CN104287861A

CN104287861A - Mouse spinal cord contusion modeling experiment table

Info

Publication number: CN104287861A
Application number: CN201410607030.9A
Authority: CN
Inventors: 李英; 程建青; 孙荣棋; 朱沛姚
Original assignee: Jiangnan University
Current assignee: Jiangnan University
Priority date: 2014-10-31
Filing date: 2014-10-31
Publication date: 2015-01-21

Abstract

The invention provides a mouse spinal cord contusion modeling experiment table. A magnetic bead falling rack is placed on a mouse fixing platen, and magnets attract magnetic beads to fall to hit and damage the mouse spinal cord. The mouse fixing platen is a rectangular metal baking finish plate surface being 24 cm in length and 16 cm in width, a four-leg support is arranged below the mouse fixing platen, a graduated scale of 0.5 cm in unit is placed on the long edges and the center horizontal axis of the plate surface respectively, four mouse limb fixing knobs are arranged on the plate surface in a front and back manner, a tooth fixing hook is arranged in the center, 2 cm away from the front edge, of the plate surface, magnet plates of different specifications are arranged at the bottom of the platen, the magnetic bead falling rack is a circling tetrahedron made of synthetic glass, the front side and the rear side of the magnetic bead falling rack are open, the magnetic bead falling rack is 20 cm in height, 10 cm in length in the front-and-back direction and 12 cm in width in the left-and-right direction, a cylindrical magnetic bead slide-down pipe with the internal diameter of 8 cm is arranged on the axis of each of the upper side and the lower side of the magnetic bead falling rack, and a height adjustment knob is arranged in the middle of each of the left side and the right side of the bottom of the magnetic bead falling rack to adjust the bottom height of the magnetic bead falling rack.

Description

A kind of mouse spinal cord dampens molding laboratory table

Technical field:

Medical teaching, scientific experiment mini-plant

Background technology:

Spinal cord injury is mainly among major motor vehicle and the natural disaster such as work accident and earthquake, after spinal cord injury, occurs various motion, sensation and sphincter dysfunction, the phenomenon of the corresponding change such as dystonia and pathologic reflex at the corresponding sections of infringement.Model is dampened or weighed wounded to passivity close to the injured reality of human myeloid, is the good model of research spinal cord injury.Within 1911, Allen utilizes weight method of falling first to make spinal cord contusion model, and the method was called as Allen punch method afterwards.Its principle clashes into spinal cord for utilizing mechanical force, makes spinal cord under stress, produce elasticity and becomes and cause injury.Method, for exposing dorsal part spinal cord, retains spinal dura mater, freely falls with standard quality weight along hollow conduit, and spinal cord injury energy adopts weight quality (gm) to weigh with the product of height of drop (cm).The advantage of this model is that cost is low, easy to operate, and lesion level is clear and definite, after damage pathological change and dysfunction more typical, degree of injury scalable, light, severe injury favorable repeatability, infection chance is less.The method shortcoming model influence factor that is to cause injury is many, need excise vertebral plate, easy damaged spinal cord blood vessel and spinal nerves; Impact site is not easily accurately determined, compression of spinal cord moment pressure change comparability is poor; Weight bounce-back can make spinal cord repeatedly be hit, moderate lesion repeatability difference etc.

Summary of the invention:

For spinal cord passivity dampen or weigh wounded experimental model exist the model influence factor that causes injury many, the problems such as vertebral plate, easy damaged spinal cord blood vessel and spinal nerves need be excised, the invention provides a kind of mouse spinal cord and dampen molding laboratory table, pass through magnetic field intensity, control degrees of spinal cord injury, impact site is accurate, does not need to excise vertebral plate.

The technical solution adopted for the present invention to solve the technical problems is: on mice fixed station, place a magnetic bead whereabouts frame, the gravitation utilizing Magnet to fall to magnetic bead, wounds mouse spinal cord.Mice stationary platen is long 24 ㎝, wide 16 ㎝ rectangle metal baking finish plate faces, four leg supports are had under plate, there is gum cover in its foot, there is the graduated scale of 0.5 ㎝ unit at plate face length limit and transverse axis place, center, for mice Position Scale, forward and backward in plate face have four mice extremity fixed knobs, fix mice upper limb and lower limb use respectively, by after the fixing mice extremity of cotton rope knotting during experiment, the other end is directly fixed by knob, and there is a tooth fixation hook center, plate face apart from leading edge 2 ㎝ place, by line cover, fixing mice tooth.Place the magnet plate of different size in the bottom surface of stationary platen, form the magnetic field of varying strength.Magnetic bead whereabouts frame, forms around tetrahedron by lucite, and its forward and backward is open type, reference is done in the orientation of placing mice with fixed station, high 20 ㎝, fore-and-aft direction is length 10 ㎝, and left and right directions is width 12 ㎝, have the cylindrical magnetic bead of internal diameter 8 ㎜ to glide at the middle shaft part of above and below to manage, two-port flushes respectively and is embedded in above and below plate face, and during experiment, magnetic bead is slided by upper port, by magnetic field intensity, control degrees of spinal cord injury.In frame bottom surface, magnetic bead whereabouts, left and right sides pars intermedia has altitude mixture control knob, and the spacing between frame bottom surface, scalable magnetic bead whereabouts and mice stationary platen face, determines the distance relation between this bottom surface and the mice back side.

The final beneficial effect of this product is: after mice is fixing, impact site is accurate, and by magnetic field intensity, control degrees of spinal cord injury, can get rid of more influence factor, experimental implementation is simple and easy to do.

Accompanying drawing illustrates:

Below in conjunction with accompanying drawing, the present invention is further described.

Fig. 1 is mice fixed station ideograph.

Fig. 2 is mice fixed mesa graduated scale schematic diagram.

Fig. 3 is magnetic bead whereabouts frame ideograph.

Fig. 4 is magnetic bead whereabouts frame antapical view schematic diagram.

Fig. 5 is mouse spinal cord damage molding laboratory table Overall View ideograph.

1. mice stationary platens in figure, 2. fixed station four leg support, 3. tooth fixation hook, 4. mice extremity fixed knob, 5. there is gum cover in mice fixed station foot, 6. mice fixed mesa scale, 7. slip pipe under magnetic bead, 8. slip pipe upper port under magnetic bead, 9. magnetic bead downslide infratubal port, 10. altitude mixture control knob.

Detailed description of the invention:

By mouse anesthesia, back is upwards placed in (1) on mice stationary platen, the labial teeth of rat is enclosed within one end of cotton rope cover, the other end is enclosed within the tooth fixation hook (3) of operating board front end, after the fixing rat extremity of cotton rope knotting, the cotton rope other end is directly by four knob (4) fixing limbs, frame reference plate face scale (6) that fallen by magnetic bead is placed in mice stationary platen, aiming at mice spinal column with magnetic bead downslide infratubal port (9) will injury region, height is reserved with altitude mixture control knob (10), frame bottom surface, magnetic bead whereabouts is made to adjoin mouse back skin, selected magnet plate is placed in mice stationary platen bottom surface, magnetic bead is put into slip pipe (7) magnetic bead from upper port (8), magnetic bead slides, to cause injury mouse spinal cord.

Claims

1. mouse spinal cord dampens a molding laboratory table, and be configured to by a mice fixed station and magnetic bead whereabouts, it is characterized in that placing magnet plate bottom mice fixed station, the gravitation utilizing Magnet to fall to magnetic bead, wounds mouse spinal cord.

2. the mouse spinal cord according to claims 1 dampens molding laboratory table, it is characterized in that there is the graduated scale of 0.5 ㎝ unit at mice stationary platen face length limit and transverse axis place, center, and having four mice extremity fixed knobs, there is a tooth fixation hook center, plate face apart from leading edge 2 ㎝ place.

3. the mouse spinal cord according to claims 1 dampens molding laboratory table, and it is characterized in that magnetic bead whereabouts frame forms around tetrahedron by lucite, its forward and backward is open type.

4. the mouse spinal cord according to claims 1 dampens molding laboratory table, and it is characterized in that magnetic bead whereabouts frame has the cylindrical magnetic bead of internal diameter 8 ㎜ to glide at the middle shaft part of above and below and manages, two-port flushes respectively and is embedded in above and below plate face.

5. the mouse spinal cord according to claims 1 dampens molding laboratory table, it is characterized in that there is altitude mixture control knob at frame bottom surface, magnetic bead whereabouts left and right sides pars intermedia.