CN104306414A - Glehniae radix extraction method - Google Patents
Glehniae radix extraction method Download PDFInfo
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- CN104306414A CN104306414A CN201410616997.3A CN201410616997A CN104306414A CN 104306414 A CN104306414 A CN 104306414A CN 201410616997 A CN201410616997 A CN 201410616997A CN 104306414 A CN104306414 A CN 104306414A
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- radix glehniae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a glehniae radix extraction method. The glehniae radix extraction method includes the following operation steps: (1) glehniae radix roots go through rinsing, sun-drying and smashing, and then undergo vacuum drying to achieve a balance weight; (2) the dried glehniae radix roots are placed in a Soxhlet extractor, extraction is carried out for 4 to 6 hours after cyclohexane is added, and the cyclohexane is volatilized; (3) deionized water is added, and heated digestion is carried out for 2 to 3 hours after the glehniae radix roots are soaked at the room temperature for 2 to 4 hours; (4) an extracting solution is then obtained through centrifugal separation, residues are squeezed within a pressure range of 25 to 30 MPS to obtain juice, the juice is combined with the extracting solution after juicing is completed, and the herb residues are reserved for later use; (5) the herb residues are taken out, water is added to wash the herb residues for multiple times, and an obtained washing solution is further combined with the extracting solution obtained through (4), so as to obtain a glehniae radix extracting solution; (6) the extracting solution obtained after the combination goes through vacuum concentration, absolute ethyl alcohol is added into a concentrated solution to obtain precipitates, and the precipitates are placed in a refrigerator and stand overnight; the precipitates go through suction filtration, sedimentation, double washing respectively and successively by 95 percent ethyl alcohol, absolute ethyl alcohol and acetone, and vacuum drying, so as to obtain glehniae radix polysaccharide.
Description
Technical field
The invention belongs to Separation of Natural Products purification art, specifically relate to the extracting method of a kind of Radix Glehniae.
Background technology
Radix Glehniae (Glehniae Radix) has another name called Glehnia littorlis, glehniae,radix, RADIX GLEHNIAE from Liaoning of China, is the dry root of samphire glehnia littoralis Glehnia littoralis Fr. Schmidt ex Miq..This moral character is sweet, mildly bitter flavor, is slightly cold, and attaches to the lung and stomach meridians, has effect of nourishing YIN and clearing away lung-heat, reinforcing stomach reg fluid, thirsty for lung-heat type cough, chronic cough expectorant blood, calentura Tianjin wound, is clinical conventional Chinese medicine.Except medicinal, can also be used for developing health food.Radix Glehniae is mainly containing Coumarins and carbohydrate chemistry composition, and other also have volatile oil, alkaloid, phospholipid, aminoacid, trace element etc., and the polysaccharide topmost active component that is it, can the immunologic function of enhancing body effectively.The traditional habitat processing method of Radix Glehniae is removing fibrous root, cleans, slightly dries in the air, and puts after scalding in boiling water, removing root bark.At present, Chinese scholars mainly concentrates on planting technology, physiologically active and chemical composition aspect to the correlational study of Radix Glehniae, reports less to the extracting and developing of its effective ingredient and the research of Optimization Technology; Patent No. CN200510056142.0 discloses a kind of preparation method of eliminating phlegm and stopping cough mongolian medicine, it is for subsequent use that its amount of Radix Glehniae first being taken out 20-40% is ground into 60 ~ 100 order fine powders, again all the other medical materials are added alcohol reflux twice, merge extracted twice liquid to filter, reclaim ethanol, be condensed into paste.It is the more general method that plant produced uses that above-mentioned circumfluence method is extracted, but its Extracting temperature is higher, and cause power consumption high, paste-forming rate is high, and the post-processed prepared to preparation makes troubles, and improves production cost.
Summary of the invention
Main purpose of the present invention is the extracting method providing a kind of Radix Glehniae, comparatively fully can not only extract the effective ingredient in Radix Glehniae, ensure that higher medical material utilization rate and extraction ratio, effectively can also reduce paste-forming rate, be convenient to later stage preparation.Extracting method of the present invention has the advantages such as Extracting temperature is low, solvent load is few, save a large amount of costs, being conducive to for producing
Energy-conserving and environment-protective.
In order to achieve the above object, the present invention adopts following technical scheme: the extracting method of a kind of Radix Glehniae, comprises following operating procedure:
(1) root of Radix Glehniae through rinsing, dry, pulverize after, drying under reduced pressure is to weight;
(2) dried Radix Glehniae is put into apparatus,Soxhlet's, add cyclohexane 4-6h, fling to cyclohexane extraction;
(3) deionized water is added, after soaking at room temperature 2-4h, heating lixiviate 2-3h;
(4) then centrifugally separate extracting solution, residue is squeezed extracting juice, and squeeze pressure is 25-30MPa, wait squeezing the juice completely, and merge extractive liquid, medicinal residues are for subsequent use;
(5) to get it filled slag, then add water washing repeatedly, after gained cleaning mixture and step (4) extracting solution merge further, obtain Radix Glehniae extracting solution; Wherein, the concrete operations of washing are as follows: the water getting former Radix Glehniae 2 times of weight, after mixing, presses down extracting juice at 25-30MPa, obtain cleaning mixture;
(6) by the extracting solution concentrating under reduced pressure after above-mentioned merging, in concentrated solution, add dehydrated alcohol, obtain precipitate, put into refrigerator hold over night; Sucking filtration, precipitates and respectively washs 2 times successively through 95% ethanol, dehydrated alcohol, acetone, and reduced vacuum is dry, obtains Polysaccharide.
As further preferred, in described step (3), heating-up temperature is 40-80 DEG C.
As further preferably, in described step (5), add water washing 3 times.
As further preferably, in concentrated solution, add dehydrated alcohol in described step (6) to containing alcohol 70%.
As further preferably, 60 DEG C of reduced vacuum dryings in described step (6).
The invention has the beneficial effects as follows: lixiviate and squeezing combine and extract by the present invention, comparatively fully can not only extract the effective ingredient in medical material, ensure that higher medical material utilization rate and extraction ratio, effectively can also reduce paste-forming rate, be convenient to later stage preparation.Extracting method of the present invention has the advantages such as Extracting temperature is low, solvent load is few, can be that large production saves a large amount of costs, be conducive to energy-conserving and environment-protective.
Detailed description of the invention
The realization of the object of the invention, functional characteristics and beneficial effect, be described further below in conjunction with specific embodiment.
Below in conjunction with specific embodiment, technical scheme of the present invention is described in further detail, can better understand the present invention to make those skilled in the art and can be implemented, but illustrated embodiment is not as a limitation of the invention.
Embodiment 1: the extracting method of the embodiment of the present invention 1 Radix Glehniae, comprises following operating procedure:
(1) root of Radix Glehniae through rinsing, dry, pulverize after, drying under reduced pressure is to weight;
(2) dried Radix Glehniae 10g is put into apparatus,Soxhlet's, add cyclohexane extraction 300ml and extract 4-6h, fling to cyclohexane extraction;
(3) add deionized water 200ml, after soaking at room temperature 3h, be heated to 60 DEG C of lixiviate 3h;
(4) then centrifugally separate extracting solution, residue is squeezed extracting juice, and squeeze pressure is 25MPa, wait squeezing the juice completely, and merge extractive liquid, medicinal residues are for subsequent use;
(5) to get it filled slag, then add water washing 3 times, after gained cleaning mixture and step (4) extracting solution merge further, obtain Radix Glehniae extracting solution; Wherein, the concrete operations of washing are as follows: the water getting former Radix Glehniae 2 times of weight, after mixing, presses down extracting juice at 25MPa, obtain cleaning mixture;
(6) extracting solution after above-mentioned merging is evaporated to 50ml, in concentrated solution, adds dehydrated alcohol to containing alcohol 70%, obtain precipitate polysaccharide, put into refrigerator hold over night; Sucking filtration, precipitate and respectively wash 2 times successively through 95% ethanol, dehydrated alcohol, acetone, 60 DEG C of reduced vacuum dryings, obtain Polysaccharide 40.0mg, are dissolved in water and are settled to 1000mL, for subsequent use.Gained total polysaccharides content is 35.46%, and paste-forming rate is 0.45g/g.
Comparative example: reflux, extract,
Poured in round-bottomed flask by dried 10g Radix Glehniae root herb powder and add 100mL water, backflow 2h, sucking filtration, obtains filtrate, is evaporated to 20 mL.In filtrate, add ethanol makes alcohol content reach 75%, and limit edged does not stop to stir, and hold over night is centrifugal, abandons supernatant, gets precipitation, dry, is dissolved in water and is settled to 50mL, for subsequent use.Calculating total polysaccharides content is 24.22%.
The assay of total polysaccharides in sample:
Accurate absorption each sample solution 1mL, be placed in 50mL volumetric flask, be diluted with water to scale, then draw 2mL and add 5% phenol 1mL, drip concentrated sulphuric acid 5mL rapidly, separately get 2mL distilled water as blank, add 5% phenol and concentrated sulphuric acid respectively according to above step, place 5min, heat 15min in 80 C water bath, to be cooled to room temperature, measure absorbance.According to following formulae discovery total polysaccharides content.
Total polysaccharides content (%)={ (sample concentration) × extension rate × sample solution volume/Radix Glehniae root meal quality } × 100%.
Claims (5)
1. an extracting method for Radix Glehniae, is characterized in that: comprise following operating procedure:
1) root of Radix Glehniae through rinsing, dry, pulverize after, drying under reduced pressure is to weight;
2) dried Radix Glehniae is put into apparatus,Soxhlet's, add cyclohexane 4-6h, fling to cyclohexane extraction;
3) deionized water is added, after soaking at room temperature 2-4h, heating lixiviate 2-3h;
4) then centrifugally separate extracting solution, residue is squeezed extracting juice, and squeeze pressure is 25-30MPa, wait squeezing the juice completely, and merge extractive liquid, medicinal residues are for subsequent use;
5) to get it filled slag, then add water washing repeatedly, after gained cleaning mixture and step (4) extracting solution merge further, obtain Radix Glehniae extracting solution; Wherein, the concrete operations of washing are as follows: the water getting former Radix Glehniae 2 times of weight, 6) mixing after, press down extracting juice at 25-30MPa, obtain cleaning mixture;
By the extracting solution concentrating under reduced pressure after above-mentioned merging, in concentrated solution, add dehydrated alcohol, obtain precipitate, put into refrigerator hold over night; Sucking filtration, precipitates and respectively washs 2 times successively through 95% ethanol, dehydrated alcohol, acetone, and reduced vacuum is dry, obtains Polysaccharide.
2. the extracting method of Radix Glehniae according to claim 1, is characterized in that: in described step (3), heating-up temperature is 40-80 DEG C.
3. the extracting method of Radix Glehniae according to claim 1, is characterized in that: add water washing 3 times in described step (5).
4. the extracting method of Radix Glehniae according to claim 1, is characterized in that: in concentrated solution, add dehydrated alcohol in described step (6) to containing alcohol 70%.
5. the extracting method of Radix Glehniae according to claim 1, is characterized in that: 60 DEG C of reduced vacuum dryings in described step (6).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410616997.3A CN104306414A (en) | 2014-11-06 | 2014-11-06 | Glehniae radix extraction method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410616997.3A CN104306414A (en) | 2014-11-06 | 2014-11-06 | Glehniae radix extraction method |
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| CN104306414A true CN104306414A (en) | 2015-01-28 |
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| CN201410616997.3A Pending CN104306414A (en) | 2014-11-06 | 2014-11-06 | Glehniae radix extraction method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859903A (en) * | 2016-04-29 | 2016-08-17 | 河北科技大学 | Radix glehniae polysaccharide and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2654106Y (en) * | 2003-09-16 | 2004-11-10 | 丁望 | Medical squeezer |
| CN103059080A (en) * | 2012-12-13 | 2013-04-24 | 大兴安岭林格贝有机食品有限责任公司 | Process for separating and purifying paeoniflorin |
| CN103705557A (en) * | 2013-11-13 | 2014-04-09 | 江苏省中国科学院植物研究所 | Radix glehniae root bark total polysaccharide and application thereof |
-
2014
- 2014-11-06 CN CN201410616997.3A patent/CN104306414A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2654106Y (en) * | 2003-09-16 | 2004-11-10 | 丁望 | Medical squeezer |
| CN103059080A (en) * | 2012-12-13 | 2013-04-24 | 大兴安岭林格贝有机食品有限责任公司 | Process for separating and purifying paeoniflorin |
| CN103705557A (en) * | 2013-11-13 | 2014-04-09 | 江苏省中国科学院植物研究所 | Radix glehniae root bark total polysaccharide and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 侯宗福等: "北沙参水溶性多糖提取工艺", 《南昌大学学报》 * |
| 刘波等: "采用两种方法加工的北沙参粗多糖的提取及对阴虚型小鼠的免疫调节作用比较", 《中华中医药学会四大怀药与地道药材研究论坛暨中药炮制分会第二届第五次学术会议与第三届会员代表大会论文集》 * |
| 李颖等: "北沙参多糖提取工艺的研究", 《食品科技》 * |
| 赵玉英: "《天然药物化学》", 31 March 2012, 北京大学医学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105859903A (en) * | 2016-04-29 | 2016-08-17 | 河北科技大学 | Radix glehniae polysaccharide and preparation method and application thereof |
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