CN104130936B - Oscillation mode single cell separator - Google Patents
Oscillation mode single cell separator Download PDFInfo
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- CN104130936B CN104130936B CN201410401533.0A CN201410401533A CN104130936B CN 104130936 B CN104130936 B CN 104130936B CN 201410401533 A CN201410401533 A CN 201410401533A CN 104130936 B CN104130936 B CN 104130936B
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- rotator
- upper strata
- kapillary
- circle
- bottom vibrator
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The invention provides a kind of oscillation mode single cell separator, comprise bottom vibrator, upper strata rotator and kapillary, bottom vibrator and upper strata rotator composition inverted T shape, bottom vibrator is discoid, upper strata rotator is cylindrical shape, upper strata rotator is provided with at least one circle kapillary, capillary duct is S type bending, and kapillary is glass material, be provided with vibrating motor in bottom vibrator and be connected with upper strata rotator, the top of upper strata rotator inner chamber is provided with rotation motor.At least one circle kapillary is declined by the fixing buckle clock wise spirals of rotator outside, upper strata and is fixed on rotator.The present invention is unicellular in order to isolate from cell suspending liquid.By at least one circle kapillary arranged on the rotator of upper strata, the S-type bending of its pipeline, the cell suspending liquid in pipeline, under the vibration of bottom vibrator and the rotation of upper strata rotator, is separated unicellular from cell suspending liquid.
Description
Technical field
The present invention relates to a kind of oscillation mode single cell separator, belong to biological medicine plant and instrument.
Background technology
The current research based on individual cell level more and more comes into one's own and becomes a kind of trend studied from now on.The fields such as single celled DNA cloning, gene order-checking and the research of individual cell level ubcellular drug delivery system are widely used in.And the successful solution of single-cell technique is the basis realizing individual cell level research.How effectively acquisition is unicellular usually annoyings investigator to study its biological characteristics sex chromosome mosaicism further.
Traditional method is exactly repeated multiple times limiting dilution assay, limiting dilution assay namely cell dilution to every 2 ~ 5 μ L of theoretic 1 cell, then carry out unicellular separation with the cell suspension that pipettor draws corresponding volume.But in the operation of reality, this method efficiency is not high, generally about 20%, blank well and the many cells hole (cell count is more than or equal to 2) of a large amount of existence need researchist to observe eliminating, waste time and energy.This working method is simple, but whole Period Process is long, wastes time and energy, and can not ensure the acquisition of individual cells.
Unicellular micrurgy, pipettor volume inconvenient operation under the microscope bigger than normal, and poor stability, to move liquid point tip diameter too large in addition, the large and easy multiple cells of different aspects under inspiration field of microscope of liquid absorption.
Flow cytometer high-end at present not only can realize the hyperchannel sorting (fluorescence-activated cell sorting, FACS) of viable cell, can also carry out single celled separation plantation sorting, for technical foundation is established in large-scale unicellular research.But these size separation equipments are expensive, be also difficult at present popularize.Due to FACS process because relate to digestion and the sorting of cell, may have an impact on the activity of cell and surface markers thus affect the accuracy of experimental result.And flow cytometer obtains is the mean value of cell colony information, the physiological acoustic signals of unicellular inside can not be reflected.
Various method above, the not high or inconvenient operation or expensive of some method efficiency, easy damaging cells, therefore, explores that one simple to operate, unicellular separation method is very meaningful fast and efficiently.
Summary of the invention
In order to solve the problem, further simplification technology mode, handled easily, improves accuracy and efficiency, alleviates workload, not damaging cells, convenient detect unicellular interior biochemical reactions, the invention provides a kind of oscillation mode single cell separator, can sharp separation unicellular, can aseptic technique in Biohazard Safety Equipment, each part detachable of separator, use after sterilizing, simple to operate and to the not damaged of cell own, also help follow-up cultivation and the detection of cell.
Technical scheme of the present invention is:
A kind of oscillation mode single cell separator, comprise bottom vibrator, upper strata rotator and kapillary, bottom vibrator and upper strata rotator composition inverted T shape, bottom vibrator is discoid, upper strata rotator is cylindrical shape, upper strata rotator is provided with at least one circle kapillary, capillary duct is S type bending, and kapillary is glass material, be provided with vibrating motor in bottom vibrator and be connected with upper strata rotator, the top of upper strata rotator inner chamber is provided with rotation motor.
At least one circle kapillary is declined by the fixing buckle clock wise spirals of rotator outside, upper strata and is fixed on rotator.
The material of at least one circle kapillary can be glass material.
The internal diameter of the pipeline of at least one circle kapillary is decreased to the size matched with cell gradually to spout from influx.
The invention has the beneficial effects as follows: the present invention is unicellular in order to isolate from cell suspending liquid.By at least one circle kapillary arranged on the rotator of upper strata, the S-type bending of its pipeline, the cell suspending liquid in pipeline, under the vibration of bottom vibrator and the rotation of upper strata rotator, is separated unicellular from cell suspending liquid.
Accompanying drawing explanation
Fig. 1 is the front view of oscillation mode single cell separator;
Fig. 2 is the sectional view of oscillation mode single cell separator;
Fig. 3 is the fine structure map of glass capillary inside;
Fig. 4 is the state graph of oscillation mode single cell separator when isolating unicellular.
In figure: 10-bottom vibrator, 101-spring, 102-base, 103-bearing, 104-vibrating motor, 20-upper strata rotator, 201-fixing buckle, 202-rotation motor, 203-tightening cover, 30-kapillary, 301-add entrance, 302-spout, 40-unicellular.
Embodiment
In order to make the object, technical solutions and advantages of the present invention clearly, describe the present invention below in conjunction with the drawings and specific embodiments.
As shown in Figures 1 to 4, a kind of oscillation mode single cell separator of the present invention, comprise bottom vibrator 10, upper strata rotator 20 and kapillary 30, bottom vibrator 10 and upper strata rotator 20 form inverted T shape, bottom vibrator 10 is discoid, upper strata rotator 20 is cylindrical shape, upper strata rotator 20 is provided with at least one circle kapillary 30, kapillary 30 pipeline is S type bending, kapillary 30 is glass material, be provided with vibrating motor 104 in bottom vibrator 10 and be connected with upper strata rotator 20, the top of upper strata rotator 20 inner chamber is provided with rotation motor 202.
Kapillary 30 is declined by fixing buckle 201 clock wise spirals of upper strata rotator 20 outside and is fixed on upper strata rotator 20.
The material of kapillary 30 is glass material.In present embodiment, kapillary 30 material is quartz.
The internal diameter of the pipeline of kapillary 30 is decreased to the size matched with cell gradually to spout from influx.
Bottom vibrator 10 and described upper strata rotator 20 material are stainless steel or metal.In present embodiment, bottom vibrator 10 and upper strata rotator 20 material are metallic aluminium.
Bottom vibrator 10 outside comprises base 102 and spring 101, and inner involving vibrations motor 104 and bearing 103, bottom vibrator 10 is vibrated by vibrating motor about 104.
Upper strata rotator 20 comprises outside fixing buckle 201, and inner rotation motor 202 and tightening cover 203, upper strata rotator 20 is rotated counterclockwise under the effect of rotation motor 202.Upper strata rotator 20 is connected by bearing 103 with bottom vibrator 10.
Kapillary 30 is from top to bottom fixed on upper strata rotator 20 clockwise by fixing buckle 201, and kapillary 30 internal pipeline is S type bending, makes cell marshalling wherein.In present embodiment, four circle kapillaries are had to be fixed on upper strata rotator 20 by fixing buckle 201.
Cell suspending liquid flows into kapillary 30 from adding entrance 301, in kapillary 30 under the turning effort of the vibration of bottom vibrator 10 and upper strata rotator 20, cell arranges in the pipeline that the inner S type of kapillary bends, and the oozing from spout 302 of effect next each and every one of gravitional force, thus isolate unicellular 40, ensure aseptic, simply, easy to operate, not damaging cells in process.The arc diameter of kapillary 30 is divided into different size according to cell size, as 100 μm, and 150 μm, 200 μm etc., thus reduce liquid dropping velocity, be beneficial to unicellular separation.
Oscillation mode single cell separator in present embodiment, after the single intact cell of acquisition, can be used for single celled photoelectric synchronous and detects.
In sum, oscillation mode single cell separator of the present invention is by being fixed on upper strata rotator 20 by least one circle kapillary 30, the rotation of the pipeline bent by the S type of at least one circle kapillary 30, the vibration of bottom vibrator 10 and upper strata rotator 20, can easily be separated unicellular from cell suspending liquid.Oscillation mode single cell separator of the present invention can operate in Biohazard Safety Equipment, ensures aseptic technique, and described kapillary is detachable, uses after sterilizing.Oscillation mode single cell separator of the present invention requires wider to the operating skill of operator, greatly can alleviate the time and efforts of research staff, simultaneously can also sharp separation individual cells, and to the not damaged of cell own, also the later stage of cell is cultivated and detected without impact.
Claims (3)
1. an oscillation mode single cell separator, it is characterized in that: comprise bottom vibrator, upper strata rotator and kapillary, described bottom vibrator and described upper strata rotator composition inverted T shape, described bottom vibrator is discoid, described upper strata rotator is cylindrical shape, described upper strata rotator is provided with at least one circle kapillary, capillary duct is S type bending, kapillary is glass material, be provided with vibrating motor in bottom vibrator and be connected with upper strata rotator, the top of described upper strata rotator inner chamber is provided with rotation motor;
The internal diameter of the pipeline of described at least one circle kapillary is decreased to the size matched with cell gradually to spout from influx.
2. oscillation mode single cell separator according to claim 1, is characterized in that: described at least one circle kapillary is declined by the fixing buckle clock wise spirals of rotator outside, upper strata and is fixed on rotator.
3. oscillation mode single cell separator according to claim 1, is characterized in that: the material of described at least one circle kapillary is glass material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410401533.0A CN104130936B (en) | 2014-08-14 | 2014-08-14 | Oscillation mode single cell separator |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410401533.0A CN104130936B (en) | 2014-08-14 | 2014-08-14 | Oscillation mode single cell separator |
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| CN104130936A CN104130936A (en) | 2014-11-05 |
| CN104130936B true CN104130936B (en) | 2016-01-27 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87209860U (en) * | 1987-07-08 | 1988-05-25 | 中国人民解放军总医院 | Single cell separator |
| WO2010113994A1 (en) * | 2009-03-31 | 2010-10-07 | 財団法人神奈川科学技術アカデミー | Device for concentrating and separating cells |
| CN103114070A (en) * | 2013-03-08 | 2013-05-22 | 博生吉医药科技(苏州)有限公司 | Application method of single-cell separator |
| CN103160428A (en) * | 2013-03-08 | 2013-06-19 | 博生吉医药科技(苏州)有限公司 | Unicell separator |
| WO2013147114A1 (en) * | 2012-03-30 | 2013-10-03 | 公益財団法人神奈川科学技術アカデミー | Imaging cell sorter |
| CN203411544U (en) * | 2013-04-25 | 2014-01-29 | 颜凤 | Aorta three-dimensional culture single cell separator |
| CN203976785U (en) * | 2014-08-14 | 2014-12-03 | 江苏瑞明生物科技有限公司 | Oscillation mode single cell separator |
-
2014
- 2014-08-14 CN CN201410401533.0A patent/CN104130936B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87209860U (en) * | 1987-07-08 | 1988-05-25 | 中国人民解放军总医院 | Single cell separator |
| WO2010113994A1 (en) * | 2009-03-31 | 2010-10-07 | 財団法人神奈川科学技術アカデミー | Device for concentrating and separating cells |
| US20120088295A1 (en) * | 2009-03-31 | 2012-04-12 | Kanagawa Academy Of Science And Technology | Device for concentrating and separating cells |
| WO2013147114A1 (en) * | 2012-03-30 | 2013-10-03 | 公益財団法人神奈川科学技術アカデミー | Imaging cell sorter |
| CN103114070A (en) * | 2013-03-08 | 2013-05-22 | 博生吉医药科技(苏州)有限公司 | Application method of single-cell separator |
| CN103160428A (en) * | 2013-03-08 | 2013-06-19 | 博生吉医药科技(苏州)有限公司 | Unicell separator |
| CN203411544U (en) * | 2013-04-25 | 2014-01-29 | 颜凤 | Aorta three-dimensional culture single cell separator |
| CN203976785U (en) * | 2014-08-14 | 2014-12-03 | 江苏瑞明生物科技有限公司 | Oscillation mode single cell separator |
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| CN104130936A (en) | 2014-11-05 |
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