CN1043603A

CN1043603A - Granular mixture of thuringiensis bacillus

Info

Publication number: CN1043603A
Application number: CN 88105821
Authority: CN
Inventors: 朱邦雄
Original assignee: HUNAN INST OF MICROBE
Current assignee: HUNAN INST OF MICROBE
Priority date: 1988-12-24
Filing date: 1988-12-24
Publication date: 1990-07-11

Abstract

Granular mixture of thuringiensis bacillus is a kind of insecticide based on microorganism formulation.Granular mixture of thuringiensis bacillus be with the bacillus thuringiensis,Bt zymotic fluid after concentrating, be adsorbed on the sand of 20-40 purpose river, mix in 4: 1～19: 1 ratios and 3% carbofuran Granules then.Through a large number of experiments show that, this insecticide control corn borer effect is remarkable, every mu of particulate application agent 700g, and larval mortality reaches more than 95%, and mothproof bar efficient reaches more than 80%, and the drug effect phase reaches 15 days, and cost is 3.00 yuan.This insecticide control corn borer is generally used in the whole nation, and the dispenser expense can be saved 7,000 ten thousand yuan, and reduces the adverse consequences that chemical pesticide causes, is fit to the corn producing region and applies.

Description

Granular mixture of thuringiensis bacillus

Granular mixture of thuringiensis bacillus is a kind of insecticide based on microorganism formulation.

Corn borer is one of the primary pest in each corn producing region, the world, and China corn producing region reaches 20-30% because of the underproduction that corn borer harm causes.To the control of corn borer, units concerned are selecting to obtain certain progress aspect the kind of anti-corn borer, but are preventing and treating aspect the medicament of usefulness, the import furans pellet that do not have comparatively ideal insecticide to replace as yet to cost an arm and a leg, toxicity is big.

The existing report of bacillus thuringiensis,Bt control of maize snout moth's larva, but because the incubation period of corn borer is long, the position difference of the larva harm plant in the different length of times, therefore thuringiensis bacillus powder and the aqua that in the past used, its effective ingredient is difficult to reach in the lobus cardiacus clump of the hiding and harm of corn borer larvae, and the desinsection composition is easily by the sunlight passivation, the drug effect phase is short, has only 3-5 days, simultaneously the expenses for prevention and control costliness, be difficult to and the chemical pesticide competition, even so little large-area applications of also failing of toxicity.

The present invention is intended to develop a kind of bacillus thuringiensis,Bt intermixture, can prevent and treat the corn borer in the different length of times effectively according to unique life habit of corn borer, can reduce expense again, reduce toxicity.

Realize that the technical scheme that above-mentioned task adopts is: will be the bacillus thuringiensis,Bt microbial inoculum of carrier and 3% carbofuran Granules with 20-40 order river sand, and make a kind of granular mixture of thuringiensis bacillus with 4: 1～19: 1 ratios mixing; The preparation of bacillus thuringiensis,Bt microbial inoculum, be that its zymotic fluid successively secondary is concentrated through vacuum concentrator, thickening temperature≤60 ℃ for the first time, time≤50 minute, thickening temperature≤45 ℃ for the second time, the bacillus thuringiensis,Bt bacterium liquid after concentrating is in 1: 4 ratio, and usefulness 20-40 order river sand adsorbs, mix oven dry again, whole grain thoroughly.

The concrete production technology of granular mixture of thuringiensis bacillus is described in detail in detail below.

1, bacterial classification: the production bacterial classification is HD-1, separates from Unite States Standard powder HD-1-S-1980, is the bacterium pearl of Bacillus Thuringiensis.var.Kurstaki.

Bacillus thuringiensis strains is to the virulence difference of corn borer, and the HD-1 bacterial strain is very high to corn borer toxicity.

2, fermentation and cultivation:

The culture medium prescription of producing the bacillus thuringiensis,Bt microbial inoculum is as follows:

1) slant medium

Tryptones 2.5g yeast extract 2.5g

NH ₄CL 1.0g glucose 2.0g

MnCL ₂·H ₂O 6mg MgSO ₄·7H ₂O 200mg

CaCL ₂·2H ₂O 13mg Na ₂HPO ₄12.5mM

KH ₂PO ₄12.5mM H ₂O 1000ml

PH 6.8 agar 20g

2) fermentation tank culture medium

Corn steep liquor 1.0% starch 2.0%

Peptone 0.2% yeast extract 0.2%

Soybean cake powder 2.0% MgSO ₄7H ₂O 0.03%

FeSO ₄·7H ₂O 0.002% ZnSO ₄·7H ₂O 0.002%

CaCL ₃0.1% PH 7.0

In the medium that the fermentation tank fluid enlargement culture is used, descend, be beneficial to carrying out smoothly of the program of concentrating like this admittedly contain the content of thing.

The major parameter that the fermentation tank enlarged culture adopts is as follows:

29～31 ± 1 ℃ of cultivation temperature

Tank pressure 0.5kg/cm ²

Throughput 1: 0.4～1: 1.2

Putting jar time 80% gemma crystal comes off

3, concentrate

The zymotic fluid of putting behind the jar needs secondary concentration, and concentrate for the first time and can in the shell and tube vacuum concentrator, carry out, temperature≤60 ℃, the time of staying≤50 minute are concentrated in the scraper-type vacuum concentrator and carry out for the second time, temperature≤45 ℃.Zymotic fluid after concentrating, it contains the bacterium number is 120～16,000,000,000/ml.

4, absorption

Be adsorbed in the agitator and finish, the mixed solution of the Tween-80 of 0.1% polyvinyl alcohol and 0.01% is added in the concentrate, stir; In 1: 4 ratio,, mix thoroughly then with the sand absorption of 20-40 purpose river.Make the microbial inoculum carrier with 20-40 purpose river sand, can make the bacteria containing amount height, consumption is few, and effective bacillus thuringiensis,Bt granule is convenient to industrial production and long preservation, also is convenient to stabilized product quality.

5, oven dry

Dry in the common drying room of granular bacteria preparation at 40-60 ℃ of carrier absorption, its moisture should be less than 4～6%.

6, whole grain

Granule after the oven dry should be whole grain, be about to granule and cross 20 mesh sieves, the thick particle of failing to sieve discards the particle of failing to sieve at last through grinding after 20 mesh sieves, promptly makes bacillus thuringiensis,Bt granule to be mixed.

7, detect

The bacillus thuringiensis,Bt granule need descend the quality testing of routine project.

1) microscopy: oily mirror is observed down, and the gemma crystal is many, big, neat, the assorted bacterium of nothing;

2) plate count: every gram granule contains spore count alive and reaches 35-40 hundred million;

3) virulence bioassay: measure with silkworm egg paper tinsel method, 29 ℃ of probe temperatures, relative moisture 80-90%, 36 hours time, Lc50 is that every milliliter of content is no more than 10mg.

4) determination of moisture :≤4～6%

5) fineness: 98% particle≤20 orders

8, particle mixture moulding

With above-mentioned qualified after testing bacillus thuringiensis,Bt granule, in 4: 1～19: 1 ratios, with 3% commercially available carbofuran Granules mixing, and weighing and bagging on request.

Granular mixture of thuringiensis bacillus is applied in the lobus cardiacus of corn during use, the insecticide that contains the furans pellet then can be inhaled in the milpa, and the drug effect phase of particle mixture in the long controlled later stage is invaded the larva of lobus cardiacus clump; When milpa shook in wind, the medicament in the lobus cardiacus fell gradually because of the weight of particle itself, is retained in all the time in the lobus cardiacus clump, brings into play its drug effect.

Granular mixture of thuringiensis bacillus through in the Hunan, ground tests such as Zhejiang, Shandong, Beijing, control efficiency is remarkable.Every mu with particle mixture 700g, and larval mortality reaches more than 95%, the pulvis and the high 15-20% of aqua that produce than traditional handicraft; The drug effect phase reached more than 15 days, prolonged 10-12 days than the past product; Mothproof bar efficient reaches more than 80%; 3.00 yuan of cost per mus hang down 1.50～2.00 yuan than the expense of single use furans pellet.

Be below granular mixture of thuringiensis bacillus in Changsha, the part test result of Huayuan and zhejiang and other places.

Test shows, with granular mixture of thuringiensis bacillus control of maize snout moth's larva, can match in excellence or beauty with the furans pellet, is better than chemical pesticides such as acephatemet, dimehypo.China's maize sown area is hundred million mu of 2.7-2.9, has every year 1/4 area endangered by corn borer, as adopting granular mixture of thuringiensis bacillus control of maize snout moth's larva, can save 7,000 ten thousand yuan of dispenser expenses, and can reduce the harmful effect that chemical pesticide brings.

Claims

1, a kind of granular mixture of thuringiensis bacillus that is specifically designed to the control of maize snout moth's larva is characterized in that by being that the bacillus thuringiensis,Bt microbial inoculum of carrier and 3% carbofuran Granules mix with 20-40 order river sand, and its two mixed proportion is 4: 1～19: 1.

2, according to the described granular mixture of thuringiensis bacillus of claim 1; it is characterized in that the preparation of bacillus thuringiensis,Bt particle; be with its zymotic fluid in vacuum concentrator; respectively in temperature≤60 ℃; under the situation of time≤50 minute and temperature≤45 ℃ successively after the secondary concentration; adsorb, mix thoroughly with 20-40 order river sand in 1: 4 ratio, drying, whole grain form again.