CN104311614A - Method for extracting mangiferin from mango leaves - Google Patents
Method for extracting mangiferin from mango leaves Download PDFInfo
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- CN104311614A CN104311614A CN201410619517.9A CN201410619517A CN104311614A CN 104311614 A CN104311614 A CN 104311614A CN 201410619517 A CN201410619517 A CN 201410619517A CN 104311614 A CN104311614 A CN 104311614A
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- extraction
- mengiferin
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- folium mangiferae
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- Life Sciences & Earth Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
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Abstract
The invention discloses a method for extracting mangiferin from mango leaves. The method includes the following steps: raw material processing, high-pressure extraction, aqueous two-phase extraction, desalination, and recrystallization to obtain the mangiferin. The method provided by the invention has the advantages of high mangiferin content, high extraction rate, simple process, suitability for production, low production cost and the like.
Description
Technical field
The present invention relates to the extracting method of biological technical field, is a kind of method extracting Mengiferin from Folium mangiferae specifically.
Background technology
Mango is aiphyllium, originates in India, and existing tropical various places are all planted, and cultivate in South China, and by Guangxi, Guangdong, village, various places, Hainan or room residence, wetting a little soil is scattered about like the stars plant as seen.Folium mangiferae is the leaf of Anacardiaceae plant mango Mangifera indica L, for Guangxi Local commonly uses Chinese medicine, record in " Guangxi Chinese medicinal materials standard " nineteen ninety version, contained Mengiferin (Mangiferin) is Folium mangiferae cough-relieving, reduce phlegm, the main active ingredient of anti-inflammatory, Mengiferin has anti-inflammatory, antiviral, nervus centralis suppresses and immunosuppressive action, also has anti peroxidation of lipid, antitumor action, conventional to treat hepatitis, Mengiferin treatment chronic bronchitis has good therapeutic effect, Mengiferin has obvious toxic action to hepatoma cell strain, energy liver cancer apoptosis reducing, Mengiferin has anti-oxidant vigor and delaying senility function.
Disclosed Mengiferin extracting and purifying method mostly is water extract-alcohol precipitation, alcohol extracting macroporous resin purification method at present, as Chinese patent CN100475830C " high purity Mengiferin preparation method ", patent CN101759689A " a kind of preparation method of Mengiferin " and patent CN101429222A " extract a kind of method of Mengiferin ", all adopt macroporous resin purification as main separating step, but adopt macroporous resin purification method, resin is with front needing process, purifying need through absorption and elution process, and the production cycle is long.When carrying out purifying wash-out with macroporous resin, due to physical adsorption, also easily causing a part of sample extremely to adsorb, and causing the rate of transform to reduce.Adopt macroporous resin purification effluent volume comparatively large in addition, the solvent recuperation time is long, adds production cost, and solvent recuperation produces more waste liquid, causes certain burden to environmental protection.In addition due to the use of macroporous resin, add the risk introducing the organic residual solvents such as benzene,toluene,xylene, vinylbenzene, alkanes, diethylbenzene class, the security of product is exerted a certain influence.
In sum, study a kind of simple possible, the extracting method of Mengiferin that environmental pollution is little has good realistic meaning.
Summary of the invention
The object of this invention is to provide a kind of method extracting Mengiferin from Folium mangiferae, there is technique simple, be applicable to producing, the advantages such as the time is short.
The present invention is achieved through the following technical solutions, and specifically comprises the steps:
1) Feedstock treating: Folium mangiferae is pulverized or smashes;
2) high pressure extract: by the raw material after process, put into pressure pan, add the pure water of identical weight, be forced into 4kgf/cm
2, make its pressure uniform, and keep 10-15min, recover normal pressure, filter, obtain extracting solution;
3) be separated: aqueous two-phase extraction is carried out to Folium mangiferae, obtain the upper lower phase with extraction mutually of extraction, the double-aqueous phase system that aqueous two-phase extraction uses is made up of polyoxyethylene glycol, sodium hydroxide and water, the mass percent of described polyoxyethylene glycol in described double-aqueous phase system is 10-20%, the mass percent of described sodium hydroxide in described double-aqueous phase system is 10-20%, surplus is water, and the weight-average molecular weight of described polyoxyethylene glycol is 1000-8000;
4) subsequent disposal: by step 3) phase in the extraction that obtains, desalination, recrystallization, by step 3) phase under the extraction that obtains, add and be equivalent to extract lower phase mass percent 5-10% sodium hydroxide, desalination, recrystallization;
5) the acquisition thing after merging recrystallization obtains Mengiferin.
Folium mangiferae of the present invention is fresh and/or dried Folium mangiferae.
Described desalination preferably adopts ultrafiltration process or dialysis method, and ultrafiltration process employing molecular weight cut-off is the ultra-filtration membrane desalination of 1000-100000, and dialysis method employing molecular weight cut-off is the dialysis tubing desalination of 1000-100000.
The Mengiferin that the present invention obtains, detect through HPLC, content is more than 98.5%.
Compared with prior art, advantage of the present invention:
1, prior art is when carrying out the extraction of Mengiferin, pre-treatment is all generally for solvent with water or ethanol etc., by heating extraction by effective constituent stripping, and the present invention adopts high pressure extract, compare heating to extract, through High Pressure, make the composition such as organic acid, polyphenol, the Mengiferin energy fully stripping contained in Folium mangiferae, be beneficial to the process of subsequent technique, be beneficial to the yield improving activeconstituents.
2, Mengiferin poorly water-soluble, is soluble in rare alcohol, hot methyl alcohol and ethanol, is soluble in sig water; Usually the Mengiferin 7-O-glycoside (Mangiferin-7-0-R-D-glucopy-ranoside) with its amount 5% coexists in leaf.Because the hydroxyl acidity of 3 in structure is comparatively strong, therefore two compounds are all soluble in alkaline water liquid, and Mengiferin 7-0-glycoside also can be hydrolyzed into Mengiferin in alkaline water liquid, thus can improve yield and the purity of Mengiferin.The present invention adds basic solution when extracting, and fully can be separated Mengiferin and Mengiferin 7-O-glycoside, then by subsequent technique, makes Mengiferin 7-O-glycoside to be converted into Mengiferin, improve yield.
3, the Mengiferin content that prior art obtains generally is no more than 98%, the present invention adopts the combination of high pressure extract and aqueous two phase extraction technique, can enhance productivity greatly, prior art column chromatography resin used, for being generally non-polar resin, can only obtain the extract of Mengiferin content 40-50% through primary sorption; If employing polar resin, also can only obtain Mengiferin content about 50% through primary sorption, the Mengiferin wanting to obtain more high-content by 2 times or more Adsorptions, thus must add process time and cost.Aqueous two phase extraction technique is as a kind of novel isolation technique, have mild condition, easy and simple to handle, be easy to series of advantages such as amplifying, separating power is strong, and adopt combine with technique of the present invention, there is the raising of matter, content and the extraction efficiency of Mengiferin can be increased, compare column chromatography after water extraction or alcohol extracting, the time of the present invention is short, and Mengiferin content is high.
4, present invention, avoiding the use of a large amount of organic solvent such as dehydrated alcohol, methyl alcohol, environmental protection more.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
From Folium mangiferae, extract a method for Mengiferin, specifically comprise the steps:
1) Feedstock treating: fresh mango leaf is smashed;
2) high pressure extract: by the raw material after process, put into pressure pan, add the pure water of identical weight, be forced into 4kgf/cm
2, make its pressure uniform, and keep 10min, recover normal pressure, filter, obtain extracting solution;
3) be separated: aqueous two-phase extraction is carried out to Folium mangiferae, obtain the upper lower phase with extraction mutually of extraction, the double-aqueous phase system that aqueous two-phase extraction uses is made up of polyoxyethylene glycol, sodium hydroxide and water, the mass percent of polyoxyethylene glycol in described double-aqueous phase system is 20%, the mass percent of sodium hydroxide in described double-aqueous phase system is 10%, surplus is water, and the weight-average molecular weight of polyoxyethylene glycol is 1000-8000;
4) subsequent disposal: by step 3) phase in the extraction that obtains, ultrafiltration process employing molecular weight cut-off is ultra-filtration membrane desalination, the recrystallization of 1000-100000, by step 3) phase under the extraction that obtains, add and be equivalent to extract lower phase mass percent 10% sodium hydroxide, ultrafiltration process employing molecular weight cut-off is ultra-filtration membrane desalination, the recrystallization of 1000-100000;
5) merge dried acquisition thing and obtain Mengiferin.
Embodiment 2:
From Folium mangiferae, extract a method for Mengiferin, specifically comprise the steps:
1) Feedstock treating: dry Folium mangiferae is pulverized;
2) high pressure extract: by the raw material after process, put into pressure pan, add the pure water of identical weight, be forced into 4kgf/cm
2, make its pressure uniform, and keep 15min, recover normal pressure, filter, obtain extracting solution;
3) be separated: aqueous two-phase extraction is carried out to Folium mangiferae, obtain the upper lower phase with extraction mutually of extraction, the double-aqueous phase system that aqueous two-phase extraction uses is made up of polyoxyethylene glycol, sodium hydroxide and water, the mass percent of polyoxyethylene glycol in described double-aqueous phase system is 10%, the mass percent of sodium hydroxide in described double-aqueous phase system is 20%, surplus is water, and the weight-average molecular weight of polyoxyethylene glycol is 1000-8000;
4) subsequent disposal: by step 3) phase in the extraction that obtains, dialysis method employing molecular weight cut-off is dialysis tubing desalination, the recrystallization of 1000-100000, by step 3) phase under the extraction that obtains, add and be equivalent to extract lower phase mass percent 5% sodium hydroxide, dialysis method employing molecular weight cut-off is dialysis tubing desalination, the recrystallization of 1000-100000;
5) merge dried acquisition thing and obtain Mengiferin.
Embodiment 3:
From Folium mangiferae, extract a method for Mengiferin, specifically comprise the steps:
1) Feedstock treating: dry Folium mangiferae is pulverized;
2) high pressure extract: by the raw material after process, put into pressure pan, add the pure water of identical weight, be forced into 4kgf/cm
2, make its pressure uniform, and keep 12min, recover normal pressure, filter, obtain extracting solution;
3) be separated: aqueous two-phase extraction is carried out to Folium mangiferae, obtain the upper lower phase with extraction mutually of extraction, the double-aqueous phase system that aqueous two-phase extraction uses is made up of polyoxyethylene glycol, sodium hydroxide and water, the mass percent of polyoxyethylene glycol in described double-aqueous phase system is 15%, the mass percent of sodium hydroxide in described double-aqueous phase system is 15%, surplus is water, and the weight-average molecular weight of polyoxyethylene glycol is 1000-8000;
4) subsequent disposal: by step 3) phase in the extraction that obtains, ultrafiltration process employing molecular weight cut-off is ultra-filtration membrane desalination, the recrystallization of 1000-100000, by step 3) phase under the extraction that obtains, add and be equivalent to extract lower phase mass percent 10% sodium hydroxide, ultrafiltration process employing molecular weight cut-off is ultra-filtration membrane desalination, the recrystallization of 1000-100000;
5) merge dried acquisition thing and obtain Mengiferin.
Result:
Claims (2)
1. from Folium mangiferae, extract a method for Mengiferin, it is characterized in that:
Comprise the steps:
1) Feedstock treating: Folium mangiferae is pulverized or smashes;
2) high pressure extract: by the raw material after process, put into pressure pan, add the pure water of identical weight, be forced into 4kgf/cm
2, make its pressure uniform, and keep 10-15min, recover normal pressure, filter, obtain extracting solution;
3) be separated: aqueous two-phase extraction is carried out to Folium mangiferae, obtain the upper lower phase with extraction mutually of extraction, the double-aqueous phase system that aqueous two-phase extraction uses is made up of polyoxyethylene glycol, sodium hydroxide and water, the mass percent of described polyoxyethylene glycol in described double-aqueous phase system is 10-20%, the mass percent of described sodium hydroxide in described double-aqueous phase system is 10-20%, surplus is water, and the weight-average molecular weight of described polyoxyethylene glycol is 1000-8000;
4) subsequent disposal: by step 3) phase in the extraction that obtains, desalination, recrystallization, by step 3) phase under the extraction that obtains, add and be equivalent to extract lower phase mass percent 5-10% sodium hydroxide, desalination, recrystallization;
5) the acquisition thing after merging recrystallization obtains Mengiferin.
2. a kind of method extracting Mengiferin from Folium mangiferae according to claim 1, is characterized in that: described Folium mangiferae is fresh and/or dried Folium mangiferae.
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| CN201410619517.9A CN104311614B (en) | 2014-11-05 | 2014-11-05 | A kind of method extracting Mengiferin from Folium mangiferae |
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| CN201410619517.9A CN104311614B (en) | 2014-11-05 | 2014-11-05 | A kind of method extracting Mengiferin from Folium mangiferae |
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| CN104311614B CN104311614B (en) | 2016-07-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109438429A (en) * | 2018-12-29 | 2019-03-08 | 桂林医学院 | A method of extracting mangiferin from mango leaf |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109438429A (en) * | 2018-12-29 | 2019-03-08 | 桂林医学院 | A method of extracting mangiferin from mango leaf |
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