CN104130936A - Oscillation type single cell separator - Google Patents
Oscillation type single cell separator Download PDFInfo
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- CN104130936A CN104130936A CN201410401533.0A CN201410401533A CN104130936A CN 104130936 A CN104130936 A CN 104130936A CN 201410401533 A CN201410401533 A CN 201410401533A CN 104130936 A CN104130936 A CN 104130936A
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- rotator
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- upper strata
- kapillary
- single cell
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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Abstract
The invention provides an oscillation type single cell separator. The oscillation type single cell separator comprises a bottom-layer oscillator, an upper-layer rotator and capillaries, wherein an inverted T shape is formed by the bottom-layer oscillator and the upper-layer rotator, the bottom-layer oscillator is disc-shaped, the upper-layer rotator is cylinder-shaped, at least one circle of capillaries is arranged on the upper-layer rotator, the tubing of the capillaries is of an S-shaped bent shape, the capillaries are made from glass, a vibrating motor is arranged in the bottom-layer oscillator, the bottom-layer oscillator is connected with the upper-layer rotator, and a rotating motor is arranged at the upper part of the internal cavity of the upper-layer rotator; the at least one circle of capillaries is fixed on the rotator through fixed snapping buckles outside the upper-layer rotator in a clockwise spiral falling manner. The oscillation type single cell separator is used for separating single cells from a cell suspension; the at least one circle of capillaries is arranged on the upper-layer rotator, and the tubing of the capillaries is of an S-shaped bent shape, so that the single cells are separated from the cell suspension in the tubing under the oscillation of the bottom-layer oscillator and the rotation of the upper-layer rotator.
Description
Technical field
The present invention relates to a kind of oscillation mode single cell separator, belong to biological medicine plant and instrument.
Background technology
The current research based on unicellular level more and more comes into one's own and becomes a kind of trend of research from now on.The fields such as single celled DNA cloning, gene order-checking and the research of unicellular horizontal ubcellular drug delivery system have been widely used in.And the successful solution of single-cell technique is the basis of realizing unicellular level research.How effectively acquisition is unicellular is usually perplexing investigator further to study its biological characteristics problem.
Traditional method is exactly repeated multiple times limiting dilution assay, limiting dilution assay cell dilution to every 2 ~ 5 μ L of theoretic 1 cell, then with the cell suspension that pipettor is drawn corresponding volume, carry out unicellular separation.But in actual operation, this method efficiency is not high, generally in 20% left and right, a large amount of blank well and many cells hole (cell count is more than or equal to 2) that exist need researchist to observe eliminating, waste time and energy.This working method is simple, but whole Period Process is long, wastes time and energy, and can not guarantee obtaining of individual cells.
Unicellular micrurgy, pipettor volume inconvenient operation under the microscope bigger than normal, and poor stability are moved liquid point tip diameter too large in addition, a plurality of cells of different aspects under the large and easy inspiration field of microscope of liquid absorption.
At present high-end flow cytometer not only can be realized the hyperchannel sorting of viable cell (fluorescence-activated cell sorting FACS), can also carry out single celled separated plantation sorting, for technical foundation is established in large-scale unicellular research.But these size separation equipments are expensive, be also difficult at present popularize.Due to FACS process because relate to digestion and the sorting of cell, thereby may exert an influence and affect the accuracy of experimental result the activity of cell and surface markers.And flow cytometer obtains is the mean value of cell colony information, can not reflect that the Physiology and biochemistry of unicellular inside changes.
Above the whole bag of tricks, the not high or inconvenient operation or expensive of some method efficiency, easy damaging cells, therefore, explores that one is simple to operate, unicellular separation method is very meaningful fast and efficiently.
Summary of the invention
In order to address the above problem, further simplify technology mode, handled easily, improves accuracy and efficiency, alleviates workload, damaging cells not, convenient detect unicellular interior biochemical reactions, the invention provides a kind of oscillation mode single cell separator, can sharp separation unicellular, can aseptic technique in Biohazard Safety Equipment, use after each part detachable of separator, sterilizing, simple to operate and to the not damaged of cell own, also help follow-up cultivation and the detection of cell.
Technical scheme of the present invention is:
A kind of oscillation mode single cell separator, comprise bottom vibrator, upper strata rotator and kapillary, bottom vibrator and upper strata rotator form inverted T shape, and bottom vibrator is discoid, upper strata rotator is cylindrical shape, on the rotator of upper strata, be provided with at least one circle kapillary, kapillary pipeline is S type bending, and kapillary is glass material, in bottom vibrator, be provided with vibrating motor and be connected with upper strata rotator, the top of upper strata rotator inner chamber is provided with rotation motor.
At least one circle kapillary is fixed on rotator by the clockwise spiral decline of fixedly buckle of rotator outside, upper strata.
At least one circle material capillaceous can be glass material.
At least one circle internal diameter of the pipeline capillaceous is decreased to the size matching with cell gradually to spout from influx.
The invention has the beneficial effects as follows: the present invention is unicellular in order to isolate from cell suspending liquid.By at least one circle kapillary arranging on the rotator of upper strata, the S-type bending of its pipeline, the cell suspending liquid in pipeline, under the vibration of bottom vibrator and the rotation of upper strata rotator, is separated from cell suspending liquid unicellular.
Accompanying drawing explanation
Fig. 1 is the front view of oscillation mode single cell separator;
Fig. 2 is the sectional view of oscillation mode single cell separator;
Fig. 3 is the fine structure map of glass capillary inside;
Fig. 4 is that oscillation mode single cell separator is isolated state graph when unicellular.
In figure: 10-bottom vibrator, 101-spring, 102-base, 103-bearing, 104-vibrating motor, 20-upper strata rotator, 201-fixing buckle, 202-rotation motor, 203-tightening cover, 30-kapillary, 301-add entrance, 302-spout, 40-unicellular.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the drawings and specific embodiments, describe the present invention.
As shown in Figures 1 to 4, a kind of oscillation mode single cell separator of the present invention, comprise bottom vibrator 10, upper strata rotator 20 and kapillary 30, bottom vibrator 10 and upper strata rotator 20 form inverted T shape, bottom vibrator 10 is discoid, upper strata rotator 20 is cylindrical shape, on upper strata rotator 20, be provided with at least one circle kapillary 30, kapillary 30 pipelines are S type bending, kapillary 30 is glass material, in bottom vibrator 10, be provided with vibrating motor 104 and be connected with upper strata rotator 20, the top of upper strata rotator 20 inner chambers is provided with rotation motor 202.
Kapillary 30 is fixed on upper strata rotator 20 by the clockwise spiral decline of fixedly buckle 201 of upper strata rotator 20 outsides.
The material of kapillary 30 is glass material.In present embodiment, kapillary 30 materials are quartzy.
The internal diameter of the pipeline of kapillary 30 is decreased to the size matching with cell gradually to spout from influx.
Bottom vibrator 10 and described upper strata rotator 20 materials are stainless steel or metal.In present embodiment, bottom vibrator 10 and upper strata rotator 20 materials are metallic aluminium.
Bottom vibrator 10 outsides comprise base 102 and spring 101, inner involving vibrations motor 104 and bearing 103, and bottom vibrator 10 vibrates up and down by vibrating motor 104.
Upper strata rotator 20 comprises outside fixedly buckle 201, inner rotation motor 202 and tightening cover 203, and upper strata rotator 20 is rotated counterclockwise under the effect of rotation motor 202.Upper strata rotator 20 is connected by bearing 103 with bottom vibrator 10.
Kapillary 30 is from top to bottom fixed on upper strata rotator 20 clockwise by fixing buckle 201, and kapillary 30 internal pipelines are S type bending, make cell marshalling therein.In present embodiment, there are four circle kapillaries to be fixed on upper strata rotator 20 by fixing buckle 201.
Cell suspending liquid flows into kapillary 30 from adding entrance 301, under the turning effort of the interior vibration through bottom vibrator 10 of kapillary 30 and upper strata rotator 20, cell is arranged in the pipeline of the inner S type of kapillary bending, and oozing from spout 302 in the effect of gravitional force next each and every one, thereby isolate unicellularly 40, guarantee aseptic, simple, easy to operate, damaging cells not in process.The arc diameter of kapillary 30 is divided into different size according to cell size, as 100 μ m, and 150 μ m, 200 μ m etc., thus reduce Flow Velocity under liquid, be beneficial to unicellular separation.
Oscillation mode single cell separator in present embodiment, after obtaining single intact cell, can be used for single celled photoelectric synchronous and detects.
In sum, oscillation mode single cell separator of the present invention is by being fixed at least one circle kapillary 30 on upper strata rotator 20, the pipeline of S type bending, the rotation of the vibration of bottom vibrator 10 and upper strata rotator 20 by least one circle kapillary 30 can easily be separated from cell suspending liquid unicellular.Oscillation mode single cell separator of the present invention can operate in Biohazard Safety Equipment, guarantees aseptic technique, and described kapillary is detachable, after sterilizing, uses.Oscillation mode single cell separator of the present invention requires wider to operator's operating skill, can greatly alleviate research staff's time and efforts, simultaneously can also sharp separation individual cells, and to the not damaged of cell own, also the later stage of cell is cultivated and detected without impact.
Claims (4)
1. an oscillation mode single cell separator, it is characterized in that: comprise bottom vibrator, upper strata rotator and kapillary, described bottom vibrator and described upper strata rotator form inverted T shape, described bottom vibrator is discoid, described upper strata rotator is cylindrical shape, on the rotator of described upper strata, be provided with at least one circle kapillary, kapillary pipeline is S type bending, kapillary is glass material, in bottom vibrator, be provided with vibrating motor and be connected with upper strata rotator, the top of described upper strata rotator inner chamber is provided with rotation motor.
2. oscillation mode single cell separator according to claim 1, is characterized in that: described at least one circle kapillary is fixed on rotator by the clockwise spiral decline of fixedly buckle of rotator outside, upper strata.
3. oscillation mode single cell separator according to claim 1, is characterized in that: described at least one circle material capillaceous is glass material.
4. oscillation mode single cell separator according to claim 1, is characterized in that: described at least one circle internal diameter of the pipeline capillaceous is decreased to the size matching with cell gradually to spout from influx.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410401533.0A CN104130936B (en) | 2014-08-14 | 2014-08-14 | Oscillation mode single cell separator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410401533.0A CN104130936B (en) | 2014-08-14 | 2014-08-14 | Oscillation mode single cell separator |
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| CN104130936A true CN104130936A (en) | 2014-11-05 |
| CN104130936B CN104130936B (en) | 2016-01-27 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87209860U (en) * | 1987-07-08 | 1988-05-25 | 中国人民解放军总医院 | Single cell separator |
| WO2010113994A1 (en) * | 2009-03-31 | 2010-10-07 | 財団法人神奈川科学技術アカデミー | Device for concentrating and separating cells |
| CN103114070A (en) * | 2013-03-08 | 2013-05-22 | 博生吉医药科技(苏州)有限公司 | Application method of single-cell separator |
| CN103160428A (en) * | 2013-03-08 | 2013-06-19 | 博生吉医药科技(苏州)有限公司 | Unicell separator |
| WO2013147114A1 (en) * | 2012-03-30 | 2013-10-03 | 公益財団法人神奈川科学技術アカデミー | Imaging cell sorter |
| CN203411544U (en) * | 2013-04-25 | 2014-01-29 | 颜凤 | Aorta three-dimensional culture single cell separator |
| CN203976785U (en) * | 2014-08-14 | 2014-12-03 | 江苏瑞明生物科技有限公司 | Oscillation mode single cell separator |
-
2014
- 2014-08-14 CN CN201410401533.0A patent/CN104130936B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87209860U (en) * | 1987-07-08 | 1988-05-25 | 中国人民解放军总医院 | Single cell separator |
| WO2010113994A1 (en) * | 2009-03-31 | 2010-10-07 | 財団法人神奈川科学技術アカデミー | Device for concentrating and separating cells |
| US20120088295A1 (en) * | 2009-03-31 | 2012-04-12 | Kanagawa Academy Of Science And Technology | Device for concentrating and separating cells |
| WO2013147114A1 (en) * | 2012-03-30 | 2013-10-03 | 公益財団法人神奈川科学技術アカデミー | Imaging cell sorter |
| CN103114070A (en) * | 2013-03-08 | 2013-05-22 | 博生吉医药科技(苏州)有限公司 | Application method of single-cell separator |
| CN103160428A (en) * | 2013-03-08 | 2013-06-19 | 博生吉医药科技(苏州)有限公司 | Unicell separator |
| CN203411544U (en) * | 2013-04-25 | 2014-01-29 | 颜凤 | Aorta three-dimensional culture single cell separator |
| CN203976785U (en) * | 2014-08-14 | 2014-12-03 | 江苏瑞明生物科技有限公司 | Oscillation mode single cell separator |
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| CN104130936B (en) | 2016-01-27 |
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