CN104193513A - Rooting bacteria solution for fructus forsythiae cutting seedlings - Google Patents
Rooting bacteria solution for fructus forsythiae cutting seedlings Download PDFInfo
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Abstract
The invention discloses a rooting bacteria solution for fructus forsythiae cutting seedlings. Each liter of rooting bacteria solution comprises the following components by weight: 10-30 grams of bacillus subtilis powder, 1-2 grams of potassium dihydrogen phosphate, 0.5-1 gram of indometacin and the balance of water, wherein the bacillus subtilis content of the bacillus subtilis powder is 1*10<10>/gram-1*10<12>/gram; the bacillus subtilis is Bacillus Subtilis ZK1-18, and the preservation number of the bacillus subtilis is CGMCC No.9402. The rooting bacteria solution disclosed by the invention can promote the rooting of the fructus forsythiae cutting seedlings, shorten the rooting time, enhancing the rooting rate, strengthen the growth of the main roots of fructus forsythiae, promote the germination of branch roots and enhance the activity of the root system of the fructus forsythiae. The bacillus subtilis contained in the rooting bacteria solution is capable of loosening soil through the activity in the soil, so that the soil is well regulated in fertilizer maintenance, fertilizer supply, water retention, water supply and air permeability.
Description
Technical field
The invention belongs to microbial fertilizer technical field, be specifically related to a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation.
Background technology
The capsule of weeping forsythia (Forsythia suspensa (Thunb.) vahl) is the conventional bulk medicinal materials of China, and its good economic benefit, ecological benefits and the critical role in new drug development and new resources utilization are familiar with by increasing people; The capsule of weeping forsythia, afforesting and beautifying widespread use aspect city, is also considered to the rare fine tree species of sight-seeing agriculture and modern garden.Because the capsule of weeping forsythia is difficult for solidly, normal by tillering propagation in producing at present, but normal cutting propagation is difficult for taking root and survives, seedling propagation coefficient is low, and supply falls short of demand to make market nursery stock.
Summary of the invention
Technical problem to be solved by this invention is for above-mentioned the deficiencies in the prior art, and a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation is provided.This bacterium liquid of taking root can promote capsule of weeping forsythia cuttage seeding to take root, shorten rootage duration, improves rooting rate, and strong capsule of weeping forsythia main root growth, promotes lateral root to sprout, and improves capsule of weeping forsythia improving activity of root system.This subtilis taking root in bacterium liquid activity in soil can chessom, and fertilizer conservation, fertilizer, water conservation, water supply and the ventilation property of soil are all well regulated.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation, is characterized in that, take water as solvent, the every liter of bacterium liquid of taking root comprises subtilis bacterium powder 10g～30g, potassium primary phosphate 1g～2g, indomethacin 0.5g～1g; In described subtilis bacterium powder, the content of subtilis is 1 * 10
10individual/g～1 * 10
12individual/g; Described subtilis is Bacillus subtilis ZK1-18, deposit number CGMCC No.9402, preservation day is on June 30th, 2014, depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
Above-mentioned a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation, is characterized in that, in described subtilis bacterium powder, the content of subtilis is 1 * 10
11individual/g.
Above-mentioned a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation, is characterized in that, in the bacterium liquid of taking root, also comprises for dissolving the glycerine of indomethacin described in every liter.
Subtilis ZK1-18 of the present invention has following character:
Morphological specificity: shaft-like, size (0.5～0.8) μ m * (1.8～2.2) μ m is raw or near middle raw in gemma.Bacterium colony is circular, and white, surface drying have fold, tarnish, opaque, and edge is rough.
Physiological and biochemical property: gram positive bacterium.
The screening method of subtilis ZK1-18 of the present invention is:
Step 1, enrichment: from the following 5cm in Biotechnology Institute of school of Arts and Sciences Fructus Fici Orchard Soil top layer, Xi'an, Yanta District, Xi'an～10cm, gather soil sample 100g left and right, by adopt soil sample put into paper bag and take back laboratory separation.With sterile manner, take the triangular flask that soil sample 10.0g puts into the 250mL that contains 100.0mL enrichment medium (sterilizing), in 30 ℃, 140r/min constant-temperature table enrichment culture 5d; Then draw 5mL nutrient solution and again transfer in fresh enrichment medium, then carry out enrichment culture 3 times, each 5d; Consisting of of described enrichment medium: glucose 3.506g, peptone 0.83g, yeast extract paste 0.5g, potassium primary phosphate 0.35g, calcium carbonate 0.25g, Yelkin TTS 0.02g, distilled water 1000mL, Medium's PH Value 6.8～7.1,105KPa sterilizing 20min～25min;
Step 2, screening and purifying: the enrichment culture liquid after 3 enrichment culture in step 1 is pressed to decimal dilution method dilution, get 10
-3, 10
-4, 10
-5, 10
-6, 10
-75 each 0.1mL of dilution nutrient solution coat in organophosphorus solid plate substratum, 3 flat boards of each extent of dilution coating, be placed in cultivation 5d in 28 ℃ of constant incubators, the bacterial strain that can produce molten phosphorus circle (transparent circle) is phosphate-solubilizing bacteria, by the larger bacterial strain of the molten phosphorus circle purifying of repeatedly ruling on organophosphorus solid medium flat board, with purifying, obtain the purifying bacterial strain that form is consistent, then purifying bacterial strain is transferred on basic medium inclined-plane, in 28 ℃ of incubators, cultivate 2d, be placed in 4 ℃ of refrigerators and preserve stand-by; Consisting of of described basic medium: glucose 3.506g, peptone 0.83g, yeast extract paste 0.5g, potassium primary phosphate 0.35g, calcium carbonate 0.25g, agar 16g～20g, distilled water 1000mL; Consisting of of described organophosphorus solid plate substratum: basic medium, yolk liquid 60mL, Medium's PH Value nature; The method for making of organophosphorus solid plate substratum: the basic medium after 1000mL sterilizing is cooled to below 50 ℃, add immediately move into after the freshly prepared yolk liquid of 60mL dull and stereotyped; Yolk liquid preparation: clean egg shell with alcohol, cut brokenly egg two ends with scalper, yolk is flowed in sterilized Erlenmeyer flask after diffluence egg white, approximately add equivalent stroke-physiological saline solution and shake up;
Step 3, multiple sieve: will after the bacterial strain activation of preserving in step 2, obtain bacteria suspension, bacteria suspension is added drop-wise in organophosphorus solid plate substratum, the 1 μ L sterilized water of take is contrast, repeat 3 times, under 28 ℃ of conditions, cultivate 5d, with ruler, measure molten phosphorus loop diameter (D) and colony diameter (d), and according to the size that whether produces molten phosphorus circle and D/d value, tentatively determine the phosphorus decomposing ability (the larger phosphorus decomposing ability of D/d value is better) of bacterial strain; Then by the good inoculation of phosphorus decomposing ability in 50mL Meng Jinna organophosphorus liquid nutrient medium, take isopyknic sterilized water as contrast, repeat 3 times, shaking table is cultivated after (28 ℃, 120r/min) 7d, and the centrifugal 15min of 8000r/min, gets supernatant liquor 5mL, carry out ultrasonic disruption bacterial cell (200W, 20min), make it to discharge the available phosphorus in bacterial cell, with molybdenum antimony resistance colorimetric method, measure PO
3 -4content, preserves PO
3 -4the bacterial strain that content is the highest, called after ZK1-18; The preparation method of described Meng Jinna organophosphorus liquid nutrient medium is: to add in the Meng Jinna substratum after 1000mL sterilizing 0.2g with anhydrous alcohol solution after filtration with the Yelkin TTS of sterilizing, wherein consisting of of Meng Jinna substratum: glucose 10g, (NH
4)
2sO
40.5g, MgSO
47H
2o0.3g, NaCl0.3g, KCl0.3g, FeSO
40.03g, MnSO
4h
2o0.03g, distilled water 1000mL, 105KPa sterilizing 20min～25min.
The authentication method of subtilis ZK1-18 of the present invention is:
(1) Morphological Identification: the bacterial strain ZK1-18 screening is cultivated on agar plate, observe bacterial colony growth characteristics, note its shape, size, color, transparency, edge feature etc., and note down.With transfering loop picking list bacterium colony, carry out gramstaining, microscopy, records result simultaneously.
Result: ZK1-18 bacterial strain is shaft-like, size (0.5～0.8) μ m * (1.8～2.2) μ m is raw or near middle raw in gemma.Bacterium colony is circular, and white, surface drying have fold, tarnish, opaque, and edge is rough, and gramstaining is positive.
(2) Physiology and biochemistry is identified: Physiology and biochemistry experiment is carried out according to uncle's Jie Shi Bacteria Identification handbook (the 9th edition) method, the results are shown in following table.
The physiological and biochemical property of table 1 ZK1-18 bacterial strain
| Feature | ZK1-18 bacterial strain | Feature | ZK1-18 bacterial strain |
| Gelatin hydrolysis test | + | Fat splitting | － |
| VP reaction | + | Urea test | － |
| Catalase test | + | Indole test | + |
| Catalase | + | Glucose produces acid | + |
| Citrate test | － | Glucose aerogenesis | + |
| Starch Hydrolysis | + | ? | ? |
Note: in table, "+" is positive, "-" is negative.
(3) amplification of 16SrDNA and order-checking:
Step 1: bacteria total DNA is extracted: the ZK1-18 bacterium (3 * 10 of getting 1mL incubated overnight
8individual) bacterium liquid, put into the centrifuge tube of 1.5mL, under room temperature, the centrifugal 1min of 8000r/min, abandons supernatant; The massfraction of the resuspended 1.0mL of being deposited in is 0.85% NaCl solution, 4 ℃, the centrifugal 5min of 13000r/min, abandon supernatant, add the resuspended thalline of 400 μ L TE, add 20 μ L lysozyme solns (20mg/mL N,O-Diacetylmuramidase, 20mM Tris-HCl pH8,2.5mM EDTA, 1%TritonX-100) 37 ℃ spend the night; Add 400 μ L CTAB extracting solutions, shake up gently, 65 ℃ of water-bath 40min (every 10min jog once); Be cooled to room temperature, add mixed liquid (the V phenol: V chloroform=1:1), shake up gently 12000r/min, 4 ℃ of centrifugal 10min of 400 μ L phenol chloroforms; Move supernatant to the new centrifuge tube of 1.5mL, add mixed liquid (the V chloroform: V primary isoamyl alcohol=24:1), mix 12000r/min, 4 ℃ of centrifugal 10min of equal-volume chloroform isoamyl alcohol; Move supernatant to the new centrifuge tube of 1.5mL, add the freezing Virahol of equal-volume ,-20 ℃ of standing 60min precipitation DNA, 12000r/min, 4 ℃ of centrifugal 10min, abandon supernatant, add the ethanol that 500 μ L volume fractions are 75%, put upside down for several times, centrifugal, repeat 2 times; Be inverted centrifuge tube, dry DNA precipitation 10～15min; Add 200 μ L TE dissolution precipitations, add 20 μ L RNA enzymes (20mg/mL), 37 ℃ of insulation 30min, with the mixed liquid of isopyknic phenol chloroform (V phenol: V chloroform=1:1) and each extracting of chloroform once; Draw supernatant liquor, add the dehydrated alcohol of 1/10 volume 3mol/L NaAc (pH5.2) and 2 times of volumes, shake up gently to occurring flocks, the centrifugal 30min of 1000r/min, abandons supernatant liquor; With 75% washing with alcohol DNA, precipitate 2 times, air-dry; Add 30 μ L TE damping fluids ,-20 ℃ of preservations are stand-by; Adopting concentration is that 2% agarose detects the total DNA extracting, and clip size is in 13kb left and right;
The pcr amplification of step 2,16SrDNA: the total DNA that extracts in step 1 of take carries out pcr amplification as template, with bacterium universal primer 27F (SEQ ID NO.1): 5 '-AGAGTTTGATCCTGGCTCAG-3 '; 1492R (SEQ ID NO.2): 5 '-GGTTACCTTGTTACGACTT-3 ' amplification 16SrDNA, PCR reaction system (50 μ L) is: 2 * PCR Buffer25 μ L, DNA profiling 2 μ L, each 1 μ L of primer 2 7F (10 μ M) and 1492R (10 μ M), dNTP (10Mm) 1 μ L, 10 * Taq reaction Buffer5 μ L, Taq (5U/ μ L) 0.25 μ L, adds redistilled water to 50 μ L; PCR condition is: 94 ℃ of sex change 1min, and 50 ℃ of annealing 40s, 72 ℃ are extended 1.5min, 30 circulations;
Step 3, bacterial strain 16SrDNA sequential analysis and comparison: the product of pcr amplification in step 2 (about clip size 1.4kb) is checked order (by upper sea base health biotechnology company limited, having been assisted), and the associated clip sequence of the bacillus subtilis bacterial strain QD517 having delivered in the 16SrDNA portion gene sequence obtaining and GenBank has 99% homology.
Comprehensive Physiology and biochemistry is identified, 16SrDNA sequential analysis qualification result, identifies that bacterial strain ZK1-18 of the present invention is subtilis (Bacillus subitils).
The present invention compared with prior art has the following advantages:
1, the present invention prepares microbial fertilizer with subtilis ZK1-18, and technique is simple, with short production cycle, and cost is low.This bacterial strain comes from soil, and ecological safety is corrosion-free, harmless to people and animals animals and plants, environmentally safe, and application process is easy, and consumption is few, is easy to promote, be a kind of high-quality bacterium liquid of taking root.
2, the bacterium liquid of taking root of the present invention, can promote capsule of weeping forsythia cuttage seeding to take root, shorten rootage duration, improve rooting rate, the activity of the subtilis taking root in bacterium liquid in soil can chessom, and fertilizer conservation, fertilizer, water conservation, water supply and the ventilation property of soil are all well regulated.
3, in the present invention, contain special bacteria agent subtilis ZK1-18, can grow by strong capsule of weeping forsythia main root, promote lateral root to sprout, improve capsule of weeping forsythia improving activity of root system.
4, subtilis ZK1-18 of the present invention is the dominant strain screening with organic phosphorus sources, and bacterial strain can change the character of organophosphorus in growth and breeding process, and the component of organophosphorus is changed, and plays phosphate solubilization.
Below by embodiment, technical scheme of the present invention is described in further detail.
Embodiment
Embodiment 1
The bacterium liquid of taking root for capsule of weeping forsythia cutting propagation of the present embodiment, the every liter of bacterium liquid of taking root comprises subtilis bacterium powder 10g, potassium primary phosphate 1g, indomethacin 0.5g, for dissolving the glycerine (glycerine consumption is for dissolving indomethacin) of indomethacin, surplus is water; In described subtilis bacterium powder, the content of subtilis is 1 * 10
12individual/g; Described subtilis is Bacillus subtilis ZK1-18, deposit number CGMCC No.9402.
The preparation method of the bacterium liquid of taking root of the present embodiment comprises the following steps:
Step 1, ZK1-18 inoculation is cultivated in solid medium, culture temperature is 30 ℃, and incubation time is 36h～72h, obtains first class inoculum; Described solid culture based component is: glucose 3.506g, and peptone 0.83g, yeast extract paste 0.5g, potassium primary phosphate 0.35g, calcium carbonate 0.25g, agar 16g～10g, water 1000mL, Medium's PH Value is 7.0～7.2,105KPa, sterilizing 20min～25min;
Step 2, first class inoculum described in step 1 is inoculated in liquid nutrient medium and is cultivated, culture temperature is 30 ℃, and incubation time is 36h～72h, obtains second class inoculum; Described liquid culture based component is: glucose 3.506g, and peptone 0.83g, yeast extract paste 0.5g, potassium primary phosphate 0.35g, calcium carbonate 0.25g, water 1000mL, Medium's PH Value is 7.0～7.2,105KPa, sterilizing 20min～25min;
Step 3, second class inoculum described in step 2 is inoculated in fermention medium and carries out fermentation culture, culture temperature is 30 ℃, and incubation time is 36h～72h, obtains ZK1-18 bacterial strain fermentation liquor; Described fermentation culture based component is: glucose 3.506g, and peptone 0.83g, yeast extract paste 0.5g, potassium primary phosphate 0.35g, calcium carbonate 0.25g, water 1000mL, Medium's PH Value is 7.0～7.2,105KPa, sterilizing 20min～25min;
1000rpm is centrifugal for step 4, employing, and thalline is collected in washing, and drying thalline to subtilis ZK1-18 content is 1 * 10
12individual/g, obtains subtilis bacterium powder;
Step 5, indomethacin is dissolved with glycerine, then by proportioning, the indomethacin by subtilis bacterium powder, after dissolving and potassium primary phosphate are added to the water and mix, and bacterium liquid obtains taking root.
Embodiment 2
The bacterium liquid of taking root for capsule of weeping forsythia cutting propagation of the present embodiment, the every liter of bacterium liquid of taking root comprises subtilis bacterium powder 30g, potassium primary phosphate 1.5g, indomethacin 1g, for dissolving the glycerine (glycerine consumption is for dissolving indomethacin) of indomethacin, surplus is water; In described subtilis bacterium powder, the content of subtilis is 1 * 10
10individual/g; Described subtilis is Bacillus subtilis ZK1-18, deposit number CGMCC No.9402.
The preparation method of the bacterium liquid of taking root of the present embodiment is identical with embodiment 1.
Embodiment 3
The bacterium liquid of taking root for capsule of weeping forsythia cutting propagation of the present embodiment, the every liter of bacterium liquid of taking root comprises subtilis bacterium powder 15g, potassium primary phosphate 1.5g, indomethacin 0.8g, for dissolving the glycerine (glycerine consumption is for dissolving indomethacin) of indomethacin, surplus is water; In described subtilis bacterium powder, the content of subtilis is 1 * 10
11individual/g; Described subtilis is Bacillus subtilis ZK1-18, deposit number CGMCC No.9402.
The preparation method of the bacterium liquid of taking root of the present embodiment is identical with embodiment 1.
Embodiment 4
The bacterium liquid of taking root for capsule of weeping forsythia cutting propagation of the present embodiment, the every liter of bacterium liquid of taking root comprises subtilis bacterium powder 25g, potassium primary phosphate 2g, indomethacin 0.5g, for dissolving the glycerine (glycerine consumption is for dissolving indomethacin) of indomethacin, surplus is water; In described subtilis bacterium powder, the content of subtilis is 1 * 10
11individual/g; Described subtilis is Bacillus subtilis ZK1-18, deposit number CGMCC No.9402.
The preparation method of the bacterium liquid of taking root of the present embodiment is identical with embodiment 1.
The phosphorus decomposing test of subtilis of the present invention:
1, transparent circle method is measured the phosphorus decomposing ability test of ZK1-18 bacterial strain
The bacteria suspension of the ZK1-18 bacterial strain having activated is added drop-wise to organophosphorus solid plate substratum central authorities, the 1 μ L sterilized water of take is contrast, under 28 ℃ of conditions, cultivate 5d, with ruler, measure molten phosphorus loop diameter (D) and colony diameter (d), and according to the size that whether produces molten phosphorus circle and D/d value, determine and the phosphorus decomposing ability of bacterial strain the results are shown in Table 2.
Table 2 transparent circle method is measured the phosphorus decomposing ability of ZK1-18 bacterial strain
| ? | Molten phosphorus loop diameter D/mm | Colony diameter d/mm | Ratio D/d |
| ZK1-18 bacterial strain | 13.3mm | 4.8mm | 2.77 |
| Contrast | 0 | 0 | 0 |
2, the titanium pigment content of ZK1-18 bacterial strain fermentation liquor test
Adopt liquid shaking bottle culture method, in 150mL triangular flask, 1mL ZK1-18 bacterial suspension inoculation, in 50mL Meng Jinna organophosphorus liquid nutrient medium, be take and connect isopyknic sterilized water as contrast, and shaking table is cultivated after (28 ℃, 120r/min) 7d, the centrifugal 15min of 8000r/min, get supernatant liquor 5mL, carry out ultrasonic disruption bacterial cell (200W, 20min), make it to discharge the available phosphorus in bacterial cell, with molybdenum antimony resistance colorimetric method, measure PO
3 -4content, the results are shown in Table 3.
The titanium pigment content of table 3 ZK1-18 bacterial strain fermentation liquor
| ? | Titanium pigment content (mg/L) |
| ZK1-18 bacterial strain | 18.31mg/L |
| Contrast | 1.03mg/L |
Application method and the effect of the bacterium liquid of taking root of the present invention:
The green branch of the capsule of weeping forsythia without disease and pest is enriched in the growth gathering then, cuttings is cut into long 10cm～15cm, guaranteeing has 2～3 to grow substantial bud in cuttings, and in cuttings, clip cuts flat, and lower clip is near the base portion 1cm left and right of last bud of cuttings, and cross base portion and cut inclined-plane at 45 °, capsule of weeping forsythia cuttings is divided into 3 groups, every group of 50 branches, then 1 group immerses of the present invention taking root in bacterium liquid, process the long 3cm of cuttings base portion, soak 1h; Another two groups immerse respectively in clear water and ABT (100mg/L) liquid, process the long 3cm of cuttings base portion, soak 1h; Temperature is controlled at 25 ℃, before cuttage, matrix in bed is irrigated with clear water, by seeding row spacing 3cm * 3cm, extrudes cuttage line, uses punch tool perforating, and cuttings inclined-plane inserts downwards in soil, with the 3cm cuttage degree of depth, is advisable; Insert the rear compacting that conveniently jack press...withed one's finger, matrix and cuttings are connected airtight mutually; After cuttage, carry out immediately atomizing spray, make seedbed moist; Setting is adjusted in the different times that atomizing spray intensity is taken root and weather conditions in time; In cuttage 1d～15d, general 10min spray is 1 time, and 2min sprays at every turn; Night, 2h spray was 1 time, after 15d～25d, progressively extended the spray intervals time, to the every 1h spray of 35d～45d 1 time, and 1 time (stop night) of the every 4h spray of 55d～65d, hardening 10d～15d can transplant.Insert latter 25 days, 35 days, 50 days investigation rooting rates, to insert, within latter 65 days, in every group, randomly draw 30 strain investigation rooting rate and the quantity of taking root, the results are shown in Table 4.
Table 4 is the capsule of weeping forsythia green branch rooting rate and the quantity statistics of taking root after treatment
| ? | Rooting rate | The number of on average taking root |
| The bacterium liquid of taking root of embodiment 1 | 87.3％ | Article 14.2, |
| The bacterium liquid of taking root of embodiment 2 | 94.3％ | Article 16.0, |
| ABT contrast | 72.0％ | Article 10.1, |
| Clear water contrast | 43.3％ | 5.6 bar |
The green branch of the capsule of weeping forsythia without disease and pest is enriched in the growth that gathers life in 2 years, cuttings is cut into long 10cm～15cm, and guaranteeing has 2～3 to grow substantial bud in cuttings, and in cuttings, clip cuts flat, lower clip is near the base portion 1cm left and right of last bud of cuttings, and cross base portion and cut inclined-plane at 45 °, capsule of weeping forsythia cuttings is divided into 3 groups, every group of 50 branches, cuttings is placed in water and is soaked 1 hour, then 1 group immerses of the present invention taking root in bacterium liquid, processes the long 3cm of cuttings base portion, soaks 1h; Another two groups immerse respectively in clear water and ABT (100mg/L) liquid, process the long 3cm of cuttings base portion, soak 1h; Temperature is controlled at 25 ℃, before cuttage, matrix in bed is irrigated with clear water, by seeding row spacing 3cm * 3cm, extrudes cuttage line, uses punch tool perforating, and cuttings inclined-plane inserts downwards in soil, with the 3cm cuttage degree of depth, is advisable; Insert the rear compacting that conveniently jack press...withed one's finger, matrix and cuttings are connected airtight mutually; After cuttage, carry out immediately atomizing spray, make seedbed moist; Setting is adjusted in the different times that atomizing spray intensity is taken root and weather conditions in time; In cuttage 1d～15d, general 10min spray is 1 time, and 2min sprays at every turn; Night, 2h spray was 1 time, after 15d～25d, progressively extended the spray intervals time, to the every 1h spray of 35d～45d 1 time, and 1 time (stop night) of the every 4h spray of 55d～65d, hardening 10d～15d can transplant.Insert latter 25 days, 35 days, 50 days investigation rooting rates, to insert, within latter 65 days, in every group, randomly draw 30 strain investigation rooting rate and the quantity of taking root, the results are shown in Table 5.
Table 5 is the capsule of weeping forsythia green branch rooting rate and the quantity statistics of taking root after treatment
| ? | Rooting rate | The number of on average taking root |
| The bacterium liquid of taking root of embodiment 3 | 82.3％ | 8.3 bar |
| The bacterium liquid of taking root of embodiment 4 | 82.6％ | 8.8 bar |
| ABT contrast | 56.2％ | 5.6 bar |
| Clear water contrast | 37.5％ | 3.2 bar |
From table 4 and table 5, can find out, adopt the bacterium liquid of taking root of the present invention, can promote capsule of weeping forsythia cuttage seeding to take root, shorten rootage duration, improve rooting rate, strong capsule of weeping forsythia main root growth, promotes lateral root to sprout, and improves capsule of weeping forsythia improving activity of root system.
The above; it is only preferred embodiment of the present invention; not the present invention is done to any restriction, every any simple modification of above embodiment being done according to invention technical spirit, change and equivalent structure change, and all still belong in the protection domain of technical solution of the present invention.
Claims (3)
1. for the bacterium liquid of taking root of capsule of weeping forsythia cutting propagation, it is characterized in that, take water as solvent, the every liter of bacterium liquid of taking root comprises subtilis bacterium powder 10g～30g, potassium primary phosphate 1g～2g, indomethacin 0.5g～1g; In described subtilis bacterium powder, the content of subtilis is 1 * 10
10individual/g～1 * 10
12individual/g; Described subtilis is Bacillus subtilis ZK1-18, deposit number CGMCC No.9402.
2. a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation according to claim 1, is characterized in that, in described subtilis bacterium powder, the content of subtilis is 1 * 10
11individual/g.
3. a kind of bacterium liquid of taking root for capsule of weeping forsythia cutting propagation according to claim 1, is characterized in that, in the bacterium liquid of taking root, also comprises for dissolving the glycerine of indomethacin described in every liter.
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| CN110291892A (en) * | 2019-07-30 | 2019-10-01 | 山西省农业科学院农业科技信息研究所 | A kind of synthetic method for planting for preventing and treating Fructus Forsythiae pest and disease damage |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372435A (en) * | 2008-10-08 | 2009-02-25 | 丁剑兰 | Bio-bacterium fertilizer synergistic additive and preparation thereof |
| CN103910578A (en) * | 2014-04-04 | 2014-07-09 | 山东丰本生物科技股份有限公司 | Rooting nutritional type microbial fertilizer, and preparation method and applications thereof |
-
2014
- 2014-08-22 CN CN201410419181.1A patent/CN104193513B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372435A (en) * | 2008-10-08 | 2009-02-25 | 丁剑兰 | Bio-bacterium fertilizer synergistic additive and preparation thereof |
| CN103910578A (en) * | 2014-04-04 | 2014-07-09 | 山东丰本生物科技股份有限公司 | Rooting nutritional type microbial fertilizer, and preparation method and applications thereof |
Non-Patent Citations (1)
| Title |
|---|
| 孙全根: "吲哚美辛对番茄插条生根的影响", 《荷泽师专学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110291892A (en) * | 2019-07-30 | 2019-10-01 | 山西省农业科学院农业科技信息研究所 | A kind of synthetic method for planting for preventing and treating Fructus Forsythiae pest and disease damage |
| CN110291892B (en) * | 2019-07-30 | 2021-06-29 | 山西省农业科学院农业科技信息研究所 | Comprehensive cultivation method for preventing and treating diseases and insect pests of fructus forsythiae |
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|---|---|
| CN104193513B (en) | 2016-07-20 |
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