CN101933958B - Preparation method of kiwi polyphenol microcapsules - Google Patents
Preparation method of kiwi polyphenol microcapsules Download PDFInfo
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- CN101933958B CN101933958B CN 201010271827 CN201010271827A CN101933958B CN 101933958 B CN101933958 B CN 101933958B CN 201010271827 CN201010271827 CN 201010271827 CN 201010271827 A CN201010271827 A CN 201010271827A CN 101933958 B CN101933958 B CN 101933958B
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- chitosan
- sodium alginate
- kiwi polyphenol
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Abstract
The invention relates to a preparation method of kiwi polyphenol microcapsules, which comprises the steps of weighing chitosan, dissolving in acetic acid solution, regulating pH to pH3.0-6.0, adding calcium chloride into the solution, and fully stirring till the complete dissolution; adding kiwi polyphenols into sodium alginate solution with the concentration of 0.8-3.0%(w/v), and fully stirring till the complete dissolution; and uniformly adding mixed solution of the sodium alginate and the kiwi polyphenols into the chitosan solution through micro-holes under stirring at the constant temperature of 25-40 DEG C, standing, filtering, then carrying out treatment with borax buffer solution with pH3, washing, drying and then obtaining the kiwi polyphenol microcapsules. The prepared kiwi polyphenol microcapsules can improve the bioavailability of the kiwi polyphenols, increase the stability of the kiwi polyphenols, lead the drug loading rate of the kiwi polyphenol microcapsules to achieve 23.4%, and lead the total release rate in simulated gastric and intestinal fluids to achieve 85% at 4 hours.
Description
Technical field
The present invention relates to a kind of preparation method of microcapsule, particularly relate to a kind of preparation method of kiwi polyphenol microcapsules.
Background technology
Micro encapsulation is that solid, liquid, gas attitude material is embedded in the capsule of small, semi permeability or sealing, the technology that content is discharged with controlled speed under given conditions.The capsule of this small sealing is microcapsule, and the process of parcel is micro encapsulation.Microcapsule generally is spherical.The main purpose of micro encapsulation comprises can control release, improves the bioavailability of effective ingredient; Change the physical property of medicine, increase the stability of medicine; Reduce toxic and side effects, change the character of medicine, reduce the incompatibility between the compound preparation Chinese medicine; Cover smell and zest etc.
The principle of preparation microcapsule has multiple, wherein by the polyelectrolyte complex principle prepare microcapsule be adopt more a kind of.The remarkable advantage for preparing microcapsule by this principle is that preparation process is gentle, and the bioactive substance of microencapsulation loss of activity in preparation process seldom or not loses.Preparing the selected yin, yang ion polyelectrolyte material of microcapsule by this principle also has multiple.
At present, microcapsule prepares material and mainly contains tens of kinds of synthetic/semi-synthetic and natural polymers etc.Polysaccharide (such as sodium alginate and chitosan) and protein (such as albumin and gelatin) are the representatives of natural material, usually nontoxic, immunogenicity is low.
Chitosan is comprised of glucosamine and two kinds of aminopolysaccharides of 2-Acetamido-2-deoxy-D-glucose, and chitosan can be dissolved in diluted acid, can form the cation amino polysaccharide.The hydroxyl that enriches on its strand and amino make it be easy to carry out chemical modification and give several functions, are used widely in fields such as medicine, agricultural, light textile, daily use chemicals.As a kind of biological polysaccharide polymer material, it has nontoxic, and is safe and reliable, is easy to biodegradation, good biocompatibility, the characteristics such as free from environmental pollution.
Sodium alginate is a kind of polysaccharide line style natural high molecular substance, is comprised of D-MANNOSE aldehydic acid segment and L-guluronic acid segment alternating structure, owing on the strand of sodium alginate a large amount of carboxyls being arranged, it is a kind of anionic polymer.Its aqueous solution has colloid property, thickening property, stability, emulsibility and film property.
Chitosan cation amino and sodium alginate anion attract by positive and negative charge, produce instantaneous gelling, form the polyelectrolyte semipermeable membrane, the kiwi polyphenol embedding can be improved the bioavailability of kiwi polyphenol, increase the stability of kiwi polyphenol.
Summary of the invention
The preparation method that the purpose of this invention is to provide a kind of kiwi polyphenol microcapsules, in this way the kiwi polyphenol microcapsules of preparation can improve the bioavailability of kiwi polyphenol, increase the stability of kiwi polyphenol, the carrying drug ratio of resulting kiwi polyphenol microcapsules reaches 23.4%, and the total release rate in simulated gastric fluid and the intestinal juice reached 85% in 4 hours.
For achieving the above object, the present invention adopts following technical scheme: a kind of preparation method of kiwi polyphenol microcapsules, it is characterized in that, taking by weighing deacetylation and be 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v), regulate pH value to pH3.0～6.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), and slight fever is stirred well to dissolve complete.Add 5～25% kiwi polyphenol (w/v) in concentration 0.8～3.0% (w/v) sodium alginate soln, be stirred well to dissolve complete.Under 25～40 ℃ of constant temperature stir, evenly add in chitosan solution by micropore sodium alginate and kiwi polyphenol mixed solution, the speed that adds is 0.5～3mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is processed, and washing obtains kiwi polyphenol microcapsules after the drying.
Deacetylation is that 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v).
The chitosan solution pH value transfers to pH3.0～6.0, and calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), and slight fever is stirred well to dissolve complete.
0.8 add 5～25% kiwi polyphenol (w/v) in～3.0% (w/v) sodium alginate soln, be stirred well to dissolve complete.
Under 25～40 ℃ of constant temperature stir, sodium alginate and kiwi polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 0.5～3mL/min, leaves standstill 5～10 minutes, filters.
The borax buffer solution of pH3 is processed, and washing obtains kiwi polyphenol microcapsules after the drying.
The specific embodiment
Embodiment 1: take by weighing deacetylation and be 75% chitosan and be dissolved in acetum and make chitosan solution concentration reach 0.5% (w/v), regulate pH value to pH3.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 2% (w/v), and slight fever is stirred well to dissolve complete.The kiwi polyphenol (w/v) of adding 15% is stirred well to dissolve complete in concentration 1% (w/v) sodium alginate soln.Under 25 ℃ of constant temperature stir, sodium alginate and kiwi polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 0.5mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is processed, washing obtains kiwi polyphenol microcapsules after the drying.
Embodiment 2: take by weighing deacetylation and be 80% chitosan and be dissolved in acetum and make chitosan solution concentration reach 1.0% (w/v), regulate pH value to pH4.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 3% (w/v), and slight fever is stirred well to dissolve complete.The kiwi polyphenol (w/v) of adding 10% is stirred well to dissolve complete in concentration 2.0% (w/v) sodium alginate soln.Under 30 ℃ of constant temperature stir, sodium alginate and kiwi polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 2mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is processed, washing obtains kiwi polyphenol microcapsules after the drying.
Embodiment 3: take by weighing deacetylation and be 85% chitosan and be dissolved in acetum and make chitosan solution concentration reach 1.5% (w/v), regulate pH value to pH5.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1.0% (w/v), and slight fever is stirred well to dissolve complete.The kiwi polyphenol (w/v) of adding 25% is stirred well to dissolve complete in concentration 3.0% (w/v) sodium alginate soln.Under 35 ℃ of constant temperature stir, sodium alginate and kiwi polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 3mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is processed, washing obtains kiwi polyphenol microcapsules after the drying.
Claims (1)
1. the preparation method of a kiwi polyphenol microcapsules, it is characterized in that, taking by weighing deacetylation and be 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v), regulate pH value to pH3.0～6.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), slight fever is stirred well to dissolve complete.Add 5～25% kiwi polyphenol (w/v) in concentration 0.8～3.0% (w/v) sodium alginate soln, be stirred well to dissolve complete.Under 25～40 ℃ of constant temperature stir, evenly add in chitosan solution by micropore sodium alginate and kiwi polyphenol mixed solution, the speed that adds is 0.5～3mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is processed, and washing obtains kiwi polyphenol microcapsules after the drying.
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| CN 201010271827 CN101933958B (en) | 2010-09-03 | 2010-09-03 | Preparation method of kiwi polyphenol microcapsules |
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| CN 201010271827 CN101933958B (en) | 2010-09-03 | 2010-09-03 | Preparation method of kiwi polyphenol microcapsules |
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| CN101933958A CN101933958A (en) | 2011-01-05 |
| CN101933958B true CN101933958B (en) | 2013-03-27 |
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| CN 201010271827 Expired - Fee Related CN101933958B (en) | 2010-09-03 | 2010-09-03 | Preparation method of kiwi polyphenol microcapsules |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103783349A (en) * | 2014-02-19 | 2014-05-14 | 江苏大学 | Process for preparing honeycomb polyphenol extractive microcapsule by adopting spray drying method |
| CN105724526A (en) * | 2016-02-22 | 2016-07-06 | 天津农学院 | Making method of leisure blood-sugar-reducing and health-care biscuits taking oats as substrate |
| CN106690154B (en) * | 2016-11-22 | 2020-10-09 | 张海生 | Processing method of red date polyphenol microcapsule food |
| CN111514097B (en) * | 2020-05-14 | 2022-02-11 | 燕山大学 | Preparation method of pH-responsive nano hydrogel of walnut shell polyphenol |
| CN112777673A (en) * | 2020-12-25 | 2021-05-11 | 长春工业大学 | Method for reducing concentration of heavy metal iron ions in water |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383819A (en) * | 2002-05-17 | 2002-12-11 | 天津市园艺工程研究所 | Protocyanidin slow release capsule |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383819A (en) * | 2002-05-17 | 2002-12-11 | 天津市园艺工程研究所 | Protocyanidin slow release capsule |
Non-Patent Citations (2)
| Title |
|---|
| 从猕猴桃中提取多酚的研究;周跃勇等;《食品研究与开发》;20070331;第28卷(第3期);56-60 * |
| 周跃勇等.从猕猴桃中提取多酚的研究.《食品研究与开发》.2007,第28卷(第3期),56-60. |
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