CN104375268A - Optical structure capable of carrying out bright field scanning and fluorescence scanning - Google Patents
Optical structure capable of carrying out bright field scanning and fluorescence scanning Download PDFInfo
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- CN104375268A CN104375268A CN201410693154.3A CN201410693154A CN104375268A CN 104375268 A CN104375268 A CN 104375268A CN 201410693154 A CN201410693154 A CN 201410693154A CN 104375268 A CN104375268 A CN 104375268A
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- reflective mirror
- scanning
- fluorescence
- camera
- guide rail
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides an optical structure capable of carrying out bright field scanning and fluorescence scanning. The optical structure comprises a shell, a camera, a reflector I, a fluorescence camera, a fluorescence module, a reflector II, a guide rail and an objective, wherein the reflector II is located between the reflector I and the objective, the guide rail is connected with the reflector II, and the fluorescence camera, the fluorescence module and the reflector II are arranged in a straight line mode. The optical structure has the advantage of being capable of carrying out both bright field scanning and fluorescence scanning.
Description
Technical field
The present invention relates to a kind of optical scanning structure, particularly relate to a kind of digital pathological section scanning optical structure.
Background technology
Digital pathological section scanner day by day accept by Pathology Doctors ', digital pathology and the advantage that scanner brings at ordinary times is just being widely used in pathology medical industry.And digital pathological section scanner main object is common pathology section on market, fluorescent dye section can only rely on fluorescent microscope and carry out inspections and examinations.And microscopic examination section, instantaneity can not be had as digital pathological section scanner, can not remote diagnosis be realized.
Digital pathological section scanner can only be used for light field scanning, frame, imaging system, information extracting device is included as patent of invention ZL200620139251.9 discloses a kind of self-adapting slicing scanning system, X-axis transmission combination, Z-axis transmission combines, the transmission combination of section box.Foregoing invention patent only can carry out light field scanning.
Fluorescence section just can only can carry out inspections and examinations by fluorescent microscope, and the image seen can not be preserved, and diagnosis has instantaneity, and can not realize remote diagnosis, image can not be preserved for a long time, significantly limit medically diagnostic result fast and accurately.
Therefore, develop and a kind ofly can carry out light field scanning to carry out again the optical instrument of fluorescent scanning imperative.
Summary of the invention
For above-mentioned Problems existing, the object of this invention is to provide a kind of light field that can carry out and scan the optical texture that can carry out again fluorescent scanning, fluorescent optical system is attached in the optical system of digital pathological section scanner, achieves the function of digital pathological section scanner scanning fluorescence section.
The object of the invention is to be achieved through the following technical solutions:
First aspect of the present invention is to provide the optics that a kind of light field and fluorescence all can scan, and its structure comprises housing, camera, reflective mirror I, fluorescence camera, fluorescent moieties, reflective mirror II, guide rail, object lens.
The optics that light field and fluorescence all can scan, described reflective mirror II is between reflective mirror I and object lens.
The optics that light field and fluorescence all can scan, described guide rail and reflective mirror II link together, and move on the guideway for described reflective mirror II.
The optics that light field and fluorescence all can scan, described fluorescence camera is fluorescence microscopy camera.
The optics that light field and fluorescence all can scan, described fluorescence camera, fluorescent moieties, reflective mirror II are that straight line is placed, the bearing of trend of described guide rail and this straight line parallel.
The optics that light field and fluorescence all can scan, described reflective mirror I is positioned at the position light path of object lens being reflexed to described camera.
The principle of work of this optics is: during light field scanning, reflective mirror II is moved out of light path path by the slip of guide rail, and the light path of object lens directly reflexes to camera by reflective mirror I; During fluorescent scanning, reflective mirror II is moved to the position light path of object lens being reflexed to fluorescence camera by the slip of guide rail, the light path realizing object lens directly reflexes to fluorescence camera by reflective mirror II.
Second aspect of the present invention is to provide above-mentionedly can carry out light field scanning and can carry out again the pathological section scanner of the optics of fluorescent scanning a kind of comprising.
Compared with prior art, beneficial effect of the present invention:
A kind of light field that can carry out provided by the invention scans the optics that can carry out again fluorescent scanning, is attached to by fluorescent optical system in the optical system of digital pathological section scanner, achieves the function of digital pathological section scanner scanning fluorescence section.
Accompanying drawing explanation
Fig. 1 is the optical texture schematic diagram that only can carry out light field scanning in prior art;
Fig. 2 can carry out the structural representation that light field scanning can carry out again the optical texture of fluorescent scanning.
Embodiment
Referring to accompanying drawing and concrete operations embodiment, the invention will be further described.
With reference to Fig. 2, a kind of light field that can carry out scans the optical texture that can carry out again fluorescent scanning, its structure comprises housing 1, camera 2, reflective mirror I3, fluorescence camera 4, fluorescent moieties 5, reflective mirror II6, guide rail 7, object lens 8, light path 9, wherein, reflective mirror II6 is between reflective mirror I3 and object lens 8, and guide rail 7 and reflective mirror II6 link together, and described fluorescence camera 4, fluorescent moieties 5, reflective mirror II6 are that straight line is placed.
Can carry out the scan method that light field scanning can carry out again the optical texture of fluorescent scanning, during light field scanning, reflective mirror II6 be removed, light path is directly from object lens 8 to reflective mirror I3, and light path 9 is directly from object lens 8 to reflective mirror I3; During fluorescent scanning, reflective mirror II6 is moved on to the position in straight line with reflective mirror I3 and object lens 8.
Light path is transmitted into reflective mirror II6 from fluorescent moieties 5, object lens 8 are reflexed to by reflective mirror II6, be irradiated to fluorescence by object lens 8 again to cut into slices, fluorescent material in fluorescence section sends fluorescence and is irradiated to reflective mirror II6 by object lens 8 after exciting, reflex to fluorescent moieties 5 by reflective mirror II6 again, then light path 9 is injected fluorescence camera 4 and is carried out digitizing.
Be described in detail specific embodiments of the invention above, but it is as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (10)
1. light field and the fluorescence optics that all can scan, it is characterized in that, for the scanning of pathological section, these parts comprise housing, camera, reflective mirror I, fluorescence camera, fluorescent moieties, reflective mirror II, guide rail, object lens.
2. optics according to claim 1, is characterized in that, described reflective mirror II is between reflective mirror I and object lens.
3. optics according to claim 1, is characterized in that, described guide rail and reflective mirror II link together, and moves on the guideway for described reflective mirror II.
4. optics according to claim 1, is characterized in that, described fluorescence camera is fluorescence microscopy camera.
5. optics according to claim 1, is characterized in that, described fluorescence camera, fluorescent moieties, reflective mirror II are that straight line is placed, the bearing of trend of described guide rail and this straight line parallel.
6. optics according to claim 1, is characterized in that, described reflective mirror I is positioned at the position light path of object lens being reflexed to described camera.
7. a scan method for optical texture according to claim 1, is characterized in that, realizes light field scanning or fluorescent scanning by the position of mobile reflective mirror II.
8. the scan method of optical texture according to claim 7, is characterized in that, during described light field scanning, reflective mirror II is shifted out light path path by guide rail.
9. the scan method of optical texture according to claim 7, is characterized in that, during described fluorescent scanning, reflective mirror II is moved to the position light path of object lens being reflexed to fluorescence camera by guide rail.
10. one kind comprises and can to carry out the pathological section scanner that light field scanning can carry out again the optics of fluorescent scanning described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410693154.3A CN104375268A (en) | 2014-11-26 | 2014-11-26 | Optical structure capable of carrying out bright field scanning and fluorescence scanning |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410693154.3A CN104375268A (en) | 2014-11-26 | 2014-11-26 | Optical structure capable of carrying out bright field scanning and fluorescence scanning |
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| Publication Number | Publication Date |
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| CN104375268A true CN104375268A (en) | 2015-02-25 |
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| CN201410693154.3A Pending CN104375268A (en) | 2014-11-26 | 2014-11-26 | Optical structure capable of carrying out bright field scanning and fluorescence scanning |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105487218A (en) * | 2015-12-28 | 2016-04-13 | 宁波舜宇仪器有限公司 | Microscopic scanner and microscopic scanning method |
| CN105486683A (en) * | 2015-12-28 | 2016-04-13 | 宁波舜宇仪器有限公司 | Mycobacterium tuberculosis screening system and method |
| CN105629455A (en) * | 2015-12-28 | 2016-06-01 | 宁波舜宇仪器有限公司 | Microscopic scanner and microscopic scanning method |
| CN107493403A (en) * | 2017-08-11 | 2017-12-19 | 宁波江丰生物信息技术有限公司 | A kind of digital pathological section scanning system |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06347404A (en) * | 1993-06-08 | 1994-12-22 | Fuji Photo Film Co Ltd | Laser scanning fluorescence spectrophotometric measuring method and its device |
| KR20070105338A (en) * | 2005-01-19 | 2007-10-30 | 코닌클리케 필립스 일렉트로닉스 엔.브이. | Optical scanning device |
| CN101363839A (en) * | 2008-09-12 | 2009-02-11 | 首都师范大学 | Non-damage micrometering system for observating biological sample with fluorescent |
| JP2010091428A (en) * | 2008-10-08 | 2010-04-22 | Olympus Corp | Scanning optical system |
| CN102216827A (en) * | 2008-09-13 | 2011-10-12 | 独立行政法人科学技术振兴机构 | Microscope device and fluorescent observing method using same |
| CN102707085A (en) * | 2012-05-30 | 2012-10-03 | 中国科学技术大学 | Multifunctional particle image velocimeter based on laser confocal and bright-field microscope |
-
2014
- 2014-11-26 CN CN201410693154.3A patent/CN104375268A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06347404A (en) * | 1993-06-08 | 1994-12-22 | Fuji Photo Film Co Ltd | Laser scanning fluorescence spectrophotometric measuring method and its device |
| KR20070105338A (en) * | 2005-01-19 | 2007-10-30 | 코닌클리케 필립스 일렉트로닉스 엔.브이. | Optical scanning device |
| CN101363839A (en) * | 2008-09-12 | 2009-02-11 | 首都师范大学 | Non-damage micrometering system for observating biological sample with fluorescent |
| CN102216827A (en) * | 2008-09-13 | 2011-10-12 | 独立行政法人科学技术振兴机构 | Microscope device and fluorescent observing method using same |
| JP2010091428A (en) * | 2008-10-08 | 2010-04-22 | Olympus Corp | Scanning optical system |
| CN102707085A (en) * | 2012-05-30 | 2012-10-03 | 中国科学技术大学 | Multifunctional particle image velocimeter based on laser confocal and bright-field microscope |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105487218A (en) * | 2015-12-28 | 2016-04-13 | 宁波舜宇仪器有限公司 | Microscopic scanner and microscopic scanning method |
| CN105486683A (en) * | 2015-12-28 | 2016-04-13 | 宁波舜宇仪器有限公司 | Mycobacterium tuberculosis screening system and method |
| CN105629455A (en) * | 2015-12-28 | 2016-06-01 | 宁波舜宇仪器有限公司 | Microscopic scanner and microscopic scanning method |
| CN105629455B (en) * | 2015-12-28 | 2019-02-19 | 宁波舜宇仪器有限公司 | Micro- scanner and micro- scan method |
| CN107493403A (en) * | 2017-08-11 | 2017-12-19 | 宁波江丰生物信息技术有限公司 | A kind of digital pathological section scanning system |
| CN107493403B (en) * | 2017-08-11 | 2019-09-24 | 宁波江丰生物信息技术有限公司 | A kind of digital pathological section scanning system |
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Application publication date: 20150225 |
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