CN104324149B - Dysmenorrhea drug and preparation method thereof - Google Patents
Dysmenorrhea drug and preparation method thereof Download PDFInfo
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Abstract
It is a kind of dysmenorrhea drug and preparation method thereof, the raw material of the dysmenorrhea drug includes corydalis tuber, Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and root of Altai Daphne the present invention relates to Kazak medicine technical field.The dysmenorrhea is pressed with drug to be stated preparation method and obtains: the first step, the desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and root of Altai Daphne are mixed to form mixing medicinal material, steam distillation is carried out after water is added into mixing medicinal material, the volatile oil of aqueous solution and extraction is respectively obtained after steam distillation.Dysmenorrhea of the invention is made with drug utilization Kazak medicinal material, preparation method is simple, drug used is all warm nature drug, has the function of channel warming and blood nourishing, menstruction regulating and pain relieving, nontoxic pair, curative effect is short when treating dysmenorrhea using the dysmenorrhea drug obtained according to that above embodiment of the present invention, it is obvious to improve dysmenorrhea symptom, treated effect is high, has no toxic side effect, no drug resistance phenomenon, it is uneasy to recur after treatment, it can effectively be released for patient ailing.
Description
Technical field
It is a kind of dysmenorrhea drug and preparation method thereof the present invention relates to Kazak medicine technical field.
Background technique
Dysmenorrhea, or be menstrual period pain, it is one of most common symptom of gynecological patient.Refer to women's positive value menstrual period or through row
There is periodical underbelly pain in front and back or pain draws waist sacrum or even severe pain syncope person, also known as " dysmenorrhea ".Clinically by dysmenorrhea point
For primary dysmenorrhea and acquired dysmenorrhea.Primary dysmenorrhea refers to that functional dysmenorrhea, acquired dysmenorrhea are by the organic disease of pelvic cavity
Caused by change.Many women have slight discomfort in menstrual period, but dysmenorrhea refers to the normal activity of the ache influence in menstrual period, and
Need drug therapy.Periodic menstrual period pain is common and betides most of menstrual cycles.Pain is often colic pain simultaneously
With back pain, Nausea and vomiting, headache or diarrhea.26% puberty women is since dysmenorrhea cannot go to school or go to work.Typically
It is that pain occurs at first day of menstruation, the time usually started in menstruation, but also someone starts to ache until the second talent of menstruation
Bitterly.Pain is in spastic, paroxysmal.When serious complexion whiten, be in a cold sweat, general weakness, peripheral coldness.Nausea,vomiting,diarrhea
It is also more typical with having a headache.Patients ' psychological and physiology are affected greatly, are easy to induce the other diseases of itself.Western medical treatment pain
Through analgesic drug products such as common Fenbids, but side effect is big.Often cause symptom of digestive tract include indigestion, stomach burn feeling, stomachache,
There is gastric ulcer and hemorrhage of digestive tract in Nausea and vomiting etc., small number of patients, also have because of perforated ulcer person.It may occur in which nervous system disease
Shape such as has a headache, is drowsiness, is dizzy.Brufen, which is used for a long time, can cause kidney function damage, and brufen has inhibition to platelet aggregation
Effect, can make prolonged bleeding time, and causing bleeding has hepatic disfunction, vitamin K deficiency, hemophilia and other blood clottings barrier
Hinder, or because the diseases such as gastric and duodenal ulcer, ulcerative colitis using brufen is taken after anti-coagulants again, can cause to disappear
Change road recessive bleeding, hematochezia, hematuria, wound blood oozing from the wound surface or ecchymosis can occur for severe one.The person of suffering with chronic bronchitis takes
Delayed bronchial asthma easily occurs after brufen.Allergic rash can also be caused.Although using can be relieved pain after brufen,
But it cannot cure, be easy recurrence.Brufen analgesic, which is used for a long time, can cause drug resistance.
Doctor trained in Western medicine also treats dysmenorrhea with hormone medicine, and side effect is larger, can there is Nausea and vomiting, giddy, out of strength, drowsiness etc.
Class morning sickness and irregular bleeding；Occasionally there are spargosis, fash, acne, weight gain etc..It cannot miss, miss and may occur in which yin
It can be pretty troublesome during road is bled profusely, therefore patient takes.There are liver, nephrosis to avoid use.Fibroid, hypertensive patient and there are liver, kidney
Medical history person is used with caution.May occur in which central obesity, moon shaped face, purple striae, thinning of skin, myasthenia, muscular atrophy, Diagnostic value, edema,
Nausea and vomiting, hypertension, glycosuria, acne, crinosity, infection, pancreatitis, poor wound healing, osteoporosis, induction or exacerbation
The side effects such as digestive tract ulcer.Prolonged application easily causes to infect, and more typical infection is the sense such as respiratory tract, skin, urinary tract
Dye and tuberculosis etc..Large dosage of hormone can cause blood leukocytes to increase, sometimes up to 20 × 109/L.Large dosage of hormone can cause
Diuresis.But when head begins treatment, hormone still fails to play diuresis, can cause water, sodium retention instead, aggravates oedema,
At this time if it is necessary, diuretics should be used simultaneously, and carry out low salt diet.Hormone aggravates sometimes or causes hypertension, should
With Treatment of Hypertension.After there is diuresis, it should be noted that have the possibility of hypopotassaemia, patient should be advised suitably to feed the food abundant containing potassium
Object, or isokalaemic diuretic is shared, such as still there is Diagnostic value, can suitably supplement sylvite.Hormone can increase calcium, phosphorus excretion, reduce calcium
Absorption, it is long-term to widely apply, osteoporosis, spontaneous fracture and aseptic necrosis of femoral head can be caused.Can easily cause excitement,
Insomnia, can not induce mental disease, and stable equal sedatives can be suitably used.Hormone can promote breaks down proteins and its synthesis is inhibited to make
At negative nitrogen balance, therefore high-protein diet is preferably given, some patients originally had slight azotemia, aggravated after hormone therapy, very
To there is renal failure.Hormone can promote gluconeogenesis, reduce utilization of the tissue to glucose, and serious person can occur blood glucose and increase and sugar
Urine.Prolonged application hormone can induce cataract, poor wound healing, thrombosis and embolism disease, hidrosis and night sweat,
And menstrual disorder etc..Therefore apply hormone medicine treatment dysmenorrhea should be prudent.
Chinese medicine application oral medicinal herb and herbal enema the external therapys treatment dysmenorrhea such as stick and have many shortcoming places, and Chinese medicine is protected
The external therapys complex treatment such as bowel lavage is stayed, patient is unable to Self-operating, it is necessary to treat to Implementing Hospital, make troubles, make to patient
Patient cannot adhere to the treatment of specification, and influence therapeutic effect.It can decline using Chinese medicine external patient compliance for a long time, more ways
Diameter administration is effective, but treatment method is more cumbersome, and patient is not easy to adhere to.It takes for a long time using Chinese medicine for severe dysmenorrhea patient,
Therapeutic effect owes ideal sometimes.Prolonged not off-drug, the compliance of patient can be gradually reduced.The course of disease of traditional Chinese medicine treatment dysmenorrhea
Length, the course for the treatment of are long, improve slow.Dysmenorrhea Patients on Recurrence rate is high, and treatment late result owes satisfaction, and curative effect cannot consolidate or even Cheng Gu
Disease, so prevention and reduction recurrence are the key that treatments.To the altauna warp or duration pelvic pain caused by being in a bad way
Patient, traditional Chinese medicine treatment still effectively or cannot relieve pain completely, need to cooperate Western medicine analgestic, or even use operative treatment.
Summary of the invention
The present invention provides a kind of dysmenorrhea drugs and preparation method thereof, overcome the defects of the prior art, energy
Effectively solve the problems, such as current traditional Chinese medicine treatment dysmenorrhea there are the courses for the treatment of it is long, improve slowly, side effect is big, treatment method is more loaded down with trivial details.
Technical solution of the present invention first is that being realized by following measures: a kind of dysmenorrhea drug, raw material is by weight
Amount number meter include 3 parts to 10 parts of corydalis tuber, 6 parts to 12 parts of Radix Angelicae Sinensis, 3 parts to 10 parts of Rhizoma Chuanxiong, 3 parts to 9 parts of folium artemisiae argyi, 6 parts of the root of three-nerved spicebush extremely
10 parts, 3 parts to 6 parts and 0.5 part to 1 part of root of Altai Daphne of fennel seeds.
Here is further optimization or/and improvement of one of the above technical solutions of the invention:
Above-mentioned dysmenorrhea is obtained as follows with drug: the first step, by the desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, small
Fennel and root of Altai Daphne mix to form mixing medicinal material, and mixing quality of medicinal material 6 is added into mixing medicinal material again to 9 times
Steam distillation is carried out after water, the temperature of steam distillation is 100 DEG C to 200 DEG C, the time is 4 hours to 6 hours, and vapor steams
The volatile oil of aqueous solution and extraction is respectively obtained after evaporating；Second step, to the desired amount of corydalis tuber progress refluxing extraction 1 time to 3 times, often
Secondary refluxing extraction be added into corydalis tuber corydalis tuber quality 4 again to 10 times volumetric concentration be 30% to 95% ethanol water, every time
The temperature of refluxing extraction is 60 DEG C to 100 DEG C, the time is 0.5 hour to 3.5 hours, and filter is obtained by filtration after each refluxing extraction
Liquid obtains mixed filtrate after merging filtrate, recycles the ethyl alcohol in mixed filtrate, and the mixed filtrate and the first step after recycling ethyl alcohol are returned
Stream extracts obtained aqueous solution and merges to obtain mixed solution, aqueous by quality is obtained after mixed solution concentration, vacuum drying
The dry extract that amount is 1% to 10%, is broken into 100 mesh to 200 targeted fine powders for dry extract；Fine powder is added into fine powder for third step
Quality 1 obtains mixing fine powders to the sweetener of 3 times of dextrin, fine powder quality 0.1% to 5% again, the volatile oil obtained to the first step
Middle addition volatilization oil quality 0.5 is diluted to obtain to dilute to the ethanol water that 2 times of volumetric concentration is 80% to 95% again waves
Mixing fine powders and dilution volatile oil are made particle after mixing, obtain dysmenorrhea drug after dry, whole grain by hair oil.
The number of refluxing extraction is 2 times in above-mentioned second step；Or/and in second step, the ethyl alcohol in recycling mixed filtrate is extremely
The mass content of ethyl alcohol is less than 10% in mixed filtrate.
Concentration is is concentrated under reduced pressure in above-mentioned second step, and the temperature of reduced pressure is 50 DEG C to 80 DEG C, pressure is -0.05MPa
To -0.09MPa；Or/and vacuum drying temperature is 50 DEG C to 80 DEG C in second step, pressure is -0.05MPa to -0.09MPa.
In above-mentioned third step, mixing fine powders and dilution volatile oil are constructed to after mixing through 10 meshes, Bu Nengtong
Cross the particle of 80 meshes；It is 0.5% to 5% by particle drying to granular mass water content or/and in third step；Or/and third
Particle in step after drying carries out whole grain can pass through 10 meshes, cannot pass through 80 meshes.
Technical solution of the present invention second is that being realized by following measures: a kind of dysmenorrhea drug and its preparation side
Method carries out in the steps below: the first step mixes the desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and root of Altai Daphne
It is combined to form mixing medicinal material, mixing quality of medicinal material 6 is added into mixing medicinal material again to progress vapor steaming after 9 times of water
It evaporates, the temperature of steam distillation is 100 DEG C to 200 DEG C, the time is 4 hours to 6 hours, is respectively obtained after steam distillation water-soluble
The volatile oil of liquid and extraction；Second step, carries out the desired amount of corydalis tuber refluxing extraction 1 time to 3 times, and each refluxing extraction is to corydalis tuber
Middle that corydalis tuber quality 4 is added again to the ethanol water that 10 times of volumetric concentration is 30% to 95%, the temperature of each refluxing extraction is
60 DEG C to 100 DEG C, the time be 0.5 hour to 3.5 hours, filtrate is obtained by filtration after each refluxing extraction, is obtained after merging filtrate
Mixed filtrate recycles the ethyl alcohol in mixed filtrate, and the mixed filtrate after recycling ethyl alcohol obtains water-soluble with first step refluxing extraction
Liquid merges to obtain mixed solution, will obtain the dry leaching that mass water content is 1% to 10% after mixed solution concentration, vacuum drying
Dry extract is broken into 100 mesh to 200 targeted fine powders by cream；Fine powder quality 1 is added into fine powder again to 3 times of paste for third step
Smart, fine powder quality 0.1% to 5% sweetener obtains mixing fine powders, and volatilization oil quality is added into the volatile oil that the first step obtains
The ethanol water that 0.5 times to 2 times of volumetric concentration is 80% to 95% is diluted to obtain dilution volatile oil, by mixing fine powders and
Particle is made in dilution volatile oil after mixing, obtains dysmenorrhea drug after dry, whole grain；Wherein, the raw material of dysmenorrhea drug
It according to parts by weight include 3 parts to 10 parts of corydalis tuber, 6 parts to 12 parts of Radix Angelicae Sinensis, 3 parts to 10 parts of Rhizoma Chuanxiong, 3 parts to 9 parts of folium artemisiae argyi, the root of three-nerved spicebush 6
Part is to 10 parts, 3 parts to 6 parts and 0.5 part to 1 part of root of Altai Daphne of fennel seeds.
Here is further optimization or/and improvement of the technical scheme ii of the invention:
The number of refluxing extraction is 2 times in above-mentioned second step；Or/and in second step, the ethyl alcohol in recycling mixed filtrate is extremely
The mass content of ethyl alcohol is less than 10% in mixed filtrate.
Concentration is is concentrated under reduced pressure in above-mentioned second step, and the temperature of reduced pressure is 50 DEG C to 80 DEG C, pressure is -0.05MPa
To -0.09MPa；Or/and vacuum drying temperature is 50 DEG C to 80 DEG C in second step, pressure is -0.05MPa to -0.09MPa.
In above-mentioned third step, mixing fine powders and dilution volatile oil are constructed to after mixing through 10 meshes, Bu Nengtong
Cross the particle of 80 meshes；It is 0.5% to 5% by particle drying to granular mass water content or/and in third step；Or/and third
Particle in step after drying carries out whole grain can pass through 10 meshes, cannot pass through 80 meshes.
Dysmenorrhea of the invention is made with drug utilization Kazak medicinal material, and preparation method is simple, and drug used is all warm nature
Drug is had the function of channel warming and blood nourishing, menstruction regulating and pain relieving, nontoxic pair, is used using the dysmenorrhea obtained according to that above embodiment of the present invention
Drug curative effect when treating dysmenorrhea is short, and improvement dysmenorrhea symptom is obvious, and treated effect is high, has no toxic side effect, no drug resistance phenomenon is controlled
It is uneasy to recur after treatment, it can effectively be released for patient ailing.
Specific embodiment
The present invention is not limited by the following examples, can determine according to the technique and scheme of the present invention with actual conditions specific
Embodiment.Unless stated otherwise, the % in the present invention is mass percent；Unless stated otherwise, preparation process is normal
It is carried out under temperature, normal pressure state；Unless stated otherwise, reagent, the method and apparatus used in the present invention is conventional for the art
Reagent, method and apparatus；Unless stated otherwise, agents useful for same is commercially available in the present invention；Unless stated otherwise, in the present invention
Water is deionized water；Unless stated otherwise, the solution in the present invention is the aqueous solution that solvent is water, for example, if not doing especially
Illustrate, hydrochloric acid solution is aqueous hydrochloric acid solution.
The present invention will be further described below with reference to examples:
Embodiment 1, the raw material of the dysmenorrhea drug include 3 parts to 10 parts of corydalis tuber, 6 parts to 12 of Radix Angelicae Sinensis according to parts by weight
0.5 part of part, 3 parts to 10 parts of Rhizoma Chuanxiong, 3 parts to 9 parts of folium artemisiae argyi, 6 parts to 10 parts of the root of three-nerved spicebush, 3 parts to 6 parts of fennel seeds and root of Altai Daphne are extremely
1 part.The present embodiment is according to pharmacopeia or this field conventional phamaceutical techniques means also available dysmenorrhea drug.It is of the present invention
Member is acted like a bully the dry tuber of papaveraceae plant corydalis Corydalis yanhusuo W.T.Wang.When being classified as umbelliferae
Angelica sinensis(Oliv.) Diels dry root.Rhizoma Chuanxiong is umbelliferae Rhizoma Chuanxiong Ligusticum
The dry rhizome of chuanxiong Hort..Folium artemisiae argyi is the drying of compositae plant Chinese mugwort Artemisia argyi Levl.et Vant
Leaf.Root of three-nerved spicebush lauraceae plant root of three-nerved spicebush Lindera aggregata(Sims) Kosterm. fast drying root.Fennel seeds are Umbelliferae plant
The dry mature fruit of object fennel Foeniculum vulgare Mill..Winter daphne is Isolated From Thymelaeaceae Species Altay winter daphne Daphne
The drying Pi Heye of altaica Pall.Wherein corydalis tuber is pungent, bitter, and temperature has effects that regulating qi-flowing for relieving pain, is clinically used for treating all kinds of
Pain, especially dysmenorrhea；The Radix Angelicae Sinensis sweet, acrid, warm, have dispelling wind and eliminating dampness, eliminating cold to stop pain, tonifying blood and regulating menstruation, the function of ease constipation,
It is clinically used for treatment dysmenorrhea, irregular menstruation, deficiency of blood amenorrhoea, anaemia, constipation, the diseases such as rheumatism；The Rhizoma Chuanxiong is acrid in flavour and warm in nature, has
Blood-activating and qi-promoting, the function of wind-expelling pain-stopping are clinically used for treatment dysmenorrhea, irregular menstruation, postpartum stasis abdominal pain, headache, rheumatism etc.
Disease；The folium artemisiae argyi is bitter, tepor, has dispelling cold and removing dampness, the function of warming channel for arresting bleeding is clinically used for irregular menstruation, and dysmenorrhea is functional
Uterine hemorrhage, the diseases such as amenorrhoea；The root of three-nerved spicebush is acrid in flavour and warm in nature, has and has a respite, middle benefit gas, cold dispelling, and the function of analgesic is clinically used for treating
A variety of pain；The fennel seeds are acrid in flavour and warm in nature, have qi-regulating, cold dispelling, and the function of appetizing is clinically used for treatment dysmenorrhea, and stomach duct and abdomen is swollen
Bitterly, the diseases such as deficiency of food vomiting and diarrhoea；The Altay winter daphne is acrid in flavour and warm in nature, toxic.With inducing sweat and dispelling exogenous evils, cough-relieving apophlegmatic, the function of analgesic,
It is clinically used for treatment dysmenorrhea, tracheitis, anemofrigid cold, rheumatism, the diseases such as pain in waist and lower extremities.
Embodiment 2, the raw material of the dysmenorrhea drug include 3 parts or 10 parts of corydalis tuber, 6 parts of Radix Angelicae Sinensis or 12 according to parts by weight
0.5 part of part, 3 parts or 10 parts of Rhizoma Chuanxiong, 3 parts or 9 parts of folium artemisiae argyi, 6 parts or 10 parts of the root of three-nerved spicebush, 3 parts or 6 parts of fennel seeds and root of Altai Daphne or
1 part.The present embodiment is according to pharmacopeia or this field conventional phamaceutical techniques means also available dysmenorrhea drug.
Embodiment 3, as the preferred of above-described embodiment, which is pressed with drug states preparation method and obtains: the first step, will
The desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and root of Altai Daphne mix to form mixing medicinal material, to mixing medicine
Mixing quality of medicinal material 6 is added in material again to steam distillation is carried out after 9 times of water, the temperature of steam distillation is 100 DEG C to 200
DEG C, the time be 4 hours to 6 hours, the volatile oil of aqueous solution and extraction is respectively obtained after steam distillation；Second step, to required
The corydalis tuber of amount carries out refluxing extraction 1 time to 3 times, and corydalis tuber quality 4 is added into corydalis tuber again to 10 times of volume for each refluxing extraction
The ethanol water that concentration is 30% to 95%, the temperature of each refluxing extraction is 60 DEG C to 100 DEG C, the time is 0.5 hour to 3.5
Hour, filtrate is obtained by filtration after each refluxing extraction, mixed filtrate is obtained after merging filtrate, recycles the ethyl alcohol in mixed filtrate,
The aqueous solution that mixed filtrate and first step refluxing extraction after recycling ethyl alcohol obtain merges to obtain mixed solution, will mix molten
The dry extract that mass water content is 1% to 10% is obtained after liquid concentration, vacuum drying, and dry extract is broken into 100 mesh to 200 purposes
Fine powder；Third step is added fine powder quality 1 into fine powder and is mixed again to the sweetener of 3 times of dextrin, fine powder quality 0.1% to 5%
Close fine powder, into the volatile oil that the first step obtains be added volatilization oil quality 0.5 again to 2 times volumetric concentration be 80% to 95% second
Alcohol solution is diluted to obtain dilution volatile oil, and particle is made in mixing fine powders and dilution volatile oil after mixing, dry,
Dysmenorrhea drug is obtained after whole grain.
Embodiment 4, as the preferred of above-described embodiment, which is pressed with drug states preparation method and obtains: the first step, will
The desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and root of Altai Daphne mix to form mixing medicinal material, to mixing medicine
Steam distillation is carried out after the water of 6 times or 9 times of quality of medicinal material of mixing is added in material, the temperature of steam distillation is 100 DEG C or 200
DEG C, the time be 4 hours or 6 hours, the volatile oil of aqueous solution and extraction is respectively obtained after steam distillation；Second step, to required
The corydalis tuber of amount carries out refluxing extraction 1 time or 3 times, and 4 times or 10 times of corydalis tuber quality of volume is added into corydalis tuber for each refluxing extraction
The ethanol water that concentration is 30% or 95%, the temperature of each refluxing extraction is 60 DEG C or 100 DEG C, the time is 0.5 hour or 3.5
Hour, filtrate is obtained by filtration after each refluxing extraction, mixed filtrate is obtained after merging filtrate, recycles the ethyl alcohol in mixed filtrate,
The aqueous solution that mixed filtrate and first step refluxing extraction after recycling ethyl alcohol obtain merges to obtain mixed solution, will mix molten
The dry extract that mass water content is 1% or 10% is obtained after liquid concentration, vacuum drying, and dry extract is broken into 100 mesh or 200 purposes
Fine powder；1 times or 3 times of fine powder quality of dextrin is added into fine powder for third step, the sweetener of fine powder quality 0.1% or 5% is mixed
Fine powder is closed, the second that the volumetric concentration of 0.5 times or 2 times of oil quality of volatilization is 80% or 95% is added into the volatile oil that the first step obtains
Alcohol solution is diluted to obtain dilution volatile oil, and particle is made in mixing fine powders and dilution volatile oil after mixing, dry,
Dysmenorrhea drug is obtained after whole grain.
Embodiment 5, as the preferred of embodiment 3 and embodiment 4, the number of refluxing extraction is 2 times in second step；Or/and
In second step, the mass content of ethyl alcohol ethyl alcohol into mixed filtrate in mixed filtrate is recycled less than 10%.
Embodiment 6, as the preferred of embodiment 3, embodiment 4 and embodiment 5, concentration subtracts in second step to be concentrated under reduced pressure
The temperature of pressure concentration is 50 DEG C to 80 DEG C, pressure is -0.05MPa to -0.09MPa；Or/and vacuum drying temperature in second step
Degree is 50 DEG C to 80 DEG C, pressure is -0.05MPa to -0.09MPa.
Embodiment 7, it is in third step, mixing is thin as the preferred of embodiment 3, embodiment 4, embodiment 5 and embodiment 6
Powder and dilution volatile oil be constructed to after mixing by 10 meshes, cannot be by the particle of 80 meshes；Or/and in third step,
It is 0.5% to 5% by particle drying to granular mass water content；Or/and the particle in third step after drying is can pass through 10 mesh
Sieve cannot carry out whole grain by 80 meshes.
One, the dysmenorrhea obtained according to that above embodiment of the present invention cures the clinical observation of dysmenorrhea with medicine
1, clinical therapeutic efficacy is evaluated:
1.1 case selections: extracting 197 patients for meeting primary dysmenorrhea (sympotoms caused by cold factors) diagnostic criteria and be grouped at random, clinical
Cardiovascular and cerebrovascular, liver, kidney and disease in the blood system are excluded, is confirmed through gynecologial examination and B ultrasound without organic disease, wherein taking orally
The dysmenorrhea obtained according to that above embodiment of the present invention with drug 147, be of the present invention group, average age (17.0 ± 5) year, be averaged
The course of disease (2 ± 0.24) year；Oral ibuprofen capsule 50, is control group, average age (18.0 ± 6) year, average course of disease (1.6
± 0.37) year.Two groups of ages and the course of disease are examined through t, P > 0.05, no significant difference.
Preceding two groups of menstruations to be treated to compare, the control group menstrual cycle is averaged (22.0 ± 6.0) day, and the menstrual period 3~7 days,
Through measure it is medium, of the present invention group of menstrual cycle, menstrual period, through measure (P > 0.05) roughly the same with control group, two groups of menstrual onsets are 3
Year or more.
1.2 methods:
1.2.1 of the present invention group: the oral dysmenorrhea drug obtained according to that above embodiment of the present invention, it is daily 3 times oral, often
Secondary 10g, premenstrual 3 days of the moon start medication, and are used continuously 5 days, two weeks is as a treatment course, and 3 courses for the treatment of are used in conjunction.
1.2.2 control group: oral ibuprofen capsule, 1 time 1,3 times a day, the daystart before menstrual onset is taken,
It even served 3 days.
Treatment results are evaluated after 3 periods of two groups of treatments.And disobedience other drugs during advising patient to treat, menstrual period avoid
Raw food, smooth feelings will.
1.3 results:
Cure: bellyache in menstrual period symptom disappears；
Effective: bellyache in menstrual period symptom disappears substantially；
Effective: bellyache in menstrual period symptom does not disappear, but mitigates earlier above；
Invalid: bellyache in menstrual period symptom does not disappear, unchanged earlier above.
1, present invention group treatment results (see Tables 1 and 2)
2, control group treatment results (being shown in Table 3 and table 4)
Present invention group treatment 147 cure 76 after the treatment it can be seen from table 1, table 2 and table 3 and table 4, show
Effect 58, invalid 13, cure rate 51.70%, obvious effective rate 39.46%, total effective rate 91.16%；Control group treats 50, is controlling
After treatment, healing 24, effective 9, invalid 17, cure rate 48%, obvious effective rate 18%, total effective rate 66%, it can thus be seen that
The dysmenorrhea drug obtained according to that above embodiment of the present invention must compared to current common drug sweet smell in terms for the treatment of primary dysmenorrhea
Obtaining capsule has better therapeutic effect.
Two, acute toxicity test
1, purpose
Emmenagogue raw material powder is detected to 1 to 3 stomach-filling in animal (mouse, rat) 1 day, observes the toxic reaction of its appearance
Symptom measures LD50Or maximum dosage-feeding, the safety for long term toxication, the dose design of pharmacodynamic test and clinical application provide
Foundation.
Since the toxicity of the dysmenorrhea drug obtained according to that above embodiment of the present invention is lower, LD is not measured50, can only survey most
Big dosage is as defined in the related guideline of national Bureau of Drugs Supervision with the raw material that auxiliary material is not added.
So-called emmenagogue raw material powder/raw material powder is according to the above embodiment of the present invention 1 or the raw material proportioning of embodiment 2 below
Choose raw material after, wear into fine powder be uniformly mixed obtain.
So-called emmenagogue is the dysmenorrhea drug obtained according to the above embodiment of the present invention below.
So-called life below will be to mix according to the raw material of the above embodiment of the present invention 1 or embodiment 2.
2, medication
Female mice after fasting 16 hours is divided equally 2 groups by 2.1: negative control group, emmenagogue raw material powder group are filled with maximum
Stomach concentration 0.5g/ml, maximum stomach-filling volume 40ml/kg, it is interior for 24 hours to give 2 emmenagogue raw material powder solution of intragastric administration on mice, negative control
Group gives the distilled water of isometric(al), observes and records acute toxicity test in mice changes of weight, toxic reaction and death condition, small
Mouse acute toxicity test changes of weight the results are shown in Table 5；
Female rats after fasting 16 hours are divided equally 2 groups by 2.2: negative control group, emmenagogue raw material powder group are filled with maximum
Stomach concentration 0.5g/ml, maximum stomach-filling volume 20ml/kg, it is interior for 24 hours to give 3 emmenagogue raw material powder solution of rat oral gavage, negative control
Group gives the distilled water of isometric(al), observes and records rat acute toxicity test changes of weight, toxic reaction and death condition, greatly
Mouse acute toxicity test changes of weight the results are shown in Table 6.
3, result
3.1 emmenagogue raw material powders can measure mouse LD50, under maximum dosage-feeding 40g raw material powder/kg dosage, mouse 2 weeks
Interior posture, four limbs activity, breathing, hair color, two just, the equal Non Apparent Abnormality compared with the control group such as body weight increase.When off-test
By mouse gross anatomy, the equal Non Apparent Abnormality of the heart, liver, spleen, lung, kidney is visually observed；Mouse exists it can be seen from data in table 5
After taking emmenagogue raw material powder solution, the bodyweight difference of negative control group and emmenagogue raw material powder group is little.Illustrate according to this hair
The dysmenorrhea that bright above-described embodiment obtains is safety by acute toxicity test with drug.
3.2 emmenagogue raw material powders can measure rat LD50, under maximum dosage-feeding 30g raw material powder/kg dosage, rat 2
In week posture, four limbs activity, breathing, hair color, two just, the equal Non Apparent Abnormality compared with the control group such as body weight increase.Off-test
When by rat gross anatomy, visually observe the equal Non Apparent Abnormality of the heart, liver, spleen, lung, kidney；The rat it can be seen from data in table 6
After taking emmenagogue raw material powder solution, the bodyweight difference of negative control group and emmenagogue raw material powder group is little.Illustrate according to this
The dysmenorrhea that invention above-described embodiment obtains is safety by acute toxicity test with drug.
The daily recipe quantity 51g crude drug of 3.3 emmenagogues, twice a day；Emmenagogue raw material powder is relative to the yield of raw material
8.7%, the dysmenorrhea obtained according to that above embodiment of the present invention contains 37% raw material with drug, i.e., granule clinic is quasi- is with dosage
Weight presses 60kg per capita by 12g/ people/day (suitable emmenagogue raw material powder 4.44g/ people/day), i.e. raw material powder 0.074g/kg(women
Meter), which just corresponds to the 1/540 of acute toxicity test in mice maximum dosage-feeding 40g/kg, which just corresponds to rat
The 1/405 of acute toxicity test maximum dosage-feeding 30g/kg, so clinical odd-numbered day drug safety range is larger.
Three, pharmacodynamics test
1, the influence that the dysmenorrhea drug obtained according to that above embodiment of the present invention reacts mouse acetic acid twisting
1.1 methods: by 80 female 28g to 30g mouse points 6 groups: control group (14), C16H25NO2 piece 15mg/kg group (13
Only), emmenagogue 3.8675g crude drug/kg group (13), emmenagogue 7.735g crude drug/kg group (13), emmenagogue 15.47g are raw
Medicine/kg group (13), emmenagogue 30.94g crude drug/kg group (14), it is raw by C16H25NO2 piece 15mg/kg, emmenagogue 3.8675g
Medicine/kg, emmenagogue 7.735g crude drug/kg, emmenagogue 15.47g crude drug/kg, emmenagogue 30.94g crude drug/kg dosage are to mouse
The dysmenorrhea drug obtained according to that above embodiment of the present invention, stomach-filling volume 20ml/kg are given in stomach-filling, and control group gives isometric(al)
Distilled water, the acetum 0.27ml/ of the 0.6% of above-mentioned preparation is injected intraperitoneally only in each mouse of 1h respectively after the last administration, observation
From injecting acetic acid in 15min each mouse writhing number, measurement result is shown in Table 7.
As a result the dysmenorrhea drug obtained according to that above embodiment of the present invention has obviously the writhing response as caused by acetic acid
Inhibiting effect, and dose gradient selects 3.8675g crude drug/kg, 7.735g crude drug/kg, 15.47g crude drug/kg preferable, is shown in Table 7.
C16H25NO2, English name: Tramadol(INN), it is a kind of non-opium central analgesics, is mainly used as easing pain
Medicine can be relieved and commonly arrive serious pain.The medicine be it is artificial synthesized, act on μ-opioid recdptor and norepinephrine
It unites with serum tension prime system.C16H25NO2 can be used for treating medium to serious pain.C16H25NO2 mainly acts on central nervous system
System, over administration can generate dependence, to the effect of human body similar to morphine and heroin.2008, China made tramadol hydrochloride
Control is carried out for psychotropic substances.Therefore, although C16H25NO2 compares the dysmenorrhea drug town obtained according to that above embodiment of the present invention
Pain effect is good, but its side effect is larger, also, can be seen that according to the data of table 1 and obtain according to that above embodiment of the present invention
Dysmenorrhea drug also there is certain analgesic effect.
2, influence of the dysmenorrhea drug obtained according to that above embodiment of the present invention to dysmenorrhea model in mice
Influence of the 2.1 dysmenorrhea drugs obtained according to that above embodiment of the present invention to oxytocins induced mice dysmenorrhea model
Method: 60 mouse (female) are divided equally 5 groups: model group, TONGJINGBAO KELI 3.0g/kg group, emmenagogue 3.8675g
Crude drug/kg group, emmenagogue 7.735g crude drug/kg group, emmenagogue 15.47g crude drug/kg group.Each mouse is only subcutaneously injected by 0.05ml/
Oestradiol benzoate (0.1mg/ is only), starts to be subcutaneously injected oestradiol benzoate 0.025ml/ on the 2nd day only (0.05mg), continuously
9d, from the 7th day, while oestradiol benzoate is subcutaneously injected daily, by TONGJINGBAO KELI 3.0g/kg, emmenagogue 3.8675g
Crude drug/kg, emmenagogue 7.735g crude drug/kg, emmenagogue 15.47g crude drug/kg dosage are given according to the present invention to intragastric administration on mice
The dysmenorrhea drug that above-described embodiment obtains, stomach-filling volume 20ml/kg, control group give the distilled water of isometric(al), continuous 4d,
Oestradiol benzoate (0.1mg/ is only) is only subcutaneously injected by 0.05ml/ for 10th day each mouse, and rear last gives test medicine for 24 hours, gives
60min after medicine, intraperitoneal injection oxytocins 0.2ml/ only (10U/ml), are turned round in mouse writhing incubation period and 15min after record injection
Body number, calculates writhing response inhibiting rate, and record the results are shown in Table 8.
Data processing: indices use (± S) it indicates, using statistic software SPSS (17.0), carry out single factor test between group
Variance analysis (oneway ANOVA).
As a result: the dysmenorrhea drug obtained it can be seen from data in table 8 according to the above embodiment of the present invention is to oxytocins institute
It causes mouse dysmenorrhea writhing response incubation period to there is apparent extension to act on, has apparent reduction effect and effect to writhing response number
It is better than currently used TONGJINGBAO KELI, illustrates, the dysmenorrhea drug obtained according to the above embodiment of the present invention is to treatment dysmenorrhea tool
There is preferable effect.
The 2.2 dysmenorrhea drugs obtained according to that above embodiment of the present invention are to prostaglandin E1Induced mice dysmenorrhea model
Influence
Method: 60 mouse (female) are divided equally 5 groups: model group, TONGJINGBAO KELI 3.0g/kg group, emmenagogue 3.8675g
Crude drug/kg group, emmenagogue 7.735g crude drug/kg group, emmenagogue 15.47g crude drug/kg group.Each mouse is only subcutaneously injected by 0.05ml/
Oestradiol benzoate (0.1mg/ is only), starts to be subcutaneously injected oestradiol benzoate 0.025ml/ on the 2nd day only (0.05mg), continuously
9d, from the 7th day, while oestradiol benzoate is subcutaneously injected daily, by TONGJINGBAO KELI 3.0g/kg, emmenagogue 3.8675g
Crude drug/kg, emmenagogue 7.735g crude drug/kg, emmenagogue 15.47g crude drug/kg dosage are given according to the present invention to intragastric administration on mice
The dysmenorrhea drug that above-described embodiment obtains, stomach-filling volume 20ml/kg, control group give the distilled water of isometric(al), continuous 4d,
Oestradiol benzoate (0.1mg/ is only) is only subcutaneously injected by 0.05ml/ for 10th day each mouse, and rear last gives test medicine for 24 hours, gives
60min after medicine, intraperitoneal injection alprostadil injection 0.2ml/ only (2ml:10 μ g), record mouse writhing incubation period after injection
And writhing number in 15min, writhing response inhibiting rate is calculated, measurement result is shown in Table 9.
Data processing: indices use (± S) it indicates, using statistic software SPSS (17.0), carry out single factor test between group
Variance analysis (oneway ANOVA).
As a result: as shown in Table 9, emmenagogue is to prostaglandin E1Induced mice dysmenorrhea writhing response incubation period, which has, significantly to be prolonged
Long effect, has apparent reduction effect to writhing response number and effect is better than currently used TONGJINGBAO KELI, illustrate, according to this
The dysmenorrhea drug that invention above-described embodiment obtains has preferable effect to treatment dysmenorrhea.
3, influence of the dysmenorrhea drug obtained according to that above embodiment of the present invention to rat uterus microcirculation
Grouping: by 6 groups of the female rats of weight 180g to 200g point: Normal group, model group, TONGJINGBAO KELI
2.2g/kg group, emmenagogue 2.8475g crude drug/kg group, emmenagogue 5.695g crude drug/kg group, emmenagogue 11.39g crude drug/kg group.
It is raw by TONGJINGBAO KELI 2.2g/kg, emmenagogue 2.8475g crude drug/kg, emmenagogue 5.695g crude drug/kg group, emmenagogue 11.39g
Medicine/kg dosage gives the dysmenorrhea drug obtained according to that above embodiment of the present invention, stomach-filling volume 10ml/ to rat oral gavage
Kg, control group give the distilled water of isometric(al), one time a day, are administered 7 days altogether, the 3rd day after gastric infusion starts the same of stomach-filling
When, intramuscular injection stilbestrol 1mg/kg(makes it uniformly enter estrus), one time a day, co-injection 4 times.
Microcirculation detection: after the last administration, by rat, with 3% yellow Jackets anesthetized rat, (45mg/kg, abdominal cavity are infused
Penetrate 1.5ml/kg), back position is fixed, and left uterine angle is gently pulled out abdominal cavity, is placed in and is full of by row abdomen median line notch 2-3cm
On the convex observation platform in the organic glass constant temperature water bath center of 37 DEG C of HW-2000 microcirculation number thermostats of Krebs liquid,
It is irradiated using Multi Position (Fiber Optic llluminator System) cold light source, is schemed using BI-2O00
Picture analysis system dynamic Microcirculation observation assay surface acquisition microscope under video image, select ring-type parteriole branch as
Object is observed, about 30min is stablized, 1 hour after gastric infusion, records the arteriole caliber of selection area, capillary network is handed over
Point counting, blood flow state score value, Blood cell accumulation state score value etc. are used as observation index.
Modeling method: sublingual vein injects oxytocin 2U/ only (0.2ml/ only), then 1min after record injection oxytocin,
3min, 5min, 10min, 15min, 20min These parameters.
Blood flow state is divided into 4 grades:
1. line stream: blood flow is fast, in smooth rope strip, no granular sensation --- and 0 grade；
2. line grain stream: blood flow is very fast, in streak, there is apparent granular sensation --- 1 grade；
3. grain stream: blood flow is slow, at silt shape, flows slow or swing --- 2 grades；
4. stagnating: blood flow cessation is motionless or blood flow is invisible --- 3 grades
Erythrocyte aggregation state is divided into 4 grades:
5. without aggregation --- 0 grade；
6. slight aggregation --- 1 grade；
7. moderate is assembled --- 2 grades；
8. moderate is assembled --- 3 grades
Data statistics
Arteriole caliber, capillary network number of hits are administered front and back percentage change using calculating and indicate, such as after administration
Certain moment microvascular diameter percentage change=[diameter before (certain moment diameter after diameter-modeling before modeling) ÷ modeling] × 100%,
Then t is examined carrying out group to percentage change again；T directly carrying out group to blood flow state score value, Blood cell accumulation state score value
It examines.
It the results are shown in Table 10, table 11, table 12, table 13.
Seen in table 10, emmenagogue 2.8475g crude drug/kg, emmenagogue 5.695g crude drug/kg, emmenagogue 11.39g crude drug/
Kg improves significantly the reduction of the uterus microvascular diameter as caused by oxytocin, and effect is better than TONGJINGBAO KELI
Group.
Seen by table 11, emmenagogue 2.8475g crude drug/kg, emmenagogue 5.695g crude drug/kg, emmenagogue 11.39g crude drug/kg
There is apparent inhibiting effect the reduction of the uterus capillary network number of hits as caused by oxytocin, and effect is better than dysmenorrhea precious
Grain group.
Seen by table 12, emmenagogue 2.8475g crude drug/kg, emmenagogue 5.695g crude drug/kg, emmenagogue 11.39g crude drug/kg
It improves significantly the uterus microvascular blood flow state as caused by oxytocin, and effect is better than TONGJINGBAO KELI group.
Seen by table 13, emmenagogue 2.8475g crude drug/kg, emmenagogue 5.695g crude drug/kg, emmenagogue 11.39g crude drug/kg
It improves significantly the uterus capilary Blood cell accumulation state as caused by oxytocin, and effect is better than TONGJINGBAO KELI
Group.
It is followed in conclusion the dysmenorrhea drug obtained according to that above embodiment of the present invention is micro- to the uterus as caused by oxytocin
Ring obstacle index of correlation improves significantly.
In summary it is found that the Kazak medicine obtained according to that above embodiment of the present invention: dysmenorrhea drug utilization Kazak
Medicinal material be made, preparation method is simple, and drug used is all warm nature drug, with channel warming and blood nourishing, menstruction regulating and pain relieving, nontoxic pair work
With using the dysmenorrhea drug obtained according to that above embodiment of the present invention, when treating dysmenorrhea, curative effect is short, and it is bright to improve dysmenorrhea symptom
Aobvious, treated effect is high, has no toxic side effect, and no drug resistance phenomenon is uneasy to recur after treatment, can effectively release for patient ailing.
Claims (8)
1. a kind of dysmenorrhea drug, it is characterised in that raw material according to parts by weight: 3 parts to 10 parts of corydalis tuber, 6 parts to 12 parts of Radix Angelicae Sinensis,
3 parts to 10 parts of Rhizoma Chuanxiong, 3 parts to 9 parts of folium artemisiae argyi, 6 parts to 10 parts of the root of three-nerved spicebush, 3 parts to 6 parts and 0.5 part to 1 of root of Altai Daphne of fennel seeds
Part；The dysmenorrhea is obtained as follows with drug: the first step, by the desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and
Root of Altai Daphne mixes to form mixing medicinal material, and it is laggard that water of the mixing quality of medicinal material 6 again to 9 times is added into mixing medicinal material
Row steam distillation, the temperature of steam distillation is 100 DEG C to 200 DEG C, the time is 4 hours to 6 hours, is divided after steam distillation
The volatile oil of aqueous solution and extraction is not obtained；Second step carries out the desired amount of corydalis tuber refluxing extraction 1 time to 3 times, flows back every time
Extracting the addition corydalis tuber quality 4 into corydalis tuber is again 30% to 95% ethanol water to 10 times of volumetric concentration, and reflux mentions every time
The temperature taken is 60 DEG C to 100 DEG C, the time is 0.5 hour to 3.5 hours, and filtrate is obtained by filtration after each refluxing extraction, is merged
Mixed filtrate is obtained after filtrate, recycles the ethyl alcohol in mixed filtrate, mixed filtrate and first step refluxing extraction after recycling ethyl alcohol
Obtained aqueous solution merges to obtain mixed solution, is 1% by mass water content is obtained after mixed solution concentration, vacuum drying
To 10% dry extract, dry extract is broken into 100 mesh to 200 targeted fine powders；1 times of fine powder quality is added into fine powder for third step
Sweetener to 3 times of dextrin, fine powder quality 0.1% to 5% obtains mixing fine powders, is added and waves into the volatile oil that the first step obtains
Hair oil quality 0.5 is diluted to obtain dilution volatile oil again to the ethanol water that 2 times of volumetric concentration is 80% to 95%, will mix
It closes fine powder and particle is made in dilution volatile oil after mixing, obtain dysmenorrhea drug after dry, whole grain.
2. dysmenorrhea drug according to claim 1, it is characterised in that the number of refluxing extraction is 2 times in second step；Or/
With, in second step, recycle mixed filtrate in ethyl alcohol ethyl alcohol into mixed filtrate mass content less than 10%.
3. dysmenorrhea drug according to claim 1 or 2, it is characterised in that concentration is is concentrated under reduced pressure in second step, decompression
The temperature of concentration is 50 DEG C to 80 DEG C, pressure is -0.05MPa to -0.09MPa；Or/and vacuum drying temperature in second step
It is -0.05MPa to -0.09MPa for 50 DEG C to 80 DEG C, pressure.
4. dysmenorrhea drug according to claim 3, it is characterised in that in third step, by mixing fine powders and dilution volatile oil
Be constructed to after mixing by 10 meshes, cannot be by the particle of 80 meshes；Or/and in third step, by particle drying to
Grain mass water content is 0.5% to 5%；Or/and the particle in third step after drying is can pass through 10 meshes, cannot pass through 80 mesh
Sieve carries out whole grain.
5. a kind of preparation method of dysmenorrhea drug according to claim 1, it is characterised in that carry out in the steps below: the
One step mixes the desired amount of Radix Angelicae Sinensis, Rhizoma Chuanxiong, folium artemisiae argyi, the root of three-nerved spicebush, fennel seeds and root of Altai Daphne to form mixing medicinal material,
Mixing quality of medicinal material 6 is added into mixing medicinal material again to steam distillation is carried out after 9 times of water, the temperature of steam distillation is
100 DEG C to 200 DEG C, the time be 4 hours to 6 hours, the volatile oil of aqueous solution and extraction is respectively obtained after steam distillation；Second
Step carries out the desired amount of corydalis tuber refluxing extraction 1 time to 3 times, and corydalis tuber quality 4 is added into corydalis tuber again to 10 for each refluxing extraction
Times volumetric concentration be 30% to 95% ethanol water, the temperature of each refluxing extraction is 60 DEG C to 100 DEG C, the time 0.5
Hour to 3.5 hours, filtrate is obtained by filtration after each refluxing extraction, mixed filtrate is obtained after merging filtrate, recycles mixed filtrate
In ethyl alcohol, recycle ethyl alcohol after mixed filtrate merge to obtain with the aqueous solution that first step refluxing extraction obtains mix it is molten
Liquid will obtain the dry extract that mass water content is 1% to 10% after mixed solution concentration, vacuum drying, dry extract is broken into 100
Mesh is to 200 targeted fine powders；Dextrin of the fine powder quality 1 again to 3 times, fine powder quality 0.1% to 5% are added into fine powder for third step
Sweetener obtains mixing fine powders, and volatilization oil quality 0.5 is added into the volatile oil that the first step obtains and is to 2 times of volumetric concentration again
80% to 95% ethanol water is diluted to obtain dilution volatile oil, and mixing fine powders and dilution volatile oil are made after mixing
At particle, dysmenorrhea drug is obtained after dry, whole grain.
6. the preparation method of dysmenorrhea drug according to claim 5, it is characterised in that time of refluxing extraction in second step
Number is 2 times；Or/and in second step, the mass content of ethyl alcohol ethyl alcohol into mixed filtrate in mixed filtrate is recycled less than 10%.
7. the preparation method of dysmenorrhea drug according to claim 5 or 6, it is characterised in that concentration is decompression in second step
Concentration, the temperature of reduced pressure is 50 DEG C to 80 DEG C, pressure is -0.05MPa to -0.09MPa；Or/and vacuum is dry in second step
Dry temperature is 50 DEG C to 80 DEG C, pressure is -0.05MPa to -0.09MPa.
8. the preparation method of dysmenorrhea drug according to claim 7, it is characterised in that in third step, by mixing fine powders and
Dilution volatile oil be constructed to after mixing by 10 meshes, cannot be by the particle of 80 meshes；Or/and in third step, general
Grain drying to granular mass water content is 0.5% to 5%；Or/and in third step it is dry after particle 10 meshes, no can be passed through
Whole grain can be carried out by 80 meshes.
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| CN104688941A (en) * | 2015-03-17 | 2015-06-10 | 苏州市天灵中药饮片有限公司 | Traditional Chinese medicine decoction for relieving dysmenorrhea and preparation method thereof |
| CN107335046A (en) * | 2017-08-29 | 2017-11-10 | 南京多宝生物科技有限公司 | A kind of medicine moxa roll for treating dysmenorrhoea and preparation method thereof |
| CN117695371B (en) * | 2024-02-06 | 2024-04-19 | 中科创导(山东)生物科技有限公司 | Traditional Chinese medicine fermentation preparation for treating dysmenorrhea and preparation method thereof |
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| CN103239702A (en) * | 2013-05-24 | 2013-08-14 | 温美珍 | Medicament for treating dysmenorrhea |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103239702A (en) * | 2013-05-24 | 2013-08-14 | 温美珍 | Medicament for treating dysmenorrhea |
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