CN104353509A - Multi-reagent colorimetric tube for portable colorimetric device - Google Patents

Multi-reagent colorimetric tube for portable colorimetric device Download PDF

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Publication number
CN104353509A
CN104353509A CN201410635755.9A CN201410635755A CN104353509A CN 104353509 A CN104353509 A CN 104353509A CN 201410635755 A CN201410635755 A CN 201410635755A CN 104353509 A CN104353509 A CN 104353509A
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China
Prior art keywords
reagent
steel ball
feed pipe
opening
colorimetric device
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CN201410635755.9A
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CN104353509B (en
Inventor
邹炳德
杨中柱
谢远敏
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NINGBO MEIKANG SHENGDE BIOTECHNOLOGY CO Ltd
Ningbo Medical System Biotechnology Co Ltd
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NINGBO MEIKANG SHENGDE BIOTECHNOLOGY CO Ltd
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Priority to CN201410635755.9A priority Critical patent/CN104353509B/en
Publication of CN104353509A publication Critical patent/CN104353509A/en
Priority to PCT/CN2015/080770 priority patent/WO2016074474A1/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention relates to a multi-reagent colorimetric tube for a portable colorimetric device. The multi-reagent colorimetric tube comprises an outer tube (1) used for storing a first reagent (1.1) and/or a to-be-detected sample, an inner tube (2) used for storing at least one of other reagents, and a steel ball (8) used for stirring and mixing the reagents, wherein an opening, into which the inner tube (2) is inserted, is formed in the upper end of the outer tube (1); the first reagent (1.1) is liquid, and other reagents are of solid structures; the steel ball (8) is positioned in the inner tube (2) which is internally provided with a first limiting structure which is used for storing other reagents and the steel ball in the inner tube (2); the steel ball (8) is positioned below other reagents; the upper end of the inner wall of the inner tube (2) is provided with an ejecting mechanism which is used for ejecting the steel ball (8) and other reagents out to enable the steel ball (8) and other reagents to drop into the first reagent (1.1).

Description

For many reagents ratios colour tube of portable colorimetric device
Technical field
The present invention relates to sample detection field, is specifically a kind of many reagents ratios colour tube for portable colorimetric device.
Background technology
At present, colorimetric cylinder is applied to sample chemical analysis and detects, by instrument, photometric detection is carried out to reacted colorimetric cylinder, thus determine the chemical composition of sample, colorimetric cylinder is widely used in environmental protection tests (as water quality of river detects), food quality detects, medical treatment detects or the detection field of other industry, portable colorimetric cylinder small volume, can be used for going out to carry, the sample at scene is detected, there is real-time, convenience.
Such as in many medical tests, sample to be measured is needed to mix with first liquid, be convenient to the interfering components in removal sample, start the first chemical reaction or biological respinse, when sample to be detected is with after first liquid mixing certain hour, add second liquid wherein, start the second chemical reaction or biological respinse, if when sample component to be detected is more complicated, even need interpolation the 3rd liquid or the 4th liquid, to start the 3rd reaction or the 4th reaction, whole process is by its chemical reaction of photometric measurement.Such as, glycosylated hemoglobin composition in test blood, need mix the molten sample crossing blood with R1 (first liquid), destroy the composition that may affect test in sample, then add R2 (second liquid) to react with it again, detect finally by photometric detection device.
For the photometric measurement of basic automation, the automatic analyzer of various complexity is had to measure, instrument will complete the application of sample of plurality of reagents, comprise complicated sample adding system, stirring system, purging system, heating system, cause whole instrument quite huge, complicated, price costly, and only has large hospital could operate by special door start-up; At present, existing one manually completes photometric measurement, the semi-automatic biochemical analyzer of application of sample, stirring, and its relative volume is less, and existing semi-automatic Biochemical Analyzer comprises mixer.
Colorimetric cylinder in biochemical analysis instrument apparatus for depositing and mixing sample to be detected and liquid, colorimetric cylinder comprises for depositing first liquid, steel ball and band detect the first mixer of sample, for depositing the second mixer of second liquid, when detecting, first mixer lid is taken off, second mixer is inserted the first mixer top, and the upper and lower of the second mixer is respectively equipped with the sealing-plug that can slide up and down at the second mixer inwall, when second liquid mixes with first liquid by needs, the sealing-plug on the second mixer top is enforced by the push rod in Biochemical Analyzer, thus drive the sealing-plug of second liquid and the second mixer bottom to move downward, when the sealing-plug of bottom departs from the second mixer, second liquid is because gravity is from the second mixer inside drippage, and mix with first liquid, again by be placed in advance steel ball in the first mixer coordinate in Biochemical Analyzer independent arrange the steel ball that moves in the first mixer of magnetic agitation dribbling rotate, thus mixed solution is stirred, mixed solution is reacted completely and stirs, finally carry out photometric detection, above prior art comes with some shortcomings part:
1) for needing strictly to control with the consumption and ratio of the aitiogenic first liquid of sample and second liquid, and above by promoting sealing-plug thus realizing the mode that first liquid mixes with second liquid, on the inwall making second liquid easily remain in the second mixer or sealing-plug, cause the consumption of the second liquid participating in reaction inaccurate, thus affect the reaction of first liquid and second liquid, finally cause the accuracy of photometric detection not high, on medical treatment detects, easily occur mistaken diagnosis or wrong report;
2) existing colorimetric cylinder is only only applicable to the mixing of two kinds of liquid reagents, can not carry out more than three kinds reagent tests;
3) because steel ball is encapsulated in the liquid in the first mixer in advance, and steel ball is being immersed in before in liquid, need to carry out specially treated to the surface of steel ball, and steel ball long period of soaking may make the composition of liquid change in liquid, and then the result of subsequent reactions may be affected.
Summary of the invention
Technical problem to be solved by this invention: provide a kind of photometric detection accuracy higher, be applicable to two or more reaction reagent and do not affect the many reagents ratios colour tube for portable colorimetric device of subsequent reactions.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions: a kind of many reagents ratios colour tube for portable colorimetric device, it comprises the outer tube for depositing the first reagent and detected sample and manages for depositing in other reagent of at least one, outer tube upper end is provided with the opening inserted for interior pipe, and other reagent are solid shape structure; It also comprises the steel ball for being uniformly mixed reagent, steel ball is positioned at pipe, the first position-limit mechanism for other reagent and steel ball being left in interior pipe is provided with in interior pipe, steel ball is positioned at below other reagent, and inner tube wall upper end is provided with and makes it fall into material jacking mechanism in the first reagent for steel ball and other reagent being ejected.
Adopt said structure, the present invention is advantageously: the wherein one of existing first reagent or detected sample is liquid, liquid sample or the mixed solution of the first reagent and sample only can be put in outer tube, the present invention changes the traditional concept that prior art adopts liquid reagent and reacted liquid reagent, adopt solid state reagents to fall into liquid reagent and mix post-reacted mode, the solid state reagents participating in reaction is made to mix with liquid reagent completely and fully react, prior art is there will not be to cause final photometric detection inaccurate because liquid reagent remains on inner tube wall, and then ensure that the accuracy of experiment, steel ball is left in interior pipe, because other reagent in interior pipe are solid structure, make steel ball can not affect the chemical composition of other reagent, thus follow-up chemical reaction can not be affected, and be positioned at below other reagent due to steel ball, when needs stir, by material jacking mechanism, steel ball is first headed in the first reagent of outer tube and the mixed solution of sample, to be coordinated with the magnetic agitation dish in Biochemical Analyzer by prior art steel ball and be uniformly mixed solution, material jacking mechanism is positioned at Biochemical Analyzer.
As preferably, described position-limit mechanism refers to slide axially the feed pipe be matched with in interior pipe, and described feed pipe lower end sidewall is axially arranged with opening along feed pipe, is provided with the cavity for depositing other reagent in opening, described first position-limit mechanism is positioned at opening, and material jacking mechanism is positioned at above feed pipe.
As preferably, described first position-limit mechanism comprises open bottom and is provided with at least two for placing the radial groove of steel ball and other reagent along feed pipe length direction.
As preferably, other reagent described are chondritic, and described radial groove is arc groove, and the diameter of described steel ball and other reagent is greater than the distance between open bottom to inner tube wall.
The present invention adopts feed pipe to be slidably fitted in inner tube wall, and feed pipe lower end sidewall is provided with opening, is provided with number and solid state reagents and steel ball radial groove one to one in opening, and radial groove is used for placing and spacing solid state reagents and steel ball, described solid state reagents is chondritic, radial groove is arc groove, other reagent of steel ball and chondritic are positioned at arc groove, and be greater than the distance between open bottom to inner tube wall due to other reagent of chondritic and the diameter of steel ball, other reagent of chondritic and steel ball is made to be limited in the opening of feed pipe by the clamping of inner tube wall and arc groove, when other reagent and steel ball add in the first reagent by needs, feed pipe is promoted downwards by material jacking mechanism, when the opening of feed pipe side wall lower ends departs from inner tube wall, other reagent of chondritic and steel ball freely fall into the first reagent owing to losing the clamping of inner tube wall in arc groove, finally mixer is put into biochemical analysis instrument apparatus, by the magnetic agitation dish in steel ball and Biochemical Analyzer, mixed solution is stirred, it is made fully to react, finally carry out photometric detection, structure is simpler.
As preferably, the number of described arc groove is four, and the number of other reagent is three, and steel ball is positioned at the arc groove of bottommost.
As preferably, described three other reagent are the second reagent.
As preferably, described three other reagent are respectively the second reagent and the 3rd reagent, and described second reagent is between steel ball and the 3rd reagent.
Existing colorimetric cylinder is only only applicable to the mixing of two kinds of liquid, and the present invention adopts the mode of solid reagent and liquid reagent, make in feed pipe, to deposit other reagent that two or more participates in reaction, progressively feed pipe is promoted by material jacking mechanism, make it fall in the first reagent successively by needing other reagent of reaction and participate in reaction, structure of the present invention is applicable to the reagent that three kinds or more and detected sample react, the many reagent realizing the small-sized spectrophotometric sensing device of prior art detect, the scope of application is wider, without the need to changing interior pipe successively, and it is simple to operate.
As preferably, described feed pipe upper end outer wall is provided with at least one annular seal groove, is arranged with sealing ring in annular seal groove, and sealing is better, and makes to have certain frictional force between feed pipe and interior pipe, makes feed pipe can not free landing in interior pipe.
As preferably, described interior pipe lower ending opening is sliding combined with sealing-plug.
Accompanying drawing explanation
Fig. 1 is the detonation configuration schematic diagram of the present invention for many reagents ratios colour tube of portable colorimetric device.
Fig. 2 is the structural representation of the present invention for many reagents ratios colour tube of portable colorimetric device.
Status architecture schematic diagram when Fig. 3 is to be added second reagent of the present invention for many reagents ratios colour tube of portable colorimetric device.
State machine schematic diagram when Fig. 4 is to be added three reagent of the present invention for many reagents ratios colour tube of portable colorimetric device.
Fig. 5 is the side elevational cross-section structural representation of the present invention for many reagents ratios colour tube of portable colorimetric device.As shown in the figure: 1-outer tube, 1.1-first reagent, pipe in 2-, 2.1-second reagent, 2.2-the 3rd reagent, 3-sealing-plug, 4-feed pipe, 4.1-opening, 5-arc groove, 6-annular seal groove, 7-sealing ring, 8-steel ball.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described further.
In the present description, it will be appreciated that, term " on ", D score, " interior ", the indicating position such as " outward " or position relationship be based on the position relationship described in accompanying drawing, be only the present invention for convenience of description or simplified characterization, instead of indicate the specific orientation that must have.
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, a kind of many reagents ratios colour tube for portable colorimetric device, it comprises outer tube 1 for depositing the first reagent 1.1 and detected sample and for depositing pipe 2 in other reagent of at least one, outer tube 1 upper end is provided with the opening inserted for interior pipe 2, and other reagent are solid shape structure; It also comprises the steel ball 8 for being uniformly mixed reagent, steel ball 8 is positioned at pipe 2, the first position-limit mechanism for other reagent and steel ball 8 being left in interior pipe 2 is provided with in interior pipe 2, steel ball 8 is positioned at below other reagent, and interior pipe 2 inwall upper end is provided with and makes it fall into material jacking mechanism in the first reagent 1.1 for being ejected by other reagent of steel ball 8.
The present invention changes the traditional concept that prior art adopts liquid reagent and reacted liquid reagent, adopt solid state reagents to fall into liquid reagent and mix post-reacted mode, the solid state reagents participating in reaction is made to mix with liquid reagent completely and fully react, there will not be prior art to cause final photometric detection inaccurate because liquid reagent remains on inner tube wall, and then ensure that the accuracy of experiment; Steel ball 8 is left in interior pipe 2, because other reagent in interior pipe 2 are solid structure, make steel ball 8 can not affect the chemical composition of other reagent, thus follow-up chemical reaction can not be affected, and be positioned at below other reagent due to steel ball 8, when needs stir, by material jacking mechanism, steel ball 8 is first headed in the first reagent 1.1 of outer tube 1 and the mixed solution of sample, to be coordinated with magnetic agitation dish by prior art steel ball 8 and be uniformly mixed solution.
Described position-limit mechanism refers to slide axially the feed pipe 4 be matched with in interior pipe 2, described feed pipe 4 lower end sidewall is axially arranged with opening 4.1 along feed pipe 4, the cavity for depositing other reagent is provided with in opening 4.1, described first position-limit mechanism is positioned at opening 4.1, and material jacking mechanism is positioned at above feed pipe 4; Described first position-limit mechanism comprises bottom opening 4.1 and is provided with at least one for placing the radial groove of other reagent along feed pipe 4 length direction; Other reagent described are chondritic, and described radial groove is arc groove 5, and the diameter of other reagent described is greater than bottom opening 4.1 to the distance between interior pipe 2 inwall.
The present invention adopts feed pipe 4 to be slidably fitted in interior pipe 2 inwall, and feed pipe 4 lower end sidewall is provided with opening 4.1, is provided with number and solid state reagents and steel ball 8 radial groove one to one in opening 4.1, and radial groove is used for placing and spacing solid state reagents and steel ball 8, described solid state reagents is chondritic, radial groove is arc groove 5, other reagent of chondritic and steel ball are positioned at arc groove 5, and be greater than bottom opening 4.1 to the distance between interior pipe 2 inwall due to other reagent of chondritic and the diameter of steel ball 8, other reagent of chondritic and steel ball 8 is made to be limited in the opening 4.1 of feed pipe 4 by the clamping of interior pipe 2 inwall and arc groove 5, when steel ball 8 and other reagent add in the first reagent 1.1 by needs, feed pipe 4 is promoted downwards by material jacking mechanism, when the opening 4.1 of feed pipe 4 side wall lower ends departs from interior pipe 2 inwall, other reagent of chondritic and steel ball freely fall into the first reagent 1.1 owing to losing the clamping of interior pipe 2 inwall in arc groove 5, finally mixer is put into biochemical analysis instrument apparatus, by the magnetic agitation dish in steel ball 8 and Biochemical Analyzer, mixed solution is stirred, it is made fully to react, finally carry out photometric detection, structure is simpler.
The present invention adopts feed pipe 4 to carry out spacing to other spherical reagent, also can carry out spacing to other solid-state reagent by other means, as interior pipe 2 inwall is provided with spacing ring along axis, other spherical reagent are positioned at above spacing ring, spacing ring is flexible material, by material jacking mechanism, action is ejected to other reagent, the cooperation really up to the mark above spacing ring of other reagent is headed in the first reagent 1.1.
Described arc groove 5 for number be four, the number of other reagent is three, and steel ball 8 is positioned at the arc groove 5 of bottommost.
Described three other reagent are the second reagent 2.1.
Described three other reagent are respectively the second reagent 2.1 and the 3rd reagent 3.1, and described second reagent 2.1 is between steel ball and the 3rd reagent 2.2.
The number of arc groove can increase along with the length of reagent, thus the quantity of other reagent can be increased, existing colorimetric cylinder is only only applicable to the mixing of two kinds of liquid, and the present invention adopts the mode of solid reagent and liquid reagent, make in feed pipe 4, to deposit other reagent that two or more participates in reaction, feed pipe 4 is progressively promoted by material jacking mechanism, make it fall in the first reagent .1.1 successively by needing other reagent of reaction and participate in reaction, structure of the present invention is applicable to the reagent that three kinds or more and detected sample react, the scope of application is wider, without the need to changing interior pipe 2 successively, and it is simple to operate, meanwhile, other reagent can be also same reagent, and making colorimetric cylinder of the present invention also can complete prior art needs two kinds and detected sample response situation, the present invention only enumerates the second reagent and the 3rd reagent in addition, also can have other reagent such as the 4th reagent and the 5th reagent.
The concrete operation step that the present invention adds reagent is as follows:
1. take off outer tube 1 capping, interior pipe 2 is inserted outer tube 1 top;
2. the colorimetric cylinder assembled is put into biochemical analysis, promote feed pipe 4 certain distance by the material jacking mechanism in Biochemical Analyzer, make the steel ball 8 being positioned at feed pipe 4 foot fall into the mixed solution of the first reagent 1.1 and sample;
3. the magnetic vibration dribbling in Biochemical Analyzer is moved steel ball 8 and is rotated in outer tube 1, makes it fully react for stirring the first reagent 1.1 with sample;
4. after the first reagent 1.1 and example reaction are complete, continue to promote feed pipe 4, other reagent in feed pipe 4 are progressively joined the first reagent 1.1 with in the mixed solution of sample;
5. repeat above-mentioned steps 3. and 4. to operate, make other reagent progressively joined in mixed solution participate in chemical reaction successively;
6. photometric detection is carried out.
Described feed pipe 4 upper end outer wall is provided with at least one annular seal groove 6, is arranged with sealing ring 7 in annular seal groove 6, and sealing is better, and makes to have certain frictional force between feed pipe 4 and interior pipe 2, makes feed pipe 4 can not free landing in interior pipe 2.
Described interior pipe 2 lower ending opening is sliding combined with sealing-plug 3.
The solidification processing mode of other reagent that the present invention adopts can adopt liquid nitrogen frozen or other existingly can make the solidified mode of liquid reagent.
In addition, the portable many reagents ratios colour tube of the present invention can also be applied to other detection industry, and as food quality detection or the detection of environmental protection water quality of river etc., operating procedure and aforesaid operations step duplicate; If when only putting into liquid detection sample (as detected water quality of river) in outer tube, river sample can be only placed with by outer tube, and reagent is all put in interior pipe, react one by one finally by aforesaid operations step and water quality is detected, detect diversity, the scope of application is large, more convenient.
Be described the present invention and embodiment thereof above, this description does not have restricted, and shown in accompanying drawing is also one of embodiments of the present invention, and actual structure is not limited thereto.If generally speaking those of ordinary skill in the art enlightens by it, when not departing from the invention aim, designing the frame mode similar to this technical scheme and embodiment without creationary, all should protection scope of the present invention be belonged to.

Claims (9)

1. the many reagents ratios colour tube for portable colorimetric device, it is characterized in that: it comprises the outer tube (1) for depositing the first reagent (1.1) and/or detected sample and manages (2) for depositing in other reagent of at least one, outer tube (1) upper end is provided with the opening inserted for interior pipe (2), first reagent (1.1) is liquid, and other reagent are solid shape structure; It also comprises the steel ball (8) for being uniformly mixed reagent, steel ball (8) is positioned at pipe (2), the first position-limit mechanism for other reagent and steel ball being left in interior pipe (2) is provided with in interior pipe (2), steel ball (8) is positioned at below other reagent, and interior pipe (2) inwall upper end is provided with and makes it fall into material jacking mechanism in the first reagent (1.1) for steel ball (8) and other reagent being ejected.
2. the many reagents ratios colour tube for portable colorimetric device according to claim 1, it is characterized in that: described position-limit mechanism refers to slide axially the feed pipe (4) be matched with in interior pipe (2), described feed pipe (4) lower end sidewall is axially arranged with opening (4.1) along feed pipe (4), the cavity for depositing other reagent is provided with in opening (4.1), described first position-limit mechanism is positioned at opening (4.1), and material jacking mechanism is positioned at feed pipe (4) top.
3. the many reagents ratios colour tube for portable colorimetric device according to claim 2, is characterized in that: described first position-limit mechanism comprises opening (4.1) bottom and is provided with at least two for placing the radial groove of steel ball (8) and other reagent along feed pipe (4) length direction.
4. the many reagents ratios colour tube for portable colorimetric device according to claim 3, it is characterized in that: other reagent described are chondritic, described radial groove is arc groove (5), and the diameter of described steel ball (8) and other reagent is greater than the distance between opening (4.1) bottom to interior pipe (2) inwall.
5. the many reagents ratios colour tube for portable colorimetric device according to claim 4, it is characterized in that: described arc groove (5) for number be four, the number of other reagent is three, and steel ball (8) is positioned at the arc groove (5) of bottommost.
6. the many reagents ratios colour tube for portable colorimetric device according to claim 5, is characterized in that: described three other reagent are the second reagent (2.1).
7. the many reagents ratios colour tube for portable colorimetric device according to claim 5, it is characterized in that: described three other reagent are respectively the second reagent (2.1) and the 3rd reagent (2.2), and described second reagent (2.1) is positioned between steel ball and the 3rd reagent (2.2).
8. the many reagents ratios colour tube for portable colorimetric device according to claim 1, it is characterized in that: described feed pipe (4) upper end outer wall is provided with at least one annular seal groove (6), is arranged with sealing ring (7) in annular seal groove (6).
9. the many reagents ratios colour tube for portable colorimetric device according to claim 1, is characterized in that: described interior pipe (2) lower ending opening is sliding combined with sealing-plug (3).
CN201410635755.9A 2014-11-12 2014-11-12 Many reagent color comparison tube for portable colorimetric device Active CN104353509B (en)

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CN201410635755.9A CN104353509B (en) 2014-11-12 2014-11-12 Many reagent color comparison tube for portable colorimetric device
PCT/CN2015/080770 WO2016074474A1 (en) 2014-11-12 2015-06-04 Multi-reagent colorimetric tube for portable colorimetric device

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Application Number Priority Date Filing Date Title
CN201410635755.9A CN104353509B (en) 2014-11-12 2014-11-12 Many reagent color comparison tube for portable colorimetric device

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CN104353509B CN104353509B (en) 2016-06-22

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WO2016074474A1 (en) * 2014-11-12 2016-05-19 宁波美康盛德生物科技有限公司 Multi-reagent colorimetric tube for portable colorimetric device
CN108792258A (en) * 2018-05-21 2018-11-13 南京申友生物技术有限公司 A kind of genetic test kit

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WO2016074474A1 (en) * 2014-11-12 2016-05-19 宁波美康盛德生物科技有限公司 Multi-reagent colorimetric tube for portable colorimetric device
CN108792258A (en) * 2018-05-21 2018-11-13 南京申友生物技术有限公司 A kind of genetic test kit

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