CN104382883B - A kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance - Google Patents
A kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance Download PDFInfo
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- CN104382883B CN104382883B CN201410431591.8A CN201410431591A CN104382883B CN 104382883 B CN104382883 B CN 104382883B CN 201410431591 A CN201410431591 A CN 201410431591A CN 104382883 B CN104382883 B CN 104382883B
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Abstract
The present invention relates to a kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance, including：(1) spinning solution containing analgesic Ketoprofen is prepared；(2) spinning solution of above-mentioned gained is prepared into nano fibrous membrane by method of electrostatic spinning, Thermo-sensitive medicament-carried nano analgestic is can obtain after vacuum drying.Nano-analgesic agent of the invention can quick, persistently analgesia, it is easy to carry, convenient drug administration；Preparation method of the invention is simple, and pollution-free, low cost, to equipment without particular/special requirement, can be mass-produced.
Description
Technical field
The invention belongs to nano drug-carrying fiber field of membrane preparation, more particularly to a kind of nanometer with Thermo-sensitive Release Performance
The preparation method of drug-loading fibre film.
Background technology
Nano fibrous membrane has specific surface area big and the features such as porosity is high, can be applied to be separated by filtration, bio-sensing and
The field such as organizational project.Electrostatic spinning is received in recent years as a kind of easy, quick, general nanofiber technology of preparing
The extensive concern of domestic and foreign scholars.With continuing to develop for electrostatic spinning technique, the morphosis day of Electrospun nano-fibers
Beneficial variation, single nanofiber, height-oriented nanofiber and the Nanowire with special hierarchical structure or morphological feature
Dimension is all seen in report.And, method of electrostatic spinning prepares nanofiber suitable for numerous polymer, in addition including large biological molecule,
Inorganic particulate and some small-molecule substances.
There is low drug effectiveness, toxic and side effect mostly in vivo in clinical practice and need frequent in conventional medicament and preparation
The shortcomings of medication is to maintain drug effect.Drug controlled release technology be exactly by medicine or other active material and appropriate carrier by
Certain form is made preparation, process of the control medicine in people's body absorption, metabolism and excretion.Make medicine by the agent of design
Amount, discharged in vivo with certain pattern in desired time range or made medicine appointed part discharge and reach treatment certain
The purpose of disease is planted, compared with convenient administration mode, based Controlled-release Drug can not only reduce administration number of times, maintain medicine in blood
Concentration, so as to solve the problems, such as that drug concentration is unstable, but also, reduce drug toxicity, improve the curative effect of medicine.
In order to find suitable pharmaceutical carrier, people are ground to various systems such as microballoon, liposome, microemulsion etc.
Study carefully.These aggregations such as micella, microemulsion, gel, liquid crystal, vesica have the ability for containing drug molecule, while have to film again
Good permeability, the important research field as pharmaceutical carrier.In recent years, with the development and application of electrostatic spinning technique,
Many researcher Bian prepare electrostatic spinning nano fiber for pharmaceutical carrier with biodegradable synthesis macromolecular material, and study
Its medicine-releasing performance.The specific surface area of nanofiber is big, utilizes it as medicine carrying material, and some can be made to be difficult to originally by people
The medicine that body absorbs slowly decomposes release, to reach therapeutic effect.
Temperature response type delivery systme can carry out regulating drug and discharge by heating in vitro or lowering the temperature, also can be directly by body
Temperature responds to discharge medicine.Poly-N-isopropyl Bing Xi Ugly amine and the block copolymer of butyl methacrylate such as Chung
(PNIPAM-b-PBMA) nano-particle is formed in water and wraps up adriamycin (ADR), when temperature is under its LCST, outside particle
On the one hand the hydrophilic PNIPAM chains of shell play stabilization to particle, on the other hand prevent particle from discharging medicine with cells contacting.When
When particle temperature is higher than LCST, PNIPAM shells are collapsed and are changed into hydrophobicity, and particle and cells contacting simultaneously discharge medicine.
Because the change of temperature is easily realized, and the chemical composition of system will not be changed, temperature response type delivery systme into
It is the most class stimuli responsive type delivery systme of current research.
The content of the invention
The present invention relates to a kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance, fibre of the invention
Dimension film can realize the controlled release of medicine, and nano-analgesic agent of the invention can quick, persistently analgesia, it is easy to carry, to prescription
Just；Preparation method of the invention is simple, and pollution-free, low cost, to equipment without particular/special requirement, can be mass-produced.
A kind of preparation method of nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance of the invention, including：
(1) poly-N-isopropyl acrylamide PNIPAAm, ethyl cellulose EC are weighed respectively, with absolute ethyl alcohol by they
Mixture is dissolved, then to adding medicine in the solution for obtaining；By last resulting solution magnetic agitation 24-48h, until completely
Dissolving, obtains spinning solution；Wherein, the mass ratio of PNIPAAm and EC is 1：1-4；
(2) spinning solution of above-mentioned gained is prepared into nano fibrous membrane by method of electrostatic spinning, is can obtain after vacuum drying
The nano drug-carrying tunica fibrosa of Thermo-sensitive Release Performance.
Dissolving PNIPAAm and the solvent of EC mixtures are absolute ethyl alcohol in step (1).
PNIPAAm described in step (1) is 25% grams per milliliter with the concentration of EC mixed solutions.
The mass ratio of PNIPAAm described in step (1) and EC is 1:2、1:3 or 1:4.
Medicine described in step (1) is nonsteroidal anti-inflammatory drug NSAIDs, and the nonsteroidal anti-inflammatory drug is Ketoprofen KET.
The mass percent that medicine described in step (1) accounts for mixed polymer (PNIPAAm and EC) is 25%.
The process conditions of electrostatic spinning described in step (2) are voltage 10-15 kilovolts, and receiver sheet is from spinning nozzle distance 120-
200 millimeters, syringe fltting speed is 0.1-1.0 mls/hour.
Beneficial effect
(1) tunica fibrosa of the invention can realize the temperature sensitive controlled release of medicine, with quick, function of persistently easing pain, easily
In carrying, convenient drug administration；
(2) preparation method of the invention is simple, and low cost, to equipment without particular/special requirement, can be mass-produced.
Brief description of the drawings
Fig. 1 is that (PNIPAAm and EC mass ratioes are 1 for the stereoscan photograph of nano drug-carrying tunica fibrosa：2)；
Fig. 2 is that (PNIPAAm and EC mass ratioes are 1 for the stereoscan photograph of nano drug-carrying tunica fibrosa：3)；
Fig. 3 is nano drug-carrying tunica fibrosa Dissolution profiles.
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.In addition, it is to be understood that after the content for having read instruction of the present invention, people in the art
Member can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Scope.
Embodiment 1
(1) spinning solution is prepared：Weigh 0.167gPNIPAAm and 0.333gEC respectively with electronic analytical balance, incite somebody to action the two
Mixture be dissolved in 2 milliliters of absolute ethyl alcohols, then to 0.125gKET is added in the solution for obtaining, by last resulting solution normal temperature
Lower magnetic agitation 24-48h, until being completely dissolved, (mass ratio of PNIPAAm and EC is 1 in solution：2).
(2) above-mentioned gained electrospinning liquid is carried out into spinning on electrostatic spinning apparatus, from No. 9 syringe needles, wherein voltage is 13
Kilovolt, syringe fltting speed be 0.5 ml/hour, receiver sheet from spinning nozzle with a distance from be 15 centimetres, environment temperature be (25 ±
1) DEG C, ambient humidity is 57 ± 3%.Received with aluminium-foil paper, after spinning solution electrospinning is finished in device to be injected, removed fiber
Film, is vacuum dried 24 hours under normal temperature.
Embodiment 2
(1) spinning solution is prepared：Weigh 0.125gPNIPAAm and 0.375gEC respectively with electronic analytical balance, incite somebody to action the two
Mixture be dissolved in 2 milliliters of absolute ethyl alcohols, then to 0.125gKET is added in the solution for obtaining, by last resulting solution normal temperature
Lower magnetic agitation 24-48h, until being completely dissolved, (mass ratio of PNIPAAm and EC is 1 in solution：3).
(2) above-mentioned gained electrospinning liquid is carried out into spinning on electrostatic spinning apparatus, from No. 9 syringe needles, wherein voltage is 13
Kilovolt, syringe fltting speed be 0.5 ml/hour, receiver sheet from spinning nozzle with a distance from be 15 centimetres, environment temperature be (25 ±
1) DEG C, ambient humidity is 57 ± 3%.Received with aluminium-foil paper, after spinning solution electrospinning is finished in device to be injected, removed fiber
Film, is vacuum dried 24 hours under normal temperature.
Embodiment 3
(1) spinning solution is prepared：Weigh 0.100gPNIPAAm and 0.400gEC respectively with electronic analytical balance, incite somebody to action the two
Mixture be dissolved in 2 milliliters of absolute ethyl alcohols, then to 0.125gKET is added in the solution for obtaining, by last resulting solution normal temperature
Lower magnetic agitation 24-48h, until being completely dissolved, (mass ratio of PNIPAAm and EC is 1 in solution：4).
(2) above-mentioned gained electrospinning liquid is carried out into spinning on electrostatic spinning apparatus, from No. 9 syringe needles, wherein voltage is 13
Kilovolt, syringe fltting speed be 0.5 ml/hour, receiver sheet from spinning nozzle with a distance from be 15 centimetres, environment temperature be (25 ±
1) DEG C, ambient humidity is 57 ± 3%.Received with aluminium-foil paper, after spinning solution electrospinning is finished in device to be injected, removed fiber
Film, is vacuum dried 24 hours under normal temperature.
Claims (4)
1. a kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance, comprises the following steps：
(1) poly-N-isopropyl acrylamide PNIPAAm, ethyl cellulose EC are weighed respectively, with absolute ethyl alcohol by their mixing
Thing is dissolved, then to addition Ketoprofen KET in the solution for obtaining；By last resulting solution magnetic agitation 24-48h, until complete
CL, obtains spinning solution；Wherein, the mass ratio of PNIPAAm and EC is 1：1-4；The concentration of PNIPAAm and EC mixed solutions
It is 25% grams per milliliter；
(2) spinning solution of above-mentioned gained is prepared into nano fibrous membrane by method of electrostatic spinning, is can obtain after vacuum drying temperature sensitive
The nano drug-carrying tunica fibrosa of property Release Performance.
2. a kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance as claimed in claim 1, it is special
Levy and be：
The mass ratio of PNIPAAm described in step (1) and EC is 1:2、1:3 or 1:4.
3. a kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance as claimed in claim 1, it is special
Levy and be：
It is 25% that Ketoprofen KET described in step (1) accounts for polymer P NIPAAm with the gross mass percentage of EC mixtures.
4. a kind of preparation method of the nano drug-carrying tunica fibrosa with Thermo-sensitive Release Performance as claimed in claim 1, it is special
Levy and be：
The process conditions of electrostatic spinning described in step (2) are voltage 10-15 kilovolts, and receiver sheet is from spinning nozzle distance 120-200
Millimeter, syringe fltting speed is 0.1-1.0 mls/hour.
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| CN104928851B (en) * | 2015-06-24 | 2017-03-01 | 东华大学 | The preparation method of silver-carrying nano particle temperature stimulating responsive Hybrid nanofibers film |
| CN105926080A (en) * | 2016-06-14 | 2016-09-07 | 东华大学 | Preparation method of thermo-sensitive PNIPAAm (ploy(N-isopropylacrylamide)/PVP (polyvinyl pyrrolidone) composite fibers |
| CN106048902A (en) * | 2016-07-15 | 2016-10-26 | 东华大学 | Ethyl cellulose drug-loading nanofiber membrane and preparation method and application thereof |
| CN107536827A (en) * | 2017-08-03 | 2018-01-05 | 东华大学 | A kind of sensitive medicament-carried sustained release nano fiber film of temperature and its preparation method and application |
| CN107419432A (en) * | 2017-08-03 | 2017-12-01 | 东华大学 | A kind of sensitive medicament-carrying nano-fiber membrane and its preparation method and application |
| CN107447366A (en) * | 2017-08-03 | 2017-12-08 | 东华大学 | A kind of sensitive medicament-carried sustained release nano fiber films of pH and its preparation method and application |
| CN111945415B (en) * | 2020-07-10 | 2021-10-26 | 东华大学 | Drug-loaded thermochromic hydrogel functionalized fabric and preparation and application thereof |
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