CN104286728A - Zinc-rich kidney bean product and production method thereof - Google Patents
Zinc-rich kidney bean product and production method thereof Download PDFInfo
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Abstract
The invention discloses a zinc-rich kidney bean product and a production method thereof. The production method is realized mainly by the processes of material preparation, sterilization and germination, and comprises the steps of soaking sterilized kidney beans in a culture solution prepared by 5-60mmol/ L of zinc sulfate and 10-60 micromol/ L of gibberellin at the temperature of 18-32 DEG C, and germinating to obtain the zinc-rich kidney bean product. After the kidney beans are treated by the method, the content of Zn in the kidney beans can be increased to 2987.44mg/Kg from 34.56mg/Kg; the zinc-rich content of the kidney beans treated by the method is 46.9% higher than that of the kidney beans treated by the prior art; the content of free amino acid is increased by 35.3%, the content of soluble protein is increased by 86.3%, and the content of polypeptide is increased by 15.6%; furthermore, the kidney beans only need to be treated for the shortest treatment time of 2 days, the treatment time is greatly shortened, and particularly, the possibility of destroying the germination process caused by the breeding of mould and bacteria in the treatment process can be effectively reduced; compared with the kidney beans which are purely treated by the zinc sulfate, the content of free amino acid, the content of soluble protein and the content of polypeptide of the kidney beans treated by the method are respectively increased by 52.9%, 77.0% and 16%.
Description
Technical field
The present invention relates to technical field of food production, particularly relate to a kind of rich zinc kidney bean goods and production method thereof.
Background technology
Zinc is requisite material in the important physiology courses such as growth in humans grows, reproduction heredity, immunity, endocrine, when human body reduces containing zinc total amount, immuning tissue can be caused impaired, immunodeficiency, so zinc is described as " flowers of life " by people.
The physiological action of zinc is mainly following aspect: promote growing and regeneration of body, zinc is that regulatory gene expresses the required part of archaeal dna polymerase namely regulating DNA replication dna, translate and transcribe, therefore, the outstanding symptom lacking zinc animal is that obstacle occurs for growth, protein synthesis, DNA and RNA metabolism etc.At human body, the growth lacking zinc children occurs to and has a strong impact on and occur iranian villager disease disease.No matter women or children lack the organization healing difficulty that zinc can make wound.Zinc is not only all required for the synthesis of protein and nucleic acid but also each process for the growth of cell, division and differentiation.Zinc contributes to the healing accelerating inside of human body and external wounds, eliminate the white dot on nail, prevent ageusia, contribute to the treatment to dysgenesis, contribute to preventing prostatic disorders, enhancing development and make resourceful, reduces the accumulation of cholesterol, contributes to treatment insane.Zinc can promote children ' s intelligence development.ZD children ' s intelligence development is bad, and the children that in body, Zn content is relatively high, then intelligence better and make a good academic record, so zinc has the effect promoting intelligence development.Therefore, scarce zinc be cause that bone in children poor growth, bone calcification are bad, appetite is low, digestive function reduces and the late-blooming major reason of entirety.Zinc is one of trace element important contained by human body, participates in composition and the activity of zinc enzyme and zinc dependent enzyme in body, plays an important role in tissue respiration and protein, fat, sugar and nucleic acid metabolism; Promote cell division growth and regeneration, therefore scarce zinc can affect Physique growth, intelligence development and reproductive function.
In addition, gustin contains zinc atom can maintain the sense of taste, promotes appetite; The right vision system of zinc deficiency has bad impact, causes amblygeustia.Zinc can formation is a kind of to play a driving role to the sense of taste and appetite containing zinc albumen-sialoprotein by participating in.Zinc affects retina retinol reductase activity, and participates in the synthesis of retinene, can affect when lacking zinc.When animal and human lacks zinc, there is anorexia.When oral histidine lacks zinc with the work that makes children (histidine can capture Binding in vivo in albuminous zinc, makes it to discharge from urine, causes in body and lacks zinc), appetite also can be caused significantly to go down.This all proves that zinc maintains the effect in dysregulated appetite.
Chinese patent CN200810150129.5, CN200810045505.4, CN88100006.X describe the preparation method of several zinc-rich bean sprouts respectively.Described in CN200810150129.5, Zn content in moyashi and mung bean sprouts is brought up to 3.8mg/100g(bean sprouts respectively) and 2.2mg/100g(bean sprouts), improve 7.03 times and 11 times; Organic zinc content in mung bean sprouts can be brought up to 104mg/Kg by its method described in CN200810045505.4; Described in CN88100006.X, its method can obtain the bean sprouts of 2032mg/kg Zn content; About 4 days germination institute's used times when said method reaches best enriching quantity.However, there is the defect of closing production cycle longer (generally all more than sky) and collection zinc amount not high (improving about 11 times) in above-mentioned method.
Summary of the invention
The object of this invention is to provide a kind of with short production cycle, rich zinc kidney bean goods that collection zinc amount is high and production method thereof.
The object of the invention is by following process implementation.
Get the raw materials ready: select even, crack-free, pucker-free kidney bean.
Sterilizing: soak sterilizing 20 ~ 40 minutes with 0.05 ~ 0.5% liquor natrii hypochloritis, liquor natrii hypochloritis's consumption was not to have kidney bean as well, clean with tap water rinse afterwards.
Preferably within every 8 ~ 12 minutes, shake once during soaking sterilizing, described rinsing preferably carries out 3 ~ 6 times.
Germinate: be mixed with mixed solution as nutrient solution by zinc sulfate 5 ~ 60mmol/L and gibberellin 10 ~ 60 μm of ol/L, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 6 ~ 24h is soaked at 15 ~ 35 DEG C, place 2 ~ 10 hours after elimination solution, repeat said process 2 ~ 6 times, namely again join in corresponding nutrient solution and soak 6 ~ 24h, place 2 ~ 10 hours after elimination solution, obtain rich zinc germination kidney bean.
As preferred technical scheme, above-mentioned germination temperature is 18 ~ 32 DEG C, the best is that 22 ~ 30 DEG C of described nutrient solutions are preferably zinc sulfate 10 ~ 40mmol/L and gibberellin 10 ~ 45 μm of ol/L, and the best is zinc sulfate 15 ~ 20mmol/L and gibberellin 15 ~ 25 μm of ol/L.
Above-mentioned medium pH value is preferably 5.8 ~ 6.2, regulates preferably by adding hydrochloric acid.
Further, after the rich zinc germination kidney bean drying of above-mentioned gained, powder can be ground into, as the raw material manufacturing kidney bean food further.
By process of the present invention, after testing, in kidney bean, Zn content rises to 2987.44mg/Kg from 34.56 mg/Kg, to 46.9% be exceeded than the rich Zn content after prior art process, free aminoacid content then increases by 35.3%, soluble protein content increases by 86.3%, content of peptides increases by 15.6%, and SPT only needs 2 day time, significantly shorten the processing time, particularly effectively reduce in processing procedure and mould and bacterial reproduction occur and the possibility destroying germination process, simultaneously, because gibberellin and zinc sulfate are to raising free aminoacid content, soluble protein content, there is synergy in content of peptides, make germination kidney bean Determination of Free Amino Acids, soluble protein content, content of peptides, 52.9% is improved respectively than the simple zinc sulfate that uses, 77.0%, 16%.
Accompanying drawing explanation
fig. 1for the change of 16 kinds of constituent contents in the kidney bean after actual measurement different disposal.
Fig. 2 is that 16 kinds of constituent contents of running water germination treatment and untreated samples contrast.
Germination kidney bean and untreated samples 15 kinds of constituent contents of Fig. 3 zinc sulfate and gibberellin process contrast.
Fig. 4 is kidney bean control sample infrared spectrogram.
Kidney bean infrared spectrogram after Fig. 5 zinc salt Stress treatment germinates.
Fig. 6 kidney bean infrared spectrum.
Fig. 7 kidney bean zinc salt Stress treatment and kidney bean control sample infrared spectrum.
Adopt ICP-OES to measure the trace element in kidney bean, its result
From measurement result, containing more mineral matter in Phaseolus vulgarisl, wherein the content of K, Mg, Ca, P is very high, with the content of these 4 kinds of elements in navy bean, there is identical trend, but also being rich in maintenance human normal vital movement required Zn, Fe, Cu, Mn, the content of Mo, Ni, Sr, Ba is relatively low, in addition, Al content is 5.06mg/kg, far below the standard of 100mg/kg in country " pollutants in food limitation " (GB 2762-2005).Therefore, Phaseolus vulgarisl can be developed to the excellent dietary product being rich in mineral element.
In test, Phaseolus vulgarisl is divided into three groups, process immersion with running water, ZnSO47H2O and GA3 respectively, in placement constant incubator 30 DEG C, cultivation 72h.Get three groups of kidney beans of germination and the bean sprouts of non-germination treatment and embryo totally four groups pull an oar, be ground to fine powder preservation with agate mortar after freeze-drying.Take 1.000g(and be accurate to 0.0001g) Phaseolus vulgarisl powder in 125mL beaker in tall form, add 15mL red fuming nitric acid (RFNA) and cover surface plate, soaked overnight.Place heating and decompose on electric hot plate, temperature controls in (200 ± 1) DEG C.Near dry to emitting white cigarette to continue heating, take off, add chloroazotic acid (1+1) 5mL after slightly cold, place on electric hot plate and continue heating, boil about about 10min to clarification, take off and be cooled to room temperature, be settled in 25mL colorimetric cylinder with ultra-pure water, dilute 2 times respectively, 10 times to be measured.Do blank, parallel laboratory test simultaneously.
Find through process compare with untreated sample, the content of element there occurs certain change, and different disposal change trend and amplitude different.As can be seen from Figure 2, through running water germination treatment, the content of K, Ca, Na, Sn increases more, and especially the content of Ca, Na adds 5 times and more than 2 times, and the content of Zn, B, Sr, Al, Ni, Ba, Ti decreases.
Shown by Fig. 4-Fig. 7 is the FT-IR mutation analysis of germination kidney bean freeze-dried powder.
Without the germination kidney bean freeze-dried powder of zinc sulfate and gibberellin process, as blank, the germination kidney bean freeze-dried powder of zinc sulfate and gibberellin process, as sample, is self-control; KBr powder, top grade is pure; Nicolet5700 FTIS (being equipped with OMNIC7.0 software).
Kidney bean control sample and respectively take 5.0mg with the kidney bean bud freeze-dried powder of solution of zinc sulfate process in germination process, adds KBr powder 75.0mg respectively, fully grinds in agate mortar, manual compressing tablet, measures infrared spectrum, spectral resolution 4cm
-, measurement category 4000 ~ 400cm
-, cumulative 32 times of sweep signal.
Result and analysis: infrared spectrum can Study of Molecular Structure and chemical bond, the peak position residing for ft-ir characteristic absorption peak, the strong and peak shape in peak reflects the Variation Features of certain component molecules structure.
Fig. 4 is kidney bean control sample infrared spectrogram, can be seen by Fig. 4, and kidney bean is respectively at 3285 cm
-1, 2926 cm
-1, 1643 cm
-1, 1543 cm
-1, 1396 cm
-1, 1074 cm
-1, 1050 cm
-1stronger characteristic absorption peak is there is, at 1242 cm at place
-1there is the absworption peak of moderate strength in place, in addition respectively at 1152 cm
-1990 cm
-1915 cm
-1the acromion that neighbouring appearance is stronger.Wherein 3285 cm
-1near stronger and wide band be typical associate hydrogen bond-OH stretching vibration and protein, amino acid, the stack spectral of the stretching vibration of the N-H key of peptide matters, mainly from protein, amino acid and peptide matters in kidney bean.2926cm
-1with 1396 cm
-1near be saturated first class c h bond and methylene stretching vibration absorb, mainly come from protein in kidney bean, grease, the composition such as dietary fiber and carbohydrate.1643 cm
-1with 1543 cm
-1near be respectively amide Ⅰ (C=O) and acid amides II is with the stretching vibration of (N-H, C-N) to absorb, mainly from protein and the peptides of amide structure.The absworption peak at 1242 cm places is Amide Ⅲ band is that the stretching vibration of C-N key and the flexural vibrations of N-H key absorb, the also main protein from amide structure and peptides.1074 cm, 1050 cm, there are stronger characteristic absorption peak and 1152 cm in place
-1990 cm
-1915 cm
-1the neighbouring stronger acromion occurred is that the flexible of C-O key and flexural vibrations absorb, and comes from protein, sugared and glucosides constituents.
By Fig. 5, Fig. 6 (a in Fig. 6: kidney bean control sample; B: the germination kidney bean after zinc salt Stress treatment) and Fig. 7 (in Fig. 7, dotted line is depicted as kidney bean control sample; Germination kidney bean after solid line is depicted as zinc salt Stress treatment) can see, to germinate after zinc salt Stress treatment kidney bean infrared spectrum more significant difference of existence compared with kidney bean control sample infrared spectrum, be mainly manifested in the peak position of characteristic absorption peak, peak shape, in the difference of Qiang Deng aspect, peak.These Discrepancy Descriptions, first the molecular structure of kidney bean substance in vivo there occurs change after germinateing under zinc salt Stress treatment, then the change of these molecular structures is reflected in its infrared spectrogram, so the change by investigating these characteristic absorption peaks, the change of the kidney bean substance in vivo molecular structure that germinates after can inferring zinc salt Stress treatment.
Kidney bean germinate after zinc salt Stress treatment except at 3292 cm compared with kidney bean control sample
-1, 1647 cm
-1, 1540 cm
-11261 cm
-1, 1026 cm
-1the intensity of place's characteristic absorption peak obviously strengthens, peak shape and peak position also with contrast kidney bean and have different.3292 cm
-1-OH peak is to high-frequency mobile 7 cm
-1, 1647cm
-1(amide Ⅰ) C=O peak is to high-frequency mobile 3 cm
-1, 1261 cm
-1(Amide Ⅲ band) C-N peak is to high-frequency mobile 19 cm
-1, and 3292 cm
-11261 cm
-1the remarkable grow of absorption peak strength at place, peak type narrows, and show that kidney bean is coerced in lower germination process at zinc salt and create outside certain free amino acid, in kidney bean, the content of amino acid and micromolecule polypeptide class material obviously increases.
And 1540 cm
-1(acid amides II is with) N-H, C-N peak moves 3cm to low frequency
-1, 1026 cm
-1the C-O peak of place's albumen and polysaccharide material is to again and again mobile 24 cm
-1, and 1026 cm
-1near the peak shape of the absworption peak absworption peak corresponding with kidney bean control sample by force with peak compared with there is significant difference, show that amino acid in kidney bean and micromolecule polypeptide constituents and zinc salt form a kind of stable complex bonds, cause the characteristic absorption grow of these functional groups, peak position moves to low frequency.
Detailed description of the invention
embodiment 1:select even, crack-free, pucker-free kidney bean, soak sterilizing 30 minutes with 0.1% clorox, clorox consumption was advisable not have kidney bean.Period shakes 3 times, then uses tap water rinse 5 times.Add germination nutrient solution, wherein gibberellin 35 μm of ol/L, zinc sulfate is 15mmol/L, germination temperature 28 DEG C, immersion treatment 12h, after elimination nutrient solution, place 6 hours, repeat this step 4 time, to 72 hours, stop germination treatment, homogeneous, rear freeze-drying, dried powder is manufactured goods, after testing, wherein Zn content is 2890mg/kg, can be used as dietary supplements.According to the method that general biscuit makes, add the powder described in this patent in the feed, make the content of zinc in every sheet biscuit be 5mg, namely add 1.73g germination kidney bean powder in every sheet biscuit.Preparation method is described below: 2, yolk, 2, albumen, Icing Sugar (put 13g in yolk, put 50g in albumen) 63g, weak strength flour 63g, and all raw materials claim gross mass, calculates and guarantees in every 20g raw material containing rich zinc kidney bean powder 1.73g.Add germination kidney bean powder in weak strength flour to stir.Poured into by Icing Sugar in yolk, dismiss and melt completely to Icing Sugar, albumen is dismissed to rigid foaming.Get 1/3 egg white icing and put into yolk paste, stir a little.Pour into 4 in residual protein frost and stir.Be sieved into low powder, stir gently, baking tray is extruded into finger-shaped, 190 degree, 15 minutes, obtain rich zinc finger biscuit.
embodiment 2:select even, crack-free, pucker-free kidney bean, soak sterilizing 40 minutes with 0.05% clorox, clorox consumption was advisable not have kidney bean.Period shakes 3 times, then uses tap water rinse 5 times.Add germination nutrient solution, wherein gibberellin 20 μm of ol/L, zinc sulfate is 20mmol/L, germination temperature 28 DEG C, immersion treatment 12h, after elimination nutrient solution, place 6 hours, repeat this step 4 time, to 72 hours, stop germination treatment, homogeneous, rear freeze-drying, dried powder is manufactured goods, after testing, wherein Zn content is 2890mg/kg.Pack with quantitative package machine, with meeting the double-deck Aluminum-plastic composite bag of food standard as packaging material, often packing 6g, being about 15mg containing zinc, can reconstitute with warm water time edible, supplement the approach of Zn-ef ficiency as adult.
embodiment 3:select even, crack-free, pucker-free kidney bean, soak sterilizing 30 minutes with 0.15% clorox, clorox consumption was advisable not have kidney bean.Period shakes 3 times, then uses tap water rinse 5 times.Add germination nutrient solution, wherein gibberellin 25 μm of ol/L, zinc sulfate is 45mmol/L, germination temperature 28 DEG C, immersion treatment 12h, after elimination nutrient solution, places 6 hours, repeats this step 4 time, to 72 hours, stops germination treatment.By the germination kidney bean of gained, with clear water 4 times, drain away the water, add liquid glucose and soak 2h, draining sugar liquid, packaging, sterilization is finished product, as snacks.
embodiment 4:select even, crack-free, pucker-free kidney bean, soak sterilizing 20 minutes with 0.3% clorox, clorox consumption was advisable not have kidney bean.Period shakes 3 times, then uses tap water rinse 5 times.Add germination nutrient solution, wherein gibberellin 10 μm of ol/L, zinc sulfate is 35mmol/L, germination temperature 28 DEG C, immersion treatment 12h, after elimination nutrient solution, places 6 hours, repeats this step 4 time, to 72 hours, stops germination treatment.Add the water of raw material weight 10 times, use homogenizer homogeneous, centrifugal with tube centrifuge, 10000 revs/min, get supernatant and be evaporated to relative density 1.2, spraying dry, 0.060% ascorbic acid, 0.1% citric acid is added in powder, kidney bean bud concentration is adjusted to 10%, add 0.025% sodium carboxymethylcellulose, 0.40% xanthans, 0.200% monoglyceride, the compound stabilizer of 0.050% sucrose fatty ester composition, can obtain uniform and stable, sweet taste is pure, delicate mouthfeel and have the kidney bean bud beverage of strong fragrance.
embodiment 5:select even, crack-free, pucker-free kidney bean, kidney bean was not had to soak sterilizing 30 minutes with 0.1% liquor natrii hypochloritis, period preferably shakes once for every 10 minutes, use tap water rinse afterwards 3 times, be mixed with mixed solution as nutrient solution by zinc sulfate 30mmol/L and gibberellin 20 μm of ol/L, nutrient solution is 6 by adding salt acid for adjusting pH value.Kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, at 28 DEG C, soak 12h, place 6 hours after elimination solution, repeat said process 4 times, obtain rich zinc germination kidney bean.
embodiment 6:
Select evenly, crack-free, the kidney bean of pucker-free, kidney bean was not had to soak sterilizing 24 minutes with 0.2% liquor natrii hypochloritis, the shake of every 8 minutes of period once, use tap water rinse afterwards 5 times, mixed solution is mixed with as nutrient solution by zinc sulfate 25mmol/L and gibberellin 25 μm of ol/L, add salt acid for adjusting pH value to 5.8, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 16h is soaked at 30 DEG C, place 8 hours after elimination solution, repeat said process 3 times, obtain rich zinc germination kidney bean, after the rich zinc germination kidney bean drying of above-mentioned gained, be ground into granularity≤40 object powder, as the raw material manufacturing kidney bean food further.
embodiment 7:
Select evenly, crack-free, the kidney bean of pucker-free, kidney bean was not had to soak sterilizing 40 minutes with 0.25% liquor natrii hypochloritis, the shake of every 10 minutes of period once, use tap water rinse afterwards 3 times, mixed solution is mixed with as nutrient solution by zinc sulfate 20mmol/L and gibberellin 25 μm of ol/L, add salt acid for adjusting pH value to 6, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 6h is soaked at 35 DEG C, place 2 hours after elimination solution, repeat said process 6 times, obtain rich zinc germination kidney bean, after the rich zinc germination kidney bean drying of above-mentioned gained, be ground into granularity≤60 object powder, as the raw material manufacturing kidney bean food further.
embodiment 8:
Select even, crack-free, pucker-free kidney bean, kidney bean was not had to soak sterilizing 30 minutes with 0.15% liquor natrii hypochloritis, the shake of every 10 minutes of period once, use tap water rinse afterwards 4 times, be mixed with mixed solution by zinc sulfate 15mmol/L and gibberellin 15 μm of ol/L and add salt acid for adjusting pH value to 6.1.As nutrient solution, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 10h is soaked at 32 DEG C, place 2 hours after elimination solution, 6 times, obtain rich zinc germination kidney bean, after the rich zinc germination kidney bean drying of above-mentioned gained, be ground into the powder of below 80 orders, can be used as the raw material manufacturing kidney bean food further.
embodiment 9:select evenly, crack-free, the kidney bean of pucker-free, kidney bean was not had to soak sterilizing 40 minutes with 0.2% liquor natrii hypochloritis, the shake of 8 minutes periods once, use tap water rinse afterwards 3 times, mixed solution is mixed with as nutrient solution by zinc sulfate 18mmol/L and gibberellin 22 μm of ol/L, add salt acid for adjusting pH value to 6.1, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 8h is soaked at 26 DEG C, place 4 hours after elimination solution, repeat said process 5 times, obtain rich zinc germination kidney bean, the rich zinc germination kidney bean of gained is dry and be ground into the powder of below granularity 100 order, as the raw material manufacturing kidney bean food further.
embodiment 10:
Select evenly, crack-free, the kidney bean of pucker-free, kidney bean was not had to soak sterilizing 20 minutes with 0.35% liquor natrii hypochloritis, period shakes once when often spending 8 minutes, tap water rinse 3 times are used after 20 minutes, mixed solution is mixed with as nutrient solution by zinc sulfate 16mmol/L and gibberellin 20 μm of ol/L, add salt acid for adjusting pH value to 5.9, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 20h is soaked at 28 DEG C, place 4 hours after elimination solution, repeat said process 3 times, obtain rich zinc germination kidney bean, the rich zinc germination kidney bean crushed after being dried of above-mentioned gained is become the powder of below granularity 120 order, as the raw material manufacturing kidney bean food further.
embodiment 12:
Select even, crack-free, pucker-free kidney bean, kidney bean was not had to soak sterilizing 20 ~ 40 minutes with 0.05% liquor natrii hypochloritis, the shake of every 12 minutes of period once, use tap water rinse afterwards 3 times, be mixed with mixed solution as nutrient solution by zinc sulfate 40mmol/L and gibberellin 15 μm of ol/L, add salt acid for adjusting pH value to 5.8.Kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 8h is soaked at 30 DEG C, place 4 hours after elimination solution, repeat said process 6 times, obtain rich zinc germination kidney bean, after the rich zinc germination kidney bean drying of gained, be ground into granularity≤80 object powder, as the raw material manufacturing kidney bean food further.
embodiment 13:
Select even, crack-free, pucker-free kidney bean, kidney bean was not had to soak sterilizing 20 minutes with 0.5% liquor natrii hypochloritis, the shake of every 8 minutes of period once, use tap water rinse afterwards 5 times, be mixed with mixed solution as nutrient solution by zinc sulfate 25mmol/L and gibberellin 20 μm of ol/L, add salt acid for adjusting pH value to 6.Kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, at 25 DEG C, soak 8h, place 4 hours after elimination solution, repeat said process 5 times, obtain rich zinc germination kidney bean.The rich zinc germination kidney bean crushed after being dried of above-mentioned gained becomes the powder of below granularity 80 order, as the raw material manufacturing kidney bean food further.
embodiment 14:select even, crack-free, pucker-free kidney bean, kidney bean was not had to soak sterilizing 30 minutes with 0.3% liquor natrii hypochloritis, period preferably shakes once for every 8 minutes, use tap water rinse afterwards 3 times, be mixed with mixed solution as nutrient solution by zinc sulfate 30mmol/L and gibberellin 10 μm of ol/L, add salt acid for adjusting pH value to 5.9.Kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 12h is soaked at 15 DEG C, place 6 hours after elimination solution, repeat said process 4 times, obtain rich zinc germination kidney bean, the rich zinc germination kidney bean crushed after being dried of above-mentioned gained becomes granularity≤60 object powder, as the raw material manufacturing kidney bean food further.
embodiment 15:select even, crack-free, pucker-free kidney bean, kidney bean was not had to soak sterilizing 30 minutes with 0.35% liquor natrii hypochloritis, period preferably shakes once for every 10 minutes, use tap water rinse afterwards 3 times, mixed solution is mixed with as nutrient solution by zinc sulfate 20mmol/L and gibberellin 15 μm of ol/L, add salt acid for adjusting pH value to 6, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 18h is soaked at 22 DEG C, place 6 hours after elimination solution, repeat said process 3 times, obtain rich zinc germination kidney bean.The rich zinc germination kidney bean crushed after being dried of above-mentioned gained becomes granularity≤40 object powder, as the raw material manufacturing kidney bean food further.
Claims (10)
1. a production method for rich zinc kidney bean goods, is characterized in that, comprise following steps:
Get the raw materials ready: select even, crack-free, pucker-free kidney bean;
Sterilizing: soak sterilizing 20 ~ 40 minutes with 0.05 ~ 0.5% liquor natrii hypochloritis, clean with tap water rinse afterwards;
Germinate: be mixed with mixed solution as nutrient solution by zinc sulfate 5 ~ 60mmol/L and gibberellin 10 ~ 60 μm of ol/L, kidney bean after sterilizing is joined in the mixed-culture medium of zinc sulfate and gibberellin, 6 ~ 24h is soaked at 15 ~ 35 DEG C, place 2 ~ 10 hours after elimination solution, repeat said process 2 ~ 6 times, obtain rich zinc germination kidney bean.
2. the production method of rich zinc kidney bean goods according to claim 1, is characterized in that: during described sterilizing, shake in every 8 ~ 12 minutes once.
3. the production method of rich zinc kidney bean goods according to claim 1 and 2, is characterized in that: described nutrient solution is zinc sulfate 10 ~ 40mmol/L and gibberellin 10 ~ 45 μm of ol/L.
4. the production method of rich zinc kidney bean goods according to claim 1 and 2, is characterized in that: described nutrient solution is zinc sulfate 15 ~ 20mmol/L and gibberellin 15 ~ 25 μm of ol/L.
5. the production method of rich zinc kidney bean goods according to claim 1 and 2, is characterized in that: described germination temperature is 18 ~ 32 DEG C.
6. the production method of rich zinc kidney bean goods according to claim 3, is characterized in that: described germination temperature is 18 ~ 32 DEG C.
7. the production method of rich zinc kidney bean goods according to claim 4, is characterized in that: described germination temperature is 18 ~ 32 DEG C.
8. the production method of the rich zinc kidney bean goods according to any one of claim 1-7, is characterized in that: add salt acid for adjusting pH value to 5.8 ~ 6.2 in described nutrient solution.
9. rich zinc kidney bean goods, it is characterized in that, according to any one of claim 1 to 8, method prepares gained.
10. rich zinc kidney bean goods, is characterized in that: after the rich zinc germination kidney bean drying of claim 9 gained, be ground into powder, as the raw material manufacturing kidney bean food further.
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