CN104223052A - Spiral seaweed phycocyanin micro-capsule and preparation method thereof - Google Patents
Spiral seaweed phycocyanin micro-capsule and preparation method thereof Download PDFInfo
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- CN104223052A CN104223052A CN201310243071.XA CN201310243071A CN104223052A CN 104223052 A CN104223052 A CN 104223052A CN 201310243071 A CN201310243071 A CN 201310243071A CN 104223052 A CN104223052 A CN 104223052A
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- phycocyanin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/185—Vegetable proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
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Abstract
The invention discloses a preparation method of a spiral seaweed phycocyanin micro-capsule. The preparation method comprises the following steps: 1 weighing 100-300 parts by weight of spiral seaweed phycocyanin, 150-350 parts by weight of arabic gum, 200-250 parts by weight of ethyl acetate, 500-700 parts by weight of liquid paraffin and 150-200 parts by weight of distilled water; 2 dissolving the arabic gum in the distilled water, stirring until the Arabic gum is dissolved, adding 100-300 parts by weight of spiral seaweed phycocyanin, and stirring at a temperature of 200-250r/min for 20-30min to ensure that the spiral seaweed phycocyanin is dissolved for later use; and 3 adding a solution obtained in the step 2 into the liquid paraffin which is preheated to 60-70 DEG C, stirring for dissolving, adding into the ethyl acetate which is pre-cooled to 5-8 DEG C, stirring at a temperature of 200-250r/min for 20-30min, and drying the obtained micro-capsule under a reduced pressure to obtain the spiral seaweed phycocyanin micro-capsule. The preparation method is simple in process, is capable of effectively maintaining the stability of the spiral seaweed phycocyanin, and is beneficial to popularization and application of the spiral seaweed phycocyanin.
Description
Technical field
This technology relates to a kind of micro-capsule and preparation method thereof, belongs to technical field of food biotechnology, particularly a kind of spirulina phycocyanin micro-capsule and preparation method thereof.
Background technology
Spirulina (Spirulina) is thread many cells blue-green algae, and the intracellular amino acid classes of its frond is complete and protein content is high, because being paid close attention to widely containing the multiple nutriment useful to human body.Phycocyanin is the primary protein component in spirulina, clinical study results shows, phycocyanin has raising body immunity, promote animal blood cytothesis, suppress the effects such as some cancer cell, in radioresistance, antifatigue, scavenging free radicals etc., also have certain physiologically active, therefore the research of spirulina phycocyanin has become the focus that spirulina albuminoid is studied simultaneously.Because spirulina phycocyanin is unstable, easy in inactivation sex change, oxidation deterioration, affects the application of spirulina phycocyanin.Because spirulina phycocyanin exists above-mentioned a series of shortcoming deficiency, bring very large difficulty to clinical practice, microcapsule technology is one of method of the above-mentioned shortcoming deficiency overcoming spirulina phycocyanin.
Wang Weijie etc. the 09th phase in 2010 " grain and oil processing " delivered " preparation technology of spirulina phycocyanin micro-capsule ", the document discloses with shitosan and sodium alginate as capsule material, micro-capsule is carried out to spirulina phycocyanin (C-PC), the optimum process condition preparing mosanom shitosan C-PC micro-capsule is determined: the deacetylation of shitosan is 74% by test, rotating speed 600r/min, mixing time 30min, syringe needle distance coagulation bath liquid level 10cm, rate of addition 30/min, sodium alginate concentration 2.5% (w/v), chitosan concentration 0.6% (w/v), the mass ratio 1: 4 (w/w) of C-PC and sodium alginate, CaCl
2concentration 2.0% (w/v).But this process is relatively loaded down with trivial details, and is not suitable for industrialized production.
Summary of the invention
An object of the present invention is to provide a kind of stay-in-grade spirulina phycocyanin micro-capsule.Further aim of the present invention is to provide the simple spirulina phycocyanin microcapsule preparation method of a kind of technique.
The technical solution adopted for the present invention to solve the technical problems is,
A preparation method for spirulina phycocyanin micro-capsule, comprises the steps:
1) spirulina phycocyanin 100-300 part is taken by weight, Arabic gum 150-350 part, ethyl acetate 200-250 part, atoleine 500-700 part, distilled water 150-200 part;
2) described Arabic gum is dissolved in distilled water, is stirred to dissolving, then add spirulina phycocyanin 100-300 part, stir at 200-250r/min and make it dissolve in 20-30 minute, for subsequent use;
3) by step 2) gained solution joins and has been heated in the atoleine of 60-70 DEG C in advance, stirring and dissolving, then add and be cooled in the ethyl acetate of 5-8 DEG C in advance, 200-250r/min stirs 20-30 minute, by gained micro-capsule drying under reduced pressure.
Preferably, in order to better obtain better micro-capsule, described spirulina phycocyanin crosses 120 mesh sieves.
Preferably, described step 1) gets spirulina phycocyanin 200 parts, Arabic gum 250 parts, 200 parts, ethyl acetate, atoleine 600 parts, distilled water 150 parts.Described step 2) described Arabic gum is dissolved in distilled water, be stirred to dissolving, then add spirulina phycocyanin 100-300 part, stir at 200r/min and make it dissolve in 20 minutes, for subsequent use.Described step 3) is by step 2) gained solution joins and has been heated in the atoleine of 60 DEG C in advance, stirring and dissolving, then add and be cooled in the ethyl acetate of 5 DEG C in advance, 250r/min stirs 30 minutes, by gained micro-capsule drying under reduced pressure.
Preparation method's technique of the present invention is simple, and cost of material is low, is very applicable to industrialized production, and obtained spirulina phycocyanin micro-capsule steady quality, can long storage periods consistency.
Detailed description of the invention
The extracting method of spirulina phycocyanin of the present invention is as follows:
The extraction of spirulina phycocyanin: (1) clasmatosis: take 0.5g spirulina powder, adding pH with the ratio of 1g/200ml is in the phosphate buffer of the 0.01mol/L of 7.0, multigelation (3 times) carries out fragmentation to frond cell, by the spirulina cells suspension of fragmentation in the centrifugal 20min of 4000r/min, topple over the crude extract that supernatant can obtain containing phycocyanin.(2) aqueous two-phase extraction: adding concentration in crude extract is 12%PEG4000,15%Na2SO4, the double-aqueous phase system of 1%KCl is for extracting phycocyanin in the broken liquid of spirulina cells, abundant vibration makes into phase substance dissolves, two-phase laminated flow after leaving standstill, phase solution in extraction, namely freeze drying obtains spirulina phycocyanin finished product.
Below in conjunction with preferred embodiment, to according to detailed description of the invention provided by the invention, details are as follows:
The preparation of embodiment 1 spirulina phycocyanin micro-capsule
A preparation for spirulina phycocyanin micro-capsule, carries out: (every part by 10g) as follows
(1) 120 mesh sieve spirulina phycocyanin 100 parts was taken by weight, Arabic gum 150 parts, 200 parts, ethyl acetate, atoleine 700 parts, distilled water 250 parts;
(2) described Arabic gum is dissolved in distilled water, is stirred to dissolving, add spirulina phycocyanin 150 parts, stir at 200r/min and make it dissolve in 20 minutes;
(3) be added in the atoleine being heated to 60 DEG C in advance by step (2) gained solution, stirring and dissolving, add in the ethyl acetate being cooled to 5 DEG C, 250r/min stirs 30 minutes, by gained micro-capsule drying under reduced pressure and get final product.
The preparation of embodiment 2 spirulina phycocyanin micro-capsule
A preparation for spirulina phycocyanin micro-capsule, carries out: (every part by 10g) as follows
(1) 120 mesh sieve spirulina phycocyanin 200 parts was taken by weight, Arabic gum 250 parts, 200 parts, ethyl acetate, atoleine 600 parts, distilled water 150 parts;
(2) described Arabic gum is dissolved in distilled water, is stirred to dissolving, add spirulina phycocyanin 150 parts, stir at 200r/min and make it dissolve in 20 minutes;
(3) be added in the atoleine being heated to 60 DEG C in advance by (2) gained solution, stirring and dissolving, add in the ethyl acetate being cooled to 5 DEG C, 250r/min stirs 30 minutes, by gained micro-capsule drying under reduced pressure and get final product.
The preparation of embodiment 3 spirulina phycocyanin micro-capsule
A preparation for spirulina phycocyanin micro-capsule, carries out: (every part by 10g) as follows
(1) 120 mesh sieve spirulina phycocyanin 300 parts was taken by weight, Arabic gum 350 parts, 200 parts, ethyl acetate, atoleine 500 parts, distilled water 250 parts;
(2) described Arabic gum is dissolved in distilled water, is stirred to dissolving, add spirulina phycocyanin 250 parts, stir at 200r/min and make it dissolve in 20 minutes;
(3) be added in the atoleine being heated to 60 DEG C in advance by (2) gained solution, stirring and dissolving, add in the ethyl acetate being cooled to 5 DEG C, 250r/min stirs 30 minutes, by gained micro-capsule drying under reduced pressure and get final product.
The stability test of embodiment 4 spirulina phycocyanin micro-capsule
The spirulina phycocyanin of the spirulina phycocyanin micro-capsule obtained with embodiment 1 ~ 3 method and non-microencapsulation, do parallel test and average.Place under temperature (40 ± 2) DEG C, humidity (75 ± 5) % condition, respectively at 1,3,6,9,12 month sample analysis with within 0 month, investigate data and compare, measure the light absorption value at 620nm place, result shows, continuous placement 12 months, the feature light absorption value of the spirulina phycocyanin of microencapsulation does not have significant change, highly stable with spirulina phycocyanin prepared by micro-capsule.
Study on the stability result of the test (temperature (40 ± 2) DEG C, RH (75 ± 5) %, n=10)
Claims (6)
1. a preparation method for spirulina phycocyanin micro-capsule, comprises following method step:
1) spirulina phycocyanin 100-300 part is taken by weight, Arabic gum 150-350 part, ethyl acetate 200-250 part, atoleine 500-700 part, distilled water 150-200 part;
2) described Arabic gum is dissolved in distilled water, is stirred to dissolving, then add spirulina phycocyanin 100-300 part, stir at 200-250r/min and make it dissolve in 20-30 minute, for subsequent use;
3) by step 2) gained solution joins and has been heated in the atoleine of 60-70 DEG C in advance, stirring and dissolving, then add and be cooled in the ethyl acetate of 5-8 DEG C in advance, 200-250r/min stirs 20-30 minute, by gained micro-capsule drying under reduced pressure.
2. the preparation method of spirulina phycocyanin micro-capsule as claimed in claim 1, is characterized in that: described spirulina phycocyanin crosses 120 mesh sieves.
3. the preparation method of spirulina phycocyanin micro-capsule as claimed in claim 1, is characterized in that: described step 1) gets spirulina phycocyanin 200 parts, Arabic gum 250 parts, 200 parts, ethyl acetate, atoleine 600 parts, distilled water 150 parts.
4. the preparation method of spirulina phycocyanin micro-capsule as claimed in claim 1, it is characterized in that: described step 2) described Arabic gum is dissolved in distilled water, be stirred to dissolving, then add spirulina phycocyanin 100-300 part, stirring at 200r/min makes it dissolve in 20 minutes, for subsequent use.
5. the preparation method of spirulina phycocyanin micro-capsule as claimed in claim 1, it is characterized in that: described step 3) is by step 2) gained solution joins and has been heated in the atoleine of 60 DEG C in advance, stirring and dissolving, add again and be cooled in the ethyl acetate of 5 DEG C in advance, 250r/min stirs 30 minutes, by gained micro-capsule drying under reduced pressure.
6. a spirulina phycocyanin micro-capsule is obtained by the either method described in Claims 1 to 5.
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| CN201310243071.XA CN104223052A (en) | 2013-06-19 | 2013-06-19 | Spiral seaweed phycocyanin micro-capsule and preparation method thereof |
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| CN201310243071.XA CN104223052A (en) | 2013-06-19 | 2013-06-19 | Spiral seaweed phycocyanin micro-capsule and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108013449A (en) * | 2017-11-02 | 2018-05-11 | 天津大学 | A kind of preparation method of composite microcapsule using spirulina as main wall material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108013449A (en) * | 2017-11-02 | 2018-05-11 | 天津大学 | A kind of preparation method of composite microcapsule using spirulina as main wall material |
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Application publication date: 20141224 |