CN104258456A - Wound repair gel containing hexagonal mesoporous silicon and preparation method thereof - Google Patents
Wound repair gel containing hexagonal mesoporous silicon and preparation method thereof Download PDFInfo
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- CN104258456A CN104258456A CN201410438877.9A CN201410438877A CN104258456A CN 104258456 A CN104258456 A CN 104258456A CN 201410438877 A CN201410438877 A CN 201410438877A CN 104258456 A CN104258456 A CN 104258456A
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- mesoporous silicon
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Abstract
The invention discloses a wound repair gel containing hexagonal mesoporous silicon and a preparation method thereof. The wound repair gel containing hexagonal mesoporous silicon comprises the components including hexagonal mesoporous silicon, basic fibroblast growth factor FGF-21, chitosan, a surfactant, a cosurfactant, glycerin monostearate, Carbomer, a pH adjustor and deionized water. According to the invention, active substances and effective components are loaded by utilizing mesoporous silicon, so that the stability can be increased, and the slow-release effect can be realized; the growth factors can stimulate cell locomotion, cell merisis and synthesis of cell meso-protein, promote skin blood capillary generation and tissue growth, and benefit scar repair; chitosan has the inhibition effect to several kinds of bacteria, also has good film-forming property, and is beneficial to wound healing and bleeding stopping; simultaneously, glycerin monostearate provided in the wound repair gel also has the effect of moisturizing skin.
Description
Technical field
The invention belongs to medical art, be specifically related to a kind of wound repair gel containing hexagonal mesoporous silicon and preparation method thereof.
Background technology
Skin is made up of epidermis, corium and subcutaneous tissue, and its function is that protective inner tissue is damaged from the external world; Carry out metabolism such as absorption, perspire, sebum secreted and row and rush down refuse etc.; Skin can also regulate body temperature, impression pain, touch, pressure, the stimulation such as warm, cold, and have immunization.People is inevitably subject to the different wound of various weight in life, asks that it is wound, natural disaster and ill rear operation wound often can hardly be avoided.When skin injury acquires a certain degree, due to fibroproliferative reaction, skin ultrastructure will come to an end with cicatrization.Various body surface cicatrix all can destroy original skin integrity, damages appearance, brings the injury of body and mind to patient.While repair in trauma, how to treat its appearance destruction of causing is one of important subject of medical domain always.
The Developing Application of current new pattern compress, skin transplantation, gene therapy, cultured cell transplants and artificial skin is transplanted, new technology has all appearred in the application of cell growth factor and TCM Therapy.Traditional dressing can keep the drying property that wound surface is good, but can not effectively stop wound surface infected, and easily and planing surface stick together, wound surface may be caused during removal again impaired; Novel synthesis type dressing has good elasticity and breathability, but hygroscopicity is bad, and wound surface place may be caused to form hydrops and cause the breeding of antibacterial.Be applied to clinical tissue engineering product and there is certain repulsion immunoreation, therapeutic effect is not good enough.
So far, in repair in trauma material, add that somatomedin is existing to be reported.Chinese patent 201110053558.2 discloses collagen protein slow releasing carrier material of the composite growth factor that a kind of short oral cavity forehead various wounds is repaired and preparation method thereof; Chinese patent 200510022581.X discloses biological sponge material for promoting injury-restoring and preparation method thereof, wherein in every gram of biological activity sponge material containing the somatomedin being no less than 10ng; Chinese patent 200710048698.4 discloses a kind ofly has the preparation method organizing epigamic collagen matrices surface wound repairing membrane, introduces the repair membrane material of the gelatin-based sustained-release micro-spheres containing cell growth factor, has good degradability and biocompatibility.But, hexagonal mesoporous silicon is made gelatinous wound repair material have not been reported as the slow-released carrier of active substance.Due to the special structure and morphology of hexagonal mesoporous silicon, hole dimension, excellent biocompatibility and safety, the stability and slow releasing function that improve active matter can be played, and to no skin irritation, have no side effect.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide one can promote skin surface repair in trauma fast, and there is wound repair gel containing hexagonal mesoporous silicon of slow release effect and preparation method thereof.
Containing a preparation method for the wound repair gel of hexagonal mesoporous silicon, comprise the following steps:
After basic fibroblast growth factor FGF-21, chitosan, hexagonal mesoporous silicon and glyceryl monostearate mix homogeneously, add surfactant and cosurfactant, slow stirring 3 ~ 5min, then dropwise deionized water is dripped, after abundant mixing, with high-shear homogenizer homogenizing 10 ~ 15min under 6000 ~ 8000rpm speed, obtain the emulsion containing mesoporous silicon;
Carbomer is added to the water, leaves standstill after stirring and make that it is fully swelling, be uniformly dispersed formation semi-solid gel; The above-mentioned emulsion containing mesoporous silicon is slowly added wherein, adjust ph to 6.8 ~ 7.5, the obtained wound repair gel containing hexagonal mesoporous silicon.
In said method, 100g total amount pressed by described wound repair gel, it is composed as follows: hexagonal mesoporous silicon 0.1 ~ 1g, basic fibroblast growth factor 1 ~ 100 μ g, chitosan 100 ~ 150 μ g, surfactant 15 ~ 20g, cosurfactant 20 ~ 40g, glyceryl monostearate 2 ~ 10g, carbomer 0.5 ~ 5g, all the other are deionized water.
In said method, the preparation method of described hexagonal mesoporous silicon is: be dissolved in the beaker of 100ml by 2 ~ 5g lauryl amine, 20 ~ 60ml dehydrated alcohol, 20 ~ 60ml deionized water, use magnetic stirring apparatus rapid stirring; Then add 5 ~ 10g ethyl orthosilicate and stir ageing 30min after 8 ~ 12h; Use deionized water and washing with alcohol respectively, put into high-temperature calcination after oven drying, obtained hexagonal mesoporous Si powder.
In said method, described surfactant comprises: more than one in Tween80, sodium lauryl sulphate or polyethyleneglycol glyceride.
In said method, described cosurfactant comprises: more than one in ethanol, propylene glycol, isopropyl alcohol or glycerol.
In said method, described basic fibroblast growth factor FGF-21 is human alkaline fibroblast growth factor.
In said method, pH adjusting agent used is one or more in citric acid, malic acid, ethylenediaminetetraacetic acid or triethanolamine.
Compared with prior art, tool of the present invention has the following advantages:
(1) utilize the loose structure carrying active ingredients of mesoporous silicon, have controlled-release function to active component, obviously can reduce administration number of times.
(2) in the present invention containing basic fibroblast growth factor, can the synthesis of irritation cell merisis and intercellular protein, promote that capillary of skin generates, be conducive to tissue growth, promote wound healing, decrease the generation of cicatrix; Chitosan is inhibited to various bacteria simultaneously, also has good film property, is conducive to wound healing and hemostasis.
(3) the present invention adopts gel preparations, has certain rheological characteristic and thixotropy, smears time easy to use.
(4) contain skin moistening composition in the present invention, the effect of moistening is play to skin.
Accompanying drawing explanation
Fig. 1 is the embodiment of the present invention 1 bFGF In-vitro release curves.
Fig. 2 is the upgrowth situation of L929 cell on each group of material in the embodiment of the present invention 1.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, but the protection domain of application claims is not limited to this.
Hexagonal mesoporous silicon preparation method used by following examples is as follows: be dissolved in the beaker of 100ml by 2g lauryl amine, 20ml dehydrated alcohol, 20ml deionized water, use magnetic stirring apparatus rapid stirring; Then add 8g ethyl orthosilicate and stir ageing 30min after 8 ~ 12h; Spend 80ml ionized water and 80ml washing with alcohol respectively, then put into 640 DEG C of high-temperature calcination 4h after 80 DEG C of oven dryings, obtained hexagonal mesoporous Si powder.
Embodiment 1
5 μ g basic fibroblast growth factor FGF-21,100 μ g chitosans and 0.5g mesoporous silicon are added in 8g glyceryl monostearate and fully stirs, obtain mixed liquor; Again that 15gTween80 and 30g mixed with propylene glycol is even, add in above-mentioned mixed liquor, in the process slowly stirred, dropwise drip deionized water; With high-shear homogenizer homogenizing 15min under 6000rpm speed, obtain the emulsion containing mesoporous silicon.
Take 2g Acritamer 940 to be added to the water, leave standstill after stirring and make that it is fully swelling, be uniformly dispersed formation semi-solid gel; The above-mentioned emulsion containing mesoporous silicon is slowly added wherein, adds 0.05g triethanolamine and regulate pH to 7.2, the obtained wound repair gel containing hexagonal mesoporous silicon.
Embodiment 2
10 μ g basic fibroblast growth factor FGF-21,100 μ g chitosans and 0.5g mesoporous silicon are added in 8g glyceryl monostearate and fully stirs, obtain mixed liquor; Again by 15g Tween80 and 30g ethanol mix homogeneously, add in above-mentioned mixed liquor, in the process slowly stirred, dropwise drip deionized water; With high-shear homogenizer homogenizing 10min under 8000rpm speed, obtain the emulsion containing mesoporous silicon.
Take 2g Acritamer 940 to be added to the water, leave standstill after stirring and make that it is fully swelling, be uniformly dispersed formation semi-solid gel; The above-mentioned emulsion containing mesoporous silicon is slowly added wherein, adds 0.05g triethanolamine and regulate pH to 7.2, the obtained wound repair gel containing hexagonal mesoporous silicon.
Embodiment 3
20 μ g basic fibroblast growth factor FGF-21,150 μ g chitosans and 1g mesoporous silicon are added in 10g glyceryl monostearate and fully stirs, obtain mixed liquor; Again that 20g Tween80 and 40g mixed with propylene glycol is even, add in above-mentioned mixed liquor, in the process slowly stirred, dropwise drip deionized water; With high-shear homogenizer homogenizing 12min under 7000rpm speed, obtain the emulsion containing mesoporous silicon.
Take 5g Acritamer 940 to be added to the water, leave standstill after stirring and make that it is fully swelling, be uniformly dispersed formation semi-solid gel; The above-mentioned emulsion containing mesoporous silicon is slowly added wherein, adds 0.05g Fructus Citri Limoniae acid for adjusting pH to 6.8, the obtained wound repair gel containing hexagonal mesoporous silicon.
Embodiment 4
5 μ g basic fibroblast growth factor FGF-21,150 μ g chitosans and 1g mesoporous silicon are added in 10g glyceryl monostearate and fully stirs, obtain mixed liquor; Again by 20g Tween80 and 40g glycerol mix homogeneously, add in above-mentioned mixed liquor, in the process slowly stirred, dropwise drip deionized water; With high-shear homogenizer homogenizing 10min under 8000rpm speed, obtain the emulsion containing mesoporous silicon.
Take 5g Acritamer 940 to be added to the water, leave standstill after stirring and make that it is fully swelling, be uniformly dispersed formation semi-solid gel; The above-mentioned emulsion containing mesoporous silicon is slowly added wherein, adds 0.05g Fructus Mali pumilae acid for adjusting pH to 6.8, the obtained wound repair gel containing hexagonal mesoporous silicon.
The wound repair gel prepared with embodiment 1 is below tested, and other embodiments 2 ~ 4 test effect basic simlarity with embodiment 1, do not illustrate one by one.
One, Wound healing rate test:
Get the SD rat of 12 about 200g, anesthesia back part shaves hair, respectively do the otch of 5 millimeters deep, 3 centimeter length at back with scalpel, matched group uses normal saline, experimental group site of injury smears the wound repair gel of the embodiment of the present invention 1, respectively at 3,7,14 days records wound healing rate (table 1).Result shows, after having smeared wound repair gel of the present invention, the healing rate of wound surface significantly improves.
The different disposal group rat skin wound healing rate of table 1 different time points record
Two, Suspension quantitative bactericidal test:
Depletion Staphylococcus aureus (ATCC 6538), escherichia coli (8099), Candida albicans (ATCC10231), inclined-plane fresh cultured thing, gel of the present invention is put into sterilized petri dishes, puts into 5ml nutrient broth pipe simultaneously; The blood plate of inoculated bacteria and meat soup pipe are put 37 DEG C and cultivate 48h, observe PRELIMINARY RESULTS, continue to be cultured to the 7th day at asepsis growth pipe.If meat soup pipe muddiness and blood plate have bacteria growing, be designated as the positive, represent with (+); As the 7th day still clarifies, be considered as asepsis growth, be designated as feminine gender, with (–) represent.
The antibiotic property of wound repair gel of the present invention is negative as can be seen from Table 2, namely demonstrates good bacteriostatic activity.
Table 2 is containing the Suspension quantitative bactericidal test of the wound repair gel of hexagonal mesoporous silicon
Three, external medicine SR test:
Wound repair gel of the present invention is put into bag filter and bag filter is put into the beaker containing PBS solution, then be placed in the thermostat water bath of 37 DEG C, take out 5mlPBS solution every 2h and add the new PBS solution of 5ml to keep PBS solution cumulative volume constant, with the content of determined by ultraviolet spectrophotometry basic fibroblast growth factor, release result as shown in Figure 1, show that the release of the wound repair gel bFGF containing hexagonal mesoporous silicon of the present invention comparatively relaxes, and the continuous release time is not less than 24h.
Four, vitro cytotoxicity test (mtt assay):
Arrange blank group (L929 l cell), experimental group (wound repair gel, L929 cell containing hexagonal mesoporous silicon).At 37 DEG C, by material respectively submergence spend the night in the medium, plantation L929 cell is cultivated, and then adds the hatching of MTT/PBS solution; Remove culture medium and MTT with pipet, add DMSO (dimethyl sulfoxide) mixed for several minutes, the solution of orifice plate is detected, record OD value (see Fig. 2).Wound repair gel of the present invention does not produce overt toxicity to L929 l cell as shown in Figure 2.
Claims (7)
1., containing a preparation method for the wound repair gel of hexagonal mesoporous silicon, it is characterized in that, comprise the following steps:
After basic fibroblast growth factor FGF-21, chitosan, hexagonal mesoporous silicon and glyceryl monostearate mix homogeneously, add surfactant and cosurfactant, slow stirring 3 ~ 5min, then dropwise deionized water is dripped, after abundant mixing, with high-shear homogenizer homogenizing 10 ~ 15min under 6000 ~ 8000rpm speed, obtain the emulsion containing mesoporous silicon;
Carbomer is added to the water, leaves standstill after stirring and make that it is fully swelling, be uniformly dispersed formation semi-solid gel; The above-mentioned emulsion containing mesoporous silicon is slowly added wherein, adjust ph to 6.8 ~ 7.5, the obtained wound repair gel containing hexagonal mesoporous silicon.
2. preparation method according to claim 1 prepares a kind of wound repair gel containing hexagonal mesoporous silicon, it is characterized in that, 100g total amount pressed by described wound repair gel, and it is composed as follows: hexagonal mesoporous silicon 0.1 ~ 1g, basic fibroblast growth factor 1 ~ 100 μ g, chitosan 100 ~ 150 μ g, surfactant 15 ~ 20g, cosurfactant 20 ~ 40g, glyceryl monostearate 2 ~ 10g, carbomer 0.5 ~ 5g, all the other are deionized water.
3. a kind of wound repair gel containing hexagonal mesoporous silicon according to claim 2, it is characterized in that, described hexagonal mesoporous silicon obtains by the following method: be dissolved in the beaker of 100ml by 2 ~ 5g lauryl amine, 20 ~ 60ml dehydrated alcohol, 20 ~ 60ml deionized water, use magnetic stirring apparatus rapid stirring; Then add 5 ~ 10g ethyl orthosilicate and stir ageing 30min after 8 ~ 12h; Use deionized water and washing with alcohol respectively, put into high-temperature calcination after oven drying, obtained hexagonal mesoporous Si powder.
4. a kind of wound repair gel containing hexagonal mesoporous silicon according to claim 2, it is characterized in that, described surfactant comprises: more than one in Tween80, sodium lauryl sulphate or polyethyleneglycol glyceride.
5. a kind of wound repair gel containing hexagonal mesoporous silicon according to claim 2, it is characterized in that, described cosurfactant comprises: more than one in ethanol, propylene glycol, isopropyl alcohol or glycerol.
6. a kind of wound repair gel containing hexagonal mesoporous silicon according to claim 2, it is characterized in that, described basic fibroblast growth factor FGF-21 is human alkaline fibroblast growth factor.
7. a kind of wound repair gel containing hexagonal mesoporous silicon according to claim 2, is characterized in that, pH adjusting agent used is one or more in citric acid, malic acid, ethylenediaminetetraacetic acid or triethanolamine.
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| CN106139231A (en) * | 2016-07-29 | 2016-11-23 | 江苏蓝湾生物科技有限公司 | A kind of preparation method of antibacterial mesoporous silicon xerogel material |
| CN106975100A (en) * | 2016-01-15 | 2017-07-25 | 浙江大学 | A kind of nano composite material of cerium oxide/mesoporous silicon and its preparation method and application |
| CN110038156A (en) * | 2019-04-19 | 2019-07-23 | 南通蓝智新材料科技有限公司 | Hemostasis promotees wound healing lotion and preparation method thereof |
| WO2019241985A1 (en) * | 2018-06-22 | 2019-12-26 | 温州医科大学 | Hydrogel, pharmaceutical composition comprising same, and application thereof |
| CN111956506A (en) * | 2019-05-20 | 2020-11-20 | 珀莱雅化妆品股份有限公司 | Active ingredient fixed-point pressing release type skin care pad pasting |
| CN115137867B (en) * | 2022-04-26 | 2023-06-27 | 温州爱恩思生物科技有限公司 | Anti-scar material containing silicon-based inorganic material, preparation method and application |
| CN116350749A (en) * | 2023-06-01 | 2023-06-30 | 内蒙古医科大学附属医院(内蒙古自治区心血管研究所) | A pharmaceutical composition for treating diabetic foot and skin ulcer, and its preparation method |
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| CN111956506A (en) * | 2019-05-20 | 2020-11-20 | 珀莱雅化妆品股份有限公司 | Active ingredient fixed-point pressing release type skin care pad pasting |
| CN115137867B (en) * | 2022-04-26 | 2023-06-27 | 温州爱恩思生物科技有限公司 | Anti-scar material containing silicon-based inorganic material, preparation method and application |
| CN116350749A (en) * | 2023-06-01 | 2023-06-30 | 内蒙古医科大学附属医院(内蒙古自治区心血管研究所) | A pharmaceutical composition for treating diabetic foot and skin ulcer, and its preparation method |
| CN116350749B (en) * | 2023-06-01 | 2023-10-13 | 内蒙古医科大学附属医院(内蒙古自治区心血管研究所) | A pharmaceutical composition for treating diabetic foot and skin ulcer, and its preparation method |
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Effective date of registration: 20211216 Address after: 510663 No. a401, 403 and 405, zone a, Guangzhou International Business Incubator, No. 3, Juquan Road, Science City, Huangpu District, Guangzhou City, Guangdong Province Patentee after: Guangzhou Zhiyuan Biotechnology Co.,Ltd. Address before: 511400 Guangzhou Institute of Industry and Research, Huanshi Dao South Road, Nansha District, Guangzhou City, Guangdong Province Patentee before: SOUTH CHINA University OF TECHNOLOGY |