CN104248658A - Traditional Chinese medicine composition volatile oil and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition volatile oil and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of traditional Chinese medicines and natural medicines, and particularly relates to traditional Chinese medicine composition volatile oil and a preparation method and application thereof. The traditional Chinese medicine composition volatile oil is characterized by comprising the following Chinese herbal medicines by weight: 15-60 parts of mangnolia officinalis and 10-40 parts of perilla leaf. The traditional Chinese medicine composition volatile oil can be prepared into an oral preparation, an inhalant or an externally used ointment by a conventional traditional Chinese medicine preparation method. The beneficial effects of the traditional Chinese medicine composition volatile oil are mainly reflected in that: the traditional Chinese medicine composition volatile oil drug can significantly shorten the forced swimming and tail suspension immobility time of behavioral despair model mice, significantly shorten immobility time in forced swimming test and tail suspension test of chronic stress depression model mice, and significantly increase syrup preference of the chronic stress depression model mice. The result of study shows that the traditional Chinese medicine composition volatile oil has the significant anti experimental depression effect in mice.
Description
Technical field
The present invention relates to pharmaceutical composition, particularly the volatile oil and its preparation method and application of Chinese medicine composition, belongs to the field of Chinese medicines.
Background technology
Depression (depression) is a kind of common mental sickness, has the features such as high prevalence, high relapse rate, high disability rate and high fatality rate.Be embodied in: the long-time depressive emotion continued, and this emotion is significantly more than the limit of necessity, self-doubt, feel the obvious reduction of body energy, cannot realize happy in any interesting activity.This kind of obstacle also can cause the somatic function of patient to lack of proper care, as sleep disordered, local body pain or loss of appetite etc.World Health Organization claims in global range, and total suffer from depression more than 3.5 hundred million people, spreads all over each each age group.Depression is the reason that primarily disables all over the world, and it is the big factors causing global disease to be born.Depression has become the fourth-largest illness in the world, may become be only second to cardiopathic second largest disease to the year two thousand twenty depression.Investigation display in 2012, in state-ownedly suffer from depression in various degree more than 2,600 ten thousand people.At present, the cause of disease that the definite cause of disease of depression causes depression to occur mainly comprises inherited genetic factors, the disorder of neural biochemical metabolism, family and social influence, stress wait.
The medicine of existing depression mainly comprises: tricyclic antidepressant (suppressing the medicine of norepinephrine, serotonin reuptake transporter), as imipramine, amitriptyline, doxepin and Clomipramine etc.; Oxidase inhibitor, as Iproniazid, isocarboxazid, phenelzine and tranylcypromine etc.; Selectivity 5-HT re uptake depressant, as fluoxetine, paroxetine, Sertraline, fluvoxamine and citalopram etc.Existing synthesis antidepressants, usually there will be more untoward reaction during Cure of depression clinically, under therapeutic dose, often make that people is sleepy, xerostomia, blurred vision, constipation, palpitating speed, dysuria and postural hypotension; Accidental serious cardiovascular side effects, urine retention and enteroparalysis; It is excessive that to cause acute poisoning even dead.Chinese medicine have accumulated abundant clinical experience in control depression, but antidepressant Chinese patent medicine is in the market few, still lacks the Chinese patent medicine of the little Cure of depression of untoward reaction evident in efficacy.
Summary of the invention
Goal of the invention
Volatile oil that the invention provides Chinese medicine composition and preparation method thereof, the volatile oil of this Chinese medicine composition extracts preparation by Cortex Magnoliae Officinalis and Folium Perillae, and the application of this medicine in preparation medicament for treatment of depression.
Technical scheme
Chinese medicine composition of the present invention is made up of 2 taste Chinese medicines, is respectively Cortex Magnoliae Officinalis and Folium Perillae.
The volatile oil of Chinese medicine composition provided by the invention, be made up of the crude drug of following weight portion:
Cortex Magnoliae Officinalis 15-60 part, Folium Perillae 10-40 part.
The volatile oil of Chinese medicine composition provided by the invention, is characterized in that:
Obtain as follows:
(1) each raw material is taken by following weight parts: Cortex Magnoliae Officinalis 15-60 part, Folium Perillae 10-40 part;
(2) 6 hours are soaked by 6-10 times of weight water after medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil.
The volatile oil of Chinese medicine composition of the present invention can add various adjuvants required when preparing different dosage form, such as disintegrating agent, lubricant, adhesive, aerosol, emulsifying agent etc., be prepared into any one conventional oral formulations, inhalant or externally used paste with the method for Chinese medicinal of routine.Described oral formulations is drop pill, Emulsion, tablet, capsule, soft capsule, granule, pill or oral liquid, and described inhalant is aerosol, and described external preparation is rubber-emplastrum.
Preferably, a kind of method preparing medicine provided by the invention is as follows:
(1) each raw material is taken by following weight parts: Cortex Magnoliae Officinalis 30 parts, Folium Perillae 20 parts;
(2) 6 hours are soaked by 8 times of weight water after medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil, for subsequent use;
(3) Polyethylene Glycol substrate is appropriate, is heated to melt, then adds the volatile oil of above-mentioned Chinese medicine composition, and mixing, makes drop pill, to obtain final product.
The usage of medicine of the present invention and consumption: the dosage of medicine of the present invention depends on concrete dosage form, and the factor such as the age of patient, body weight, health status.As guidance: drop pill, oral, one time 1 bag (7.5g crude drug/bag), 2 times on the one 6 weeks is a course for the treatment of.
Beneficial effect
1, the antidepressant beneficial effect of the volatile oil of Chinese medicine composition of the present invention is mainly reflected in: result of study display of the present invention, and medicine of the present invention significantly shortens desperate model mice forced swimming and outstanding tail dead time; The remarkable shortening chronic stress depression model dead time of mice in forced swim test and tail-suspention test, significantly increase the sucrose solution preference of chronic stress depression model mice.Medicine of the present invention has the effect of significant anti-mouse depression model.
Detailed description of the invention
Further describe the present invention by the following examples, it should be understood that these embodiments only for the object of illustration, never limit the scope of the invention.
The preparation of embodiment 1 drop pill
Each raw material is taken: Cortex Magnoliae Officinalis 300g, Folium Perillae 200g by following weight;
6 hours are soaked by 8 times of weight water by after said medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil.Taking polyethylene glycol substrate is appropriate, is heated to melt, then adds the volatile oil of Chinese medicine composition, and mixing, makes drop pill, to obtain final product.
The preparation of embodiment 2 Emulsion
Each raw material is taken: Cortex Magnoliae Officinalis 300g, Folium Perillae 200g by following weight;
6 hours are soaked by 8 times of weight water by after said medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil.Get 0.3% sodium benzoate and sweeting agent, arabic gum is appropriate, under strong stirring, add Chinese medicine composition volatile oil and Tween-80 are appropriate, and add water to 1000ml, continue stirring 10 minutes, subpackage, to obtain final product.
The preparation of embodiment 3 aerosol
Each raw material is taken: Cortex Magnoliae Officinalis 300g, Folium Perillae 200g by following weight;
6 hours are soaked by 8 times of weight water by after said medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil.The volatile oil of Chinese medicine composition is settled to 1000ml with dehydrated alcohol, add 15% non-ionic surface active agent ethanol solution 300ml again, after miscible under constant agitation, quantitative separating, in aerosol pressure vessel, is pressed into the propellant F through filtering with microporous membrane under 800 ~ 1000kPa pressure in bottle after gland
12, to obtain final product.
The preparation of embodiment 4 rubber-emplastrum
Each raw material is taken: Cortex Magnoliae Officinalis 300g, Folium Perillae 200g by following weight;
6 hours are soaked by 8 times of weight water by after said medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil.The volatile oil of Chinese medicine composition is added the substrate be made up of rubber, Colophonium, zinc oxide, vaseline and lanoline that about 4 times amount are heavy, make coating, carry out painting cream, cutting, lid lining, is cut into small pieces, obtains final product.
The volatile oil of embodiment 5 Chinese medicine composition of the present invention is on the impact test of the desperate behavior of mice
1 experiment material
1.1 laboratory animal
Cleaning grade male ICR mouse, body weight (20 ± 2g).Laboratory animal is provided by Yangzhou University's animal reproduction center, laboratory animal production licence: SCXK (Soviet Union) 2007.0001.The free diet of whole experimentation animal and drinking-water.Room temperature 25 DEG C, environment quiet.
1.2 Experimental agents
By reagent: the volatile oil of Chinese medicine composition of the present invention.
Fluoxetine Hydrochloride: Pharmaceutical Co Ltd, Changzhou Pharmaceutical Factory No.4 produces, lot number: 20120308.
Suspension will be ground to form by reagent and fluoxetine 0.5%CMC-Na before administration, and be placed in 4 DEG C of refrigerators and save backup.
1.3 instrument
Stopwatch, 2500mL beaker, outstanding tail support, electronic balance (Beijing Sai Duolisi balance company limited), gastric perfusion needle, Open field activity case (diameter 30cm, high 20cm).
2 experimental techniques
The extraction of the volatile oil of 2.1 Chinese medicine compositions
Get Cortex Magnoliae Officinalis 300g, Folium Perillae 200g, 6 hours are soaked by 8 times of weight water by after said medicine mixing, then flask and bead number is placed in, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil 1.6ml.
2.2 grouping and administrations
Mice is divided into 4 groups at random, often organize 10, be respectively blank group (0.5%CMC-Na solution), positive drug group (fluoxetine: 10mg/kg), the volatile oil low dose group (crude drug amount 1.25g/kg) of Chinese medicine composition, volatile oil high dose group (crude drug amount 2.5g/kg), administration volume is 10ml/kg body weight, and continuous gastric infusion detected after 7 days.
2.3 tail-suspention test
Each group of continuous gastric infusion of mice 7 days, after last administration 1 hour, is inverted mice, the position of mice tail end 2cm is pasted just on a horizontal waddy, make mice become reversal of the natural order of things state, its head distance table top is about 15cm, by the sight line between dividing plate space between adjacent animal.Observe 6min, the accumulation dead time of the interior each group mice of 4min after record.Dead time criterion: mice is draped period, without location dead time during any activity.
2.4 forced swim test
Each group of continuous gastric infusion of mice 7 days, after last administration, is placed in the glass cylinder of depth of water 10cm, water temperature 25 DEG C by mice, animal sight line is separated with baffle plate between every two mices, to prevent mutual interference, when just putting into beaker, mice swimming is struggled, after a period of time, because of despair, activity weakens, and showing as the micro-song of health, surfaces in nostril, in floating state, extremity are motionless.Observe 6min, respectively organize the accumulation dead time of mice in rear 4min.Dead time judges: mice swims in the water surface, does not struggle, and only does some and head must be kept in the action of the water surface.
2.5 Open field activity
Each group of continuous gastric infusion of mice 7 days, after last administration, by mice as the open case of mice.Experimental provision is diameter 30cm, the cylinder of high 20cm, this laboratory observation is carried out under quiet environment, observe 4min, respectively to organize after mice horizontal score (wearing lattice number of times), vertical score (the two forelimb of mice upwards praises number of times) in 2min, modify number of times (mice, in clean health each ministries and commissions process, uses the various motion that head, tongue and forelimb are made).
2.6 statistical procedures
All data all represent with mean ± standard deviation (Mean ± SD), one-way analysis of variance (ANOVA) is adopted to carry out statistical analysis to experimental data, P < 0.05 is for having significant difference, and P < 0.01 is for having pole significant difference.
3 experimental results
The volatile oil of 3.1 Chinese medicine compositions is on the impact of desperate model mice tail-suspention test dead time
As shown shown in 1-1, continuous gastric infusion 7 days, the volatile oil (crude drug amount 2.5g/kg) of Chinese medicine composition significantly can shorten the dead time of tail-suspention test small mouse; Positive control drug fluoxetine (10mg/kg) significantly can shorten the dead time of mice equally in tail-suspention test.
Table 1-1 Chinese medicine composition volatile oil is on the impact (Mean ± SD, n=10) of desperate model mice tail-suspention test dead time
Note: compared with blank group, * P < 0.05, * * P < 0.01
The volatile oil of 3.2 Chinese medicine compositions is on the impact of desperate model mice forced swim test dead time
As shown shown in 1-2, continuous gastric infusion 7 days, the volatile oil (crude drug amount 2.5g/kg) of Chinese medicine composition significantly can shorten the dead time of mice in forced swim test; Positive control drug fluoxetine (10mg/kg) can shorten the dead time of mice equally in forced swim test.
Table 1-2 Chinese medicine composition volatile oil is on the impact (Mean ± SD, n=10) of desperate model mice forced swim test dead time
Note: compared with blank group, * P < 0.05, * * P < 0.01
The volatile oil of 3.3 Chinese medicine compositions is on the impact of spontaneous activity in mice
Experimental result is in Table 1-3, volatile oil (the crude drug amount 1.25g/kg of Chinese medicine composition, 2.5g/kg) and fluoxetine (10mg/kg) continuously gastric infusion 7 days, in Open field activity, each group of spontaneous activity in mice compares with matched group does not have significant change, show that the volatile oil of Chinese medicine composition has no significant effect spontaneous activity in mice, its antidepressant effect has specificity.
The thin compound volatile oil of table 1-3 Chinese medicine is on the impact (Mean ± SD, n=10) of mice score in Open field activity
Note: compared with blank group, * P < 0.05, * * P < 0.01
The volatile oil of embodiment 6 Chinese medicine composition of the present invention is tested the impact of chronic stress depression model mice
1 experiment material
1.1 laboratory animal
Cleaning grade male ICR mouse, body weight (20 ± 2g).Laboratory animal is provided by Yangzhou University's animal reproduction center, laboratory animal production licence: SCXK (Soviet Union) 2007.0001.The free diet of whole experimentation animal and drinking-water.Room temperature 25 DEG C, environment quiet.
1.2 Experimental agents
By reagent: the volatile oil of Chinese medicine composition of the present invention.
Fluoxetine Hydrochloride: Pharmaceutical Co Ltd, Changzhou Pharmaceutical Factory No.4 produces, lot number: 20120308.
Suspension will be ground to form by reagent and fluoxetine 0.5%CMC-Na before administration, and be placed in 4 DEG C of refrigerators and save backup.
1.3 instrument
Stopwatch, 2500mL beaker, outstanding tail support, electronic balance (Beijing Sai Duolisi balance company limited), gastric perfusion needle, Open field activity case (diameter 30cm, high 20cm).
2 experimental techniques
The foundation of the unpredictable Stress model of 2.1 chronic gentleness
Chronic gentleness stimulates and comprises: (1) of short duration same cage is raised (every cage 5 or 6) 3h, (2) mouse cage (10min) is rocked, (3) noise (radio of non-frequency modulation) 3h, (4) rearging cage tilts (about 30 °) 8h or spend the night, (5) moist bedding and padding (150ml water) 8h or spend the night, (6) foreign body 8h or spend the night, (7) illumination is spent the night, (8) tail (5min) is pressed from both sides, (9) constraint (10min).
Volatile oil each dosage group mice of model group, positive drug group and Chinese medicine composition continues the above-mentioned chronic gentleness of application stress 6 weeks, application every day above 2 different stress.Stress kind and order random selecting.Naive mice does not carry out chronic gentle stress stimulation.
2.2 grouping and administrations
Mice carries out Open field activity screening on pretreatment, selects the mice that neurological deficit score in Open field activity is close, is divided into 5 groups at random, often organizes 12.Model group accepts 42 days stress stimulations altogether, and latter 21 day every day, gavage gave 0.5%CMC-Na (10ml/kg); Chinese medicine composition volatilization line of oils accepts 42 days stress stimulations, gastric infusion latter 21 day every day (crude drug amount 1.25g/kg, 2.5g/kg) altogether; Positive drug group accepts 42 days stress stimulations altogether, and latter 21 day every day, gavage gave fluoxetine (10mg/kg); Blank group does not accept any stress stimulation and raises 42 days, and latter 21 day every day, gavage gave 0.5%CMC-Na (10ml/kg).
2.3 Open field activity
After chronic stress experiment terminates, detect the change of each group of mice behavior with Open field activity.By mice as the open case of mice, observe 4min, respectively to organize after mice horizontal score (wearing lattice number of times), vertical score (the two forelimb of mice upwards praises number of times) in 2min, modify number of times (mice, in clean health each ministries and commissions process, uses the various motion that head, tongue and forelimb are made).Behavior rating adopts mono blind method, and observation chronic stress and medicine are on the impact of spontaneous activity in mice ability.
2.4 forced swim test
After chronic stress terminates, each group mice carries out forced swim test.Mice is placed in the glass cylinder of depth of water 10cm, water temperature 25 DEG C, observes 6min, respectively organize the accumulation dead time of mice in rear 4min.Dead time judges: mice swims in the water surface, does not struggle, and only does some and head must be kept in the action of the water surface.
2.5 tail-suspention test
After chronic stress terminates, each group mice carries out tail-suspention test.Pasted just on a horizontal waddy at the position of mice tail end 2cm, make mice become reversal of the natural order of things state, its head distance table top is about 15cm, by the sight line between dividing plate space between adjacent animal.Observe 6min, the accumulation dead time of the interior each group mice of 4min after record.Dead time criterion: mice is draped period, without location dead time during any activity.
2.6 sucrose solution preference experiments
After chronic stress terminates, each group mice carries out the mensuration of the sucrose solution consumption of each group of mice.Start to measure front taboo water, fasting 24h, then give the aqueous sucrose solution of 1% (w/v), aqueous sucrose solution is placed in 2 identical drinking bottles respectively, carries out the mensuration of sucrose solution preference after 24h.During measurement, the single cage of mice is raised, and 1, every cage, is equipped with common drinking water and 1% aqueous sucrose solution respectively in 2 drinking bottles, weighs the consumption of mice common drinking water, 1% sucrose water and total liquid after 24h.Relatively normal mouse, chronic stress mice and give the difference of mice in sucrose solution consumption and sucrose solution preference of Chinese medicine composition volatile oil and fluoxetine.
Sucrose solution preference (Sucrose Preference, SP) represents with consumption (g) × 100% of the consumption (g) of aqueous sucrose solution/total drinking water.
2.7 statistical procedures
All data all represent with mean ± standard deviation (Mean ± SD), one-way analysis of variance (ANOVA) is adopted to carry out statistical analysis to experimental data, P < 0.05 is for having significant difference, and P < 0.01 is for having pole significant difference.
3 experimental results
3.1 Open field activity
As shown shown in 2-1, after the chronic gentleness entered 42 days stimulates, compared with blank group mice, the spontaneous activity of model group mice obviously reduces, and shows as and wears lattice number of times, uprightly number of times, modification number of times obviously reduces; Give Chinese medicine composition volatile oil (crude drug amount 1.25g/kg) after 21 days, compared with model group mice, upright number of times and the modification number of times of mice all have remarkable increase; Give Chinese medicine composition volatile oil (crude drug amount 2.5g/kg) after 21 days, compared with model group mice, wearing lattice number of times, upright number of times and modifying number of times of mice all has remarkable increase; Positive drug group (fluoxetine, 10mg/kg) also significantly can increase the spontaneous activity of mice.
Table 2-1 Chinese medicine composition volatile oil is on the impact (Mean ± SD, n=12) of chronic stress spontaneous activity in mice
Note: compared with model group, * P < 0.05, * * P < 0.01; Compared with blank group,
#p < 0.05,
##p < 0.01
3.2 forced swim test
As shown shown in 2-2, compared with blank group, after the chronic stress of 42 days, model group mice dead time significant prolongation in forced swim test, prompting chronic stress can increase the behavioral despair degree of mice; Within continuous 3 weeks, give Chinese medicine composition volatile oil (crude drug amount 1.25g/kg, 2.5g/kg) and significantly can shorten the dead time of depressed mice in forced swim test, show that Chinese medicine composition volatile oil significantly can improve the desperate behavior of chronic stress mice; Positive controls (fluoxetine, 10mg/kg) significantly can improve the desperate behavior of depressed mice equally.
Table 2-2 Chinese medicine composition volatile oil is on the impact (Mean ± SD, n=12) of chronic stress mouse forced swimming test dead time
Note: compared with model group, * P < 0.05, * * P < 0.01; Compared with blank group,
#p < 0.05,
##p < 0.01
3.3 tail-suspention test
As shown shown in 2-3, compared with blank group, after the chronic stress of 42 days, the dead time significant prolongation of mice in tail-suspention test (p < 0.01), shows that chronic stress can increase the weight of the behavioral despair degree of mice; Give Chinese medicine composition volatile oil (crude drug amount 1.25g/kg continuously, 2.5g/kg) 21 days significantly can shorten the dead time of mice in tail-suspention test, and display Chinese medicine composition volatile oil significantly can improve the desperate behavior of chronic stress depression model mice; Give positive drug (fluoxetine, 10mg/kg) continuously and there is the effect improving the desperate behavior of depressed mice for 21 days equally.
Table 2-3 Chinese medicine composition volatile oil is on the impact (Mean ± SD, n=12) of chronic stress Tail suspension test dead time
Note: compared with model group, * P < 0.05, * * P < 0.01; Compared with blank group,
#p < 0.05,
##p < 0.01
3.4 sucrose solution preference experiments
Suffer the mice of chronic stress to occur the phenomenon that sucrose diseases caused by retention of fluid consumption declines, this phenomenon is referred to as anhedonia.Depressed, the hebetude that show with patients with depression have certain similarity.Sucrose solution preference is the ratio of mice sucrose diseases caused by retention of fluid consumption and whole drinking water amount of drinking in 24h, and this value has reacted the degree of mice to the prioritizing selection of sucrose solution.As shown in Table 2-4, the chronic stress of 42 days causes the sucrose solution preference of mice significantly to reduce (P < 0.01), compared with model group, within continuous 21 days, give the sucrose solution preference that Chinese medicine composition volatile oil (crude drug amount 1.25g/kg, 2.5g/kg) significantly can increase the depressed mice of chronic stress; The sucrose solution preference of positive controls (fluoxetine, 10mg/kg) mice also has remarkable increase compared with model group.Display Chinese medicine composition volatile oil can improve the emotion of chronic depression mice, the mice hebetude that antagonism depressive state causes and anhedonia.
Table 2-4 Chinese medicine composition volatile oil is on the impact (Mean ± SD, n=12) of chronic stress mice sucrose solution preference
Note: compared with model group, * P < 0.05, * * P < 0.01; Compared with blank group,
#p < 0.05,
##p < 0.01.
Claims (5)
1. the volatile oil of Chinese medicine composition, is characterized in that:
Obtain as follows:
(1) each raw material is taken by following weight parts: Cortex Magnoliae Officinalis 15-60 part, Folium Perillae 10-40 part;
(2) 6 hours are soaked by 6-10 times of weight water after medicine mixing, then be placed in flask and bead number, after jolting mixing, connect volatile oil determination apparatus and reflux condensing tube, autocondensation pipe upper end adds water and makes to be full of the scale part of volatile oil determination apparatus, and when overflow enters flask till.Put in electric jacket and be slowly heated to boil, and keep micro-and boil 5 hours, no longer increase to oil mass, stop heating, be separated after sufficient standing, collect the oily liquids of yellow, as clear as crystal, taste giving off a strong fragrance, with anhydrous sodium sulfate dehydration, obtained volatile oil.
2. according to the volatile oil of the Chinese medicine composition of claim 1, it is characterized in that, the weight portion of each crude drug is: Cortex Magnoliae Officinalis 30 parts, Folium Perillae 20 parts.
3., according to the volatile oil of the Chinese medicine composition of claim 1 or 2, oral formulations, inhalant or externally used paste can be prepared into according to Chinese medicine conventional formulation method.
4. according to claim 3, it is characterized in that, described oral formulations is drop pill, Emulsion, tablet, capsule, soft capsule, granule, pill or oral liquid; Described inhalant is aerosol, and described external preparation is rubber-emplastrum.
5. the application of volatile oil in preparation medicament for treatment of depression of any one Chinese medicine composition of claim 1-4.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104987954A (en) * | 2015-05-31 | 2015-10-21 | 陕西理工学院 | Method for extracting volatile oil and magnolol from mangnolia officinalis leaves |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104987954A (en) * | 2015-05-31 | 2015-10-21 | 陕西理工学院 | Method for extracting volatile oil and magnolol from mangnolia officinalis leaves |
| CN104987954B (en) * | 2015-05-31 | 2021-02-09 | 陕西理工大学 | Method for extracting volatile oil and magnolol from magnolia officinalis leaves |
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