CN104161994A - Preparation method of high-activity gymnadenia conopsea - Google Patents
Preparation method of high-activity gymnadenia conopsea Download PDFInfo
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- CN104161994A CN104161994A CN201410418439.6A CN201410418439A CN104161994A CN 104161994 A CN104161994 A CN 104161994A CN 201410418439 A CN201410418439 A CN 201410418439A CN 104161994 A CN104161994 A CN 104161994A
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- rhizoma gymnadeniae
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Abstract
The invention relates to a preparation method of high-activity gymnadenia conopsea. The method comprises the steps of taking freeze-dried gymnadenia conopsea, putting the freeze-dried gymnadenia conopsea into cow milk or goat milk to soak, taking out, and drying. The pharmacological activity of prepared gymnadenia conopsea is high.
Description
Invention field
The present invention relates to medicine method for making, particularly a kind of preparation method of high activity Rhizoma Gymnadeniae, belongs to medical technical field.
Background technology
Traditional Chinese medicine drying has very long history in China, the Chinese herbal medicine of drying can keep certain medicinal ingredient and be difficult for putrid and deteriorated, the dry important measures as guaranteeing Chinese herbal medicine quality, it is a requisite technical process in Chinese herbal medicine processing, it is in close relations that dry and Chinese medicine are produced, and dry method will directly affect the quality of product.
Rhizoma Gymnadeniae is the tuber of the orchid family herbaceos perennial Rhizoma Gymnadeniae (Gymnadenia conopsea R.Br) or gymnadenia crassinervis Finet (Gymnedia crassinernis Finer).Have another name called Fructus Citri Sarcodactylis ginseng, palm ginseng.There is the effects such as invigorating the spleen to benefit the lung, nourishing blood to promote the production of body fluid, hemostasis with astringents, clinical Chang Peiwu be applied to treatment after being ill weak, chronic diarrhea, lactogenesis, lose blood, the disease such as leukorrhagia, chronic hepatitis.
Traditional preparation method of Rhizoma Gymnadeniae has two kinds, and a kind of in order Rhizoma Gymnadeniae is put in cold water and to be cleaned, taking-up is dried, and another kind is that the ginseng of washing one's hands is put in sheep or milk with 1:1 ratio and boiled to the saturating heart, takes out, and dries.The second is the Rhizoma Gymnadeniae preparation method of modern mongolian medicine extensive use.
Summary of the invention
The object of this invention is to provide the drying means that a kind of Rhizoma Gymnadeniae is new, the dry Rhizoma Gymnadeniae Product Activity of the method is high.
The object of the present invention is achieved like this:
A preparation method for high activity Rhizoma Gymnadeniae, is characterized in that: gets lyophilizing Rhizoma Gymnadeniae, puts in milk or sheep milk and soak into, take out, and dry.
The preparation method of a kind of high activity Rhizoma Gymnadeniae of the present invention, lyophilizing Rhizoma Gymnadeniae is to get fresh Rhizoma Gymnadeniae, is refrigerated to below-20 ℃, be evacuated to below drying chamber pressure 30Pa, subliming by heating, the temperature of distillation is controlled at below 40 ℃, resolution temperature is controlled at below 45 ℃, to dry.
So far completed the present invention, Rhizoma Gymnadeniae pharmacologically active prepared by method of the present invention is high.
Below by pharmacodynamic study, further illustrate effect of the present invention, test is intended to further illustrate usefulness of the present invention, but not limitation of the present invention.
One, will, with a collection of fresh Rhizoma Gymnadeniae, process by the following method.
1) dry Rhizoma Gymnadeniae: fresh Rhizoma Gymnadeniae is put in cold water and cleaned, and taking-up is dried;
2) milk Rhizoma Gymnadeniae: fresh Rhizoma Gymnadeniae is put in cold water and cleaned, put in milk and boil to the saturating heart with 1:1 ratio, take out, dry;
3) freeze-dried milk Rhizoma Gymnadeniae: fresh Rhizoma Gymnadeniae is put in cold water and cleaned, put in water and boil to the saturating heart, take out, be refrigerated to below-20 ℃, be evacuated to below drying chamber pressure 45Pa, subliming by heating, the temperature of distillation should be controlled at below 40 ℃, resolution temperature is controlled at below 45 ℃, to dry, take out, put in milk and soak and boil to the saturating heart, take out, dry.
Two, the dry Rhizoma Gymnadeniae pharmacodynamics comparison of distinct methods
1, to hepatitis B virus surface antigen inhibitory action
1) material V-type Microhemagglutination Sptting plate; HBsAg adopts HBV hepatitis serum, through anti-phase passive hemagglutination assay strong positive titre (1:3200); The anti-HB-sIgG sensitized erythrocyte of purification, Polyethylene Glycol (molecular weight 12000), phosphate buffer dilution Ox blood serum, suppresses antibody etc.
2) 1. method gets micro plate at the bottom of 96 hole V-types, respectively ranked first pipet for hole and moves into the dry Rhizoma Gymnadeniae water extraction liquid (1mg/mL) of distinct methods and virus antigen body, shakes up.Respectively ranked second Kong Xianyong pipet and add each 25m1 of diluent, then draw medicine-virus liquid doubling dilution from the first hole.Get diluent 5mL, add lyophilizing hepatitis B virus table and diagnostic antigen with in 1, blood cell, after shaking up, splash into each 25m1 of 2-4 hole, then add diluent 25m1, be placed in 37 ℃ of calorstats, 4h observes.2. positive-virus, negative virus, trial drug, blank medicine control wells are all established in the every batch of experiment of dividing into groups.
3) result judges that not coagulation appears in high dilution, and prompting medicine suppresses virus antigen, and 2 times of inhibition are poor efficiency, and 4 times of inhibition are middle effect, and 8 times of inhibition are efficient.
4) after the dry Rhizoma Gymnadeniae water extraction liquid 4h of result distinct methods, drying Rhizoma Gymnadeniae and fresh Rhizoma Gymnadeniae and do not occur suppressing, there are 2 times of inhibition in milk Rhizoma Gymnadeniae, and 8 times of inhibition appear in freeze-dried milk Rhizoma Gymnadeniae.Result shows, freeze-dried milk Rhizoma Gymnadeniae has efficient inhibitory action to hepatitis B virus surface antigen.
2, the dry Rhizoma Gymnadeniae of distinct methods causes the protective effect comparison of gastric mucosa damage to ethanol.
Choose healthy SD rat, male and female half and half, weight 180-220g, be divided at random matched group, dry Rhizoma Gymnadeniae group, milk boils Rhizoma Gymnadeniae group, milk boils lyophilizing Rhizoma Gymnadeniae group, decocting in water lyophilizing Rhizoma Gymnadeniae group, totally 5 groups, 10 every group, every day, gastric infusion was 1 time, 1 2mg/kg, continuous 7 days, matched group gave equivalent distilled water.Before experiment, first fasting 48 h of rat, freely drink water, after last administration, every rat oral gavage dehydrated alcohol 1mL/ of 1h only, after the de-cervical vertebra of 1h, put to death animal, by clip, close cardia, from pylorus, inject 1% formaldehyde 5mL, folder closes pylorus again, put into fixedly 10min of 1% formalin, along greater gastric curvature, cut off coat of the stomach, measure damaged length, using damaged length (width is greater than 1 mm and doubles) summation as gastric mucosal damage index, and calculate suppression ratio.The results are shown in Table 1.
Suppression ratio (%)=(matched group ulcer index one administration group ulcer index) ÷ matched group ulcer index * 100%.
The dry Rhizoma Gymnadeniae of table 1 distinct methods causes the protective effect comparison of gastric mucosa damage to ethanol
| Group | Dosage (mg/kg) | Gastric mucosal damage index (mm) | Suppression ratio (%) |
| Matched group | ? | 93.9±36.4 | ? |
| Dry Rhizoma Gymnadeniae group | 2 | 62.5±28.4 | 33.4 |
| Milk Rhizoma Gymnadeniae group | 2 | 51.3±24.2 | 45.4 |
| Freeze-dried milk Rhizoma Gymnadeniae group | 2 | 37.4±20.4 | 60.2 |
Result shows, the dry Rhizoma Gymnadeniae of distinct methods causes gastric mucosa damage to ethanol all protective effect, and wherein, the protective effect of freeze-dried milk Rhizoma Gymnadeniae is best.
The specific embodiment
embodiment 1
Get fresh Rhizoma Gymnadeniae, be refrigerated to-20 ℃, be evacuated to drying chamber pressure 30Pa, subliming by heating, sublimation temperature is controlled at below 40 ℃, and resolution temperature is controlled at below 45 ℃, to dry, takes out, and puts in milk and soaks and boil to the saturating heart, takes out, and dries.
embodiment 2
Get fresh Rhizoma Gymnadeniae, put in freeze dryer, be refrigerated to-28 ℃, be evacuated to drying chamber pressure 50Pa, subliming by heating, sublimation temperature is controlled at 50 ℃, resolution temperature is controlled at below 45 ℃, to dry, takes out, put in milk and soak and boil to the saturating heart, take out, dry, take out, put in milk and soak and boil to the saturating heart, take out, dry.
embodiment 3
Get fresh Rhizoma Gymnadeniae, put in freeze dryer, be refrigerated to-32 ℃, be evacuated to drying chamber pressure 50Pa, subliming by heating, sublimation temperature is controlled at 50 ℃, and resolution temperature is controlled at below 45 ℃, to dry, takes out, and puts in sheep milk and soaks and boil to the saturating heart, takes out, and dries.
embodiment 4
Get fresh Rhizoma Gymnadeniae, put in freeze dryer, be refrigerated to-25 ℃, be evacuated to drying chamber pressure 60Pa, start heater switch, promote distillation, the temperature of distillation is controlled at 40 ℃, and to distillation end, resolution temperature is controlled at below 45 ℃, to dry, take out, put in sheep milk and soak and boil to the saturating heart, take out, dry.
Claims (2)
1. a preparation method for high activity Rhizoma Gymnadeniae, is characterized in that: get lyophilizing Rhizoma Gymnadeniae, put in milk or sheep milk and soak into, take out, be dried.
2. according to the preparation method of a kind of high activity Rhizoma Gymnadeniae of claim 1, lyophilizing Rhizoma Gymnadeniae is to get fresh Rhizoma Gymnadeniae, is refrigerated to below-20 ℃, be evacuated to below drying chamber pressure 30Pa, subliming by heating, the temperature of distillation is controlled at below 40 ℃, resolution temperature is controlled at below 45 ℃, to dry.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410418439.6A CN104161994A (en) | 2014-08-25 | 2014-08-25 | Preparation method of high-activity gymnadenia conopsea |
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| CN201410418439.6A CN104161994A (en) | 2014-08-25 | 2014-08-25 | Preparation method of high-activity gymnadenia conopsea |
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| CN201410418439.6A Pending CN104161994A (en) | 2014-08-25 | 2014-08-25 | Preparation method of high-activity gymnadenia conopsea |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103977034A (en) * | 2014-05-15 | 2014-08-13 | 济南康众医药科技开发有限公司 | Drying method of medicinal fungi |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103977034A (en) * | 2014-05-15 | 2014-08-13 | 济南康众医药科技开发有限公司 | Drying method of medicinal fungi |
Non-Patent Citations (2)
| Title |
|---|
| 格格日勒 等: "蒙药材手参研究概况", 《亚太传统医药》 * |
| 蒋灵芝 等: "手参散剂治疗急性胃溃疡的实验研究", 《中药材》 * |
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Application publication date: 20141126 |