CN104207054A - Method for extracting amino acids from Canna edulis - Google Patents
Method for extracting amino acids from Canna edulis Download PDFInfo
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- CN104207054A CN104207054A CN201410310667.1A CN201410310667A CN104207054A CN 104207054 A CN104207054 A CN 104207054A CN 201410310667 A CN201410310667 A CN 201410310667A CN 104207054 A CN104207054 A CN 104207054A
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- lotus root
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- irrigated lotus
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 24
- 244000292211 Canna coccinea Species 0.000 title abstract description 7
- 235000005273 Canna coccinea Nutrition 0.000 title abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 53
- 238000002791 soaking Methods 0.000 claims abstract description 27
- 239000012141 concentrate Substances 0.000 claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 238000002386 leaching Methods 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 240000002853 Nelumbo nucifera Species 0.000 claims description 41
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 41
- 235000012054 meals Nutrition 0.000 claims description 15
- 238000010992 reflux Methods 0.000 claims description 15
- 239000000284 extract Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical group C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012670 alkaline solution Substances 0.000 claims description 3
- 238000009835 boiling Methods 0.000 claims description 3
- 239000003729 cation exchange resin Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000003797 essential amino acid Substances 0.000 abstract description 10
- 235000020776 essential amino acid Nutrition 0.000 abstract description 10
- 229920002472 Starch Polymers 0.000 abstract description 6
- 235000019698 starch Nutrition 0.000 abstract description 5
- 239000008107 starch Substances 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 4
- 239000000243 solution Substances 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000000843 powder Substances 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 2
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 description 15
- 230000009286 beneficial effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 229920000945 Amylopectin Polymers 0.000 description 1
- 235000019890 Amylum Nutrition 0.000 description 1
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 244000205754 Colocasia esculenta Species 0.000 description 1
- 235000006481 Colocasia esculenta Nutrition 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 1
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
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- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 244000273928 Zingiber officinale Species 0.000 description 1
- 235000006886 Zingiber officinale Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 1
- 229960002646 scopolamine Drugs 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention relates to the field of deep processing of crops, and especially relates to a method for extracting amino acids from Canna edulis. The method comprises the following steps: 1, crushing, and soaking; 2, filtering, and concentrating; 3, carrying out alcohol leaching; 4, extracting; and 5, carrying out exchange elution. The method is characterized in that Canna edulis is ground to form powder, the powder is soaked to make starch and other substances soaked out, secondary treatment benefits the filter out of impurities in a Canna edulis soaking liquid, nutrition substances in the Canna edulis are well concentrated in a concentrate under a certain pressure, the concentrate undergoes cold leaching and alcohol dilution to make essential amino acid components in the concentrate diluted and extracted in an ethanol solution. The method has the advantages of accurate steps, extraction of the amino acid components from the Canna edulis, full extraction and very broad development prospect.
Description
Technical field
The present invention relates to crops deep processing field, relate in particular to the amino acid whose extracting method of a kind of non-irrigated lotus root.
Background technology
Drought lotus root is Araeceae perennial herb legume, and raw cultivation in 1 year, has spherical-shaped piece root.The rare special product in Dabie Mountain is a kind of thick life, without the amylum crops of disease and pest, its stem tuber is rich in amylopectin.It is not only the rare famous and precious vegetables that integrate vegetables, herbal cuisine, grain, or rare gardening ornamental plant.Be grown in Subtropical Mountainous.The fertile survival dose of drought lotus root is few, is the famous and precious vegetables of high-quality, low input, nuisanceless, high nutrition, high benefit.
Secondly non-irrigated lotus root also can be medicinal, has another name called the prince's descendants, male illiteracy, edible canna.Rhizome hyoscine, has the effects such as tonifying Qi, dispellieg cold and dampness.The new Tang of < < book side's skill passes ginger and comforts > >: " there is non-irrigated lotus root in the Zhonnanshan Mountain." consult bright Li Shizhen (1518-1593 A.D.) < < Compendium of Materia Medica grass one prince's descendants > >.Its underground root contains abundant nourishing starch, crude fibre, crude protein, reduced sugar, various trace elements calcium, phosphorus, iron and 17 seed amino acids, Cobastab, C, not only nutritious but also be easy to digest, often edible beneficial blood is mended marrow, and the hot moistening lung that disappears, prevents obesity, enriching yin and nourishing kidney.At pot for cooking rice steamed wheaten foods or section, burn stewed duck soup, or beat powder or process fragrant taro sheet edible, crisp glutinous smooth, the human world is delicious, especially selenium element is beneficial to anti-senile angiocardiopathy, osteoporosis, cataract, diabetes, and have anti-colon cancer, help digest, the function such as clearing heat and detoxicating, strong stomach of invigorating the spleen, nourishing.
17 kinds of essential amino acids that contain needed by human body in drought lotus root, although essential amino acid aequum is little, the damage that lacks human body can not be ignored.Essential amino acid can decompose and compound body internal protein, plays nitrogen balance effect; Can change sugar or fat into, energy is provided; Participate in forming enzyme, hormone, part vitamin, in regulating physiological function, catalysis metabolic process, play a very important role.In patent, periodical, have never seen and from non-irrigated lotus root, extract amino acid whose relevant report, and very meticulous for extract amino acid whose method step from non-irrigated lotus root, the result of slightly being forbidden to draw can not extract exactly amino acid whose amount or extracted amount few.
Summary of the invention
The invention provides and a kind ofly can from non-irrigated lotus root, extract the extracting method of essential amino acid, it is characterized in that, by following steps, realize:
1) pulverize and soak: non-irrigated lotus root is cleaned up, be polished into meal and with soaking 12-16 hour under clear water normal temperature, obtain non-irrigated lotus root meal soak, non-irrigated lotus root grinds is also soaked to be beneficial to the materials such as starch are soaked out, be convenient to the extraction of back step;
2) the non-irrigated lotus root meal soak obtaining filtering and concentrating: by step 1) carries out secondary filter processing, getting filtered fluid is placed in enrichment facility and carries out reduced pressure concentration, carry out that after-treatment is useful gives the impurity in non-irrigated lotus root soak to filter out, pressure is controlled and within the specific limits the nutriment in non-irrigated lotus root is concentrated in concentrate well;
3) it is that the ethanol of 78%-80% carries out stir process that the concentrate alcohol leaching: in step 2) adds mass percent concentration, then carrying out a cold soaking processes, the cold soaking time is 40-60 minute, then getting supernatant, to add mass percent be that the ethanol of 80%-85% carries out the processing of secondary cold soaking again, the cold soaking time is 40-60 minute, and cold soaking alcohol is rare to be diluted in ethanolic solution in order to allow essential amino acid composition in concentrate complete;
4) the alcohol immersion liquid extraction: by step 3) is placed in reflux and first carries out alcohol reflux processing, and then carries out boiling water refluxing extraction, the advanced row alcohol extracting of alcohol immersion liquid is carried out to water extraction again and get, and step is accurate, extracts fully;
5) exchange wash-out: by step 4) in reflux extracting liquid be placed in storng-acid cation exchange resin exchange processing then with alkaline solution, carry out wash-out, the essential amino acid of the liquid of collecting for extracting from non-irrigated lotus root, under acid condition, on positively charged amino and branch-SO
3h exchange, can exchange amino acid.
Preferably, the described amino acid whose extracting method of non-irrigated lotus root, is characterized in that step 1) in the particle diameter of meal be 30-50 order, the solid-liquid ratio of meal and clear water is 1: 10-1: 15.
Preferably, the described amino acid whose extracting method of non-irrigated lotus root, is characterized in that step 2) in concentrated pressure be controlled at 50-60kpa.
Preferably, the described amino acid whose extracting method of non-irrigated lotus root, it is characterized in that, step 3) in, in a cold soaking processing, concentrate is 1 with adding the ratio of ethanol: 10-1: 20, in the processing of secondary cold soaking, concentrate is 1 with adding the ratio of ethanol: 8-1: 15, and the temperature of cold soaking is controlled at 15-20 ℃.
Preferably, the described amino acid whose extracting method of non-irrigated lotus root, is characterized in that step 4) in alcohol reflux extract in the concentration of ethanol be 78%-90%.
Beneficial effect of the present invention, by method step of the present invention, non-irrigated lotus root grinds is also soaked to be beneficial to the materials such as starch are soaked out, carry out that after-treatment is useful gives the impurity in non-irrigated lotus root soak to filter out, pressure is controlled and within the specific limits the nutriment in non-irrigated lotus root is concentrated in concentrate well, then through cold soaking alcohol is rare, in order to allow essential amino acid composition in concentrate complete to be diluted in ethanolic solution, extract, step is accurate, aminoacid ingredient in non-irrigated lotus root not only can be extracted, and extract fully, there is very vast potential for future development.
The specific embodiment
Following examples are to further illustrate of the present invention, but absolutely not limit the scope of the present invention.Below with reference to embodiment, further elaborate the present invention, but it will be appreciated by those skilled in the art that the present invention is not limited to the preparation method of these embodiment and use.And those skilled in the art can be equal to replacement, combination, improvement or modify the present invention according to description of the invention, these all comprise within the scope of the invention.
Embodiment 1
1) pulverize and soak: get 500 grams of non-irrigated lotus roots and clean up, be polished into meal, the particle diameter of meal is 30-50 order, add clear water, the solid-liquid ratio of meal and clear water is 1: 10, soaks 12 hours under normal temperature, obtains non-irrigated lotus root meal soak, non-irrigated lotus root grinds is also soaked to be beneficial to the materials such as starch are soaked out, be convenient to the extraction of back step;
2) the non-irrigated lotus root meal soak obtaining filtering and concentrating: by step 1) carries out secondary filter processing, getting filtered fluid is placed in enrichment facility and carries out reduced pressure concentration, pressure is controlled at 50kpa, carry out that after-treatment is useful gives the impurity in non-irrigated lotus root soak to filter out, pressure is controlled and within the specific limits the nutriment in non-irrigated lotus root is concentrated in concentrate well;
3) it is that 78% ethanol carries out stir process that the concentrate alcohol leaching: in step 2) adds mass percent concentration, in cold soaking processing, concentrate is 1: 10 with adding the ratio of ethanol, the temperature of cold soaking is controlled at 15 ℃, the cold soaking time is 60 minutes, then getting supernatant, to add mass percent be that 85% ethanol carries out the processing of secondary cold soaking again, in the processing of secondary cold soaking, concentrate is 1: 15 with adding the ratio of ethanol, the cold soaking time is 60 minutes, and cold soaking alcohol is rare to be diluted in ethanolic solution in order to allow essential amino acid composition in concentrate complete;
4) the alcohol immersion liquid extraction: by step 3) is placed in reflux and first carries out alcohol reflux processing, and then carrying out boiling water refluxing extraction, during alcohol reflux extracts, the concentration of ethanol is 78%, the advanced row alcohol extracting of alcohol immersion liquid is carried out to water extraction again and get, step is accurate, extracts fully;
5) exchange wash-out: by step 4) in reflux extracting liquid be placed in storng-acid cation exchange resin exchange processing then with alkaline solution, carry out wash-out, the essential amino acid of the liquid of collecting for extracting from non-irrigated lotus root, under acid condition, on positively charged amino and branch-SO
3h exchange, can exchange amino acid.
The amino acid extracting is free from foreign meter, and content is pure, and amino acid whose recovery rate is 95%.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment describing.
Claims (5)
1. the amino acid whose extracting method of non-irrigated lotus root, is characterized in that, comprises the following steps:
1) pulverize and soak: non-irrigated lotus root is cleaned up, be polished into meal and with soaking 12-16 hour under clear water normal temperature, obtain non-irrigated lotus root meal soak;
2) the non-irrigated lotus root meal soak obtaining filtering and concentrating: by step 1) carries out secondary filter processing, gets filtered fluid and is placed in enrichment facility and carries out reduced pressure concentration;
3) it is that the ethanol of 78%-80% carries out stir process that the concentrate alcohol leaching: in step 2) adds mass percent concentration, then carrying out a cold soaking processes, the cold soaking time is 40-60 minute, then getting supernatant, to add mass percent be that the ethanol of 80%-85% carries out the processing of secondary cold soaking again, and the cold soaking time is 40-60 minute;
4) the alcohol immersion liquid extraction: by step 3) is placed in reflux and first carries out alcohol reflux processing, and then carries out boiling water refluxing extraction;
5) exchange wash-out: by step 4) in reflux extracting liquid be placed in storng-acid cation exchange resin exchange processing then with alkaline solution, carry out wash-out, the liquid of collecting is the amino acid extracting in non-irrigated lotus root.
2. the amino acid whose extracting method of non-irrigated lotus root according to claim 1, is characterized in that step 1) in the particle diameter of meal be 30-50 order, the solid-liquid ratio of meal and clear water is 1: 10-1: 15.
3. the amino acid whose extracting method of non-irrigated lotus root according to claim 1, is characterized in that step 2) in concentrated pressure be controlled at 50-60kpa.
4. the amino acid whose extracting method of non-irrigated lotus root according to claim 1, it is characterized in that, step 3) in, in a cold soaking processing, concentrate is 1 with adding the ratio of ethanol: 10-1: 20, in the processing of secondary cold soaking, concentrate is 1 with adding the ratio of ethanol: 8-1: 15, and the temperature of cold soaking is controlled at 15-20 ℃.
5. the amino acid whose extracting method of non-irrigated lotus root according to claim 1, is characterized in that step 4) in alcohol reflux extract in the concentration of ethanol be 78%-90%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410310667.1A CN104207054A (en) | 2014-06-30 | 2014-06-30 | Method for extracting amino acids from Canna edulis |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410310667.1A CN104207054A (en) | 2014-06-30 | 2014-06-30 | Method for extracting amino acids from Canna edulis |
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| CN104207054A true CN104207054A (en) | 2014-12-17 |
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| CN201410310667.1A Pending CN104207054A (en) | 2014-06-30 | 2014-06-30 | Method for extracting amino acids from Canna edulis |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105379968A (en) * | 2015-11-19 | 2016-03-09 | 重庆三零三科技有限公司 | Extraction method of jerusalem artichoke leaf amino acid |
| CN112138065A (en) * | 2020-08-18 | 2020-12-29 | 中谱安信(杭州)检测科技有限公司 | Method for extracting amino acid from stramonium leaves |
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2014
- 2014-06-30 CN CN201410310667.1A patent/CN104207054A/en active Pending
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|---|---|---|---|---|
| JPS6147155A (en) * | 1984-08-14 | 1986-03-07 | Daiichi Seimo Kk | Production of amino acid |
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| CN101747216A (en) * | 2008-12-19 | 2010-06-23 | 中国科学院海洋研究所 | Method for extracting iodine-containing amino acid from fresh kelp |
| CN102531934A (en) * | 2010-12-24 | 2012-07-04 | 苏州宝泽堂医药科技有限公司 | Method for extracting amino acid from asparagus |
| CN103642862A (en) * | 2013-12-02 | 2014-03-19 | 济南开发区星火科学技术研究院 | Method for separating and extracting composite amino acid from hibiscus syriacus leaf |
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| CN105379968A (en) * | 2015-11-19 | 2016-03-09 | 重庆三零三科技有限公司 | Extraction method of jerusalem artichoke leaf amino acid |
| CN112138065A (en) * | 2020-08-18 | 2020-12-29 | 中谱安信(杭州)检测科技有限公司 | Method for extracting amino acid from stramonium leaves |
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