CN101245331A - South pole marine microorganism bacterial strain n2a of high-production low-temperature catalase - Google Patents
South pole marine microorganism bacterial strain n2a of high-production low-temperature catalase Download PDFInfo
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- CN101245331A CN101245331A CNA2008100579697A CN200810057969A CN101245331A CN 101245331 A CN101245331 A CN 101245331A CN A2008100579697 A CNA2008100579697 A CN A2008100579697A CN 200810057969 A CN200810057969 A CN 200810057969A CN 101245331 A CN101245331 A CN 101245331A
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Abstract
The invention relates to a high-yield and low-temperature antarctic marine microbial strain n2a of catalase which is separated and screened from the antarctic seawater, which is a new bacterial strain of Bacillus, the bacterial strain is collected in CCTCC, the collection number is CCTCC No: M207079, the activity of the catalase can achieve 1200U/mg, so the bacterial strain has wide application in the fields like industry, medicine, etc.
Description
Technical field
The present invention relates to the marine microorganism field, particularly relate to the low temperature catalase that from the seawater of the South Pole, separates the catalatic South Pole of high-yield of low-temperature ocean microorganism strains n2a and generation thereof.
Background technology
Marine site, the South Pole is because weather is cold especially, and environment is extremely abominable, so the animals and plants kind is less, but a large amount of prokaryotic organism is arranged, the psychrophile aboundresources.Cold-adapted enzyme is a key of keeping the biochemical reaction rate that cell activities is suitable under the cold condition, and screening cold-adapted enzyme superior strain is the basis of psychrophile theory and application research.
Catalase is the important albumen of running one's home in the aerobic microbiological vital movement.It removes the strong oxidizer H that produces rapidly in the redox reaction of bio-metabolic process
2O
2, prevent that it from passing cell inner structure, oxidized protein or fracture nucleic acid strand injury cell.It shows wide application prospect with the ability of efficient catalytic in fields such as industry and medical treatment again in recent years.So the low temperature catalase is significant to aerobic psychrophile vital movement as the biological antioxidant protective material, also be the cold-adapted enzyme that potential industrial application value is arranged.
Hernandez reported once that Antarctic Ocean water bacteria was at 50 μ mol/LH
2O
2Condition under still can normal growth, the bacterial classification that has high catalase activity in might South Pole seawater is described.
People such as people such as Yumoto and Lorentzen have reported the high reactivity low temperature catalase from Vibrio rumoiensis and Vibriosalmonicida respectively, but the catalatic microorganism of high reactivity low temperature from the natural surroundings free living does not still have report, and the ocean gram-positive microorganism of high catalase activity does not have report yet.
Summary of the invention
The objective of the invention is to seek existence catalatic microorganism of high yield high reactivity low temperature under the extreme low temperature condition particularly in physical environment; through contriver's development research extensively and profoundly; psychrophile carries out on the separation screening basis in to South Pole seawater, obtains ocean, the catalatic South Pole of the protectant high yield high reactivity of the biological antioxidant low temperature microorganism strains Bacillus sp.n2a (being called for short bacterial strain n2a) that a strain has the efficient catalytic ability.
Ocean, the catalatic South Pole of high-yield of low-temperature provided by the invention microorganism strains Bacillus sp.n2a is ocean, the catalatic South Pole of the high yield high reactivity low temperature microorganism strains Bacillus sp.n2a that separation screening obtains from the seawater of the South Pole, is respectively according to the 16SrDNA phylogenetic tree (Fig. 1) of ocean, South Pole microorganism strains Bacillus sp.n2a and relevant bacterial strain and the highest bacterial strain of 16SrDNA sequence similarity of bacterial strain n2a:
Bacillus barbaricus DSM 14730
T(98.8％)，
Bacillus arsenicus DSM 15822
T(97.5％)，
Bacillus macauensis DSM 17262
T(96.4％)，
Bacillus gelatini LMG 21880
T(95.1％)。
Identifying the bacterium that is defined as bacillus through 16SrDNA gene sequencing (GenBanK accession number DQ508485), is the novel bacterial of Bacillus.This ocean, high-yield of low-temperature catalase South Pole microorganism strains Bacillus sp.n2a is deposited in Chinese typical culture collection center, preserving number: CCTCCNo:M207079 on June 9th, 2007.
Form and the physiological and biochemical property of ocean, the catalatic South Pole of high-yield of low-temperature provided by the invention microorganism strains Bacillus sp.n2a are:
Morphological specificity:
Bacterial strain n2a is a genus bacillus, long 2.5-5 μ m, and wide 0.5-1 μ m, oval gemma end is given birth to.
Cultural characteristic:
(described basic sea water medium is that 5 gram peptones and 1 gram yeast extract paste are dissolved in 1 liter of seawater in basic sea water medium, pH7.5, sterilized 20 minutes for 121 ℃) under 4 ℃～40 ℃ temperature, grow, its optimum growth temperature scope is 20 ℃～30 ℃, growth pH scope is pH6～11, the NaCl concentration of suitable growth is 0%～6%, when the growth greater than 8% time of NaCl concentration is suppressed.30 ℃ of basic sea water mediums are cultivated the bacterium colony circle of 24h, full edge, flat, and diameter 0.9 is to 1.2mm, and is smooth glossy, and mucus shape outward appearance, quality are like cream, and white is translucent.
Physiological and biochemical property
The physiological and biochemical property tabulation 1 of bacterial strain n2a.
Table 1
| Feature | |
| Oxydase | - |
| Methyl red | - |
| V-P | - |
| Indoles | - |
| H 2S produces | - |
| Cellulase | - |
| Tween20 hydrolysis (polyethylene oxide Span 20) | - |
| The Tween80 hydrolysis | - |
| (polyethylene oxide dehydrating sorbitol monooleate) | - |
| The nitrite reduction | - |
| Oxidation of ethanol | - |
| The L-phenylalanine deaminase | - |
| Arginine dihydrolase | - |
| The hydrolysis urobenzoic acid | - |
| Utilize tartrate | - |
| Utilize sugar or pure aerogenesis | - |
| The RNA enzyme | + |
| Alkaline phosphatase | + |
| Gelatine liquefication | + |
| Casein decomposes | + |
| Utilize glucose | Produce acid |
| Starch | Produce acid |
| Glycerine | Produce acid |
| Maltose | Produce acid |
| Sucrose | Produce acid |
| Trehalose | Produce acid |
| Seminose | Produce acid |
| Wood sugar | Do not produce acid |
| Lactose | Do not produce acid |
| N.F,USP MANNITOL | Do not produce acid |
| Sorbyl alcohol | Do not produce acid |
| Ethylene glycol | Do not produce acid |
| Methyl alcohol | Do not produce acid |
Bacterial strain n2a has the bacillus form and produces the key character of these two kinds of bacillus of gemma ability.
The bacterial strain phenotypic characteristic
Bacterial strain n2a lists in table 2 with the difference of the phenotypic characteristic of the relevant bacterial classification of phylogeny
Table 2
| Feature | 1 | 2 | 3 | 4 | 5 |
| Gramstaining long-chain morphology logy 4 ℃ of 47 ℃ of pH, the 9.5 5%NaCl anaerobic growth reactivity nitrate reductions of growing | v - + - + + - + + | + - - - + - + - - | + - - - - - - + - | + + - - + - + ND ND | v + ND + + + - w - |
Annotate: 1, bacterial strain n2a; 2, Bacillus barbaricus; 3, Bacillus arsenicus; 4, Bacillusmacauensis; 5, Bacillus gelatini.+; Just: negative: ND: undetermined v: variable; W: a little less than.
Bacterial strain n2a obviously is different from the relevant bacterial strain of 16SrDNA phylogenetic systematics, 5 ℃ of growths of the phenotype of bacterial strain n2a, 7%NaCl growth at aspects such as low-temperature epitaxy, salt tolerances as seen from Table 2.
Bacterial strain n2a lists in table 3 with the fatty acid content (%) of the relevant bacterial classification of phylogeny.
Table 3
| Lipid acid | 1 | 2 | 3 | 4 | 5 |
| C13:0 iso-C13:0 iso-C14:0 C14:0 iso-C14:1 iso-C15:0 anti-one different-C15:0 C15:0 iso-C15:1 iso-C16:1 C16:1 ω 7cis C16:1 ω 11cis iso-C16:0 anti-one different-C16:0 C16:0 iso-C17:0 anti-one different-C17:0 anti-one different-C18:1 C18:1 ω 11cis | 0 4.7 13.3 0 17.6 14.2 14.2 0 2.4 11.2 6 1 2 1 0 0 0 2 5.7 | 0 0 9.4 0.9 0 19 42.8 0.9 0.7 0 4.9 2.8 6 0 2 0 2.6 0 0 | 5.8 0 3.4 1.1 0 10.6 10.5 1.6 4.2 0 25.3 4.2 19 0 9.6 1.1 0 0 0 | 0 0 4.6 2.2 0 21.7 67.2 0 0 0 0 0 1.6 0 0 2.7 0 0 0 | 0 0 0 0 0 60.7 9 0 0 0 0 0 4.6 0 2.2 12.8 9.7 0 0 |
Annotate: 1, n2a; 2, Bacillus barbaricus; 3, Bacillus arsenicus; 4, Bacillus macauensis; 5, Bacillus gelatini.
Branched chain fatty acid by the visible bacterial strain n2a of table 3 is preponderated, and promptly unsaturated fatty acids accounts for more than 40%, embodies the feature of low temperature ocean bacterium, and this meets the feature of bacillus fully.But the C14 of bacterial strain n2a, C15 and the main fatty acid content of C16 three classes are more or less the same (20% to 30%), and this obviously is different from the relevant bacterial strain (its C15 or C16 lipid acid is preponderated) of phylogenetic systematics.
The unsaturated fatty acid content of bacterial strain n2a surpasses 40%, and apparently higher than the relevant bacterial strain of 16SrDNA phylogenetic systematics, this also is the key character of psychrophile.
The cultivation of bacterial strain n2a
Primary dcreening operation: basic sea water medium sterilization postcooling to the 50 ℃ adding H that contains 1.5% (w/v) agar
2O
2, make final concentration reach 1mmol/L, fall dull and stereotyped.With 200 μ L seawater samples (near for example seawater Antarctic Zhongshan Station) coating, to cultivate 10 days for 15 ℃, the single bacterium colony that grows is drawn slant preservation.
Multiple sieve: the bacterial strain that primary dcreening operation obtains inserts 250mL and shakes bottle (containing 20mL basis sea water medium) from the inclined-plane, 200rpm cultivates 24h for 20 ℃, centrifugal receipts thalline, with pH7.5, resuspended thalline of 50mmol/LTris-Cl and ultrasonication in ice bath are got supernatant liquor after centrifugal and are surveyed activity and albumen.Catalase activity is with H
2O
2Separate rate calculations in 24Onm punishment.H
2O
2Molar absorptivity is 43.6 (mol/L)
-1Cm
-1, 1 unit of activity is defined as 5 ℃ of per minutes and decomposes 1 μ molH
2O
2The enzyme amount.Determining the protein quantity adopts the Bradford method.With M.lentus and Escherichia coli is contrast screening high reactivity bacterial strain.
The dull and stereotyped bacterial growth of primary dcreening operation is slow, occurs macroscopic bacterium colony after 7 days.All single bacterium colonies sieve the back again and obtain a plant height active bacterial strain n2a, and its thalline ultrasonication liquid catalase activity reaches 1200U/mg, and enzymic activity is respectively 900U/mg and 70U/mg in the thalline ultrasonication liquid of M.lentus and E.coli.
Catalase activity is relatively listed in table 4 in the broken liquid of bacterial strain n2a and other microbial cell
Table 4
| Microorganism | Catalase activity(U/mg ) |
| n2a Escherichia coli Micrococcus lentus Halomonas sp.SK1 Comamonas terrigena Rhodobacter sphaeroides Rhodospirillum rubrum Thermus brockianus Frankia strain R43 Desulfovibrio gigas Trigonopsis variabilis Neurospora crassa Halobacterium halobium Methanobrevibacter arboriphilus | 1200 70 900 360 290 184 88 82 74 52.6 188 25.6 60.6 240 |
The high 1200U/mg that reaches of catalase activity by the visible bacterial strain n2a of table 4.Can obtain the high reactivity bacterial strain with the dull and stereotyped primary dcreening operation that contains high-strength hydrogen peroxide.
Provided by the invention is small subunit list function catalase (BNC) from Antarctic Ocean water sepn acquisition high-yield of low-temperature catalase, is that molecular weight is the homotetramer of 230kD, molecular weight subunit 56kD, pI=4.2.25 ℃ of its optimum temperutures, optimal pH 6-11, activity is subjected to the inhibition of sodium azide, oxammonium hydrochloride and mercaptoethanol.The activation energy 13kJ of BNC is respectively 3.6 * 10 at the catalytic efficiency of 4 ℃ and 25 ℃
6With 4 * 10
6M
-1s
-1High catalytic efficiency guarantees that the hydrogen peroxide that metabolism produces is removed rapidly under the BNC low temperature.As the catalatic representative of low temperature small subunit list function, in BNC and the homology between the activation energy of warm enzyme, catalytic efficiency and the thermostability difference little, become the special case in the cold-adapted enzyme.To be used widely in fields such as industry and medical treatment.
Description of drawings:
Fig. 1 is the 16SrDNA phylogenetic tree of bacterial strain n2a and relevant bacterial strain
Embodiment
The present invention further specifies the present invention with the following example, but protection scope of the present invention is not limited to the following example.
Embodiment 1
Primary dcreening operation: basic sea water medium (5 gram peptones and 1 gram yeast extract paste are dissolved in the 1L seawater, transfer PH7.5,121 ℃ sterilization 20 minutes) the sterilization postcooling to 50 that contains 1.5% (w/v) agar ℃ adds H
2O
2, make final concentration reach 1mmol/L, fall dull and stereotyped.With 200 μ L seawater samples (near the seawater sample Antarctic Zhongshan Station) coating, cultivated 10 days for 15 ℃.The single bacterium colony that grows is drawn slant preservation.
Multiple sieve: the bacterial strain that primary dcreening operation obtains inserts 250mL and shakes bottle (containing 20mL basis sea water medium) from the inclined-plane, 200rpm cultivates 24h for 20 ℃.Centrifugal receipts thalline, with pH7.5,50mmol/LT ris-Cl (get supernatant liquor after centrifugal and survey activity and albumen by Tutofusin tris-Cl) resuspended thalline and ultrasonication in ice bath.Record catalase activity and reach 1200 U/mg, get bacterial strain n2a.
Bacterial strain n2a is a genus bacillus, long 2.5-5 μ m, and wide 0.5-1 μ m, oval gemma end is given birth to.Grow under 4 ℃～40 ℃ temperature in the sea water medium of basis, its optimum growth temperature scope is 20 ℃～30 ℃, and its growth pH scope is pH6～11.The NaCl concentration of suitable growth is 0%～6%, when the growth greater than 8% time of NaCl concentration is suppressed.30 ℃ of basic sea water mediums are cultivated the bacterium colony circle of 24h, full edge, flat, and diameter 0.9 is to 1.2mm, and is smooth glossy, and mucus shape outward appearance, quality are like cream, and white is translucent; Oxydase, methyl red, V-P, indoles, H
2S generation, cellulase, Tween20 hydrolysis, Tween80 hydrolysis, nitrite reduction, oxidation of ethanol, L-phenylalanine deaminase, arginine dihydrolase, hydrolysis urobenzoic acid, utilize tartrate, utilize sugar or the experiment of pure aerogenesis all to belong to negative.RNA enzyme, alkaline phosphatase, gelatine liquefication, casein decompose, utilize glucose, starch, glycerine, maltose, sucrose, trehalose, seminose to produce the acid experiment, and all genus is positive; Can not produce acid from wood sugar, lactose, N.F,USP MANNITOL, sorbyl alcohol, ethylene glycol, methyl alcohol.
The phenotypic characteristic of bacterial strain n2a
Gramstaining v
Long-chain morphology-
Growth
4℃ +
47℃ -
pH 9.5 +
5％NaCl +
Anaerobic growth-
Reactivity+
Nitrate reduction+
Bacterial strain n2a fatty acid content
C13:0 0
iso-C13:0 4.7
iso-C14:0 13.3
C14:0 0
iso-C14:1 17.6
iso-C15:0 14.2
anteiso-C15:0 14.2
C15:0 0
iso-C15:1 2.4
iso-C16:1 11.2
C16:1ω7cis 6
C16:1ω11cis 1
iso-C16:0 2
Anti-one different-C16:0 1
C16:0 0
iso-C17:0 0
Anti-one different-C17:0 0
Anti-one different-C18:1 2
C18:1ω11cis 5.7
Sequence table
＜110〉Inst of Huanghai Sea Marine Products, Chinese Academy of Aquatic Product Science
＜120〉ocean, the catalatic South Pole of high-yield of low-temperature microorganism strains n2a
<160>1
<210>1
<211>1434
<212>DNA
＜213〉ocean, South Pole microorganism strains n2a (Bacillus sp.n2a)
<400>1
gcctaataca tgcaagtcga gcgaatgatg aggagcttgc tcctctgatt tagcggcgga 60
cgggtgagta acacgtgggt aatctgcctg taagacgggg ataactccgg gaaaccgggg 120
ctaataccgg ataacaagag aagaagcatt tcttcttttt gaaagtcggt ttcggctgac 180
acttacagat gagcccgcgg cgcattagct agttggtgag gtaacggctc accaaggcga 240
cgatgcgtag ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac 300
tcctacggga ggcagcagta gggaatcttc ggcaatgggc gaaagcctga ccgagcaacg 360
ccgcgtgagc gatgaaggcc ttcgggtcgt aaagctctgt tgttagagaa gaacaagtac 420
gagagtaact gctcgtacct tgacggtacc taaccagaaa gccacggcta actacgtgcc 480
agcagccgcg gtaatacgta ggtggcaagc gttatccgga attattgggc gtaaagcgcg 540
cgcaggcggt ctcttaagtc tgatgtgaaa gcccacggct caaccgtgga gggtcattgg 600
aaactgggag acttgagtgc aggagagaaa agtggaattc cacgtgtagc ggtgaaatgc 660
gtagagatgt ggaggaacac cagtggcgaa ggcggctttt tggcctgtaa ctgacgctga 720
ggcgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga 780
tgagtgctag gtgttggggg gttccaccct cagtgctgaa gttaacacat taagcactcc 840
gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc 900
agtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct 960
ctgaccactc tagagataga gctttcccct tcgggggaca gagtgacagg tggtgcatgg 1020
ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttga 1080
ccttagttgc cagcattcag ttgggcactc taaggtgact gccggtgaca aaccggagga 1140
aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa 1200
tggatggtac aaagggttgc gaagccgcga ggccaagcca atcccaaaaa gccattctca 1260
gttcggattg taggctgcaa ctcgcctaca tgaagccgga attgctagta atcgcggatc 1320
agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag 1380
tttgtaacac ccgaagtcgg tggggtaacc cttttgggag ccagccgccg aagg 1434
Claims (4)
1, ocean, the catalatic South Pole of a kind of high-yield of low-temperature microorganism strains n2a, it is characterized in that it being from the seawater of the South Pole, to separate ocean, the catalatic South Pole of the high-yield of low-temperature microorganism strains Bacillus sp.n2a that obtains, it is the novel bacterial of the Bacillus of bacillus, this bacterial strain is deposited in Chinese typical culture collection center, and preserving number is CCTCCNo:M207079.
2,, it is characterized in that ocean, the catalatic South Pole of described high-yield of low-temperature microorganism strains Bacillus sp.n2a form and physiological and biochemical property are according to the catalatic South Pole of a kind of high-yield of low-temperature ocean microorganism strains n2a of claim 1:
Morphological specificity:
Bacterial strain n2a is a genus bacillus, long 2.5-5 μ m, and wide 0.5-1 μ m, oval gemma end is given birth to;
Cultural characteristic:
Growing under 4 ℃～40 ℃ temperature in basic sea water medium, its optimum growth temperature scope are 20 ℃～30 ℃, and growth pH scope is pH6～11, and the NaCl concentration of suitable growth is 0%～6%, and NaCl concentration is suppressed greater than growth in 8% o'clock; 30 ℃ of basic sea water mediums are cultivated the bacterium colony circle of 24h, full edge, flat, and diameter 0.9 is to 1.2mm, and is smooth glossy, and mucus shape outward appearance, quality are like cream, and white is translucent;
Physiological and biochemical property:
The physiological and biochemical property of bacterial strain n2a is
3, according to the catalatic South Pole of the high-yield of low-temperature of claim 1 ocean microorganism strains n2a, it is characterized in that this bacterial strain n2a 16srRNA complete sequence is as follows, total length is 1434bp,
GCCTAATACATGCAAGTCGAGCGAATGATGAGGAGCTTGCTCCTCTGATTTAGCGGCGGACGGGTGAGTAACACG
TGGGTAATCTGCCTGTAAGACGGGGATAACTCCGGGAAACCGGGGCTAATACCGGATAACAAGAGAAGAAGCATT
TCTTCTTTTTGAAAGTCGGTTTCGGCTGACACTTACAGATGAGCCCGCGGCGCATTAGCTAGTTGGTGAGGTAAC
GGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGAC
TCCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGACCGAGCAACGCCGCGTGAGCGATGA
AGGCCTTCGGGTCGTAAAGCTCTGTTGTTAGAGAAGAACAAGTACGAGAGTAACTGCTCGTACCTTGACGGTACC
TAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT
TGGGCGTAAAGCGCGCGCAGGCGGTCTCTTAAGTCTGATGTGAAAGCCCACGGCTCAACCGTGGAGGGTCATTGG
AAACTGGGAGACTTGAGTGCAGGAGAGAAAAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGG
AACACCAGTGGCGAAGGCGGCTTTTTGGCCTGTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATT
AGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAGGTGTTGGGGGGTTCCACCCTCAGTGCTGAAGTTAA
CACATTAAGCACTCCGCCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGC
AGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACCACTCTAGAG
ATAGAGCTTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGG
GTTAAGTCCCGCAACGAGCGCAACCCTTGACCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGG
TGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAA
TGGATGGTACAAAGGGTTGCGAAGCCGCGAGGCCAAGCCAATCCCAAAAAGCCATTCTCAGTTCGGATTGTAGGC
TGCAACTCGCCTACATGAAGCCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCC
TTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGGGGTAACCCTTTTGGGAGCCAGC
CGCCGAAGG。
4, the low temperature catalase that produces according to the catalatic South Pole of the high-yield of low-temperature ocean microorganism strains n2a of claim 1 or 2 or 3.
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| CN102864106A (en) * | 2012-09-24 | 2013-01-09 | 国家海洋局第二海洋研究所 | Ocean catalase production bacteria and directional screening method |
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| CN102864106A (en) * | 2012-09-24 | 2013-01-09 | 国家海洋局第二海洋研究所 | Ocean catalase production bacteria and directional screening method |
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| CN103060244A (en) * | 2013-01-21 | 2013-04-24 | 国家海洋局第二海洋研究所 | Bacillus marinus and method for producing catalase by using same |
| CN104212820A (en) * | 2014-09-15 | 2014-12-17 | 青岛蔚蓝生物集团有限公司 | Enzyme with catalase activity and encoding gene of enzyme |
| CN104212820B (en) * | 2014-09-15 | 2016-09-21 | 青岛蔚蓝生物集团有限公司 | A kind of enzyme with catalase activity and encoding gene thereof |
| CN106566817A (en) * | 2016-10-28 | 2017-04-19 | 中国水产科学研究院黄海水产研究所 | Cloning and expression of gene of catalase produced by marine microbial strain YS0810 |
| CN111440807A (en) * | 2020-04-07 | 2020-07-24 | 上海海洋大学 | Shewanella WP3 mutant strain with high yield of low-temperature catalase as well as construction method and application thereof |
| CN112143721A (en) * | 2020-11-26 | 2020-12-29 | 中国农业科学院生物技术研究所 | Low-temperature catalase and preparation method and application thereof |
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