CN103920305A - Tank group type countercurrent cordyceps extracting method - Google Patents
Tank group type countercurrent cordyceps extracting method Download PDFInfo
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Abstract
The invention relates to an extracting method of effective components from cordyceps mycelia and in particular relates to a tank group type countercurrent cordyceps extracting method, which comprises the following steps: putting the cordyceps mycelia and alcohol in a tank group type countercurrent extracting device, wherein the concentration of the alcohol is 90-98% and the mass of the alcohol is 8-12 times that of the cordyceps mycelia; performing at least first circulation extraction, second circulation extraction and third circulation extraction, wherein each tank group includes three extracting tank which are jointly used and in serial connection, the countercurrent extracting flow speed is 25-35 min per tank and the extracting temperature is 30-80 DEG C; collecting to obtain primary extracting liquid; pre-treating to obtain pre-treated liquid; filtering out deposition and taking supernatant; reducing pressure and performing vacuum concentration on the supernatant to 1.25-1.35 specific gravity to obtain concentration liquid; finally performing spray drying on the concentration liquid to obtain power product. According to the extracting method, free sterols, cordycepin, adenosine and polysaccharide are effectively extracted from the cordyceps mycelia and on the premise that the extracting rate is not lowered, the usage amount of an extracting solvent can be reduce by above 2/3.
Description
Technical field
The present invention relates to a kind of from cordyceps mycelia the method for effective component extracting, relate in particular to the method for pot group type counter-flow extraction Chinese caterpillar fungus a kind of.
Background technology
Pot group type dynamic countercurrent extraction process, by plural dynamic extraction pot units connected in series, extract solvent and carry by each tank in turn from low to high inversely along the solute concentration gradient of each tank spice in tank group, and keep certain extraction time time to use with spice.This technology has mainly utilized the concentration gradient of active ingredient in solid-liquid (medicinal material and solvent) two-phase poor, step by step active ingredient in medicinal material is diffused in the extraction solvent that initial concentration is lower, reaches the object that shifts to greatest extent active ingredient in medicinal material.Adopt pot group type counter-flow extraction technique, can effectively utilize the concentration gradient of solid-liquid two-phase, increase concentration difference, extraction rate is fast, the Enrichment of extract, and extracting cycle is short, utilizes medicinal material body to make filtering layer in the time of extraction, can improve clarity.
CN101709024B (2012-11-28) discloses a kind of method of extracting purifying RV from giant knotweed, the method adds molysite with ethanol and alkali is to extract medium, adopt pot group type dynamic countercurrent extractive technique, extract makes RV product through steps such as desalination, purifying, acidifying, enzymolysis, concentrated, removal of impurities.Yet the method is not suitable for extracting Chinese caterpillar fungus active ingredient from cordyceps mycelia.
Summary of the invention
The method that the object of this invention is to provide the pot group type counter-flow extraction Chinese caterpillar fungus that a kind of solvent load is few, recovery rate is high.
Above-mentioned technical purpose of the present invention is achieved by the following technical programs:
A method for pot group type counter-flow extraction Chinese caterpillar fungus, it comprises the following steps:
(1) counter-flow extraction: by 100kg cordyceps mycelia and 8-12 doubly the alcohol of the 90-98% of described Chinese caterpillar fungus hypha weight be placed in pot group type counterflow extraction apparatus and comprise that three circulations of at least the first circulation, the second circulation and the 3rd circulation extract, described filling group is that every group of three extractors series connection share, counter-flow extraction flow velocity is 25-35min/ tank, and extraction temperature is 30-80 ℃;
Described the first circulation is since the first extractor counter-flow extraction: specifically by cordyceps mycelia and 8-12 doubly the alcohol of the 90-98% of described Chinese caterpillar fungus hypha weight by equal portions, add in the first extractor, the second extractor and the 3rd extractor respectively; After the medical filtration that the first extractor extracts for the first time, put into fluid reservoir standby, the extraction for the second time of the first extractor and the extraction for the third time of the first extractor add respectively 4-5 times of water; Then the solvent extracting for the first time using the second extract of the first extractor as the second extractor, the first extracting liquid filtering of the second extractor is standby; The solvent extracting for the second time using the 3rd extract of the first extractor as the second extractor, adds 4-5 times of water in the extraction for the second time of the second extractor; The second extract of the second extractor is as the solvent extracting for the first time of the 3rd extractor;
Described the second circulation is since the second extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the first circulation to the second extractor of the cordyceps mycelia slag after including the first circulation and extracting for the second time, then add 4-5 times of water to extract for the first time; In the 3rd extractor of the cordyceps mycelia slag using the first extract of the second extractor after including the first circulation and extracting for the first time, then add 4-5 times of water to extract for the first time; Then the first extract of the 3rd extractor is extracted in the first extractor of the cordyceps mycelia slag after including the first circulation and extracting for the third time for the first time;
Described the 3rd circulation is since the 3rd extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the second circulation to the 3rd extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time, then add 4-5 times of water to extract for the first time; In the first extractor of the cordyceps mycelia slag using the first extract of the 3rd extractor after including the second circulation and extracting for the first time, then add 4-5 times of water to extract for the first time; Then the first extract of the first extractor is extracted in the second extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time for the first time;
Described alcohol is one or more in methyl alcohol, ethanol or isopropyl alcohol;
(2) collect: merge the first extract in the first extractor, the second extractor and the 3rd extractor in the 3rd circulation, obtain just extract;
(3) pre-treatment: described just extract reduced vacuum is concentrated, then add the cooling standing 8-15 of deionized water hour and obtain pretreatment liquid;
(4) filter: described pretreatment liquid, with filtering filtering precipitation, is got to supernatant;
(5) concentrated: it is 1.25-1.35 that described supernatant reduced vacuum is concentrated into proportion, obtains concentrate;
(6) spraying is dry: by the dry powder-product that obtains of described concentrate spraying.
The present invention adopts specific counter-flow extraction, pre-treatment, filtration simultaneously, concentrated and spraying is dry, can effectively extract free sterol, cordycepin, adenosine and polysaccharide in cordyceps mycelia; Under the condition that does not reduce recovery rate, can reduce more than 2/3 extraction solvent consumption, and the combination ergosterol that makes to extract in raw material is effectively free, thereby has improved the extraction efficiency of free ergosterol.
Cordycepin is a kind of medicine of natural origin, has the multiple pharmacological effect such as antitumor, anti-bacteria and anti-virus, immunological regulation, removing free radical, has good potential applicability in clinical practice, especially multiple solid malignant is had to very strong inhibitory action.Therefore, the trend that is inevitable it as a kind of broad-spectrum new drug exploitation.Cordycepin has fat-soluble, and conventional extraction is to extract 3 times each 90 minutes in 80 ℃ of hot refluxs with the water of 10 times of amounts, but extraction efficiency is not high.If temperature surpasses 80 ℃ of cordycepins, be easy to destroyed.The polysaccharide that cordyceps mycelia polysaccharide is comprised of mannose, cordycepin, adenosine, galactolipin, arabinose, wood sugar essence, glucose, fucose.Experiment showed, that cordyceps mycelia polysaccharide can improve immune function of human body, increasing leukocyte, the clinical malignant tumour that has been used for the treatment of.Ergosterol is a kind of important phytosterol (seeing steroid), is terpenoid, molecular formula C
28h
44o.Be present in yeast and ergot.White plates or acicular crystal.168 ℃ of fusing points (containing the crystallization of 1.5 mole of water), 250 ℃ of boiling points (0.01 millimetres of mercury), specific rotatory power-135 ° (chloroform, C=1.2).Be dissolved in benzene and chloroform.Ergosterol irradiates through ultraviolet ray, and a series of variations occur, and generates calciferol; The derivative 22 of ergosterol, 23-dihydroergosterol changes vitamin(e) D4 into after ultraviolet ray is irradiated.
Ergosterol can transform and obtain calciferol in human body, and calciferol has critical function in the metabolism that regulates human body and animal, has the absorption effect of mineral matters such as promoting calcium, phosphorus, is widely used in nutraceutical, medicine trade.
As preferably, in described three circulation extractions, within every 8 hours, disconnect first extractor discharging, after again feeding intake, connect successively.
The extraction that repeatedly circulates, can effectively extract the active ingredient in cordyceps mycelia.If the time is too short, may extract not exclusively; If the time is oversize, extraction efficiency can not increase yet, and wastes on the contrary the costs such as solvent.
As preferably, described step (1) counter-flow extraction flow velocity is 30min/ tank, and extracting temperature is 70 ℃.
As preferably, described step (3) be that under-0.01～-0.03MPa, reduced vacuum is concentrated into 180-220kg by described just extract in vacuum, then adds deionized water 600-1000kg, cooling standing 8-15 hour must pretreatment liquid.
More preferably, described step (3) is that under-0.01～-0.03MPa, reduced vacuum is concentrated into 200kg by described just extract in vacuum, then adds deionized water 800kg, within cooling standing 8-15 hour, obtains pretreatment liquid.
As preferably, described step (5) is also included in described in concentrated forward direction and adds and account for the benzinum of supernatant 1.5-3 times quality and the mixed liquor of methyl alcohol in supernatant liquid, mixes and soaks 0.5-0.8h, ultrasonic extraction 2-4min under 40-50 ℃, 50-70KHz.
Adopt this technique can make the free ergosterol in Chinese caterpillar fungus raw material obtain more effective extraction, and the combination ergosterol that makes to extract in raw material is more effectively free, thereby further improves the extraction efficiency of free ergosterol; And be conducive to follow-up concentrated.
As preferably, described step (5) specifically by described supernatant vacuum be under-0.08～-0.095MPa reduced vacuum to be concentrated into proportion be 1.3, obtain concentrate.
By this Vacuum Concentration, make the free ergosterol in Chinese caterpillar fungus raw material obtain more effective extraction, and make that follow-up spraying is dry more easily to be carried out.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of pot group type counter-flow extraction Chinese caterpillar fungus of the present invention;
In figure, 1-the first extractor; 2-the second extractor; 3-the 3rd extractor.
The specific embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment mono-
As shown in Figure 1, pot group type counterflow extraction apparatus comprises the first extractor 1, the second extractor 2 and the 3rd extractor 3.The ethanol of 100kg cordyceps mycelia and 1000kg95% is added in the first extractor 1, the second extractor 2 and the 3rd extractor 3 by equal portions respectively; Three circulations that comprise at least the first circulation, the second circulation and the 3rd circulation are extracted, and filling group is that every group of three extractors series connection share, and counter-flow extraction flow velocity is 30min/ tank, and extracting temperature is 70 ℃.
The first circulation is since the first extractor counter-flow extraction: after the medical filtration that the first extractor extracts for the first time, put into fluid reservoir standby, the extraction for the second time of the first extractor and the extraction for the third time of the first extractor add respectively 4-5 times of water; Then the solvent extracting for the first time using the second extract of the first extractor as the second extractor, the first extracting liquid filtering of the second extractor is standby; The solvent extracting for the second time using the 3rd extract of the first extractor as the second extractor, adds 4-5 times of water in the extraction for the second time of the second extractor; The second extract of the second extractor is as the solvent extracting for the first time of the 3rd extractor;
The second circulation is since the second extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the first circulation to the second extractor of the cordyceps mycelia slag after including the first circulation and extracting for the second time, then add 450kg water to extract for the first time; In the 3rd extractor of the cordyceps mycelia slag using the first extract of the second extractor after including the first circulation and extracting for the first time, then add 450kg water to extract for the first time; Then the first extract of the 3rd extractor is extracted in the first extractor of the cordyceps mycelia slag after including the first circulation and extracting for the third time for the first time;
The 3rd circulation is since the 3rd extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the second circulation to the 3rd extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time, then add 450kg water to extract for the first time; In the first extractor of the cordyceps mycelia slag using the first extract of the 3rd extractor after including the second circulation and extracting for the first time, then add 450kg water to extract for the first time; Then the first extract of the first extractor is extracted in the second extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time for the first time;
Merge the first extract in the first extractor, the second extractor and the 3rd extractor in the 3rd circulation, obtain just extract; In concentrated forward direction supernatant liquid, add and account for the benzinum of supernatant 200kg and the mixed liquor of methyl alcohol (mass ratio 1:1), mix and soak 0.6h, ultrasonic extraction 3min under 46 ℃, 60KHz; By first extract in vacuum for reduced vacuum under-0.02MPa is concentrated into 200kg, then add deionized water 800kg, cooling standing 10 hours pretreatment liquid; Pretreatment liquid, with filtering filtering precipitation, is got to supernatant; By supernatant, in vacuum, for reduced vacuum under-0.09MPa is concentrated into proportion, be 1.3, obtain concentrate; By the dry Chinese caterpillar fungus extraction product that obtains of concentrate spraying.
If need to isolate different extracts, can adopt column chromatography and specificity check experiment to carry out separated, the recovery rate of different material Mass Calculation free sterol, cordycepin, adenosine and the polysaccharide then obtaining by separation.Concrete numerical value is in Table 1.
Embodiment bis-
As shown in Figure 1, pot group type counterflow extraction apparatus comprises the first extractor 1, the second extractor 2 and the 3rd extractor 3.90% methyl alcohol of cordyceps mycelia and 8 times of Chinese caterpillar fungus hypha weights is added in the first extractor 1, the second extractor 2 and the 3rd extractor 3 by equal portions respectively; Three circulations that comprise at least the first circulation, the second circulation and the 3rd circulation are extracted, and filling group is that every group of three extractors series connection share, and counter-flow extraction flow velocity is 25min/ tank, and extracting temperature is 30 ℃;
The first circulation is since the first extractor counter-flow extraction: after the medical filtration that the first extractor extracts for the first time, put into fluid reservoir standby, the extraction for the second time of the first extractor and the extraction for the third time of the first extractor add respectively 4 times of water; Then the solvent extracting for the first time using the second extract of the first extractor as the second extractor, the first extracting liquid filtering of the second extractor is standby; The solvent extracting for the second time using the 3rd extract of the first extractor as the second extractor, adds 4 times of water in the extraction for the second time of the second extractor; The second extract of the second extractor is as the solvent extracting for the first time of the 3rd extractor;
The second circulation is since the second extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the first circulation to the second extractor of the cordyceps mycelia slag after including the first circulation and extracting for the second time, then add 4 times of water to extract for the first time; In the 3rd extractor of the cordyceps mycelia slag using the first extract of the second extractor after including the first circulation and extracting for the first time, then add 4 times of water to extract for the first time; Then the first extract of the 3rd extractor is extracted in the first extractor of the cordyceps mycelia slag after including the first circulation and extracting for the third time for the first time;
The 3rd circulation is since the 3rd extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the second circulation to the 3rd extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time, then add 4 times of water to extract for the first time; In the first extractor of the cordyceps mycelia slag using the first extract of the 3rd extractor after including the second circulation and extracting for the first time, then add 4 times of water to extract for the first time; Then the first extract of the first extractor is extracted in the second extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time for the first time;
Merge the first extract in the first extractor, the second extractor and the 3rd extractor in the 3rd circulation, obtain just extract; In concentrated forward direction supernatant liquid, add and account for the benzinum of 1.5 times of quality of supernatant and the mixed liquor of methyl alcohol, mix and soak 0.5h, ultrasonic extraction 2min under 40 ℃, 50KHz; By first extract in vacuum for reduced vacuum under-0.01MPa is concentrated into 180kg, then add deionized water 600kg, cooling standing 8 hours pretreatment liquid; Pretreatment liquid, with filtering filtering precipitation, is got to supernatant; It is 1.25 that supernatant reduced vacuum is concentrated into proportion, obtains concentrate; By the dry Chinese caterpillar fungus extraction product that obtains of concentrate spraying.
Embodiment tri-
As shown in Figure 1, pot group type counterflow extraction apparatus comprises the first extractor 1, the second extractor 2 and the 3rd extractor 3.One or more in 98% isopropyl alcohol of cordyceps mycelia and 12 times of Chinese caterpillar fungus hypha weights are added in the first extractor 1, the second extractor 2 and the 3rd extractor 3 by equal portions respectively; Three circulations that comprise at least the first circulation, the second circulation and the 3rd circulation are extracted, and filling group is that every group of three extractors series connection share, and counter-flow extraction flow velocity is 35min/ tank, and extracting temperature is 80 ℃;
The first circulation is since the first extractor counter-flow extraction: after the medical filtration that the first extractor extracts for the first time, put into fluid reservoir standby, the extraction for the second time of the first extractor and the extraction for the third time of the first extractor add respectively 5 times of water; Then the solvent extracting for the first time using the second extract of the first extractor as the second extractor, the first extracting liquid filtering of the second extractor is standby; The solvent extracting for the second time using the 3rd extract of the first extractor as the second extractor, adds 5 times of water in the extraction for the second time of the second extractor; The second extract of the second extractor is as the solvent extracting for the first time of the 3rd extractor;
The second circulation is since the second extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the first circulation to the second extractor of the cordyceps mycelia slag after including the first circulation and extracting for the second time, then add 5 times of water to extract for the first time; In the 3rd extractor of the cordyceps mycelia slag using the first extract of the second extractor after including the first circulation and extracting for the first time, then add 5 times of water to extract for the first time; Then the first extract of the 3rd extractor is extracted in the first extractor of the cordyceps mycelia slag after including the first circulation and extracting for the third time for the first time;
The 3rd circulation is since the 3rd extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the second circulation to the 3rd extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time, then add 5 times of water to extract for the first time; In the first extractor of the cordyceps mycelia slag using the first extract of the 3rd extractor after including the second circulation and extracting for the first time, then add 5 times of water to extract for the first time; Then the first extract of the first extractor is extracted in the second extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time for the first time;
Merge the first extract in the first extractor, the second extractor and the 3rd extractor in the 3rd circulation, obtain just extract; In concentrated forward direction supernatant liquid, add and account for the benzinum of 3 times of quality of supernatant and the mixed liquor of methyl alcohol, mix and soak 0.8h, ultrasonic extraction 4min under 50 ℃, 70KHz; By first extract in vacuum for reduced vacuum under-0.03MPa is concentrated into 220kg, then add deionized water 1000kg, cooling standing 15 hours pretreatment liquid; Pretreatment liquid, with filtering filtering precipitation, is got to supernatant; It is 1.35 that supernatant reduced vacuum is concentrated into proportion, obtains concentrate; By the dry Chinese caterpillar fungus extraction product that obtains of concentrate spraying.
Embodiment tetra-
With embodiment mono-, different is that three circulation extraction circulations constantly repeat to continuous 8 hours, within every 8 hours, disconnects first extractor discharging, after again feeding intake, connects successively.
Comparative example one
With embodiment mono-, the different counter-flow extraction processes of only having the first circulation while being counter-flow extraction, do not comprise the extraction of the second circulation and the 3rd circulation, so its first extract is the first extract in the first extractor, the second extractor and the 3rd extractor in the first circulation.
Table 1 embodiment and comparative example's data comparison
This specific embodiment is only explanation of the invention; it is not limitation of the present invention; those skilled in the art can make to the present embodiment the modification that there is no creative contribution as required after reading this description, but as long as within the scope of claim of the present invention, are all subject to the protection of Patent Law.
Claims (7)
1. a method for pot group type counter-flow extraction Chinese caterpillar fungus, is characterized in that: it comprises the following steps:
(1) counter-flow extraction: by 100kg cordyceps mycelia and 8-12 doubly the alcohol of the 90-98% of described Chinese caterpillar fungus hypha weight be placed in pot group type counterflow extraction apparatus and comprise that three circulations of at least the first circulation, the second circulation and the 3rd circulation extract, described filling group is that every group of three extractors series connection share, counter-flow extraction flow velocity is 25-35min/ tank, and extraction temperature is 30-80 ℃;
Described the first circulation is since the first extractor counter-flow extraction: specifically by cordyceps mycelia and 8-12 doubly the alcohol of the 90-98% of described Chinese caterpillar fungus hypha weight by equal portions, add in the first extractor, the second extractor and the 3rd extractor respectively; After the medical filtration that the first extractor extracts for the first time, put into fluid reservoir standby, the extraction for the second time of the first extractor and the extraction for the third time of the first extractor add respectively 4-5 times of water; Then the solvent extracting for the first time using the second extract of the first extractor as the second extractor, the first extracting liquid filtering of the second extractor is standby; The solvent extracting for the second time using the 3rd extract of the first extractor as the second extractor, adds 4-5 times of water in the extraction for the second time of the second extractor; The second extract of the second extractor is as the solvent extracting for the first time of the 3rd extractor;
Described the second circulation is since the second extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the first circulation to the second extractor of the cordyceps mycelia slag after including the first circulation and extracting for the second time, then add 4-5 times of water to extract for the first time; In the 3rd extractor of the cordyceps mycelia slag using the first extract of the second extractor after including the first circulation and extracting for the first time, then add 4-5 times of water to extract for the first time; Then the first extract of the 3rd extractor is extracted in the first extractor of the cordyceps mycelia slag after including the first circulation and extracting for the third time for the first time;
Described the 3rd circulation is since the 3rd extractor counter-flow extraction: specifically merge the first extract in the first extractor, the second extractor and the 3rd extractor in the second circulation to the 3rd extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time, then add 4-5 times of water to extract for the first time; In the first extractor of the cordyceps mycelia slag using the first extract of the 3rd extractor after including the second circulation and extracting for the first time, then add 4-5 times of water to extract for the first time; Then the first extract of the first extractor is extracted in the second extractor of the cordyceps mycelia slag after including the second circulation and extracting for the first time for the first time;
Described alcohol is one or more in methyl alcohol, ethanol or isopropyl alcohol;
(2) collect: merge the first extract in the first extractor, the second extractor and the 3rd extractor in the 3rd circulation, obtain just extract;
(3) pre-treatment: described just extract reduced vacuum is concentrated, then add the cooling standing 8-15 of deionized water hour and obtain pretreatment liquid;
(4) filter: described pretreatment liquid, with filtering filtering precipitation, is got to supernatant;
(5) concentrated: it is 1.25-1.35 that described supernatant reduced vacuum is concentrated into proportion, obtains concentrate;
(6) spraying is dry: by the dry powder-product that obtains of described concentrate spraying.
2. the method for a kind of pot group type counter-flow extraction Chinese caterpillar fungus according to claim 1, is characterized in that: described three circulations disconnect first extractor discharging in every 8 hours in extracting, and after again feeding intake, connect successively.
3. the method for a kind of pot group type counter-flow extraction Chinese caterpillar fungus according to claim 1, is characterized in that: described step (1) counter-flow extraction flow velocity is 30min/ tank, and extracting temperature is 70 ℃.
4. the method for a kind of pot group type counter-flow extraction Chinese caterpillar fungus according to claim 3, it is characterized in that: described step (3) is that under-0.01～-0.03MPa, reduced vacuum is concentrated into 180-220kg by described just extract in vacuum, then add deionized water 600-1000kg, within cooling standing 8-15 hour, obtain pretreatment liquid.
5. the method for a kind of pot group type counter-flow extraction Chinese caterpillar fungus according to claim 4, it is characterized in that: described step (3) is that under-0.01～-0.03MPa, reduced vacuum is concentrated into 200kg by described just extract in vacuum, then add deionized water 800kg, within cooling standing 8-15 hour, obtain pretreatment liquid.
6. according to the method for a kind of pot group type counter-flow extraction Chinese caterpillar fungus described in claim 2 or 3 or 4, it is characterized in that: described step (5) is also included in described in concentrated forward direction and adds and account for the benzinum of supernatant 1.5-3 times quality and the mixed liquor of methyl alcohol in supernatant liquid, mix to soak 0.5-0.8h, ultrasonic extraction 2-4min under 40-50 ℃, 50-70KHz.
7. the method for a kind of pot group type counter-flow extraction Chinese caterpillar fungus according to claim 6, is characterized in that: described step (5) specifically by described supernatant vacuum be under-0.08～-0.095MPa reduced vacuum to be concentrated into proportion be 1.3, obtain concentrate.
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| CN101709024A (en) * | 2009-11-06 | 2010-05-19 | 淮阴工学院 | Method for extracting and purifying resveratrol from polygonum cuspidatum |
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| JPH07115906A (en) * | 1993-10-29 | 1995-05-09 | Mitsubishi Heavy Ind Ltd | Extraction of drink and apparatus therefor |
| CN101199595A (en) * | 2006-12-13 | 2008-06-18 | 中国药材集团公司 | Extractive process of baical skullcap root active ingredient |
| CN101709024A (en) * | 2009-11-06 | 2010-05-19 | 淮阴工学院 | Method for extracting and purifying resveratrol from polygonum cuspidatum |
Cited By (7)
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| CN105481872A (en) * | 2015-12-01 | 2016-04-13 | 刘寒毅 | Method for extracting plant active ingredients by constant temperature gradient in-series extraction |
| CN105797082A (en) * | 2016-05-11 | 2016-07-27 | 成都蓉泽缘食品有限公司 | Preparation method of kidney tonifying traditional Chinese medicine |
| CN105998995A (en) * | 2016-05-11 | 2016-10-12 | 成都蓉泽缘食品有限公司 | Kidney tonifying preparation |
| US11230613B2 (en) | 2017-08-08 | 2022-01-25 | Kultevat, Inc. | Rubber and by-product extraction systems and methods |
| US11565195B2 (en) | 2017-08-08 | 2023-01-31 | Kultevat, Inc. | System and method for continuous stirred tank solvent extraction using feedstock |
| US11566086B2 (en) | 2017-08-08 | 2023-01-31 | Kultevat, Inc. | Rubber and by-product extraction systems and methods |
| CN110024894A (en) * | 2019-05-14 | 2019-07-19 | 杭州民泰中药饮片有限公司 | A kind of preparation process of integration of drinking and medicinal herbs alternative tea |
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|---|---|
| CN103920305B (en) | 2015-12-02 |
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