CN104259193B - A kind of method repairing atrazine pesticide contaminated soil - Google Patents
A kind of method repairing atrazine pesticide contaminated soil Download PDFInfo
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- CN104259193B CN104259193B CN201410497863.4A CN201410497863A CN104259193B CN 104259193 B CN104259193 B CN 104259193B CN 201410497863 A CN201410497863 A CN 201410497863A CN 104259193 B CN104259193 B CN 104259193B
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- atrazine
- contaminated soil
- pesticide contaminated
- soil
- metallurgical slag
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- MXWJVTOOROXGIU-UHFFFAOYSA-N Atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 title claims abstract description 77
- 239000002689 soil Substances 0.000 title claims abstract description 77
- 239000000575 pesticide Substances 0.000 title claims abstract description 51
- 239000002893 slag Substances 0.000 claims abstract description 34
- 210000004215 spores Anatomy 0.000 claims abstract description 31
- 239000000203 mixture Substances 0.000 claims abstract description 24
- 239000000725 suspension Substances 0.000 claims abstract description 23
- 241000233866 Fungi Species 0.000 claims abstract description 15
- 239000000126 substance Substances 0.000 claims abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 235000009566 rice Nutrition 0.000 claims abstract description 6
- 239000010902 straw Substances 0.000 claims abstract description 6
- 241000222393 Phanerochaete chrysosporium Species 0.000 claims description 12
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium monoxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 12
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 12
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 12
- VASIZKWUTCETSD-UHFFFAOYSA-N manganese(II) oxide Inorganic materials [Mn]=O VASIZKWUTCETSD-UHFFFAOYSA-N 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 241000209094 Oryza Species 0.000 claims description 5
- 230000015556 catabolic process Effects 0.000 claims description 3
- 229910052681 coesite Inorganic materials 0.000 claims description 3
- 229910052906 cristobalite Inorganic materials 0.000 claims description 3
- 230000004059 degradation Effects 0.000 claims description 3
- 238000006731 degradation reaction Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 229910052904 quartz Inorganic materials 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 229910052682 stishovite Inorganic materials 0.000 claims description 3
- 229910052905 tridymite Inorganic materials 0.000 claims description 3
- 210000000038 chest Anatomy 0.000 claims description 2
- 235000019525 fullness Nutrition 0.000 claims description 2
- 230000035935 pregnancy Effects 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000011109 contamination Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 10
- 239000003814 drug Substances 0.000 abstract description 4
- 229940079593 drugs Drugs 0.000 abstract description 4
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 230000037250 Clearance Effects 0.000 description 21
- 230000035512 clearance Effects 0.000 description 21
- 238000000034 method Methods 0.000 description 12
- 238000007792 addition Methods 0.000 description 11
- 244000005700 microbiome Species 0.000 description 8
- 230000003647 oxidation Effects 0.000 description 8
- 238000007254 oxidation reaction Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000008187 granular material Substances 0.000 description 6
- 238000005067 remediation Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- LIVNPJMFVYWSIS-UHFFFAOYSA-N silicon monoxide Inorganic materials [Si-]#[O+] LIVNPJMFVYWSIS-UHFFFAOYSA-N 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000001488 breeding Effects 0.000 description 2
- 210000004027 cells Anatomy 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth media Substances 0.000 description 2
- 230000002363 herbicidal Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006011 modification reaction Methods 0.000 description 2
- 238000005429 turbidity Methods 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N AI2O3 Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 241001465754 Metazoa Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 238000003723 Smelting Methods 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 231100000704 bioconcentration Toxicity 0.000 description 1
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- 229910052593 corundum Inorganic materials 0.000 description 1
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- 239000008121 dextrose Substances 0.000 description 1
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- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 229960002163 hydrogen peroxide Drugs 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 150000002505 iron Chemical class 0.000 description 1
- -1 it addition Substances 0.000 description 1
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Abstract
The invention discloses a kind of method repairing atrazine pesticide contaminated soil, comprise the following steps: metallurgical slag and atrazine pesticide contaminated soil are mixed to get mixture, in mixture, add H2O2Solution, carries out fenton-type reagent degraded and obtains catabolite；Catabolite is mixed with rice straw powder, whiterot fungi spore suspension, has been degraded the reparation to atrazine pesticide contaminated soil by biofermentation.The present invention uses the atrazine pesticide in metallurgical slag and whiterot fungi combined removal soil, has cost relatively low, and treatment effeciency is high, high treating effect, and the chemical drugs dosage added is few, it is possible to effectively improve the advantages such as soil property.
Description
Technical field
The present invention relates to field of environmental improvement, particularly relate to a kind of method repairing atrazine pesticide contaminated soil.
Background technology
Pesticide, since invention, at preventing and treating corps diseases, insect pest, crop smothering, it is ensured that crops normal growth, has ensured farming
Thing high yield and bumper harvest, solves mankind's food problem aspect and has played highly important effect.Atrazine has another name called G-30027, English name
Claiming atrazine, chemical name is 2-chloro-4-ethylamino--6-isopropylamine base-1,3,5-tri-pyridines, puts into business in nineteen fifty-nine raw
Produce, within about 1980, start to use in China.Atrazine low price and have good herbicidal effect, becomes the most complete
One of the most widely used herbicide of ball.But atrazine stable in properties is the most degradable, and has bioconcentration, to people
Class and animals and plants have serious harm, and while life-time service the most again, the pesticide residues of accumulation have become as a grand duke
Evil.Therefore, the reparation of contaminated soil can be suppressed from source the diffusion of pollution by pesticides, it addition, pesticide producing base, storage
The contaminated site stayed after the resettlement such as warehouse is also required to repair.
Chemical oxidation method can remove the organic pollution in soil effectively, but is intended to reach higher clearance,
Generally require and add substantial amounts of chemical drugs, not only the character of soil can be damaged but also Financial cost is higher.Biology is repaiied
Recovering technology is the new technique that environmental area develops rapidly in recent years, and it is with the dirt in the degraded of various organisms or absorption environment
Dye thing.Wherein, Phanerochaete chrysosporium (Phanerochaete chrysosporium), can be with wooden fibre in nature
Dimension element carries out growth and breeding as nutrient, and its extracellular enzyme produced can non-specifically be degraded a series of dirt in environment
Dye thing.Compared with traditional method, biological method has the advantages such as energy consumption is low, simple to operate, viable economically, but also has target
The shortcomings such as microorganism and indigenous microbes are competed, time-consuming length.Therefore, by biologic treating technique and the advantage of chemical oxidation techniques
Connected applications has great importance in atrazine-containing pesticide contaminated soil reparation.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, it is provided that a kind for the treatment of effeciency is high, process effect
The best, cost is relatively low and the reparation atrazine-containing pesticide contaminated soil method of environmental protection.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
Provide a kind of method repairing atrazine pesticide contaminated soil, comprise the following steps:
S1, metallurgical slag and atrazine pesticide contaminated soil are mixed to get mixture, in aforementioned mixture, add H2O2
Solution, carries out fenton-type reagent degraded and obtains catabolite；
S2, catabolite abovementioned steps S1 prepared mix with rice straw powder, whiterot fungi spore suspension, logical
Cross biofermentation to have degraded the reparation to atrazine pesticide contaminated soil.
Further, aforementioned H in aforementioned S1 step2O2Solution divides 3 times and joins in aforementioned mixture, each interval time
For 4h.
Further, in aforementioned S1 step, aforementioned metallurgical slag includes CaO, 22～the 37wt%'s of 26～40wt%
SiO2, 3～the Fe of 18wt%2O3, 1～the Al of the MgO of the FeO of 13wt%, 0.3～8wt%, 0.1～5wt%2O3, 0.1～
The P of 3wt%2O5, 0.1～the MnO of 1.4wt%.
Further, metallurgical slag includes the CaO of 38.2wt%, the SiO of 26.4wt%2, the Fe of 16.3wt%2O3,
The Al of the MgO of the FeO of 8.2wt%, 6.1wt%, 2.7wt%2O3, the P of 1.4wt%2O5, the MnO of 0.4wt%.
Further, aforementioned whiterot fungi spore suspension is preferably the spore suspension of Phanerochaete chrysosporium, every mL spore
Fullness over the chest during pregnancy supernatant liquid contains spore 2.0 × 106Individual.
Further, in aforementioned S1 step the mass ratio of metallurgical slag and atrazine pesticide contaminated soil be 0.05: 1～
0.1: 1, H2O2The volume mass ratio 0.05～0.15L: 1kg of solution and atrazine pesticide contaminated soil, aforementioned H2O2The matter of solution
Amount mark is 30%.
Further, aforementioned rice straw powder and the mass ratio 0.05 of aforementioned atrazine pesticide contaminated soil in aforementioned S2 step:
1。
Further, aforementioned whiterot fungi spore suspension and the body of aforementioned atrazine pesticide contaminated soil in aforementioned S2 step
Long-pending mass ratio 20mL～50mL: 1kg.
Further, in aforementioned atrazine pesticide contaminated soil, the concentration of atrazine is 0～600mg/kg, preferred concentration
It is 0～300mg/kg.
Further, the time of aforementioned biological fermentative degradation is 12～30d, and preferred degradation time is 26～30d.
The creativeness of the present invention is:
The present invention uses metallurgical slag and whiterot fungi synergy to repair atrazine pesticide contaminated soil, due to metallurgical slag
Iron salt content is higher, adds hydrogenperoxide steam generator and Fenton or embryonic stem-like cells can be occurred to produce the hydroxyl that oxidisability is extremely strong
Organic substance is aoxidized by free radical (OH), and its concrete reaction equation is:
Fe3++H2O2→Fe2++H++HO2,
Adding whiterot fungi in the atrazine pesticide contaminated soil of embryonic stem-like cells, whiterot fungi can secrete exoenzyme
Atrazine pesticide contaminated soil is degraded further；Meanwhile, the Ca contained by slag, the element such as Mg, Mn also can promote white rot
The growth and breeding of bacterium, further increases the whiterot fungi removal ability to atrazine-contaminated soil.
Compared with prior art, it is an advantage of the current invention that:
1, the present invention uses chemical oxidation to combine bioremediation technology and repairs atrazine-contaminated soil, has chemical agent and throws
Enter less, the advantage such as environmental protection, there is complementary effect simultaneously, compared to individually using chemistry between metallurgical slag and whiterot fungi
Oxidation processes or bioremediation technology, the method for the present invention is higher to the clearance of atrazine in contaminated soil.
2, the rice straw powder added in the restorative procedure of the present invention both can be that Phanerochaete chrysosporium provides growth substrate, again
The character of contaminated soil can be improved, as increased hole between soil particle, the humus formed after Phanerochaete chrysosporium is degraded
Also improve the fertility of soil.
3, the restorative procedure of the present invention achieves the refuse reclamation of metallurgical slag, relative to traditional chemical cost of drugs relatively
Low, treatment effeciency is high, high treating effect, and the chemical drugs dosage added is few, it is possible to effectively improve soil property.
Detailed description of the invention
Below in conjunction with concrete preferred embodiment, the invention will be further described, but not thereby limiting the invention
Protection domain.
Raw material and instrument employed in following example are commercially available.Wherein metallurgical slag is purchased from zhuzhou,hunan smelting
Factory, its main component includes being the SiO of CaO, 22～the 37wt% of 26～40wt%2, 3～the Fe of 18wt%2O3, 1～13wt%
The Al of the MgO of FeO, 0.3～8wt%, 0.1～5wt%2O3, 0.1～the P of 3wt%2O5, 0.1～the MnO of 1.4wt%.Bag
Metallurgical slag containing mentioned component all can apply in the method for the present invention, and in the embodiment of the present invention, preferred metallurgical slag contains
The SiO of the CaO of 38.2wt%, 26.4wt%2, the Fe of 16.3wt%2O3, the MgO of the FeO of 8.2wt%, 6.1wt%, 2.7wt%
Al2O3, the P of 1.4wt%2O5, the MnO of 0.4wt%.
Before using above-mentioned metallurgical slag, first metallurgical slag is broken into after drying the granule of diameter about 1mm, then
Process for atrazine pesticide contaminated soil.
The whiterot fungi spore suspension of the present invention refers to Phanerochaete chrysosporium (Phanerochaete
Chrysosporium, CCTCC AF96007) spore suspension, but the present invention is not limited in Phanerochaete chrysosporium spore
Suspension.This suspension is to be prepared by the following method: the Rhizoma Solani tuber osi glucose training being placed in by strain in the biochemical cultivation case of 37 DEG C
Supporting and cultivate on base, after 6d, the preferable strain of growth selection situation carries out preservation in being placed on the refrigerator of 4 DEG C；At sterilizing platform
In, dissolve in sterilized water with aseptic cotton carrier viscous spore taking object bacteria from culture medium, make spore uniform by stirring and vibrations
Dispersion, forms spore suspension.Measuring the turbidity of solution, when turbidity is about 60%, the yellow archespore hair used by the present invention is put down
The spore suspension of lead fungi.Described Rhizoma Solani tuber osi dextrose culture-medium consists of: Rhizoma Solani tuber osi 40g, glucose 4g, water 200mL.
The preparation method of Caulis et Folium Oryzae granule is: cleaned by the Caulis et Folium Oryzae Radix Platycodonis clear water collected from field, after natural air drying, uses
Mill passes through 5mm nylon sieve after grinding, and collects standby.
Atrazine-contaminated soil of the present invention takes from Yue Lu mountain, Changsha, Hunan, one provided by the present invention
The method repairing atrazine pesticide contaminated soil can be applicable to the atrazine pesticide contaminated soil of any character, all can reach preferable
Technique effect.
Embodiment 1
Take atrazine content and be respectively 100, the contaminated soil of 200,300,400,500,600mg/kg, use following steps
Process:
(1) metallurgical slag the mix homogeneously that add 0.05kg in the atrazine pesticide contaminated soil of 1kg are mixed
Thing；Adding 0.05L mass fraction every 4h in mixture is the H of 30%2O2Solution, adds 3 times altogether, by interpolation go from
It is about 30% that sub-water adjusts system moisture content, carries out fenton-type reagent reaction 24h.
(2) add in the atrazine pesticide contaminated soil of fenton-type reagent the Caulis et Folium Oryzae granule of 0.05kg with
(every mL spore suspension contains spore 2.0 × 10 to the Phanerochaete chrysosporium spore suspension of 0.05L6Individual) and mix homogeneously, Gu
State fermentation 30d, completes the reparation to pesticide contaminated soil.
Detected by extraction-high performance liquid chromatography from the sampling of each individual system after oxidation 24h and microorganism remediation 30d
The content of atrazine in soil, measurement result is shown in Table 1.
Table 1: atrazine clearance testing result table in atrazine pesticide contaminated soil
| Atrazine content (mg/kg) | 100 | 200 | 300 | 400 | 500 | 600 |
| 24h clearance (%) | 91.25 | 88.16 | 61.74 | 47.22 | 39.67 | 33.41 |
| 30d clearance (%) | 97.48 | 97.12 | 96.15 | 82.63 | 71.21 | 59.29 |
As shown in Table 1, this restorative procedure is to the pesticide contaminated soil that atrazine content is 100mg/kg～600mg/kg
Repairing effect is notable, wherein atrazine content be 100mg/kg～300mg/kg pesticide contaminated soil clearance all 95% with
On.Visible, utilize the method for the present invention to repair the pesticide contaminated soil of atrazine-containing concentration≤300mg/kg, effect is best.
Embodiment 2
(1) 1kg atrazine pesticide contaminated soil (in atrazine pesticide contaminated soil, atrazine content is 300mg/kg)
In be separately added into the metallurgical slag of 0.05kg and mix homogeneously obtains mixture；0.05L mass is added every 4h in mixture
Mark is the H of 30%2O2Solution, adds 3 times altogether, being about 30% by add deionized water adjusting system moisture content, carrying out
Fenton-type reagent reaction 24h；
(2) take 5 parts of atrazine pesticide contaminated soils through fenton-type reagent, add the Caulis et Folium Oryzae granule of 0.05kg respectively
(every mL spore suspension contains spore 2.0 × 10 with the Phanerochaete chrysosporium spore suspension of 0.05L6Individual) and mix homogeneously,
Solid fermentation 30d, completes the reparation to pesticide contaminated soil.
Sample by the content of atrazine in extraction-high performance liquid chromatography detection soil during microorganism remediation,
Measurement result is shown in Table 2.
The clearance testing result table of atrazine during table 2 microorganism remediation
| Time (d) | 12 | 14 | 16 | 18 | 20 | 22 | 24 | 26 | 28 | 30 |
| Clearance (%) | 79.45 | 84.45 | 88.04 | 91.39 | 93.03 | 93.87 | 94.41 | 95.04 | 95.12 | 95.18 |
As shown in Table 2, in 26d, soil the removal efficiency of atrazine more than 95%.Owing to processing effect with 24d after 26d
Really difference is little, so from the standpoint of time-consuming, the inventive method is repaired the optimal biology of atrazine-contaminated soil and repaiied
The multiple time is 26d.
Embodiment 3
Take 5kg atrazine pesticide contaminated soil (in atrazine pesticide contaminated soil, atrazine content is 300mg/kg), will
It is equally divided into 5 parts, every part of 1kg:
(1) in the contaminated soil of 5 parts of 1kg, be separately added into 0,0.05,0.1,0.15, the metallurgical slag of 0.2kg mixing
Uniformly obtain mixture；
(2) adding 0.05L mass fraction every 4h in 5 parts of mixture is the H of 30%2O2Solution, adds 3 times altogether, logical
Crossing interpolation deionized water and adjusting system moisture content is about 30%, carries out fenton-type reagent 24h；
(3) in 5 parts of treated contaminated soils, add the Caulis et Folium Oryzae granule of 0.05kg and yellow archespore Mao Pingge of 0.05L
(every mL spore suspension contains spore 2.0 × 10 to bacterium spore suspension6Individual) and mix homogeneously, solid fermentation 30d, complete pesticide
The reparation of contaminated soil.
Detected by extraction-high performance liquid chromatography from the sampling of each individual system after oxidation 24h and microorganism remediation 26d
The content of atrazine in soil, measurement result is shown in Table 3.
Table 3: the clearance result table of atrazine under different slag additions
| Slag (kg) | 0 | 0.05 | 0.1 | 0.15 | 0.2 |
| 24h clearance (%) | 26.23 | 62.18 | 65.73 | 47.24 | 45.61 |
| 26d clearance (%) | 57.49 | 96.06 | 97.25 | 73.41 | 53.78 |
From table 3, slag addition increases to 0.05kg/kg from 0kg/kg, the clearance of atrazine 24h from
26.23% increases to 62.18%, increases the addition of slag further, and the clearance of atrazine slightly improves, when adding of slag
Dosage is when more than 0.15kg/kg, and the clearance of atrazine the most significantly drops to less than 50%.From going of atrazine 26d
From the point of view of rate, when the addition of slag is 0.1kg/kg, the atrazine of 97.25% is had to be removed, it is seen that the suitable interpolation of slag
Amount is 0.05kg/kg～0.1kg/kg, and optimum addition is 0.1kg/kg.It is unnecessary that the metallurgical slag of excess not only can occur
Consumption of chemical reaction H2O2, the pH simultaneously also resulting in soil is too high, is unfavorable for the growth of microorganism.Therefore the adding of metallurgical slag
The clearance of atrazine pesticide contaminated soil is affected notable by dosage.
Embodiment 4
Take 4kg atrazine pesticide contaminated soil (in atrazine pesticide contaminated soil, atrazine content is 400mg/kg), will
Atrazine pesticide contaminated soil is equally divided into 4 parts, every part of 1kg:
(1) in the contaminated soil of 4 parts of 1kg, each metallurgical slag adding 0.1kg mix homogeneously obtain mixture；
(2) in 4 parts of mixture, be separately added into 0.05 every 4h, 0.075,0.1,0.0125,0.15L mass fraction be
The H of 30%2O2Solution, adds 3 times altogether, being about 30% by add deionized water adjusting system moisture content, carrying out class
Fenton oxidation is angry answers 24h；
(3) in 4 parts of treated contaminated soils, add the Caulis et Folium Oryzae granule of 0.05kg and yellow archespore Mao Pingge of 0.05L
Bacterium spore suspension mix homogeneously, solid fermentation 26d, complete the reparation to pesticide contaminated soil.
Detected by extraction-high performance liquid chromatography from the sampling of each individual system after oxidation 24h and microorganism remediation 26d
The content of atrazine in soil, measurement result is shown in Table 4.
The different H of table 42O2The clearance of atrazine under solution addition
| H2O2Solution (L) | 0.05 | 0.075 | 0.1 | 0.125 | 0.15 |
| 24h clearance (%) | 49.83 | 65.37 | 74.16 | 77.51 | 79.73 |
| 26d clearance (%) | 83.62 | 94.58 | 95.35 | 95.41 | 95.22 |
From table 4, at H2O2Each addition of solution is 0.05～0.15L/kg, and the clearance of atrazine is higher, when
When addition is 0.1L/kg, atrazine 26d clearance reaches 95.35%, illustrates by increasing H2O2The addition of solution, this
Bright method can apply to repair the contaminated soil of higher atrazine content.
Therefore, the repairing effect utilizing the method reparation atrazine-containing soil of the present invention is good, adds at metallurgical slag
Amount is 0.1kg/kg, H2O2Solution addition be each 0.05L/kg, under conditions of microorganism remediation 26d, the inventive method
The clearance of the atrazine that content in soil is 300mg/kg be can reach 96.52%.By increasing H2O2Solution interpolation
Amount, the inventive method can repair the atrazine contaminated soil of higher amount.
In the embodiment above, the addition of Phanerochaete chrysosporium spore suspension all can reach at 0.02L～0.05L
Same or analogous technique effect.
The above, be only presently preferred embodiments of the present invention, and the present invention not makees any pro forma restriction.Though
So the present invention discloses as above with preferred embodiment, but is not limited to the present invention.Any it is familiar with those skilled in the art
Member, in the case of without departing from the spirit of the present invention and technical scheme, may utilize in method and the technology of the disclosure above
Hold and technical solution of the present invention is made many possible variations and modification, or be revised as the Equivalent embodiments of equivalent variations.Therefore,
Every content without departing from technical solution of the present invention, according to the present invention technical spirit to made for any of the above embodiments any simply
Amendment, equivalent, equivalence change and modification, all still fall within the range of technical solution of the present invention protection.
Claims (8)
1. the method repairing atrazine pesticide contaminated soil, it is characterised in that comprise the following steps:
S1, metallurgical slag and atrazine pesticide contaminated soil are mixed to get mixture, in described mixture, add H2O2Molten
Liquid, carries out fenton-type reagent degraded and obtains catabolite；Described metallurgical slag includes CaO, 22～the 37wt% of 16～40wt%
SiO2, 3～the Fe of 18wt%2O3, 1～the Al of the MgO of the FeO of 13wt%, 0.3～8wt%, 0.1～5wt%2O3, 0.1～3wt%
P2O5, 0.1～the MnO of 1.4wt%；
S2, the catabolite described step S1 prepared mix with rice straw powder, whiterot fungi spore suspension, by life
Thing fermentative degradation completes the reparation to atrazine pesticide contaminated soil；Metallurgical slag described in described step S1, atrazine pesticide
Contaminated soil and H2O2The mass volume ratio of solution is (0.05～0.1 kg): 1 kg: (0.05～0.15L).
Method the most according to claim 1, it is characterised in that H described in described S1 step2O2Solution divides 3 times and joins institute
Stating in mixture, each interval time is 4h.
Method the most according to claim 1, it is characterised in that described metallurgical slag includes the CaO of 38.2wt%, 26.4wt%
SiO2, the Fe of 16.3wt%2O3, the Al of the MgO of the FeO of 8.2wt%, 6.1wt%, 2.7wt%2O3, the P of 1.4wt%2O5,
The MnO of 0.4wt%.
Method the most according to claim 1, it is characterised in that described whiterot fungi spore suspension is Phanerochaete chrysosporium
Spore suspension, every mL spore suspension contain spore 2.0 × 106Individual.
Method the most according to any one of claim 1 to 4, it is characterised in that atrazine agriculture described in described step S1
In chemicals contamination soil, the concentration of atrazine is 0～600mg/kg.
Method the most according to any one of claim 1 to 4, it is characterised in that rice straw powder described in described step S2 with
The mass ratio 0.05: 1 of described atrazine pesticide contaminated soil.
Method the most according to any one of claim 1 to 4, it is characterised in that whiterot fungi spore described in described step S2
The volume mass of fullness over the chest during pregnancy supernatant liquid and described atrazine pesticide contaminated soil is than 20mL～50mL: 1kg.
Method the most according to any one of claim 1 to 4, it is characterised in that biofermentation degraded in described step S2
Time be 12～30d.
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| CN201410497863.4A CN104259193B (en) | 2014-09-25 | A kind of method repairing atrazine pesticide contaminated soil |
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| CN201410497863.4A CN104259193B (en) | 2014-09-25 | A kind of method repairing atrazine pesticide contaminated soil |
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| US6423531B1 (en) * | 1999-11-17 | 2002-07-23 | Geovation Technologies, Inc. | Advanced organic-inorganic solid-chemical composition and methods for anaerobic bioremediation |
| CN101306427A (en) * | 2007-05-17 | 2008-11-19 | 中国科学院生态环境研究中心 | Method for increasing organic pollutant degradation efficiency in soil |
| CN103240267A (en) * | 2013-04-28 | 2013-08-14 | 华中师范大学 | Soil restoration method based on oxygen activation of iron powder and polyphosphoric acid molecules |
| CN103882003A (en) * | 2014-03-11 | 2014-06-25 | 东北农业大学 | Bacterial strain composite carrier material for restoration of atrazine polluted soil as well as preparation method and application thereof |
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