CN101429222A - Method for extracting mangiferin - Google Patents
Method for extracting mangiferin Download PDFInfo
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- CN101429222A CN101429222A CNA2007100663546A CN200710066354A CN101429222A CN 101429222 A CN101429222 A CN 101429222A CN A2007100663546 A CNA2007100663546 A CN A2007100663546A CN 200710066354 A CN200710066354 A CN 200710066354A CN 101429222 A CN101429222 A CN 101429222A
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- mangiferin
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Abstract
The invention relates to a method for extracting mangiferin, which uses a mango leaf or an almond leaf as a raw material to sufficiently extract the mangiferin in the mango leaf or the almond leaf by using water or alcohol-water mixed solution as menstruum through adopting a high-temperature and high-pressure method. The method has the advantages that the method has a simple process, saves energy and time, and is easy to realize industrialized production.
Description
Technical field
The present invention relates to a kind of technology of from Folium mangiferae or almond leaf, extracting Mangiferin.
Background technology
Guangxi is the big province of plantation mango, and the mango cultivated area reaches more than 40,000 hectare, accounts for 33% of national cultivated area.Can gather in the crops Folium mangiferae 100Kg by every mu and calculate, produce more than 60,000 ton of Folium mangiferae per year.This is the valuable source of Guangxi characteristic Chinese medicine industry, but all the time, these leaves are not fully utilized more than 90% except using as the prepared slices of Chinese crude drugs on a small quantity, have wasted.Adopt new technology and extract Mangiferin, can make full use of the characteristic agricultural byproducts resource in Guangxi, greatly improve the utilization ratio of Folium mangiferae, increase its added value, turn waste into wealth, for increasing peasant income, cut the waste, create conservation-minded society and contribute from Folium mangiferae.
Mangiferin is except having antibechic, Zhichuan, helping digestion, and modern study is found: Mangiferin also has functions such as antitumor, hypoglycemic, reducing blood-fat, brain tonic.
The chemical ingredients of Folium mangiferae and Study on extraction aspect, domestic have a few experiments research.Folium mangiferae contains xitix 237mg, tannin 11.73, multiple compositions such as Mangiferin 6.9 and ellagic acid, d one cachou extract, thick grass essence, quinic acid, kaempferol, c-a one thujene; EL-sissi etc. are separated to compositions such as Mangiferin, Quercetin, homomangiferin, Protocatechuic Acid, gallic acid from Folium mangiferae and chromatography has detected gallic acid, methyl gallate and Isomangiferin.Zhou Peichun etc. with Folium mangiferae make that decocting liquid, poach alcohol are molten, total glycosides etc., isolate the Mangiferin of certain antibechic, phlegm-dispelling functions.Lu Zhongyi etc., filter to get filtrate and filter residue through 95% alcohol reflux, concentrated with the Folium mangiferae meal.Filter residue is with 50 ethanol and gac recrystallization, and through polymeric amide dried bean noodles post low pressure chromatography, methyl alcohol is eluent, merges Rf and be 0.4 fluorescence spot part, evaporate to dryness, and recrystallization gets Mangiferin.Think the elegant tinkling of pieces of jade etc. and use 95% ethanol percolate extraction, decompression recycling ethanol, thick paste are used sherwood oil, ethyl acetate extraction successively, reclaim solvent and obtain Petroleum ether extraction portion, ethyl acetate extraction portion, water layer throw out.Wherein the water layer throw out is carried with 55% ethanol heat, and cooling obtains yellow needle crystal, obtains Mangiferin through purification process repeatedly.Application of air Research on Blasting Mangiferin extraction processes such as Huanghai Sea shore, effect is remarkable in the experiment of Mangiferin diacolation.Liu Qing etc. use water as solvent and extract Mangiferin from Folium mangiferaes, and with the definite factor that influences the Mangiferin extraction yield of orthogonal design, selecting 50 ℃ of water is solvent, investigates the influence of different frequency ultrasonic wave to the Mangiferin extraction yield.Found that ultrasonic frequency has remarkably influenced to extraction yield.
Patent of invention " mango general glycoside preparation and production method thereof " (patent (application) number: 03128247.4) disclose a kind of Chinese medicine preparation, the Mangiferin of its main component for from Folium mangiferae, extracting with preventing phlegm from forming and stopping coughing effect.(patent (application) number: 003125270.2), the disclosed method of extracting the total glycosides of mango from Folium mangiferae is patent of invention " Folium mangiferae total glycosides cough relief traditional Chinese medicine preparation and production method thereof ": Folium mangiferae or the pulverizing of almond leaf, alcohol reflux, non-polar solvent extraction, decolouring, filtration, crystallization etc.(patent (application) number: 200510093658.2), disclosed method is extracted 5 ~ 120Min for adopting ultrasonic wave or microwave down at 40 ~ 100 ℃ to patent of invention " a kind of method of extracting Mangiferin ".Patent of invention " preparation method of high purity mangiferin " (patent (application) number: 200610079234.5), adopt alcohol-water mixed liquid refluxing extraction repeatedly, go up column purification then, crystallization obtains Mangiferin.
The problem that existing disclosed Mangiferin extraction process exists is: 1, adopt chemical solvents to extract, exist environmental pollution and hazardous solvent residual, influence quality product and people's health; 2, extraction time longer, waste energy and increase cost.3, extraction is abundant inadequately.
Summary of the invention
The present invention mainly solves the problem that exists in the above-mentioned existing Mangiferin extractive technique, shortens extraction time, improves yield, save energy, minimizing environmental pollution.
Technical scheme of the present invention is:
The present invention with the mango leaf or partially peach leaves be raw material, through the high temperature solvent extract, concentrate, the Mangiferin purity that step obtained such as separation and purification reaches more than 60%.
Extracting method of the present invention is that the high heat of high pressure is extracted.Temperature is 101 ℃ ~ 374 ℃, and pressure is 3MPa ~ 60MPa.
Water of the present invention or aqueous solution of alcohol are the mixed solution of methyl alcohol, ethanol, propyl alcohol or butanols and water.Water is 30 ~ 100:70 ~ 0 with the blending ratio of alcohol.
Concentration method of the present invention is a membrane concentration.Concentration after concentrating is below 30%.
The present invention adopts column separating purification, and its resin that adopts is a macroporous resin, and model is D101, D301, D130 etc.
The present invention compared with prior art, its outstanding substantive distinguishing features and obvious improvement is:
1, extraction time shortens, save energy and other costs.The number of patent application that can mention according to background technology: 200510093658.2 and 200610079234.5 described extracting method, all need multiple extraction, its each extraction time is generally 30-120min, extraction yield is more than 90%, and extraction time of the present invention is generally below the 30min, and only need once to extract, extraction yield is more than 90%.
2, do not exist environmental pollution and hazardous solvent residual, the problem that influences quality product and be detrimental to health.
Embodiment
Embodiment 1
Fresh Folium mangiferae dries, and removes impurity, pulverizes, and in the extractor of packing into, adds deionized water with 1:3, mixes, and pressurization 20MPA heats 200 ℃, kept 20 minutes, during constantly stirring; Decompression, cooling.Solution is leached, cross membrane concentration, the concentration after concentrating is below 30%, and last model is the macroporous resin column of D101, wash-out, evaporation concentration, crystallization is separated out, dry finished product.
Embodiment 2
Fresh Folium mangiferae dries, and removes impurity, pulverizes, and in the extractor of packing into, adds alcohol-water mixture with 1:3, and wherein ethanol content 10%, mixes, and pressurization 30MPA heats 250 ℃, kept 30 minutes, during constantly stirring; Decompression, cooling.Solution is leached, cross membrane concentration, the concentration after concentrating is below 30%, and last model is the macroporous resin column of D301, wash-out, evaporation concentration, crystallization is separated out, dry finished product.
Embodiment 3
Fresh almond leaf dries, and removes impurity, pulverizes, and in the extractor of packing into, adds alcohol-water mixture with 1:3, and wherein propyl alcohol content 30%, mixes, and pressurization 40MPA heats 300 ℃, kept 15 minutes, during constantly stirring; Decompression, cooling.Solution is leached, cross membrane concentration, the concentration after concentrating is below 30%, and last model is the macroporous resin column of D130, wash-out, evaporation concentration, crystallization is separated out, dry finished product.
Embodiment 4
Gather fresh almond leaf, clean, remove impurity, pulverize, in the extractor of packing into, add alcohol-water mixture with 1:3, wherein propyl alcohol content 30%, mixes, and pressurization 40MPA heats 300 ℃, kept 15 minutes, during constantly stirring; Decompression, cooling.Solution is leached, cross membrane concentration, the concentration after concentrating is below 30%, and last model is the macroporous resin column of D101, wash-out, evaporation concentration, crystallization is separated out, dry finished product.
Claims (4)
1. a Mangiferin extraction process is characterized in that with mango leaf or almond leaf be raw material, through the high temperature solvent extract, concentrate, the Mangiferin purity that step obtained such as separation and purification reaches more than 60%; Described extraction step is that the high heat of high pressure is extracted, and extracting temperature is 101 ℃ ~ 374 ℃, and pressure is 3MPa ~ 60MPa, and the time is 3 ~ 120min.
2. extract the method for Mangiferin according to claim 1, it is characterized in that: described solvent is water or aqueous solution of alcohol, and aqueous solution of alcohol is the mixed solution of methyl alcohol, ethanol, propyl alcohol or butanols and water, and water is 30 ~ 100:70 ~ 0 with the blending ratio of alcohol.
3. extract the method for Mangiferin according to claim 1, it is characterized in that: described concentration method is a membrane concentration.
4. extract the method for Mangiferin according to claim 1, it is characterized in that: adopt column separating purification, its resin that adopts is a macroporous resin, and model is D101, D301, D130 etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100663546A CN101429222A (en) | 2007-11-05 | 2007-11-05 | Method for extracting mangiferin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100663546A CN101429222A (en) | 2007-11-05 | 2007-11-05 | Method for extracting mangiferin |
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| CN101429222A true CN101429222A (en) | 2009-05-13 |
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| CNA2007100663546A Pending CN101429222A (en) | 2007-11-05 | 2007-11-05 | Method for extracting mangiferin |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102008572A (en) * | 2010-10-28 | 2011-04-13 | 陕西科技大学 | Method for preparing effective ingredient extracts of peach leaves |
| CN102408415A (en) * | 2010-09-21 | 2012-04-11 | 昆明制药集团股份有限公司 | Preparation method of mangiferin |
| CN101993437B (en) * | 2009-08-27 | 2012-07-25 | 上海新康制药厂 | Method for extracting mangiferin from mango leaf |
| CN102617669A (en) * | 2012-03-03 | 2012-08-01 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN101993438B (en) * | 2009-08-27 | 2014-04-09 | 上海新康制药厂 | Method for extracting mangiferin and total saponins of rhizoma anemarrhenae from rhizoma anemarrhenae |
| CN103768112A (en) * | 2012-10-26 | 2014-05-07 | 昆明制药集团股份有限公司 | Mango leaf extract and application thereof |
| CN104311614A (en) * | 2014-11-05 | 2015-01-28 | 桂林益元素生物科技有限公司 | Method for extracting mangiferin from mango leaves |
| CN107048471A (en) * | 2017-05-08 | 2017-08-18 | 广西中烟工业有限责任公司 | A kind of preparation method of mango leaf extract and its application in cigarette |
| CN107383130A (en) * | 2017-07-20 | 2017-11-24 | 广西南宁桂知科技有限公司 | A kind of method that mangiferin is prepared using myrica rubra leaf |
| CN108239079A (en) * | 2016-12-23 | 2018-07-03 | 中国医学科学院药物研究所 | Mangiferin V type substance of crystalline substance and preparation method and its composition and purposes |
| CN108872440A (en) * | 2018-08-11 | 2018-11-23 | 中山市永恒化工新材料研究院有限公司 | The measurement method of phenol content in a kind of water |
| CN109081859A (en) * | 2018-09-27 | 2018-12-25 | 那坡康正天然植物提取有限责任公司 | The production method of mangiferin |
| CN109776515A (en) * | 2019-03-29 | 2019-05-21 | 广西工业职业技术学院 | The method of mangiferin is extracted from myrica rubra leaf |
| CN109912582A (en) * | 2019-03-29 | 2019-06-21 | 广西工业职业技术学院 | The method of mangiferin is extracted from mango leaf |
| CN113416182A (en) * | 2021-05-28 | 2021-09-21 | 西安金泰生物技术有限公司 | Mangiferin and preparation method thereof |
-
2007
- 2007-11-05 CN CNA2007100663546A patent/CN101429222A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993437B (en) * | 2009-08-27 | 2012-07-25 | 上海新康制药厂 | Method for extracting mangiferin from mango leaf |
| CN101993438B (en) * | 2009-08-27 | 2014-04-09 | 上海新康制药厂 | Method for extracting mangiferin and total saponins of rhizoma anemarrhenae from rhizoma anemarrhenae |
| CN102408415A (en) * | 2010-09-21 | 2012-04-11 | 昆明制药集团股份有限公司 | Preparation method of mangiferin |
| CN102408415B (en) * | 2010-09-21 | 2014-04-09 | 昆明制药集团股份有限公司 | Preparation method of mangiferin |
| CN102008572A (en) * | 2010-10-28 | 2011-04-13 | 陕西科技大学 | Method for preparing effective ingredient extracts of peach leaves |
| CN102617669B (en) * | 2012-03-03 | 2014-06-04 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN102617669A (en) * | 2012-03-03 | 2012-08-01 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN103768112B (en) * | 2012-10-26 | 2016-09-07 | 昆药集团股份有限公司 | A kind of mango leaf extract and application thereof |
| CN103768112A (en) * | 2012-10-26 | 2014-05-07 | 昆明制药集团股份有限公司 | Mango leaf extract and application thereof |
| CN104311614B (en) * | 2014-11-05 | 2016-07-20 | 桂林益元素生物科技有限公司 | A kind of method extracting Mengiferin from Folium mangiferae |
| CN104311614A (en) * | 2014-11-05 | 2015-01-28 | 桂林益元素生物科技有限公司 | Method for extracting mangiferin from mango leaves |
| CN108239079B (en) * | 2016-12-23 | 2021-07-06 | 中国医学科学院药物研究所 | Mangiferin crystal V-shaped substance, preparation method, composition and application thereof |
| CN108239079A (en) * | 2016-12-23 | 2018-07-03 | 中国医学科学院药物研究所 | Mangiferin V type substance of crystalline substance and preparation method and its composition and purposes |
| CN107048471A (en) * | 2017-05-08 | 2017-08-18 | 广西中烟工业有限责任公司 | A kind of preparation method of mango leaf extract and its application in cigarette |
| CN107383130A (en) * | 2017-07-20 | 2017-11-24 | 广西南宁桂知科技有限公司 | A kind of method that mangiferin is prepared using myrica rubra leaf |
| CN108872440A (en) * | 2018-08-11 | 2018-11-23 | 中山市永恒化工新材料研究院有限公司 | The measurement method of phenol content in a kind of water |
| CN108872440B (en) * | 2018-08-11 | 2021-06-29 | 中山市永恒化工新材料研究院有限公司 | Method for measuring content of phenol in water |
| CN109081859A (en) * | 2018-09-27 | 2018-12-25 | 那坡康正天然植物提取有限责任公司 | The production method of mangiferin |
| CN109776515A (en) * | 2019-03-29 | 2019-05-21 | 广西工业职业技术学院 | The method of mangiferin is extracted from myrica rubra leaf |
| CN109912582A (en) * | 2019-03-29 | 2019-06-21 | 广西工业职业技术学院 | The method of mangiferin is extracted from mango leaf |
| CN113416182A (en) * | 2021-05-28 | 2021-09-21 | 西安金泰生物技术有限公司 | Mangiferin and preparation method thereof |
| CN113416182B (en) * | 2021-05-28 | 2022-03-29 | 西安金泰生物技术有限公司 | Mangiferin and preparation method thereof |
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Application publication date: 20090513 |