CN104225645A - Microbial preparation for purifying animal breeding environment and preparation method thereof - Google Patents
Microbial preparation for purifying animal breeding environment and preparation method thereof Download PDFInfo
- Publication number
- CN104225645A CN104225645A CN201410415629.2A CN201410415629A CN104225645A CN 104225645 A CN104225645 A CN 104225645A CN 201410415629 A CN201410415629 A CN 201410415629A CN 104225645 A CN104225645 A CN 104225645A
- Authority
- CN
- China
- Prior art keywords
- rhodospirillum
- cultivation environment
- microorganism formulation
- preparation
- purifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention provides a microbial preparation for purifying the animal breeding environment. The microbial preparation is prepared from a compound microorganism bacterium agent, a hydroxycarboxylic acid chelating agent, polyol non-ionic hydrophilic surfactant in a mass ratio of (60-90):(9-25):(1-15), wherein the compound microorganism bacterium agent is a mixed bacterium agent which is prepared from bacteria of bacillus and bacteria of rhodospirillum, and the bacillus bacterium agent and the rhodospirillum bacterium agent are in a mass ratio of 2.5-7. By adopting the compound microorganism bacterium agent, growth of putrefying bacteria is inhibited, the decomposing means of organic matters is improved, and the burst sizes of ammonia and hydrogen sulfide and generation of amine substances can be reduced; and by adopting the hydrophilic surfactant and the hydroxycarboxylic acid chelating agent, stink molecules can be dissolved and absorbed, so that the odor in the environment can be reduced, and propagation of mosquitoes and flies can be reduced.
Description
Technical field
The invention belongs to field of agricultural environmental protection, particularly relating to a kind of microorganism formulation for purifying animal cultivation environment and preparation method thereof.
Background technology
Along with the development of China's scale, intensive animal husbandry, the pollution of the foul smell in Production of Livestock and Poultry to environment constantly aggravates.Not only cause great threat to the health of the mankind, also drastically influence the health of poultry and the performance of fertility performance, animal farm odor pollution more and more causes the attention of people.
In the past, administer the way of foul smell and mostly adopt and manually buries, or with chemicals and perfume sprays smelly with perfume (or spice) screening, can not thoroughly deodorization, secondary pollution can be caused because of residuals again.At present, a focus of research has been become for animal farm foul smell research and development deodorizer.The deodorizer worked out both at home and abroad has reached more than 20 and has planted, and has plenty of directly to join in fowl poultry kind urine and carries out deodorization, as zeolite, Melanteritum, calcium phosphate pH buffer agent, potassium permanganate, hydrogen peroxide etc.; Has plenty of the object of adding to by providing the digestibility of diet nutrient material to reach deodorization in poultry diet, as Yucca position extracting solution (deodorase), enzyme preparation, zeolite, active carbon, acid supplement etc.
Deodorizer mainly contains following several types: absorbent-type deodorizer, perfume masking type deodorizer, chemical deodorizing agent, enzyme preparation deodorizer, microbiocidal deodorizer, galenical deodorizer, composite type deodorization agent etc., and wherein the deodorization principle of microbial deodorant is, with microorganism, the stink substance forming malodor source is decomposed the object just fundamentally reaching deodorization like this.Such as patent of invention contains deodorizer as the dry cell of microorganism of active component and deodorizing method, old pool, the artificial sea of invention is true, patent publication No. is CN1636603A, the antibacterial of the preferred Escherichia of this patent, brevibacterium and bacillus, makes dry cell of microorganism deodorizer by fermentation, heat sterilization.
Summary of the invention
The present invention solves the aforementioned problems in the prior proposition, its objective is and provides a kind of microorganism formulation for purifying animal cultivation environment.
Another object of the present invention is to provide a kind of manufacture method of the microorganism formulation for purifying animal cultivation environment.
For achieving the above object, the present invention is by the following technical solutions:
For purifying a microorganism formulation for animal cultivation environment, it is characterized in that, it comprises: complex micro organism fungicide, hydroxy carboxylic acid quasi-chelate compound, polyol type non-ionic hydrophilic surfactant; Described complex micro organism fungicide is be selected from the antibacterial of bacillus and be selected from the mix bacterium agent of antibacterial after culture medium fermentation of Rhodospirillum; The mass ratio of described complex micro organism fungicide, described hydroxy carboxylic acid quasi-chelate compound, described polyol type non-ionic hydrophilic surfactant is: 60-90:9-25:1-15, and wherein the mass ratio of rhodospirillum microbial inoculum and gemma bacillus agent is 2.5-7:1.
Preferably, the antibacterial of described bacillus is Bafillus natt.
Preferably, the antibacterial of described Rhodospirillum is Rhodopseudomonas palustris.
Preferably, described hydroxy carboxylic acid quasi-chelate compound is one or more of citric acid, tartaric acid and gluconic acid.
Preferably, described polyol type non-ionic hydrophilic surfactant be spans span-20,40,60,80 and/or Tweens Tween-20,40,60, in 80 nonionic surfactant any one or multiple.
The manufacture method of a kind of microorganism formulation for purifying animal cultivation environment as described above, it is characterized in that, the method comprises the steps:
(1) preparation of gemma bacillus agent: the preparation of raw material by following percentage by weight: the antibacterial 1-3wt% of bacillus, culture medium 97-99wt%, carries out 37 DEG C of aerobic fermentation 1-3 days, preferably 2 days;
(2) preparation of rhodospirillum microbial inoculum: the preparation of raw material by following percentage by weight: the antibacterial 10-20wt% of Rhodospirillum, culture medium 80-90wt%, carries out 32 DEG C of anaerobism illumination fermentations 8-12 days, preferably 10 days;
(3) gemma bacillus agent, rhodospirillum microbial inoculum, hydroxy carboxylic acid quasi-chelate compound, polyol type non-ionic hydrophilic surfactant are mixed by following percentage by weight: gemma bacillus agent 10-20wt%, rhodospirillum microbial inoculum 50-70wt%, hydroxy carboxylic acid quasi-chelate compound 9-25wt%, polyol type non-ionic hydrophilic surfactant 1-15wt%, obtain the microorganism formulation for purifying animal cultivation environment.
Preferably, the antibacterial of described bacillus is Bafillus natt.
Preferably, the antibacterial of described Rhodospirillum is Rhodopseudomonas palustris.
Preferably, described hydroxy carboxylic acid quasi-chelate compound is one or more of citric acid, tartaric acid and gluconic acid.
Preferably, described polyol type non-ionic hydrophilic surfactant be spans span-20,40,60,80 and/or Tweens Tween-20,40,60, any one or a few in 80 nonionic surfactant.
Preferably, culture medium described in (1) step is at least two kinds of mixture of bean cake powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, ammonium sulfate, carbamide, sterilized water, and its percentage by weight is: bean cake powder 2-4wt%, peptone 0.5-1wt%, Semen Maydis powder 3-5wt%, glucose 0-5wt%, sodium hydrogen phosphate 0.2-0.5wt%, potassium dihydrogen phosphate 0.1-0.5wt%, ammonium sulfate 0.1-0.5wt%, carbamide 0.1-0.5wt%, sterilized water 83-94wt%.
Preferably, culture medium described in (2) step is at least two kinds of mixture of molasses, sodium bicarbonate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its percentage by weight is: molasses 5-10wt%, sodium bicarbonate 5-10wt%, potassium dihydrogen phosphate 0.1-0.5wt%, magnesium sulfate 0.05-0.1wt%, sterilized water 79.4-96wt%.
The preferred Bafillus natt of the present invention (Bacillus natto) is the preservation of Chinese agriculture Culture Collection, and bacterial strain number is ACCC10152.
The preferred Rhodopseudomonas palustris of the present invention (Rhodopseudomonas palustris) is the preservation of Chinese agriculture Microbiological Culture Collection administrative center, and bacterial strain number is ACCC10649; Rhodopseudomonas palustris thalline is nutritious, protein content is up to 65%, be rich in various bioactivators, have the suitability strong, can the organic wastewater of enduring high-concentration and higher decomposition and inversion ability, the poisonous substance such as phenol, cyanogen is also had to the features such as certain tolerance Sum decomposition ability.
A kind of microorganism formulation for purifying animal cultivation environment of the present invention, the ultimate principle of process foul smell utilizes hydrophilic surfactant active to be dissolved in more fully in water by foul gas, foul smell soluble in water is absorbed by hydroxy carboxylic acid quasi-chelate compound, and water-fast foul smell is decomposed into solable matter, absorbs, thoroughly foul smell molecule is eliminated.
The hydroxy carboxylic acid quasi-chelate compound optimization citric acid that the present invention adopts, be commercially available citric acid monohydrate, require food stage, content is 90wt% at least.The preferred tween 80 of the present invention also requires as food stage.
The present invention adopts technique scheme, compared with prior art, has following technique effect:
1, the present invention adopts complex micro organism fungicide, inhibits the growth of Putrefying bacteria, improves organic decomposition approach, reduces ammonia and the burst size of hydrogen sulfide and the generation of amine substance;
2, adopt hydrophilic surfactant active and hydroxy carboxylic acid quasi-chelate compound dissolve and sponge foul smell molecule, thus alleviate the stench in environment, reduce mosquitos and flies twin.
Detailed description of the invention
The invention provides a kind of complex microorganism diseases prevention insect-proof agent for green vegetable and preparation method thereof.
Carry out detailed and concrete introduction below by specific embodiment to the present invention, to make better to understand the present invention, but following embodiment does not limit the scope of the invention.
Embodiment 1
The present embodiment provides a kind of microorganism formulation for purifying animal cultivation environment, follows these steps to make:
(1) preparation of Bafillus natt microbial inoculum: the preparation of raw material by following percentage by weight: Bafillus natt kind 3wt%, culture medium 97wt%, carries out 37 DEG C of aerobic fermentations 2 days, and after fermentation, liquid is stand-by;
Culture medium described in (1) step is at least two kinds of mixture of bean cake powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, ammonium sulfate, carbamide, sterilized water, and its percentage by weight is: bean cake powder 2wt%, peptone 0.5wt%, Semen Maydis powder 3wt%, glucose 1wt%, sodium hydrogen phosphate 0.2wt%, potassium dihydrogen phosphate 0.1wt%, ammonium sulfate 0.2wt%, carbamide 0.1wt%, sterilized water 92.9wt%.
(2) preparation of Rhodopseudomonas palustris microbial inoculum: the preparation of raw material by following percentage by weight: Rhodopseudomonas palustris 20wt%, culture medium 80wt%, carries out 32 DEG C of anaerobism illumination and ferments 10 days;
Culture medium described in (2) step is at least two kinds of mixture of molasses, sodium bicarbonate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its percentage by weight is: molasses 5wt%, sodium bicarbonate 5wt%, potassium dihydrogen phosphate 0.1wt%, magnesium sulfate 0.02wt%, sterilized water 89.88wt%.
(3) Bafillus natt microbial inoculum, Rhodopseudomonas palustris microbial inoculum, citric acid, tween 80 are mixed by following percentage by weight: Bafillus natt microbial inoculum 10wt%, Rhodopseudomonas palustris microbial inoculum 70wt%, citric acid 15wt%, tween 80 5wt%, obtain the microorganism formulation for purifying animal cultivation environment.
Embodiment 2
The present embodiment provides a kind of microorganism formulation for purifying animal cultivation environment, follows these steps to make:
(1) preparation of Bafillus natt microbial inoculum: the preparation of raw material by following percentage by weight: Bafillus natt kind 1wt%, culture medium 99wt%, carries out 37 DEG C of aerobic fermentations 2 days, and after fermentation, liquid is stand-by;
Culture medium described in (1) step is at least two kinds of mixture of bean cake powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, ammonium sulfate, carbamide, sterilized water, and its percentage by weight is: bean cake powder 4wt%, peptone 1wt%, Semen Maydis powder 5wt%, glucose 1wt%, sodium hydrogen phosphate 0.5wt%, potassium dihydrogen phosphate 0.2wt%, ammonium sulfate 0.2wt%, carbamide 0.1wt%, sterilized water 88wt%.
(2) preparation of Rhodopseudomonas palustris microbial inoculum: the preparation of raw material by following percentage by weight: Rhodopseudomonas palustris 15wt%, culture medium 85wt%, carries out 32 DEG C of anaerobism illumination and ferments 10 days;
Culture medium described in (2) step is at least two kinds of mixture of molasses, sodium bicarbonate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its percentage by weight is: molasses 10wt%, sodium bicarbonate 10wt%, potassium dihydrogen phosphate 0.5wt%, magnesium sulfate 0.1wt%, sterilized water 79.4wt%.
(3) Bafillus natt microbial inoculum, Rhodopseudomonas palustris microbial inoculum, citric acid, tween 80 are mixed by following percentage by weight: Bafillus natt microbial inoculum 20wt%, Rhodopseudomonas palustris microbial inoculum 60wt%, citric acid 15wt%, tween 80 5wt%, obtain the microorganism formulation for purifying animal cultivation environment.
(if having, the comparative example producing above-mentioned technique effect please be provide)
Be described in detail specific embodiments of the invention above, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (8)
1. for purifying a microorganism formulation for animal cultivation environment, it is characterized in that, it comprises: complex micro organism fungicide, hydroxy carboxylic acid quasi-chelate compound, polyol type non-ionic hydrophilic surfactant; Described complex micro organism fungicide is be selected from the antibacterial of bacillus and be selected from the mix bacterium agent of antibacterial after culture medium fermentation of Rhodospirillum; The mass ratio of described complex micro organism fungicide, described hydroxy carboxylic acid quasi-chelate compound, described polyol type non-ionic hydrophilic surfactant is: 60-90:9-25:1-15, and wherein the mass ratio of rhodospirillum microbial inoculum and gemma bacillus agent is 2.5-7:1.
2. the microorganism formulation for purifying animal cultivation environment according to claim 1, is characterized in that, the antibacterial of described bacillus is Bafillus natt.
3. the microorganism formulation for purifying animal cultivation environment according to claim 1, is characterized in that, the antibacterial of described Rhodospirillum is Rhodopseudomonas palustris.
4. the microorganism formulation for purifying animal cultivation environment according to claim 1, is characterized in that, described hydroxy carboxylic acid quasi-chelate compound is one or more of citric acid, tartaric acid and gluconic acid.
5. the microorganism formulation for purifying animal cultivation environment according to claim 1, it is characterized in that, described polyol type non-ionic hydrophilic surfactant be spans span-20,40,60,80 and/or Tweens Tween-20,40,60, any one or a few in 80 nonionic surfactant.
6. the manufacture method of the microorganism formulation for purifying animal cultivation environment as described in claim 1-5, it is characterized in that, the method comprises the steps:
(1) preparation of gemma bacillus agent: the preparation of raw material by following percentage by weight: the antibacterial 1-3wt% of bacillus, culture medium 97-99wt%, carries out 37 DEG C of aerobic fermentation 1-3 days;
(2) preparation of rhodospirillum microbial inoculum: the preparation of raw material by following percentage by weight: the antibacterial 10-20wt% of Rhodospirillum, culture medium 80-90wt%, carries out 32 DEG C of anaerobism illumination fermentation 8-12 days;
(3) gemma bacillus agent, rhodospirillum microbial inoculum, hydroxy carboxylic acid quasi-chelate compound, polyol type non-ionic hydrophilic surfactant are mixed by following percentage by weight: gemma bacillus agent 10-20wt%, rhodospirillum microbial inoculum 50-70wt%, hydroxy carboxylic acid quasi-chelate compound 9-25wt%, polyol type non-ionic hydrophilic surfactant 1-15wt%, obtain the microorganism formulation for purifying animal cultivation environment.
7. the manufacture method of the microorganism formulation for purifying animal cultivation environment according to claim 6, it is characterized in that, culture medium described in (1) step is bean cake powder, peptone, Semen Maydis powder, glucose, potassium dihydrogen phosphate, sodium hydrogen phosphate, ammonium sulfate, carbamide, at least two kinds of mixture of sterilized water, its percentage by weight is: bean cake powder 2-4wt%, peptone 0.5-1wt%, Semen Maydis powder 3-5wt%, glucose 0-5wt%, sodium hydrogen phosphate 0.2-0.5wt%, potassium dihydrogen phosphate 0.1-0.5wt%, ammonium sulfate 0.1-0.5wt%, carbamide 0.1-0.5wt%, sterilized water 83-94wt%.
8. the manufacture method of the microorganism formulation for purifying animal cultivation environment according to claim 6, it is characterized in that, culture medium described in (2) step is at least two kinds of mixture of molasses, sodium bicarbonate, ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, sterilized water, and its percentage by weight is: molasses 5-10wt%, sodium bicarbonate 5-10wt%, potassium dihydrogen phosphate 0.1-0.5wt%, magnesium sulfate 0.05-0.1wt%, sterilized water 79.4-96wt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410415629.2A CN104225645A (en) | 2014-08-21 | 2014-08-21 | Microbial preparation for purifying animal breeding environment and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410415629.2A CN104225645A (en) | 2014-08-21 | 2014-08-21 | Microbial preparation for purifying animal breeding environment and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104225645A true CN104225645A (en) | 2014-12-24 |
Family
ID=52215114
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410415629.2A Pending CN104225645A (en) | 2014-08-21 | 2014-08-21 | Microbial preparation for purifying animal breeding environment and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104225645A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104431455A (en) * | 2014-11-20 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Ammonia removal bacterium liquid of chicken house |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102475897A (en) * | 2010-11-19 | 2012-05-30 | 江苏邦德生物科技发展有限公司 | Biological antibacterial deodorant |
| CN107711885A (en) * | 2017-09-06 | 2018-02-23 | 山东绿神肥业有限公司 | A kind of foliage-spray preparation and its production method for preventing and treating Wheat lodging |
-
2014
- 2014-08-21 CN CN201410415629.2A patent/CN104225645A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102475897A (en) * | 2010-11-19 | 2012-05-30 | 江苏邦德生物科技发展有限公司 | Biological antibacterial deodorant |
| CN107711885A (en) * | 2017-09-06 | 2018-02-23 | 山东绿神肥业有限公司 | A kind of foliage-spray preparation and its production method for preventing and treating Wheat lodging |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104431455A (en) * | 2014-11-20 | 2015-03-25 | 镇江拜因诺生物科技有限公司 | Ammonia removal bacterium liquid of chicken house |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100375637C (en) | Biological deodorization and purification agent, and its use | |
| CN103964935B (en) | Organic active fertilizer | |
| CN108456650B (en) | Compound microbial deodorant bacterial agent and preparation method and application thereof | |
| CN100425690C (en) | Fast harmless biological treating process of animal excrement and manure water | |
| CN110498712B (en) | Biological organic fertilizer and preparation method thereof | |
| CN106591199B (en) | High-efficiency leavening agent for livestock and poultry manure and leavening method thereof | |
| CN106047772A (en) | Composite microbial agent for treating high-ammonia-nitrogen sewage and preparation method and application thereof | |
| CN102258796A (en) | Microbial deodorant and preparation method thereof | |
| KR20160001411A (en) | Environment-friendly deodorant using effective micro-organisms | |
| CN101433730A (en) | Treatment agent for domestic refuse containing plant vinegar liquor and uses thereof | |
| CN107162652B (en) | Odorless livestock and poultry manure organic fertilizer and preparation method and application thereof | |
| CN101284737A (en) | Manure composting deodorization treating compositions | |
| CN104862298A (en) | Composite microbial culture starter and preparation method thereof | |
| CN109182157A (en) | A kind of composite bacteria preparation and preparation method thereof improving breeding environment | |
| KR101296613B1 (en) | A microbe deodorizer | |
| CN102813948A (en) | Preparation method of composite biological deodorant | |
| CN110894102A (en) | Method for purifying malodorous substances in livestock breeding environment | |
| CN108794189A (en) | A kind of preparation method of NEW TYPE OF COMPOSITE bio-feritlizer | |
| RU2347808C1 (en) | Biopreparation from effective microbes for breaking down organic wastes | |
| CN107723254A (en) | A kind of biological organic fertilizer zymophyte for discarded object fermentation process | |
| CN108531412A (en) | A kind of preparation method of ECMD Efficient Rings control microbial deodorant | |
| CN107916241A (en) | The composite bacteria agent and its application of fortimicin concentration and drug resistant gene in a kind of reduction composting process | |
| KR20030020233A (en) | Liquid fertilizer manufacturing method of high concentrated organic wastes. | |
| CN103656712A (en) | Seaweed biological deodorant and preparation method thereof | |
| CN104225645A (en) | Microbial preparation for purifying animal breeding environment and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20141224 |
|
| RJ01 | Rejection of invention patent application after publication |