CN104120100B - Raw bacillus megaterium and the application in repairing dichloro quinolinic acid poisoning thereof in one strain - Google Patents
Raw bacillus megaterium and the application in repairing dichloro quinolinic acid poisoning thereof in one strain Download PDFInfo
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Abstract
The present invention relates to a strain bacillus megaterium and the application in repairing Nicotiana tabacum L. dichloro quinolinic acid poisoning thereof.Described bacillus megaterium (Bacillus megaterium) Q3 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), and deposit number is: CGMCC No.8576.The bacillus megaterium Q3 of the present invention can effectively degrade the concentration of low dichloro quinolinic acid, can be applicable to the water body by dichloroquinoline acid pollution and soil, and for alleviating the dichloro quinolinic acid poisoning on tobacco leaf production.
Description
Technical field
The invention belongs to pollution by pesticides microorganism remediation field, specifically, raw in relating to a strain
Bacillus megaterium and the application in repairing dichloro quinolinic acid poisoning thereof.
Background technology
Dichloro quinolinic acid (quinclorac), chemical name: 3,7-bis-chloro-8-Quinoline Carboxylic Acids,
Before being the bud of BASF Aktiengesellschaft's exploitation, Novel rice paddy herbicide after bud, because it has consumption
Less, lasting period length, the advantages such as barnyard grass specially good effect are widely used in agricultural production, mainly use
The anti-barnyard grass in rice field.Its structural formula is as follows:
Dichloro quinolinic acid is faintly acid, and in acid ground, degraded is slowly, makees easily to rear stubble sensitivity
Thing causes serious harm, particularly in Oryza sativa L. and Nicotiana tabacum L. crop rotation district, and two chloroquines of residual in soil
Under very low concentrations, (0.001mg/kg) just can be to Solanaceae, Cucurbitaceae, Umbelliferae etc. in quinoline acid
Plant growth produces harm, has a strong impact on yield and the quality of crop.
In soil, the main path of dichloroquinoline acid degradation has light degradation and microbial degradation, and light
Degraded is because sunlight penetration depth problem in soil is limited significantly.Research
Proving, microbial degradation is the key factor of degrading pesticide residue in environment.At present, domestic pass
Less in the report of dichloro quinolinic acid microbial degradation.Dong Junyu etc. (Pesticide Science journal, 2013,
15 (3): 316-322) in 2013 from soil concentration and separation to a strain dichloroquinoline acid degradation
Bacterium Q3, is identified as Alcaligenes, can be the dichloro quinolinic acid of 100mg/L by initial concentration
Degraded 70% in 7d；Fan Jun etc. (Chinese biological preventing and treating journal, 2013,29 (3): 431-436)
It is separated to a strain dichloro quinolinic acid efficient degrading bacteria QC06 in 2013, is identified as general Pseudomonas,
This bacterium can be by dichloroquinoline acid degradation more than 95% that initial concentration is 50mg/L in 7d.Slowly
Refined rosy clouds etc. (safety and environment journal, 2012,2:45-49) are in isolated one in 2012 strain
The efficient degrading bacteria (HN36) of dichloro quinolinic acid, this Pseudomonas is in Bordetella, 48h
Can be by the dichloroquinoline acid degradation 96% of 400mg/L.Lv Zhenmei etc. (Chinese Journal of Applied Ecology, 2004,
15 (4): 605-609) at the efficient degrading bacteria of isolated one strain dichloro quinolinic acid in 2004
(WZ1), identified its belongs to onion pseudomonas sp, can be by the dichloro of 1000mg/L in 11d
Quinolinic acid degraded 90%.The degradation bacteria more than separated is all that concentration and separation obtains from soil, and
The most domestic report that there is no about degradation bacteria raw in dichloro quinolinic acid.Relative to the bacterium in soil
Strain, endophyte has can grow life inside soil and plant simultaneously surely, and is less subject to the external world
The advantage of environmental disturbances, and endophyte can by produce auxin, siderophore etc. promote to plant
The growth of thing.
Summary of the invention
Raw dichloro quinolinic acid degradation bacteria strains in the invention provides a strain, to solving because of two chloroquines
The Nicotiana tabacum L. large area poisoning that the residual of quinoline acid causes, and be biological repairing polluted soil or water body
Foundation is provided.
The dichloro quinolinic acid degradation bacteria separated from poisoning tobacco root provided by the present invention, through mirror
Being set to bacillus megaterium (Bacillus megaterium.), numbering Q3, in December 13 in 2013
Day it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (to be called for short
CGMCC, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the micro-life of the Chinese Academy of Sciences
Thing institute, postcode 100101), preserving number is CGMCC No.8576.
Bacterial strain of the present invention has preferable degradation capability to dichloro quinolinic acid, according to strain morphology
Feature, physiological and biochemical property (Biolog microplate), 16S rDNA sequence homology analysis,
Bacterial strain Q3 is accredited as bacillus megaterium (Bacillus megaterium.).
The present invention also provides for a kind of microbial inoculum containing described bacillus megaterium Q3.
The preparation method of described microbial inoculum is: LB culture medium inoculated strain, 180r/min, sends out for 30 DEG C
Ferment is cultivated 3 days.It is prepared as liquid preparation or solid preparation according to different application aspect.
Invention also provides above-mentioned bacillus megaterium Q3 in degraded dichloro quinolinic acid
Application.
Being experimentally verified that, dichloro quinolinic acid is had relatively by bacillus megaterium Q3 of the present invention
Good degradation capability, it is possible to be applied to the degraded of dichloro quinolinic acid under multiple environment.
Specifically, above-mentioned application is including, but not limited to dichloro quinolinic acid in water body or soil
Degraded, and in the application repaired in Nicotiana tabacum L. dichloro quinolinic acid poisoning.
Bacterial strain of the present invention can be applicable to by the water body of dichloroquinoline acid pollution and repairing of soil, with
And for repairing the dichloro quinolinic acid poisoning on Nicotiana tabacum L..
In above-mentioned application, described bacillus megaterium Q3 can be prepared as microbial inoculum, be inoculated in
In water body, soil or base manure.The preparation method of described microbial inoculum is as it has been described above, concrete application shape
Formula and method do not limit, and those skilled in the art can select voluntarily.
In order to preferably obtain application effect, the present invention furthermore present preferable application form
With method.As for bacillus megaterium Q3 answering in repairing Nicotiana tabacum L. dichloro quinolinic acid poisoning
With, preferably adopt the following technical scheme that and described bacillus megaterium Q3 is prepared as liquid
Preparation, every ml of formulation contains 109～1012Individual spore, every 1 kg liquid preparation 600-800
The mixing of times clear water, carries out root irrigation after tobacco transplant.
For the dichloro quinolinic acid in bacillus megaterium Q3 degraded soil, preferably use such as
Lower technical scheme: described bacillus megaterium Q3 is prepared as solid powder, every g of formulation contains
Have 1012～1014Individual spore, before tobacco transplant, by 1.5～2.0 kilograms of solid preparations/mu dosage with
Base manure mixes, and executes to field soil.
Bacillus megaterium Q3 of the present invention is the concrete side of dichloroquinoline acid degradation in water
Method can refer to ordinary skill in the art means, and this is not particularly limited by the present invention.
Beneficial effects of the present invention: degradation bacteria Q3 of the present invention is the huge bud of plant endogenesis
Spore bacillus, compensate for the blank of the most raw bacillus megaterium degraded dichloro quinolinic acid.
And degradation bacteria Q3 of the present invention not only has the function of degraded dichloro quinolinic acid, moreover it is possible to have
Nicotiana tabacum L. is caused poisoning by effect preventing and treating dichloro quinolinic acid, reduces production loss, improves the yield of Nicotiana tabacum L.
And quality.
Accompanying drawing explanation
The colonial morphology of Fig. 1 bacterial strain Q3；
The stereoscan photograph of Fig. 2 bacterial strain Q3；
The phylogenetic tree of Fig. 3 bacterial strain Q3；
Fig. 4 bacterial strain Q3 prevents and treats the potted plant photo of Nicotiana tabacum L. poisoning, wherein: A is bacterial strain Q3 process, B
For comparison；
Fig. 5 bacterial strain Q3 preventing and treating Nicotiana tabacum L. poisoning field photo, wherein: A is that bacterial strain Q3 processes 11 days
After, B is the comparison of 11 days, and after C bacterial strain Q3 processes 22 days, D is the comparison of 22 days.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.Not
In the case of deviating from present invention spirit and essence, the inventive method, step or condition are made
Amendment or replacement, belong to the scope of the present invention.
If not specializing, technological means used in embodiment is ripe by those skilled in the art
The conventional means known.
The separation of embodiment 1 bacterial strain and qualification
The present invention uses plate streak from by dichloro quinolinic acid poisoning tobacco root isolated
One strain has interior raw degradation bacteria Q3 of preferable degradation effect to dichloro quinolinic acid, Q3 colonial morphology and
Stereoscan photograph is as shown in Figure 1 and Figure 2.
1, culture medium:
Minimal medium: ammonium sulfate 1g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, chlorination
Sodium 0.1g, calcium carbonate 0.5g, 1000ml deionized water, pH7.0, high pressure steam sterilization 121
DEG C, 30min.
LB culture medium: Carnis Bovis seu Bubali cream 10g, peptone 5g, sodium chloride 10g (solid medium
Add agar 15g), 1000mL deionized water, pH7.0, high pressure steam sterilization 121 DEG C, 30min.
2, interior raw degradation bacteria is isolated and purified
Take by dichloro quinolinic acid poisoning tobacco root, rinse root soil well with tap water
After, with 75% ethanol rinsing 3-5min, aseptic water washing 3-4 time, 0.1%HgCl2Rinsing 3-5
Min, aseptic water washing 3-4 time.Take last aseptic water washing liquid 0.5ml painting LB to put down
Plate, cultivates 48h, has seen whether that bacterium colony produces, and checking surface sterilization is the most thorough.0.5
G sample proceeds to sterile mortar, adds 10ml sterilized water and grinds, and takes 50 μ l and is coated with containing two chloroquines
The inorganic salt flat board of quinoline acid, cultivates in 28 DEG C of incubators, can be formed on minimal medium
Colonies typical be labeled as dichloro quinolinic acid degradation bacteria, altogether isolated 6 strain degradation bacteria.Through many
Secondary switching after purification, uses high performance liquid chromatography to measure degradation bacteria respectively to dichloro quinolinic acid
Degradation effect, the Q3 bacterial strain taking degradation effect best carries out follow-up study.
The extraction of dichloro quinolinic acid and liquid chromatographic detection condition: take 1ml degradation solution to 2ml from
In heart pipe, add 0.25ml chloroform-n-butyl alcohol mixed liquor (volume ratio 4:1) deproteinization, fully
After vibration 30min, 12000rpm is centrifuged 5min, takes after upper strata aqueous phase crosses 0.22 μm aqueous phase filter membrane
Use liquid chromatographic detection.Liquid phase chromatogram condition: use Athana C18Post, flowing is first mutually
Alcohol: 0.2% acetic acid aqueous solution (volume ratio 60:40), column temperature 30 DEG C, flow velocity 0.8ml/min,
Detection wavelength 240nm, sample size 20 μ L.External standard method is pressed peak area quantification and is measured in sample two
The content of chloro-quinolinic acid, according to formula dichloro quinolinic acid degradation rate (%)=(blank group dichloro
Quinolinic acid concentration-degradation bacteria process group dichloroquinoline acid concentration)/blank group dichloroquinoline acid concentration
× 100% obtains its degradation rate.
3, the qualification of bacterial strain Q3
Q3 morphological characteristic: Q3 bacterium colony in LB culture medium in faint yellow, circular, surface light
Sliding, opaque.Scanning electron microscope hypothallus is rod-short, single or in short chain arrange, 1.2～
1.5 × 2.0～4.0 microns (see Fig. 2).
Biolog identifies: use Biolog Gen III microwell plate to identify for examination antibacterial,
4-6h culture identification result SIM value >=0.75 is acceptable result；After 16-24h cultivates
When reading result, SIM value >=0.5 is acceptable result.Cultivate through 18h, through instrument
Automatically scan reading and inquire about antibacterial data base, with SIM value >=0.5 as standard, Q3 being reflected
Being set to bacillus megaterium (Bacillus megaterium), its SIM value is 0.673, PROB
Value is 0.977, and DIST value is 5.774.
Molecular Identification: using test kit to extract degradation bacteria DNA, amplimer used is by Hua Da
Genome company synthesizes:
Forward primer is 5 '-AGAGTTTGATCMTGGCTCAG-3 '；
Downstream primer is 5 '-TACGGCTACCTTGTTACGACTT-3 '.
Amplification reaction system is: 10 × Buffer (Mg2+) 4 μ l, dNTPs3 μ l, each 1 μ l of primer,
Thallus DNA 2 μ l, Taq archaeal dna polymerase 1 μ l, add deionized water to 50 μ l.
PCR reaction condition: 94 DEG C of 5min, 94 DEG C of 30s, 55 DEG C of 40s, 72 DEG C of 60s；40
Individual circulation；Finally preserve 10min in 72 DEG C.
PCR primer uses agarose gel DNA recovery test kit to carry out purpose fragment recovery, returns
Receive product examining order to be completed by Hua Da genome company.Sequencing result enters GenBank data base
Compare.The 16S rDNA sequence of Q3 2377bp altogether is logged on GenBank compare
And phylogenetic tree construction (seeing Fig. 3), with bacillus megaterium (Bacillus megaterium)
(N1564-A29) homology is 100%, and the result identified with Biolog is coincide, and therefore will
This degradation bacteria Q3 is accredited as bacillus megaterium (Bacillus megaterium).
The degradation characteristic research of embodiment 2 degradation bacteria Q3
Medium pH is on the growth of degradation bacteria Q3 and the impact of degradation rate: prepares inorganic salt and cultivates
Base (adding trace sucrose and yeast powder as additive carbon) is sub-packed in triangular flask, often respectively
Bottled enter 50ml culture medium, regulation Medium's PH Value be respectively 5,6,7,8,9 (each places
Manage 3 repetitions), add dichloro quinolinic acid after sterilization treatment, make dichloroquinoline in each conical flask
The concentration of acid is 20mg/L, then accesses, with the inoculum concentration of 5% (volume fraction), the fall activated
Solve bacterium Q3 bacterium solution, put into 30 DEG C, 180r min-1Constant-temperature table in shaken cultivation.Take after 7d
Sample measures its OD600Value and degradation rate.
Cultivation temperature is on the growth of degradation bacteria Q3 and the impact of degradation rate: prepares inorganic salt and cultivates
Base (adding trace sucrose and yeast powder as additive carbon) is sub-packed in triangular flask, pH respectively
It is adjusted to 8, adds dichloro quinolinic acid after sterilization treatment, make dichloro quinolinic acid in each conical flask
Concentration be 20mg/L, access the degradation bacteria activated with the inoculum concentration of 5% (volume fraction)
Q3 bacterium solution, be respectively placed in 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C (each
Process 3 repetitions) constant-temperature table in dark shaken cultivation.Its OD of sampling and measuring after 7d600
Value and degradation rate.
Inoculum concentration is on the growth of degradation bacteria Q3 and the impact of degradation rate: prepare minimal medium
(adding trace sucrose and yeast powder as additive carbon) is sub-packed in triangular flask, pH respectively
It is adjusted to 8, adds dichloro quinolinic acid after sterilization treatment, make dichloro quinolinic acid in each conical flask
Concentration be 20mg/L, respectively with 2%, 4%, 6%, 8%, 10% inoculum concentration (volume integral
Number) access the Q3 bacterium solution (each process 3 repetition) activated, it is placed in 30 DEG C, 180r min-1
Constant-temperature table in shaken cultivation.Its OD of sampling and measuring after 7d600Value and degradation rate.
Dichloro quinolinic acid initial concentration is on the growth of degradation bacteria Q3 and the impact of degradation rate: prepare
Minimal medium (adding trace sucrose and yeast powder as additive carbon) is sub-packed in three respectively
In the bottle of angle, pH regulator is 8, adds dichloro quinolinic acid, make in each conical flask after sterilization treatment
The concentration of dichloro quinolinic acid is respectively 5,10,20,40,80mg/L, with 6% (volume fraction)
Inoculum concentration access the degradation bacteria Q3 bacterium solution that activated, be placed in 30 DEG C, 180r min-1Constant temperature
Shaken cultivation in shaking table.Its OD of sampling and measuring after 7d600Value and degradation rate.
Incubation time is on the growth of degradation bacteria Q3 and the impact of degradation rate: prepares inorganic salt and cultivates
Base (adding trace sucrose and yeast powder as additive carbon) is sub-packed in triangular flask, pH respectively
It is adjusted to 8, adds dichloro quinolinic acid after sterilization treatment, make dichloro quinolinic acid in each conical flask
Concentration be 20mg/L, access the degradation bacteria activated with the inoculum concentration of 6% (volume fraction)
Q3 bacterium solution, is placed in 30 DEG C, 180r min-1Constant-temperature table in shaken cultivation.Respectively in inoculation
Rear its OD of 1d, 2d, 3d, 4d, 5d, 6d, 7d sampling and measuring600Value and degradation rate.
Experimental result shows, in dichloro quinolinic acid raw degradation bacteria Q3 cultivation temperature be 30 DEG C,
PH=8, inoculum concentration 6%, dichloro quinolinic acid initial mass concentration are performance under conditions of 20mg/L
Gone out optimal degradation effect, its degradation rate of dichloro quinolinic acid has been reached when 7d 90% with
On.
The embodiment 3 bacterial strain Q3 prevention effect to Nicotiana tabacum L. poisoning
With K326 for for examination Nicotiana tabacum L..The Q3 bacterial strain of preservation is transferred to the liquid LB of sterilizing
In culture medium, in 30 DEG C, 180r/min constant-temperature table dark shaken cultivation overnight.
Taking front stubble rice terrace uses dichloro quinolinic acid dosage to be the soil of recommended dose, air-dries powder
The basin alms bowl of footpath 18cm, high 20cm, every basin dress soil 3kg is loaded after broken.7d before tobacco seedlings is transplanted
With Q3 fermentation liquid, (Q3 is through overnight cultivation and fermentation, OD600=0.63, with every strain 10ml fermentation liquid
Basin alms bowl is uniformly applied after being watered 200ml dilution.) carry out soil treatment.It is blank right to be respectively provided with
According to group (use and remain soil cultivation without dichloro quinolinic acid, without poisoning), poisoning matched group (two
Chloro-quinolinic acid residual soil, is not added with degradation bacteria and processes) and (the every strain of Q3 fermentation liquid of process group
10ml is watered soil treatment after dilution), often process and 3 repetitions be set, respectively after transplanting 50d,
65d investigation the leaf length of each process, leaf width (the 4th leaf unfolded the most completely) and
Plant height, calculates leaf length, Ye Kuan, plant height suppression ratio, data acquisition Microsoft Excel
2003 software processes.
The long suppression ratio of leaf (%)=(blank leaf length-poisoning leaf length)/blank leaf is long
Leaf width suppression ratio (%)=(blank leaf width-poisoning leaf width)/blank leaf width
Plant height suppression ratio (%)=(blank plant height-poisoning plant height)/blank plant height
Table 1 Q3 repairing effect to Nicotiana tabacum L. dichloro quinolinic acid poisoning
Seeing table 1, bacterial strain Q3 can alleviate the dichloro quinolinic acid poisoning to Nicotiana tabacum L. significantly.
30d after transplanting, the leaf length of poisoning matched group (Quinclorac but no Q3), Ye Kuan
And plant height is the most significantly suppressed, its value be respectively blank 83.18%,
64.75%, 67.67%, and the leaf of bacterial strain Q3 biological restoration group (Quinclorac+Q3)
Grow, leaf is wide and plant height has then returned to the 89.49% of blank, 92.14% and 93.64%.
45d after transplanting, the leaf length of poisoning matched group (Quinclorac but no Q3), Ye Kuan
And plant height is suppressed more, only the 79.25% of blank, 40.09%and
74.51%, and the leaf length of bacterial strain Q3 biological restoration group (Quinclorac+Q3), Ye Kuan
And plant height has then returned to the 93.40% of blank, 91.70% and 92.29%, it is shown that
Good recovery effects to Nicotiana tabacum L. dichloro quinolinic acid poisoning, sees Fig. 4.
The preparation of embodiment 4 bacterial strain Q3 microecological microbial agent
Activated strains Q3, is seeded to LB liquid medium, 180r/min, 30 DEG C of calorstat trainings
Support 24h.
Wherein prepare different dosage forms according to different application aspect:
(1) liquid preparation: use 500mL triangle bottled 100ml LB culture medium, conventional sterilant,
After cooling again on superclean bench, directly wash lower inclined plane thalline with sterilized water and inoculate into fermentation triangle
In Ping, shake-flask culture 30 DEG C, 180r/min, cultivates 24h.Proceed to ferment tank, carefully at once
The preparation of bacterium fermentation tank culture medium, sterilizing, inoculate after cooling and ferment, 30 DEG C, 180r/min,
Cultivating 48h stuck fermentation, zymocyte liquid reaches every milliliter of bacterium solution and contains 109～1012Individual spore.Adopt
With plastic bottle or plastic bag packaging fermentation liquid.
(2) solid powder: with reference to the preparation of antibacterial Shake flask medium, use 500mL triangular flask
Dress 100ml LB culture medium, conventional sterilant is after cooling again on superclean bench, straight with sterilized water
Connect and wash lower inclined plane thalline and inoculate in fermentation triangular flask, shake-flask culture 30 DEG C, 200r/min, training
Support 24h.Proceed to ferment tank at once, the preparation of bacterial fermentation tank culture medium, sterilizing, cold
Inoculate the most afterwards and ferment, 30 DEG C, 180r/min, cultivate 48h stuck fermentation, be centrifuged fermentation liquid,
Obtaining thalline, be added thereto to charcoal powder absorption thalline, the every g of formulation of thalline number contains
1012～1014Individual spore, then pack with plastic bag.
The embodiment 5 Tiny ecosystem degradation bacterial agent prevention effect to Nicotiana tabacum L. poisoning
Use Tiny ecosystem degradation bacterial agent (liquid preparation) pouring root when tobacco transplant.Every 1 kilogram
Degradation bacterial agent mixes with 600-800 times of clear water, carries out root irrigation after tobacco transplant.Nicotiana tabacum L.
Group's phase, then carry out dispenser 1 time.The effect of this insecticide-applying way and be characterized as degrading tobacco rhizosphere
Dichloro quinolinic acid around, reduces the Nicotiana tabacum L. absorption to dichloro quinolinic acid, alleviates dichloro quinolinic acid
To poisoning, improving tobacco production, its yield reaches more than 90% without poisoning field, and poisoning field
It is only without poisoning field 30%～40%, sees Fig. 5.
Use Tiny ecosystem degradation bacterial agent (solid preparation) to mix with Nicotiana tabacum L. base manure to use.Nicotiana tabacum L.
Before transplanting, mix with Nicotiana tabacum L. base manure by 1.5 kilograms of degradation bacterial agents/mu dosage, execute to field.Should
The effect of insecticide-applying way and the dichloro quinolinic acid being characterized as around degrading tobacco rhizosphere, reduce Nicotiana tabacum L.
Absorption to dichloro quinolinic acid, alleviates dichloro quinolinic acid to poisoning, raising tobacco production, its product
Amount reaches without poisoning field more than 90%, and poisoning field is only without poisoning field 30%～40%.Examination
Verify bright, illustrate that described microbial inoculum can prevent and treat the dichloro quinolinic acid poisoning to Nicotiana tabacum L. well.
Although, used general explanation, detailed description of the invention and test, to this
Bright make detailed description, but on the basis of the present invention, it can have been made some modifications or improvements,
This will be apparent to those skilled in the art.Therefore, without departing from present invention spirit
On the basis of these modifications or improvements, belong to the scope of protection of present invention.
Claims (10)
1. bacillus megaterium (Bacillus megaterium.) Q3, deposit number is: CGMCC
No.8576。
2. contain the microbial inoculum of bacillus megaterium Q3 described in claim 1.
3. the method preparing microbial inoculum described in claim 2, it is characterised in that at LB
In culture medium ferment bacillus megaterium Q3, the fermentation liquid obtained is prepared as liquid preparation or
Solid preparation.
Method the most according to claim 3, described fermentation condition is: fermentation temperature is
30 DEG C, rotating speed is 180r/min, and fermentation time is 3 days.
5. the answering in degraded dichloro quinolinic acid of bacillus megaterium Q3 described in claim 1
With.
Application the most according to claim 5, it is characterised in that: described huge spore bar
Bacterium Q3 is for degrading the dichloro quinolinic acid in water body or soil.
Application the most according to claim 5, it is characterised in that: described huge spore bar
Bacterium Q3 application in repairing Nicotiana tabacum L. dichloro quinolinic acid poisoning.
8. according to the application described in claim 6 or 7, it is characterised in that by described huge
Bacillus cereus Q3 is prepared as microbial inoculum, is inoculated in water body, soil or base manure.
Application the most according to claim 7, it is characterised in that by described huge spore
Bacillus Q3 is prepared as liquid preparation, and every ml of formulation contains 109～1012Individual spore, every 1 is public
Jin liquid preparation mixes with 600-800 times of clear water, carries out root irrigation after tobacco transplant.
10. according to the application described in claim 6 or 7, it is characterised in that by described huge
Bacillus cereus Q3 is prepared as solid powder, and every gram of powder contains 1012～1014Individual spore, at cigarette
Before grass is transplanted, mix with base manure by 1.5～2.0 kilograms of solid powders/mu dosage, execute to field soil
Earth.
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| CN105940808A (en) * | 2016-05-19 | 2016-09-21 | 贵州省烟草公司毕节市公司 | Quinclorac herbicide-caused tobacco phytotoxicity restoration method |
| CN107384829B (en) * | 2017-08-24 | 2020-04-14 | 暨南大学 | Application of bacillus megatherium YJB3 in artificial wetland water PAEs pollution purification |
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