CN104328143A - 一种鲜烟叶生产酒精的方法 - Google Patents
一种鲜烟叶生产酒精的方法 Download PDFInfo
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- 241000208125 Nicotiana Species 0.000 title claims abstract description 40
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 40
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 239000000843 powder Substances 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 10
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 5
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 5
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
- 108010059892 Cellulase Proteins 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 230000007062 hydrolysis Effects 0.000 claims description 6
- 238000006460 hydrolysis reaction Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 238000010009 beating Methods 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 239000001913 cellulose Substances 0.000 abstract description 6
- 229920002678 cellulose Polymers 0.000 abstract description 6
- 238000001816 cooling Methods 0.000 abstract 2
- 108010093305 exopolygalacturonase Proteins 0.000 abstract 2
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 238000004537 pulping Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 235000000346 sugar Nutrition 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000002699 waste material Substances 0.000 description 3
- 235000019504 cigarettes Nutrition 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005903 acid hydrolysis reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 235000021180 meal component Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
本发明提供了一种鲜烟叶生产酒精的方法，具体如下：鲜烟叶，破碎，打浆，加入干烟叶粉20%、果胶酶0.15%、纤维素酶0.1%，混合均匀，50℃，酶解90min，加入盐酸2%，140-145℃水解60min，冷却至80℃，离心，得上清液；加入硫酸铵0.1%、磷酸二氢钾0.1%、硫酸镁0.05%，调pH值至5.0-5.5，85℃杀菌5min，冷却至30℃，接种酒精活性干酵母0.1%、果胶酶0.1%、纤维素酶0.05%，28-32℃发酵60h，在发酵至24h、40h时分别添加干烟叶粉10%、8%，蒸馏，即得酒精。
Description
技术领域
本发明属于化工领域，涉及一种鲜烟叶生产酒精的方法。
背景技术
烟草是我国农业经济的主导产业之一。据统计，全国烟草种植面积达1500多万亩，除去可用于深加工的正常烟叶外，还剩有烟秆、烟梗、烟花、烟杈、底脚叶和部分顶叶等数量巨大的烟草废弃物，其中废弃鲜烟叶每年约有180万吨。废弃鲜烟叶含有碳水化合物4-5%、还原糖2.5-3%，纤维素1.5-2%，还有少量的蛋白质、烟碱及挥发酸、矿物质等，废弃了十分可惜，不但浪费了资源，有时还会造成环境污染。
发明内容
本发明提供了一种鲜烟叶生产酒精的方法。
本发明的目的是按下述方式实现的：鲜烟叶，破碎，打浆，加入干烟叶粉20%、果胶酶0.15%、纤维素酶0.1%，混合均匀，50℃，酶解90min，加入盐酸2%，140-145℃水解60min，冷却至80℃，离心，得上清液；加入硫酸铵0.1%、磷酸二氢钾0.1%、硫酸镁0.05%，调pH值至5.0-5.5，85℃杀菌5min，冷却至30℃，接种酒精活性干酵母0.1%、果胶酶0.1%、纤维素酶0.05%，28-32℃发酵60h，在发酵至24h、40h时分别添加干烟叶粉10%、8%，发酵结束后进行蒸馏，即得酒精。
所述的干烟叶粉是指鲜烟叶经烘干、粉碎、过40目筛制得的干烟叶粉。
经过检测，鲜烟叶成分如下：水分85%、水溶性总糖4.48%、还原糖3.98%、纤维素1.87%、蛋白质0.08%、总氮0.03%；干烟叶粉成分如下：水溶性总糖29.85%、还原糖26.50%、纤维素12.46%、蛋白质0.92%、总氮0.37%。由成分分析可以见，鲜烟叶中含有可发酵糖、蛋白质和氨基酸，但总糖含量偏低。为了提高生产效率和酒精收率，并充分利用烟叶资源，本发明将鲜烟叶经烘干、粉碎制成干烟叶粉，其水溶性总糖接近30%，再加回到配料中，解决了初始总糖偏低的问题；同时为了有效利用烟叶含有的纤维素，采用酶水解加酸水解的双重水解方式，来提高利用率。本发明为大量废弃烟叶的综合利用提供了一条有效途径。
四、具体实施例
实施例1
具体实施方式：称取鲜烟叶1000g，破碎，打浆，加入干烟叶粉200g、果胶酶1.5g、纤维素酶1g，混合均匀，50℃，酶解90min，加入盐酸20mL，140-145℃水解60min，冷却至80℃，离心，得上清液；量取上清液500mL，加入硫酸铵0.5g、磷酸二氢钾0.5g、硫酸镁0.25g，调pH值至5.0-5.5，85℃杀菌5min，冷却至30℃，接种酒精活性干酵母0.5g、果胶酶0.5g、纤维素酶0.25g，28-32℃发酵48h，发酵24h时添加干烟叶粉50g，发酵至40h时再添加干烟叶粉40g，发酵结束后进行蒸馏，即得酒精。
实施例2
具体实施方式：称取鲜烟叶500g，鲜烟叶，破碎，打浆，加入干烟叶粉100g、果胶酶0.75g、纤维素酶0.5g，混合均匀，50℃，酶解90min，加入盐酸10mL，140-145℃水解60min，冷却至80℃，离心，得上清液；量取上清液200mL，加入硫酸铵0.2g、磷酸二氢钾0.2g、硫酸镁0.1g，调pH值至5.0-5.5，85℃杀菌5min，冷却至30℃，接种酒精活性干酵母0.2g、果胶酶0.2g、纤维素酶0.1g，28-32℃发酵60h，在发酵至24h、40h时分别添加干烟叶粉20g、16g，发酵结束后进行蒸馏，即得酒精。
Claims (1)
1.本发明提供了一种鲜烟叶生产酒精的方法，其特征如下：鲜烟叶，破碎，打浆，加入干烟叶粉20%、果胶酶0.15%、纤维素酶0.1%，混合均匀，50℃，酶解90min，加入盐酸2%，140-145℃水解60min，冷却至80℃，离心，得上清液；加入硫酸铵0.1%、磷酸二氢钾0.1%、硫酸镁0.05%，调pH值至5.0-5.5，85℃杀菌5min，冷却至30℃，接种酒精活性干酵母0.1%、果胶酶0.1%、纤维素酶0.05%，28-32℃发酵60h，在发酵至24h、40h时分别添加干烟叶粉10%、8%，发酵结束后进行蒸馏，即得酒精。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106811381A (zh) * | 2017-02-23 | 2017-06-09 | 湖北中烟工业有限责任公司 | 一种烟草可可酒新型烟草制品及其制备方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167828A (zh) * | 1996-12-11 | 1997-12-17 | 朱大恒 | 烟草工业专用酒精的生产方法 |
| WO2005118827A2 (en) * | 2004-05-27 | 2005-12-15 | Novozymes North America, Inc | Fermentation process |
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2014
- 2014-10-24 CN CN201410570864.7A patent/CN104328143A/zh active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167828A (zh) * | 1996-12-11 | 1997-12-17 | 朱大恒 | 烟草工业专用酒精的生产方法 |
| WO2005118827A2 (en) * | 2004-05-27 | 2005-12-15 | Novozymes North America, Inc | Fermentation process |
Non-Patent Citations (2)
| Title |
|---|
| ANDRIANOV, V ET AL.: "Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass", 《PLANT BIOTECHNOLOGY JOURNAL》 * |
| 谢丽萍等: "烟草下脚料发酵制取乙醇", 《环境工程学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106811381A (zh) * | 2017-02-23 | 2017-06-09 | 湖北中烟工业有限责任公司 | 一种烟草可可酒新型烟草制品及其制备方法 |
| CN106811381B (zh) * | 2017-02-23 | 2020-03-10 | 湖北中烟工业有限责任公司 | 一种烟草可可酒新型烟草制品及其制备方法 |
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