CN104212820A - Enzyme with catalase activity and encoding gene of enzyme - Google Patents
Enzyme with catalase activity and encoding gene of enzyme Download PDFInfo
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- CN104212820A CN104212820A CN201410469853.XA CN201410469853A CN104212820A CN 104212820 A CN104212820 A CN 104212820A CN 201410469853 A CN201410469853 A CN 201410469853A CN 104212820 A CN104212820 A CN 104212820A
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Abstract
The invention aims to provide an enzyme with catalase activity and an encoding gene of the enzyme. According to the enzyme, the gene is transformed into Aspergillus niger by using a gene engineering technological method, and is constructed to obtain a recombination strain for efficiently expressing the enzyme so as to facilitate the industrial production and wide application of the enzyme. The amino acid sequence of the enzyme is SEQ ID NO: 1, the optimal operation temperature of the enzyme is 60 DEG C, and the optimal operation pH value of the enzyme is 6.0; the enzyme provided by the invention can be used for effectively catalyzing hydrogen peroxide to decompose into water and oxygen. When H2O2 is treated by using the enzyme, H2O2 can be completely decomposed in 15 minutes, and H2O2 has the performance superior to that of commercially-available products, so that the enzyme provided by the invention can be widely applied to the industrial production of foods, spinning, medicines, papermaking and the like, and has wide application prospects.
Description
Technical field
The invention belongs to functional gene triage techniques field, be specifically related to a kind of enzyme and encoding gene thereof with catalase activity.
Background technology
Catalase (Hydrogen Peroxidase) claims again catalase (Catalase is called for short CAT), is the enzyme of a class catalytic substrate redox reaction, and its major function is that catalyzing hydrogen peroxide resolves into oxygen G&W.CAT is distributed widely in animal, plant tissue and in the anaerobion of most aerobic bacterias and minority.Animal livers, red corpuscle, plant chloroplast and actinomycetes, bacterium, fungi also contain CAT, wherein in mammalian tissues, CAT content difference is larger, liver and reticular tissue content are respectively the highest and minimum, CAT is mainly that the form of being combined as plastosome and peroxidase precursor with organoid exists in above-mentioned cell tissue, in red corpuscle, with soluble state, exists.The character gap of the CAT of different sources is larger, poor from the thermostability of the CAT that obtains in animals and plants and human body; And the stability of microbe-derived CAT shows better relatively, in addition, microorganism also has easy cultivation, originate wide, with short production cycle and low cost and other advantages.
The catalase main source animals and plants of domestic market are extracted, and from microorganism cells, extract also more.The separation and purification from genetic engineering bacterium expression product such as calendar year 2001 Liao Wenjun obtains catalase.In the strong bacterial cell disruption liquid waiting from recombination bacillus coli is cultivated of Wang Fan, through thermal treatment, ammonium sulfate precipitation, DEAE-SephadexA-50 ion exchange chromatography, HiPrep16/10Phenyl hydrophobic interaction chromatography, Superdex200HR10/30 gel chromatography obtain electrophoretically pure catalase after extracting.The purifying from the low haloalkaliphilic Bacillus of a strain such as Zhang Xinqi in 2005 obtains a kind of hydrogen peroxidase through fermenting.TC Dodge KM Huffmans in 2009 etc. are expressed the catR gene from aspergillus niger in Trichodermareesei, obtain with catR in aspergillus niger and express the hydrogen peroxide production of enzyme (China Patent No. 101960006) of comparing raising.Chen in 2011; Jian etc. make fermented liquid catalase activity reach 19700U/mL (U.S. Patent number 8,722,364) by add Sodium hexametaphosphate 99 in fermentation of bacillus.Qu Ling in 2013 etc. carry out recombinant expressed and purifying to the catalase of helicobacter pylori, through the recombinant protein purity of affinity chromatography, are about 85%.Therefore, microorganism catalase will become the important directions of catalase industrialization development, utilizes genetic engineering technique means to develop the catalase bacterial strain that novel active is high and has important using value.
Summary of the invention:
The object of this invention is to provide a kind of enzyme and encoding gene thereof with catalase activity.The present invention adopts genetic engineering technique means that the encoding gene of this enzyme is transformed in aspergillus niger (Aspergillus niger), builds and has obtained the recombinant bacterial strain of high this enzyme of efficient expression, thereby be conducive to realize suitability for industrialized production and the widespread use of this enzyme.
One aspect of the present invention provides a kind of gene, the enzyme that described genes encoding is following:
1) its aminoacid sequence is SEQ ID NO:1;
2) 1) in amino acid on replace, lack or add that one or several amino acid obtains, have 1) in the activity of enzyme, by 1) derivative enzyme.
Above-mentioned gene is separated from Gliocladium roseum (Gliocladium roseum).
Said gene, its a kind of nucleotide sequence is SEQ ID NO:2.
The invention provides a kind of expression vector, the nucleotide sequence that it comprises said gene.
The invention provides a kind of expression host cell, it carries above-mentioned expression vector.
Above-mentioned expression host cell is aspergillus niger (Aspergillus niger).
The present invention also provides a kind of enzyme, and its aminoacid sequence is SEQ ID NO:1.
The application of above-mentioned enzyme in degraded hydrogen peroxide.
Enzyme of the present invention has catalase activity, and its optimum temperature is 60 ℃, and the suitableeest action pH is 6.0, effectively catalysis H
2o
2decompose and generate water and oxygen.Utilize enzyme of the present invention to process H
2o
2, during 15min, be about to H
2o
2all decompose; And commercially available catalase described in control group, when 20min, ability is by H
2o
2all decompose, and it is at 5min, 10min, during 15min to H
2o
2decomposition effect all lower than catalase of the present invention.Enzyme performance of the present invention is better than commercially available prod, therefore can apply to widely the industrial production such as food, weaving, medicine, papermaking, has a extensive future.
Accompanying drawing explanation
Fig. 1: the recombinant expression plasmid pGm-2092 collection of illustrative plates that the present invention builds.
Fig. 2: positive transformant aspergillus niger ZL-3 fermented liquid supernatant SDS-PAGE electrophorogram, wherein the protein band at arrow indication 87.2KD place is enzyme of the present invention.
Fig. 3: the enzyme operative temperature of separation of the present invention-enzyme graphic representation alive.
Fig. 4: the enzyme action pH of separation of the present invention-enzyme graphic representation alive.
Embodiment
Below in conjunction with example, method of the present invention is described further.But example only limits to explanation, is not limited to this.The experimental technique of unreceipted actual conditions in the following example, conventionally condition routinely, condition described in the < < molecular cloning experiment guide > > writing as J. Pehanorm Brooker (Sambrook) etc., or the condition of advising according to manufacturer operation.Those skill in the art related can understand better and grasp the present invention by embodiment.But provided case is provided for protection of the present invention and claim scope.
Embodiment 1: the clone of gene
Gliocladium roseum (Gliocladium roseum) complete genome DNA of take carries out pcr amplification as template, and wherein PCR process primer used is respectively:
Forward primer:
5′-G
TTAATTAAAGGATGCCTGAGCACACACTCTCCCCCC-3′；
Reverse primer:
5′-TGC
TCTAGACTAGTGTACGTTGTTCTGCTTGCGG-3′；
Wherein underscore place represents respectively the restriction enzyme site of Pac1, two restriction enzymes of Xbal.
Pcr amplification condition is: 98 ℃ of 30s; 98 ℃ of 10s; 72 ℃ 1.5min30 circulation; 72 ℃ of 10min, archaeal dna polymerase used is Phusion archaeal dna polymerase; Utilize gel to reclaim test kit and reclaim pcr amplification product.
With Pac1 and two kinds of restriction enzymes of Xba1, respectively PCR product and carrier pGm being carried out to enzyme cuts; Use glue purification test kit to cut product to these two kinds of enzymes and carry out purifying, and use T
4dNA ligase connects above-mentioned two enzymes and cuts product; Product after connecting is transformed to bacillus coli DH 5 alpha, with penbritin, select.Correct for guaranteeing, several transformants are carried out bacterium colony PCR and checked order.
Sequencing result demonstration, the nucleotides sequence of above-mentioned pcr amplification product is classified SEQ ID NO:2 as; The aminoacid sequence of its coding is SEQ ID NO:1; A plurality of clones' sequencing result is all consistent.
Through NCBI Blast comparison, find, SEQ ID NO:1 is the highest with the catalase homology that derives from Pseudogymnoascus destructans, is 64%, with the catalase homology that derives from Beauveria bassiana be 63%.Therefore show that the present invention should be a catalatic neomorph of coding.
Use in plasmid amount to prepare test kit (Axygen) and from the correct escherichia coli cloning of sequencing result, extract recombinant plasmid, called after pGm-2092 (plasmid map is shown in Fig. 1).
Embodiment 2 recombinant plasmid transformed aspergillus niger G1 bacterial strain and checkings
Draw aspergillus niger (Aspergillus niger) G1 spore suspension in the dull and stereotyped center (9cm culture dish) of CMA, treat that bacterium colony covers with whole culture dish, cut the cultivation of 1/4 size based in 200mL CMA liquid nutrient medium, at 30 ℃, the condition of 200rpm, cultivate 14～16h.
With aseptic Miracloth filter cloth, collect thalline, and rinse once with solution A, under aseptic condition, with cotton swab, transferred in the lysate of 40ml, under 30 ℃, 200rpm condition, cracking is two hours, with the formational situation of microscopic examination protoplastis.
With the aseptic above-mentioned lysate of Miracloth filter-cloth filtering, with two aseptic centrifuge tubes of 50ml, collect protoplastis and make the about 25ml of every pipe with solution B flushing constant volume.Centrifugal 5min supernatant discarded under 2000rpm condition, with 20ml solution B to precipitating again twice of washing and precipitating.Pellet resuspended, in 10mLsolution B, and is counted protoplastis with blood counting chamber.By protoplastis recentrifuge abandoning supernatant, according to blood counting chamber count results, add the resuspended precipitation of appropriate solution B, make protoplastis number 1 * 10
7individual/mL left and right.
On ice, in cooling in advance 15ml centrifuge tube, add the above-mentioned protoplastis of 100 μ L, 10 μ L recombinant plasmid pGm-2092,12.5 μ L solution C ice bath 20min.Meanwhile the upper strata test tube MMSA preparing in advance melted and be placed in 55 ℃ of water-baths.
Take out the 15ml centrifuge tube in ice bath, to it, add 1ml solution C, 2ml solution B and mix rear as mixed solution.
In each in the upper strata MMSA test tube of 3 thawings, add the above-mentioned mixed solution of 1ml to mix and fall in MMSA flat board immediately, this flat board is cultivated to 7-10d until grow transformant in 30 ℃ of incubators.
The transformant bacterial strain growing is linked in postsearch screening substratum and is cultivated until grow black bacterium colony; Extract the genomic dna of bacterium colony, and carry out pcr amplification according to PCR reaction conditions described in embodiment 1; By glue, reclaim and sequence verification, the correct bacterial strain of the result is positive transformant, wherein a strain positive transformant called after aspergillus niger ZL-3 (Aspergillus niger ZL-3).
Solution A:2.5mL1M K
2hPO
4, 2.5mL1M KH
2pO
4, 48.156g MgSO
4, add dlH
2o is to final volume 500mL, with the filtering with microporous membrane degerming of 0.22 μ m.
Solution B:5mL1M Tris (pH7.5), 2.77g CaCl
2, 109.32g sorbyl alcohol, adds dlH
2o is to final volume 500mL, with the filtering with microporous membrane degerming of 0.22 μ m.
Solution C:250g PEG4000,2.77g CaCl
2, 5mL1M Tris (pH7.5), adds dlH
2o is to final volume 500mL, with the filtering with microporous membrane degerming of 0.22 μ m.
Lysate: 0.6g lyase (Lysing Enzyme from Trichoderma harzianum, Sigma) is dissolved in 40mL solution A, with the filtering with microporous membrane degerming of 0.22 μ m.
MMSA is dull and stereotyped: 0.59g ethanamide (Sigma), 3.4g CsCl (Sigma), 0.52g KCl, 1.52g KH
2pO
4, 218.5g sorbyl alcohol, 1ml trace element (see below), 20g agar, adds dlH
2o, to final volume 972.5mL, adds 25mL40% glucose and 2.5mL20%MgSO with the filtering with microporous membrane degerming of 0.22 μ m after high pressure steam sterilization
4.
MMSA top-layer agar test tube: 0.59g ethanamide (Sigma), 3.4g CsCl (Sigma), 0.52g KCl, 1.52g KH
2pO
4, 218.5g sorbyl alcohol, 1ml trace element (see below), 10g low melting-point agarose, adds dlH
2o, to final volume 972.5mL, after high pressure steam sterilization, when substratum does not solidify, adds 25mL40% glucose and 2.5mL20%MgSO with the filtering with microporous membrane degerming of 0.22 μ m
4, be sub-packed in immediately afterwards in sterile test tube every pipe 10mL.
Trace element: at 250mL dlH
2in O, add 1g FeSO
47H
2o, 8.8g ZnSO
4.
7h
2o, 0.4g CuSO
45H
2o, 0.15g MnSO
44H
2o, 0.1g Na
2b
4o
710H
2o, 50mg (NH
4)
6mo
7o
244H
2o, the dense HCl of 0.2mL, uses dlH after dissolving completely
2o is settled to 1L, with the filtering with microporous membrane degerming of 0.22 μ m.
CMA is dull and stereotyped: 20g glucose, and 20g Fructus Hordei Germinatus extract, 1g peptone, 15g agar, adds dlH
2o is to final volume 1000mL, autoclaving.
CMA liquid nutrient medium: 20g glucose, 20g Fructus Hordei Germinatus extract, 1g peptone, adds dlH
2o is to final volume 1000mL, autoclaving.
Two sieve substratum: 0.59g ethanamide (Sigma), 3.4g CsCl (Sigma), 0.52g KCl, 1.52gKH
2pO
4, 1ml trace element, 20g agar, adds dlH
2o, to final volume 972.5mL, adds 25mL40% glucose and 2.5mL20%MgSO with the filtering with microporous membrane degerming of 0.22 μ m after high pressure steam sterilization
4.
The fermentation checking of embodiment 3 positive transformant bacterial strains
Picking positive transformant aspergillus niger ZL-3, is inoculated in 20ml TSB fermention medium, at 30 ℃, under the condition of 200rpm, cultivates 5d; 8 layers of filtered through gauze for gained fermented liquid, filtrate is centrifugal 10min under 14000g condition, collects supernatant liquor; On the SDS-PAGE glue that is 10% in concentration by supernatant liquor, carry out electrophoretic analysis.As shown in Figure 2, there is a protein band clearly at arrow indication 87.2kDa place to result, in the same size with the theoretical molecular of enzyme of the present invention, thereby illustrates that described enzyme obtains effective expression in aspergillus niger ZL-3.
TSB fermention medium: 12g NaNO
3, 0.5g KCl, 1.5g KH
2pO
4, 2.05g MgSO
47H
2o, 3.5g NaH
2pO
4h
2o, 45g Trypsin soybean broth, 70g Trisodium Citrate, 1g tween 80,1mL trace element (see below), adds dlH
2o, to final volume 700mL, adds 40% maltose of the filtering with microporous membrane degerming of 0.22 μ m for 300mL after autoclaving.
Trace element: at 250mL dlH
2in O, add 1g FeSO
47H
2o, 8.8g ZnSO
4.
7h
2o, 0.4g CuSO
45H
2o, 0.15g MnSO
44H
2o, 0.1g Na
2b
4o
710H
2o, 50mg (NH
4)
6mo
7o
244H
2o, the dense HCl of 0.2mL, uses dlH after dissolving completely
2o is settled to 1L, with the filtering with microporous membrane degerming of 0.22 μ m.
Embodiment 4 catalase activities are measured
1, measuring method: ultraviolet spectrophotometry
2, the principle of measuring: hydrogen peroxide has strong absorption under 240nm wavelength, catalase energy decomposition of hydrogen peroxide, makes the absorbancy (A of solution
240) reduce with the reaction times.According to the pace of change of measuring absorbancy, can measure catalatic activity.
3, mensuration process: by preheating 30min after ultraviolet spectrophotometer energising, return to zero with phosphate buffered saline buffer after absorbing wavelength 240nm place machine zero.With the transfer pipet of 5ml, accurately pipette 2.9ml substrate solution to 1cm quartz colorimetric utensil, with the micropipet of 50-250ul, pipette 100ul testing sample, add in above-mentioned quartz colorimetric utensil, after shaking up, at absorbing wavelength 240nm place, survey immediately A value, every an A value of 5s record, minute is that 30s. does linear function curve by absorbancy to the time in Excel, and the absolute value of choosing curvilinear equation slope K calculates enzyme and lives.Control K value between 0.0031-0.0020, if K not in this region, need be diluted to sample suitable multiple again, measure.
4, enzyme activity calculates:
Enzyme activity (IU/ml or IU/g)=N * K * 4.13 * 10
4
In formula:
N-extension rate,
The absolute value of the slope of a curve that K-absorbancy changes,
The enzyme work that employing aforesaid method records recombinant bacterial strain aspergillus niger ZL-3 fermented supernatant fluid, up to 640.15U/mL, has also illustrated that enzyme of the present invention has catalase activity.
Embodiment 5 zymologic property analyses
1, optimum temperature
With NaH
2pO
4-Na
2hPO
4for buffered soln (pH7.0), under 30 ℃, 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ conditions, measure respectively the enzyme of above-mentioned aspergillus niger ZL-3 fermented liquid supernatant and live, make temperature-enzyme curve (Fig. 3) alive.As can be seen from Figure 3, the optimum temperature of enzyme of the present invention is 60 ℃.
2, the suitableeest action pH
Sodium phosphate dibasic-citrate buffer solution that the pH of take is 3.0,4.0,5.0,6.0,7.0,8.0 dilutes respectively above-mentioned aspergillus niger ZL-3 fermented liquid supernatant, and under 60 ℃ of conditions, measures the enzyme work of supernatant, makes pH-enzyme curve (Fig. 4) alive.As can be seen from Figure 4, the suitableeest action pH of enzyme of the present invention is 6.0.
The practical application of embodiment 6 enzymes of the present invention
1, reagent:
1) use NaH
2pO
4-Na
2hPO
4for buffered soln (pH7.0) compound concentration is 200mg/L H
2o
21L;
3) enzyme 1000U/mL of the present invention.
2, instrument:
The glassware such as volumetric flask; BL-310A precise electronic balance; DragonMed standard pipettor 100～1000ul; 5～50ul; The digital quartz electronic stopwatch of DMI-010 diamond plate.
3, measuring method:
1) measuring principle:
Hydrogen peroxide endonuclease capable decomposing H
2o
2generate oxygen G&W, by enzyme and H
2o
2after effect for some time, use H
2o
2test paper is tested remaining H
2o
2amount, the catalatic effect of the less explanation of residual volume is better.
2) specifically measure:
Measure 100mL H
2o
2solution (200mg/L), 40 ℃ of water-baths, preheating 5min; Then add 0.5mL enzyme of the present invention (1000U/mL), timing immediately (starting the clock during liquid feeding); Add after enzyme liquid, stir, obtain liquid to be measured; During respectively at 5min, 10min, 15min, 20min, by H
2o
2test paper (Merck) is dipped in liquid to be measured, takes out reading after 15sec after 1sec.As a control group with commercially available hydrogen peroxide enzyme product, be diluted to 1000U/mL simultaneously, the in the situation that of identical addition, carry out effect comparison with enzyme of the present invention.Concrete outcome is as shown in table 1:
Table 1 catalase is to H
2o
2the impact of concentration
From the results shown in Table 1, utilize enzyme of the present invention to process H
2o
2, during 15min, be about to H
2o
2all decompose; And commercially available catalase described in control group, when 20min, ability is by H
2o
2all decompose, and it is at 5min, 10min, during 15min to H
2o
2decomposition effect all lower than enzyme of the present invention.Thereby effectively decomposing H of enzyme of the present invention is described
2o
2, and effect is better than commercially available prod, and application prospect is extensive.
Enzyme of the present invention has catalase activity, effectively catalyzing hydrogen peroxide decomposes generation water and oxygen, therefore can be widely used in the fields such as food, weaving, medicine, papermaking and removes the hydrogen peroxide in production process, more efficient than traditional method, energy-conservation, environmental protection.
Claims (8)
1. a gene, is characterized in that, the enzyme that described genes encoding is following:
1) aminoacid sequence is the enzyme of SEQ ID NO:1,
2) 1) in amino acid on replace, lack or add that one or several amino acid obtains, have 1) in the activity of enzyme, by 1) derivative enzyme.
2. gene as claimed in claim 1, is characterized in that, the nucleotides sequence of described gene is classified SEQ ID NO:2 as.
3. a recombinant expression vector, is characterized in that, described recombinant expression vector carries gene claimed in claim 1.
4. a recombinant host cell, is characterized in that, described recombinant host cell carries recombinant expression vector described in claim 3.
5. recombinant host cell as claimed in claim 4, is characterized in that, described host cell is aspergillus niger.
6. the application of recombinant host cell claimed in claim 4 in degraded hydrogen peroxide.
7. an enzyme, is characterized in that, described enzyme is by genes encoding claimed in claim 1, and its aminoacid sequence is SEQ ID NO:1.
8. the application of enzyme claimed in claim 7 in degraded hydrogen peroxide.
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Cited By (4)
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|---|---|---|---|---|
| CN107384886A (en) * | 2017-08-28 | 2017-11-24 | 王艺璇 | A kind of new catalase and its application |
| CN107384814A (en) * | 2017-08-28 | 2017-11-24 | 王艺璇 | A kind of bacterial strain for recombinantly expressing catalase and its application |
| CN110540974A (en) * | 2019-09-29 | 2019-12-06 | 上海市农业科学院 | Volvariella volvacea (Volvariella volvacea) catalase VCAT as well as coding gene and application thereof |
| CN112522227A (en) * | 2020-12-21 | 2021-03-19 | 广东溢多利生物科技股份有限公司 | Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107384886A (en) * | 2017-08-28 | 2017-11-24 | 王艺璇 | A kind of new catalase and its application |
| CN107384814A (en) * | 2017-08-28 | 2017-11-24 | 王艺璇 | A kind of bacterial strain for recombinantly expressing catalase and its application |
| CN107384814B (en) * | 2017-08-28 | 2020-03-31 | 王艺璇 | Bacterial strain for recombinant expression of catalase and application thereof |
| CN110540974A (en) * | 2019-09-29 | 2019-12-06 | 上海市农业科学院 | Volvariella volvacea (Volvariella volvacea) catalase VCAT as well as coding gene and application thereof |
| CN112522227A (en) * | 2020-12-21 | 2021-03-19 | 广东溢多利生物科技股份有限公司 | Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application |
| CN112522227B (en) * | 2020-12-21 | 2022-09-23 | 广东溢多利生物科技股份有限公司 | Catalase with high enzyme activity, gene, recombinant strain with high catalase yield and application |
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| Publication number | Publication date |
|---|---|
| CN104212820B (en) | 2016-09-21 |
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