CN104204768B - 分选流式细胞仪 - Google Patents

分选流式细胞仪 Download PDF

Info

Publication number
CN104204768B
CN104204768B CN201380018973.1A CN201380018973A CN104204768B CN 104204768 B CN104204768 B CN 104204768B CN 201380018973 A CN201380018973 A CN 201380018973A CN 104204768 B CN104204768 B CN 104204768B
Authority
CN
China
Prior art keywords
sorting
drop
charge
fluid
droplet
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380018973.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN104204768A (zh
Inventor
托马斯·L·思拉舍
布鲁斯·G·贝利
埃里克·冯·塞根
杰夫瑞·W·戴杰尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Publication of CN104204768A publication Critical patent/CN104204768A/zh
Application granted granted Critical
Publication of CN104204768B publication Critical patent/CN104204768B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G01N15/1492—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties within droplets
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1021—Measuring mass of individual particles

Landscapes

  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrostatic Separation (AREA)
CN201380018973.1A 2012-02-09 2013-02-08 分选流式细胞仪 Active CN104204768B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261597022P 2012-02-09 2012-02-09
US61/597,022 2012-02-09
PCT/US2013/025308 WO2013119924A1 (en) 2012-02-09 2013-02-08 Sorting flow cytometer

Publications (2)

Publication Number Publication Date
CN104204768A CN104204768A (zh) 2014-12-10
CN104204768B true CN104204768B (zh) 2018-07-10

Family

ID=47750827

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380018973.1A Active CN104204768B (zh) 2012-02-09 2013-02-08 分选流式细胞仪

Country Status (9)

Country Link
US (1) US10190959B2 (enExample)
EP (1) EP2812671B1 (enExample)
JP (1) JP6192665B2 (enExample)
CN (1) CN104204768B (enExample)
AU (1) AU2013216878B2 (enExample)
ES (1) ES2776157T3 (enExample)
IN (1) IN2014DN06692A (enExample)
SG (1) SG11201404780VA (enExample)
WO (1) WO2013119924A1 (enExample)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10481069B2 (en) * 2011-01-03 2019-11-19 Cytonome/St, Llc Method and apparatus for monitoring and optimizing microfluidic particle sorting
WO2012119118A2 (en) 2011-03-03 2012-09-07 Life Technologies Corporation Sampling probes, systems, apparatuses, and methods
JP6447506B2 (ja) * 2013-10-16 2019-01-09 ソニー株式会社 粒子分取装置及び粒子分取方法
JP6136843B2 (ja) * 2013-10-17 2017-05-31 ソニー株式会社 粒子分取装置、粒子分取方法及びプログラム
US20150160246A1 (en) 2013-12-11 2015-06-11 Analiza, Inc. Devices and methods for determining and/or isolating cells such as circulating cancer or fetal cells
US9709556B2 (en) 2013-12-11 2017-07-18 Analiza, Inc. Devices and methods for determining and/or isolating circulating cancer cells
USD787701S1 (en) * 2014-03-21 2017-05-23 Intellicyt Corporation Flow cytometer system shelf unit
JP2016080563A (ja) * 2014-10-20 2016-05-16 日本光電工業株式会社 分析システム、分析装置
US10091279B2 (en) 2015-05-27 2018-10-02 FlowJo, LLC Wireless connected laboratory
US11254557B2 (en) 2016-05-17 2022-02-22 Sony Corporation Particle extraction apparatus and particle extraction method
US11016017B2 (en) 2016-06-10 2021-05-25 The Regents Of The University Of California Image-based cell sorting systems and methods
US20190257736A1 (en) * 2016-06-21 2019-08-22 Synelixis AB System and Method for Precision Deposition of Liquid Droplets
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN117143960A (zh) 2017-05-18 2023-12-01 10X基因组学有限公司 用于分选液滴和珠的方法和系统
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
EP3785015B1 (en) 2018-04-27 2024-06-26 Becton, Dickinson and Company Flow cytometers having enclosed droplet sorters with controlled aerosol content and methods of using the same
WO2020163023A1 (en) 2019-02-08 2020-08-13 Becton, Dickinson And Company Droplet sorting decision modules, systems and methods of use thereof
CN109916803A (zh) * 2019-02-27 2019-06-21 苏州朗如精密机械科技有限公司 一种流式细胞分析仪样本分选检测模块及其全自动分选检测方法
EP3956649A4 (en) * 2019-04-19 2023-01-04 Becton, Dickinson and Company UNDERSAMPLING FLOW CYTOMETRIC EVENT DATA
CN112730408B (zh) * 2020-12-24 2024-07-09 贝克曼库尔特生物科技（苏州）有限公司 液流检测系统和液流检测方法以及分选装置
CN115824736B (zh) * 2022-11-10 2023-10-20 东西分析仪器(天津)有限公司 一种微量样品点样头
CN115962995B (zh) * 2022-12-26 2023-07-04 中国科学院基础医学与肿瘤研究所(筹) 一种全自动微生物染色制片装置
CN118681609B (zh) * 2023-03-24 2026-02-13 深圳华大生命科学研究院 液滴微流控分选方法、分选系统、电子设备及存储介质

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4347935A (en) * 1979-05-16 1982-09-07 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for electrostatically sorting biological cells
JPS62167478A (ja) * 1985-11-29 1987-07-23 Shimadzu Corp 粒子分取装置
US4981580A (en) * 1989-05-01 1991-01-01 Coulter Corporation Coincidence arbitration in a flow cytomery sorting system
US5199576A (en) * 1991-04-05 1993-04-06 University Of Rochester System for flexibly sorting particles
US5483469A (en) * 1993-08-02 1996-01-09 The Regents Of The University Of California Multiple sort flow cytometer
US5617911A (en) 1995-09-08 1997-04-08 Aeroquip Corporation Method and apparatus for creating a free-form three-dimensional article using a layer-by-layer deposition of a support material and a deposition material
US6281018B1 (en) * 1998-02-26 2001-08-28 Coulter International Corp. Selective purification and enrichment sorting of flow cytometer droplets based upon analysis of droplet precursor regions
US6211477B1 (en) * 1998-02-26 2001-04-03 Becton Dickinson And Company Electrostatic deceleration system for flow cytometer
US6248590B1 (en) * 1998-02-27 2001-06-19 Cytomation, Inc. Method and apparatus for flow cytometry
US6079836A (en) * 1998-07-20 2000-06-27 Coulter International Corp. Flow cytometer droplet break-off location adjustment mechanism
US20030211009A1 (en) * 2001-05-18 2003-11-13 Buchanan Kris S. Rapid multi-material sample input system
EP1468266A4 (en) * 2002-01-22 2009-03-11 Beckman Coulter Inc ENVIRONMENTALLY COMPLETED SYSTEM FOR A FLOW CYTOMETER
US20110177547A1 (en) * 2004-12-10 2011-07-21 Arryx, Inc. Particle Sorting Using Fluid Streams
US8290625B2 (en) 2007-04-04 2012-10-16 Beckman Coulter, Inc. Flow cytometer sorter
US7691636B2 (en) * 2007-05-23 2010-04-06 Beckman Coulter, Inc. Method and apparatus for compensating for variations in particle trajectories in electrostatic sorter for flowcell cytometer
JP4990746B2 (ja) * 2007-12-14 2012-08-01 ベイバイオサイエンス株式会社 液体フローに含まれる生物学的粒子を分別する装置ならびにその方法
US8140300B2 (en) * 2008-05-15 2012-03-20 Becton, Dickinson And Company High throughput flow cytometer operation with data quality assessment and control
JP5446563B2 (ja) 2009-08-06 2014-03-19 ソニー株式会社 微小粒子分取装置、および該微小粒子分取装置を用いたフローサイトメーター
JP2011237201A (ja) 2010-05-06 2011-11-24 Sony Corp 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール
US8705031B2 (en) * 2011-02-04 2014-04-22 Cytonome/St, Llc Particle sorting apparatus and method
US9897530B2 (en) * 2011-08-25 2018-02-20 Sony Corporation Compensation of motion-related error in a stream of moving micro-entities

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Electrokinetically driven micro flow cytometers with integrated fiber optics for on-line cell/particle detection;Lung-Ming Fu et al.;《Analytica Chimica Acta》;20041231;第507卷;第163–169页 *
USAGE OF THE FLOW CYTOMETER-CELL SORTER;R.G, MILLER et al.;《Journal of Immunological Methods》;19811231;第47卷;第13-24页 *
流式细胞仪分选细胞技术;朱立平等;《中国医学科学院学报》;19881031;第10卷(第5期);第368-370页 *
流式细胞分选装置的研制与发展;周宏伟等;《生物物理学报》;19970930;第13卷(第3期);第512-518页 *

Also Published As

Publication number Publication date
CN104204768A (zh) 2014-12-10
EP2812671B1 (en) 2019-12-25
EP2812671A1 (en) 2014-12-17
AU2013216878A1 (en) 2014-08-28
US20150050688A1 (en) 2015-02-19
JP6192665B2 (ja) 2017-09-06
AU2013216878B2 (en) 2016-09-29
SG11201404780VA (en) 2014-09-26
IN2014DN06692A (enExample) 2015-05-22
ES2776157T3 (es) 2020-07-29
US10190959B2 (en) 2019-01-29
WO2013119924A1 (en) 2013-08-15
JP2015507204A (ja) 2015-03-05

Similar Documents

Publication Publication Date Title
CN104204768B (zh) 分选流式细胞仪
CN112304820B (zh) 流体组合物传感器设备及其使用方法
JP6102783B2 (ja) 粒子分取装置、粒子分取方法及びプログラム
JP6092132B2 (ja) 血球分析装置
CN105659069B (zh) 颗粒分取设备、颗粒分取方法与程序
Leary Ultra high‐speed sorting
EP3908824B1 (en) Optimized sorting gates
JPH06507244A (ja) 粒子を柔軟的に分類するシステム
US12019006B2 (en) Subsampling flow cytometric event data
US10201896B2 (en) Processing method, operation command generator, non-transitory computer-readable storage medium storing program, and processing system
CN103998915B (zh) 用于改进分选颗粒的产量的系统和方法
CN102144153B (zh) 用于分类、显示并探查生物数据的方法和装置
EP3882601B1 (en) Cell classification method, classification device, and program
CN112903569A (zh) 用于计算液滴延迟时间的系统和方法以及分选装置
CN114341617A (zh) 促进来自液滴分配器的受控颗粒沉积
Pérez-Vaquero et al. Electric charge limits on settled powders
CN119278364A (zh) 信息处理系统、信息处理方法、信息处理装置和程序
US20230073786A1 (en) Particle separation
Wong et al. Fluctuation-theorem method of measuring a particle's mass without knowing its shape or density
Beer et al. Passive chip-based droplet sorting
Semenov et al. Calculation of Process of Separation of a Single-Phae Dispersed System in a Horizontal Gravity Pneumatic Classifier
Steen et al. Dye exclusion artifact in flow cytometers
Broßell Instrument Development for Nanomaterial Risk Assessment
Tanik DECIPHERING CELL SORTING ELECTRONICS
Mehel The influence of the dispersion model and electrochemical forces on nanoparticle deposition in a turbulent flow

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant