CN104163684B - A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin - Google Patents
A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin Download PDFInfo
- Publication number
- CN104163684B CN104163684B CN201410311197.0A CN201410311197A CN104163684B CN 104163684 B CN104163684 B CN 104163684B CN 201410311197 A CN201410311197 A CN 201410311197A CN 104163684 B CN104163684 B CN 104163684B
- Authority
- CN
- China
- Prior art keywords
- link
- militaris
- parts
- cordyccps
- shaddock skin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention discloses a kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin, is made up of the raw material of following weight part: nutritive medium 43��53 parts, major ingredient 38��48 parts, dried silkworm chrysalis meal 6��13 parts, shaddock skin 8��17 parts. Adopting the Cordyccps-militaris-(L.)-link. Sporophore culture medium culturing Cordyceps militaris (L.) Link. of the present invention, in Cordyccps-militaris-(L.)-link. Sporophore process of growth, it is possible to effectively suppress varied bacteria growing, protection bacterial classification, the Cordyceps militaris (L.) Link. resistance of oxidation simultaneously turned out strengthens.
Description
Technical field
The present invention relates to culture medium of edible fungus field, in particular to a kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin.
Background technology
Cordyceps militaris (L.) Link., also known as Cordyceps militaris or Cordyceps militaris (L.) Link., belongs to Mycophyta, Ascomycotina, Hypocreales, Clavicipitaceae, Cordyceps, parasitic on the pupa bodies such as Noctuidae, is distributed in all over the world, be a kind of generally acknowledge both at home and abroad not only can eat but also pharmaceutically useful fungi. Cordyceps militaris (L.) Link. and Cordyceps sinensis belong to xenogenesis together, are China's the widest two kinds of Cordyceps with pharmaceutical use of distribution. Research shows that Cordyceps militaris (L.) Link. and Cordyceps sinensis have same alike result, and similar pharmacological function and detailed clinical effectiveness, alternative Cordyceps sinensis is used as medicine. Containing compositions such as cordycepin, Cordyceps polysaccharide, cordycepic acid, adenosines in Cordyceps militaris (L.) Link.; have and strengthen Tumor suppression immune, anti-oxidant, antiviral, antibacterial, obvious growth, prophylactic treatment cerebral thrombosis, Intracerebral hemorrhage, renal failure; diuresis, suppression Platelet congregation prevent thrombosis; elimination face spot; anti-ageing wrinkle resistant, cardioprotection, liver, the multiple function such as anti-convulsion.
Wild Chinese caterpillar fungus resource-constrained, therefore people start then the research artificial culture of Chinese caterpillar fungus. Cordyceps militaris (L.) Link. is relatively low for the requirement of growing environment; artificial culture is easier to by contrast; main with liquid fermenting formation mycelium at present, obtain sporophore by artificial extensive solid culture, carry out the production of mass-producing Cordyceps militaris (L.) Link. additionally by live body cultivation and become a reality. Cordyceps militaris (L.) Link., in culturing process, if sterilization is not good or sterilization is not tight, easily occurs bacterium, yeast and mould etc. to pollute. If there is bacterium, yeast and mould contamination, being further purified by master clock, serious will be eliminated. Bacterium, yeast and mould are to the pollution of Cordyceps militaris (L.) Link. as can be seen here, will cause and cultivate financial resources, material resources loss, and increase unnecessary trouble. Find a kind of new demand having the substratum of bacteria resistance function to become Chinese caterpillar fungus culture medium field.
Shaddock is the mature fruit of rutaceae shaddock, mainly originates in the southern areas such as China Fujian, Jiangxi, Guangdong, Guangxi, and all there is cultivation on the ground such as Hunan of present China, Zhejiang, Sichuan. Pomelo fragrant, sour-sweet, Liang Run, nutritious, pharmaceutical use is very high, is one of the famous and precious fruit of people's eating, is also the fruit of the most food therapy value that medical circle is generally acknowledged. The epidermis that shaddock skin is shaddock has another name called Exocarpium Citri Rubrum, warm in nature, bitter, pungent, has the effect of activating QI to eliminate phlegm, strengthening the spleen to promote digestion, loose cold eliminating dampness. And people are after having eaten shaddock, shaddock skin is often treated as rubbish and abandons, and is not only environment protection and brings burden, and the effect simultaneously also reducing shaddock entirety is worth. Containing a large amount of naringins in shaddock skin, it is belong to flavonoid compound also known as BIOFLAVONOLDS, it is a class low molecule natural plant composition, be some secondary metabolites that plant produces in long-term natural selection process. Much research shows, flavonoid compound have anti-oxidant, antianaphylaxis, antisepsis and anti-inflammation, anti-mutation, step-down, hypoglycemic, antitumor, delay senility and the effect such as liver-protecting and stomach-protecting. Also there are some researches show, shaddock skin contains the element of the needed by human such as very abundant protein, organic acid, VITAMIN and calcium, phosphorus, magnesium, sodium. Shaddock skin is applied in Cordyccps-militaris-(L.)-link. Sporophore substratum there is wide market outlook and economic worth.
Summary of the invention
Technical problem to be solved by this invention is to provide one and has bacteria resistance function, it is possible to strengthen the Cordyccps-militaris-(L.)-link. Sporophore substratum of the interpolation shaddock skin of Cordyceps militaris (L.) Link. anti-oxidation efficacy.
For achieving the above object, the present invention takes following technical scheme:
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for shaddock skin, it is made up of the raw material of following weight part: nutritive medium 43��53 parts, major ingredient 38��48 parts, dried silkworm chrysalis meal 6��13 parts, shaddock skin 8��17 parts.
The Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation shaddock skin of the present invention, it is characterised in that, it is made up of the raw material of following weight part: nutritive medium 46��51 parts, major ingredient 40��44 parts, dried silkworm chrysalis meal 9��11 parts, shaddock skin 11��14 parts.
Major ingredient of the present invention is one or more in rice, wheat, corn, soya bean, when multiple mixing, it is possible to be arbitrary proportion. Suitably add carbon source in the medium, such as rice, wheat, corn, soya bean, be conducive to Cordyceps militaris (L.) Link. synthetic carbohydrate and amino acid.
Nutritive medium of the present invention is made up of following raw material: sugar 0.7��1.7 part, yeast extract paste 0.7��1.4 part, potassium primary phosphate 0.02��0.04 part, 0.02��0.04 part, magnesium sulfate, ammonium citrate 0.02��0.04 part, VITMAIN B1 0.002��0.004 part, 96��99 parts, water. Generally meet Cordyceps militaris (L.) Link. to the demand of mineral element by adding inorganic salts, preferably sulfuric acid magnesium of the present invention, potassium primary phosphate, wherein potassium primary phosphate also has the pH value effect regulating substratum, substratum is adjusted to slant acidity, meets Cordyceps militaris (L.) Link. slant acidity demand. Owing to Cordyceps militaris (L.) Link. can not synthesize essential vitamin, suitably add VITMAIN B1 and be conducive to growing of Cordyceps militaris (L.) Link.. Suitably add nitrogen element inorganic salt in the medium, such as ammonium citrate, promote protein, the inorganic nitrogen such as organonitrogen and ammonium salt such as nucleic acid of Cordyceps militaris (L.) Link. self synthesis.
Sugar of the present invention is glucose or sucrose. Dextrose plus saccharoses etc. are small molecules carbohydrate, also as Cordyceps militaris (L.) Link. carbon source, promote that Cordyceps militaris (L.) Link. synthetic carbohydrate and amino acid whose effect are best.
The present invention adds the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin, and step is as follows:
(1) raw material pre-treatment: dry after pulverizing into powder by shaddock skin, crosses 50��100 order sieves, then major ingredient is pulverized into fine powder, crosses 100��200 order sieves; Take sugar, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, be heated to 90 DEG C��100 DEG C while stirring, continue stirring until raw material and all dissolve, be cooled to normal temperature, add VITMAIN B1, obtain nutritive medium;
(2) raw material mixing: major ingredient fine powder, shaddock peel powder end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs evenly, obtain mixed solution;
(3) bottle: being divided by mixed solution and be filled in culture vessel, Intake Quantity is the 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 30��45min under 119 DEG C��124 DEG C high temperature will be housed, be cooled to 25 DEG C��27 DEG C, obtains adding the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin.
Compared to existing technology, it is an advantage of the current invention that:
1, the flavones in shaddock skin and Flavonoid substances are to gram-positive cocci, such as staphylococcus, suis, pneumococcus, anthrax bacillus etc., and gram negative bacillus, as intestinal bacteria, dysentery bacterium, Corynebacterium diphtheriae, Bacillus proteus etc. have restraining effect. Cordyccps-militaris-(L.)-link. Sporophore substratum adds shaddock skin, effectively suppresses varied bacteria growing, protection Cordyceps militaris spawn.
2, the Flavonoid substances composition in shaddock skin has antioxygenation, and the present invention is by adding shaddock skin at Cordyccps-militaris-(L.)-link. Sporophore substratum, it is possible to strengthen Cordyceps militaris (L.) Link. anti-oxidation efficacy.
3, shaddock skin is rich in the element of the needed by human such as abundant protein, organic acid, VITAMIN and calcium, phosphorus, magnesium, sodium, as waste disposal, and waste resource, shaddock skin is added in Cordyccps-militaris-(L.)-link. Sporophore substratum, turn waste into wealth, make full use of resource, it is to increase the utility value of shaddock skin.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these embodiments.
Embodiment 1
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for shaddock skin, it is made up of the raw material of following weight part: nutritive medium 43 parts, 28 parts, rice, soya bean 10 parts, dried silkworm chrysalis meal 6 parts, shaddock skin 8 parts.
Nutritive medium, is made up of the raw material of following weight part: sucrose 0.7 part, yeast extract paste 0.7 part, potassium primary phosphate 0.02 part, 0.02 part, magnesium sulfate, ammonium citrate 0.02 part, VITMAIN B1 0.002 part, 96 parts, water.
The preparation of nutritive medium: take sucrose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 90 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to normal temperature, add VITMAIN B1, obtains nutritive medium.
Adding the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin, step is as follows:
(1) raw material pre-treatment: dry after pulverizing into powder by shaddock skin, crosses 50 order sieves, then rice, analysis for soybean powder is broken into fine powder, crosses 100 order sieves;
(2) raw material mixing: rice fine powder, soya bean fine powder, shaddock peel powder end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs evenly, obtain mixed solution;
(3) bottle: being divided by mixed solution and be filled in culture vessel, Intake Quantity is the 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 45min under 119 DEG C of high temperature will be housed, be cooled to 25 DEG C, obtains adding the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin.
Embodiment 2
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for shaddock skin, it is made up of the raw material of following weight part: nutritive medium 46 parts, 15 parts, rice, corn 15, soya bean 10, dried silkworm chrysalis meal 9 parts, shaddock skin 11 parts.
Nutritive medium, is made up of the raw material of following weight part: glucose 0.9 part, yeast extract paste 0.9 part, potassium primary phosphate 0.03 part, 0.03 part, magnesium sulfate, ammonium citrate 0.03 part, VITMAIN B1 0.003 part, 97 parts, water.
The preparation of nutritive medium: take glucose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 95 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to normal temperature, add VITMAIN B1, obtains nutritive medium.
Adding the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin, step is as follows:
(1) raw material pre-treatment: dry after pulverizing into powder by shaddock skin, crosses 80 order sieves, then rice, corn, analysis for soybean powder is broken into fine powder, crosses 150 order sieves;
(2) raw material mixing: rice fine powder, corn fine powder, soya bean fine powder, shaddock peel powder end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs evenly, obtain mixed solution;
(3) bottle: being divided by mixed solution and be filled in culture vessel, Intake Quantity is the 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 35min under 121 DEG C of high temperature will be housed, be cooled to 25 DEG C, obtains adding the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin.
Embodiment 3
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for shaddock skin, it is made up of the raw material of following weight part: nutritive medium 51 parts, wheat 30 parts, corn 14 parts, dried silkworm chrysalis meal 11 parts, shaddock skin 14 parts.
Nutritive medium, is made up of the raw material of following weight part: glucose 1.3 parts, yeast extract paste 1.1 parts, potassium primary phosphate 0.03 part, 0.03 part, magnesium sulfate, ammonium citrate 0.03 part, VITMAIN B1 0.003 part, 98 parts, water.
The preparation of nutritive medium: take glucose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 95 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to normal temperature, add VITMAIN B1, obtains nutritive medium.
Adding the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin, step is as follows:
(1) raw material pre-treatment: dry after pulverizing into powder by shaddock skin, crosses 100 order sieves, then wheat, Semen Maydis powder is broken into fine powder, crosses 200 order sieves;
(2) raw material mixing: wheat fine powder, corn fine powder, shaddock peel powder end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs evenly, obtain mixed solution;
(3) bottle: being divided by mixed solution and be filled in culture vessel, Intake Quantity is the 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 35min under 122 DEG C of high temperature will be housed, be cooled to 26 DEG C, obtains adding the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin.
Embodiment 4
Add a Cordyccps-militaris-(L.)-link. Sporophore substratum for shaddock skin, it is made up of the raw material of following weight part: nutritive medium 53 parts, wheat 28 parts, soya bean master 20 parts, dried silkworm chrysalis meal 13 parts, shaddock skin 17 parts.
Nutritive medium, is made up of the raw material of following weight part: sucrose 1.7 parts, yeast extract paste 1.4 parts, potassium primary phosphate 0.04 part, 0.04 part, magnesium sulfate, ammonium citrate 0.04 part, VITMAIN B1 0.004 part, 99 parts, water.
The preparation of nutritive medium: take sucrose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 100 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to normal temperature, add VITMAIN B1, obtains nutritive medium.
Adding the preparation of the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin, step is as follows:
(1) raw material pre-treatment: dry after pulverizing into powder by shaddock skin, crosses 80 order sieves, then wheat, analysis for soybean powder is broken into fine powder, crosses 150 order sieves;
(2) raw material mixing: wheat fine powder, soya bean fine powder, shaddock peel powder end, dried silkworm chrysalis meal are mixed, mixture is added in nutritive medium while stirring, stirs evenly, obtain mixed solution;
(3) bottle: being divided by mixed solution and be filled in culture vessel, Intake Quantity is the 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 30min under 124 DEG C of high temperature will be housed, be cooled to 27 DEG C, obtains adding the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin.
Comparative example
A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum, is made up of the raw material of following weight part: nutritive medium 46 parts, 15 parts, rice, corn 15, soya bean 10, wheat 11 parts, dried silkworm chrysalis meal 9 parts.
Nutritive medium, is made up of the raw material of following weight part: glucose 0.9 part, yeast extract paste 0.9 part, potassium primary phosphate 0.03 part, 0.03 part, magnesium sulfate, ammonium citrate 0.03 part, VITMAIN B1 0.003 part, 97 parts, water.
The preparation of nutritive medium: take glucose, yeast extract paste, dipotassium hydrogen phosphate, magnesium sulfate, ammonium citrate, add water, is heated to 95 DEG C while stirring, continues stirring until raw material and all dissolves, be cooled to normal temperature, add VITMAIN B1, obtains nutritive medium.
The preparation of Cordyccps-militaris-(L.)-link. Sporophore substratum, step is as follows:
(1) raw material pre-treatment: rice, corn, soya bean, wheat-flour are broken into fine powder, crosses 150 order sieves;
(2) raw material mixing: rice fine powder, corn fine powder, soya bean fine powder, wheat fine powder, dried silkworm chrysalis meal are mixed, added while stirring in nutritive medium by mixture, stirs evenly, obtains mixed solution;
(3) bottle: being divided by mixed solution and be filled in culture vessel, Intake Quantity is the 2/3 of culture vessel actual capacity;
(4) sterilization: mixed solution container sterilization 35min under 121 DEG C of high temperature will be housed, be cooled to 25 DEG C, obtain Cordyccps-militaris-(L.)-link. Sporophore substratum.
Test example 1: the present invention adds the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin to the restraining effect of miscellaneous bacteria
1, medium liquid preparation: the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4, comparative example are added water extraction by solid-liquid ratio 1:30 respectively, extract three times, united extraction liquid, concentrate to certain volume, add 3 times 95% alcohol settling, get supernatant concentration, drying, obtain embodiment 1, embodiment 2, embodiment 3, embodiment 4, comparative example substratum crude extract for subsequent use.
2, cultivate and qualification for examination bacterial classification and purifying thereof:
Staphylococcus, suis, anthrax bacillus, intestinal bacteria, Bacillus proteus, provided by Guangxi University's animal technical college.
Bacterial classification adopts continuous method of scoring to carry out purifying, and bacterium colony after cultivation adopts gram staining method to differentiate, the strain inclined plane after discriminating cultivates, for subsequent use.
Gram staining method: bacterium is first through basic dyestuff violet staining, and after iodine liquid mordant dyeing, decolours with alcohol, the bacterium purple having under certain condition is not divested, and what have is divested, and therefore bacterium can be divided into two big classes, the former is called gram-positive microorganism, and the latter is Gram-negative bacteria. For observing conveniently, after decolouring again with a kind of orchil as luxuriant in alkalescence red, dilute azaleine etc. and redye. Positive bacteria is band purple still, and negative bacterium is then by red-dyed. There are the bacillus of bud born of the same parents and most coccuses, and all actinomycetes and fungi are all gram positive reaction; The bactacin of the pathogenic property of vibrios, spirochete and great majority all presents negative reaction.
3, the preparation of bacteria culture medium:
Adopting beef-protein medium, in order to increase the speed of growth of bacterium, add 10g glucose to be improved by substratum in every lkg water, O.1Mpa sterilizing 20min, makes flat board.
4, for the preparation of examination bacterium liquid:
Choosing the bacterium colony of Purification and Characterization of learning from else's experience, be inoculated in broth medium, 36 DEG C of constant-temperature shaking culture are to muddy. Then draw a certain amount of bacterium liquid to be inoculated in several Boiling tubes, repeat above-mentioned CMC model. Bacterium liquid 10 times dilution, gets 0.1ml respectively and is applied to flat board, 36 DEG C of constant temperature culture, and bacterium colony counts after being formed, and makes 5 �� 1O8CFU/ml bacterium liquid.
5, punch tool method measures Cordyccps-militaris-(L.)-link. Sporophore substratum that the present invention adds shaddock skin to the restraining effect of miscellaneous bacteria:
Punching on flat board with the punch tool of R=0.6cm, then coating is own adds, through ready bacterium liquid, the solution that concentration is the different culture media crude extract for subsequent use of 100g/L in hole, and 37 DEG C of constant temperature culture were observed after 2��3 days, surveyed the size of its inhibition zone.
6, test-results:
Size statistics unit: the cm of the different Cordyccps-militaris-(L.)-link. Sporophore culture medium solution inhibition zone radius of table 1
| Bacillus proteus | Staphylococcus | Suis | Intestinal bacteria | Anthrax bacillus | |
| Embodiment 1 | 1.62 | 1.64 | 1.62 | 1.75 | 1.64 |
| Embodiment 2 | 1.64 | 1.65 | 1.67 | 1.76 | 1.66 |
| Embodiment 3 | 1.65 | 1.59 | 1.63 | 1.71 | 1.56 |
| Embodiment 4 | 1.57 | 1.57 | 1.60 | 1.69 | 1.59 |
| Comparative example | 0.33 | 0.23 | 0.34 | 0.36 | 0.31 |
As can be seen from Table 1, staphylococcus, suis, anthrax bacillus, intestinal bacteria, Bacillus proteus are had stronger restraining effect by the Cordyccps-militaris-(L.)-link. Sporophore substratum of the interpolation shaddock skin of the embodiment of the present invention 1, embodiment 2, embodiment 3, embodiment 4, and the Cordyccps-militaris-(L.)-link. Sporophore substratum not adding shaddock skin of comparative example is extremely faint to miscellaneous bacteria restraining effect.
Test example 2: the Cordyceps militaris (L.) Link. antioxygenation that the present invention cultivates
1, material: the Cordyceps militaris (L.) Link. that the embodiment of the present invention 2, embodiment 3, comparative example are cultivated.
2, test method:
(1) Cordyceps militaris (L.) Link. runic thing prepares extracting method: pulverized respectively by the Cordyceps militaris (L.) Link. that the embodiment of the present invention 2, embodiment 3, comparative example are cultivated, water extraction is added by solid-liquid ratio 1:35, extract three times, united extraction liquid, concentrate to certain volume, add 3 times 95% alcohol settling, get supernatant concentration, drying, obtain Cordyceps militaris (L.) Link. crude extract for subsequent use.
(2) mensuration of reducing power: sample is by the Tripotassium iron hexacyanide (K3Fe(CN)6) it is reduced into yellow prussiate of potash (K4Fe(CN)6), yellow prussiate of potash again with Fe3+Effect, generates ferriferro cyanide (Prussian blue), and to detect the size that Prussian blue absorbancy represents reducing power at 700nm wavelength place, absorbancy is more high, and the reducing power of sample is more strong, and oxidation-resistance is more strong. At 700nm wavelength place, measure the light absorption value that concentration is the Cordyceps militaris (L.) Link. crude extract that the embodiment of the present invention 2 of 5mg/ml, embodiment 3, comparative example are cultivated respectively.
(3) superoxide anion experiment is removed: at generation ultra-oxygen anion free radical (O2-) reaction system in, add the embodiment of the present invention 2 that concentration is 5mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 3, comparative example are cultivated, measure their clearance rates.
(4) experiment of hydroxyl radical free radical is removed: FeSO4With H2O2Reaction produces OH, adds Whitfield's ointment, can catch hydroxyl radical free radical and produce coloring matter in system, and this material has maximum absorption at 510nm place, and when there is OH free-radical scavengers in reaction system, this oxidising process is suppressed, and absorbance then reduces. At FeSO4With H2O2In reaction system, add the embodiment of the present invention 2 that concentration is 30mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 3, comparative example are cultivated, measure clearance rate.
(5) NO when simulated gastric fluid pH2-Cleaning reaction effect: cleaning nitrite is one of approach of effectively preventing N-nitroso compound carcinogenic. Simulated system adds and adds the embodiment of the present invention 2 that concentration is 14mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 3, comparative example are cultivated, measure clearance rate.
(6) DPPH method measures Cordyceps militaris (L.) Link. anti-oxidant activity: DPPH is a kind of stable free radical in organic solvent, extensively in anti-oxidant appraisement system. In system, add the embodiment of the present invention 2 that concentration is 30mg/ml respectively, Cordyceps militaris (L.) Link. crude extract that embodiment 3, comparative example are cultivated, measure clearance rate.
3, test-results:
The reducing power of the Cordyceps militaris (L.) Link. crude extract that table 2 different culture media is cultivated
| Embodiment 2 | Embodiment 3 | Comparative example | |
| Light absorption value | 1.522 | 1.563 | 0.962 |
Table 2 result shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 2, embodiment 3, comparative example are cultivated has oxidation-resistance, and embodiment 2, embodiment 4 oxidation-resistance are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 3 different culture media is cultivated removes the ability of superoxide anion
| Embodiment 2 | Embodiment 3 | Comparative example | |
| Clearance rate | 30% | 33% | 24% |
Table 3 shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 2, embodiment 3, comparative example are cultivated all has the ability removing superoxide anion, and embodiment 2, embodiment 3 Scavenging activity are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 4 different culture media is cultivated removes the ability of hydroxyl radical free radical
| Embodiment 2 | Embodiment 3 | Comparative example | |
| Clearance rate | 76% | 78% | 58% |
Table 4 shows, the Cordyceps militaris (L.) Link. crude extract that embodiment 2, embodiment 3, comparative example are cultivated all has the ability removing hydroxyl radical free radical, and embodiment 2, embodiment 3 Scavenging activity are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 5 different culture media is cultivated is to NO2-scavenging(action)
| Embodiment 2 | Embodiment 3 | Comparative example | |
| Clearance rate | 61% | 63% | 40% |
Table 5 shows, NO2-is had scavenging(action) by the Cordyceps militaris (L.) Link. crude extract that embodiment 2, embodiment 3, comparative example are cultivated, and embodiment 2, embodiment 3 Scavenging activity are better than comparative example.
The Cordyceps militaris (L.) Link. crude extract that table 6 different culture media is cultivated is to DPPH elimination effect
| Embodiment 2 | Embodiment 3 | Comparative example | |
| Clearance rate | 67% | 71% | 42% |
Table 6 shows, DPPH is had elimination effect by the Cordyceps militaris (L.) Link. crude extract that embodiment 2, embodiment 3, comparative example are cultivated, embodiment 2, embodiment 3 elimination effect are better than comparative example, show that the Cordyceps militaris (L.) Link. adding the Cordyccps-militaris-(L.)-link. Sporophore culture medium culturing of shaddock skin has good oxidation-resistance.
Summary oxidizing reaction system test-results, the Cordyceps militaris (L.) Link. that known embodiment 2, embodiment 3, comparative example are cultivated has oxidation-resistance, and embodiment 2, embodiment 3 are better than comparative example, illustrate that the Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin can strengthen the anti-oxidation efficacy of Cordyceps militaris (L.) Link..
Claims (5)
1. one kind is added the Cordyccps-militaris-(L.)-link. Sporophore substratum of shaddock skin, it is characterised in that, it is made up of the raw material of following weight part: nutritive medium 43��53 parts, major ingredient 38��48 parts, dried silkworm chrysalis meal 6��13 parts, shaddock skin 8��17 parts.
2. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation shaddock skin according to claim 1, it is characterised in that, it is made up of the raw material of following weight part: nutritive medium 46��51 parts, major ingredient 40��44 parts, dried silkworm chrysalis meal 9��11 parts, shaddock skin 11��14 parts.
3. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation shaddock skin according to claim 1 and 2, it is characterised in that: described major ingredient is one or more in rice, wheat, corn, soya bean.
4. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation shaddock skin according to claim 1 and 2, it is characterized in that, described nutritive medium is made up of the raw material of following weight part: sugar 0.7��1.7 part, yeast extract paste 0.7��1.4 part, potassium primary phosphate 0.02��0.04 part, 0.02��0.04 part, magnesium sulfate, ammonium citrate 0.02��0.04 part, VITMAIN B1 0.002��0.004 part, 96��99 parts, water.
5. the Cordyccps-militaris-(L.)-link. Sporophore substratum of interpolation shaddock skin according to claim 1 and 2, it is characterised in that: described sugar is glucose or sucrose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410311197.0A CN104163684B (en) | 2014-06-30 | 2014-06-30 | A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410311197.0A CN104163684B (en) | 2014-06-30 | 2014-06-30 | A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104163684A CN104163684A (en) | 2014-11-26 |
| CN104163684B true CN104163684B (en) | 2016-06-01 |
Family
ID=51907689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410311197.0A Expired - Fee Related CN104163684B (en) | 2014-06-30 | 2014-06-30 | A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding shaddock skin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104163684B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104909869B (en) * | 2015-05-06 | 2018-07-10 | 刘鸣 | Culture medium and preparation method thereof and the method using its cultivation Cordyceps militaris |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102715012A (en) * | 2012-06-13 | 2012-10-10 | 镇江市食用菌研究所 | Cultivation method of Cordyceps militaris |
| CN102972201A (en) * | 2011-09-07 | 2013-03-20 | 严兴荣 | High-efficiency and fast cultivation technique of north worm grass |
| CN103880538A (en) * | 2014-03-26 | 2014-06-25 | 泗阳县农业科学研究所 | Culture medium for morchella and preparation method thereof |
-
2014
- 2014-06-30 CN CN201410311197.0A patent/CN104163684B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972201A (en) * | 2011-09-07 | 2013-03-20 | 严兴荣 | High-efficiency and fast cultivation technique of north worm grass |
| CN102715012A (en) * | 2012-06-13 | 2012-10-10 | 镇江市食用菌研究所 | Cultivation method of Cordyceps militaris |
| CN103880538A (en) * | 2014-03-26 | 2014-06-25 | 泗阳县农业科学研究所 | Culture medium for morchella and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104163684A (en) | 2014-11-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105077261B (en) | Compound fruit and vegetable ferment and preparation method thereof | |
| CN103820340B (en) | A kind of yeast rich in selenium and preparation method thereof | |
| CN105174493B (en) | A kind of preparation method and applications of fermented type Chinese herbal medicine improver of water quality used for aquiculture | |
| CN111249218B (en) | Saussurea involucrate fermentation stock solution and preparation method and application thereof | |
| CN102210449A (en) | Liquid submerged fermentation culture of Thelephora ganbajun Zang and preparation methods of seasoning and selenium-enriched product of Thelephora ganbajun Zan | |
| CN104621414B (en) | Laying hen feed additive for producing lutein-enriched eggs by using orange peels and preparation method | |
| CN111642622A (en) | Preparation and application of fermentation composite bacteria and myrtle fermentation extract | |
| CN101508961A (en) | Method of preparing yeast rich in copper | |
| CN110916177A (en) | Method for preparing kelp enzyme by enzyme fermentation coupling technology | |
| CN103315220B (en) | Selenium-rich monascus production method | |
| CN103783421B (en) | Preparation method of selenium-enriched fermentation osmunda japonica thunb | |
| CN104030843B (en) | A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding hop residue | |
| CN109568518B (en) | Solid fermentation method of traditional Chinese medicine mixed bacteria, obtained fermented traditional Chinese medicine and application thereof | |
| CN104151014A (en) | Auricularia auricula culture medium added with trollflower residues | |
| CN110257296B (en) | Culture method and application of chromium-enriched spirulina | |
| CN103948023B (en) | The health food of a kind of develop immunitypty and improving water flood and two-step fermentation preparation method thereof | |
| CN104030842A (en) | Agaric cultivation medium with jasmine flower residue | |
| CN104030851A (en) | Lentinula edodes culture medium in which shaddock peel is added | |
| CN104163685B (en) | A kind of Cordyceps militaris (L.) Link.sporophore culture medium adding Flos Trollii slag | |
| US20210321643A1 (en) | Method for preparation of nitrite ion-containing allium tuberosum fermentate and composition thereof | |
| CN105462743A (en) | Phyllanthus emblica fruit wine preparation method and Phyllanthus emblica fruit wine prepared through method | |
| CN104711203A (en) | Selenium-rich germanium yeast powder | |
| CN104082528A (en) | Feed additive and preparation method thereof | |
| CN104163686A (en) | A lentinula edodes culture medium comprising jasmine flower broken bits | |
| CN105819967A (en) | Nutrient rich in selenium and iodine, preparing method thereof and method for cultivating tomato rich in selenium and iodine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20181226 Address after: 400900 "Hongsheng Square" 6A39, Tangxiang Street Office, Dazu District, Chongqing Patentee after: Chongqing Dazu Bianzhe Ecological Agriculture Co., Ltd. Address before: 541200 Guangxi Zhuang Autonomous Region Fengmaoyuan Agricultural Technology Development Co., Ltd. Qingshitan Licorice Village, Lingchuan County, Guilin City Patentee before: Luxuriant source, Guilin agrotechnique development corporation, Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160601 Termination date: 20210630 |