There is Actinidia arguta Sieb.et Zucc leaf tea of antioxidation, function of reducing blood sugar and preparation method thereof
Technical field
The present invention discloses and a kind of relates to Actinidia arguta Sieb.et Zucc leaf tea with antioxidation, function of reducing blood sugar and preparation method thereof, belongs to Tea Production preparing technical field.
Background technology
Actinidia arguta Sieb.et Zucc leaf be Actinidiaceae actinidia Actinidia arguta Sieb.et Zucc [Actinidia arguta (Sieb. et
Zucc.) Planch. ex Miquel.] tender leaf.Actinidia arguta Sieb.et Zucc leaf contains ursolic acid, oleanolic acid, succinic acid, daucosterol, vitamin C, aminoacid, trace element and flavones ingredient, has fat-eliminating slimming, effect of anti-ageing anti-cancer, because being in the development phase, essentially wild state.
As the resource that a kind of healthy nutritive value is the highest, the most do not develop easy to carry in China at present, it is easy to take and have the product of effect.Actinidia arguta Sieb.et Zucc leaf tea will have wide market prospect as a kind of beverage easily accepted by broad masses of the people.
Summary of the invention:
The present invention discloses a kind of Actinidia arguta Sieb.et Zucc leaf tea with antioxidation, effect of lowering blood sugar, it is provided that a kind of new Actinidia arguta Sieb.et Zucc leaf is easy to carry about with one, instant, easy absorption of human body.
The present invention discloses a kind of Actinidia arguta Sieb.et Zucc leaf tea preparation method with antioxidation, effect of lowering blood sugar, maintains Folium Schisandrae Chinensis color and nutritive and health protection components is not destroyed.
A kind of Actinidia arguta Sieb.et Zucc leaf tea preparation method with antioxidation, effect of lowering blood sugar of disclosure of the invention, it is characterised in that comprise the following steps that:
1) select: Actinidia arguta Sieb.et Zucc leaf is removed the foreign material such as old stalk;
2) quick-freezing: the Actinidia arguta Sieb.et Zucc leaf of select is put into freezer sharp freezing room (18~20 DEG C), quick-freezing 0.5 hour, then moves into cold preservation storehouse and preserves;
3) spread: Actinidia arguta Sieb.et Zucc is frozen leaf and naturally spreads on bamboo plaque 6 hours, spread thickness about 2-2.5cm;
4) complete: use roller fixation machine, start to feed intake during temperature about 220~230 DEG C in cylinder, rolling several times, to Actinidia arguta Sieb.et Zucc freeze that leaf is crisp, obstruct soft folding and constantly time stop;
5) moisture regain: the leaf drying in the air cool after completing loads sealing in sealing bucket and gets damp again to leaf deliquescing;
6) knead: use kneading machine, the raw material after moisture regain is put into kneading machine, kneads with principle light, heavy, light;Pressurization in 5 minutes once, is obstructed flat for terminal, and stopping in 3~5 minutes is rubbed in decompression again;
7) deblock: break up by hand;
8) molding: starting dual-pot curved pekoe roasting machine, select small size shelves, temperature arranges 110 DEG C, puts into the raw material after deblocking and forms parch, treats that bottom goes out end, and leaf hands pinches frangible stopping, taking out;
9) it is dried: using tea drier, interior temperature about 80 DEG C, to dry;
10) Titian: arrange 250 DEG C, in cylinder, temperature 130~140 DEG C of rollings are several times;
11) spreading for cooling: dried Actinidia arguta Sieb.et Zucc leaf tea is placed on bamboo plaque and naturally spreads 10min;
12) pack: the Actinidia arguta Sieb.et Zucc leaf tea after spreading for cooling is taken out a part of quality inspection at random, other load sealing in aluminium foil bag and preserve.
A kind of Actinidia arguta Sieb.et Zucc leaf tea preparation method with antioxidation, effect of lowering blood sugar of the present invention, it is characterised in that:
Actinidia arguta Sieb.et Zucc leaf after step 1) being selected spreads process of withering in the sun, spreads thickness about 2-2.5cm, dry in the sun to obstruct flat go out wrinkle, be followed by step 3).
A kind of Actinidia arguta Sieb.et Zucc leaf tea preparation method with antioxidation, effect of lowering blood sugar of the present invention, it is characterised in that:
Raw material meat grinder after step 6) being kneaded rubs;The raw material of rubbing is put in the container meeting hygienic requirements, temperature of suitably heating in making stockpile at about 28 DEG C, aerobic fermentation about 3 hours, middle turning twice, take some fermentation raw materials and put into clear water to be observed when raw material is sepia and stop, be followed by step 7).
The positive effect of the present invention is: based on tradition tea-manufacturing technology; in conjunction with Actinidia arguta Sieb.et Zucc leaf feature; Actinidia arguta Sieb.et Zucc tender leaf machining limit is extended by technological means such as quick-freezings; inhibitory enzyme activity; tea polyphenols can be protected; solving Actinidia arguta Sieb.et Zucc tender leaf not concentrate collection period, long-time placement can make tea polyphenols isoreactivity composition problem of oxidation;Also make leaf cell film destroy simultaneously, be conducive to next step to distribute the blue or green stink of Actinidia arguta Sieb.et Zucc leaf.Maintain Actinidia arguta Sieb.et Zucc leaf color to greatest extent and nutritive and health protection components is not destroyed, it is easy to carry and take.Actinidia arguta Sieb.et Zucc leaf is used, makes and be easy to carry, preserve, it is easy to accepted and have the tea product of antioxidation, effect of lowering blood sugar by consumers in general.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention will be further described:
Embodiment
1
:
(1) select: remove Actinidia arguta Sieb.et Zucc tender leaf by hand and always obstruct and foreign material etc..
(2) quick-freezing: the Actinidia arguta Sieb.et Zucc leaf of select is put into freezer sharp freezing room (18~20 DEG C), quick-freezing 0.5 hour, then moves into cold preservation storehouse and preserves.
(3) spread: the Actinidia arguta Sieb.et Zucc that freezer takes out is frozen leaf and naturally spreads on bamboo plaque 6 hours, spread thickness about 2-2.5cm.
(4) completing: use roller fixation machine, start to feed intake in cylinder during temperature about 220~230 DEG C, rolling is several times, crisp to leaf, obstruct soft folding and constantly time stop.
(5) moisture regain: the leaf drying in the air cool after completing loads sealing in sealing bucket and gets damp again to leaf deliquescing.
(6) knead: use kneading machine, the raw material after moisture regain is put into kneading machine, kneads with principle light, heavy, light.Pressurization in 5 minutes once, is obstructed flat for terminal, and stopping in 3~5 minutes is rubbed in decompression again.
(7) deblock: manual.
(8) molding: starting dual-pot curved pekoe roasting machine, select small size shelves, temperature arranges 110 DEG C, suitably regulates the speed, puts into the raw material after deblocking and forms parch, treats that bottom goes out end, and leaf hands pinches frangible stopping, taking out.
(9) moisture regain: the raw material loading after shaping seals in bucket and naturally gets damp again 0.5 hour.
(10) it is dried: using tea drier, interior temperature about 80 DEG C, to dry.
(11) Titian: arrange 250 DEG C, in cylinder, temperature 130~140 DEG C of rollings are several times.
(12) spreading for cooling: dried Actinidia arguta Sieb.et Zucc leaf green tea is placed on bamboo plaque and naturally spreads 10min.
(13) pack: the Actinidia arguta Sieb.et Zucc leaf green tea after spreading for cooling is taken out a part of quality inspection at random, other load sealing in aluminium foil bag and preserve.
Antioxidant effect is tested:
Accurately weighing leaf tea 1.00g that embodiment 1 obtains, put into beaker, add 200mL, 95 DEG C of distilled water, insulation is placed 10 minutes, filters, and filtrate is standby.Accurately weigh DPPH3.9mg, after adding a small amount of anhydrous alcohol solution, be settled to 100mL with the ethanol solution that volume fraction is 95%, make the DPPH solution that concentration is 0.2mmol/L.Take the DPPH solution that 2mL has prepared, add 1mL leaf tea filtrate, supply 4mL with 95% ethanol, mixing, after placing 30min, at 517nm, measure sample light absorption value As, with 95% ethanol as blank, measure Ac.Computing formula is: clearance rate/%=(Ac-As)/Ac × 100.After measured, calculating, the clearance rate of filtrate is 78.6%.
Embodiment
2
:
(1) select: remove Actinidia arguta Sieb.et Zucc tender leaf by hand and always obstruct and foreign material etc..
(2) spread: the Actinidia arguta Sieb.et Zucc leaf after selecting spreads 6 hours on bamboo plaque naturally, spread thickness about 2-2.5cm.
(3) completing: use roller fixation machine, start to feed intake in cylinder during temperature about 220~230 DEG C, rolling is several times, crisp to leaf, obstruct soft folding and constantly time stop.Moisture about 50% after completing.
(4) moisture regain: the leaf drying in the air cool after completing loads sealing in sealing bucket and gets damp again to leaf deliquescing.
(5) knead: use kneading machine, the raw material after moisture regain is put into kneading machine, kneads with principle light, heavy, light.Pressurization in 5 minutes once, is obstructed flat for terminal, and stopping in 3~5 minutes is rubbed in decompression again.
(6) deblock: manual.
(7) molding: starting dual-pot curved pekoe roasting machine, select small size shelves, temperature arranges 110 DEG C, suitably regulates the speed, puts into the raw material after deblocking and forms parch, treats that bottom goes out end, and leaf hands pinches frangible stopping, taking out.
(8) moisture regain: the raw material loading after shaping seals in bucket and naturally gets damp again 0.5 hour.
(9) it is dried: using tea drier, interior temperature about 80 DEG C, to dry.
(10) Titian: arrange 250 DEG C, in cylinder, temperature 130~140 DEG C of rollings are several times.
(11) spreading for cooling: the Actinidia arguta Sieb.et Zucc leaf green tea after Titian is placed on bamboo plaque and naturally spreads 10min.
(12) pack: the Actinidia arguta Sieb.et Zucc leaf green tea after spreading for cooling is taken out a part of quality inspection at random, other load sealing in aluminium foil bag and preserve.
Antioxidant effect is tested:
Accurately weighing leaf tea 1.00g that embodiment 2 obtains, put into beaker, add 200mL, 95 DEG C of distilled water, insulation is placed 10 minutes, filters, and filtrate is standby.Accurately weigh DPPH3.9mg, after adding a small amount of anhydrous alcohol solution, be settled to 100mL with the ethanol solution that volume fraction is 95%, make the DPPH solution that concentration is 0.2mmol/L.Take the DPPH solution that 2mL has prepared, add 1mL leaf tea filtrate, supply 4mL with 95% ethanol, mixing, after placing 30min, at 517nm, measure sample light absorption value As, with 95% ethanol as blank, measure Ac.Computing formula is: clearance rate/%=(Ac-As)/Ac × 100.After measured, calculating, the clearance rate of filtrate is 44.7%.
Embodiment
3
:
(1) select: the manual Actinidia arguta Sieb.et Zucc leaf (great Ye, Lao Ye) that removes obstructs and foreign material etc. always.
(2) wither: raw material being spread in the sun (sunlight is not too strong, shelters from heat or light if can put up a shed the most by force), spreading thickness about 2-2.5cm, dry in the sun to obstruct flat go out wrinkle.
(3) knead: kneading machine put into by the raw material after withering, knead with principle light, heavy, light.Pressurization in 5 minutes once, is obstructed flat for terminal, and stopping in 3~5 minutes is rubbed in decompression again.
(4) broken: the raw material meat grinder after kneading rubs.
(5) fermentation: the raw material of rubbing is put in the container meeting hygienic requirements, temperature of suitably heating in making stockpile at about 28 DEG C, aerobic fermentation about 3 hours, middle turning twice, take some fermentation raw materials and put into clear water to be observed when raw material is sepia and stop.
(6) being dried: roller fixation machine arranges 140 DEG C, in cylinder, temperature less than 100 DEG C will be fermented raw material stoving completely.
(7) Titian: arrange temperature 190~200 DEG C, the raw material after drying slowly puts in cylinder, and in application cylinder, temperature 140 DEG C is rolled several times.
(8) spreading for cooling: the Actinidia arguta Sieb.et Zucc leaf broken black tea after Titian is placed in sun-drying tray and naturally spreads 10min.
(9) pack: the Actinidia arguta Sieb.et Zucc leaf broken black tea after spreading for cooling is taken out a part of quality inspection at random, other load sealing in aluminium foil bag and preserve.
Antioxidant effect is tested:
Accurately weighing leaf tea 0.50g that embodiment 3 obtains, put into beaker, add 200mL, 95 DEG C of distilled water, insulation is placed 10 minutes, filters, and filtrate is standby.Accurately weigh DPPH3.9mg, after adding a small amount of anhydrous alcohol solution, be settled to 100mL with the ethanol solution that volume fraction is 95%, make the DPPH solution that concentration is 0.2mmol/L.Take the DPPH solution that 2mL has prepared, add 1mL leaf tea filtrate, supply 4mL with 95% ethanol, mixing, after placing 30min, at 517nm, measure sample light absorption value As, with 95% ethanol as blank, measure Ac.Computing formula is: clearance rate/%=(Ac-As)/Ac × 100.After measured, calculating, the clearance rate of filtrate is 41.2%.
Actinidia arguta Sieb.et Zucc leaf tea hypoglycemic effect of the present invention is tested:
Prepared by sample: accurately weigh leaf tea (embodiment 1 sample and embodiment 2 sample respectively account for half) 10.0g, puts into beaker, adds 200mL, 90 DEG C of distilled water, and insulation is placed 15 minutes, filters.Again adding 200mL, 90 DEG C of distilled water in tea grounds, insulation is placed 15 minutes, filters.Twice filtrate merges, and concentrates, standby.
The foundation of alloxan diabetes mouse model: mice adaptability feeds 3d, during modeling, water 12h is can't help in mice fasting, disposable celiac injection alloxan 200mg/kg, (being made into mass fraction 2% solution with normal saline) ad lib afterwards, tail venous blood sampling after 72h, measure blood glucose value, take blood glucose value > 11.1mmol/L person for model successfully mice.
Experimental technique: modeling success mice is randomly divided into 5 groups, often group 10, i.e. being divided into dosage group (400mg/kg), Actinidia arguta Sieb.et Zucc leaf tea high dose group (800mg/kg) in hyperglycemia model group (gavage equivalent distilled water), positive drug control group (100mg/kg metformin hydrochloride), Actinidia arguta Sieb.et Zucc leaf tea low dose group (200mg/kg), Actinidia arguta Sieb.et Zucc leaf tea, separately taking 10 healthy mices is blank group (gavage equivalent distilled water).Each group press 10mL/kg and is administered, every day gavage once, continuous 28d, experiment terminates mensuration blood glucose.
Blood sugar detection uses glucose-peroxidase method, fasting 12 hours before blood sugar detection.
Experimental result: be shown in Table 1.
The impact on blood glucose in diabetic mice of the table 1 Actinidia arguta Sieb.et Zucc leaf tea
Note: the entirely different person of lower case is significant difference (P < 0.05);The entirely different person of capitalization is significant difference (P < 0.05).
Interpretation of result: Actinidia arguta Sieb.et Zucc leaf tea, the most middle and high dosage is notable on mouse blood sugar impact, has blood sugar reducing function, and hypoglycemic effect is proportionate with taking dose.Actinidia arguta Sieb.et Zucc leaf tea hypoglycemic effect is more weak compared with positive drug metformin hydrochloride, and difference is extremely notable, therefore cannot function as the drug use of replacement therapy diabetes, but can assistant hypoglycemic.