CN104297327A - 一种采用微流控芯片分析血清脂蛋白亚型精细亚组分的方法 - Google Patents
一种采用微流控芯片分析血清脂蛋白亚型精细亚组分的方法 Download PDFInfo
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- CN104297327A CN104297327A CN201410590606.5A CN201410590606A CN104297327A CN 104297327 A CN104297327 A CN 104297327A CN 201410590606 A CN201410590606 A CN 201410590606A CN 104297327 A CN104297327 A CN 104297327A
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CN201410590606.5A CN104297327B (zh) | 2014-10-29 | 2014-10-29 | 一种采用微流控芯片分析血清脂蛋白亚型精细亚组分的方法 |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104316710A (zh) * | 2014-10-29 | 2015-01-28 | 邓杏飞 | 一种血清脂蛋白亚型精细亚组分微流控芯片分析系统 |
WO2017020830A1 (zh) * | 2015-08-03 | 2017-02-09 | 复旦大学附属中山医院 | 改良凝胶电泳检测血清前β1高密度脂蛋白的方法和应用 |
CN113740278A (zh) * | 2021-03-10 | 2021-12-03 | 谱天(天津)生物科技有限公司 | 一种测定极低密度脂蛋白亚组份成分分布的方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1168720A (zh) * | 1994-08-01 | 1997-12-24 | 罗克贺德马丁能源系统有限公司 | 对化学分析和合成进行微流体处理的装置和方法 |
WO2001069226A1 (en) * | 2000-03-10 | 2001-09-20 | Dna Sciences, Inc. | Cross channel device for serial sample injection |
CN1740779A (zh) * | 2005-09-22 | 2006-03-01 | 复旦大学 | 一种基于集成化微流控芯片的分析系统和分析方法 |
CN101692047A (zh) * | 2009-10-27 | 2010-04-07 | 浙江大学 | 毛细管电泳分离和化学发光检测的微流控芯片 |
CN102788831A (zh) * | 2012-08-13 | 2012-11-21 | 中国科学院研究生院 | 分离后可调节pH的微流控芯片电泳-电化学检测装置及其应用 |
CN103344631A (zh) * | 2013-07-15 | 2013-10-09 | 浙江省海洋水产研究所 | 非水电泳分离和过氧草酸酯类化学发光检测的微流控芯片 |
-
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Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1168720A (zh) * | 1994-08-01 | 1997-12-24 | 罗克贺德马丁能源系统有限公司 | 对化学分析和合成进行微流体处理的装置和方法 |
WO2001069226A1 (en) * | 2000-03-10 | 2001-09-20 | Dna Sciences, Inc. | Cross channel device for serial sample injection |
CN1740779A (zh) * | 2005-09-22 | 2006-03-01 | 复旦大学 | 一种基于集成化微流控芯片的分析系统和分析方法 |
CN101692047A (zh) * | 2009-10-27 | 2010-04-07 | 浙江大学 | 毛细管电泳分离和化学发光检测的微流控芯片 |
CN102788831A (zh) * | 2012-08-13 | 2012-11-21 | 中国科学院研究生院 | 分离后可调节pH的微流控芯片电泳-电化学检测装置及其应用 |
CN103344631A (zh) * | 2013-07-15 | 2013-10-09 | 浙江省海洋水产研究所 | 非水电泳分离和过氧草酸酯类化学发光检测的微流控芯片 |
Non-Patent Citations (4)
Title |
---|
BRUCE H. WEILLER ET AL.: "Analysis of Lipoproteins by Capillary Zone Electrophoresis in Microfluidic Devices: Assay Development and Surface Roughness Measurements", 《ANAL. CHEM.》 * |
GUICHEN PING ET AL.: "Analysis of Lipoproteins by Microchip Electrophoresis with High Speed and High Reproducibility", 《ANAL. CHEM.》 * |
HUA WANG ET AL.: "Simultaneous Determination of High-Density Lipoprotein,Very Low-Density Lipoprotein and Low-Density Lipoprotein Subclass in Human Serum by Microchip CE", 《CHROMATOGRAPHIA》 * |
汪骅等: "基于纳米技术的芯片电泳快速检测脂蛋白及其亚型的研究", 《检验医学》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104316710A (zh) * | 2014-10-29 | 2015-01-28 | 邓杏飞 | 一种血清脂蛋白亚型精细亚组分微流控芯片分析系统 |
WO2017020830A1 (zh) * | 2015-08-03 | 2017-02-09 | 复旦大学附属中山医院 | 改良凝胶电泳检测血清前β1高密度脂蛋白的方法和应用 |
CN113740278A (zh) * | 2021-03-10 | 2021-12-03 | 谱天(天津)生物科技有限公司 | 一种测定极低密度脂蛋白亚组份成分分布的方法 |
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Effective date of registration: 20161207 Address after: Floor 11, building C1, No. fourth, Kaiyuan Avenue, Science City, Guangzhou hi tech Industrial Development Zone, Guangdong, China Applicant after: Guangdong Guosheng medical Polytron Technologies Inc Address before: The United States of California mountain view showers Lane No. 49 T409 unit Applicant before: Deng Xingfei |
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Address after: 510730 Guangdong Guangzhou high tech Industrial Development Zone, Science City, open source road 11, C1 fourth. Patentee after: Guangdong Guosheng Medical Technology Co Ltd Address before: 510000 Guangdong Guangzhou high tech Industrial Development Zone, Science City, open source road 11, C1 fourth. Patentee before: Guangdong Guosheng medical Polytron Technologies Inc |
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Denomination of invention: A method for analyzing fine sub components of serum lipoprotein subtypes by microfluidic chip Effective date of registration: 20211214 Granted publication date: 20170118 Pledgee: Bank of China Limited by Share Ltd. Guangzhou Panyu branch Pledgor: GUANGDONG ARDENT BIOMED TECHNOLOGY CO.,LTD. Registration number: Y2021980014989 |