CN104284901A - 使用有机溶剂中的枯草杆菌蛋白酶的侧链被保护的寡肽片段缩合 - Google Patents

使用有机溶剂中的枯草杆菌蛋白酶的侧链被保护的寡肽片段缩合 Download PDF

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Publication number
CN104284901A
CN104284901A CN201380022510.2A CN201380022510A CN104284901A CN 104284901 A CN104284901 A CN 104284901A CN 201380022510 A CN201380022510 A CN 201380022510A CN 104284901 A CN104284901 A CN 104284901A
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CN
China
Prior art keywords
oligopeptide
amino acid
protected
terminal
side chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN201380022510.2A
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English (en)
Chinese (zh)
Inventor
彼得·贾恩·莱纳德·马里奥·库埃迪弗利艾格
蒂莫·努伊金斯
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Enzypep BV
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Enzypep BV
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Publication date
Application filed by Enzypep BV filed Critical Enzypep BV
Publication of CN104284901A publication Critical patent/CN104284901A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/026—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution by fragment condensation in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/042—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers characterised by the nature of the carrier

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201380022510.2A 2012-02-29 2013-02-28 使用有机溶剂中的枯草杆菌蛋白酶的侧链被保护的寡肽片段缩合 Pending CN104284901A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12157571.6 2012-02-29
EP12157571.6A EP2634193B1 (en) 2012-02-29 2012-02-29 Side-chain protected oligopeptide fragment condensation using subtilisins in organic solvents
PCT/NL2013/050125 WO2013129926A1 (en) 2012-02-29 2013-02-28 Side-chain protected oligopeptide fragment condensation using subtilisins in organic solvents

Publications (1)

Publication Number Publication Date
CN104284901A true CN104284901A (zh) 2015-01-14

Family

ID=47884473

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380022510.2A Pending CN104284901A (zh) 2012-02-29 2013-02-28 使用有机溶剂中的枯草杆菌蛋白酶的侧链被保护的寡肽片段缩合

Country Status (9)

Country Link
US (1) US9598714B2 (https=)
EP (1) EP2634193B1 (https=)
JP (1) JP2015508666A (https=)
CN (1) CN104284901A (https=)
CA (1) CA2864865A1 (https=)
DK (1) DK2634193T3 (https=)
ES (1) ES2531054T3 (https=)
IN (1) IN2014MN01770A (https=)
WO (1) WO2013129926A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5758499B2 (ja) * 2010-12-21 2015-08-05 シージェイ チェイルジェダン コーポレイション ホモセリンアセチルトランスフェラーゼ活性を有する変異型ポリペプチド及びそれを発現する微生物
WO2016056913A1 (en) 2014-10-10 2016-04-14 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
US10752931B2 (en) * 2015-07-09 2020-08-25 Enzypep B.V. Designing an enzymatic peptide fragment condensation strategy
US10827880B2 (en) 2016-02-03 2020-11-10 Conair Corporation Food processor and modular lid assembly therefor
CA3108931C (en) * 2018-08-09 2023-11-07 F. Hoffmann-La Roche Ag Cleavable linker for peptide synthesis
US20250043240A1 (en) * 2021-11-12 2025-02-06 Virginia Tech Intellectual Properties, Inc. Hydrolyzed protein serum replacement compositions
KR102886982B1 (ko) * 2022-03-11 2025-11-17 강성구 고상 유기 합성용 기재

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL6509727A (https=) * 1965-07-28 1967-01-30
DE602006008625D1 (de) * 2005-04-01 2009-10-01 Intezyne Technologies Inc Polymermicellen für die arzneistoffzufuhr
EA019536B1 (ru) * 2008-11-19 2014-04-30 ДСМ АйПи АССЕТС Б.В. Синтез пептидов с использованием энзиматической активации и конденсации

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BJORUP P. 等: "Reaction Medium Engineering in Enzymatic Peptide Fragment Condensation: Synthesis of Eledoisin and LH-RH", 《BIOORGANIC & MEDICINAL CHEMISTRY》, 31 December 1998 (1998-12-31) *
NUIJENS T. 等: "Enzymatic synthesis of activated esters and their subsequent use in enzyme-based peptide synthesis", 《JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC》, 5 April 2011 (2011-04-05) *

Also Published As

Publication number Publication date
ES2531054T3 (es) 2015-03-10
EP2634193A1 (en) 2013-09-04
US20150037840A1 (en) 2015-02-05
WO2013129926A1 (en) 2013-09-06
CA2864865A1 (en) 2013-09-06
DK2634193T3 (en) 2015-03-02
IN2014MN01770A (https=) 2015-07-03
US9598714B2 (en) 2017-03-21
JP2015508666A (ja) 2015-03-23
EP2634193B1 (en) 2015-01-14

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Application publication date: 20150114