CN101933958A - Preparation method of kiwi polyphenol microcapsules - Google Patents
Preparation method of kiwi polyphenol microcapsules Download PDFInfo
- Publication number
- CN101933958A CN101933958A CN2010102718278A CN201010271827A CN101933958A CN 101933958 A CN101933958 A CN 101933958A CN 2010102718278 A CN2010102718278 A CN 2010102718278A CN 201010271827 A CN201010271827 A CN 201010271827A CN 101933958 A CN101933958 A CN 101933958A
- Authority
- CN
- China
- Prior art keywords
- solution
- kiwi
- actinidiae chinensis
- fructus actinidiae
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicinal Preparation (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
The invention relates to a preparation method of kiwi polyphenol microcapsules, which comprises the steps of weighing chitosan, dissolving in acetic acid solution, regulating pH to pH3.0-6.0, adding calcium chloride into the solution, and fully stirring till the complete dissolution; adding kiwi polyphenols into sodium alginate solution with the concentration of 0.8-3.0%(w/v), and fully stirring till the complete dissolution; and uniformly adding mixed solution of the sodium alginate and the kiwi polyphenols into the chitosan solution through micro-holes under stirring at the constant temperature of 25-40 DEG C, standing, filtering, then carrying out treatment with borax buffer solution with pH3, washing, drying and then obtaining the kiwi polyphenol microcapsules. The prepared kiwi polyphenol microcapsules can improve the bioavailability of the kiwi polyphenols, increase the stability of the kiwi polyphenols, lead the drug loading rate of the kiwi polyphenol microcapsules to achieve 23.4%, and lead the total release rate in simulated gastric and intestinal fluids to achieve 85% at 4 hours.
Description
Technical field
The present invention relates to a kind of preparation method of microcapsule, particularly relate to a kind of preparation method of Fructus actinidiae chinensis polyphenol microcapsule.
Background technology
Micro encapsulation is that solid, liquid, gas attitude material is embedded in the capsule of small, semi permeability or sealing, the technology that content is discharged with controlled speed under given conditions.The capsule of this small sealing is a microcapsule, and the process of parcel is a micro encapsulation.Microcapsule generally is globular.The main purpose of micro encapsulation comprises can sustained release, improves the bioavailability of effective ingredient; Change the physical property of medicine, increase stability of drug; Reduce toxic and side effects, change the character of medicine, reduce the incompatibility between the compound preparation Chinese medicine; Cover smell and zest etc.
The principle of preparation microcapsule has multiple, wherein by the polyelectrolyte complex principle prepare microcapsule be adopt more a kind of.The remarkable advantage for preparing microcapsule by this principle is the preparation process gentleness, and the bioactive substance of microencapsulation loss of activity in preparation process seldom or not loses.Preparing the selected yin, yang ion polyelectrolyte material of microcapsule by this principle also has multiple.
At present, microcapsule prepares material and mainly contains tens of kinds of synthetic/semi-synthetic and natural polymers etc.Polysaccharide (as sodium alginate and chitosan) and protein (as albumin and gelatin) are the representatives of natural material, and is nontoxic usually, immunogenicity is low.
Chitosan is made up of glucosamine and two kinds of aminopolysaccharides of N-acetylglucosamine, and chitosan can be dissolved in diluted acid, can form the cation amino polysaccharide.Hydroxyl that enriches on its strand and amino make it be easy to carry out chemical modification and give multiple function, are used widely in fields such as medicine, agricultural, light textile, daily use chemicals.As a kind of biological polysaccharide polymer material, it has nontoxic, and is safe and reliable, is easy to biodegradation, good biocompatibility, characteristics such as free from environmental pollution.
Sodium alginate is a kind of polysaccharide line style natural high molecular substance, is made up of D-mannuronic acid segment and L-guluronic acid segment alternating structure, owing on the strand of sodium alginate a large amount of carboxyls is arranged, it is a kind of anionic polymer.Its aqueous solution has colloid property, thickening property, stability, emulsibility and film property.
Chitosan cation amino and sodium alginate anion attract by positive and negative charge, produce instantaneous gelling, form the polyelectrolyte semipermeable membrane, the embedding of Fructus actinidiae chinensis polyphenol can be improved the bioavailability of Fructus actinidiae chinensis polyphenol, increase the stability of Fructus actinidiae chinensis polyphenol.
Summary of the invention
The preparation method that the purpose of this invention is to provide a kind of Fructus actinidiae chinensis polyphenol microcapsule, in this way Zhi Bei Fructus actinidiae chinensis polyphenol microcapsule can improve the bioavailability of Fructus actinidiae chinensis polyphenol, increase the stability of Fructus actinidiae chinensis polyphenol, the carrying drug ratio of resulting Fructus actinidiae chinensis polyphenol microcapsule reaches 23.4%, and the total release rate in simulated gastric fluid and the intestinal juice reached 85% in 4 hours.
For achieving the above object, the present invention adopts following technical scheme: a kind of preparation method of Fructus actinidiae chinensis polyphenol microcapsule, it is characterized in that, taking by weighing deacetylation and be 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v), regulate pH value to pH3.0～6.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), and slight fever is stirred well to dissolving fully.Add 5～25% Fructus actinidiae chinensis polyphenol (w/v) in concentration 0.8～3.0% (w/v) sodium alginate soln, be stirred well to dissolving fully.Under 25～40 ℃ of constant temperature stir, evenly add in chitosan solution by micropore sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution, the speed that adds is 0.5～3mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is handled, and washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
Deacetylation is that 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v).
The chitosan solution pH value transfers to pH3.0～6.0, and calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), and slight fever is stirred well to dissolving fully.
0.8 add 5～25% Fructus actinidiae chinensis polyphenol (w/v) in～3.0% (w/v) sodium alginate soln, be stirred well to dissolving fully.
25～40 ℃ of constant temperature stir down, and sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution are evenly added in the chitosan solution by micropore, and the speed of adding is 0.5～3mL/min, leaves standstill 5～10 minutes, filters.
The borax buffer solution of pH3 is handled, and washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
The specific embodiment
Embodiment 1: take by weighing deacetylation and be 75% chitosan and be dissolved in acetum and make chitosan solution concentration reach 0.5% (w/v), regulate pH value to pH3.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 2% (w/v), and slight fever is stirred well to dissolving fully.The Fructus actinidiae chinensis polyphenol (w/v) of adding 15% in concentration 1% (w/v) sodium alginate soln is stirred well to dissolving fully.Under 25 ℃ of constant temperature stir, sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 0.5mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is handled, washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
Embodiment 2: take by weighing deacetylation and be 80% chitosan and be dissolved in acetum and make chitosan solution concentration reach 1.0% (w/v), regulate pH value to pH4.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 3% (w/v), and slight fever is stirred well to dissolving fully.The Fructus actinidiae chinensis polyphenol (w/v) of adding 10% in concentration 2.0% (w/v) sodium alginate soln is stirred well to dissolving fully.Under 30 ℃ of constant temperature stir, sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 2mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is handled, washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
Embodiment 3: take by weighing deacetylation and be 85% chitosan and be dissolved in acetum and make chitosan solution concentration reach 1.5% (w/v), regulate pH value to pH5.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1.0% (w/v), and slight fever is stirred well to dissolving fully.The Fructus actinidiae chinensis polyphenol (w/v) of adding 25% in concentration 3.0% (w/v) sodium alginate soln is stirred well to dissolving fully.Under 35 ℃ of constant temperature stir, sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution are evenly added in the chitosan solution by micropore, the speed of adding is 3mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is handled, washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
Claims (6)
1. the preparation method of a Fructus actinidiae chinensis polyphenol microcapsule, it is characterized in that, taking by weighing deacetylation and be 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v), regulate pH value to pH3.0～6.0, calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), slight fever is stirred well to dissolving fully.Add 5～25% Fructus actinidiae chinensis polyphenol (w/v) in concentration 0.8～3.0% (w/v) sodium alginate soln, be stirred well to dissolving fully.Under 25～40 ℃ of constant temperature stir, evenly add in chitosan solution by micropore sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution, the speed that adds is 0.5～3mL/min, left standstill 5～10 minutes, after the filtration, the borax buffer solution of pH3 is handled, and washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
2. preparation method as claimed in claim 1 is characterized in that, deacetylation is that 70%～85% chitosan is dissolved in acetum and makes chitosan solution concentration reach 0.3～1.5% (w/v).
3. preparation method as claimed in claim 1 is characterized in that the chitosan solution pH value transfers to pH3.0～6.0, and calcium chloride adds the concentration that makes calcium chloride in the above solution and reaches 1～5% (w/v), and slight fever is stirred well to dissolving fully.。
4. preparation method as claimed in claim 1 is characterized in that, adds 5～25% Fructus actinidiae chinensis polyphenol (w/v) in 0.8～3.0% (w/v) sodium alginate soln, is stirred well to dissolving fully.
5. preparation method as claimed in claim 1 is characterized in that, 25～40 ℃ of constant temperature stir down, evenly add in chitosan solution by micropore sodium alginate and Fructus actinidiae chinensis polyphenol mixed solution, the speed that adds is 0.5～3mL/min, leaves standstill 5～10 minutes, filters.
6. preparation method as claimed in claim 1 is characterized in that, the borax buffer solution of pH3 is handled, and washing obtains Fructus actinidiae chinensis polyphenol microcapsule after the drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010271827 CN101933958B (en) | 2010-09-03 | 2010-09-03 | Preparation method of kiwi polyphenol microcapsules |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010271827 CN101933958B (en) | 2010-09-03 | 2010-09-03 | Preparation method of kiwi polyphenol microcapsules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101933958A true CN101933958A (en) | 2011-01-05 |
| CN101933958B CN101933958B (en) | 2013-03-27 |
Family
ID=43387679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010271827 Expired - Fee Related CN101933958B (en) | 2010-09-03 | 2010-09-03 | Preparation method of kiwi polyphenol microcapsules |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101933958B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783349A (en) * | 2014-02-19 | 2014-05-14 | 江苏大学 | Process for preparing honeycomb polyphenol extractive microcapsule by adopting spray drying method |
| CN105724526A (en) * | 2016-02-22 | 2016-07-06 | 天津农学院 | Making method of leisure blood-sugar-reducing and health-care biscuits taking oats as substrate |
| CN106690154A (en) * | 2016-11-22 | 2017-05-24 | 张海生 | Processing method of red jujube polyphenol microcapsule food |
| CN111514097A (en) * | 2020-05-14 | 2020-08-11 | 燕山大学 | Preparation method of pH-responsive nano hydrogel of walnut shell polyphenol |
| CN112777673A (en) * | 2020-12-25 | 2021-05-11 | 长春工业大学 | Method for reducing concentration of heavy metal iron ions in water |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383819A (en) * | 2002-05-17 | 2002-12-11 | 天津市园艺工程研究所 | Protocyanidin slow release capsule |
-
2010
- 2010-09-03 CN CN 201010271827 patent/CN101933958B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383819A (en) * | 2002-05-17 | 2002-12-11 | 天津市园艺工程研究所 | Protocyanidin slow release capsule |
Non-Patent Citations (4)
| Title |
|---|
| 周跃勇等: "从猕猴桃中提取多酚的研究", 《食品研究与开发》, vol. 28, no. 3, 31 March 2007 (2007-03-31), pages 56 - 60 * |
| 宋春津等: "海藻酸钠和壳聚糖反应包裹化学紫外吸收剂的应用", 《天津化工》, vol. 21, no. 4, 31 July 2007 (2007-07-31), pages 22 - 24 * |
| 王勇等: "壳聚糖、海藻酸钠生物微胶囊的研究进展", 《生物工程进展》, vol. 19, no. 2, 28 February 1999 (1999-02-28) * |
| 王岸娜等: "猕猴桃多酚组分及稳定性研究", 《河南工业大学学报（自然科学版）》, vol. 29, no. 2, 29 February 2008 (2008-02-29), pages 43 - 46 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783349A (en) * | 2014-02-19 | 2014-05-14 | 江苏大学 | Process for preparing honeycomb polyphenol extractive microcapsule by adopting spray drying method |
| CN105724526A (en) * | 2016-02-22 | 2016-07-06 | 天津农学院 | Making method of leisure blood-sugar-reducing and health-care biscuits taking oats as substrate |
| CN106690154A (en) * | 2016-11-22 | 2017-05-24 | 张海生 | Processing method of red jujube polyphenol microcapsule food |
| CN111514097A (en) * | 2020-05-14 | 2020-08-11 | 燕山大学 | Preparation method of pH-responsive nano hydrogel of walnut shell polyphenol |
| CN112777673A (en) * | 2020-12-25 | 2021-05-11 | 长春工业大学 | Method for reducing concentration of heavy metal iron ions in water |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101933958B (en) | 2013-03-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105054070B (en) | A kind of Sophora viciifolia color glycosides crude extract and its microcapsules | |
| CN105494430B (en) | One kind carries silver-colored low-molecular weight chitoglycan complex microsphere antiseptic and preparation method thereof | |
| CN101933958B (en) | Preparation method of kiwi polyphenol microcapsules | |
| CN104140568B (en) | A kind of edible film with lasting anti-oxidation function and preparation method thereof and application | |
| CN102824332B (en) | Preparation method of antimicrobial peptide slow-release microcapsules | |
| CN101288662B (en) | Xanthophyll micro-capsule and its preparation method | |
| CN102784600B (en) | Preparation method of lutein microcapsule | |
| CN102090395A (en) | Abscisic acid-embedded chitosan nanoparticles and preparation method thereof | |
| CN112048080B (en) | Microcapsule-filled sodium alginate-based hydrogel and preparation method thereof | |
| IL164357A0 (en) | Polysaccharide capsules and methods of preparation | |
| CN105776179B (en) | A kind of water soluble quaternary ammonium salinization Nano carbon balls and preparation method and application | |
| Gao et al. | Recent advances in microfluidic-aided chitosan-based multifunctional materials for biomedical applications | |
| CN105286011A (en) | Soluble soybean polysaccharide-soybean protein-curcumin compound as well as preparation and application | |
| CN104472646B (en) | A kind of preparation method of Nisin bilayered microcapsules suitable for high temperature bakery | |
| CN105661543B (en) | A kind of stable type intestines sustained release tea polyphenols microcapsules and preparation method thereof | |
| CN102408496A (en) | In-situ crosslinked alginate hydrogels and preparation method thereof | |
| CN111956618B (en) | Preparation method and application of lignin-based antitumor drug carrier | |
| CN106905443B (en) | The preparation method and applications of guanidinated chitosan | |
| CN102293266A (en) | Method for preparing antioxidant fish oil microcapsule | |
| CN107519149A (en) | A kind of preparation method for the silica/CS/PAA nucleocapsid composite nano anti-biotic particles for loading triclosan | |
| CN102356838A (en) | Lutein micro-capsule preparation and preparation method thereof | |
| CN107252132B (en) | Casein-carragheen independently fills the preparation method and applications of capsule of nano | |
| Yang et al. | pH-sensitive chitosan–sodium phytate core–shell hollow beads and nanocapsules for the encapsulation of active ingredients | |
| CN102120169A (en) | Sodium iron chlorophyllin-chitosan/sodium alginate microcapsules and preparation method thereof | |
| CN104873467B (en) | A kind of preparation method of Biodegradable interpenetrating net polymer microballoon |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130327 Termination date: 20130903 |