CN101507764B - Chinese herbal medicine spraying agent for treating milking-stock mastitis and preparation method thereof - Google Patents
Chinese herbal medicine spraying agent for treating milking-stock mastitis and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to an animal medicament, in particular to a Chinese herbal medicine spraying agent for treating garget of dairy stock and a preparation method thereof. The invention solves the problems of various means for treating the garget of the dairy stock. The Chinese herbal medicine spraying agent comprises the following raw medicines of effective compositions: frankincense, common myrrh, safflower and peach kernels. The preparation method comprises the following steps: preparing a concentrated solution 1, namely taking the raw materials in the formulation, namely the frankincense, the common myrrh, the safflower and the peach kernels, using ethanol for extraction, and concentrating the filtrate until each milliliter of the solution contains 2 grams of raw medicines; preparing a concentrated solution 2, namely taking Artemisia desertorum raw materials in the formulation, using the ethanol for extraction, and concentrating the filtrate until each milliliter of the solution contains 2 grams of raw medicines; and mixing the concentrated solution 1, the concentrated solution 2 and propylene glycol according to the mixture ratio of 4:1:1.2, adding the ethanol into the mixture until the gross amount is 1 gram of raw medicines per milliliter, uniformly mixing the mixture, and subpackaging the obtained solution into spraying containers. The Chinese herbal medicine spraying agent has the advantages of resisting bacteria and expelling superficial evils, reducing bloatedness and resolving masses, activating blood circulation to dissipate blood stasis, removing necrosis and promoting granulation, and freeing channels and benefiting breast, and is used for treating the garget of various animals such as milk cows, milk goats, pigs, horses, and the like.
Description
Technical field
The invention belongs to animal pharmaceuticals, particularly a kind of Chinese herbal medicine spray that is used for the treatment of milking-stock mastitis and preparation method thereof.
Background technology
Mastitis is one of major disease that seriously restricts the milk cattle cultivating industry.According to U.S. milk industry association statistics, clinical type mammitis of cow sickness rate is up to more than 50%, the paathogenic factor that causes mammitis of cow is more, and the infective pathogen complexity all can cause as external bad conditions such as antibacterial, fungus, mycete, chemicals, warm stimulation and mechanical damages.According to the interrelated data statistics, the mastitis of China milch cow all is to be caused by pathogenic microorganism more than 80%.The cause of disease speck that causes mammitis of cow is mainly the conditionality pathogenic bacterium, and especially based on staphylococcus, streptococcus, escherichia coli are also more common, and these three kinds of bacterial mastitiss account for more than 90% of total incidence.The medicine that the treatment mammitis of cow mainly uses has antibiotics, recombinant vaccine, reorganization cattle gamma interferon etc.
Antibiotics is a kind of treatment means that generally adopts both at home and abroad at present, mainly uses penicillin medicine, aminoglycoside medicaments, macrolide antibiotics and lincomycin etc. clinically. adopt methods such as intramuscular injection, intravenous injection or breast perfusion.These methods can both effectively be controlled the infection of pathogenic bacterium, but according to the literature, at the isolating pathogenic bacterium great majority of the present mastitis of China antibiotic commonly used are all produced drug resistance.For example: the applicant has separated 2 strain staphylococcuses from 22 routine clinic mastitis of cattle farm, Shaanxi Province, wherein staphylococcus aureus is to penicillin, ampicillin, benzylpencilline, fortum and the equal drug resistance of compound sulfanilamide, and staphylococcus epidermidis is to the equal drug resistance of penicillin, ampicillin, benzylpencilline, fortum, sulfanilamide, tetracycline and chloromycetin.Through investigation, with this mammitis of cow for many years sickness rate often use penicillins relevant than higher with the treatment of compound sulfanilamide.The mammitis of cow Drug-resistance of Staphylococcus all has report throughout the country, and quite serious.For example geographic mammitis of cow staphylococcus, escherichia coli, the streptococcus such as Heilungkiang of report such as the area, Sichuan of report such as the area, Huhehaote of report such as the Beijing area of report such as Zhang Zhongwen, Wang Bing, Dai Min and Wang Geng have all produced drug resistance to antimicrobial drugs such as penicillin, streptomycin and sulfonamides, and present multi-drug resistant.Because antibiotic abuse causes antibiotic remains and milch cow drug disposition savings in the milk, jeopardizes health.Along with people to food safety requirements improve constantly and country to the stricter requirement of quality of dairy products, the antibiotic that the clinical treatment mastitis is commonly used is owing to exist drug residue to be limited the use of or to forbid gradually.
Recombinant vaccine adopts the generation of vaccine control mastitis, it is safer, effective method, but because the variation of bacterial antigens, cause vaccine fine 1 year effect, 1 year is ineffective. and the pathogenic species class that causes mastitis in addition is many, pathogenesis is complicated, and present vaccine is difficult to various mastitiss are all played effect.
The reorganization cattle gamma interferon is a cytokine, mainly strengthens leukocyte and macrophage phagocytic ability by the promotion immunity of organisms, but does not influence its sterilizing ability, and mechanism of its treatment mammitis of cow it be unclear that.
Summary of the invention
The present invention provides a kind of clinic mastitis to dairy stock (particularly milch cow) that Chinese herbal medicine spray of good therapeutical effect and preparation method thereof is arranged for the problems referred to above that the various treatment meanss that solve existing milking-stock mastitis exist.
The present invention takes following technical scheme to realize:
The Chinese herbal medicine spray that is used for the treatment of milking-stock mastitis, the raw materials of effective components medicine and the percentage by weight thereof that make this medicine are: Olibanum 20%～25%, Myrrha 20%～25%, Flos Carthami 20%～25%, Semen Persicae 20%～25%, Folium et Cacumen Artemisiae Halodendri 10%～20%; The percentage by weight sum of above-mentioned raw materials medicine is 100%.
Be used for the treatment of the Chinese herbal medicine spray preparing process of milking-stock mastitis, its step is as follows:
1), get Olibanum, Myrrha, Flos Carthami and Semen Persicae raw material in the prescription, use ethanol extraction, filtrate be concentrated into every milliliter contain 2 the gram crude drugs, concentrated solution 1;
2), get the Folium et Cacumen Artemisiae Halodendri raw material in the prescription, use ethanol extraction, filtrate be concentrated into every milliliter contain 2 the gram crude drugs, concentrated solution 2;
3), the concentrated solution of above-mentioned preparation is cooperated concentrated solution 1 according to following ratio: concentrated solution 2: propylene glycol=4: 1: 1.2, add ethanol to total amount 1g crude drug/mL, mix homogeneously is sub-packed in automiser spray promptly.
Acute mastitis in the traditional Chinese veterinary medicine also claims that milk is yellow, and milk is swollen, and western veterinary is called mastitis, is the commonly encountered diseases of lactogenic animal, and frequently-occurring disease is had a strong impact on lactogenic output and the quality of dairy stock, is directly endangering the sound development of livestock breeding industry, dairy industry.Chinese herbal medicine belongs to natural product, and toxicity is low, can not cause serious drug residue, develop immunity to drugs. and it is the important component part of China's cultural heritage.Wherein Flos Carthami, Semen Persicae, Olibanum, Myrrha are called as the panacea for the treatment of wound, can be rated as the gold partner in the surgery medical material, Flos Carthami, Semen Persicae, Olibanum, Myrrha are early recorded by numerous Ancient Times in China doctor such as Compendium of Material Medica, " surgeon's complete works " and " must declare book on Chinese herbal medicine " allusion quotation, pharmacopeia.The Compendium of Material Medica cloud: " Flos Carthami is invigorated blood circulation, moisturizes, and pain relieving, it is swollen to loose, and stimulates the menstrual flow.The Semen Persicae blood circulation promoting and blood stasis dispelling is partial to local stasis of blood, and both Chang Xiangxu are for using "; " Olibanum is invigorated blood circulation, and the Myrrha blood that looses all can the blood-activating and qi-promoting blood stasis dispelling, relaxing muscles and tendons and activating QI and blood in the collateral pain relieving, detumescence and promoting granulation, uses so two medicines are often held concurrently mutually "." Medical Treasures of the Golden Chamber ": reddish blue flower wine side's tool blood circulation promoting competent silt, the merit of promoting the circulation of QI pain relieving." book on Chinese herbal medicine analyze mentally record ": Semen Persicae, main attack blood stasis and be the liver medicine is held concurrently and is dredged the stasis of blood of the skin natural fibre line of meat." Records of Tradition Chinese and Western Medicine in Combination " records: " Olibanum, Myrrha, two medicines and usefulness are logical internal organs, the key medicine of dredging the meridian, and the energy removing toxic substances and promoting subsidence of swelling, the granulation promoting pain relieving, really good medicine is also." abroad Olibanum more, have " desert Margarita ", " God's tear " and good reputations such as " white gold " because of its preciousness.Flos Carthami mainly contains carthamin, neocarthamin, Flos Carthami quinone glycoside, Flos Carthami polysaccharide, Palmic acid, cinnamic acid, lauric acid etc.Semen Persicae mainly contains amygdalin, emulsin, fatty wet goods.Olibanum contains cadinene, lauro lene, cinene, firpene, phellandrene, olibanol and resin, natural gum etc.; Myrrha mainly contains myrrholic acid, cinnamic aldehyde, Fructus Foeniculi aldehyde, eugenol, cadinene, firpene, cinene, Myrrha terpene, limonene and resin, natural gum etc.Wherein Olibanum has antibiotic, the flatulence that restrains, dispels, promotes that wound scabs, promotes effects such as cytoactive, its effect of invigorating blood circulation is stronger, and Myrrha has the tissue deterioration of preventing effect, the situation that the wound gangrene is especially arranged, its algefacient function can help furuncle, skin ulcer and skin ulcer, can also improve the wound of suppurating and the skin of be full of cracks.Act as the master with the blood that looses, the two all has the alleviating pain and detumescence effect.Olibanum, Myrrha have blood vessel dilating, analgesia, antiphlogistic effect.So the combination of these two kinds of Chinese medicines is all arranged greatly in the external preparation prescription of promoting blood circulation to remove blood stasis.Egyptian and Greek are considered as a kind of scarce commodity with Myrrha, and it is used for offering a sacrifice to gods or ancestors gods, celebrates fiesta, cosmetics, perfume and medical herbs, and the Egyptian more mixes Myrrha to reach purpose anticorrosion and that purify with Olibanum.Folium et Cacumen Artemisiae Halodendri has wind-damp dispelling, eliminating pus and toxicity.Treating rheumatic arthritis, flu, pharyngalgia, furuncle carbuncle." Chinese desert area medicinal plants ": Folium et Cacumen Artemisiae Halodendri leaf: mash the external application furuncle carbuncle opening that can suppurate ahead of time, the tool effect of drawing out the pus.
The Chinese herbal medicine spray of the treatment treatment mammitis of cow of the present invention's preparation is the brownish red supernatant liquid, and gas is dense, fragrant, has antibacterial and detoxicating, dispersing swelling and dissipating binds, blood circulation promoting and blood stasis dispelling, putrefaction-removing granulation-promoting, the sharp breast of collateral dredging.Be used for the treatment of various mammitis of animal such as milch cow, milk goat, pig and horse.Trade name is mastitis one spray spirit.
The present invention is on the Chinese medicine formula basis of tradition control mastitis, select traditional Chinese herbal medicine for use according to two ones of " Chinese veterinary drug allusion quotation " versions in 2005 and " veterinary drug import quality standard ", on the basis of clinical practice for many years, develop a kind of pure medicinal herb spray of transdermal therapeutic milking-stock mastitis.Through pharmacodynamics test and safety testing, respond well.Healthy and orderly development has crucial meaning to dairy, and using value and market prospect are wide.
The specific embodiment
Drug effect, pharmacological testing below in conjunction with medicine of the present invention are described in further detail embodiments of the invention.
1, prescription analysis
The present invention is that the applicant is according to veterinary drug production for many years and clinical experience, determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs theory according to Chinese veterinarian's medicine, and according to the principles of formulating prescriptions of traditional Chinese veterinary medicine, screening has the herbal prescription preparation that control has blood circulation promoting and blood stasis dispelling, reducing swelling and alleviating pain, anti-inflammation, wherein Flos Carthami has promoting blood circulation to restore menstrual flow, eliminating stasis to stop pain effect, and Semen Persicae has the blood circulation promoting and blood stasis dispelling effect, and Olibanum is invigorated blood circulation, the Myrrha blood effect of loosing, Folium et Cacumen Artemisiae Halodendri has heat-clearing and toxic substances removing, cooling blood for hemostasis effect.All medicines share, and have antibacterial and detoxicating, dispersing swelling and dissipating binds, blood circulation promoting and blood stasis dispelling, putrefaction-removing granulation-promoting, the sharp breast of collateral dredging.Be used for the treatment of various mammitis of animal such as milch cow, milk goat, pig and horse.
Mastitis one spray spirit has easy to use, and is more convenient than present clinical prevention mammitis of cow powder commonly used, injection and unguentum use, has no side effect.Directly sparge on the breast, using value and market prospect are boundless.
2, Pharmacodynamic test of active extract
2.1, extracorporeal bacteria inhibitor test (agar diffusion method)
Be prepared into the circular paper of 6mm with filter paper, be soaked in the described medicinal liquid of the invention described above, volatilize solvent, standby, establish the contrast of propylene glycol substrate group simultaneously.
Escherichia coli, staphylococcus aureus, staphylococcus epidermidis, streptococcus agalactiae and streptococcus dysgalactiae separate from Shaanxi Province and the clinical mastitis milk in part cattle farm, Shanxi Province by Shanxi Ruicheng Yabaoshouyao Co., Ltd.'s pharmaceutical science institute and carry out and identify, adopt agar diffusion method, observe antibacterial ring size and write down result, triplicate.Compare with the penicillin bacteriostatic test simultaneously.The results are shown in Table 1.
The one spray spirit of table 1 mastitis is to the antibacterial result (mm) of clinical mastitis isolate
Annotate: the no bacteriostasis of "-" expression.
2.2, the clever antiinflammatory action research of mastitis one spray
2.2.1, the influence of the clever xylol induced mice capillary of skin permeability of mastitis one spray
40 of mices, male and female half and half are available from The Fourth Military Medical University's Experimental Animal Center.Be divided into 4 groups (n=10).The back depilation, tail vein injection 0.5% azovan blue normal saline solution 0.3mL/ only, first group is matched group, spraying propylene glycol substrate, second group shifts to an earlier date the administration group, be the back Intradermal inject dimethylbenzene 0.03mL cause scorching before the one spray spirit of 10min spraying mastitis, the 3rd group causes the mastitis one spray spirit of spraying immediately after the inflammation for the back Intradermal injects dimethylbenzene 0.03mL, the 4th group sprays clever for mouse back Intradermal injection dimethylbenzene 0.03mL causes scorching back 10min spraying mastitis one, mice is put to death in dislocation behind the 20min, the back cropping, the skin that indigo plant is dyed is cut chopping and is put into 6mL acetone normal saline and soak 48h, the centrifugal 10min of 2000r/min, (λ=590nm) measures optical density value and writes down the result with 721 spectrophotometers to get supernatant.The results are shown in Table 2.
The clever influence to the mouse skin capillary permeability of table 2 mastitis one spray (x ± S)
Annotate: * represents to compare with matched group significant difference (P＜0.05); * represents to compare with matched group difference extremely significantly (P＜0.01);
△Significant difference (P＜0.05) is compared in expression with the 3rd group,
△ △Difference extremely significantly (P＜0.01) is compared in expression with the 4th group.
Table 2 is the result show, the 4th group has significant inhibitory effect to the mouse skin capillary permeability, and the 3rd group and second group of group have utmost point significant inhibitory effect to the mouse skin capillary permeability, and between each dosage group being proportionate property of notable difference are arranged.
2.2.2, the influence of the clever xylol induced mice auricular concha inflammation of mastitis one spray
40 mices, male and female half and half, random packet, the auricular concha administration, dosage is the same.Left side auricular concha melted paraxylene 0.02mL/ only dislocates behind the 30min and puts to death, and cuts two ears with shears along the auricle baseline of mice, and the perforator at same position with 6mm sweeps away auricle, and two auricles are weighed and asked poor, the results are shown in Table 3.
The influence of the clever xylol induced mice auricular concha of table 3 mastitis one spray swelling (x ± S)
| Group | Dosage/mL | Number of animals/only | Two auricles weight difference/μ g |
| Second group the 3rd group the 4th group of matched group | 0.1 0.1 0.1 0.1 | 10 10 10 10 | 2.10±0.66 0.35±0.15 **△△ 0.80±0.18 **△△ 1.55±0.41 * |
Annotate: * represents to compare with matched group significant difference (P＜0.05);
*Difference extremely significantly (p＜0.01) is compared in expression with matched group;
△ △Difference extremely significantly (p＜0.01) is compared in expression with the 4th group of group.
Table 3 is the result show, the 4th group has remarkable inhibitory action to mice auricular concha inflammation; The 3rd group and the 4th group of group have utmost point significant inhibitory effect to mice auricular concha inflammation, and second group has been compared extremely significant difference with the 3rd group with the 4th group.
2.2.3, mastitis one spray spirit is to the granulomatous influence of mice agar
40 mices, male and female half and half, be divided into 4 groups at random, back barium sulfide depilation 1cm * 1cm, each depilation district, Mus back subcutaneous injection 2% sterilization agar 0.2mL, the injection back is rounded, behind the 1h, at injection place percutaneous drug delivery, the propylene glycol substrate of spraying first group of every day 4 times, the mastitis one spray spirit 6 times of spraying second group of every day, the mastitis one spray spirit 4 times of spraying the 3rd group of every day, clever 2 times of the mastitis～spray of spraying the 4th group of every day is 0.1mL at every turn, mice is put to death in dislocation in the 8th day, cut skin of back, peel off complete granuloma, accurately take by weighing weight and write down the result, granuloma weight deducts former agar weight and asks poor, the results are shown in Table 4.
Table 4 mastitis one spray spirit is to the granulomatous influence of mice agar (x ± S)
Annotate:
*Significant difference (P＜0.05) is compared in expression with matched group;
*Difference extremely significantly (p＜0.01) is compared in expression with matched group;
△Significant difference (P＜0.05) is compared in expression with the 3rd group;
△ △Difference extremely significantly (P＜0.01) is compared in expression with the 4th group.
Table 4 is the result show, the 4th group has significant inhibitory effect to mice agar granuloma; Second group and the 3rd group have utmost point significant inhibitory effect to mice agar granuloma, and second group has the obvious suppression effect than other dosage group.
The result proves that mastitis one spray spirit has extremely significant antiinflammatory action, and with being proportionate property of dosage.For clinical practice provides sufficient test basis.
2.3, mastitis one spray spirit causes the influence of fever in rabbits reaction to Oleum Terebinthinae
1.5kg～2.0kg New Zealand white rabbit, 20, male and female have concurrently, are provided by Ruicheng County xx kind warren.The 3d that conforms, and the 3d adaptability is measured the rabbit rectal temperature continuously, chooses 38.5 ℃～39.5 ℃ of basal body temperatures, variations in temperature＜0.3 ℃ rabbit.Test same day is 1 hour thermometric 2 times at interval, average as body temperature before the administration, be divided into mastitis one spray miraculous cure thing group and propylene-glycol-based matter matched group at random, each is organized the rabbit abdominal part and loses hair or feathers with barium sulfide solution, area is 3cm * 3cm, the one spray spirit of medicine group spraying mastitis, matched group spraying propylene glycol substrate, every administration in 12 hours 1 time, totally 3 days.Every rabbit is at preceding 24 hours backs of last administration subcutaneous injection Oleum Terebinthinae 0.4mL/kg, rejects temperature＜0.8 ℃ rabbit, every 1 hour thermometric 1 time, amounts to 5 times each time point body temperature after 24 hours and the administration after the record pyrogenicity after the last administration.The results are shown in Table 5.
Table 5 mastitis one spray spirit causes (x ± S) ℃ of the influence of fever in rabbits to Oleum Terebinthinae
| Group | Temperature before the medicine | 24h body temperature | 1h | 2h | 3h | 4h | 5h |
| Matched group | 39.20±0.51 | 41.46±0.02 | 41.26±0.18 | 41.21±0.21 | 41.20±0.25 | 41.06±0.2 | 40.88±0.14 |
| The medicine group | 39.210±0.23 | 41.07±0.11** | 40.88±0.07* | 40.82±0.52 | 40.85±0.11* | 40.54±0.11* | 40.39±0.13* |
Annotate: compare with matched group
*P＜0.05,
*P＜0.01
As can be seen from Table 5, mastitis one spray spirit has tangible refrigeration function to fever in rabbits due to the Oleum Terebinthinae.Each time point body temperature all has reduction.
2.4, the analgesic activity research of mastitis one spray spirit
White mice, 40, Kunming mouse, 18～22g, female, provide by The Fourth Military Medical University's Experimental Animal Center.Earlier RCY-2 type hot plate dolorimeter (Shanghai benefit connection science and education equipment company) being regulated thermostat makes temperature control at 55 ± 0.5 ℃, choose female mice number subsequently, each one is placed on the hot plate, survey pain threshold, criterion is that white mice is placed on the hot plate to the metapedes required time occurring licking, discard pain threshold less than 5 seconds, greater than 30 seconds or leaper, choose 20 white mice, be divided into propylene-glycol-based matter matched group and mastitis one spray miraculous cure thing group at random, two metapedes spraying medicine, every administration in 12 hours 1 time, totally 3 days.Measure the pain threshold of 15min, 30min after the administration, 60min respectively.The results are shown in Table 6.
The clever influence to mice hot plate pain reaction of table 6 mastitis one spray (x ± S)
| Group | The example number/only | Before the medicine | 15min behind the medicine | 30min behind the medicine | 60min behind the medicine |
| Matched group | 10 | 7.36±1.63 | 8.27±1.89 | 9.33±2.57 | 10.86±3.45 |
| The medicine group | 10 | 7.40±1.86 | 9.46±2.52 | 12.11±2.32* | 14.10±3.73* |
Annotate: compare with matched group
*P＜0.05
Can find out that from table 6 mastitis one spray spirit causes mice pain reaction significant difference (P＜0.05) behind 30min to hot plate.
2.5, the toxicity test of mastitis one spray spirit
2.5.1, to the acute toxicity test of mice
40 of Kunming mouses, 18～22g, male and female half and half, each back spraying 0.2mL, every 2h once observed 3 days continuously.The result: it is good that each organizes mice growing state during acute toxicity testing, there is no abnormal response, do not see that animal has obvious poisoning symptom, do not have dead the generation.
2.5.2, to the chronic toxicity test of mice
40 of Kunming mouses, 18～22g, male and female half and half, each skin of back spraying 0.1mL once observed 3 months in per 12 hours continuously.The result: it is good that each organizes mice growing state during long term toxicity test, there is no abnormal response, do not see that animal has obvious poisoning symptom, do not have dead the generation.Cut open inspection, morphological changes of various tissue components such as each main organs macroscopy is big in not seeing obviously, hyperemia, necrosis, the weight coefficient of the heart, liver, spleen, lung, kidney, thymus, adrenal gland, uterus, testis is compared with matched group, no significant difference, histopathologic examination there is no unusual.Illustrate that mastitis one spray spirit is safer, in institute's amount of reagent scope, do not have obvious toxic-side effects.(raw data is kept in company's institute of materia medica's reference room)
2.6, the clever stability test of mastitis one spray
For investigating this product to time dependent rule under temperature, humidity, the light influence, for production, packing, transportation and the application of medicine provides scientific basis, formulate the effect duration of medicine simultaneously by experimental study, carried out the accelerated stability test and the long-term stable experiment of this product with reference to " Chinese veterinary drug allusion quotation ".
2.6.1, accelerated test
Get three batches of this product, sample number is that 050501,050502,050503 usefulness vinyon bottle is packed, and places ten days under illumination 4500 ± 500Lx condition, respectively at regularly sampling in five, ten days, character, container tightness is detected, and the results are shown in Table 7.
Table 7 mastitis one spray halo accelerated test measurement result
2.6.2, the temperature, the humidity accelerated test
Getting three batches of this product, press commercially available back, is that 30 ± 2 ℃, relative humidity are to place 6 months under 60 ± 5% the condition in temperature, respectively at sampling at 0,1,2,3,4,5,6 the end of month once, character, container tightness project is detected, and the results are shown in Table 8.
Table 8 mastitis one spray spirit temperature, humidity accelerated tests result
2.6.3, long term test
Long term test is to carry out under the actual storage requirement near medicine, its objective is that the effect duration for working out medicine provides foundation.Get three batches of this product, sample number is 050501,050502,050503, is that 25 ℃ ± 2 ℃, relative humidity are to place under 60 ± 5% the condition by commercially available back in temperature, respectively at taking a sample for 0,3,6,9,12,18,24,36 the end of month, character, container tightness are detected, the results are shown in Table 9.
The clever long-term stable experiment result of table 9 mastitis one spray
According to above-mentioned result of the test, tentatively draw to draw a conclusion:
Accelerated test result shows: this product is used the fill of vinyon bottle, places ten days under illumination 4500 ± 500Lx condition, respectively at regularly sampling detection in five, ten days; In temperature is that 30 ± 2 ℃, relative humidity are to place 6 months under 60 ± 5% the condition, and the end of each month, sampling detected, respectively with 0 month relatively, steady quality.
Long-term test results shows: this product is that 25 ± 2 ℃, relative humidity are to place 36 months under 60 ± 5% the condition in temperature, after testing, with 0 month relatively, every detection index has no significant change steady quality.
According to preliminarily stabilised Journal of Sex Research result, this product adopts the inner packaging material of vinyon bottle as product, and sealing is preserved, and medicine can obtain good stable, but because its solvent is an ethanol, so should put the moon place of drying in the air.This product holding conditions is preserved for sealing, put shady and cool place.This product effect duration is tentative to be 3 years.
2.7, the embodiment and the quality standard of mastitis of the present invention spray spirit
2.7.1, embodiment
Embodiment 1: be used for the treatment of the Chinese herbal medicine spray of milking-stock mastitis, the raw materials of effective components medicine and the percentage by weight thereof that make this medicine are: Olibanum 20%, Myrrha 20%, Flos Carthami 20%, Semen Persicae 20%, Folium et Cacumen Artemisiae Halodendri 20%.
Preparation method: concentrated solution 1: get Olibanum, Myrrha, Flos Carthami and Semen Persicae raw material in the prescription, use ethanol extraction, filtrate is concentrated into every milliliter and contains 2 gram crude drugs; Concentrated solution 2: get the Folium et Cacumen Artemisiae Halodendri raw material in the prescription, use ethanol extraction, filtrate is concentrated into every milliliter and contains 2 gram crude drugs; Then according to concentrated solution 1: concentrated solution 2: propylene glycol=ratio cooperated in 4: 1: 1.2, added ethanol to total amount 1g crude drug/mL, and mix homogeneously is sub-packed in automiser spray promptly.
Embodiment 2: be used for the treatment of the Chinese herbal medicine spray of milking-stock mastitis, the raw materials of effective components medicine and the percentage by weight thereof that make this medicine are: Olibanum 25%, Myrrha 25%, Flos Carthami 20%, Semen Persicae 20%, Folium et Cacumen Artemisiae Halodendri 10%.
Embodiment 3: be used for the treatment of the Chinese herbal medicine spray of milking-stock mastitis, the raw materials of effective components medicine and the percentage by weight thereof that make this medicine are: Olibanum 20%, Myrrha 20%, Flos Carthami 25%, Semen Persicae 25%, Folium et Cacumen Artemisiae Halodendri 10%.
Embodiment 4: be used for the treatment of the Chinese herbal medicine spray of milking-stock mastitis, the raw materials of effective components medicine and the percentage by weight thereof that make this medicine are: Olibanum 22%, Myrrha 23%, Flos Carthami 22%, Semen Persicae 20%, Folium et Cacumen Artemisiae Halodendri 13%.
Embodiment 5: Olibanum 20%, Myrrha 22%, Flos Carthami 24%, Semen Persicae 23%, Folium et Cacumen Artemisiae Halodendri 11%.
The preparation method of embodiment 2,3,4,5 is with embodiment 1.
2.7.2, discrimination method
Flos Carthami is got this product stock solution as need testing solution.Other gets Flos Carthami control medicinal material 0.5g, adds 80% acetone 5ml, close plug, jolting 15min, leave standstill, draw supernatant, draw each solution 5 μ l, put respectively on same silica gel H lamellae, (7: 2: 4: 0.4) be developing solvent, expansion was taken out, and dries with ethyl acetate-formic acid-water-methanol.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show identical purple principal spot.
Olibanum is got this product stock solution as need testing solution.Other gets Olibanum control medicinal material 0.4g, adds 60% ethanol 10ml, and ultrasonic 10min filters, and filtrate is medical material solution in contrast.Drawing each solution 3 μ l, put respectively on same silica gel g thin-layer plate, is developing solvent with normal hexane-ethyl acetate (17: 3), launches, and takes out, and dries.Spray is with 5% vanillin sulfuric acid solution, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show identical purple principal spot.
Myrrha is got this product stock solution as need testing solution.Other gets Myrrha control medicinal material 0.4g, adds 60% ethanol 10ml, and ultrasonic 10min filters, and filtrate is medical material solution in contrast.Drawing each 5 μ l of above-mentioned 3 kinds of solution, put respectively on same silica gel g thin-layer plate, is developing solvent with normal hexane-acetic acid second intoxicated (16: 2), launches, and takes out, and dries, and spray is with 5% vanillin sulfuric acid solution.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on show the speckle of same color.
2.8, the clever function of mastitis one spray cures mainly and using method and storage
2.8.1, function and curing mainly
Antibacterial and detoxicating, dispersing swelling and dissipating binds, blood circulation promoting and blood stasis dispelling, putrefaction-removing granulation-promoting, the sharp breast of collateral dredging.Be used for the treatment of and prevent various mammitis of animal such as milch cow, milk goat, horse, pig.
2.8.2, be suitable for symptom
The early stage milk character of various domestic animal clinic mastitis is unusual, thin, includes ziega or floccule, and what have is mixed with blood or pus, and the milk that has is discharged not smooth, and lactation amount reduces or stops.The middle and late stage breast is red, swollen, hot, bitterly, the lymphonodi mammarici enlargement.Simultaneously with General Symptomies such as lassitude, appetite minimizing, fervescence.
The general clinical symptoms of latent mammitis is not obvious, and sensation checks that milk does not have ANOMALOUS VARIATIONS, only shows milk yield and reduces gradually, and most alcohol testings are positive.
2.8.3 using method
Every bottle of 100mL～200mL of mastitis one spray spirit with before shaking up, aims at and suffers from poultry breast even spraying, each 15mL～20mL, every day 2 times, logotype 5～6 days.(using one bottle each course of treatment)
2.8.4, the storage
Seal, put the storage of shady and cool place.
If be used for producing in batches, can amplify by above-mentioned dose ratio.
The present invention is used in combination with " Chinese veterinary drug allusion quotation " (2005 editions) GONGYINGSAN, and then curative effect is better.Its prescription is as follows: Herba Taraxaci 60g, Flos Lonicerae 60g, Fructus Forsythiae 60g, Retinervus Luffae Fructus 30g, Medulla Tetrapanacis 25g, Folium Hibisci Mutabilis 25g, Bulbus Fritillariae Thunbergii 30g.
3, the concrete case of mastitis one spray spirit
Case 1:2007 May 23, the ancient Wei Zhen raiser king of Shanxi Province's Ruicheng County * * one 5 years old Fresian, main suit: find both sides breast redness when milking before 3 days, be mixed with blood in the milk, lassitude, appetite reduces, please local veterinary inspection, be diagnosed as acute mastitis, use penicillin, streptomycin, chain jamming mycin etc. ineffective, check, this milch cow lassitude, appetite reduces, and ruminates minimizing, 39.5 ℃ of body temperature, 70 times/min of heart beating, breathe 50 times/min, bilateral breast red and swollen heat pain is obvious, the lymphonodi supramammarici enlargement.During treatment, the local scrub of breast, with mastitis one spray spirit, the 15mL～20mL that at every turn sprays, 4 times/day, that the swelling has gone down is more than half for breast during the next day further consultation, one spray spirit every day of reuse mastitis 2 times, uses sick cattle recovery from illness continuously 5 days.
Case 2:2006 March 3, Shaanxi Province's Weinan City Linwei District Cao one cow head is examined the main suit: milk production of cow descends to some extent recently, the milk sense organ is normal, but rejects behind the milking station alcohol testing, further checks, milk acidity is 20, alcohol positive milk, high acidity, be diagnosed as latent mammitis, use mastitis one spray spirit, each 15mL～20mL, 2 times/day, logotype 3 days, the next day after, milk acceptance(check), milk production of cow also have rise.
Case 3:2007 December 5 days, acute mastitis takes place in 10 milk in milch cow sub-district, Tangshan City, Hebei province, uses this medicine by case 1 method, and the other 18 cow head milk alcohol detection positives are used this medicine by case 2 methods, all recovery from illness.
Claims (2)
1. Chinese herbal medicine spray that is used for the treatment of milking-stock mastitis, it is characterized in that the raw materials of effective components medicine and the percentage by weight thereof that make this Chinese herbal medicine spray are: Olibanum 20%～25%, Myrrha 20%～25%, Flos Carthami 20%～25%, Semen Persicae 20%～25%, Folium et Cacumen Artemisiae Halodendri 10%～20%; The percentage by weight sum of above-mentioned raw materials medicine is 100%.
2. one kind prepares the method that is used for the treatment of the Chinese herbal medicine spray of milking-stock mastitis as claimed in claim 1, it is characterized in that step is as follows:
1), get Olibanum, Myrrha, Flos Carthami and Semen Persicae crude drug in the prescription, use ethanol extraction, filtrate be concentrated into every milliliter contain 2 the gram crude drugs, concentrated solution 1;
2), get the Folium et Cacumen Artemisiae Halodendri crude drug in the prescription, use ethanol extraction, filtrate be concentrated into every milliliter contain 2 the gram crude drugs, concentrated solution 2;
3), the concentrated solution of above-mentioned preparation is cooperated concentrated solution 1 according to following ratio: concentrated solution 2: propylene glycol=4: 1: 1.2, add ethanol to total amount 1g crude drug/mL, mix homogeneously is sub-packed in automiser spray promptly.
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| CN107823375A (en) * | 2017-11-27 | 2018-03-23 | 青岛农业大学 | A kind of Chinese medicinal tablet for treating mastitis for milk cows and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1660274A (en) * | 2004-12-29 | 2005-08-31 | 李发明 | Combination of lmedication for treatig mastitis of cow and preparation method |
| CN101406609A (en) * | 2008-11-18 | 2009-04-15 | 布焕景 | External-use medicament for treating breast inflammation of milk cattle |
| CN101417045A (en) * | 2008-10-31 | 2009-04-29 | 天津必佳药业集团有限公司 | Traditional Chinese medicine composition for treating cow mastitis |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1660274A (en) * | 2004-12-29 | 2005-08-31 | 李发明 | Combination of lmedication for treatig mastitis of cow and preparation method |
| CN101417045A (en) * | 2008-10-31 | 2009-04-29 | 天津必佳药业集团有限公司 | Traditional Chinese medicine composition for treating cow mastitis |
| CN101406609A (en) * | 2008-11-18 | 2009-04-15 | 布焕景 | External-use medicament for treating breast inflammation of milk cattle |
Non-Patent Citations (1)
| Title |
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| 刘瑞生 等."中草药防治奶牛乳房炎研究进展".《中国动物保健》.2008,(第118期), |
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Effective date of registration: 20160602 Address after: 044600 north of Industrial Road, Ruicheng County, Yuncheng County, Shanxi Province Patentee after: SHANXI ZHAOYI BIOLOGICAL CO., LTD. Address before: 044600 Yongle East Street, Ruicheng County, Shanxi Patentee before: Shanxi Ruicheng Yabaoshouyao Co., Ltd. |