CN104274584A - Preparation method of Tibetan drug for treating alopecia - Google Patents
Preparation method of Tibetan drug for treating alopecia Download PDFInfo
- Publication number
- CN104274584A CN104274584A CN201410576016.7A CN201410576016A CN104274584A CN 104274584 A CN104274584 A CN 104274584A CN 201410576016 A CN201410576016 A CN 201410576016A CN 104274584 A CN104274584 A CN 104274584A
- Authority
- CN
- China
- Prior art keywords
- hair
- tibetan medicine
- group
- preparation
- alopecia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/55—Linaceae (Flax family), e.g. Linum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/80—Scrophulariaceae (Figwort family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/08—Solutions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Dispersion Chemistry (AREA)
- Biomedical Technology (AREA)
- Rheumatology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a preparation method of a Tibetan drug for treating alopecia. The preparation method comprises the step of preparing an active component from buffalo horns, walnut epicarp, rumex acetosa, black sesame, fiberflax and fresh pedicularis according to a certain weight ratio by using a mixing and grinding method or a water extraction and alcohol precipitation method or an alcohol extraction and water precipitation method to obtain the Tibetan drug which can be prepared into any one common dosage form for external use. The Tibetan drug has the functions of promoting blood circulation to remove blood stasis as well as nourishing blood and promoting hair growth and is used for treating oily scalp, desquamation, itching, hypotrichosis, withered and yellow hair, bifurcate hair, fine and soft hair, easily-breaking hair, alopecia, psilosis, premature graying hair and the like.
Description
Technical field
The present invention relates to a kind of preparation method of hair growth Tibetan medicine, belong to Tibetan medicine field.
Background technology
Alopecia refers to the abnormal phenomenon come off of hair, and its reason is a lot, common are seborrheica (i.e. male type) alopecia; Nervous alopecia and pathologic alopecia.Seborrheic alopecia (Seobrrheic Alopecia, SA) is called for short seborrheic alopecia, has another name called male pattern alopecia, androgenetic alopecia, clinically shows as the gradual alopecia that hair is greasy, consider more, have obvious scratchiness, E Nie district and top to be worth doing more, then forms great number.
This disease is commonly encountered diseases, the frequently-occurring disease of department of dermatologry, is also one of refractory disease.Under normal circumstances, a healthy adult every day can alopecia 50 ~ 75.If alopecia every day more than 100, and continue more than 30 days, can be made a definite diagnosis and suffer from alopecia.
World Health Organization (WHO) delivers report: alopecia and poliosis are becoming a global problem.Investigate according to national authority health organ: the number of domestic alopecia, alopecia, withered and yellow, bifurcated, jewelry, expensive clothing and other valuables, poliosis is more than 1.5 hundred million people; China 25 ~ 35 years old man suffers from the ratio of male pattern alopecia up to 40%, and 84.8% is diagnosed as seborrheic alopecia.Cause alopecia because atmospheric pollution, social pressure increasing and dietary structure are uneven etc., the people of poliosis gets more and more, and the crowd of alopecia tends to rejuvenation.
The growth of hair and regeneration depend on the enough nutrition blood supplies to hair follicle.Microcirculatory perfusion obstacle causes the flow velocity of hemoperfusion in microcirculation and flow to decline, and the normal nutrition and the oxygen supply that affect scalp hair follicles reduce, thus cause the generation of alopecia.Medicine by raising skin temperature, can skin expansion blood vessel, improve skin microcirculation, increase hair papilla blood supply, improve hair follicle nutrition, promote that tissue is newborn, and then promote hair growth.
Although the medicine of current hair growth is a lot, curative effect still can not be satisfactory.Therefore, there is tight demand in the Tibetan medicine of people to the better hair growth of curative effect.
Up to now, any report about Tibetan medicinal composition of the present invention and preparation method thereof is not also found.The present inventor through repeatedly studying, and by the checking repeatedly of animal and clinical trial, finally have found safe, have the oral thing and preparation method thereof of taking medicine of the Tibetan medicine of the hair growth of better curative effect, thus complete the present invention.
Summary of the invention
The object of the invention is just to provide a kind of preparation method of more effective hair growth Tibetan medicine.
Tibetan medicine of the present invention is made up of active component or is made up of active component and pharmaceutically acceptable adjuvant, and wherein said active component is made up of following bulk drugs: Cornu Bubali, walnut shell, Radix rumicis acetosae, Semen Sesami Nigrum, Caulis et Folium Lini, Radix pedicularis muscicolae.
It has selected Cornu Bubali, walnut shell, Radix rumicis acetosae, Semen Sesami Nigrum, Caulis et Folium Lini, Radix pedicularis muscicolae carry out combining as crude drug, and wherein (1) Cornu Bubali is the angle of bovid Babalus bubalis L. Bubalus bubalis Linnaeus.There is inducing diuresis to remove edema, effect that repiece is sent out.For cold, hot two kinds of edema caused and alopecia disease.(2) walnut shell is the outer peel of juglandaceae plant Semen Juglandis Juglans regia L..There is black hair, effect of hair growth promoting.For poliosis, alopecia disease.(3) Radix rumicis acetosae is the dry root of polygonaceae plant Nepal Radix rumicis acetosae Rumex nepalensis Spreng.There is heat clearing away, damp eliminating, detumescence, more effect of skin ulcer.For furuncle, eczema.(4) Semen Sesami Nigrum is the dry mature seed of Pedaliaceae plant sesame Sesamum indicum L..There is invigorating the liver and kidney, benefiting essence-blood, effect that intestine moistening is dry.For dizziness and blurred vision, Hiccough and deaf, early whitening of beard and hair, post disease alopecia, dryness of the intestine constipation.(5) Caulis et Folium Lini is the dry mature seed of flax family (Linaceae) plant Caulis et Folium Lini Linum usitatissimum L..Have and moisturize, the effect of dispeling the wind.For dryness of the intestine constipation, xerosis cutis pruritus, hair is withered to come off.(6) Radix pedicularis muscicolae is the dried floral of goatweed Radix pedicularis muscicolae Pedicularis muscicola Maxim..Have and hold back poison, heat clearing away, effect of growing and blacking hair.For meat poison, Baconic's wood brucellosis, hot diarrhoea, painful abdominal mass tumor, pneumonopathy, disorders of meridian.These crude drug are combinationally used and makes each crude drug effect produce synergism, thus can effective hair growth.
The consumption of Tibetan medicine active component of the present invention is also through inventor and gropes in a large number to sum up and draw, each crude drug consumption for all to have good therapeutic effect within the scope of following concrete weight proportion:
Cornu Bubali 10 ~ 30g, walnut shell 15 ~ 45g, Radix rumicis acetosae 7.5 ~ 22.5g, Semen Sesami Nigrum 7.5 ~ 22.5g, Caulis et Folium Lini 10 ~ 30g, Radix pedicularis muscicolae 15 ~ 45g.
Be preferably: Cornu Bubali 16 ~ 24g, walnut shell 24 ~ 36g, Radix rumicis acetosae 12 ~ 18g, Semen Sesami Nigrum 12 ~ 18g, Caulis et Folium Lini 16 ~ 24g, Radix pedicularis muscicolae 24 ~ 36g.
More preferably: Cornu Bubali 20g, walnut shell 30g, Radix rumicis acetosae 15g, Semen Sesami Nigrum 15g, Caulis et Folium Lini 20g, Radix pedicularis muscicolae 30g.
The preparation of Tibetan medicine active component of the present invention can be pulverized by the crude drug convection drying of above-mentioned consumption to make; Also the crude drug of above-mentioned consumption can be adopted the conventional method of Chinese medicine preparation such as decoction and alcohol sedimentation technique or ethanol extract from water precipitation (see " pharmaceutics of Chinese drugs " 73rd ~ 74 pages of Cao Chunlin chief editor, Science and Technology of Shanghai publishing house publishes in November, 1986) to obtain.
The active component of Tibetan medicine of the present invention can add various customary adjuvant required when preparing different dosage form, as suitable substrate, suitable solvent, suitable propellant etc. with the method for Chinese medicinal of routine (see " pharmaceutics of Chinese drugs " of Cao Chunlin chief editor, Science and Technology of Shanghai publishing house publishes in November, 1986) be prepared into any one common formulations, as tincture, fluid extract, extractum, ointment, liniment, lotion, aerosol, spray etc.
Tibetan medicine of the present invention has the function of blood circulation promoting and blood stasis dispelling, blood nourishing and hair., desquamation, pruritus greasy for scalp, hypotrichosis, withered and yellow, bifurcated, jewelry, expensive clothing and other valuables, hair easily fractures, alopecia, alopecia, early whitening of beard and hair etc.
The usage and dosage of Tibetan medicine of the present invention is: external; Embrocate affected part, massage 3 ~ 5 minutes to affected part heating, 2 ~ 3 times on the one.
[detailed description of the invention]
Carry out the preparation method setting forth Tibetan medicine of the present invention further by the following examples.
The preparation of [embodiment 1] Tibetan medicine tincture of the present invention:
Take Cornu Bubali 20g, walnut shell 30g, Radix rumicis acetosae 15g, Semen Sesami Nigrum 15g, Caulis et Folium Lini 20g, Radix pedicularis muscicolae 30g, be ground into coarse powder, make solvent with 75% ethanol, Mi Gai, time add stirring, flood seven, get supernatant, filter, squeezing medicinal residues, press liquor and filtrate merge, stir evenly, leave standstill 24 hours, filter, subpackage, obtains tincture.
The preparation of [embodiment 2] Tibetan medicine fluid extract of the present invention:
Take Cornu Bubali 10g, walnut shell 15g, Radix rumicis acetosae 7.5g, Semen Sesami Nigrum 7.5g, Caulis et Folium Lini 10g, Radix pedicularis muscicolae 15g, be ground into coarse powder, solvent is made with 70% ethanol, to flood after 48 hours slowly percolation, collect percolate, be concentrated into relative density 1.05 ~ 1.10(25 DEG C), leave standstill 24 hours, get supernatant, subpackage, obtain fluid extract.
The preparation of [embodiment 3] Tibetan medicine extractum of the present invention:
Take Cornu Bubali 30g, walnut shell 45g, Radix rumicis acetosae 22.5g, Semen Sesami Nigrum 22.5g, Caulis et Folium Lini 30g, Radix pedicularis muscicolae 45g, be ground into coarse powder, solvent is made with 70% ethanol, to flood after 48 hours slowly percolation, collect percolate, be concentrated into relative density 1.25 ~ 1.30(25 DEG C) thick paste, subpackage, obtains extractum.
The preparation of [embodiment 4] Tibetan medicine ointment of the present invention:
Take Cornu Bubali 16g, walnut shell 24g, Radix rumicis acetosae 12g, Semen Sesami Nigrum 12g, Caulis et Folium Lini 16g, Radix pedicularis muscicolae 24g, be jointly ground into impalpable powder after mixing, mixing, adds ointment base, and stir evenly to semisolid dress, subpackage, obtains ointment.
The preparation of [embodiment 5] Tibetan medicine liniment of the present invention:
Take Cornu Bubali 24g, walnut shell 36g, Radix rumicis acetosae 18g, Semen Sesami Nigrum 18g, Caulis et Folium Lini 24g, Radix pedicularis muscicolae 36g, be ground into coarse powder, make solvent with 75% ethanol, Mi Gai, time add stirring, flood seven, get supernatant, filter, squeezing medicinal residues, press liquor and filtrate merge, stir evenly, leave standstill 24 hours, filter, subpackage, obtains liniment.
The preparation of [embodiment 6] Tibetan medicine lotion of the present invention:
Take Cornu Bubali 14g, walnut shell 21g, Radix rumicis acetosae 10.5g, Semen Sesami Nigrum 10.5g, Caulis et Folium Lini 14g, Radix pedicularis muscicolae 21g, be ground into coarse powder, by appropriate water boiling and extraction 3 times, each 1 hour, filter, merge 3 filtrates, be concentrated into relative density 1.10 ~ 1.15(25 DEG C), subpackage, obtains lotion.
The preparation of [embodiment 7] Tibetan medicine aerosol of the present invention:
Take Cornu Bubali 26g, walnut shell 39g, Radix rumicis acetosae 19.5g, Semen Sesami Nigrum 19.5g, Caulis et Folium Lini 26g, Radix pedicularis muscicolae 39g, be ground into coarse powder, by appropriate water boiling and extraction 3 times, each 1 hour, filter, merge 3 filtrates, be concentrated into relative density 1.10 ~ 1.15(25 DEG C), add suitable propellant, mixing, be packaged in suitable container, obtain aerosol.
The spray preparation of [embodiment 8] Tibetan medicine of the present invention:
Take Cornu Bubali 28g, walnut shell 42g, Radix rumicis acetosae 21g, Semen Sesami Nigrum 21g, Caulis et Folium Lini 28g, Radix pedicularis muscicolae 42g, be ground into coarse powder, by appropriate water boiling and extraction 3 times, each 1 hour, filter, merge 3 filtrates, be concentrated into relative density 1.05 ~ 1.15(25 DEG C), be packaged in suitable container, obtain spray.
Set forth the beneficial effect of Tibetan medicine of the present invention further below by way of test example, these test examples include pharmacodynamics test and the clinical observation on the therapeutic effect test of Tibetan medicine embodiment 1 tincture of the present invention.
[test example 1] Tibetan medicine embodiment 1 tincture of the present invention is to the pharmacodynamics test of mouse skin temperature and auricle Microcirculation Effect:
Test material: anthology invention Tibetan medicine embodiment 1 tincture; 101 HAIR BREEDING AGENT (Beijing Zhangguang 101 Technology Co., Ltd., product batch number: 20101001); 95% ethanol (Tianjin Fu Yu Fine Chemical Co., Ltd, product batch number: 100312); Pentobarbital (chemical experimental factory of Foshan City, product batch number: 20100406); Sodium chloride injection (Shandong Lukang Cisen Pharmaceutical Co., Ltd, product batch number: 1004234202); Picric acid (Guangzhou Chemical Reagent Factory, product batch number: 20090411); Sodium sulfide (Fanyu, Guangzhou power strengthening factory, product batch number: 20100706); NIH mice is provided by Qinghai Province's Experimental Animal Center.
Experimental technique and result: 1, get mice 50, male and female half and half.Back wool is cut short, by depilatory (6%Na
2s) be applied to mouse back, depilation area is with warm water cleaning (smooth with mouse back, to be clean without hindering without residual hair) after 3cm × 4cm, 3min.Mice is divided into 5 groups by next day at random, each 10, is respectively blank group (75% ethanol group), positive controls (101 HAIR BREEDING AGENT group), the basic, normal, high dosage group of Tibetan medicine embodiment 1 tincture of the present invention, smear relative medicine in each group of mice depilation district, dosage is in table 1.Smear 1 time, with infrared radiation thermometer measure each group of mice different time points (15,30,60,120min) the forward and backward skin temperature of administration.Laboratory temperature is 19 ~ 21 DEG C, the results are shown in Table 1, table 2, table 3, table 4.
Separate groups of mice skin temperature change (x ± s, n=10) after table 1 administration 15min
Group Animals number dosage (ml/ only) temperature gap
Blank group 10 0.2-2.93 ± 3.21
Positive controls 10 0.2-0.49 ± 2.26
Low dose group 10 0.1 1.23 ± 2.53
*
Middle dosage group 10 0.2-0.39 ± 3.56
High dose group 10 0.4-1.32 ± 3.47
Illustrate: compare with blank group:
*p<0.01.
Table 1 result shows, after administration 15min, due in medicinal liquid all containing a certain amount of ethanol, ethanol volatilizees and takes away the heat of skin, the therefore equal decrease to some degree of each treated animal skin temperature.Compare with blank group matched group, Tibetan medicine embodiment 1 tincture low dose group dosage of the present invention is few, and therefore mouse skin temperature reduces not obvious, and shows the situation (P < 0.01) that temperature significantly raises; Positive controls, Tibetan medicine embodiment 1 tincture of the present invention high, middle dosage group mouse skin temperature all decreases, but reduction amplitude is all less than blank group, prompting medicine can resist ethanol to a certain extent and volatilize the skin temperature reduction caused, but there was no significant difference (P > 0.05).Compare with positive controls, the high, medium and low dosage group of Tibetan medicine embodiment 1 tincture of the present invention there are no significant difference (P > 0.05).
Separate groups of mice skin temperature change (x ± s, n=10) after table 2 administration 30min
Group Animals number dosage (ml/ only) temperature gap
Blank group 10 0.2-2.23 ± 3.12
Positive controls 10 0.2 0.43 ± 4.86
Low dose group 10 0.1 1.37 ± 1.26
*
Middle dosage group 10 0.2-1.11 ± 3.58
High dose group 10 0.4 0.39 ± 3.19
Illustrate: compare with blank group:
*p<0.05.
Table 2 result shows, after administration 30min, the skin temperature of blank group is still lower than level before administration, compare with blank group, level before positive controls and Tibetan medicine embodiment 1 tincture high dose group mouse skin temperature return to administration of the present invention, but still with blank group there was no significant difference (P > 0.05); In Tibetan medicine embodiment 1 tincture of the present invention, dosage group mouse skin temperature slightly reduces, and reduces Amplitude Ratio blank group little, but there was no significant difference (P > 0.05); Tibetan medicine embodiment 1 tincture low dose group mouse skin temperature of the present invention is significantly higher than blank group (P < 0.05), and it is relevant that and medicine less than normal to dosage resists factor; Compare with positive controls, the high, medium and low dosage group of Tibetan medicine embodiment 1 tincture of the present invention there are no significant difference (P > 0.05).
Separate groups of mice skin temperature change (x ± s, n=10) after table 3 administration 60min
Group Animals number dosage (ml/ only) temperature gap
Blank group 10 0.2-3.23 ± 3.46
Positive controls 10 0.2-0.98 ± 4.26
Low dose group 10 0.1 0.89 ± 2.42
*
Middle dosage group 10 0.2-0.27 ± 2.63
High dose group 10 0.4 0.59 ± 3.76
*
Illustrate: compare with blank group:
*p<0.05.
Table 3 result shows, after administration 60min, the skin temperature of blank group is still lower than level before administration, compare with blank group, in positive controls and Tibetan medicine embodiment 1 tincture of the present invention, dosage group mouse skin temperature is a little less than before administration, but reduction amplitude is less than blank group, both there was no significant differences (P > 0.05); Tibetan medicine embodiment 1 tincture high and low dose group mouse skin temperature of the present invention is significantly elevated to higher than level before administration (P < 0.05); Compare with positive controls, the high, medium and low dosage group of Tibetan medicine embodiment 1 tincture of the present invention there are no significant difference (P > 0.05).
Separate groups of mice skin temperature change (x ± s, n=10) after table 4 administration 120min
Group Animals number dosage (ml/ only) temperature gap
Blank group 10 0.2-2.32 ± 1.21
Positive controls 10 0.2 1.34 ± 3.43
*
Low dose group 10 0.1 1.21 ± 0.52
*
Middle dosage group 10 0.2 0.83 ± 2.63
*
High dose group 10 0.4 3.89 ± 2.16
* #
Illustrate: compare with blank group:
*p<0.01; Compare by positive controls:
#p<0.05.
Table 4 result shows, and after administration 120min, the skin temperature of blank group is still lower than level before administration, and positive controls significantly can raise mouse skin temperature (P < 0.01), prompting modeling success; And Tibetan medicine embodiment 1 tincture of the present invention high, medium and low dosage group mouse skin temperature significantly raises (P < 0.01).Compare with positive controls, Tibetan medicine embodiment 1 tincture high dose group skin temperature of the present invention significantly raises, there is significant difference (P < 0.05), point out Tibetan medicine embodiment 1 tincture high dose group of the present invention significantly can improve the microcirculation of local skin, improve skin temperature, and drug effect is significantly better than positive controls.
2, Tibetan medicine embodiment 1 tincture of the present invention is to Mice Auricle Microcirculation Effect:
Get mice 50, male and female half and half.Be divided into blank group (75% ethanol) at random, positive controls, the basic, normal, high dosage group of Tibetan medicine embodiment 1 tincture of the present invention.Measure Mice Auricle microcirculatory blood flow (PU) by after mouse anesthesia with laser-Doppler microcirculation instrument, in each group of Mice Auricle, smear relative medicine, dosage is in table 5.Again measure the blood flow (PU) observing each group of mice after 20min, the PU difference of each group is compared, the results are shown in Table 5.
Table 5 Tibetan medicine of the present invention embodiment 1 tincture is on the microcirculatory impact of Mice Auricle (x ± s, n=10)
Group Animals number dosage (μ l/ only) PU difference
Blank group 10 80-1.37 ± 4.16
Positive controls 10 80 25.98 ± 9.76
*
Low dose group 10 40 28.72 ± 15.36
*
Middle dosage group 10 80 28.93 ± 11.24
*
High dose group 10 160 23.15 ± 12.64
*
Illustrate: compare with blank group:
*p<0.01.
Table 5 result shows, and compares before administration and after administration, and blank group Mice Auricle microcirculation PU value is substantially without change; Compare with blank group, positive controls can significantly improve blood capillary PU value, improves Mice Auricle microcirculation, accelerates blood flow rate (P < 0.01), prompting modeling success.Compare with blank group, the high, medium and low dosage group of Tibetan medicine embodiment 1 tincture of the present invention all significantly can improve Mice Auricle microcirculation (P < 0.01), the high, medium and low dosage group of Tibetan medicine embodiment 1 tincture of the present invention is pointed out all can significantly to increase Mice Auricle microcirculation, expansion blood capillary, accelerates blood flow rate; Compare with positive controls, in Tibetan medicine embodiment 1 tincture of the present invention, low dose group PU value increase level is higher than positive controls, but there was no significant difference (P > 0.05), point out Tibetan medicine embodiment 1 tincture of the present invention to improve microcirculation level and be slightly better than positive controls, but there was no significant difference; There was no significant difference (P > 0.05) between the high, medium and low dosage group of Tibetan medicine embodiment 1 tincture of the present invention, points out Tibetan medicine embodiment 1 tincture of the present invention to there is not obvious dose dependent to the microcirculatory drug effect of Mice Auricle.
Androgenetic alopecia and hemorheology change and there is dependency, and blood viscosity increases, syndrome of blood stasis may be one of pathogenesis of primary disease.Research finds, the high relative indices of cutting of androgenetic alopecia patient whole blood viscosity (being cut to master with height), packed cell volume, whole blood is apparently higher than Normal group." hair is the sign of blood condition ", alopecia is in close relations with " blood ".Blood viscosity is higher, and blood fluidity is less, otherwise larger.Blood viscosity increase can cause blood circulation and microcirculation disturbance, causes tissue to fill with a series of consequences such as insufficiency of blood, hypoxic-ischemic, metabolic function imbalance.From modern medicine angle, microcirculation disturbance makes perifollicolar vasoconstriction, stimulates vascular smooth muscle cell proliferation, finally makes ischemic hypoxia, cause hair follicle Progressive symmetric erythrokeratodermia to reduce.Originally experimental studies have found that, Tibetan medicine embodiment 1 tincture of the present invention has rising mouse skin temperature, and blood vessel dilating, improves microcirculatory effect.Point out Tibetan medicine embodiment 1 tincture energy skin expansion blood vessel of the present invention, improve skin microcirculation, increase hair papilla blood supply, improve hair follicle nutrition, thus promote hair growth.
[test example 2] Tibetan medicine embodiment 1 tincture of the present invention hair growth clinical observation on the therapeutic effect:
1, physical data: patient, all from Dermatology Outpatient Department, is divided into two groups by SAS statistical analysis system by 120 routine patients at random, treatment group 60 example, man 49 example, female 11 example, 20 ~ 49 years old age, the course of disease 3 months ~ 2 years, average 8.5 months; Matched group 60 example, man 47 example, female 13 example, 18 ~ 47 years old age, the course of disease 3 months ~ 2 years, average 9.3 months.Two groups, at age, the course of disease statistically no significant difference (P>0.05), have comparability.
2, diagnostics and maintenance standard:
(1) diagnostic criteria: 1. fat secretion is vigorous, hair is greasy, or the dandruff is obvious, and hair is dry, with pruritus; 2. volume anterior hair line line constantly raises; 3. alopecia is continuously more than 3 months, and alopecia every day number is more than 100; 4. hair is tapered deliquescing, in rarefaction state.Allly meet above two standard persons, can androgenetic alopecia be diagnosed as.
(2) exclusion standard: 1. the age is below 18 years old; 2. other endocrine alopecias (as hypothyroidism or hyperfunction, parathyroid gland or primary pituitary hypofunction, hypogonadism, diabetes etc.); 3. alopecia medicamentosa person, symptomatic alopecia person, women's puerperal alopecia; 4. inclusive criteria or data not umbra sound statistical analysis person is not met.
3, Therapeutic Method: treatment group adopts Tibetan medicine embodiment 1 tincture of the present invention, embrocates affected part, massages 3 ~ 5 minutes to affected part heating, 2 ~ 3 times on the one; Percussopunctator head buckles thorn, 3 days/time, each 15min; It within 60 days, is 1 course for the treatment of.
Matched group selects climing enlightening, every day 2 times, each 1ml, outer spray, and smears from center, affected part, with hands massage 3 ~ 5min; Percussopunctator head buckles thorn, 3 days/time, each 15min; It within 60 days, is 1 course for the treatment of.
All treat 3 courses for the treatment of, after 6 months, judge curative effect for two groups.Every two weeks record 1 skin lesion place hair growth situation, observes with or without systemic adverse reactions (comprise heating, headache, weak etc.).
4, clinical efficacy evaluation criteria: the criterion of therapeutical effect of the propositions such as the therapeutic effect reference WEISS of hair.
(1) fully recover: new sending out all grows, and distribution density, hair thickness, the same normal hair of color and luster, gently draw negative;
(2) effective: kainogenesis long more than 50%, has more fine hair on birds or animals hair to become terminal hair, gently draw negative;
(3) effective: kainogenesis grew for more than 10% (comprising fine hair on birds or animals hair), but poor growth, gently draw negative or the positive;
(4) invalid: to continue to come off lower than while 10% or growth without kainogenesis length or kainogenesis length.
(5) simultaneous phenomenon standards of grading, select the main simultaneous phenomenon of primary disease three (scalp greasy, scalp itch, dandruff) to observe.The point system of the greasy degree of scalp, scalp itch degree and dandruff quantitative degree uses for reference visual analogue scales (the Visual Analogue Scale in pain therapy, VAS): draw one scale, 11 numerals 0 ~ 10 are evenly marked on chi as scale, by patient according to the corresponding mark of autoscopia labelling on scale.0 point=nothing, 1 ~ 3 point=slight, 4 ~ 7 points=moderate, 8 ~ 10 points=severe.
Recovery from illness adds and effectively adds up to total effective rate.
Clinical observation result is in table 6, table 7, table 8.
Table 6 liang group distinct methods treatment androgenetic alopecia comparitive study (example, %)
Group number of cases is fully recovered effective enabledisable cure rate total effective rate
Treatment group 60 24 19 98 40.00 71.67
Matched group 60 19 14 15 12 31.67 55.00
Table 6 result shows, and cure rate compares significant difference (P<0.05), and total effective rate comparing difference has significance (P<0.05), shows that treatment group curative effect is better than matched group.Study the prolongation all found with the androgenetic alopecia patient course of disease for two groups, its cure rate and effective percentage decline gradually.
A table 7 liang group distinct methods treatment androgenetic alopecia onset time compares (example)
Group number of cases is less than 60 days 60 ~ 120 days and is greater than 120 days
Treatment group 60 17 18 25
Matched group 60 9 13 38
Table 7 result shows: there are significant difference (P<0.05) two groups of onset times, show that treatment group onset time is early than matched group.
A table 8 liang group distinct methods treatment androgenetic simultaneous phenomenon scoring is compared
The greasy scalp desquamation scalp itch of group number of cases scalp
Treatment group treatment front 60 6.63 ± 1.47 5.89 ± 1.02 5.85 ± 0.14
Treatment rear 60 2.28 ± 0.56 2.62 ± 0.36 2.68 ± 0.54
Before treatment of control group 60 6.68 ± 1.52 5.74 ± 1.53 4.97 ± 0.69
Treatment rear 60 3.46 ± 0.17 3.87 ± 0.15 3.23 ± 1.16
Table 8 result shows: two groups of simultaneous phenomenons are greasy at scalp, scalp desquamation, scalp itch comparing difference have highly significant (P<0.05).
5, untoward reaction: treatment group no side effects; Matched group has 5 examples to show as scalp itch burning sensation over the course for the treatment of, but does not affect local hair growth, does not also affect treatment, can recover gradually after stopping treatment.
With Tibetan medicine embodiment 1 tincture local application of the present invention massage, by the effect of physics and chemistry aspect, scalp microcirculation can be improved, increase blood flow, promote hair metabolism, hairy root nourishing, thus the effect reaching hair growth accelerating.Thorn is buckled in percussopunctator local, can impel neural excitation, cause vasodilation, make hair follicle vascular plexus and the hair papilla congestion of blood vessel, blood supply is abundant, is beneficial to hair growth, and button thorn is hemorrhage, poison can be rushed down eliminating evil, promoting blood circulation to remove obstruction in the collateral, makes the hair follicle vasodilation of local skin, improves local blood circulation, help medicine to absorb better, give full play to property of medicine effect.All method phases 5, energy dredging the meridian, nourishing blood to tranquillize the mind, improves natural on-off cycles of hair growth environment, accelerates the growth of hair.
This result of study shows: the cure rate for the treatment of group is 40.00%, and obvious effective rate is 31.67%, and total effective rate is 71.67%, and the cure rate of matched group is 31.67%, and obvious effective rate is 23.33%, and total effective rate is 55.00%.All there is not any untoward reaction in two groups of patients.Therefore, the clinical efficacy of Tibetan medicine embodiment 1 tincture hair growth of the present invention is definite, and safety is high.
The test example of Tibetan medicine embodiment 1 tincture of the present invention demonstrates has good effect in hair growth, also just describes the above-mentioned embodiment 1-8 enumerated and has same effect.Illustrate that Tibetan medicine of the present invention has good effect in hair growth.
Claims (4)
1. the preparation method of a hair growth Tibetan medicine, it is characterized in that comprising the following steps: that this Tibetan medicine is made up of active component or is made up of active component and pharmaceutically acceptable adjuvant, wherein said active component is made up of the crude drug of following concrete weight proportion: Cornu Bubali 10 ~ 30g, walnut shell 15 ~ 45g, Radix rumicis acetosae 7.5 ~ 22.5g, Semen Sesami Nigrum 7.5 ~ 22.5g, Caulis et Folium Lini 10 ~ 30g, Radix pedicularis muscicolae 15 ~ 45g; Mixing, adopts comminuting method or decoction and alcohol sedimentation technique or ethanol extract from water precipitation to make active component.
2. the preparation method of Tibetan medicine according to claim 1, wherein takes the crude drug of following concrete weight proportion: Cornu Bubali 16 ~ 24g, walnut shell 24 ~ 36g, Radix rumicis acetosae 12 ~ 18g, Semen Sesami Nigrum 12 ~ 18g, Caulis et Folium Lini 16 ~ 24g, Radix pedicularis muscicolae 24 ~ 36g.
3. the preparation method of Tibetan medicine according to claim 2, wherein takes the crude drug of following concrete weight: Cornu Bubali 20g, walnut shell 30g, Radix rumicis acetosae 15g, Semen Sesami Nigrum 15g, Caulis et Folium Lini 20g, Radix pedicularis muscicolae 30g.
4., according to the preparation method of any one Tibetan medicine described in claim 1-3, it also comprises the following steps: active component and pharmaceutically acceptable adjuvant to make tincture, fluid extract, extractum, ointment, liniment, lotion, aerosol or spray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410576016.7A CN104274584B (en) | 2014-10-26 | 2014-10-26 | A kind of preparation method of hair growth Tibetan medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410576016.7A CN104274584B (en) | 2014-10-26 | 2014-10-26 | A kind of preparation method of hair growth Tibetan medicine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104274584A true CN104274584A (en) | 2015-01-14 |
| CN104274584B CN104274584B (en) | 2016-06-29 |
Family
ID=52250245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410576016.7A Active CN104274584B (en) | 2014-10-26 | 2014-10-26 | A kind of preparation method of hair growth Tibetan medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104274584B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110101776A (en) * | 2019-06-25 | 2019-08-09 | 西藏圣雪生物科技有限公司 | A kind of Tibetan medicinal composition of hair-growing and anti-hair loss and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080090375A (en) * | 2008-09-19 | 2008-10-08 | 권선영 | Supernaturalanion,itchyremoval,dandruffremoval,dehairingprevention, a hair speeding up restorer |
| CN103751754A (en) * | 2014-01-28 | 2014-04-30 | 梁西鹏 | Hair restoring and blacking liquor and production method thereof |
-
2014
- 2014-10-26 CN CN201410576016.7A patent/CN104274584B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080090375A (en) * | 2008-09-19 | 2008-10-08 | 권선영 | Supernaturalanion,itchyremoval,dandruffremoval,dehairingprevention, a hair speeding up restorer |
| CN103751754A (en) * | 2014-01-28 | 2014-04-30 | 梁西鹏 | Hair restoring and blacking liquor and production method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110101776A (en) * | 2019-06-25 | 2019-08-09 | 西藏圣雪生物科技有限公司 | A kind of Tibetan medicinal composition of hair-growing and anti-hair loss and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104274584B (en) | 2016-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103169928B (en) | Externally applied traditional Chinese medicine composition for treating dysmenorrhea, as well as preparation method and application thereof | |
| CN101804185B (en) | Chinese medicinal compound extract for whitening and removing freckles and preparation method thereof | |
| CN102049008A (en) | Traditional Chinese medicine composition for treating chloasma and preparation method thereof | |
| CN108743729A (en) | A kind of herb composition that treating alopecia seborrheica and nanometer hair nourishing liquid | |
| CN104208153B (en) | One kind treats dermopathic Chinese medicine composition and its preparation | |
| CN102526326B (en) | Traditional Chinese medicine for treating postmenopausal osteoporosis and preparation method thereof | |
| US11129863B2 (en) | Composition, preparation method thereof, and application thereof in the prevention and treatment of mammary gland disease | |
| CN108969642A (en) | A kind of Chinese medicine compound prescription external preparation that treating chronic prostatitis and its preparation process | |
| CN105055849A (en) | Traditional Chinese medicine extract capable of nourishing Yin and tonifying kidney as well as preparation method and application of traditional Chinese medicine extract | |
| CN106667852A (en) | Traditional Chinese medicine cream for removing acne and preparation method thereof | |
| CN106667850A (en) | Traditional Chinese medicine mask liquid for removing acne and preparation method thereof | |
| CN106474447A (en) | A kind of antagonism head infection, compositionss of antipruritic anticreep and its preparation method and application | |
| CN101336970A (en) | Chinese herbal medicine for treating and preventing poliosis and alopecia | |
| CN104274584B (en) | A kind of preparation method of hair growth Tibetan medicine | |
| CN102048913B (en) | Traditional Chinese medicine preparation for treating climacteric syndrome and preparation method thereof | |
| CN108403932A (en) | A kind of Chinese medicine compound prescription external preparation and its preparation method and application for treating steroid dependent dermatitis | |
| CN102784216B (en) | Traditional Tibetan medicine for treating alopecia | |
| CN108403869B (en) | Traditional Chinese medicine composition for removing chloasma and preparation method thereof | |
| CN104288319B (en) | A kind of Tibetan medicine of hair growth | |
| CN107375613A (en) | A kind of Chinese medical extract and its external preparation that red blood trace on face effect is removed with Eradicates | |
| CN102793771B (en) | Preparation method of Tibetan drug for treating alopecia | |
| CN104288320B (en) | A kind of Tibetan medicine of hair growth | |
| CN104274585B (en) | A kind of preparation method of hair growth Tibetan medicine | |
| CN100409885C (en) | Medicine for treating proganital lichen sclerosus disease | |
| CN104042944A (en) | Medicinal preparation used for treating chloasma |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |