CN104323976A - Preparation method of composition with skin tightening effect - Google Patents

Abstract

The invention relates to a preparation method of a composition with skin tightening effect. The preparation method comprises the following steps of A: weighing a klamath blue-green algae extractive, a chlamydomonas nivalis extractive, ethanol, a wild soybean seed extractive, a methyl methacrylate/ethylene glycol dmethylacrglate cross-linked polymer or a allyl methacrylate cross-linked polymer, uniformly mixing, and then heating to obtain a-phase liquid; B: weighing a cell enabling compound, an undaria pinnatifida extractive, trehalose and deionized water, and uniformly stirring to obtain b-phase liquid; C: mixing the a-phase liquid and the b-phase liquid to obtain a mixed solution c; D: weighing a padina pavonica thallus extractive and the mixed solution c, uniformly mixing and stirring to obtain a mixed solution d; and E: loading the mixed solution d into a freeze-drying glass bottle, carrying out freeze-drying to obtain freeze-dried powder, sealing in a vacuum environment to obtain the freeze-dried powder of the composition with the skin tightening effect. The composition prepared through the method disclosed by the invention has the advantages of obvious effect, good coexistence among active matters, stable quality and long retention period.

Description

A kind of preparation method with the compositions of skin-tightening effect

Technical field

The present invention relates to technical field of cosmetic preparation, particularly a kind of preparation method with the compositions of skin-tightening effect.

Background technology

With advancing age, skin there will be wrinkle, the evidence for senescence such as lax.Skin aging is the same with whole body aging, is the process caused by many factors combined effect.Need for the various factor causing skin aging, corresponding effective ingredient is combined, just can reach and effectively resist aging comparatively comprehensively, the effect of firm skin.

Existing activity of fighting against senium material is all higher to environmental requirements such as the compatibilitys of temperature, illumination, air, solution, and single activity of fighting against senium thing can only work for certain one deck of skin, such as powder ball fan algae (PADINA PAVONICA) thallus extract, main component is biological calcium, the synthesis of the calcareous structure of epidermis cell can be increased, strengthen resistance and the cohesiveness of epidermis, improve skin water storage power and water conservation degree, repair skin barrier, site of action is the skin corium that epidermis but cannot arrive skin.Carat Maas blue-green alge (KLAMATH LAKE ALGAE) extract, main component is several mineral materials, protein, unsaturated fatty acid etc., supplement mineral and unsaturated lipid constituents, promote the synthesis of integrin, site of action is epidermis dermis junction (DEJ); Thallus Laminariae (UNDARIA PINNATIFIDA) extract, main component is fucoidin, and it can activate the synthesis of synthesizing with elastin laminin, collagen protein, hyaluronic acid, and site of action is the skin corium being confined to skin.If mixed by various active thing, then can there is the problem of the compatibility, oil-soluble active thing cannot well mix with water-soluble active thing, and mixed liquor there will be the problems such as variable color, precipitation, layering, and quality is unstable, and the storage life is short.Such as Thallus Laminariae extract and powder ball are fanned after algae thallus extract solution mixes, the organic calcium in algae thallus extract fanned by fucoidin in Thallus Laminariae extract and powder ball, can slowly condense, usually there will be precipitation once all left and right time, cause active matter to lose efficacy effect, therefore can not mix existence for a long time in aqueous.

Summary of the invention

The technical problem to be solved in the present invention is, a kind of preparation method with the compositions of skin-tightening effect is provided, the method overcome that existing anti-aging cosmetics efficacy effect approach is single, multiple efficacies active matter can not coexist, the technical problem that skin anti-aging effect is bad, product stability is poor, the pot-life is short.

For solving the problems of the technologies described above, the present invention by the following technical solutions: a kind of preparation method with the compositions of skin-tightening effect, is characterized in that: adopt following steps:

A: 5 ~ 10:1 in mass ratio ~ 3:3 ~ 5:1 ~ 3:3 ~ 5 take a carat Maas blue-green alge extract, snowfield chlamydomonas extract, ethanol, Wild soybean seed extract, methyl methacrylate/glycol methacrylate cross linked polymer or allyl methacrylate class cross linked polymer, by above composition mix homogeneously post-heating to 70 ~ 75 DEG C, obtain a phase liquid; The particle size range of described methyl methacrylate/glycol methacrylate cross linked polymer is 5-12 μm, and oil factor is 180 ± 20; The particle size range of described allyl methacrylate class cross linked polymer is 5-12 μm, and oil factor is 180 ± 20;

B: 3 ~ 5:1 in mass ratio ~ 3:5 ~ 10:30 ~ 50 take cell and to energize complex, Thallus Laminariae extract, trehalose and deionized water, and be heated to 70-75 DEG C, stir to obtain b phase liquid; Described cell energize complex be adenosine, creatine, L-carnitine, GP4G in mass ratio 2:2:2:1 composition pulverulent mixture;

C: 1:3 ~ 5 in mass ratio, by steps A gained a phase liquid and step B gained b phase liquid mixing, at 70 ~ 75 DEG C, stir 15-20 minute with agitator with the rotating speed of 2000rpm, cool to room temperature, obtains mixed liquor c;

D: the ratio of 1:50 ~ 70 in mass ratio, takes powder ball fan algae thallus extract and mixed liquor c, powder ball is fanned algae thallus extract and add mixed liquor c, stir, obtain mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and be placed in ultra cold storage freezer, the temperature arranging ultra cold storage freezer is-40 DEG C, and pre-freeze 2 is little of fully charge;

F: start freezer dryer, after the temperature in freezer dryer drops to-85 DEG C, lyophilizing vial is put into freezer dryer, open vacuum system, pressure in freezer dryer is reduced to below 15pa, and after pressure stability, dry 48h, obtains lyophilized powder, in vacuum environment, lyophilizing draw glass bottle pressure cover is sealed, obtain a kind of lyophilized powder with the compositions of skin-tightening effect.

The active matter that the present invention can act on skin different structure level respectively combines, and then utilizes freeze-dry process to process, is prepared into lyophilized powder, overcomes powder mixture mixing in prior art uneven, the technical problem that dissolution velocity is slow.Lyophilized powder prepared by the present invention is packed under vacuum conditions, and form the vial fractional pack of single, inject pure water during use in vial, the lyophilized powder in vial is dissolved into emulsion instantaneously, and then just can be applied on skin, skinfeel is comfortable.

The particle size range of described methyl methacrylate/glycol methacrylate cross linked polymer is 5-12 μm, and oil factor is 180 ± 20.This methyl methacrylate/glycol methacrylate cross linked polymer is neither too hard, nor too soft, skinfeel is comfortable.

The particle size range of described allyl methacrylate class cross linked polymer is 5-12 μm, and oil factor is 180 ± 20.This allyl methacrylate class cross linked polymer is neither too hard, nor too soft, skinfeel is comfortable.

The cell that the present invention selects is energized (CELL ENERGIZER) complex, for adenosine (ADENOSINE): creatine (CREATINE): L-carnitine (L-CARNITINE), the pulverulent mixture of GP4G (DIGUANOSINE TETRAPHOPHATE) 2:2:2:1 in mass ratio, the energy that this complex can provide cell to carry out required for physiological activity, skin is helped effectively to resist the light radiation causing senilism, comprise infrared ray, and ultraviolet radiation, and the ambient temperature pressure of senilism may be caused, as overheated and mistake cold environment, slow down skin because suffering outer ultraviolet, infrared, the aging that overheated and supercool factor causes.Powder ball fan algae (PADINA PAVONICA) thallus extract, main component is biological calcium, and it can increase the synthesis of the calcareous structure of epidermis cell, strengthen resistance and the cohesiveness of epidermis, improve skin water storage power and water conservation degree, repair skin barrier, site of action is the epidermal area of skin; Carat Maas blue-green alge (KLAMATH LAKE ALGAE) extract, main component is several mineral materials, protein, unsaturated fatty acid etc., supplement mineral and unsaturated lipid constituents, promote the synthesis of integrin, site of action is epidermis dermis junction (DEJ); Snowfield chlamydomonas (CHLAMYDOMONAS NIVALIS) extract, main component is carotenoid, and it can suppress the activity of metalloproteases, and has the effect of protection DNA telomere, and site of action is the skin corium of skin.Thallus Laminariae (UNDARIA PINNATIFIDA) extract, main component is fucoidin, its can activate synthesize to elastin laminin, collagen protein, hyaluronic acid, ripe, assemble and link 14 relevant genes, promote collagen protein, elastin laminin, hyaluronic synthesis in extracellular matrix, site of action is the skin corium of skin.

The present invention is according to the mechanism for skin aging, energized by cell complex, snowfield chlamydomonas extract, Thallus Laminariae extract, powder ball fan algae thallus extract, carat Maas blue-green alge is extracted and combines, go deep into each Rotating fields of skin, from epidermal area, epidermis dermis junction, skin corium, skin is comprehensively repaired, opposing skin ageing problem, experimental data proves that said composition significantly can increase skin elasticity and reduce wrinkle, reaches the effect of the skin that compacts.

But all there is the defect in performance in above-mentioned several activity of fighting against senium thing, as: snowfield chlamydomonas extract, carat Maas blue-green alge extract contain carotenoid and unsaturated fatty acid, if do not add protection in atmosphere, very easily oxidized, there is rancidity, lose effectiveness; And the two is all oil-soluble ingredients.Cell is energized complex, Thallus Laminariae extract, powder ball fan algae thallus extract, these three kinds of compositions are water soluble ingredients, the organic calcium in algae thallus extract fanned by fucoidin in Thallus Laminariae extract and powder ball, can slowly condense, usually there will be precipitation once all left and right time, therefore can not mix for a long time in aqueous and deposit.In five kinds of active component, wherein two kinds of taste oil-soluble ingredients, are three kinds of water soluble ingredients in addition, simply mix and there will be lamination, can not form uniform mixture.

The present invention adopts a kind of in methyl methacrylate/glycol methacrylate cross linked polymer, allyl methacrylate class cross linked polymer or any both it to arrange in pairs or groups than mixture and ethanol, Wild soybean seed extract; as carrier material; add appropriate trehalose as freeze drying protectant, preparation has the lyophilized powder of effect of compacting simultaneously.The present invention is by selecting suitable carrier material, suitable product preparation and freeze-dry process, oil-soluble ingredients and water soluble ingredient mix homogeneously can be made, also avoid the coagulative precipitation phenomenon when Thallus Laminariae extract that there will be in storage process and the thallus extract mixing of powder ball fan algae simultaneously.Namely block after lyophilizing is glanded encapsulation in vacuum freeze-drier, therefore there is not the oxidized problem of active matter yet, and the block formed after lyophilizing is loose porous, as long as add pure water when use, lyophilized powder can dissolve instantaneously becomes emulsion, disposablely to be finished, emulsion need not store, by the lyophilized powder with crease-resistant effect prepared by method provided by the invention, medicine stability by standard is tested, stability test is carried out to the lyophilized powder being glanded encapsulation in vacuum freeze-drier, determines that its shelf-life can reach 4 years.

prepare the test of sample senile-resistant efficacy:

Volunteer: 30 people (30 to 65 years old ages)

Research: double-blind

Using method: lyophilizing sample A, B, C, D, E, F, G, in lyophilizing sample: the quality of pure water=1:9, than ratio, adds pure water, shake up rear formation emulsion, then smear use;

Application: every day uses twice, and each half face uses 2 mg/cm2 emulsions, and emulsion is all newly configure by above method at every turn

Cycle: 28 days

Determining instrument: German CK MPA580 elasticity tester, U.S. CANFIELD company VISIA skin analysis tester

Sooner or later get appropriate above emulsion uniform application after cleaning face every day in left face, the right face smears not containing the emulsion of the blank lyophilizing carrier of active component.

Use weekly MPA580 elasticity tester, everyone experimental data of VISIA skin analysis tester record.Experimental data is as following table:

Sample ID	Left side appearance is to right side skin elasticity relative increase (%)	Left side appearance is to the relative reduction of the right side wrinkle of skin degree of depth (%)
			A	27.6	28.2
B	17.3	16.5
			C	16.8	18.4
D	18.5	18.2
			E	19.6	20.1
F	16.5	18.5
			G	5.5	5.2

Sample A: the sample prepared by embodiment 3 same process;

Sample B: the sample prepared by embodiment 3 same process, but do not add cell and to energize complex;

Sample C: the sample prepared by embodiment 3 same process, but do not add snowfield chlamydomonas extract;

Sample D: the sample prepared by embodiment 3 same process, but do not add a carat Maas blue-green alge extract;

Sample E: the sample prepared by embodiment 3 same process, but do not add Thallus Laminariae extract;

Sample F: the sample prepared by embodiment 3 same process, but do not add powder ball fan algae thallus extract;

Sample G: the sample prepared by embodiment 3 same process, but any one of not adding that cell energizes in complex, snowfield chlamydomonas extract, Thallus Laminariae extract, powder ball fan algae thallus extract, carat Maas blue-green alge extract five kinds of active matters;

As can be seen from the above table, sample A, B, C, D, E, F and G compare, there is obvious tightening effect, prove that energize complex, snowfield chlamydomonas extract, Thallus Laminariae extract, powder ball fan algae thallus extract and carat Maas blue-green alge extract of cell compacts to skin and all have certain actual effect.But after using sample A surrounding, the improvement situation of skin elasticity and wrinkle depth is all better than sample B, C, D, E, F and G, shows the tightening effect of obvious Synergistic.

In sum, the compositions of skin-tightening effect that prepared by the present invention have, effect is remarkable, compossibility between active matter is good, steady quality, storage life limit for length, easy to use, overcome that existing anti-aging cosmetics efficacy effect approach is single, multiple efficacies active matter can not coexist, the technical problem that skin anti-aging effect is bad, product stability is poor, the pot-life is short.

Detailed description of the invention

embodiment 1

A kind of preparation method with the compositions of skin-tightening effect:

A: 5:1:3:1:3 takes 38.46g carat Maas blue-green alge extract, 7.69g snowfield chlamydomonas extract, 23.08g analytical pure ethanol, 7.69g Wild soybean seed extract, 23.08g methyl methacrylate/glycol methacrylate cross linked polymer in mass ratio, by above composition mix homogeneously post-heating to 70 DEG C, obtain a phase; Described methyl methacrylate/glycol methacrylate cross linked polymer adopts the MICROSPONGE of AMCOL company of the U.S., and its particle size range is 5-12 μm, and oil factor is 180 ± 20;

B: 3:1:5:30 takes 23.07g cell and to energize complex, 7.69g Thallus Laminariae extract, 38.46g trehalose and 230.7g deionized water in mass ratio, and be heated to 70 DEG C, stir to obtain b phase;

C: 1:3 weighs in mass ratio, 100g a phase liquid is added 300g b phase liquid, at 70 DEG C, stirs 15 minutes, cool to room temperature, obtain mixed liquor c with Japanese PRIMIX agitator with the rotating speed of 2000rpm;

D: 1:50 takes 8g powder ball fan algae thallus extract and adds 400g mixed liquor c in mass ratio, stirs, obtains mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and-40 DEG C of pre-freezes 2 are little of fully charge in ultra cold storage freezer;

F: the compressor starting freezer dryer, when freeze dryer temperature drops to-85 DEG C, the sample of fully charge is put in freeze dryer, open vacuum system, pressure in freeze dryer is reduced to below 15pa, and dry 48h after pressure stability, obtains a kind of lyophilized powder, by lyophilizing draw glass bottle pressure cover in vacuum environment, obtain a kind of composition freeze-dried powder with skin-tightening effect.

embodiment 2

A kind of preparation method with the compositions of skin-tightening effect:

A: 8:2:4:2:4 takes 40g carat Maas blue-green alge extract, 10g snowfield chlamydomonas extract, 20g analytical pure ethanol, 10g Wild soybean seed extract, 20g allyl methacrylate class cross linked polymer in mass ratio, by above composition mix homogeneously post-heating to 75 DEG C, obtain a phase; Described allyl methacrylate class cross linked polymer selects the POLYTRAP 7603 of AMCOL company of the U.S., and its particle size range is 5-12 μm, and oil factor is 180 ± 20;

B: 4:2:8:40 takes 29.63g cell and to energize complex, 14.81g Thallus Laminariae extract, 59.26.46g trehalose and 296.3g deionized water in mass ratio, and be heated to 70 DEG C, stir to obtain b phase;

C: 1:4 weighs in mass ratio, 100g a phase liquid is added 400g b phase liquid, at 70 DEG C, stirs 15 minutes, cool to room temperature, obtain mixed liquor c with Japanese PRIMIX agitator with the rotating speed of 2000rpm;

D: 1:55 takes 9.09g powder ball fan algae thallus extract and adds 500g mixed liquor c in mass ratio, stirs, obtains mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and-40 DEG C of pre-freezes 2 are little of fully charge in ultra cold storage freezer;

F: the compressor starting freezer dryer, when freeze dryer temperature drops to-85 DEG C, the sample of fully charge is put in freeze dryer, open vacuum system, pressure in freeze dryer is reduced to below 15pa, and dry 48h after pressure stability, obtains a kind of lyophilized powder, by lyophilizing draw glass bottle pressure cover in vacuum environment, obtain a kind of composition freeze-dried powder with skin-tightening effect.

 

embodiment 3

A kind of preparation method with the compositions of skin-tightening effect:

A: 10:3:4:3:5 takes the mixture of 40g carat Maas blue-green alge extract, 12g snowfield chlamydomonas extract, 16g analytical pure ethanol, 12g Wild soybean seed extract, 20g methyl methacrylate/glycol methacrylate cross linked polymer and allyl methacrylate class cross linked polymer in mass ratio, by above composition mix homogeneously post-heating to 75 DEG C, obtain a phase; The MICROSPONGE of AMCOL company of described methyl methacrylate/glycol methacrylate cross linked polymer preferred U.S., its particle size range is 5-12 μm, and oil factor is 180 ± 20; The POLYTRAP 7603 of AMCOL company of the described allyl methacrylate class cross linked polymer preferred U.S., its particle size range is 5-12 μm, and oil factor is 180 ± 20;

B: 5:3:10:50 takes 36.76g cell and to energize complex, 22.06g Thallus Laminariae extract, 73.53g trehalose and 367.6g deionized water in mass ratio, and be heated to 70 DEG C, stir to obtain b phase;

C: 1:5 weighs in mass ratio, 100g a phase liquid is added 500g b phase liquid, at 70 DEG C, stirs 20 minutes, cool to room temperature, obtain mixed liquor c with Japanese PRIMIX agitator with the rotating speed of 2000rpm;

D: 1:60 takes 10g powder ball fan algae thallus extract and adds 600g mixed liquor c in mass ratio, stirs, obtains mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and-40 DEG C of pre-freezes 2 are little of fully charge in ultra cold storage freezer;

F: the compressor starting freezer dryer, when freeze dryer temperature drops to-85 DEG C, the sample of fully charge is put in freeze dryer, open vacuum system, pressure in freeze dryer is reduced to below 15pa, and dry 48h after pressure stability, obtains a kind of lyophilized powder, by lyophilizing draw glass bottle pressure cover in vacuum environment, a kind of composition freeze-dried powder of skin-tightening effect must be had.

 

embodiment 4

A kind of preparation method with the compositions of skin-tightening effect:

A: 8:2:5:2:4 takes the mixture of 38.09g carat Maas blue-green alge extract, 9.52g snowfield chlamydomonas extract, 23.83g analytical pure ethanol, 9.52g Wild soybean seed extract, 19.05g methyl methacrylate/glycol methacrylate cross linked polymer and allyl methacrylate class cross linked polymer in mass ratio, by above composition mix homogeneously post-heating to 75 DEG C, obtain a phase; The MICROSPONGE of AMCOL company of described methyl methacrylate/glycol methacrylate cross linked polymer preferred U.S., its particle size range is 5-12 μm, and oil factor is 180 ± 20; The POLYTRAP 7603 of AMCOL company of the described allyl methacrylate class cross linked polymer preferred U.S., its particle size range is 5-12 μm, and oil factor is 180 ± 20;

B: 5:3:10:50 takes 34.78g cell and to energize complex, 17.39g Thallus Laminariae extract, 86.96g trehalose and 260.87.6g deionized water in mass ratio, and be heated to 70 DEG C, stir to obtain b phase;

C: 1:4 weighs in mass ratio, 100g a phase liquid is added 400g b phase liquid, at 70 DEG C, stirs 20 minutes, cool to room temperature, obtain mixed liquor c with Japanese PRIMIX agitator with the rotating speed of 2000rpm;

D: 1:65 takes 7.69g powder ball fan algae thallus extract and adds 500g mixed liquor c in mass ratio, stirs, obtains mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and-40 DEG C of pre-freezes 2 are little of fully charge in ultra cold storage freezer;

F: the compressor starting freezer dryer, when freeze dryer temperature drops to-85 DEG C, the sample of fully charge is put in freeze dryer, open vacuum system, pressure in freeze dryer is reduced to below 15pa, and dry 48h after pressure stability, obtains a kind of lyophilized powder, by lyophilizing draw glass bottle pressure cover in vacuum environment, obtain a kind of composition freeze-dried powder with skin-tightening effect.

embodiment 5

A kind of preparation method with the compositions of skin-tightening effect:

A: 5:1:5:1:3 takes the mixture of 33.33g carat Maas blue-green alge extract, 6.67g snowfield chlamydomonas extract, 33.33g analytical pure ethanol, 6.67g Wild soybean seed extract, 20g methyl methacrylate/glycol methacrylate cross linked polymer and allyl methacrylate class cross linked polymer in mass ratio, by above composition mix homogeneously post-heating to 75 DEG C, obtain a phase; The MICROSPONGE of AMCOL company of described methyl methacrylate/glycol methacrylate cross linked polymer preferred U.S., its particle size range is 5-12 μm, and oil factor is 180 ± 20; The POLYTRAP 7603 of AMCOL company of the described allyl methacrylate class cross linked polymer preferred U.S., its particle size range is 5-12 μm, and oil factor is 180 ± 20;

B: 5:1:5:50 takes 24.59g cell and to energize complex, 4.92g Thallus Laminariae extract, 24.59g trehalose and 245.9g deionized water in mass ratio, and be heated to 75 DEG C, stir to obtain b phase;

C: 1:3 weighs in mass ratio, 100g a phase liquid is added 300g b phase liquid, at 70 DEG C, stirs 15 minutes, cool to room temperature, obtain mixed liquor c with Japanese PRIMIX agitator with the rotating speed of 2000rpm;

D: 1:70 takes 5.71g powder ball fan algae thallus extract and adds 400g mixed liquor c in mass ratio, stirs, obtains mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and-40 DEG C of pre-freezes 2 are little of fully charge in ultra cold storage freezer;

F: the compressor starting freezer dryer, when freeze dryer temperature drops to-85 DEG C, the sample of fully charge is put in freeze dryer, open vacuum system, pressure in freeze dryer is reduced to below 15pa, and dry 48h after pressure stability, obtains a kind of lyophilized powder, by lyophilizing draw glass bottle pressure cover in vacuum environment, obtain a kind of composition freeze-dried powder with skin-tightening effect.

The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.

Claims (1)

1. there is a preparation method for the compositions of skin-tightening effect, it is characterized in that: adopt following steps:

A: 5 ~ 10:1 in mass ratio ~ 3:3 ~ 5:1 ~ 3:3 ~ 5 take a carat Maas blue-green alge extract, snowfield chlamydomonas extract, ethanol, Wild soybean seed extract, methyl methacrylate/glycol methacrylate cross linked polymer or allyl methacrylate class cross linked polymer, by above composition mix homogeneously post-heating to 70 ~ 75 DEG C, obtain a phase liquid; The particle size range of described methyl methacrylate/glycol methacrylate cross linked polymer is 5-12 μm, and oil factor is 180 ± 20; The particle size range of described allyl methacrylate class cross linked polymer is 5-12 μm, and oil factor is 180 ± 20;

B: 3 ~ 5:1 in mass ratio ~ 3:5 ~ 10:30 ~ 50 take cell and to energize complex, Thallus Laminariae extract, trehalose and deionized water, and be heated to 70-75 DEG C, stir to obtain b phase liquid; Described cell energize complex be adenosine, creatine, L-carnitine, GP4G in mass ratio 2:2:2:1 composition pulverulent mixture;

C: 1:3 ~ 5 in mass ratio, by steps A gained a phase liquid and step B gained b phase liquid mixing, at 70 ~ 75 DEG C, stir 15-20 minute with agitator with the rotating speed of 2000rpm, cool to room temperature, obtains mixed liquor c;

D: the ratio of 1:50 ~ 70 in mass ratio, takes powder ball fan algae thallus extract and mixed liquor c, powder ball is fanned algae thallus extract and add mixed liquor c, stir, obtain mixed liquor d;

E: mixed liquor d is loaded lyophilizing vial, and be placed in ultra cold storage freezer, the temperature arranging ultra cold storage freezer is-40 DEG C, and pre-freeze 2 is little of fully charge;

F: start freezer dryer, after the temperature in freezer dryer drops to-85 DEG C, lyophilizing vial is put into freezer dryer, open vacuum system, pressure in freezer dryer is reduced to below 15pa, and after pressure stability, dry 48h, obtains lyophilized powder, in vacuum environment, lyophilizing draw glass bottle pressure cover is sealed, obtain a kind of lyophilized powder with the compositions of skin-tightening effect.