CN104382883A - Preparation method for nanometer drug-loaded fiber membrane with temperature-sensitive drug release performance - Google Patents
Preparation method for nanometer drug-loaded fiber membrane with temperature-sensitive drug release performance Download PDFInfo
- Publication number
- CN104382883A CN104382883A CN201410431591.8A CN201410431591A CN104382883A CN 104382883 A CN104382883 A CN 104382883A CN 201410431591 A CN201410431591 A CN 201410431591A CN 104382883 A CN104382883 A CN 104382883A
- Authority
- CN
- China
- Prior art keywords
- preparation
- fibrous membrane
- release performance
- drug
- thermo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
A related preparation method for a nanometer drug-loaded fiber membrane with temperature-sensitive drug release performance comprises: (1) preparing a spinning solution containing a pain-easing medicine ketoprofen; and (2) preparing the nanometer fiber membrane from the above obtained spinning solution through an electrostatic spinning process, and performing vacuum drying to obtain a temperature-sensitive drug-loaded nanometer analgesic. The nanometer analgesic is capable of rapidly enduringly easing pain, is easy to carry and convenient for administration. The preparation method is simple, free of pollution, low in cost and free of special requirements on equipment, and is suitable for large-scale production.
Description
Technical field
The invention belongs to nano drug-carrying fibrous membrane preparation field, particularly a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance.
Background technology
Nano fibrous membrane has the large and porosity high of specific surface area, can be applicable to the fields such as isolated by filtration, bio-sensing and organizational project..Electrostatic spinning, as a kind of easy, quick, general nanofiber technology of preparing, receives the extensive concern of Chinese scholars in recent years.Along with the development of electrostatic spinning technique, the morphosis of Electrospun nano-fibers is day by day diversified, and single nanofiber, height-oriented nanofiber and the nanofiber with special layers aggregated(particle) structure or morphological characteristic are all seen in report.And method of electrostatic spinning is prepared nanofiber and is applicable to numerous polymer, even comprise biomacromolecule, inorganic particulate and some small-molecule substance.
Conventional medicament and preparation exist in clinical practice that drug effectiveness is low in vivo mostly, toxic and side effects and need frequent medication to maintain the shortcomings such as drug effect.Drug controlled release technology is exactly that medicine or other active substance and suitable carrier are made preparation by certain form, controls the process of medicine in people's body absorption, metabolism and excretion.Make medicine by the dosage of design, discharge in vivo with certain pattern in the time range required or make medicine reach the object of certain disease for the treatment of in appointed part release, compared with convenient administration mode, based Controlled-release Drug not only can reduce administration number of times, maintain the concentration of blood Chinese medicine, thus solve the problem of drug level instability, but also, reduce drug toxicity, improve the curative effect of medicine.
In order to find suitable pharmaceutical carrier, people to various system as microsphere, liposome, microemulsion etc. are studied.These aggregations such as micelle, microemulsion, gel, liquid crystal, vesicle have the ability that bag carries drug molecule, have good permeability again simultaneously, become the important research field of pharmaceutical carrier to film.In recent years, along with the development and application of electrostatic spinning technique, many researcheres adopt biodegradable synthesized polymer material to prepare electrostatic spinning nano fiber for pharmaceutical carrier, and study its medicine-releasing performance.The specific surface area of nanofiber is large, utilizes it as medicine carrying material, and the drug slow that some can be made originally to be difficult to be absorbed by the body decomposes release, to reach therapeutic effect.
Temperature response type delivery systme can carry out regulating drug by external heating or cooling and discharge, and also directly can discharge medicine by responding to body temperature.The block copolymer (PNIPAM-b-PBMA) of poly-N-isopropyl Bing Xi Ugly amine and butyl methacrylate such as Chung forms nanoparticle parcel amycin (ADR) in water, when temperature is under its LCST, the hydrophilic PNIPAM chain of particle shell plays Stabilization to particle on the one hand, stops particle and cells contacting to discharge medicine on the other hand.When particle temperature is higher than LCST, PNIPAM shell is collapsed and is become hydrophobicity, and particle and cells contacting also discharge medicine.
Because the change of temperature easily realizes, and can not change the chemical composition of system, temperature response type delivery systme becomes the maximum class stimuli responsive type delivery systme of current research.
Summary of the invention
The present invention relates to a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance, fibrous membrane of the present invention can realize the controllable release of medicine, and nano-analgesic agent of the present invention can ease pain fast, lastingly, is easy to carry about with one, convenient drug administration; Preparation method of the present invention is simple, and pollution-free, cost is low, to equipment without particular/special requirement, can be mass-produced.
A kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance of the present invention, comprising:
(1) take poly-N-isopropyl acrylamide PNIPAAm, ethyl cellulose EC respectively, dissolve with the mixture of dehydrated alcohol by them, then add medicine in the solution obtained; By last gained solution magnetic agitation 24-48h, until dissolve completely, obtain spinning solution; Wherein, the mass ratio of PNIPAAm and EC is 1: 1-4;
(2) spinning solution of above-mentioned gained is prepared nano fibrous membrane by method of electrostatic spinning, the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance after vacuum drying, can be obtained.
The solvent dissolving PNIPAAm and EC mixture in step (1) is dehydrated alcohol.
Described in step (1), the concentration of PNIPAAm and EC mixed solution is 25% grams per milliliter.
Described in step (1), the mass ratio of PNIPAAm and EC is 1: 2,1: 3 or 1: 4.
Medicine described in step (1) is non-steroid antiinflammatory drug NSAIDs, and described non-steroid antiinflammatory drug is ketoprofen KET.
The mass percent that medicine described in step (1) accounts for mixed polymer (PNIPAAm and EC) is 25%.
The process conditions of electrostatic spinning described in step (2) are voltage 10-15 kilovolt, and receiver sheet is from spinning nozzle distance 120-200 millimeter, and syringe fltting speed is 0.1-1.0 ml/hour.
beneficial effect
(1) fibrous membrane of the present invention can realize the temperature sensitive controllable release of medicine, has fast, function of easing pain lastingly, is easy to carry about with one, convenient drug administration;
(2) preparation method of the present invention is simple, and cost is low, to equipment without particular/special requirement, can be mass-produced.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph (PNIPAAm and EC mass ratio is 1: 2) of nano drug-carrying fibrous membrane;
Fig. 2 is the stereoscan photograph (PNIPAAm and EC mass ratio is 1:3) of nano drug-carrying fibrous membrane;
Fig. 3 is nano drug-carrying fibrous membrane Dissolution profiles.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
(1) spinning solution is prepared: take 0.167gPNIPAAm and 0.333gEC respectively with electronic analytical balance, the mixture of the two is dissolved in 2 milliliters of dehydrated alcohol, 0.125gKET is added again in the solution obtained, last gained solution room temperature lower magnetic force is stirred 24-48h, until dissolve (in solution, the mass ratio of PNIPAAm and EC is 1: 2) completely.
(2) above-mentioned gained electrospinning liquid is carried out spinning on electrostatic spinning apparatus, select No. 9 syringe needles, wherein voltage is 13 kilovolts, syringe fltting speed is 0.5 ml/hour, receiver sheet is 15 centimetres from spinning nozzle distance, ambient temperature is (25 ± 1) DEG C, and ambient humidity is 57 ± 3%.Receive with aluminium-foil paper, in device to be injected after spinning liquid electrospinning, take off fibrous membrane, vacuum drying 24 hours under room temperature.
Embodiment 2
(1) spinning solution is prepared: take 0.125gPNIPAAm and 0.375gEC respectively with electronic analytical balance, the mixture of the two is dissolved in 2 milliliters of dehydrated alcohol, 0.125gKET is added again in the solution obtained, last gained solution room temperature lower magnetic force is stirred 24-48h, until dissolve (in solution, the mass ratio of PNIPAAm and EC is 1: 3) completely.
(2) above-mentioned gained electrospinning liquid is carried out spinning on electrostatic spinning apparatus, select No. 9 syringe needles, wherein voltage is 13 kilovolts, syringe fltting speed is 0.5 ml/hour, receiver sheet is 15 centimetres from spinning nozzle distance, ambient temperature is (25 ± 1) DEG C, and ambient humidity is 57 ± 3%.Receive with aluminium-foil paper, in device to be injected after spinning liquid electrospinning, take off fibrous membrane, vacuum drying 24 hours under room temperature.
Embodiment 3
(1) spinning solution is prepared: take 0.100gPNIPAAm and 0.400gEC respectively with electronic analytical balance, the mixture of the two is dissolved in 2 milliliters of dehydrated alcohol, 0.125gKET is added again in the solution obtained, last gained solution room temperature lower magnetic force is stirred 24-48h, until dissolve (in solution, the mass ratio of PNIPAAm and EC is 1: 4) completely.
(2) above-mentioned gained electrospinning liquid is carried out spinning on electrostatic spinning apparatus, select No. 9 syringe needles, wherein voltage is 13 kilovolts, syringe fltting speed is 0.5 ml/hour, receiver sheet is 15 centimetres from spinning nozzle distance, ambient temperature is (25 ± 1) DEG C, and ambient humidity is 57 ± 3%.Receive with aluminium-foil paper, in device to be injected after spinning liquid electrospinning, take off fibrous membrane, vacuum drying 24 hours under room temperature.
Claims (8)
1. there is a preparation method for the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance, comprise the steps:
(1) take poly-N-isopropyl acrylamide PNIPAAm, ethyl cellulose EC respectively, dissolve with the mixture of dehydrated alcohol by them, then add medicine in the solution obtained; By last gained solution magnetic agitation 24-48h, until dissolve completely, obtain spinning solution; Wherein, the mass ratio of PNIPAAm and EC is 1: 1-4;
(2) spinning solution of above-mentioned gained is prepared nano fibrous membrane by method of electrostatic spinning, the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance after vacuum drying, can be obtained.
2. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 1, is characterized in that:
The solvent dissolving PNIPAAm and EC mixture in step (1) is dehydrated alcohol.
3. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 1, is characterized in that:
Described in step (1), the concentration of PNIPAAm and EC mixed solution is 25% grams per milliliter.
4. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 1, is characterized in that:
Described in step (1), the mass ratio of PNIPAAm and EC is 1: 2,1: 3 or 1: 4.
5. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 1, is characterized in that:
Medicine described in step (1) is non-steroid antiinflammatory drug NSAIDs.
6. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 5, is characterized in that:
Described non-steroid antiinflammatory drug is ketoprofen KET.
7. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 1, is characterized in that:
The mass percent that medicine described in step (1) accounts for polymer P NIPAAm and EC is 25%.
8. a kind of preparation method with the nano drug-carrying fibrous membrane of Thermo-sensitive Release Performance as claimed in claim 1, is characterized in that:
The process conditions of electrostatic spinning described in step (2) are voltage 10-15 kilovolt, and receiver sheet is from spinning nozzle distance 120-200 millimeter, and syringe fltting speed is 0.1-1.0 ml/hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410431591.8A CN104382883B (en) | 2014-08-28 | 2014-08-28 | A kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410431591.8A CN104382883B (en) | 2014-08-28 | 2014-08-28 | A kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104382883A true CN104382883A (en) | 2015-03-04 |
| CN104382883B CN104382883B (en) | 2017-06-30 |
Family
ID=52600914
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410431591.8A Expired - Fee Related CN104382883B (en) | 2014-08-28 | 2014-08-28 | A kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104382883B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928851A (en) * | 2015-06-24 | 2015-09-23 | 东华大学 | Preparation method for silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane |
| CN105926080A (en) * | 2016-06-14 | 2016-09-07 | 东华大学 | Preparation method of thermo-sensitive PNIPAAm (ploy(N-isopropylacrylamide)/PVP (polyvinyl pyrrolidone) composite fibers |
| CN106048902A (en) * | 2016-07-15 | 2016-10-26 | 东华大学 | Ethyl cellulose drug-loading nanofiber membrane and preparation method and application thereof |
| CN107419432A (en) * | 2017-08-03 | 2017-12-01 | 东华大学 | A kind of sensitive medicament-carrying nano-fiber membrane and its preparation method and application |
| CN107447366A (en) * | 2017-08-03 | 2017-12-08 | 东华大学 | A kind of sensitive medicament-carried sustained release nano fiber films of pH and its preparation method and application |
| CN107536827A (en) * | 2017-08-03 | 2018-01-05 | 东华大学 | A kind of sensitive medicament-carried sustained release nano fiber film of temperature and its preparation method and application |
| CN111945415A (en) * | 2020-07-10 | 2020-11-17 | 东华大学 | Drug-loaded thermochromic hydrogel functionalized fabric and preparation and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865104A (en) * | 2014-02-21 | 2014-06-18 | 东华大学 | Preparation method of core-shell nano-particles |
-
2014
- 2014-08-28 CN CN201410431591.8A patent/CN104382883B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103865104A (en) * | 2014-02-21 | 2014-06-18 | 东华大学 | Preparation method of core-shell nano-particles |
Non-Patent Citations (2)
| Title |
|---|
| XIULING LIN ET AL: ""Controllable drug release of electrospun thermoresponsive poly(N-isopropylacrylamide)/poly(2-acrylamido-2- methylpropanesulfonic acid) nanofibers"", 《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH》 * |
| 李山山等: ""静电纺丝的研究进展"", 《合成纤维工业》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104928851A (en) * | 2015-06-24 | 2015-09-23 | 东华大学 | Preparation method for silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane |
| CN105926080A (en) * | 2016-06-14 | 2016-09-07 | 东华大学 | Preparation method of thermo-sensitive PNIPAAm (ploy(N-isopropylacrylamide)/PVP (polyvinyl pyrrolidone) composite fibers |
| CN106048902A (en) * | 2016-07-15 | 2016-10-26 | 东华大学 | Ethyl cellulose drug-loading nanofiber membrane and preparation method and application thereof |
| CN107419432A (en) * | 2017-08-03 | 2017-12-01 | 东华大学 | A kind of sensitive medicament-carrying nano-fiber membrane and its preparation method and application |
| CN107447366A (en) * | 2017-08-03 | 2017-12-08 | 东华大学 | A kind of sensitive medicament-carried sustained release nano fiber films of pH and its preparation method and application |
| CN107536827A (en) * | 2017-08-03 | 2018-01-05 | 东华大学 | A kind of sensitive medicament-carried sustained release nano fiber film of temperature and its preparation method and application |
| CN111945415A (en) * | 2020-07-10 | 2020-11-17 | 东华大学 | Drug-loaded thermochromic hydrogel functionalized fabric and preparation and application thereof |
| CN111945415B (en) * | 2020-07-10 | 2021-10-26 | 东华大学 | Drug-loaded thermochromic hydrogel functionalized fabric and preparation and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104382883B (en) | 2017-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104382883A (en) | Preparation method for nanometer drug-loaded fiber membrane with temperature-sensitive drug release performance | |
| Song et al. | Novel RGD containing, temozolomide-loading nanostructured lipid carriers for glioblastoma multiforme chemotherapy | |
| Tavano et al. | Doxorubicin loaded magneto-niosomes for targeted drug delivery | |
| Liu et al. | Preparation of a ligustrazine ethosome patch and its evaluation in vitro and in vivo | |
| CN103239730B (en) | Medical sodium alginate gel microsphere and preparation method and application thereof | |
| CN103990133B (en) | A kind of mesoporous carbon nanoparticle system and application thereof with targeting positioning release medicine | |
| CN102824641A (en) | Two-phase drug-release multilayer drug-loaded nanofiber mat and preparation method thereof | |
| Ge et al. | Electrospun self-emulsifying core-shell nanofibers for effective delivery of paclitaxel | |
| CN108295045B (en) | Liquid crystal gel microcapsule and preparation method thereof | |
| CN106821963A (en) | A kind of method of utilization cellulose base temperature and the load of pH sensitive hydrogels and slow releasing pharmaceutical | |
| CN102772802A (en) | Oleanolic acid nanoliposome modified by chitosan and polyethylene glycol and preparation method thereof | |
| Wang et al. | Synthesis, antimicrobial and release of chloroamphenicol loaded poly (l-lactic acid)/ZrO2 nanofibrous membranes | |
| CN102406609A (en) | Lidocaine hydrochloride polymeric liposome for topical anesthesia and preparation method | |
| Vidal-Romero et al. | Development and characterization of pH-dependent cellulose acetate phthalate nanofibers by electrospinning technique | |
| CN104264372B (en) | Method for preparing PNIPAAm (poly(N-isopropylacrylamide)) and EC (ethyecellulose) blended nano-fiber membrane by electrostatic spinning | |
| CN105838013A (en) | pH sensitive composite nano gel based on methyl vinyl ether maleic acid copolymer and chitosan and preparation method thereof | |
| CN101088505B (en) | Nanometer breviscapine polymer particle preparation and its preparation process | |
| Zhu et al. | Enhanced oral bioavailability of capsaicin‐loaded microencapsulation complex via electrospray technology: Preparation, in vitro and in vivo evaluation | |
| CN104490844A (en) | Core-shell-type microgel and preparation method thereof | |
| CN103211758A (en) | Liquid crystal nanoparticle transdermal agent and preparation method thereof | |
| CN107496936A (en) | A kind of both sexes small molecule self assembly targeted nanoparticles drug-loading system and preparation method thereof | |
| CN106512013A (en) | Drug-loaded composite nanofiber membrane used for drug release, and making method thereof | |
| CN106361727A (en) | Bilobalide-PVA nanoparticle and preparation method thereof | |
| CN103193998B (en) | Preparation method of chitosan-palygorskite-polyving akohol ternary composite medicine sustained-release diaphragm | |
| CN101278897B (en) | Process for preparing brevifolin solid lipid nano particle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170630 Termination date: 20200828 |