CN104171791A - Preparation method of solid honey with antioxidant and anticancer effects and product thereof - Google Patents

Abstract

The invention discloses a preparation method of solid honey with anti-oxidation and anti-cancer effects and its products. During preparation, honey from different sources is prepared and mixed, and then preheated at 15°C-35°C, and the honey is preheated per kilogram. Weigh 20-100 μg of selenium-VE, and then add it to preheated honey equivalent to 5-9 times the weight of selenium-VE, stir after adding to make the temperature of honey reach 45 ° C ~ 60 ° C, and then carry out ultrasonic emulsification by equal volume addition method, vacuum Suction filtration, after suction filtration, freeze the mixture at -20℃～-25℃ for 12～48h, then store it under the condition of vacuum degree of -0.6MPa～-1.0MPa for 0.5～1.5h, and then store it at 30～ Heating under vacuum conditions at 40°C until the water content in the mixture is 1%-3% by weight; cooling and pulverizing, the method is simple, can guarantee the original flavor and quality of honey, and can improve the anti-oxidation and anti-cancer effects enhanced.

Description

Preparation method and product of solid honey with antioxidant and anticancer effects

technical field

The invention belongs to the field of health product processing, and in particular relates to a preparation method of solid honey with antioxidant and anticancer effects and a product prepared by the method.

Background technique

With the gradual improvement of people's living standards, people pay more and more attention to health care and beauty. Therefore research a kind of food that has beautifying and anti-cancer, health-care effect, realize the purpose that edible food can reach beautifying and anti-cancer in people's life, realize the same source of medicine and food, is the direction of current development.

Honey is a translucent, shiny, thick liquid, white to light yellow or orange to yellowish brown, white granular crystals will gradually precipitate out after a long time or when it is cold. The main components of honey are fructose and glucose, the total content of the two is about 70%; it still contains a small amount of sucrose (sometimes the content is quite high), maltose, dextrin, gum, and nitrogenous compounds, organic acids, volatile oils, pigments, waxes, plant Fragments (especially pollen grains), yeast, enzymes, inorganic salts, etc.; but generally only contain trace vitamins, such as vitamin A, vitamin C, vitamin D, choline, vitamin B2, niacin, pantothenic acid, biotin, folic acid, B vitamins and vitamin K etc. Honey is widely loved as a health food due to its rich nutritional content. However, the disadvantage of honey is that it is fluid, and it is inconvenient to carry. For the convenience of carrying, honey is processed into solid honey. The main method is to add appropriate amount of auxiliary materials to process honey into powder, granule and block. However, most of the production engineering requires processing at a temperature higher than 100°C, which will destroy the active ingredients of honey and affect the original flavor and efficacy of honey.

Vitamin E has the function of scavenging free radicals, which can eliminate free radicals produced by sun exposure, pollution and stress, and protect skin tissue. It can take care of the root of the skin, so that the skin will not appear fine lines and sagging early. It can also promote skin microvascular circulation, keep the blood in the skin bright and clean forever, and make the face look naturally rosy and energetic. Selenium is an important component of some antioxidant enzymes (glutathione peroxidase) and selenium-P protein in the human body. It plays a role in balancing the redox atmosphere in the body and has anti-cancer effects. But, do not see the report that vitamin E (VE) and selenium and honey are made health food at present.

Contents of the invention

In view of this, one of the objectives of the present invention is to provide a method for preparing solid honey with antioxidant and anticancer effects; the second objective of the present invention is to provide a product prepared by the above method.

In order to realize the foregoing invention object, the present invention provides following technical scheme:

1. A method for preparing solid honey with antioxidant and anticancer effects, comprising the steps of:

(1) Blend and mix honey from different sources, and then preheat the honey at 15°C-35°C to obtain preheated honey;

(2) Weigh 20-100 μg of selenium-VE per kilogram of preheated honey, then add selenium-VE into preheated honey equivalent to 5-9 times the weight of selenium-VE, stir after adding to make the temperature of honey reach 45°C-60°C °C to obtain a premixture; the selenium-VE is a mixture of selenium and VE with a mass ratio of 1:1 to 5;

(3) Ultrasonic emulsification of the pre-mixture obtained in step (2), then add equal weight of preheated honey, continue ultrasonic emulsification, then add equal weight of preheated honey, continue ultrasonic emulsification, and repeat until step (1) The ultrasonic emulsification of the preheated honey is completed; the condition of the ultrasonic emulsification is under the condition of 15°C-60°C, the ultrasonic emulsification treatment with a frequency of 30-45kHz and a voltage of 30-40V is carried out for 20-30 minutes;

(4) vacuum filtration the mixture after ultrasonic emulsification in step (3) to make the particle size of honey reach 80～200 mesh;

(5) Freeze the suction-filtered mixture in step (4) at -20°C to -25°C for 12 to 48 hours, then move it into a vacuum dryer, and freeze it at -20°C to -25°C with a vacuum of - Store under the condition of 0.6MPa～-1.0MPa for 0.5～1.5 hours, then heat at 30～40°C and vacuum degree of -0.6MPa～-1.0MPa until the weight percentage of water in the mixture is 1%-3%;

(6) Pulverize the mixture treated in step (5) at 20-25°C.

Preferably, in the step (1), the preheating is to preheat the honey at 30°C.

Preferably, the step (2) is to weigh 100 μg of selenium-VE per kilogram of preheated honey, then add selenium-VE to preheated honey equivalent to 9 times the weight of selenium-VE, and stir after adding to make the temperature of the honey reach 45 °C to obtain a premix.

More preferably, in the step (2), the stirring is performed at a speed of 2000 rpm for 10-20 minutes.

More preferably, in the step (3), the ultrasonic emulsification condition is 25 minutes at 15°C with a frequency of 45kHz and a voltage of 40V.

More preferably, the step (4) is to vacuum filter the ultrasonically emulsified mixture in the step (3) to make the particle size of the honey reach 200 mesh. 100 mesh for the second level and 200 mesh for the third level.

Preferably, the step (5) is to first freeze the suction-filtered mixture in the step (4) at -25°C for 48 hours, then move it into a vacuum dryer, and freeze it at -25°C with a vacuum of -0.8MPa. It was stored under the condition for 1.5 hours, and thereafter heated at 35° C. and a vacuum of -0.8 MPa until the weight percentage of the water in the mixture was 2%.

2. The solid honey with anti-oxidation and anti-cancer properties prepared by the preparation method.

The beneficial effects of the present invention are: the present invention discloses a preparation method of solid honey with anti-oxidation and anti-cancer effects. The preparation process of the method is simple, the processing time is short, and the processing temperature is controlled below 60°C, which can ensure the original properties of the honey. Flavor and quality, solid honey has good solubility, and conventional honey can be obtained by adding an appropriate amount of water, and the present invention adds selenium and vitamin E to solid honey at the same time, so that the anti-oxidation and anti-cancer effects are enhanced, and at the same time It can also invigorate the spleen and calm the nerves, invigorate qi and blood.

Detailed ways

The present invention will be described in detail below in conjunction with preferred embodiments. For the experimental methods that do not specify specific conditions in the examples, usually follow the conventional conditions or the conditions suggested by the manufacturer.

Example 1

The preparation method of the solid honey with antioxidant and anticancer effects comprises the following steps:

(1) Blend and mix honey from different sources, and then preheat the honey at 30°C to obtain preheated honey; preheating ensures the fluidity of the honey and facilitates subsequent processing;

(2) Weigh 60 μg of selenium-VE per kilogram of preheated honey, then add selenium-VE to preheated honey equivalent to 5 times the weight of selenium-VE, and stir with a hand-held mixer at a speed of 2000 rpm after adding After 15 minutes, the temperature of the honey reached 50°C to obtain a premixture; the selenium-VE was a mixture of selenium and VE with a mass ratio of 1:3;

(3) Ultrasonic emulsification of the pre-mixture obtained in step (2), then add equal weight of preheated honey, continue ultrasonic emulsification, then add equal weight of preheated honey, continue ultrasonic emulsification, and repeat until step (1) The ultrasonic emulsification of the preheated honey is completed; the condition of the ultrasonic emulsification is to use ultrasonic emulsification with a frequency of 30kHz and a voltage of 30V for 20 minutes at 30°C;

(4) vacuum filter the mixture after ultrasonic emulsification in step (3) to make the particle size of honey reach 80 orders;

(5) Freeze the suction-filtered mixture in step (4) at -20°C for 12 hours, then move it into a vacuum dryer, and store it at -20°C with a vacuum of -0.6MPa for 0.5 hours. Thereafter, heating at 30°C and a vacuum of -0.6MPa for 0.5 hours until the weight percentage of water in the mixture is 3%;

(6) Pulverizing the mixture treated in step (5) at room temperature (20° C.) to obtain solid honey with antioxidant and anticancer effects.

Randomly extract 1g of the prepared solid honey with anti-oxidation and anti-cancer effects, and then according to "GB8254-1987: Determination of Food Grade Sodium Selenite in National Standard of the People's Republic of China and GB12388-1990: National Standard of the People's Republic of China Food The test results show that per kilogram of solid honey product contains 15 μg of selenium and 45 μg of VE, which meet the product requirements, and there is no peculiar smell after processing.

Example 2

The preparation method of the solid honey with antioxidant and anticancer effects comprises the following steps:

(1) Blend and mix honey from different sources, and then preheat the honey at 35°C to obtain preheated honey; preheating ensures the fluidity of the honey and facilitates subsequent processing;

(2) Weigh 100 μg of selenium-VE per kilogram of preheated honey, then add selenium-VE to preheated honey equivalent to 7 times the weight of selenium-VE, and stir with a hand-held mixer at a speed of 2000 rpm after adding After 20 minutes, the temperature of honey reached 60°C to obtain a premixture; the selenium-VE was a mixture of selenium and VE with a mass ratio of 1:5;

(3) Ultrasonic emulsification of the pre-mixture obtained in step (2), then add equal weight of preheated honey, continue ultrasonic emulsification, then add equal weight of preheated honey, continue ultrasonic emulsification, and repeat until step (1) The ultrasonic emulsification of the preheated honey is completed; the condition of the ultrasonic emulsification is to use ultrasonic emulsification with a frequency of 40kHz and a voltage of 40V for 30 minutes at 60°C;

(4) vacuum filter the mixture after ultrasonic emulsification in step (3) to make the particle size of honey reach 120 mesh; adopt 3-stage filtration mode during suction filtration, the first stage is 80 mesh, the second stage is 100 mesh, and the third stage is 80 mesh. Grade 120 mesh;

(5) Freeze the suction-filtered mixture in step (4) at -25°C for 24 hours, then transfer it to a vacuum dryer, freeze and store at -25°C with a vacuum of -1.0MPa for 1 hour , then heated at 40°C and a vacuum of -1.0MPa for 1 hour until the weight percentage of water in the mixture was 1%;

(6) Pulverizing the mixture treated in step (5) at room temperature (22° C.) to obtain solid honey with antioxidant and anticancer effects.

Randomly extract 1g of the prepared solid honey with anti-oxidation and anti-cancer effects, and then according to "GB8254-1987: Determination of Food Grade Sodium Selenite in National Standard of the People's Republic of China and GB12388-1990: National Standard of the People's Republic of China Food The test results show that per kilogram of solid honey product contains 16.76 μg of selenium and 83.24 μg of VE, which meet the product requirements, and there is no peculiar smell after processing.

Example 3

The preparation method of the solid honey with antioxidant and anticancer effects comprises the following steps:

(1) Blend and mix honey from different sources, and then preheat the honey at 15°C to obtain preheated honey; preheating ensures the fluidity of the honey and facilitates subsequent processing;

(2) Weigh 20 μg of selenium-VE per kilogram of preheated honey, then add selenium-VE to preheated honey equivalent to 9 times the weight of selenium-VE, and stir with a hand-held mixer at a speed of 2000 rpm after adding After 10 minutes, the temperature of the honey reached 45°C to obtain a premixture; the selenium-VE was a mixture of selenium and VE with a mass ratio of 1:1;

(3) Ultrasonic emulsification of the pre-mixture obtained in step (2), then add equal weight of preheated honey, continue ultrasonic emulsification, then add equal weight of preheated honey, continue ultrasonic emulsification, and repeat until step (1) The ultrasonic emulsification of the preheated honey is completed; the condition of the ultrasonic emulsification is to use ultrasonic emulsification with a frequency of 45kHz and a voltage of 40V for 25 minutes at 15°C;

(4) vacuum filter the mixture after ultrasonic emulsification in step (3) to make the particle size of honey reach 200 mesh; adopt 3-stage filtration mode during suction filtration, the first stage is 80 mesh, the second stage is 100 mesh, and the third stage is 100 mesh. Grade 200 mesh;

(5) Freeze the suction-filtered mixture in step (4) at -25°C for 48 hours, then move it into a vacuum dryer, and store it at -25°C with a vacuum of -0.8MPa for 1.5 hours. Thereafter, heating at 35°C and a vacuum of -0.8MPa for 1.5 hours until the weight percentage of water in the mixture is 2%;

(6) Pulverizing the mixture treated in step (5) at room temperature (22° C.) to obtain solid honey with antioxidant and anticancer effects.

Randomly extract 1g of the prepared solid honey with anti-oxidation and anti-cancer effects, and then according to "GB8254-1987: Determination of Food Grade Sodium Selenite in National Standard of the People's Republic of China and GB12388-1990: National Standard of the People's Republic of China Food The test results show that each kilogram of solid honey product contains 10 μg of selenium and 10 μg of VE, which meet the product requirements, and there is no peculiar smell after processing.

comparative example

Prepare solid honey according to the method of Example 1, the difference is that selenium-VE is not added in the preparation process.

Application example

Adopt 55 healthy primary weaned Wistar rats, weighing 65-85 grams, half male and half male, randomly divided into five groups according to body weight and sex, 12-14 rats in each group, respectively low-dose group, middle-dose group and high-dose group Group and Control 1 and Control 2. Wherein the low-dose group, the middle-dose group, and the high-dose group are respectively fed the mice with the selenium-VE amount of 20 μg/kg, 60 μg/kg, and 100 μg/kg; Group 2 was fed with 100 μg/kg selenium-VE

The experimental period was 8 weeks, and the feed and drinking water were not limited.

After the experiment, blood was taken from the abdominal aorta, and the plasma was separated to obtain the plasma, and then the activity of SOD and GSH-Px in the plasma was measured. The results are shown in Table 1.

Table 1. Effect of solid honey on SOD and GSH-Px

group SOD activity (U/ml) GSH-Px activity (U/ml) Control group 1 132.01±16.43 105.21±21.35 Control group 2 144.02±19.42 126.22±22.32 low dose group 164.29±24.66 126.21±41.71 Middle dose group 188.17±10.69* 131.56±22.33* high dose group 189.79±10.62* 139.65±37.82*

* means there is a significant difference compared with the control group (P﹤0.05).

It can be seen from Table 1 that feeding solid honey containing selenium-VE can increase the activity of SOD and GSH-Px, indicating that solid honey with selenium-VE has a significant antioxidant effect, so it has an antioxidant effect.

Gastric epithelial cancer cells in the logarithmic phase were cultured, and solid honey with different concentrations of selenium-VE was added to co-cultivate the cells. The control group did not add sodium selenite. The culture was terminated at different times, the cells were collected, fixed with paraformaldehyde for 30 minutes, washed 3 times with PBS buffer for 5 minutes each, and pre-cooled for 5 minutes. In situ detection of apoptosis was performed according to the instructions of the apoptosis in situ detection kit. The apoptotic cells were observed with an optical microscope and the apoptotic index was calculated. After staining with TUNEL kit, the apoptotic cells appeared blue-black or black particles in the nucleus.

Apoptotic index calculation method: count 5 high-power microscopes greater than 1000 cells, and count the number of apoptotic cells and total cells respectively. Apoptosis index=number of apoptotic cells/number of total cells*100%, the results are shown in Table 2.

Table 2. The effect of solid honey on the apoptosis index of gastric epithelial cancer cells

* means there is a significant difference compared with the control group (P<0.05).

It can be known from Table 2 that feeding solid honey containing selenium-VE can significantly increase the apoptosis index of gastric epithelial cancer cells, indicating that the solid honey prepared by the present invention has a significant anticancer effect.

Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that it can be described in terms of form and Various changes may be made in the details without departing from the scope of the invention defined by the claims.

Claims (8)

1. the preparation method with the solid bee honey of anti-oxidant and anticancer function, is characterized in that, comprises the steps:

(1) by the different honey blending and mixing in source, then, by honey preheating under 15 ℃ of-35 ℃ of conditions, obtain preheating honey;

(2) by every kilogram of preheating honey, take selenium-VE20-100 μ g, then selenium-VE is added and be equivalent to, in selenium-VE weight 5-9 preheating honey doubly, add rear stirring to make honey temperature reach 45 ℃～60 ℃, obtain premix; Described selenium-VE is the mixture that selenium and VE mass ratio are 1:1～5;

(3) step (2) gained premix is carried out to ultrasonic emulsification, the preheating honey that the weight such as then adds, continue ultrasonic emulsification, and then the preheating honey of weight such as add, continue ultrasonic emulsification, so repeatedly until step (1) gained preheating honey ultrasonic emulsification is complete; The condition of described ultrasonic emulsification is that proportion is 30～45kHz under 15 ℃～60 ℃ conditions, and the ultrasonic emulsification that voltage is 30～40V is processed 20～30 minutes;

(4) by step (3), the mixture after ultrasonic emulsification makes the granularity of honey reach 80～200 orders with vacuum filtration;

(5) by the mixture of step (4) suction filtration first under the condition of-20 ℃～-25 ℃ freezing 12～48 hours, then move in vacuum drier, under the condition that is-0.6MPa～-1.0MPa at-20 ℃～-25 ℃, vacuum, preserve 0.5～1.5 hour, the percentage by weight that is after this moisture content in being heated to mixture under-0.6MPa～-1.0MPa condition at 30～40 ℃, vacuum is 1%-3%;

(6) mixture of processing through step (5) is pulverized and be get final product under 20-25 ℃ of condition.

2. preparation method according to claim 1, is characterized in that: in described step (1), described preheating is by honey preheating under 30 ℃ of conditions.

3. according to the preparation method described in claim 1-2 any one, it is characterized in that: described step (2) is for to take selenium-VE100 μ g by every kilogram of preheating honey, then selenium-VE is added in the preheating honey that is equivalent to 9 times of selenium-VE weight, add rear stirring to make honey temperature reach 45 ℃, obtain premix.

4. preparation method according to claim 3, is characterized in that: in described step (2), described stirring is the rotating speed stirring 10-20 minute with 2000 revs/min.

5. preparation method according to claim 1, is characterized in that: in described step (3), the condition of described ultrasonic emulsification is under 15 ℃ of conditions, and proportion is 45kHz, and the ultrasonic emulsification that voltage is 40V is processed 25 minutes.

6. preparation method according to claim 1, it is characterized in that: described step (4) is, mixture by step (3) after ultrasonic emulsification makes the granularity of honey reach 200 orders with vacuum filtration, described suction filtration adopts the filter type of 3 grades, the first order 80 orders, the second level 100 orders, the third level 200 orders.

7. preparation method according to claim 1, it is characterized in that: described step (5) is by the mixture of step (4) suction filtration first under the condition of-25 ℃ freezing 48 hours, then move in vacuum drier, under the condition that is-0.8MPa at-25 ℃, vacuum, preserve 1.5 hours, the percentage by weight that is after this moisture content in being heated to mixture under-0.8MPa condition at 35 ℃, vacuum is 2%.

8. what by preparation method described in claim 1-7 any one, made has an anti-oxidant and anticancer solid bee honey.