CN104115873A - Biological-compound-type trichoderma inhibitor and preparation method thereof - Google Patents
Biological-compound-type trichoderma inhibitor and preparation method thereof Download PDFInfo
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- CN104115873A CN104115873A CN201410350082.2A CN201410350082A CN104115873A CN 104115873 A CN104115873 A CN 104115873A CN 201410350082 A CN201410350082 A CN 201410350082A CN 104115873 A CN104115873 A CN 104115873A
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 48
- 238000000855 fermentation Methods 0.000 claims abstract description 25
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- 239000000706 filtrate Substances 0.000 claims abstract description 15
- 239000002023 wood Substances 0.000 claims description 71
- 239000012530 fluid Substances 0.000 claims description 62
- 241001655322 Streptomycetales Species 0.000 claims description 46
- 150000001875 compounds Chemical class 0.000 claims description 44
- 241000894006 Bacteria Species 0.000 claims description 36
- 239000007788 liquid Substances 0.000 claims description 33
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- 235000015097 nutrients Nutrition 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
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- 239000004323 potassium nitrate Substances 0.000 claims description 3
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Abstract
The invention belongs to the technical field of microorganisms used for preventing and controlling pollution caused by trichoderma edible fungi, and in particular relates to a biological-compound-type trichoderma inhibitor and a preparation method thereof. The biological-compound-type trichoderma inhibitor aims at solving the problems that the existing trichoderma preventing and controlling agent is poor in effect, and high in residual quantity and cost. According to the technical scheme, the biological-compound-type trichoderma inhibitor is obtained in a way that bacillus subtilis ACCC11088 and streptomyces jingyangensis ACCC40041 are respectively subjected to single-strain fermental cultivation, so that fermentation liquors are obtained, and then the two fermentation liquors are uniformly mixed in a weight ratio of (1-5) to (5-1), so that the biological-compound-type trichoderma inhibitor is obtained; or residual filtrates of the two fermentation liquors after thalli are filtered in a weight ratio of (1-5) to (5-1), so that the biological-compound-type trichoderma inhibitor is obtained. The biological-compound-type trichoderma inhibitor has the advantages of high curative ratio, environmental friendliness and innocuousness.
Description
Technical field
The invention belongs to the microbial technology field of the mould pollution of control edible fungus wood, particularly mould inhibitor of a kind of biological compound wood and preparation method thereof.
Background technology
The mould green mould causing of wood is threat maximum in Edible Fungi process, conventionally adopts Agro-chemicals control in production, once adopt impolitic pesticide control in the fruiting phase, will cause the residual of agricultural chemicals.And use for a long time Single chemistry medicine can make disease resistance strengthen, control efficiency reduces, and residual quantity is large, and health is constituted a threat to, and contaminated environment.Recent domestic exploitation replaces the plant extracts of chemical pesticide as pyroligneous liquor, plant fumigation matter, the mould harm of little seeds of a tung oil tree extract one class extraction of substance control wood, although fungistatic effect is better, above finished product manufacturing cost is high, productive rate is lower.Therefore utilize the wooden mould good application prospect that has of microbial control.Existing mould microbial strains and the antibiotic of control wood that be used at present, as bacillus subtilis, natamycin etc.Although microorganism is better to the mould inhibition of wood, thereby can suppress composite better inhibition, the not report of reaching of the mould microbial strains of wood.
Summary of the invention
The object of the invention is to solve the technical problem that control efficiency is low, residual quantity is large and manufacturing cost is high that the mould medicament of existing control wood exists, mould inhibitor of a kind of biological compound wood and preparation method thereof is provided.
For solving the problems of the technologies described above, the technical scheme that the present invention takes is:
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 1～5:5～1 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Or the ratio that after the thalline of two kinds of zymotic fluids is filtered, remaining filtrate is 1～5:5～1 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
The preparation method of the mould inhibitor of biological compound wood of the present invention comprises the steps:
(1) preparation of single strain fermentating liquid:
The preparation of bacillus subtilis ACCC11088 zymotic fluid: bacillus subtilis ACCC11088 is inoculated in liquid nutrient medium under aseptic condition, 30 DEG C, the cultivation of 180r/min condition bottom fermentation, microscopy 80% forms gemma and makes, wherein said liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 9～11g, beef extract 3～5g, sodium chloride 4.5～5.5g, PH is adjusted between 7.2～7.4;
The preparation of Jingyang streptomycete ACCC40041 zymotic fluid: Jingyang streptomycete ACCC40041 is inoculated in Gao Shi I liquid nutrient medium under aseptic condition, 28 DEG C, the cultivation of 180r/min condition bottom fermentation, fermentation is carried out making for 5～7 days, wherein said Gao Shi I medium is: the content of each component in 1.0L distilled water is: soluble starch: 18～23g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.4～0.6g, epsom salt 0.4～0.6g, sodium chloride 0.4～0.6g, ferrous sulfate heptahydrate 0.01g, PH is adjusted between 7.4～7.6;
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 1～5:5～1 according to weight ratio is mixed;
Or bacillus subtilis ACCC11088 zymotic fluid and Jingyang streptomycete ACCC40041 zymotic fluid are filtered respectively, get the ratio that filtrate is separately 1～5:5～1 according to weight ratio and be mixed.
The mould inhibitor of wood composite of the present invention is composited by two kinds of beneficial microbes, is respectively bacillus subtilis ACCC11088 and the Jingyang streptomycete ACCC40041 that preserve at China Committee for Culture Collection of Microorganisms agricultural microorganism center.They can effectively suppress the mould growth of edible fungus wood at hypha of edible fungus early growth period, can not suppress the growth of hypha of edible fungus simultaneously, and nontoxic to people and animals.
For further showing the effect of the mould inhibitor of biological compound wood of the present invention, the present invention has done following experiment:
One, from the planting material of flat mushroom, mushroom and Asparagus of morbidity, separate wooden mould fungi, for the mould antagonistic experiment of wood provides pathogen, concrete steps are as follows:
1, from flat mushroom, mushroom and golden mushroom plantation material or the fruit body of morbidity the mould spore of picking wood on PDA flat board, be placed in 26 DEG C of cultivations, observe cultured flat board, select growth population many, green bacterium colony carries out purifying, picking mycelia lines on PDA flat board, and 26 DEG C of cultivations, after obtaining pure bacterium colony, be inoculated in PDA slant tube, be stored in 4 DEG C of refrigerators; The Trichoderma of purifying in 26 DEG C of dark culturing, is observed morphological feature and the color of bacterium colony every day on PDA medium, measures colony diameter picking mycelia and spore film-making, in the form of micro-Microscopic observation conidiophore branching morphology, conidiogenous cell and spore;
2, through separation and purification, isolate a strain Trichoderma from mushroom and be numbered XG, isolate two strain Trichodermas from flat mushroom and be numbered M3, M5, isolates a strain Trichoderma from Asparagus and is numbered JZG, and being characterized as of each bacterial strain is as follows:
XG: on PDA flat board, bacterium colony is just white in color velvet-like, there is the closely knit Chan Bao of the mycelia of colyliform district in the later stage; color is green to bottle green; conidiophore is arranged in the form of a ring, and secondary branch is single or taking 2-3 as one group, wide-angle is stretched out; branch is bending or wavy; the contracting of hanging of bottle metulae portion, middle part is wider, and formation long-neck above narrows from middle part; conidium is spherical in shape, surperficial spinosity.
M3: mycelial growth is fast, the back side is colourless to faint yellow, bacterium colony is just white in color, after become green, the later stage has the obvious Chan Bao of circle district in the middle of the bacterium colony, conidiophore branch is many, crotch angle is large, and bottle stalk is elongated, and life, wheel are born on sporophore, end bottle stalk is longer, conidium ovalize or oval.
M5: bacterium colony is just white in color, after become green, there is the obvious Chan Bao of circle district in the later stage in the middle of the bacterium colony, conidiophore branch is many, crotch angle is large, bottle stalk is elongated, and life, wheel are born on sporophore, conidium is short cylinder.
JZG: mycelial growth is very fast, the colourless or dark yellow in the back side.Bacterium colony is just white in color velvet-like, after become greenly to bottle green, there is the closely knit Chan Bao of the mycelia of colyliform district in the later stage, conidiophore branch is few, secondary branch single or 2-3 be one group, wide-angle is stretched out, branch is slightly bending.
Two, edible mushroom infects wooden mould treatment experiment
Experimental technique:
1, the preparation of cultivation bag
By mushroom cultivation raw material cotton seed hull and corncob after simple fermentation process (about approximately 5 days), pack, and inoculate flat mushroom strain, for subsequent use in the time that Pleurotus ostreatus grows to 3～5cm left and right.
2, the preparation of various antagonistic strains
Prepare respectively common, Gao Shi liquid nutrient medium, at 28 DEG C, shaking flask is cultivated, and bacterium liquid has been cultivated rear for subsequent use.
3, pathogen returns and connects
By after M5, M3, XG and the activation of JZG pathogen, draw quantitative being injected in bacterium bag of 0.1ml with liquid-transfering gun, cultivate for subsequent use after 2～3 days.
4, pathogen treatment
In the time that pathogen grows into 3～8cm, the bacterium liquid of drawing bacillus subtilis ACCC11088+ Jingyang streptomycete ACCC40041 stoste, 10 times of dilutions and filtered fluid with syringe injects pathogen position, ensure that Antagonistic Fungi liquid touches pathogen, measures the cure rate of each processing.
Experimental result: stoste processed group reaches 88%, 93%, 90%, 91% to four mould pathogen cure rates of wood; Ten times of dilution processed group reach 75%, 81%, 84%, 84% to four mould pathogen cure rates of wood; Thalline filtered fluid processed group reaches 85%, 91%, 90%, 91% to four mould pathogen cure rates of wood; The cure rate of blank is 5%, 5%, 10%, 9%.Concrete data are in table 1.
Above-mentioned experiment shows, the present invention reaches 85%～91% to the mould inhibiting rate of the wood of flat mushroom, mushroom, Asparagus.
The result for the treatment of of table 1 compounded combination to XG, JZG, M3 and M5 pathogen
Embodiment
Embodiment 1
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 1:1 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The preparation of bacillus subtilis ACCC11088 zymotic fluid: bacillus subtilis ACCC11088 is inoculated in common liq medium under aseptic condition, described liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 10g, beef extract 3g, sodium chloride 5g, PH is adjusted between 7.2～7.4,30 DEG C, the cultivation of 180r/min condition bottom fermentation, microscopy 80% forms gemma.
The preparation of Jingyang streptomycete ACCC40041 zymotic fluid: Jingyang streptomycete ACCC40041 is inoculated in Gao Shi I liquid nutrient medium under aseptic condition, described Gao Shi I medium is: the content of each component in 1.0L distilled water is: soluble starch: 20g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, epsom salt 0.5g, sodium chloride 0.5g, ferrous sulfate heptahydrate 0.01g, PH is adjusted between 7.4～7.6,28 DEG C, the cultivation of 180r/min condition bottom fermentation, and fermentation is carried out 5～7 days.
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 1:1 according to weight ratio is mixed.
Embodiment 2
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 1:3 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 1:3 according to weight ratio is mixed.
Embodiment 3
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 1:5 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 1:5 according to weight ratio is mixed.
Embodiment 4
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 2:1 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 2:1 according to weight ratio is mixed.
Embodiment 5
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 5:1 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 5:1 according to weight ratio is mixed.
Embodiment 6
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that after then the thalline of two kinds of zymotic fluids being filtered, remaining filtrate is 1:1 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
After the thalline of the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid filters, filtrate is that 1:1 ratio is mixed according to weight ratio.
Embodiment 7
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that after then the thalline of two kinds of zymotic fluids being filtered, remaining filtrate is 1:2 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
After the thalline of the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid filters, filtrate is that 1:2 ratio is mixed according to weight ratio.
Embodiment 8
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that after then the thalline of two kinds of zymotic fluids being filtered, remaining filtrate is 1:4 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
After the thalline of the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid filters, filtrate is that 1:4 ratio is mixed according to weight ratio.
Embodiment 9
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that after then the thalline of two kinds of zymotic fluids being filtered, remaining filtrate is 3:1 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
After the thalline of the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid filters, filtrate is that 3:1 ratio is mixed according to weight ratio.
Embodiment 10
The mould inhibitor of a kind of biological compound wood, it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that after then the thalline of two kinds of zymotic fluids being filtered, remaining filtrate is 5:1 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
Concrete preparation method comprises the steps:
(1) preparation of single strain fermentating liquid:
The method that adopts the present embodiment 1, makes single strain fermentating liquid.
(2) preparation of the mould inhibitor of wood composite:
After the thalline of the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid filters, filtrate is that 5:1 ratio is mixed according to weight ratio.
Claims (2)
1. the mould inhibitor of biological compound wood, it is characterized in that: it is to carry out respectively single strain fermentation cultivation by bacillus subtilis ACCC11088 and Jingyang streptomycete ACCC40041 to make zymotic fluid, and the ratio that is then 1～5:5～1 by two kinds of zymotic fluids according to weight ratio mixes makes the mould inhibitor of biological compound wood; Or the ratio that after the thalline of two kinds of zymotic fluids is filtered, remaining filtrate is 1～5:5～1 according to weight ratio mixes makes the mould inhibitor of biological compound wood; Wherein in the mould inhibitor of biological compound wood, the bacterium number of bacillus subtilis ACCC11088 is 0.5 hundred million/mL～4,000,000,000/mL, and the bacterium number of Jingyang streptomycete ACCC40041 is 0.5 hundred million/mL～2,000,000,000/mL.
2. the preparation method of the mould inhibitor of biological compound wood claimed in claim 1, is characterized in that: comprise the steps:
(1) preparation of single strain fermentating liquid:
The preparation of bacillus subtilis ACCC11088 zymotic fluid: bacillus subtilis ACCC11088 is inoculated in liquid nutrient medium under aseptic condition, 30 DEG C, the cultivation of 180r/min condition bottom fermentation, microscopy 80% forms gemma and makes, wherein said liquid nutrient medium is: the content of each component in 1.0L distilled water is: peptone 9～11g, beef extract 3～5g, sodium chloride 4.5～5.5g, PH is adjusted between 7.2～7.4;
The preparation of Jingyang streptomycete ACCC40041 zymotic fluid: Jingyang streptomycete ACCC40041 is inoculated in Gao Shi I liquid nutrient medium under aseptic condition, 28 DEG C, the cultivation of 180r/min condition bottom fermentation, fermentation is carried out making for 5～7 days, wherein said Gao Shi I medium is: the content of each component in 1.0L distilled water is: soluble starch: 18～23g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.4～0.6g, epsom salt 0.4～0.6g, sodium chloride 0.4～0.6g, ferrous sulfate heptahydrate 0.01g, PH is adjusted between 7.4～7.6;
(2) preparation of the mould inhibitor of wood composite:
The ratio that the bacillus subtilis ACCC11088 zymotic fluid that step (1) is prepared and Jingyang streptomycete ACCC40041 zymotic fluid are 1～5:5～1 according to weight ratio is mixed;
Or bacillus subtilis ACCC11088 zymotic fluid and Jingyang streptomycete ACCC40041 zymotic fluid are filtered respectively, get the ratio that filtrate is separately 1～5:5～1 according to weight ratio and be mixed.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494443A (en) * | 2016-01-21 | 2016-04-20 | 唐睿 | Compound microorganism agent and application thereof |
| CN106212505A (en) * | 2016-07-27 | 2016-12-14 | 吉林农业科技学院 | Bacillus pumilus purposes in preventing and treating Ganoderma Trichoderma atroviride disease |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401587A (en) * | 2008-11-21 | 2009-04-08 | 天津科技大学 | Bacillus subtilis formulation for preventing and controlling soil-borne disease and method of preparing the same |
| CN103798087A (en) * | 2014-03-04 | 2014-05-21 | 黑龙江省科学院微生物研究所 | Trichoderma disease prevention method for black fungus |
-
2014
- 2014-07-22 CN CN201410350082.2A patent/CN104115873A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401587A (en) * | 2008-11-21 | 2009-04-08 | 天津科技大学 | Bacillus subtilis formulation for preventing and controlling soil-borne disease and method of preparing the same |
| CN103798087A (en) * | 2014-03-04 | 2014-05-21 | 黑龙江省科学院微生物研究所 | Trichoderma disease prevention method for black fungus |
Non-Patent Citations (1)
| Title |
|---|
| 张雯: ""泾阳链霉菌代谢产物抑菌性能研究及发酵工艺优化"", 《中国酿造》, no. 14, 30 July 2008 (2008-07-30), pages 32 - 34 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494443A (en) * | 2016-01-21 | 2016-04-20 | 唐睿 | Compound microorganism agent and application thereof |
| CN106212505A (en) * | 2016-07-27 | 2016-12-14 | 吉林农业科技学院 | Bacillus pumilus purposes in preventing and treating Ganoderma Trichoderma atroviride disease |
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