CN104297518A - Simple microfluid flow velocity measuring device - Google Patents
Simple microfluid flow velocity measuring device Download PDFInfo
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- CN104297518A CN104297518A CN201410564859.5A CN201410564859A CN104297518A CN 104297518 A CN104297518 A CN 104297518A CN 201410564859 A CN201410564859 A CN 201410564859A CN 104297518 A CN104297518 A CN 104297518A
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Abstract
The invention relates to a simple microfluid flow velocity measuring device. The simple microfluid flow velocity measuring device is composed of a microflow chip and a bubble detection image collecting device, wherein the microflow chip is formed by a fluid flow velocity measuring channel, a bubble injection channel and a bubble generating channel. One end of the bubble generating channel is sealed, and the other end of the bubble generating channel is communicated with the fluid flow velocity measuring channel. One end of the bubble injection channel is sealed. The sealed end of the bubble injection channel is opposite to the sealed end of the bubble generating channel. A breathable waterproof membrane is arranged between the two sealed ends, and the bubble detection image collecting device is externally connected with a computer. The simple microfluid flow velocity measuring device has the advantages that the flow velocity measuring mode is simple, fluid cannot flow back into a gas channel, gas is separated from the fluid after an experiment, and direct measurement of the flow velocity of the fluid is achieved. The simple microfluid flow velocity measuring device is reasonable in design and simple in structure, and is highly integrated, the fluid cannot be polluted, and the simple microfluid flow velocity measuring device is suitable for measurement experiments, needing to measure the microfluid flow velocity losslessly, of micro-fluidic chips.
Description
Technical field
The present invention relates to a kind of simple and easy microfluid flow rate measuring device.Belong to fluid measurement technology.
Background technology
The beginning of the nineties in last century micro-total analysis system put forward, and can realize the chip system that miniature biochemical is analyzed.Micro-total analysis system has the distinguishing features such as detection speed is fast, style consumption is few, flux is high.Dimethyl silicone polymer (PDMS), as a kind of polymkeric substance having the fabulous transparency, low cytotoxicity, gas penetration potential, has been widely used in biochemical micro-total analysis system with the micro-fluidic chip that PDMS and slide are base material.In micro flow chip system, working fluid is main bearing medium, carries out actual flow velocity measurement, and regulate and control according to actual needs microfluid, has been one of biochemical analysis checkout procedure core parameter, is also one of micro-fluidic core technology.The flow velocity of microfluid is less, cannot accurately measure its flow velocity by the fluid velocity measuring method of routine.
Have at rate of flow of fluid measurement mechanism at present: ultrasound speed variator, as (patent CN201310001989), photoelectricity voltage fluctuation signal speed measuring device, as (patent CN200910103372), or spike particulate imaging speed measuring device, as devices such as (patents 201010163676) measures the flow velocity of microfluid.But mode brings many problems above, such as introduce other probe materials, cannot Measurement accuracy and processed complex etc.Common fluids fluid-velocity survey can solve contaminant problem by reducing precision, or introducing trace impurity improves precision.But, ensure that the microfluid fluid-velocity survey of precision and degree of purity is but controling parameters necessary in micro-total analysis chip system simultaneously.Meanwhile, introducing impurity is that major part experiment does not allow occurrence, introduces impurity or measuring error easily causes the failure of an experiment in degree of purity and the higher experiment of accuracy requirement.Therefore, microfluid fluid-velocity survey problem has become the long-term major obstacle hindering micro-total analysis system development.
Summary of the invention
The object of the invention is to overcome the defect that above-mentioned prior art exists, provide a kind of structure simply, the simple and easy microfluid flow rate measuring device of safety and sanitation again.
To achieve these goals, technical scheme of the present invention:
Simple and easy microfluid flow rate measuring device, described measurement mechanism to be tested the speed passage by rate of flow of fluid, gas inject passage, the micro flow chip that bubble formation passage is formed and bubble detected image harvester composition; Bubble formation passage end is closed, the other end and the rate of flow of fluid passage that tests the speed is interconnected, one end, bubble injection channel is closed, the blind end of bubble injection channel and the blind end of bubble formation passage positioned opposite, and be provided with breathing waterproof film between two blind ends, the external computing machine of bubble detected image harvester.
Described air permeable waterproof film thickness is 1 μm-1000 μm.
The described rate of flow of fluid passage that tests the speed adopts transparent watertight material.
Rate of flow of fluid tests the speed passage, and the width of gas inject passage and bubble formation passage is 10 μm-1000 μm.
Owing to have employed above technical scheme, microfluid flow rate measuring device provided by the invention possesses following beneficial effect:
1) the present invention only needs three passages just can complete fluid-velocity survey chip, and apparatus structure is simple.
2) in spike bubble injection process owing to adopting special breathing waterproof film to intercept gas inject raceway groove and bubble formation raceway groove, therefore there will not be liquid to flow back to the phenomenon of gas inject raceway groove.
3) because gas is not direct and fluid-velocity survey passage is come round, therefore can not form gas column on flow velocity tests the speed raceway groove affects fluid-velocity survey.
4) probe material of fluid-velocity survey adopts the gas do not reacted with fluid to be measured, and is discharged by breathing waterproof film after having tested the speed, therefore on the fluid in microchannel without impact, do not introduce any impurity, safety and sanitation.
5) rate of flow of fluid measurement has been come by spike bubble, and be therefore the direct measurement of the flow velocity of convection cell, measuring accuracy is high.
The present invention is reasonable in design, and structure is simple, high integration, and convection cell can not cause any pollution, and being applicable to needs nondestructive measurement microfluid fluid-velocity survey to test in micro-fluidic chip.
Accompanying drawing explanation
Fig. 1 is the structural representation that the invention provides device.
Fig. 2 is the vertical view of Fig. 1 chips 2.
Embodiment
Below in conjunction with the using method of accompanying drawing and device, the invention will be further described.
See accompanying drawing
Simple and easy microfluid flow rate measuring device, described measurement mechanism to be tested the speed passage 23 by rate of flow of fluid, gas inject passage 21, and the micro flow chip 2 that bubble formation passage 22 is formed and bubble detected image harvester 1 form, described rate of flow of fluid tests the speed passage 23, the width of gas inject passage 21 and bubble formation passage 22 is 10 μm-1000 μm, different width is adopted for different fluids, the described rate of flow of fluid passage 23 that tests the speed adopts transparent watertight material, the end of described gas inject interface is provided with the air valve for controlling gas flow, described bubble formation passage 22 one end is closed, the other end and the rate of flow of fluid passage 23 that tests the speed is interconnected, one end, bubble injection channel 21 is closed, the blind end of bubble injection channel 21 and the blind end of bubble formation passage 22 positioned opposite, and be provided with breathing waterproof film 31 between two blind ends, described air permeable waterproof film thickness is 1 μm-1000 μm, different thickness is adopted for different gas breathing waterproof films.Described bubble detected image harvester 1 is CCD camera, and this camera is fixed on above fluid-velocity survey passage by support, by the external computing machine of USB interface.
The using method of this device is:
(1) as shown in Figure 1, the chip made is placed on immediately below CCD camera 1 camera lens;
(2) as shown in Figure 2, by microchannel, fluid is injected raceway groove from fluid-infusing port 12;
(3) as shown in Figure 2, after microfluid velocity-stabilization, inject from inert gas inlet 11 gas do not reacted with testing liquid;
(4) control CCD camera with certain position picture taking bubble across frame time t continuously, obtain the trajectory diagram of bubble.
(5) by shooting to bubble track picture pass to host computer and process, utilize the relative position of adjacent picture bubble
with
with the flow velocity V=(S calculating microfluid across frame time t
n-S
n-1) ÷ t.
(6) after having measured, micro flow chip is saved, continue to use when next time measures microfluid of the same race.
Visible, the present invention is applicable to the fluid-velocity survey of various very low volume fluids, such as, flow speed real-time measurement etc. at the control of micro flow chip liquid, complex fluid (blood).Building of microfluid flow rate measuring device of the present invention is very simply and easily process.In the cost of device, owing to measuring embedding raceway groove not having hardware, greatly save the cost of measurement, for when requiring higher microfluid fluid-velocity survey, this chip can accomplish single use, and the present invention is at the technically real nondestructive measurement accomplished liquid of micro-fluid measurement.
Claims (4)
1. simple and easy microfluid flow rate measuring device, it is characterized in that: described measurement mechanism to be tested the speed passage (23) by rate of flow of fluid, gas inject passage (21), the micro flow chip (2) that bubble formation passage (22) is formed and bubble detected image harvester (1) composition; Bubble formation passage (22) one end is closed, the other end and the rate of flow of fluid passage (23) that tests the speed is interconnected, bubble injection channel (21) one end is closed, the blind end of bubble injection channel (21) and the blind end of bubble formation passage (22) positioned opposite, and be provided with breathing waterproof film (31) between two blind ends, bubble detected image harvester (1) external computing machine.
2. simple and easy microfluid flow rate measuring device as claimed in claim 1, is characterized in that: described air permeable waterproof film thickness is 1 μm-1000 μm.
3. simple and easy microfluid flow rate measuring device as claimed in claim 1, is characterized in that: the described rate of flow of fluid passage (23) that tests the speed adopts transparent watertight material.
4. simple and easy microfluid flow rate measuring device as claimed in claim 1, is characterized in that: rate of flow of fluid tests the speed passage (23), and the width of gas inject passage (21) and bubble formation passage (22) is 10 μm-1000 μm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410564859.5A CN104297518B (en) | 2014-10-22 | 2014-10-22 | Simple microfluid flow velocity measuring device |
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| CN201410564859.5A CN104297518B (en) | 2014-10-22 | 2014-10-22 | Simple microfluid flow velocity measuring device |
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| CN104297518A true CN104297518A (en) | 2015-01-21 |
| CN104297518B CN104297518B (en) | 2017-01-25 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104820112A (en) * | 2015-04-24 | 2015-08-05 | 华南理工大学 | Plant leaf vein flow velocity measurement device and method |
| CN109738113A (en) * | 2018-12-25 | 2019-05-10 | 西安交通大学 | Pressure test method in a kind of microchannel based on microbubble |
Citations (8)
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| US4316391A (en) * | 1979-11-13 | 1982-02-23 | Ultra Med, Inc. | Flow rate measurement |
| CN101498739A (en) * | 2009-03-13 | 2009-08-05 | 重庆交通大学 | Optoelectronic contact type flow-speed measurement method and sensor thereof |
| CN101852814A (en) * | 2010-04-29 | 2010-10-06 | 中国农业大学 | Whole-field testing method for internal flow of drip emitter maze flow channel |
| US20120140240A1 (en) * | 2009-12-04 | 2012-06-07 | The Trustees Of Columbia University In The City Of New York | Laser-scanning intersecting plane tomography such as for high speed volumetric optical imaging |
| CN102901830A (en) * | 2012-10-15 | 2013-01-30 | 浙江大学 | Construction method for high-throughput micro-fluidic chip detecting system |
| CN103063868A (en) * | 2013-01-05 | 2013-04-24 | 浙江大学 | Device and method for measuring flow rate of fluid |
| CN103185808A (en) * | 2012-03-30 | 2013-07-03 | 嘉兴学院 | Photoelectric technology-based bubble velocity-measuring system |
| CN204086296U (en) * | 2014-10-22 | 2015-01-07 | 武汉纺织大学 | Simple and easy microfluid flow rate measuring device |
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2014
- 2014-10-22 CN CN201410564859.5A patent/CN104297518B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4316391A (en) * | 1979-11-13 | 1982-02-23 | Ultra Med, Inc. | Flow rate measurement |
| CN101498739A (en) * | 2009-03-13 | 2009-08-05 | 重庆交通大学 | Optoelectronic contact type flow-speed measurement method and sensor thereof |
| US20120140240A1 (en) * | 2009-12-04 | 2012-06-07 | The Trustees Of Columbia University In The City Of New York | Laser-scanning intersecting plane tomography such as for high speed volumetric optical imaging |
| CN101852814A (en) * | 2010-04-29 | 2010-10-06 | 中国农业大学 | Whole-field testing method for internal flow of drip emitter maze flow channel |
| CN103185808A (en) * | 2012-03-30 | 2013-07-03 | 嘉兴学院 | Photoelectric technology-based bubble velocity-measuring system |
| CN102901830A (en) * | 2012-10-15 | 2013-01-30 | 浙江大学 | Construction method for high-throughput micro-fluidic chip detecting system |
| CN103063868A (en) * | 2013-01-05 | 2013-04-24 | 浙江大学 | Device and method for measuring flow rate of fluid |
| CN204086296U (en) * | 2014-10-22 | 2015-01-07 | 武汉纺织大学 | Simple and easy microfluid flow rate measuring device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104820112A (en) * | 2015-04-24 | 2015-08-05 | 华南理工大学 | Plant leaf vein flow velocity measurement device and method |
| CN104820112B (en) * | 2015-04-24 | 2018-01-05 | 华南理工大学 | A kind of device and method of plant leaf vein flow velocity measurement |
| CN109738113A (en) * | 2018-12-25 | 2019-05-10 | 西安交通大学 | Pressure test method in a kind of microchannel based on microbubble |
Also Published As
| Publication number | Publication date |
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| CN104297518B (en) | 2017-01-25 |
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