CN101993459A - Process for extraction separation of bamboo leaf flavonoids by two aqueous phases - Google Patents
Process for extraction separation of bamboo leaf flavonoids by two aqueous phases Download PDFInfo
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- CN101993459A CN101993459A CN2009100442044A CN200910044204A CN101993459A CN 101993459 A CN101993459 A CN 101993459A CN 2009100442044 A CN2009100442044 A CN 2009100442044A CN 200910044204 A CN200910044204 A CN 200910044204A CN 101993459 A CN101993459 A CN 101993459A
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- bamboo leaf
- leaf flavonoids
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Abstract
The invention discloses a process for the extraction separation of bamboo leaf flavonoids by two aqueous phases. For overcoming the drawbacks of complex operation, time consumption, low extraction rate and low quality of extracted bamboo leaf flavonoids and the like of the conventional method for extracting bamboo leaf flavonoids from bamboo leaves, the processes comprises: adding ethanol into bamboo leaf powder, filtering, concentrating filtrate, recovering ethanol, and obtaining concentrated solution; centrifuging the concentrated solution and collecting supernate; adding polyethylene glycol and glucan into the supernate, uniformly mixing, centrifuging and collecting a glucan phase; and separating glucan by a conventional method and obtaining bamboo leaf flavonoids. The method is used for extracting bamboo leaf flavonoids from the bamboo leaves can amplify a project and realize continuous operation and is simple, high in extraction rate, large in handling capacity, low in energy consumption and production cost, free from arousing inactivation or denaturation of bamboo leaf flavonoids, short in phase separation time, small in interface tension and free from organic solvent residue.
Description
Technical field
The present invention relates to a kind of technology of from the leaf of bamboo, extracting Folium Bambosae flavone, be specially a kind of aqueous two-phase extraction and separate Folium Bambosae flavone technology.
Background technology
Main effective constituent in the leaf of bamboo is Folium Bambosae flavone.Anti-oxidant, antibiotic and antiviral effect that Folium Bambosae flavone has is a kind of have heat-clearing toxin-expelling functions and very promising antioxidant.
Folium Bambosae flavone mainly exists with the form of carbon glycosyl.The existing method of extracting Folium Bambosae flavone from the leaf of bamboo generally adopts water, solvent extraction that the aqueous ethanolic solution isopolarity is big, or adopts the macroporous adsorbent resin method to extract.The weak point of these methods is, complex operation, time-consuming, extraction yield is low, the Folium Bambosae flavone quality extracted is not high.
Summary of the invention
Purpose of the present invention will overcome above-mentioned deficiency exactly, provide a kind of treatment capacity big, energy consumption is low, production cost is low, do not cause Folium Bambosae flavone inactivation or sex change, the phase-splitting time is short, interfacial tension is little, do not have organic solvent residual, sepn process is more economical, be easy to the engineering amplification separates Folium Bambosae flavone technology with the aqueous two-phase extraction of operate continuously.
For achieving the above object, technical scheme of the present invention is that this aqueous two-phase extraction separates Folium Bambosae flavone technology and is:
(1) in bamboo leaf powder, add mass ratio 70% ethanol 140-160ml, 100 orders filter ,-the 0.08-0.1MPa concentrated filtrate, reclaim ethanol, concentrated solution;
(2) the concentrated solution 2000rpm with described step a is centrifugal, collects supernatant liquor;
(3) in the supernatant liquor of described step b, add polyoxyethylene glycol (PEG) and 8% dextran of mass ratio 10-12%, regulate pH to 7-9, mix, 2000rpm is centrifugal, collection dextran phase (following phase);
(4) with the conventional mutually dextran of separating of the dextran of described step c, get Folium Bambosae flavone (content can reach more than 80%).
The present invention is behind refluxing extraction, centrifugal, aqueous two-phase extraction, and it is concentrated and purified that Folium Bambosae flavone is able to, and obtains high-quality Folium Bambosae flavone.The present invention utilizes the selectivity of Folium Bambosae flavone in polyoxyethylene glycol (PEG)/dextran aqueous two-phase system to distribute, and after Folium Bambosae flavone enters double-aqueous phase system, distributes in the two alternate selectivity of carrying out, and shows certain partition ratio.Folium Bambosae flavone has affinity to dextran, make Folium Bambosae flavone can more easily be dissolved in the following phase that is rich in dextran, and some impurity is distributed in phase.
Experiment shows that the present invention adopts the aqueous two-phase extraction method, and is centrifugal by whizzer, can reach the effect of quick phase-splitting, and phase-splitting is rapid, has shortened the time; The aqueous two-phase extraction method has been stayed a large amount of impurity in last phase polyoxyethylene glycol (PEG) polymkeric substance, flavonoid compound enters down phase (dextran), make Folium Bambosae flavone and impurity obtain effectively separating, obtained high-quality Folium Bambosae flavone, guaranteed the stability of Folium Bambosae flavone, can be and cause Folium Bambosae flavone inactivation or sex change because of external environments such as temperature.The partition ratio of Folium Bambosae flavone under top condition is 0.125, and yield reaches 0.868.
The present invention is used for extracting Folium Bambosae flavone from the leaf of bamboo, treatment capacity is big, and energy consumption is low, production cost is low, do not cause Folium Bambosae flavone inactivation or sex change, the phase-splitting time is short, interfacial tension is little, do not have organic solvent residual, sepn process economy, be easy to engineering and amplify and operate continuously.
Embodiment
Embodiment 1
(1) in the leaf of bamboo of impurity elimination, cleaning, oven dry, pulverizing, add mass ratio 70% ethanol 150ml, 90 ℃ of refluxing extraction 2 times, each 2h, 100 orders filter, merging filtrate ,-0.09MPa concentrated filtrate reclaims ethanol, concentrated solution.
(2) concentrated solution is placed whizzer, centrifugal 5min under the rotating speed of 2000r/min, it is standby to collect supernatant liquor.
(3) by this supernatant liquor, take by weighing the polyoxyethylene glycol of mass ratio 11% and 8% dextran, mix, add this supernatant liquor, regulate pH7-9, mix, centrifugal (dextran phase (following phase) is collected in 2000r/min, 5min) phase-splitting;
(4) with the conventional mutually dextran of separating of the dextran of described step c, get Folium Bambosae flavone.
Embodiment 2:
(1) in the leaf of bamboo of impurity elimination, cleaning, oven dry, pulverizing, add mass ratio 70% ethanol 140ml, 90 ℃ of refluxing extraction 2 times, each 2h, 100 orders filter, merging filtrate ,-0.08MPa concentrated filtrate reclaims ethanol, concentrated solution.
(2) concentrated solution is placed whizzer, the centrifugal 5min of 2000r/min, it is standby to collect supernatant liquor.
(3) by this supernatant liquor, take by weighing the polyoxyethylene glycol of mass ratio 10% and 8% dextran, mix, add this supernatant liquor, regulate pH7-9, mix, centrifugal (dextran phase (following phase) is collected in 2000r/min, 5min) phase-splitting;
(4) with the conventional mutually dextran of separating of the dextran of described step c, get Folium Bambosae flavone.
Embodiment 3:
(1) in the leaf of bamboo of impurity elimination, cleaning, oven dry, pulverizing, add mass ratio 70% ethanol 160ml, 90 ℃ of refluxing extraction 2 times, each 2h, 100 orders filter, merging filtrate ,-0.1MPa concentrated filtrate reclaims ethanol, concentrated solution.
(2) concentrated solution is placed whizzer, centrifugal 5min under the rotating speed of 2000r/min, it is standby to collect supernatant liquor.
(3) by this supernatant liquor, take by weighing the polyoxyethylene glycol of mass ratio 12% and 8% dextran, mix, join in this supernatant liquor, regulate pH7-9, mix, centrifugal (dextran phase (following phase) is collected in 2000r/min, 5min) phase-splitting;
(4) with the conventional mutually dextran of separating of the dextran of described step c, get Folium Bambosae flavone.
Claims (1)
1. an aqueous two-phase extraction separates Folium Bambosae flavone technology, it is characterized in that:
(1) in bamboo leaf powder, add mass ratio 70% ethanol 140-160ml, filter ,-the 0.08-0.1MPa concentrated filtrate, reclaim ethanol, concentrated solution;
(2) the concentrated solution 2000rpm with described step a is centrifugal, collects supernatant liquor;
(3) add the polyoxyethylene glycol of mass ratio 10-12% in the supernatant liquor of described step b, 8% dextran is regulated pH to 7-9, mixes, 2000rpm is centrifugal, collects the dextran phase;
(4) with the conventional mutually dextran of separating of the dextran of described step c, get Folium Bambosae flavone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910044204 CN101993459B (en) | 2009-08-24 | 2009-08-24 | Process for extraction separation of bamboo leaf flavonoids by two aqueous phases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910044204 CN101993459B (en) | 2009-08-24 | 2009-08-24 | Process for extraction separation of bamboo leaf flavonoids by two aqueous phases |
Publications (2)
| Publication Number | Publication Date |
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| CN101993459A true CN101993459A (en) | 2011-03-30 |
| CN101993459B CN101993459B (en) | 2013-04-17 |
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| CN 200910044204 Expired - Fee Related CN101993459B (en) | 2009-08-24 | 2009-08-24 | Process for extraction separation of bamboo leaf flavonoids by two aqueous phases |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911030A (en) * | 2011-08-02 | 2013-02-06 | 苏州宝泽堂医药科技有限公司 | Aqueous two-phase extraction method of angelica keiskei chalcone |
| CN103082036A (en) * | 2012-12-31 | 2013-05-08 | 浙江工业大学 | Oil-soluble bamboo leaf flavonoid derivative and application thereof |
| CN107596157A (en) * | 2017-09-18 | 2018-01-19 | 广西科技师范学院 | A kind of extracting method of bamboo-leaves flavones |
| CN112136044A (en) * | 2018-02-15 | 2020-12-25 | 耶迪特普大学 | Method for separating foreign bodies by means of a two-phase fluid system |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1543860A (en) * | 2003-11-14 | 2004-11-10 | 白栓锁 | Method for extracting bamboo leaf flavonoid and foodstuff and drug containing the same |
| CN1907994A (en) * | 2006-08-25 | 2007-02-07 | 胡林福 | Production method of extracting bamboo flavanone from bamboo leaf |
-
2009
- 2009-08-24 CN CN 200910044204 patent/CN101993459B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102911030A (en) * | 2011-08-02 | 2013-02-06 | 苏州宝泽堂医药科技有限公司 | Aqueous two-phase extraction method of angelica keiskei chalcone |
| CN103082036A (en) * | 2012-12-31 | 2013-05-08 | 浙江工业大学 | Oil-soluble bamboo leaf flavonoid derivative and application thereof |
| CN107596157A (en) * | 2017-09-18 | 2018-01-19 | 广西科技师范学院 | A kind of extracting method of bamboo-leaves flavones |
| CN112136044A (en) * | 2018-02-15 | 2020-12-25 | 耶迪特普大学 | Method for separating foreign bodies by means of a two-phase fluid system |
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| CN101993459B (en) | 2013-04-17 |
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