CN104351247B - A kind of short-tube lycoris bulb tillering accelerator and using method thereof - Google Patents
A kind of short-tube lycoris bulb tillering accelerator and using method thereof Download PDFInfo
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Abstract
The invention discloses a kind of short-tube lycoris bulb tillering accelerator and application process thereof, described adjusting control agent is 6-benzyl amino-9-(oxinane-2-yl)-9H-purine, tributyl (the chloro-2-thiophene of 5-) phosphorus chloride and auxiliary agent sucrose, wherein 6-benzyl amino-9-(oxinane-2-yl)-9H-purine: the mixture (referring to the mass ratio of 100% purity) that the mass ratio of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is 2.0～2.5:1.0～1.5, preferred proportion is 2.2:1.3. When plantation, after adjusting control agent is watered to 10L and fully dissolves and mix by every 2.5～3.5g, soak bulb and root system 5～8min, can promote to tiller, accelerate to take root, can significantly improve the bulb amount of tillering; When immersion, can not be placed on the place of direct light, should be placed on the place without direct light under tree shade or in cool canopy and soak; This adjusting control agent is produced upper easy to use, simple to operate, and cost is low, and the effect that short short-tube lycoris bulb is tillered is remarkable.
Description
Technical field
The invention belongs to agriculture practical new technology research and development and application.
Background technology
Short-tube lycoris (LycorisradiataHerb) has another name called crow garlic, Lycoris radiata etc., is the perennial squama of Amaryllidaceae LycorisStem herbaceous plant. It is to extract valuable bio-pharmaceuticals raw material lycorine (lycorine), galanthamine (galanthamine), stoneThe medicinal plant of garlic amine alkali (lycora-mine) etc. is also a kind of large and gorgeous good landscape flower plant of spending. Lycorine, addThat Lan Tamin, Dihydrogalantnamine have is anticancer, anti-inflammatory, anti-amoebic dysentery drug effect, the only current international market of high-purity galanthamine valency90～100 dollars/gram. Domestic factory extracts alkaloid and is all by excavating wild short-tube lycoris, because of a large amount of all the year round as afforestationExcavate, wild resource is on the verge of exhaustion, and place of production massif is dug riddled with gaping woundsly, and environmental disruption is serious, and plant produced is also difficult to continueDevelopment, for this reason, some factory sets up short-tube lycoris raw material artificial cultivation production base, but that bulb is tillered is very slow, and benefit is low.
At present, the domestic report of the research to short-tube lycoris is very few, and is confined to form, physiology more and introduces a fine variety breeding above, and cultivation is arrangedExecute is only single rich water quality management. Therefore, also do not form so far at home the short-tube lycoris production base of scale. Mostly be it abroadAlkaloidal pharmacological research, produce fresh (cutting) flower etc. External in the extraction of maryllidaceous alkaloid and starch and the research of application aspectMore, and comparatively deep.
China's short-tube lycoris germ plasm resource is abundant, and Amaryllidaceae Lycoris (LycorisHerb) plant whole world distribution more than 20 is planted,Main product China, minority is in Japan, Burma, Korea and China Taiwan, and China has 15 kinds, has comparative advantage, and concentrates and is distributed in the Changjiang riverIn The Middle And Lower Reaches, our province Jiuyi Shan Mountain area short-tube lycoris germ plasm resource, through preliminary examination, have 4 more than kind, and quantity is larger. ButScientific research, production utilize and industrialization aspect has lagged behind other countries and area, and we are necessary to strengthen grinding of this respectStudying carefully and industrialization, to accelerate the exploitation of China's natural products, is the development service of national economy.
Its bulb of the artificial cultivation of short-tube lycoris is tillered very slow, and output is extremely low, there is no good solution route. Solve thisOne problem, sprays short-tube lycoris bulb tillering accelerator and is it compared with one of effective measures, and easy to use, simple to operate, and cost is low,The short effect of tillering is remarkable. But about this research on the one hand have not been reported.
Summary of the invention
Object of the present invention aims to provide the promoter that a kind of short-tube lycoris bulb is tillered, easy to use, simple to operate, costLow, the short bulb effect of tillering is remarkable. Another object of the present invention aims to provide above-mentioned short-tube lycoris bulb tillering accelerator and preferably shouldUse method.
Its bulb of the artificial cultivation of short-tube lycoris is tillered very slow, and output is extremely low, there is no good solution route. Solve thisOne problem, uses short-tube lycoris bulb tillering accelerator and is it compared with one of effective measures, and easy to use, simple to operate, and cost is low,The short bulb effect of tillering is remarkable.
The object of the invention is to realize in the following manner:
Adjusting control agent of the present invention is 6-benzyl amino-9-(oxinane-2-yl)-9H-purine [6-benzylamino-9-(tetrahydropyran-2-yl)-9H-purine], tributyl (the chloro-2-thiophene of 5-) phosphorus chloride [tributyl (5-Chloro-2-thienyl) phosphoniumchloride] and auxiliary agent sucrose composition, wherein 6-benzyl amino-9-(tetrahydrochysene pyrroleMutter-2-yl)-9H-purine: mass ratio 2.0～2.5:1.0～1.5 of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride for preferably thanExample.
Sucrose in adjusting control agent, mainly as auxiliary agent, plays preparation filling and stable, can enter according to production applicationRow adds. Inventor studies and finds to add auxiliary agent sucrose and 6-benzyl amino-9-(oxinane-2-yl)-9H-purine: tributylMass ratio 0.5～3.0:2.0～2.5:1.0～1.5 of (the chloro-2-thiophene of 5-) phosphorus chloride are preferred ratio. Above three'sMost preferred mass ratio is 2.0:2.2:1.3. Adjusting control agent is watered 10L by every 2.5～3.5g, wherein auxiliary agent sucrose and 6-benzyl ammoniaBase-9-(oxinane-2-yl)-9H-purine: the mass ratio of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is 0.5～3.0:2.0～2.5:1.0～1.5。
The present invention selects plant bulb tillering accelerator 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and three fourthsBase (the chloro-2-thiophene of 5-) phosphorus chloride and auxiliary agent glucose sugar, adopt particularly by 6-benzyl amino-9-(oxinane-2-yl)-9H-Purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chloride has adopted the compatibility of suitable concentration, is mixed with commercial adjusting control agent, to short-tube lycorisBulb is tillered and is promoted, activates the division of its bulb bud primordial cell, improves its division speed, suppresses metabolic exhaustion, improvesThe bulb speed of tillering, has solved the problem that short-tube lycoris artificial cultivation bulb is tillered slowly, yielded poorly, and this will carry for short-tube lycoris artificial cultivationHigh yield lays the first stone. This adjusting control agent is produced upper easy to use, simple to operate, and cost is low, and the short bulb effect of tillering is remarkable.
Short-tube lycoris bulb tillering accelerator formula is 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (5-Chloro-2-thiophene) phosphorus chloride and sucrose mixes by the ratio of above-mentioned mass ratio, plastic bag sealing packaging, shady and cool dry lucifuge place is protectedDeposit 1 year shelf-life. 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chloride andThe pure percentage composition of sucrose does not have particular/special requirement, and its impurity is not affecting under the prerequisite of its stability, reaches formula institute when compatibilityThe net content requiring. It is below the comparative test of its regulating effect.
Short-tube lycoris bulb tillering accelerator of the present invention has no the report of like product at present. The present invention is that inventor grinds for many yearsThe result of the test that the work of studying carefully draws, and be verified in the production of long period, effect is better. And formula of the present inventionThe content that also possesses skills is higher, and formulation is more stable, and raw material sources are abundant, easy and simple to handle, as are developed to commodity, low price. ?Easy to use in production, regulating effect is remarkable, and use cost is low, is a kind of good short-tube lycoris bulb tillering accelerator. StoneGarlic bulb tillering accelerator formula can carry out batch production production, and it develops not only will increase a kind of novel bulb on marketThe type of tillering promoter, also will provide the approach of a solution for the problem that it is difficult that short-tube lycoris artificial cultivation is tillered.
Application process of the present invention is: when plantation, the adjusting control agent having mixed is watered to 10L by every 2.5～3.5g(10kg) fully dissolve and mix, after being watered, will now cash use, soak bulb and root system 5～8min, auxiliary agent sucrose and 6-benzylAmino-9-(oxinane-2-yl)-9H-purine: the mass ratio of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is 0.5～3.0:2.0～2.5:1.0～1.5. When immersion, can not be placed on the place of direct light, should be placed under tree shade or in cool canopy without direct lightSoak in place. Below to invent artificially to realize object of the present invention, constantly select by experiment and select be applicable to thisThe raw material of the corresponding adjusting control agent formula that the short bulb of alum garlic artificial cultivation is tillered and the regulating effect that relevant proportioning is done thereofComparative test.
1. single dose test
1.1 materials and methods
1.1.1 test material
Test short-tube lycoris (LycorisradiataHerb) maternal plant is to reserve seed for planting in this base, and test material is its natural propagationThe plant and the bulb that come. Test with the plant bulb conditioning agent of tillering is: 6-benzyl amino-9-(oxinane-2-yl)-9H-purine;Tributyl (the chloro-2-thiophene of 5-) phosphorus chloride kinetin; Kinetin; Methyl α-naphthyl acetate (NAA).
1.1.2 test method
Test is carried out in Institutes Of Technology Of Changsha's Jiuyi Shan Mountain proving ground. Excavate short-tube lycoris bulb kind from Jiuyi Shan Mountain proving groundBall, is watered 10L adjusting control agent by following concentration and fully dissolves and mix, and puts into plastic tub or Plastic Drum, after being watered, will now cashWith; Soak bulb and root system 5～8min; When immersion, can not be placed on the place of direct light, should be placed on nothing under tree shade or in cool canopySoak in the place of direct light. After having soaked, take out planting ball, the density that is respectively 10cm, 20cm with seeding row spacing is planted againIn the smooth soil block of its proving ground medium fertility level, a kind ball of every root and stem of certain plants kind, conventional rich water quality management and the prevention and control of plant diseases, pest control.12nd month time, gather in the crops, the bulbec number that its bulb mother bulb is tillered is counted and (diameter is reached to dividing of the above size of 1cmTiller bulbec number is counted, and diameter is less than 1cm person and does not calculate therein); To occur on bulbec chassis fibrous root numberCount that (fibrous root occurring with bulbec chassis after mother bulb is taken off is as the criterion, and is less than the long fibrous root of 0.5cm and disregardsCalculate therein).
Each medicine setting is 4 kinds of concentration gradients from low to high---in every 10L water, add respectively 100% sterling mass ratio6-benzyl amino-9-(oxinane-2-yl)-9H-purine: 1.0g, 1.5g, 2.0g, 2.5g; Tributyl (the chloro-2-thiophene of 5-) chlorineChange phosphorus: 0.5g, 1.0g, 1.5g, 2.0g; Kinetin: 0.5g, 1.0g, 1.5g, 2.0g; NAA:0.5g, 1.0g, 1.5g, 2.0g.
Test arranges 17 processing (comprising contrast), and 3 of each processing repeat (6m2/ repeat). Each reagent is watered now to be converted afterwardsNow use, soaks bulb and root system 5～8min. Each reagent concentration of processing and processing facilities are following, and (following processing is gotGrams all refer to that 100% sterling quality is dissolved in the amount in 10L water, i.e. concentration): process 1:1.0g6-benzyl amino-9-(tetrahydrochysene pyrroleMutter-2-yl)-9H-purine; Process 2:2.0g6-benzyl amino-9-(oxinane-2-yl)-9H-purine; Process 3:2.2g6-benzylAmino-9-(oxinane-2-yl)-9H-purine; Process 4:2.5g6-benzyl amino-9-(oxinane-2-yl)-9H-purine;Process 5:0.5g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride; Process 6:1.3g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride; Process7:1.5g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride; Process 8:2.0g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride; Process 9:0.5g kinetin; Process 10:1.0g kinetin; Process 11:2.2g kinetin; Process 12:2.5g kinetin; Process 13:0.5gNAA; Process 14:1.0gNAA; Process 15:2.2gNAA; Process 16:2.5gNAA; Contrast (CK): soak with running water.
1.2 statistical analysis
Test data is adopted to SAS(6.12) carry out statistical analysis.
Process the chemical regulation effect of 12 months (i.e. 1 growth cycle) rear short-tube lycoris in table 1. Result of the test shows, is locatingManaging after 12 months (i.e. 1 growth cycle), there is significant difference to the regulating effect of short-tube lycoris in different plant bulb tillering accelerators,The bulb tiller number of processing 3 and processing 6 and bulbec fibrous root number are all higher than other processing. The regulating effect of these 2 processing is describedGood, i.e. the debita spissitudo of 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chlorideRegulating effect better.
Table 1. is processed the chemical regulation effect of 12 months (i.e. 1 growth cycle) rear short-tube lycoris
Note: lowercase is 0.05 level, the different letter representation significant differences of same row, lower same.
2. compatibility test
2.1 materials and methods
2.1.1 test material
Test short-tube lycoris (LycorisradiataHerb) maternal plant is to reserve seed for planting in this base, and test material is its natural propagationThe plant and the bulb that come. Test with the plant bulb conditioning agent of tillering is: 6-benzyl amino-9-(oxinane-2-yl)-9H-purineWith tributyl (the chloro-2-thiophene of 5-) phosphorus chloride.
2.1.2 test method
Test is carried out in Institutes Of Technology Of Changsha's Jiuyi Shan Mountain proving ground. Excavate short-tube lycoris bulb kind from Jiuyi Shan Mountain proving groundBall, by following 1～9 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of the 5-) chlorine processedChange after the grams mixing of phosphorus, plastic bag sealing packaging, shady and cool lucifuge place preserved after 1 year, unpacked, and each processing is dissolved in respectivelyIn 10L water, short-tube lycoris kind ball is processed. The adjusting control agent having mixed is watered to 10L by following concentration and fully dissolves and mix, putEnter in plastic tub or Plastic Drum, after being watered, will now cash use; Soak bulb and root system 5～8min; When immersion, can not be placed on straightPenetrate the place of light, should be placed on the place without direct light under tree shade or in cool canopy and soak. After having soaked, take out planting ball, useThe density that seeding row spacing is respectively 10cm, 20cm is planted in the smooth soil block of its proving ground medium fertility level again, every root and stem of certain plants kind 1Individual kind of ball, conventional rich water quality management and the prevention and control of plant diseases, pest control. 12nd month time, gather in the crops the bulbec number that its bulb mother bulb is tilleredCount (the bulbec number of tillering that diameter is reached to the above size of 1cm is counted, and diameter is less than 1cm person and does not calculate therein);To occur on bulbec chassis fibrous root number count (occur with bulbec chassis after mother bulb is taken off oneInferior fibrous root is as the criterion, and is less than long not the calculating therein of 0.5cm).
With 3 of 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chlorideConcentration gradient is carried out compatibility test, to determine whether they have cooperative effect. Each medicine setting is 4 kinds of concentration from low to highGradient---in every 10L water, add 100% sterling quality 6-benzyl amino-9-(oxinane-2-yl)-9H-purine; Tributyl (5-Chloro-2-thiophene) phosphorus chloride. Test arranges 10 processing (comprising contrast), and 3 of each processing repeat (6m2/ repeat). Each reagentBe watered the rear use of now cashing, soak bulb and root system 5～8min. Each spraying concentration of processing and compatibility situation are following (below everywhereThe grams that reason is got all refers to that 100% sterling quality is dissolved in the amount in 10L water, i.e. concentration):
Process 1:2.0g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.0g tributyl (the chloro-2-thiophene of 5-)Phosphorus chloride; Process 2:2.2g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.0g tributyl (the chloro-2-thiophene of 5-) chlorineChange phosphorus; Process 3:2.5g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.0g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Process 4:2.0g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.3g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Process 5:2.2g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.3g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Process 6:2.5g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.3g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Process 7:2.0g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.5g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Process 8:2.2g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.5g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Process 9:2.5g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.5g tributyl (the chloro-2-thiophene of 5-) chlorinationPhosphorus; Contrast (CK): soak with running water.
2.2 statistical analysis
Test data is adopted to SAS(6.12) carry out statistical analysis.
Process the chemical regulation effect of 12 months (i.e. 1 growth cycle) rear short-tube lycoris in table 2. Result of the test shows, is locatingManaging after 12 months (i.e. 1 growth cycle), there is significant difference to the regulating effect of short-tube lycoris in different compatibilities, and the bulb of processing 5 dividesTiller number and bulbec fibrous root generation number are all higher than other processing. Regulation and control synergy the best of processing 5 is described, i.e. 2.2g6-benzyl ammoniaThe regulation and control cooperative effect of base-9-(oxinane-2-yl)-9H-purine+1.3g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride not onlyFor positive-effect, and its regulating effect will be got well compared with other processing.
Table 2. is processed the chemical regulation effect of rear short-tube lycoris 12 months (1 bulb is tillered the cycle)
3. auxiliary agent test
3.1 materials and methods
3.1.1 test material
Test short-tube lycoris (LycorisradiataHerb) maternal plant is to reserve seed for planting in this base, and test material is its natural propagationThe plant and the bulb that come. Test with the plant bulb conditioning agent of tillering is: 6-benzyl amino-9-(oxinane-2-yl)-9H-purineWith tributyl (the chloro-2-thiophene of 5-) phosphorus chloride.
3.1.2 test method
Test is carried out in Institutes Of Technology Of Changsha's Jiuyi Shan Mountain proving ground. Excavate short-tube lycoris bulb kind from Jiuyi Shan Mountain proving groundBall, 6-benzyl amino-9-(oxinane-2-yl)-9H-purine, tributyl (the chloro-2-thiophene of 5-) phosphorus chloride, sucrose three by withLower processing 1～4 mixes (following weight g is 100% sterling quality), plastic bag sealing packaging, and shady and cool lucifuge place preserved after 1 year,Unpack, each processing is dissolved in respectively in 10L water, and short-tube lycoris kind ball is processed. The adjusting control agent having mixed by belowConcentration is watered 10L and fully dissolves and mix, and puts into plastic tub or Plastic Drum, after being watered, will now cash use; Soak bulb and root system 5～8min; When immersion, can not be placed on the place of direct light, should be placed on the place without direct light under tree shade or in cool canopy and soakBubble. After having soaked, take out planting ball, the density that is respectively 10cm, 20cm with seeding row spacing is planted in the medium fertilizer in its proving ground againIn the smooth soil block of force level, a kind ball of every root and stem of certain plants kind, conventional rich water quality management and the prevention and control of plant diseases, pest control. 12nd month time, gather in the crops,The bulbec number that its bulb mother bulb is tillered is counted and (diameter is reached to the above big or small bulbec number of tillering of 1cm and count, directlyFootpath is less than 1cm person and does not calculate therein); Occur on bulbec chassis fibrous root number is counted (with from mother bulbThe fibrous root occurring on bulbec chassis after taking off is as the criterion, and is less than long not the calculating therein of 0.5cm).
Test arranges 4 processing (1 contrast), and 3 repetitions of each processing, comprise contrast (6m2/ repeat). Sucrose is from lowTo height, 3 kinds of concentration gradients are set. Each Drug level of processing and compatibility situation are following, and (the following processing grams of getting all refers to100% sterling quality is dissolved in the amount in 10L water, i.e. concentration): processing 1:2.0g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.0g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride+0.5g sucrose; Process 2:2.2g6-benzyl amino-9-(tetrahydrochysene pyrroleMutter-2-yl)-9H-purine+1.3g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride+2.0g sucrose; Processing 3:2.5g6-benzyl amino-9-(oxinane-2-yl)-9H-purine+1.5g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride+3.0g sucrose; Process 4:2.2g6-Benzyl amino-9-(oxinane-2-yl)-9H-purine+1.3g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride; Contrast (CK): use certainlyCarry out water spray.
3.2 statistical analysis
Test data is adopted to SAS(6.12) carry out statistical analysis.
Process the chemical regulation effect of 12 months (i.e. 1 growth cycle) rear short-tube lycoris in table 3. Result of the test shows, in sprayAfter medicine 12 months (i.e. 1 growth cycle), the medicament of different disposal is deposited after 1 year and is only omited than the regulating effect of now with the current (table 2)Have decline, this explanation auxiliary agent sucrose has stabilization; By contrast, overground part and the underground part of processing 2 are all high for different disposalProcess in other, regulation and control synergy the best of processing 2 is described, 2.2g6-benzyl amino-9-(oxinane-2-yl)-9H-is fastThe regulation and control cooperative effect of purine+1.3g tributyl (the chloro-2-thiophene of 5-) phosphorus chloride+2.0g sucrose is positive-effect, and its regulating effectTo get well compared with other processing.
Table 3. is processed the chemical regulation effect of rear short-tube lycoris 12 months (1 bulb is tillered the cycle)
Result of the test shows, with not processing 4 comparisons of adding assistant sucrose, processes 0.5～3.0g auxiliary agent sucrose pair of 1～3The preservation of 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is all favourable; NoAdding assistant sucrose (processing 4), its regulating effect significantly reduces. So, needing adding assistant sucrose, its shelf-life is 1 year. In adjusting control agentSucrose mainly as auxiliary agent, play preparation filling and stable.
More than test shows, 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-)Phosphorus chloride all has short effect of tillering preferably. 6-benzyl amino-9-(oxinane-2-yl)-9H-purine is mainly to activate stoneGarlic bud primordial cell, promotes its cell to accelerate division, forms the former base of new bud; And tributyl (the chloro-2-thiophene of 5-) phosphorus chloride toolHave and can promote the synthetic of cell DNA, accelerate its division. So, 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and threeButyl (the chloro-2-thiophene of 5-) phosphorus chloride all has short effect of tillering preferably, and can stimulate the formation of the former base of root and stretchLong, there is the effect of growth-promoting root. Kinetin belongs to the basic element of cell division, though tool have certain effect, with 6-benzyl amino-9-(fourHydrogen pyrans-2-yl)-9H-purine, tributyl (the chloro-2-thiophene of 5-) phosphorus chloride compare, and its effect is not as good as 6-benzyl amino-9-(tetrahydrochysenePyrans-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chloride. NAA belongs to auxins, but to the former base of short-tube lycoris budFormation with its effect of growth also not as good as 6-benzyl amino-9-(oxinane-2-yl)-9H-purine and tributyl (the chloro-2-thiophene of 5-Fen) phosphorus chloride. Though NAA and indolebutyric acid are commonly used for root-growing agent, its physio-biochemical characteristics of different plants are not exclusively the same, instituteWith, to the effective conditioning agent of some plants but to its action effect possibility difference of other plants, so, wideless in theoryThe root-growing agent of adaptive. In addition, its exercising result of any plant growth regulator variable concentrations may be completely different, high concentration and lowIts exercising result of the plant growth regulator of concentration may be completely contrary.
So, auxiliary agent sucrose: 6-benzyl amino-9-(oxinane-2-yl)-9H-purine: tributyl (the chloro-2-thiophene of 5-))The mass ratio of phosphorus chloride is that the ingredients 2.5-3.5g of 2.0:2.2:1.3 is watered the formation of 10L to the former base of short-tube lycoris bud, the generation of tilleringBetter with the regulating effect that root occurs, the two mixing and there is the collaborative positive-effect of good regulation and control. This adjusting control agent produces short-tube lycorisProduct have the effect that improves quality, to soil also noresidue, are a kind of short-tube lycoris bulb tillering accelerators preferably.
Detailed description of the invention
Following embodiment and embodiment are intended to further illustrate the present invention, instead of limitation of the present invention.
Short-tube lycoris bulb tillering accelerator 6-benzyl amino-9-(oxinane-2-yl)-9H-purine: tributyl (the chloro-2-of 5-Thiophene) phosphorus chloride: the net content of sucrose mixes in the ratio of 2.0～2.5:1.0～1.5:0.5～3.0, preferably 2.2:1.3:2.0, plastic bag sealing packaging, shady and cool dry lucifuge place preserves 1 year. Sucrose, 6-benzyl amino-9-(oxinane-2-yl)-9H-The pure percentage composition of purine and tributyl (the chloro-2-thiophene of 5-) phosphorus chloride does not have particular/special requirement, and it is steady not affecting for its impurityUnder prerequisite, when compatibility, reach the desired net content of formula qualitatively.
Using method: the adjusting control agent of preserving 1 year mixing is in mass ratio referred to 100% is pure by every 2.5～3.5g(The net content of degree) be watered 10L(10kg), fully dissolve and mix, put into plastic tub or Plastic Drum, after being watered, to now cash use;Soak bulb and root system 5～8min; When immersion, can not be placed on the place of direct light, should be placed under tree shade or in cool canopy without direct projectionSoak in the place of light. After having soaked, take out planting ball, then be planted in soil block.
Embodiment 1
2.5g mixture be watered 10.0kg regulating effect [sucrose: 6-benzyl amino-9-(oxinane-2-yl)-9H-purine:The mass ratio of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is 2.0:2.2:1.3]:
Test is carried out in Institutes Of Technology Of Changsha's Jiuyi Shan Mountain proving ground. Excavate short-tube lycoris bulb kind from Jiuyi Shan Mountain proving groundBall, the adjusting control agent of preserving 1 year that 2.5g is mixed is in mass ratio watered 10L and fully dissolves and mix, put into plastic tub orIn Plastic Drum, after being watered, to now cash use; Soak bulb and root system 5～8min; When immersion, can not be placed on the place of direct light,Should be placed on the place without direct light under tree shade or in cool canopy soaks. After having soaked, take out planting ball, be respectively with seeding row spacingThe density of 10cm, 20cm is planted in the smooth soil block of its proving ground medium fertility level again, a kind ball of every root and stem of certain plants kind, routineRich water quality management and the prevention and control of plant diseases, pest control. 12nd month time, gather in the crops, it is (right that the bulbec number that its bulb mother bulb is tillered is countedDiameter reaches the bulbec of tillering of the above size of 1cm to be counted, and diameter is less than 1cm person and does not calculate therein); To on bulbec chassisThe fibrous root occurring counts that (fibrous root occurring with bulbec chassis after mother bulb is taken off is as the criterion, and is less thanWhat 0.5cm was long does not calculate therein).
6-benzyl amino-9-(oxinane-2-yl)-9H-purine: tributyl (the chloro-2-thiophene of 5-) phosphorus chloride: sucrose threeTo mix (reagent is all by 100% sterling quality) by 2.2:1.3:2.0, plastic bag sealing packaging, shady and cool lucifuge place preserves 1Nian Hou, unpacks, and is dissolved in 10L water, and short-tube lycoris bulb is processed. Process 12 months (i.e. 1 growth cycle) rear every113.67 of the number average out to of tillering of 100 root and stem of certain plants short-tube lycoris, have more 69.76 than 43.91 of contrasts, and short tillering capacity is 158.87%;The fibrous root number occurring on bulbec chassis is 19.61, has more 10.89 than 8.72 of contrasts, and short root incidence is124.89%。
Embodiment 2
3.0g mixture be watered 10.0kg regulating effect [sucrose: 6-benzyl amino-9-(oxinane-2-yl)-9H-purine:The mass ratio of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is 2.0:2.2:1.3]:
Test is carried out in Institutes Of Technology Of Changsha's Jiuyi Shan Mountain proving ground. Excavate short-tube lycoris bulb kind from Jiuyi Shan Mountain proving groundBall, the adjusting control agent of preserving 1 year that 3.0g is mixed is in mass ratio watered 10L and fully dissolves and mix, put into plastic tub orIn Plastic Drum, after being watered, to now cash use; Soak bulb and root system 5～8min; When immersion, can not be placed on the place of direct light,Should be placed on the place without direct light under tree shade or in cool canopy soaks. After having soaked, take out planting ball, be respectively with seeding row spacingThe density of 10cm, 20cm is planted in the smooth soil block of its proving ground medium fertility level again, a kind ball of every root and stem of certain plants kind, routineRich water quality management and the prevention and control of plant diseases, pest control. 12nd month time, gather in the crops, it is (right that the bulbec number that its bulb mother bulb is tillered is countedDiameter reaches the bulbec of tillering of the above size of 1cm to be counted, and diameter is less than 1cm person and does not calculate therein); To on bulbec chassisThe fibrous root occurring counts that (fibrous root occurring with bulbec chassis after mother bulb is taken off is as the criterion, and is less thanWhat 0.5cm was long does not calculate therein).
6-benzyl amino-9-(oxinane-2-yl)-9H-purine: tributyl (the chloro-2-thiophene of 5-) phosphorus chloride: sucrose threeTo mix (reagent is all by 100% sterling quality) by 2.2:1.3:2.0, plastic bag sealing packaging, shady and cool lucifuge place preserves 1Nian Hou, unpacks, and is dissolved in 10L water, and short-tube lycoris bulb is processed. Process 12 months (i.e. 1 growth cycle) rear every135.69 of the number average out to of tillering of 100 root and stem of certain plants short-tube lycoris, have more 91.78 than 43.91 of contrasts, and short tillering capacity is 209.02%;The fibrous root number occurring on bulbec chassis is 22.94, has more 14.22 than 8.72 of contrasts, and short root incidence is163.07%。
Embodiment 3
3.5g mixture be watered 10.0kg regulating effect [sucrose: 6-benzyl amino-9-(oxinane-2-yl)-9H-purine:The mass ratio of tributyl (the chloro-2-thiophene of 5-) phosphorus chloride is 2.0:2.2:1.3]:
Test is carried out in Institutes Of Technology Of Changsha's Jiuyi Shan Mountain proving ground. Excavate short-tube lycoris bulb kind from Jiuyi Shan Mountain proving groundBall, the adjusting control agent of preserving 1 year that 3.5g is mixed is in mass ratio watered 10L and fully dissolves and mix, put into plastic tub orIn Plastic Drum, after being watered, to now cash use; Soak bulb and root system 5～8min; When immersion, can not be placed on the place of direct light,Should be placed on the place without direct light under tree shade or in cool canopy soaks. After having soaked, take out planting ball, be respectively with seeding row spacingThe density of 10cm, 20cm is planted in the smooth soil block of its proving ground medium fertility level again, a kind ball of every root and stem of certain plants kind, routineRich water quality management and the prevention and control of plant diseases, pest control. 12nd month time, gather in the crops, it is (right that the bulbec number that its bulb mother bulb is tillered is countedDiameter reaches the bulbec of tillering of the above size of 1cm to be counted, and diameter is less than 1cm person and does not calculate therein); To on bulbec chassisThe fibrous root occurring counts that (fibrous root occurring with bulbec chassis after mother bulb is taken off is as the criterion, and is less thanWhat 0.5cm was long does not calculate therein).
6-benzyl amino-9-(oxinane-2-yl)-9H-purine: tributyl (the chloro-2-thiophene of 5-) phosphorus chloride: sucrose threeTo mix (reagent is all by 100% sterling quality) by 2.2:1.3:2.0, plastic bag sealing packaging, shady and cool lucifuge place preserves 1Nian Hou, unpacks, and is dissolved in 10L water, and short-tube lycoris bulb is processed. Process 12 months (i.e. 1 growth cycle) rear every103.84 of the number average out to of tillering of 100 root and stem of certain plants short-tube lycoris, have more 59.93 than 43.91 of contrasts, and short tillering capacity is 136.48%;The fibrous root number occurring on bulbec chassis is 17.91, has more 9.19 than 8.72 of contrasts, and short root incidence is105.39%。
Claims (2)
1. a short-tube lycoris bulb tillering accelerator, is characterized in that, described short-tube lycoris bulb tillering accelerator is by 6-benzyl amino-9-The matter that (oxinane-2-yl)-9H-purine, tributyl (the chloro-2-thiophene of 5-) phosphorus chloride and auxiliary agent sucrose are pressed 2.2:1.3:2.0Amount is than mixing, and plastic bag sealing is packed, and shady and cool dry lucifuge place preserves, 1 year shelf-life.
2. a using method for short-tube lycoris bulb tillering accelerator as claimed in claim 1, is characterized in that, plants short-tube lycorisTime, the adjusting control agent having mixed is watered to 10L by every 2.5～3.5g and fully dissolves after mixing and soak, after being watered, to now convertNow use, soaks bulb kind ball bottom and root system 5～8min, can not be placed on the place of direct light when immersion, should be placed under tree shadeOr soak without the place of direct light in cool canopy.
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