CN104606218A - Breast injection for treating cow lactation mastitis and preparation method thereof - Google Patents

Abstract

The invention discloses a breast injection for treating cow lactation mastitis and a preparation method thereof, and belongs to the technical field of preparation of veterinary drugs. The breast injection comprises lincomycin hydrochloride serving as an effective component. The breast injection is prepared from the following raw materials in percentage by weight: 5% of lincomycin hydrochloride, 6.25-22.5% of an emulgator, 5-26.25% of a wetting agent, 1-2% of a suspending aid and the balance of an oil-soluble medium. The preparation method of the breast injection comprises the following steps: uniformly mixing Tween-80 with glycerol firstly, then adding the mixture and aluminium stearate into a colloid mill to be uniformly mixed, finally adding the raw materials and the oil-soluble medium to be circularly milled so as to prepare a suspension. According to the breast injection, the dosage of the breast injection is easy to control; the action time of the injection can be retained; the breast injection is convenient in clinical application, remarkable in effect, high in cure rate and free of toxic and side effects, and can be used for effectively treating cow lactation mastitis caused by gram-positive bacteria and part of negative bacteria.

Description

A kind ofly treat breast injection of Lactation of Dairy Cow phase mastitis and preparation method thereof

Technical field

The present invention relates to a kind of breast injection, specifically a kind ofly treat breast injection of Lactation of Dairy Cow phase mastitis and preparation method thereof, be applicable to the mammitis of cow that gram-positive bacterium causes, belong to veterinary drug preparing technical field.

Background technology

Mastitis is a kind of multiple diseases seen of milch cow, and nearly all cattle farm, the whole world all exists this disease, causes huge loss to cow estate.

Mastitis is that caused by the microorganism of mammary gland tissue pathogenic infection or mammary gland tissue inflammatory damage, pathogenic microorganism mainly contains antibacterial, fungus, virus, mycoplasma etc., but comparatively general with bacteriological infection, and common bacteria has streptococcus, staphylococcus and intestinal bacteria; Inflammatory damage comprises long period lactogenic, various physicochemical stimulating factors etc.For the treatment of mammitis of cow, mainly adopt antibiotherapy both at home and abroad at present, have developed multiple special preparation, and define sensitive period and off-drug period, the control of this disease is played a good role; But mostly what occur in the market is compound antibiotic preparation, there is each component dosage to be difficult to control and to be unfavorable for the shortcomings such as the consequences such as veterinary's prescription, complicated components easily cause that calver is miscarried, external compound product be expensive, therefore, the control being still necessary for domestic mammitis of cow provides the better more special antibiotic injectant of folk prescription mastitis.

Summary of the invention

For the technical problem that above-mentioned prior art exists, the invention provides a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis, be easy to Charge control, the action time of medicine can be maintained, Clinical practice is convenient, and drug effect is remarkable, and cure rate is high, have no side effect, effectively can treat the Lactation of Dairy Cow phase mastitis that gram positive bacteria and part negative bacterium cause.

To achieve these goals, the effective ingredient of the breast injection of this treatment Lactation of Dairy Cow phase mastitis is lincomycin hydrochloride;

Calculate formulated by following raw materials by weight:

Hydrochloric acid woods Mycosporin: 5%;

Emulsifying agent: 6.25%-22.5%;

Wetting agent: 5%-26.25%;

Suspending agent: 1%-2%;

Oil solvent: surplus.

Further, described emulsifier tween is 85,80,65,60.

As preferably of the present invention, the optimum tween of described emulsifying agent is 80.

Further, described wetting agent is one or both in glycerol, oxireme-40-castor oil hydrogenated.

Further, described suspending agent is one or both in aluminium stearate, vaseline, Carboxymethyl cellulose sodium, xanthan gum, castor oil hydrogenated.

Further, described oily solvent is one or both in injection soybean oil, ethyl oleate, liquid paraffin, glyceryl triacetate, injection Oleum Ricini.

As preferably of the present invention, described oily solvent is injection soybean oil.

Invention also provides a kind of preparation method for the treatment of the breast injection of Lactation of Dairy Cow phase mastitis, its concrete steps are as follows:

(1), by both emulsifying agent and wetting agent mix, stir, heat to the two and merge completely;

(2), by the homogeneous mixture of emulsifying agent and wetting agent add colloid mill, then add the suspending agent of formula ratio, start colloid mill and grind;

(3), after step (2) ground and mixed is even, adds the lincomycin hydrochloride of formula ratio, and add oily solvent to formula ratio, carry out circular grinding;

(4), be ground to particle diameter when being all less than 50 μm, stop grinding, both the breast injection of described treatment Lactation of Dairy Cow phase mastitis;

(5), fill, sterile sealing preserve.

Compared with prior art,

1, this breast injection is single preparations of ephedrine, and all have good effect to pathogenic bacterium such as causing the gram positive bacteria of mastitis and multiple negative bacterium, being convenient to veterinary carries out prescription therapeutic mammitis of cow, is easy to control dosage;

2, in this breast injection, major pharmaceutical component is lincomycin hydrochloride, there is good dissolubility, be conducive to absorbing in cow mammary gland tissue, and utilize oily mediator to make suspension to produce certain slow releasing function, good stability, can maintain the action time of medicine;

3, this breast injection is oiliness mixed suspension preparation, and product has good flexibility, good evenness, and granularity is little, action effect is fast, and Clinical practice is convenient.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention will be further described.

The effective ingredient of the breast injection of this treatment Lactation of Dairy Cow phase mastitis is lincomycin hydrochloride;

Calculate formulated by following raw materials by weight:

Hydrochloric acid woods Mycosporin: 5%;

Emulsifying agent: 6.25%-22.5%;

Wetting agent: 5%-26.25%;

Suspending agent: 1%-2%;

Oil solvent: surplus.

Further, preferred as a modification of the present invention, the optimum tween of described emulsifying agent is 80.

In substrate, tween is divided into polysorbate85,80,65,60 according to esters difference, and wherein Tween 80 can play the effect of emulsifying, dispersion, solubilising admirably in this product, and effect is better than other several model.

Further, described wetting agent is one or both in glycerol, oxireme-40-castor oil hydrogenated.

Further, described suspending agent is one or both in aluminium stearate, vaseline, Carboxymethyl cellulose sodium, xanthan gum, castor oil hydrogenated.

Further, described oily solvent is one or both in injection soybean oil, ethyl oleate, liquid paraffin, glyceryl triacetate, injection Oleum Ricini.

As preferably of the present invention, described oily solvent is injection soybean oil.

Invention also provides a kind of preparation method for the treatment of the breast injection of Lactation of Dairy Cow phase mastitis, its concrete steps are as follows:

(1), by both emulsifying agent and wetting agent mix, stir, heat to the two and merge completely;

(2), by the homogeneous mixture of emulsifying agent and wetting agent add colloid mill, then add the suspending agent of formula ratio, start colloid mill and grind;

(3), after step (2) ground and mixed is even, adds the lincomycin hydrochloride of formula ratio, and add oily solvent to formula ratio, carry out circular grinding;

(4), be ground to particle diameter when being all less than 50 μm, stop grinding, both the breast injection of described treatment Lactation of Dairy Cow phase mastitis;

(5), fill, sterile sealing preserve.

Embodiment one:

Embodiment two:

Embodiment three:

Embodiment four:

The breast injection made by formulation ratio according to the various embodiments described above carries out curative effect of medication experiment:

Somatic number in milk can reflect the health status of breast tissue, so this experiment is by measuring somatic number evaluate efficacy in milk.Concrete principle is as follows: when there is mastitis in breast, and milk somatic cell number can increase, and the more serious somatic number of inflammatory conditions is higher.

1, experiment material:

Blank group: normal saline;

Drug control group: the breast injection for the treatment of Lactation of Dairy Cow phase mastitis of the present invention;

Randomly draw certain cattle farm milk cow in lactation period 200.

2, method:

200 cow heads randomly drawed are divided into matched group and experimental group, often organize each 100, after squeezing clean intramammary residual milk the last time, the cow breast of experimental group is poured into the breast injection for the treatment of Lactation of Dairy Cow phase mastitis of the present invention, every is injected 5ml; The normal saline of matched group milch cow perfusion equal quantities, 10d, 15d, 20d first time then after calving milks sampling 5ml, adopts the Li La of DeLaval Company to cut down the change that somatic cell detector measures wherein somatic number.

3, result:

Table 1

Result shows: as shown in table 1, and on somatic number, experimental group is lower than matched group by 31.37%, and on clinical incidence of mammits, experimental group is lower than matched group by 64.7%.Illustrate that the breast injection for the treatment of Lactation of Dairy Cow phase mastitis of the present invention can reduce the incidence rate of mammitis of milk cow in lactation period significantly, and there is good curative effect.

The breast injection made by formulation ratio according to the various embodiments described above carries out In vitro Bactericidal Experiments:

1, experimental strain:

Streptococcus, staphylococcus aureus, streptococcus pyogenes, Salmonella.

2, bacterium solution preparation:

The antibacterial of kind of the lyophilizing of four described in 1 is accessed in four agar culture mediums, 24h is cultivated in 37 DEG C, activation, picking colonies typical, be inoculated in respectively in broth medium, firmly jolting uses bacterium to be uniformly distributed in meat soup, is placed in 37 DEG C of calorstats and cultivates 24h, use physiological saline solution diluted for use after taking out.

3, the dilution of medicine:

Physiological saline solution is adopted this product to be diluted to antibacterial medicines concentration for subsequent use.

4, bacteriostatic test:

Getting the test organism liquid that 0.1ml diluted adds in the culture dish of asepsis growth, make it evenly to be laid on agar surface, then sterilizing Oxford cup is placed in plate, in infusion of medicine Oxford cup 0.1ml diluted with sterilizing sample injector, plate is built, is placed in after 24h cultivated by 37 DEG C of calorstats and observes.

5, bacteriostatic experiment result:

Bacterial strain	Streptococcus	Staphylococcus aureus	Escherichia coli	Salmonella
					Diameter (mm)	24±0.35	25±0.56	20±0.33	19±0.36
Sensitivity	+++	+++	+++	+++

Table 2

Note: antibacterial circle diameter is less than 8mm, judges that this bacterium represents with "-" medicament is insensitive; Antibacterial circle diameter, between 8 ~ 10mm, judges that this bacterium is to quick in medicament, represents with " ++ ": antibacterial circle diameter, between 15 ~ 20mm, judges that this bacterium is to medicament Gao Min, represents with " +++ ".

From above-mentioned experimental result: the breast injection for the treatment of Lactation of Dairy Cow phase mastitis of the present invention is in vitro in bacteriostatic test, good fungistatic effect is all shown to streptococcus, staphylococcus aureus, escherichia coli, Salmonella, well can play the effect of bacteriostasis and sterilization.

Above-mentioned the disclosed embodiments, can enable those skilled in the art realize or use the present invention.Embodiment in the present invention, only for the present invention will be described, does not form the restriction to right, other actual equivalent substituting, all within protection scope of the present invention that those skilled in that art can design.

Claims (8)

1. treat a breast injection for Lactation of Dairy Cow phase mastitis, it is characterized in that,

The effective ingredient of described breast injection is lincomycin hydrochloride;

Calculate formulated by following raw materials by weight:

Hydrochloric acid woods Mycosporin: 5%;

Emulsifying agent: 6.25%-22.5%;

Wetting agent: 5%-26.25%;

Suspending agent: 1%-2%;

Oil solvent: surplus.

2. a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis according to claim 1, is characterized in that,

Described emulsifier tween is 85,80,65,60.

3. a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis according to claim 2, is characterized in that,

The optimum tween of described emulsifying agent is 80.

4. a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis according to claim 1, is characterized in that,

Described wetting agent is one or both in glycerol, oxireme-40-castor oil hydrogenated.

5. a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis according to claim 1, is characterized in that,

Described suspending agent is one or both in aluminium stearate, vaseline, Carboxymethyl cellulose sodium, xanthan gum, castor oil hydrogenated.

6. a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis according to claim 1, is characterized in that,

Described oily solvent is one or both in injection soybean oil, ethyl oleate, liquid paraffin, glyceryl triacetate, injection Oleum Ricini.

7. a kind of breast injection for the treatment of Lactation of Dairy Cow phase mastitis according to claim 1, is characterized in that,

Described oily solvent is injection soybean oil.

8. a kind of preparation method for the treatment of the breast injection of Lactation of Dairy Cow phase mastitis according to claim 1 to 7 any one, it is characterized in that, concrete steps are as follows:

(1), by both emulsifying agent and wetting agent mix, stir, heat to the two and merge completely;

(2), by the homogeneous mixture of emulsifying agent and wetting agent add colloid mill, then add the suspending agent of formula ratio, start colloid mill and grind;

(3), after step (2) ground and mixed is even, adds the lincomycin hydrochloride of formula ratio, and add oily solvent to formula ratio, carry out circular grinding;

(4), be ground to particle diameter when being all less than 50 μm, stop grinding, both the breast injection of described treatment Lactation of Dairy Cow phase mastitis;

(5), fill, sterile sealing preserve.