veterinary.
Background technology
Lincomycin (lincomycin) belongs to lincosamide antibiotics, has stronger antibacterial action to most of anaerobe and gram negative bacteria.Lincomycin acts on the ribosomal 50S subunit of sensitive organism, stops the prolongation of peptide chain, thus the protein synthesis of anti-bacteria cell.Lincomycin is generally antibacterial, but in higher concentrations, also has bactericidal action to high sensitive bacterial.
Existing lincomycin hydrochloride is mostly capsule, powder or water injection, stability, biocompatibility and effective ingredient burst size are all lower, cannot directly be injected by breast, not pointed to treatment milch cow puerperal infection, and existing commercially available milch cow lincomycin hydrochloride breast injection adopts after traditional each mixed raw material becomes colloidal sol, use colloid mill or high pressure homogenizer grinding, make oil suspension, its poor stability, poor biocompatibility, active constituent content is low, and therapeutic effect is undesirable.
Therefore, develop a kind of stability, biocompatibility and active constituent content high, and the milch cow lincomycin hydrochloride breast injection that therapeutic effect is good, treatment milch cow puerperal infection disease is significant.
Summary of the invention
First object of the present invention is, provides a kind of stability, biocompatibility and active constituent content high milch cow lincomycin hydrochloride breast injection.
The present invention's second object is, provides the preparation method of a kind of milch cow lincomycin hydrochloride breast injection.
To achieve these goals, the technical solution used in the present invention is as follows:
A kind of milch cow lincomycin hydrochloride breast injection, component containing following quality in every 100ml injectant: lincomycin hydrochloride 4.0 ~ 8.0g, suspending agent 1.0 ~ 3.0g, surfactant 0.2 ~ 3.0g, antioxidant 0.001 ~ 0.15g, antiseptic 1 ~ 5ml, surplus is injection soybean oil.
Described suspending agent is any one or the combination of aluminium stearate, sodium carboxymethyl cellulose, castor oil hydrogenated, polyvinylpyrrolidone, Polyethylene Glycol, microcrystalline Cellulose and sodium carboxymethyl cellulose complex RC-A591NF.
Described Polyethylene Glycol is polyethylene glycol 6000 or Macrogol 4000.
Described surfactant is any one or the combination of lecithin or Span-80.
Described antioxidant is oil phase antioxidant.
Described antioxidant is any one or combination in a-tocopherol, propyl gallate, butylated hydroxyarisol BHA, dibenzylatiooluene BHT, ascorbyl palmitate.
Described antiseptic is benzyl alcohol.
Preferably, a kind of milch cow lincomycin hydrochloride injectant, every 100mL contains the component of following quality: lincomycin hydrochloride 4 ~ 8g, aluminium stearate 1-3g, Span-80 0.1 ~ 1.5g, a-tocopherol 0.001 ~ 0.15g, soybean lecithin 0.1 ~ 1.5g, benzyl alcohol 1 ~ 5ml, surplus is injection soybean oil.
Preferred, a kind of milch cow lincomycin hydrochloride breast injection, every 100mL contains the component of following quality: lincomycin hydrochloride 6g, aluminium stearate 2g, Span-80 0.5g, a-tocopherol 0.1g, soybean lecithin 1.2g, benzyl alcohol 3ml, surplus is injection soybean oil.
The above-mentioned milch cow preparation method of lincomycin hydrochloride breast injection, concrete operation step is as follows:
1) get the sterile injection soybean oil of full dose 20%, heated and stirred, adds suspending agent simultaneously, insulation, forms factice A;
2) separately get the injection soybean oil of full dose 10%, heated and stirred, add lincomycin hydrochloride, antioxidant, surfactant simultaneously, insulation, form factice B;
3) factice B is ground;
4) factice A is poured in the factice B after grinding, pour into while stir, after stirring evenly, add antiseptic, and with injection soybean oil standardize solution, emulsifying, to obtain final product.
Step 3) described in be ground to colloid mill grinding.
Step 1) and step 2) described in heating-up temperature be 115 ~ 125 DEG C, temperature retention time is 20 ~ 40 minutes.
Step 4) described in emulsifying adopt high-speed shearing emulsion machine, the emulsified stirring of 6000 ~ 10000r/min rotating speed 0.5 ~ 1 hour.
Suspending agent sodium carboxymethyl cellulose has the effect of thickening agent simultaneously, and soybean oil synergism increases its emulsibility, improves the stability of suspension, biocompatibility and slow release effect.
Suspending agent polyvinylpyrrolidone is the macromolecular compound that polymerization generation occurs NVP, and reaction system viscosity is large, and polymer is not easy diffusion, polymerization reaction heat, improves the tolerance hot pressing of suspension.
Suspending agent PEG-4000 and PEG-6000 can increase the denseness of suspension, oil for injection and principal agent is not easily disperseed, prevents microparticles settle.
Suspending agent RC-A591NF, makes the mixing homogeneity of product improve, good biocompatibility, and slow release effect strengthens.
Milch cow of the present invention lincomycin hydrochloride breast injection, by lincomycin hydrochloride, surfactant, suspending agent, antioxidant, antiseptic and the composite composition of injection soybean oil, wherein lincomycin hydrochloride is the active component of breast injection, while the antibacterial action ensureing medicine, add surfactant and suspending agent, synergism, ingredient is uniformly dispersed, concordance is high, and medicine is present in oil phase with solid particle formation, sedimentation volume ratio is greater than 0.9, not easily there is caking phenomenon, stability and good biocompatibility, long action time, slow release effect is remarkable.
The preferred suspending agent aluminium stearate of injectant of the present invention, the composite use of surfactant Span-80, soybean lecithin, common synergism, delays and prevents microgranule from increasing, and the biocompatibility of suspension is greatly improved, and stablizes and strengthens mutually; The present invention's preferred oil phase antioxidant alpha-tocopherol, prevents lincomycin hydrochloride variable color in oil phase, increases the stability of product; The preferred benzyl alcohol of the present invention is as antiseptic, benzyl alcohol is as fat solvent simultaneously, the stability of product can be improved, and benzyl alcohol have preventing local relieve the pain pain effect, reduce the pain of milch cow, strengthen the therapeutic effect of medicine, preferred aluminium stearate, Span-80, soybean lecithin, alpha-tocopherol, the common synergism of benzyl alcohol in the present invention, while raising injectant stability and biocompatibility, do not affect the antibacterial action of medicine.
Preparation method of the present invention adopts prepares suspending agent factice and lincomycin hydrochloride, antioxidant, surfactant mixing factice respectively, and after the grinding of factice use colloid mill will be mixed, mix with suspending agent factice, adopt high-efficient shear emulsifying agent emulsion dispersion again, compare traditional preparation technology, oil suspension " particle diameter " prepared by the present invention is less, uniformity is higher, reduce milling time, effective ingredient burst size and bioavailability effectively improve, and have good therapeutic effect to milch cow puerperal infection disease.The preparation method of lincomycin hydrochloride breast injection of the present invention is simple, cost is low, is suitable for large-scale industrial production.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, but does not form any limitation of the invention.
Embodiment 1
Milch cow lincomycin hydrochloride breast injection in the present embodiment, component containing following quality in every 100mL injectant: lincomycin hydrochloride 6.0g, aluminium stearate 2.0g, Span-80 1.0g, a-tocopherol 0.05g, soybean lecithin 0.5g, benzyl alcohol 1.0ml, surplus is injection soybean oil.
The preparation method of the present embodiment milch cow lincomycin hydrochloride breast injection is that concrete operation step is as follows:
1) get the sterilized injection soybean oil of recipe quantity 20%, temperature is risen to 120 DEG C, heated and stirred, add sodium carboxymethyl cellulose simultaneously, clear gel system to be formed, stop stirring, keep stopping heating after 30 minutes, form factice A;
2) the sterilized injection soybean oil of recipe quantity 10% is got, temperature is risen to 125 DEG C, heated and stirred, add lincomycin hydrochloride, a-tocopherol, soybean lecithin, Span-80, treats that soybean lecithin melts and is uniformly dispersed into henna liquid, stops stirring, keep stopping heating after 30 minutes, form solution B;
3) factice B is ground 2 times in colloid mill;
4) factice A is poured in factice B, pour into while stir, after stirring, add benzyl alcohol again, after stirring evenly, add the injection soybean oil standardize solution of surplus, then high-speed shearing emulsion machine, the emulsified stirring of 6000r/min rotating speed 1 hour.
Embodiment 2
Milch cow lincomycin hydrochloride breast injection in the present embodiment, every 100mL contains the component of following quality: lincomycin hydrochloride 6.0g, PEG-40001.0g, Span-80 0.5g, a-tocopherol 0.1g, soybean lecithin 1.2g, benzyl alcohol 3.0ml, surplus is injection soybean oil.
The preparation method of the present embodiment milch cow lincomycin hydrochloride breast injection is that concrete operation step is as follows:
1) get the sterilized injection soybean oil of recipe quantity 20%, temperature is risen to 115 DEG C, heated and stirred, add PEG-4000 simultaneously, clear gel system to be formed, be i.e. factice, stop stirring, keep stopping heating after 40 minutes, form factice A;
2) the sterilized injection soybean oil of recipe quantity 10% is got, temperature is risen to 120 DEG C, heated and stirred, add lincomycin hydrochloride, a-tocopherol, soybean lecithin, Span-80, treat that soybean lecithin melts and is uniformly dispersed into henna liquid, stop stirring, keep stopping heating after 25 minutes, form solution B;
3) factice B is ground 1 time in colloid mill;
4) factice A is poured in factice B, pour into while stir, after stirring, add benzyl alcohol again, after stirring evenly, add the injection soybean oil standardize solution of surplus, then high-speed shearing emulsion machine, the emulsified stirring of 8000r/min rotating speed 0.8 hour.
Embodiment 3
The present embodiment milch cow lincomycin hydrochloride breast injection, every 100mL contains the component of following quality: lincomycin hydrochloride 6.0g, aluminium stearate 2.0g, PEG-4000 1.0g, RC-A591NF 3.0g, lecithin 0.2g, ascorbyl palmitate 0.001g, benzyl alcohol 5ml, surplus is injection soybean oil.
The preparation method of the present embodiment milch cow lincomycin hydrochloride breast injection is that concrete operation step is as follows:
1) get the sterilized injection soybean oil of recipe quantity 20%, temperature is risen to 125 DEG C, heated and stirred, add PEG-4000 simultaneously, clear gel system to be formed, be i.e. factice, stop stirring, keep stopping heating after 20 minutes, form factice A;
2) the sterilized injection soybean oil of recipe quantity 10% is got, temperature is risen to 115 DEG C, heated and stirred, add lincomycin hydrochloride, a-tocopherol, soybean lecithin, Span-80, treat that soybean lecithin melts and is uniformly dispersed into henna liquid, stop stirring, keep stopping heating after 40 minutes, form solution B;
3) factice B is ground 1 time in colloid mill;
4) factice A is poured in factice B, pour into while stir, after stirring, add benzyl alcohol again, after stirring evenly, add the injection soybean oil standardize solution of surplus, then high-speed shearing emulsion machine, the emulsified stirring of 10000r/min rotating speed 0.5 hour.
Embodiment 4
The present embodiment milch cow lincomycin hydrochloride breast injection, every 100mL contains the component of following quality: lincomycin hydrochloride 4.0g, sodium carboxymethyl cellulose 1.0g, soybean lecithin 1.5g, Span-80 0.5g, butylated hydroxyarisol 0.02g, benzyl alcohol 4ml, surplus is injection soybean oil.
The preparation method of the present embodiment milch cow lincomycin hydrochloride breast injection is that concrete operation step is as follows:
1) get the sterilized injection soybean oil of recipe quantity 20%, temperature is risen to 115 DEG C, heated and stirred, add PEG-4000 simultaneously, clear gel system to be formed, be i.e. factice, stop stirring, keep stopping heating after 30 minutes, form factice A;
2) the sterilized injection soybean oil of recipe quantity 10% is got, temperature is risen to 120 DEG C, heated and stirred, add lincomycin hydrochloride, a-tocopherol, soybean lecithin, Span-80, treat that soybean lecithin melts and is uniformly dispersed into henna liquid, stop stirring, keep stopping heating after 28 minutes, form solution B;
3) factice B is ground 1 time in colloid mill;
4) factice A is poured in factice B, pour into while stir, after stirring, add benzyl alcohol again, after stirring evenly, add the injection soybean oil standardize solution of surplus, then high-speed shearing emulsion machine, the emulsified stirring of 7000r/min rotating speed 0.6 hour.
Embodiment 5
The present embodiment milch cow lincomycin hydrochloride breast injection, every 100mL contains the component of following quality: lincomycin hydrochloride 8.0g, sodium carboxymethyl cellulose 3.0g, lecithin 3.0, dibenzylatiooluene 0.15g, benzyl alcohol 2ml, and surplus is injection soybean oil.
The preparation method of the present embodiment milch cow lincomycin hydrochloride breast injection is that concrete operation step is as follows:
1) get the sterilized injection soybean oil of recipe quantity 20%, temperature is risen to 115 DEG C, heated and stirred, add PEG-4000 simultaneously, clear gel system to be formed, be i.e. factice, stop stirring, keep stopping heating after 30 minutes, form factice A;
2) the sterilized injection soybean oil of recipe quantity 10% is got, temperature is risen to 120 DEG C, heated and stirred, add lincomycin hydrochloride, a-tocopherol, soybean lecithin, Span-80, treat that soybean lecithin melts and is uniformly dispersed into henna liquid, stop stirring, keep stopping heating after 28 minutes, form solution B;
3) factice B is ground 1 time in colloid mill;
4) factice A is poured in factice B, pour into while stir, after stirring, add benzyl alcohol again, after stirring evenly, add the injection soybean oil standardize solution of surplus, then high-speed shearing emulsion machine, the emulsified stirring of 7000r/min rotating speed 0.6 hour.
Embodiment 6
The present embodiment milch cow lincomycin hydrochloride breast injection, every 100mL contains the component of following quality: lincomycin hydrochloride 6.0g, aluminium stearate 2.0g, Span-80 0.5g, alpha-tocopherol 0.1g, soybean lecithin 1.2g, benzyl alcohol 3ml, surplus is injection soybean oil.
The preparation method of the present embodiment milch cow lincomycin hydrochloride breast injection is that concrete operation step is as follows:
1) get the sterilized injection soybean oil of recipe quantity 20%, temperature is risen to 120 DEG C, heated and stirred, add PEG-4000 simultaneously, clear gel system to be formed, be i.e. factice, stop stirring, keep stopping heating after 35 minutes, form factice A;
2) the sterilized injection soybean oil of recipe quantity 10% is got, temperature is risen to 120 DEG C, heated and stirred, add lincomycin hydrochloride, a-tocopherol, soybean lecithin, Span-80, treat that soybean lecithin melts and is uniformly dispersed into henna liquid, stop stirring, keep stopping heating after 30 minutes, form solution B;
3) factice B is ground 1 time in colloid mill;
4) factice A is poured in factice B, pour into while stir, after stirring, add benzyl alcohol again, after stirring evenly, add the injection soybean oil standardize solution of surplus, then high-speed shearing emulsion machine, the emulsified stirring of 9000r/min rotating speed 0.6 hour.
Test example
1, content detection and the long-time stability investigation of lincomycin hydrochloride breast injection is prepared in this test to embodiment 1 ~ 6
Test method: lincomycin hydrochloride breast injection prepared by Example 1 ~ 6, carry out content detection and long-time stability investigation respectively with reference to " veterinary drug national standard compilation-veterinary drug provincial standard rising national standard " (the 3rd), the results are shown in Table 1.
Table 1 breast injection content detection and long-time stability investigate result
| Sample | Content detection | Character | Room temperature deposits 6 months |
| Embodiment 1 | 100.1％ | Faint yellow oleagenous suspension | Content 99.0%, character are constant, settling ratio 0.96 |
| Embodiment 2 | 99.8％ | Faint yellow oleagenous suspension | Content 98.5%, character are constant, settling ratio 0.95 |
| Embodiment 3 | 98.2％ | Faint yellow oleagenous suspension | Content 99.0%, character are constant, settling ratio 0.97 |
| Embodiment 4 | 99.3％ | Faint yellow oleagenous suspension | Content 99.1%, character are constant, settling ratio 0.96 |
| Embodiment 5 | 100.5％ | Faint yellow oleagenous suspension | Content 99.7%, character are constant, settling ratio 0.97 |
| Embodiment 6 | 100.1％ | Faint yellow oleagenous suspension | Content 99.8%, character are constant, settling ratio 0.99 |
2, clinical trial
The breast injection treatment milch cow of preparing by the embodiment of the present invention 1 ~ 6 is by responsive microbial clinic mastitis, recessive mastitis and chronic diseases such as golden yellow coccus, streptococcus agalactiae, streptococcus dysgalactiaes.
Mammitis of cow clinical criteria:
1, clinic mastitis: be the inflammation of breast interstitial, essence or interstitial parenchymal tissue.It is characterized in that milk degeneration, breast tissue presents swelling, warm and pain to some extent.Different with coincident with severity degree of condition according to course of disease length, can the most acute, acute, subacute and chronic mammitis be divided into.The most acute: suddenly, development rapidly, is multiplely born in 1 Ge Ru district in morbidity.
2, latent mammitis: also known as Subclinical mastitis.For a kind of mastitis showed without clinical symptoms.It is characterized in that breast and milk without naked eyes as seen extremely, but milk changes on physicochemical property, bacteriology.Concrete manifestation pH value more than 7.0, in meta-alkalescence, has lump in breast, floccule, fiber in breast, sodium chloride content is more than 0.14%, and somatic number is at 500,000/more than ml, and bacterial population and electric conductivity value increase.
3, chronic disease: because mammary gland tissue is progressive inflammation process, lactogenic acinus wrecks in a big way, and mammary gland tissue generation fibrosis, often causes mastatrophy and induration of breast.
Laboratory animal: Jiaozuo City cattle farm, 210 Henan Province is cow breast intimitis through clinical diagnosis.
Experiment group technology: the trouble Niu Suiji being diagnosed as mastitis is divided into 6 groups of experimental grouies and 1 group of matched group, every 30 first groups.
Experimental administration mode: experimental group: 6 groups of experimental group milch cows adopt the breast injection of the embodiment of the present invention 1 ~ 6 respectively, sealing is connected to deferent duct by drug infusion in breast after cutting, and after milking, each one, newborn district, one day twice, is used in conjunction 2-3 time;
Matched group: each one, newborn district after adopting commercially available lincomycin hydrochloride breast injection to milk, one day twice, is used in conjunction 2-3 time.
Curative effect diagnostic criteria: observe at any time after medication and suffer from cattle performance, milk yield change, mammary secretion discharge situation, and carry out examination per rectum.Breed after oestrusing in good time, join and carry out pregnancy diagnosis in latter 3 months, and by following standard determination curative effect:
(1) clinical cure: udder size, shape and quality recover normal, and contractile response is normal.
(2) clinical improvement: udder size, shape recover normal substantially, and contractile response is still poor, the mucus of discharging when oestrusing is mixed with a small amount of flocculus, and breeding can not be conceived.
(3) clinical invalid: udder size, shape are substantially unchanged, contractile response is faint or ungauged regions reaction still, still discharges Catarrhal or mucositis mycopus when oestrusing.
Result of the test is as shown in table 2:
The zooperal clinical treatment outcome of this test example of table 2
Above-mentioned result of the test shows: experimental group cure rate is 77 ~ 90%, higher than matched group by 10 ~ 23%; Experimental group treatment total effective rate is 93 ~ 97%, and matched group total effective rate is 93%; The medication of experimental group milch cow can find to be clearly better for 2 ~ 3 days, and cow feeding amount, milk yield significantly rise, and the milch cow of more than 77% can be made to cure, and cures rear 3 non-return rates up to 83 ~ 90% (see table 1).As can be seen here, use breast injection of the present invention to treat mammitis of cow, have effective, instant effect, the advantages such as cure rate is high, can significantly improve the economic benefit of cattle farm.
Claims (10)
1. a milch cow lincomycin hydrochloride breast injection, it is characterized in that, component containing following quality in every 100ml injectant: lincomycin hydrochloride 4.0 ~ 8.0g, suspending agent 1.0 ~ 3.0g, surfactant 0.2 ~ 3.0g, antioxidant 0.001 ~ 0.15g, antiseptic 1.0 ~ 5.0ml, surplus is injection soybean oil.
2. described milch cow lincomycin hydrochloride breast injection as claimed in claim 1, it is characterized in that, described suspending agent is any one or combination of aluminium stearate, sodium carboxymethyl cellulose, castor oil hydrogenated, polyvinylpyrrolidone, Polyethylene Glycol, microcrystalline Cellulose and sodium carboxymethyl cellulose complex.
3. milch cow lincomycin hydrochloride breast injection as claimed in claim 1 described, is characterized in that, described surfactant is any one or the combination of lecithin or Span-80.
4. described milch cow lincomycin hydrochloride breast injection as claimed in claim 1, it is characterized in that, described antioxidant is any one or combination in a-tocopherol, propyl gallate, butylated hydroxyarisol, dibenzylatiooluene, ascorbyl palmitate.
5. described milch cow lincomycin hydrochloride breast injection as claimed in claim 1, it is characterized in that, described antiseptic is benzyl alcohol.
6. a milch cow lincomycin hydrochloride breast injection, it is characterized in that, every 100mL contains the component of following quality: lincomycin hydrochloride 4.0 ~ 8.0g, aluminium stearate 1.0 ~ 3.0g, Span-800.1 ~ 1.5g, a-tocopherol 0.001 ~ 0.15g, soybean lecithin 0.1 ~ 1.5g, benzyl alcohol 1.0 ~ 5.0ml, surplus is injection soybean oil.
7. described milch cow lincomycin hydrochloride breast injection as claimed in claim 6, it is characterized in that, every 100mL contains the component of following quality: lincomycin hydrochloride 6.0g, aluminium stearate 2.0g, Span-800.5g, a-tocopherol 0.1g, soybean lecithin 1.2g, benzyl alcohol 3.0ml, surplus is injection soybean oil.
8. the preparation method for lincomycin hydrochloride breast injection of the milch cow as described in claim 1 or 6, it is characterized in that, concrete operation step is as follows:
1) get the sterile injection soybean oil of full dose 20%, heated and stirred, adds suspending agent simultaneously, insulation, forms factice A;
2) separately get the injection soybean oil of full dose 10%, heated and stirred, add lincomycin hydrochloride, antioxidant, surfactant simultaneously, insulation, form factice B;
3) factice B is ground;
4) factice A is poured in the factice B after grinding, pour into while stir, after stirring evenly, add antiseptic, and with injection soybean oil standardize solution, emulsifying, to obtain final product.
9. the milch cow preparation method of lincomycin hydrochloride breast injection as claimed in claim 8, is characterized in that, step 1) and step 2) described in heating-up temperature be 115 ~ 125 DEG C, temperature retention time is 20 ~ 40 minutes.
10. the milch cow preparation method of lincomycin hydrochloride breast injection as claimed in claim 8, is characterized in that, step 4) described in emulsifying adopt high-speed shearing emulsion machine, the emulsified stirring of 6000 ~ 10000r/min rotating speed 0.5 ~ 1 hour.
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| CN104622803A (en) * | 2015-03-09 | 2015-05-20 | 邳州正康生物技术有限公司 | Breast injectant for treating mastitis of lactating dairy cows and preparation method of breast injectant |
| CN105597082A (en) * | 2016-01-29 | 2016-05-25 | 河南金大众生物工程有限公司 | Dairy cow breast injectant containing antimicrobial peptide and preparing method thereof |
| CN115715760A (en) * | 2022-12-22 | 2023-02-28 | 河北科星药业有限公司 | Preparation method of breast injection |
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| CN104622803A (en) * | 2015-03-09 | 2015-05-20 | 邳州正康生物技术有限公司 | Breast injectant for treating mastitis of lactating dairy cows and preparation method of breast injectant |
| CN105597082A (en) * | 2016-01-29 | 2016-05-25 | 河南金大众生物工程有限公司 | Dairy cow breast injectant containing antimicrobial peptide and preparing method thereof |
| CN105597082B (en) * | 2016-01-29 | 2019-05-31 | 河南金大众生物工程有限公司 | A kind of cow breast injectant and preparation method thereof containing antibacterial peptide |
| CN115715760A (en) * | 2022-12-22 | 2023-02-28 | 河北科星药业有限公司 | Preparation method of breast injection |
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Application publication date: 20150304 |