CN104190485A - Double-layer filter element for suction head of pipettor - Google Patents
Double-layer filter element for suction head of pipettor Download PDFInfo
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- CN104190485A CN104190485A CN201410473075.1A CN201410473075A CN104190485A CN 104190485 A CN104190485 A CN 104190485A CN 201410473075 A CN201410473075 A CN 201410473075A CN 104190485 A CN104190485 A CN 104190485A
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Abstract
The invention discloses a double-layer filter element for a suction head of a pipettor. The double-layer filter element for the suction head of the pipettor is characterized in that a lower filtering layer of the double-layer filter element is made of pure UHMW-PE or HDP, and functional materials are added into an upper filtering layer outside materials used for the lower filtering layer and contain one or more of water swelling agents, activated carbon and dyes; the pore diameters of the upper filtering layer and the lower filtering layer of the double-layer filter element are 1-100 um, which can be consistent; the overall thickness of the double-layer filter element is 2.0-8.0 mm. Through the double-layer filter element, various application demands like the absorption of dangerous and highly-infected samples, the separation of smaller matters, automatic identification, product personalization and the like can be met.
Description
Technical field
The invention belongs to experiment consumptive material field, relate to a kind of double-deck filter core for suction pipette head.
Background technology
Pipettor is that the conventional liquid in laboratory shifts and sample suction tool, and supporting suction nozzle (claiming again suction nozzle) is supporting consumable material with it.Suction nozzle uses polypropylene (PP) injection molded conventionally, has 10 μ L, 100 μ L, 200 μ L, 1000 μ L and 5mL equal-specification.Pipettor is the long-term precision equipment using, and suction nozzle is disposable use.When imbibition, the piston apparatus of pipettor presses down, and releases the air in sleeve, when pine removes pressure, forms the negative pressure of certain volume in sleeve, and the suction nozzle being connected with sleeve has just been drawn the liquid of certain volume.Liquid absorption accurately depend on following factor: the volume of air that the first is released in sleeve is wanted accurately, and the volume of air of release equals imbibition volume.It two is that the air flue that forms of negative pressure is unobstructed, can not have too large resistance, and high-drag meeting makes liquid absorption more on the low side than what estimate.It three is that the viscosity of liquid can not be too large, and viscosity also can make too greatly liquid absorption more on the low side than what estimate.
In the process of imbibition, the disturbance of liquid, comprise mobile, splash, wall built-up, blow out the actions such as remaining liquid and can produce aerosol (aerosol).Aerosol is disperseed and be suspended in the colloidal dispersion system forming in gas medium by solid or liquid fine particle, solid and liquid fine particle size are 0.001～100 μ m, comprise drop, bacterium, virus, mycoplasma, DNA, RNA, ultramicron molecule, such as the small pieces segment DNA of <100bp, the reagent molecule that some are special etc.Aerosol, along with the passage that negative pressure forms enters pipettor, finally spreads by two kinds of approach.The first is, while drawing next sample, to enter next sample, is conventionally called sample cross contamination; The second is to be diffused in air, when sample is dangerous sample, can cause adverse effect to operator, such as when drawing Ebola and AIDS virus sample or active sample.
The target of existing filter core suction nozzle design is mainly to avoid aerocolloidal cross pollution.Along with the use of molecular biological development, particularly PCR (PCR) technology, the DNA of single copy can pollute next sample, and aerosol usually can cause false positive to the pollution of testing environment, causes the erroneous judgement of clinical diagnosis.Filter core suction nozzle can effectively intercept aerosol, reduces sample cross contamination.In PCR experiment, in routine, use.
The filter core that existing filter core suction nozzle is used uses ultra-high molecular weight polyethylene (UHMW-PE) or high density polyethylene (HDPE) (HDPE) to produce conventionally.UHMW-PE is strong-hydrophobicity material, and water absorption rate <0.01%, lower than 6 times of polypropylene, lower than 100 times of nylon, is to intercept aerocolloidal optimal material.When filter core is produced, choose a certain size UHMW-PE particle, be seated in porous mold, pressure sintering, puts up a bridge between particle and forms mazy duct.Mean pore size is generally 10 μ m or 20 μ m.Filter core has following requirement conventionally.The firstth, material is pure, can not affect the performance of sample; The secondth, without RNase, DNase, PCR inhibitor, pollute; The 3rd is that vapour lock is little, and imbibition is smooth.
In order to increase aerocolloidal barriering effect, do not affect again the fluency of imbibition.There are following two progress:
First progress is that patent US201213408462 and patent JP2011252693 disclose a kind of double-deck filter core.Double-deck filter core is produced by UHMW-PE, and lower floor's filter course aperture is 20-50 μ m, and filter course aperture, upper strata is less, is less than 20 μ m.This kind of apparent benefit of filter core is that upper and lower filter course forms an efficient double barrier.Lower floor's filter course is in sample side, and aperture is large, mainly holds back drop, spatters liquid and air suspension thing.Upper strata filter course, aperture is little, even can effectively filter biomolecule.Yet it is single that this bilayer filter core intercepts principle, there is the potential risk that sees through; In order to realize aerocolloidal effective obstruct, levels filter core adopts different pore size, causes processing difficulties, production cost higher.
Second progress is the chance water seal filter core (self-enclosed filter core) that applicant's comma biotech firm releases, and can guarantee that aerosol and solution can not see through band filter core suction nozzle, really guarantee that pipettor can be by sample contamination.No matter do nucleic acid amplification experiment, still shift radiation isotope, virus, serology sample, meet water seal filter core suction nozzle and can not only guarantee that experimental result is reliable, and effectively protect instrument and researcher's safety.
In sum, existing several filter cores are compared as follows:
1, the filter core in common single aperture.Intercepting aerocolloidal principle is hydrophobic effect, and barriering effect depends on the selection of aperture and filter core thickness.Need to be between vapour lock and barriering effect balance, select the appropriate person of effect.The filter core that experiment showed, single aperture can not be stopped cross pollution, and pollutant still can pass through filter core sometimes, causes cross pollution.
2, the double-deck filter core in two kinds of apertures.Intercepting aerocolloidal principle is hydrophobic effect, and vapour lock and barriering effect have been taken into account in two kinds of apertures, have improved barriering effect.Shortcoming is that obstruct principle is single, has the potential risk that sees through, and production cost is high.
3, meet water seal filter core.Intercepting aerocolloidal principle is two kinds, and a kind of is hydrophobic effect, and a kind of is liquid fine particle in direct adsorbed gas colloidal sol, such as drop, steam and wrappage thereof.Meet water seal filter core and can also prevent liquid overshoot.Shortcoming is that the material of sample side is impure, and the sealer in filter core may touch sample, on sample, may cause potential impact.
Existing suction nozzle is with the purest material, to produce as far as possible by the design concept of filter core, avoids filter core to Sample producing potential impact.So do not develop new filter core technology in for a long time.Suction nozzle is molecular biology experiment with the topmost application of filter core, complicated along with what apply, proposes more and more higher requirement, such as:
Example one: draw dangerous sample, such as Ebola virus, AIDS virus sample or radioactivity sample, what mainly consider is the protection to operator, and the importance of protection cross pollution drops to second, usually needs the function of liquid overshoot protection.
Example two: detect the dissociative DNA in blood plasma, and the cross pollution that prevents PCR primer.DNA fragmentation is <100bp usually, even 20bp.To intercepting aerocolloidal effect, new requirement has been proposed.If use merely hydrophobic effect, the filter core aperture needing can be less, is low to moderate 1-5 μ m, the air drag while greatly having increased imbibition.
Example three: automatic fluid work station is more and more general.When automatic sample, need liquid level to identify to improve reliability.UHMW-PE is nonconducting, makes identification difficulty.
Example four: the sign of product and identification.Different brands all wishes that the product of oneself has unique feature, such as different colors.Present filter core suction nozzle, the part that may contact with sample is all virgin material, and suction nozzle itself is transparent polypropylene, and filter core is white UHMW-PE, and color is single.
In sum, existing pipettor filter core, still can not meet application needs completely.
Summary of the invention
The object of this invention is to provide a kind of double-deck filter core for pipettor, to meet various application demands, dangerous such as drawing, the high sample infecting, intercepts more small material, automation identification, personalization of product etc.Object of the present invention is achieved through the following technical solutions.
A double-deck filter core for pipettor, is characterized in that, pure UHMW-PE or the HDPE making for filter course of described double-deck filter core lower floor, adds functional mass beyond the material that upper strata filter course is used at lower floor's filter course.Definition to double-deck filter core levels in the present invention, be that one deck that approaches the sample that pipettor draws be lower floor, apart from sample that one deck far away, be upper strata.Lower floor's filter course has two effects, and the first intercepts aerosol by hydrophobic effect, and it two is that material is pure, reduces the potential impact to sample while using.The effect of upper strata filter core has two, and the first intercepts aerosol by hydrophobic effect, and it two is to provide the function relevant with added functional mass, such as increase adsorptivity, or raising identity.
Further, the functional mass that upper strata filter course adds, comprises and meets one or more in water swelling agent, active carbon, dyestuff.Meet water swelling agent and can realize chance water seal, prevent liquid overshoot, for example, when drawing dangerous sample (Ebola virus, AIDS virus, radioactive sample) effective Protection person.Active carbon possesses adsorption capacity, can further intercept aerosol, can also make filter core have certain electric conductivity simultaneously, to realize the identification of automatic fluid work station automatic sample.Dyestuff can make filter core according to manufacturer need to present colourful color, be conducive to product identification and personalization.
Further, the aperture of the levels filter core of described double-deck filter core is 1-100 μ m.The aperture of levels filter core can be consistent, also can be as prior art lower floor aperture large, aperture, upper strata is little or lower floor aperture is little, aperture, upper strata is large.
Further, the preferred aperture, aperture of the levels filter core of described double-deck filter core is consistent.The consistent advantage in levels filter core aperture is that simple process during the manufacturing, is conducive to reduce costs.
Further, described double-deck filter core integral thickness is 2.0-8.0mm, preferably 3.0-5.0mm.
A double-deck filter core for pipettor, is characterized in that, pure UHMW-PE or the HDPE making for filter course of described double-deck filter core lower floor, adds functional mass beyond the material that upper strata filter course is used at lower floor's filter course; The functional mass that upper strata filter course adds, comprises one or more of meeting water swelling agent (sealer), active carbon, dyestuff; The aperture of the levels filter core of described double-deck filter core is 1-100 μ m, and the aperture of the levels filter core of described double-deck filter core is consistent; Described double-deck filter core integral thickness is 3.0-5.0mm.
Double-deck filter core provided by the present invention has more function with respect to the common single aperture of prior art filter core, the different filter cores in levels aperture, individual layer chance water seal filter core and individual layer active carbon filter core, simultaneously neither common single aperture filter core and individual layer meet the simple superposition of water seal filter core or two filter core functions of individual layer active carbon filter core, its beneficial effect embodies in the following areas.
The first, the barriering effect of double-deck filter core is better than individual layer filter core.Lower floor's filter course, owing to only having hydrophobic effect, can be called weak barrier layer; , containing functional mass, there are hydrophobic effect and suction-operated in upper strata, is called strong barrier layer.When having aerosol, weak barrier layer has intercepted a large amount of aerosols, and the aerosol of trace enters strong barrier layer, by two kinds, intercepts mechanism, thoroughly blocking-up.Example one: when same aperture, upper strata is containing the double-deck filter core of active carbon, and barriering effect is better than the individual layer filter core of non-activity charcoal.Compare individual layer active carbon filter core, do not worry that again active carbon may have influence on solution property, or active carbon drops in sample when imbibition.Example two: when same aperture, upper strata is containing the double-deck filter core of sealer, and barriering effect is better than the individual layer filter core without sealer.Compare individual layer and meet water seal filter core, do not worry that again sealer may have influence on solution property, or sealer drops in sample when imbibition.
The second, double-deck filter core also can be understood as below adding the filter core of functional mass and has added separation layer, has avoided the defect of independent use.Such as using and meet water seal filter core separately, the material that shortcoming is filter core is impure, and the sealer in filter core may touch sample, on sample, may cause potential impact.If increase UHMW-PE that one deck is pure as separation layer, avoided independent use to meet the defect of water seal filter core.Use separately for another example active carbon filter core, shortcoming is that active carbon may drop in sample, on sample, may cause potential impact.If increase UHMW-PE that one deck is pure as separation layer, avoided the defect of independent use active carbon filter core.
The 3rd, cost advantage.The different double-deck filter core cost in upper and lower filter course aperture is high, and agglomerated material requires high, and the difference of pore size needs to optimize, and causes cost to rise.Double-deck filter core of the present invention, because the aperture difference to upper and lower filter course is without specific (special) requirements, cost is low.Compare common filter core, the UHMW-PE of upper and lower filter course is just the same, just in the filter course of upper strata, has added functional mass, and cost rises very little.
The 4th, the assembly structure of double-deck filter core and two stack filter cores is different.Two stack filter cores have space, and when aerosol penetrates, obstruct process restarts, and cause barriering effect to decline.And double-deck filter core has the region of merging mutually of an intersection, aerosol has increased a kind of blocking-up mechanism to this position, and blocked force strengthens, and effect is fine.
The 5th, the assembly difficulty of double-deck filter core and two stack filter cores is different.Filter core needs applicable thickness diameter Bizet convenient for assembly conventionally.Two filter cores, can be than thin one times with double-deck filter core, and thickness can be less than 2mm conventionally, and external diameter and suction nozzle fit clearance easily produce, impact effect, and the cost of assembling also can double.Double-deck filter core of the present invention can as required, make the thickness <1mm of certain layer; And during two common filter cores stacks, if can cannot assemble after some filter core <1mm.The preferential 3.0mm-5.0mm of double-deck filter core integral thickness, external diameter and suction nozzle coordinate gapless.
The 6th, the new function that double-deck filter core provides individual layer filter core not possess.For example upper strata filter course can be sneaked into suitable pigment, forms abundant color identification; Can add active carbon to increase absorption and electric conductivity.
Accompanying drawing explanation
Fig. 1 is the common single aperture of prior art filter core schematic diagram.
Fig. 2 is the inconsistent double-deck filter core schematic diagram in the upper and lower filter course of prior art aperture.
Fig. 3 double-deck filter core schematic diagram of the present invention.
Reference numeral: the skeleton particle (UHMW-PE or HDPE) of the common single aperture of 1-prior art filter core, filter core hole, the common single aperture of 2-prior art, the skeleton particle (UHMW-PE or HDPE) of the upper and lower filter course of 3-prior art aperture inconsistent double-deck filter core lower floor filter course, the hole of the upper and lower filter course of 4-prior art aperture inconsistent double-deck filter core lower floor filter course, the skeleton particle (UHMW-PE or HDPE) of inconsistent double-deck filter core upper strata, the upper and lower filter course of 5-prior art aperture filter course, filter course hole, inconsistent double-deck filter core upper strata, the upper and lower filter course of 6-prior art aperture, the skeleton particle (UHMW-PE or HDPE) of the double-deck filter core of 7-the present invention lower floor filter course, the double-deck filter core lower floor's filter course of 8-the present invention hole, the skeleton particle (UHMW-PE or HDPE mixed function material) of the double-deck filter core of 9-the present invention upper strata filter course, filter course hole, the double-deck filter core upper strata of 10-the present invention.
The specific embodiment
Embodiment 1
The double-deck filter core of active carbon
The double-deck filter core thickness of active carbon is 4mm, minute two-layer up and down, and thickness is 2mm.Upper and lower filter course all uses particle diameter 100-200 object UHMW-PE powder sintered, adds especially the active carbon (300-1000 order) of 5% mass ratio in the UHMW-PE of upper strata filter course powder.The aperture of upper and lower filter course is consistent.
Embodiment 2
Meet the double-deck filter core of water seal
Meeting the double-deck filter core thickness of water seal is 4mm, minute two-layer up and down, and lower floor's filter course thickness is 1.5mm, and the thickness of upper strata filter course is 2.5mm.Lower floor's filter course is powder sintered with particle diameter 100-200 object UHMW-PE, and upper strata filter course is powder sintered with particle diameter 200-300 object UHMW-PE, adds the sealer swollen resin of 1% mass ratio in the UHMW-PE powder of upper strata.The aperture of upper and lower filter course is different, and aperture, large upper strata, lower floor aperture is little.
Embodiment 3
Double-colored filter core
Double-colored filter core thickness is 3.0mm, minute two-layer up and down, and thickness is 1.5mm.Upper and lower filter course all uses particle diameter 100-200 object UHMW-PE powder sintered, the ferric oxide red powder of adding 0.5% mass ratio especially in the UHMW-PE of upper strata filter course powder.The aperture of upper and lower filter course is consistent.
Embodiment 4
Double-colored filter core
Double-colored filter core thickness is 5.0mm, minute two-layer up and down, and upper strata filter course thickness is 0.5mm, and lower floor's filter course thickness is 4.5mm.Upper and lower filter course all uses particle diameter 100-300 object UHMW-PE powder sintered, the ferric oxide red powder of adding 0.5% mass ratio especially in the UHMW-PE of upper strata filter course powder.The aperture of upper and lower filter course is consistent.
Embodiment 5
Multifunction double-layer filter core
Multifunction double-layer filter core thickness is 4mm, minute two-layer up and down, and thickness is 2mm.Upper and lower filter course all uses particle diameter 100-200 object HDPE powder sintered, adds especially active carbon (300-1000 order), the sealer swollen resin of 1% mass ratio and the ferric oxide red powder of 0.5% mass ratio of 4% mass ratio in the HDPE of upper strata filter course powder.The aperture of upper and lower filter course is consistent.
Claims (6)
1. for a double-deck filter core for pipettor, it is characterized in that, pure UHMW-PE or the HDPE making for filter course of described double-deck filter core lower floor, adds functional mass beyond the material that upper strata filter course is used at lower floor's filter course.
2. double-deck filter core according to claim 1, is characterized in that, the functional mass that upper strata filter course adds comprises and meets one or more in water swelling agent, active carbon, dyestuff.
3. double-deck filter core according to claim 1, is characterized in that, the aperture of the levels filter core of described double-deck filter core is 1-100 μ m.
4. double-deck filter core according to claim 1, is characterized in that, the aperture of the levels filter core of described double-deck filter core is consistent.
5. double-deck filter core according to claim 1, is characterized in that, described double-deck filter core integral thickness is 2.0-8.0mm, preferably 3.0-5.0mm.
6. for a double-deck filter core for pipettor, it is characterized in that, pure UHMW-PE or the HDPE making for filter course of described double-deck filter core lower floor, adds functional mass beyond the material that upper strata filter course is used at lower floor's filter course; The functional mass that upper strata filter course adds, comprises one or more of meeting water swelling agent, active carbon, dyestuff; The aperture of the levels filter core of described double-deck filter core is 1-100 μ m, and the aperture of the levels filter core of described double-deck filter core is consistent; Described double-deck filter core integral thickness is 3.0-5.0mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410473075.1A CN104190485A (en) | 2014-09-16 | 2014-09-16 | Double-layer filter element for suction head of pipettor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410473075.1A CN104190485A (en) | 2014-09-16 | 2014-09-16 | Double-layer filter element for suction head of pipettor |
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| CN201410473075.1A Pending CN104190485A (en) | 2014-09-16 | 2014-09-16 | Double-layer filter element for suction head of pipettor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110785228A (en) * | 2018-10-29 | 2020-02-11 | 深圳逗点生物技术有限公司 | Porous modified adsorbent for solid phase extraction, preparation method thereof and solid phase extraction device |
| CN110812942A (en) * | 2019-12-20 | 2020-02-21 | 严润达 | Sewage trickling treatment device with disc-shaped filter element |
| CN112827523A (en) * | 2021-01-08 | 2021-05-25 | 上海金鑫生物科技有限公司 | Ultrahigh molecular polyethylene filter containing activated carbon |
| CN115304848A (en) * | 2022-08-19 | 2022-11-08 | 智滤(苏州)材料科技有限公司 | Formula and manufacturing process of color-developing water-blocking filter element |
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| WO1999000193A1 (en) * | 1997-06-30 | 1999-01-07 | Rainin Instrument Co., Inc. | Improved membrane filter pipette tip |
| CN2566257Y (en) * | 2002-09-09 | 2003-08-13 | 黄一平 | Anti-pollution suction head for micro liquid suction device |
| US20090220386A1 (en) * | 2008-02-29 | 2009-09-03 | Ferri Joseph E | Porous Sealing Material |
| CN101528824A (en) * | 2006-08-18 | 2009-09-09 | 珀雷克斯公司 | Sintered polymeric materials and applications thereof |
| US20120156114A1 (en) * | 2005-02-05 | 2012-06-21 | Eppendorf Ag | Filter pipette tip |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO1999000193A1 (en) * | 1997-06-30 | 1999-01-07 | Rainin Instrument Co., Inc. | Improved membrane filter pipette tip |
| CN2566257Y (en) * | 2002-09-09 | 2003-08-13 | 黄一平 | Anti-pollution suction head for micro liquid suction device |
| US20120156114A1 (en) * | 2005-02-05 | 2012-06-21 | Eppendorf Ag | Filter pipette tip |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110785228A (en) * | 2018-10-29 | 2020-02-11 | 深圳逗点生物技术有限公司 | Porous modified adsorbent for solid phase extraction, preparation method thereof and solid phase extraction device |
| WO2020087209A1 (en) * | 2018-10-29 | 2020-05-07 | 深圳逗点生物技术有限公司 | Porous modified adsorbent for solid-phase extraction, preparation method therefor and solid-phase extraction device |
| CN110812942A (en) * | 2019-12-20 | 2020-02-21 | 严润达 | Sewage trickling treatment device with disc-shaped filter element |
| CN112827523A (en) * | 2021-01-08 | 2021-05-25 | 上海金鑫生物科技有限公司 | Ultrahigh molecular polyethylene filter containing activated carbon |
| CN115304848A (en) * | 2022-08-19 | 2022-11-08 | 智滤(苏州)材料科技有限公司 | Formula and manufacturing process of color-developing water-blocking filter element |
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Application publication date: 20141210 |