CN104107733B - A kind of reagent bottle and reagents loaded device and method thereof - Google Patents

Abstract

The invention discloses a reagent bottle, a reagent loading device and a method thereof. The reagent bottle comprises a bottle body, a bottle cap and a bottle stopper. The bottle cap is covered on the top of the bottle body. The plug is plugged at the through hole. The reagent loading device includes a bracket and a puncture needle; the bracket is used to support and guide the reagent bottle; the puncture needle includes a needle point, a needle seat and a needle tail thread that are fixedly connected in sequence. When loading the reagent, just unscrew the bottle cap, press down the reagent bottle directly, so that the puncture needle passes through the bottle stopper at the bottom, and the loading of the reagent bottle can be completed. The bottom puncture method of the present invention is used to load the reagent, which is fast and convenient, and does not need both hands to cooperate at the same time. When the reagent is unloaded, just pull the reagent bottle away from the puncture needle, which will not cause the reagent to drip; when the reagent is used, unscrew it The bottle cap can directly pour reagents from the mouth of the bottle, which can replenish reagents in real time, which solves the shortcomings of the existing technology that cannot be replenished in real time or is inconvenient to replenish.

Description

Reagent bottle and its reagent loading device and method

technical field

The invention relates to the field of medical equipment, in particular to a reagent bottle and a reagent loading device and method thereof.

Background technique

In the field of automatic analysis of biochemical analysis and immune analysis, certain reagents are used, and the reagents need to be dispensed using an independent liquid circuit system, that is, the inlet end of the liquid circuit is connected to the reagent bottle, and the reagent is sucked from the reagent bottle. Reagent, the outlet end of the liquid path is connected to a device such as a reaction vessel or a measurement chamber to realize dispensing of the reagent. These reagents are generally used for multiple test items, such as cleaning solutions in biochemical analysis, used to clean instrument fluid lines, sampling needles, and reaction cups; cleaning buffers in immunoassays, used for heterogeneous chemiluminescence immunoassays Cleaning of unbound substances in analysis B/F (Bound-Free, cleaning and separation); pre-excitation solution, excitation solution or luminescence substrate solution in immunoassay, used for luminescence after heterogeneous chemiluminescence immunoassay B/F Reaction; reagents such as diluent and hemolytic agent in blood cell analysis, used for the detection of blood cells. Such reagents are generally filled in general-purpose reagent bottles (or reagent buckets, hereinafter collectively referred to as reagent bottles). When the old reagent bottles on the instrument are used up, they need to be unloaded first and then loaded with new reagent bottles.

In the prior art, the reagent bottle adopts a common structure, such as a square or cylindrical shape. When loading it on the loading position on the instrument, it is necessary to unscrew the bottle cap on the new reagent bottle and throw it away, and then put it on the loading position. Unload the old reagent bottle and replace it with a new reagent bottle. In the process of unloading the old reagent bottle, it is necessary to manually unscrew the bottle cap connected to the suction tube (some bottle caps are also connected to liquid level sensors such as liquid level floats), and lift the bottle cap up with one hand until the suction tube Remove the liquid tube from the mouth of the bottle, remove the old reagent bottle with the other hand, then put the suction tube into the new reagent bottle and screw on the bottle cap. The bottle cap connected with the pipette is different from the new bottle cap on the new reagent bottle. It adopts a special design. In order to maintain the pressure in the bottle, there is usually a vent part on the bottle cap or the bottom of the bottle or a vent tube is connected. road. In addition, in the existing ventilation method at the bottom of the bottle, after the air enters the bottle, it will pass through the reagent and float to the reagent liquid surface, especially when there are surfactants and other components in the reagent, many particles will be formed on the reagent liquid surface. Air bubbles or foam will adversely affect the performance of the reagent.

The structure of this general-purpose reagent bottle is complicated, and liquid level sensors such as reagent bottle caps and special liquid level floats used on the instrument need to be specially designed, as well as related ventilation devices, and the cost of use is high; the reagent loading operation requires the user to cooperate with both hands. In the process, the reagents adsorbed on the pipette and the float are easy to drip, and the dripping reagents have potential biological risks and are likely to cause harm to people.

Contents of the invention

The purpose of the present invention is to aim at the above-mentioned defect in the prior art, design a kind of fast loading, convenient and safe reagent bottle and its reagent loading device and method thereof, solve existing reagent bottle and reagent loading device existing in the reagent loading process Problems such as inconvenient operation, reagent dripping, health hazards, and inability to replenish reagents in real time.

To achieve the above object, the present invention adopts the following technical solutions:

A reagent bottle, comprising a bottle body, a bottle cap and a bottle stopper, the bottle cap is covered on the top of the bottle body, the bottom of the bottle body has a through hole communicating with the inside of the bottle body, and the bottle stopper is plugged in the through hole hole;

Wherein, the bottom of the bottle body has a concave hole concave to the inside of the bottle body, and the bottom end surface of the concave hole has a flange, and the flange forms a through hole;

The bottle stopper comprises a plug body, a neck in the middle and an undercut, the undercut and the plug body sandwich the neck, the diameter of the undercut is larger than the through hole, and the size of the neck is the same as that of the through hole Matching, the diameter of the plug body is larger than the through hole;

The axial thickness of the neck of the bottle stopper matches the thickness of the flange, so that the flange is clamped between the undercut and the plug body.

A reagent loading device, used to load the above reagent bottle, comprising a bracket and a puncture needle; the bracket is used to support and guide the reagent bottle; the puncture needle includes a needle point, a needle seat and a needle tail thread connected in sequence, There is a through hole in the center of the needle, the through hole forms a long and narrow opening on the oblique side of the needle tip, the needle seat is connected to the bracket, and the needle tail thread is connected to the external infusion pipeline;

The bracket includes a guide groove, the puncture needle is installed at the bottom of the guide groove, and the guide groove is used to guide the reagent bottle so that the bottle stopper on the reagent bottle is opposite to the puncture needle;

The needle seat is detachably connected to the bracket, wherein, the bottom surface of the guide groove has two arc-shaped fixing pieces, and the needle seat of the puncture needle has two correspondingly arranged clamping parts, and the puncture needle is screwed in and out by screwing in and out. The clamping part is screwed in or out of the fixing piece for fixing and loosening.

An immune analyzer, which is provided with the reagent loading device.

A method for loading the reagent bottle using the reagent loading device, comprising:

Align the stopper of the reagent bottle with the puncture needle of the reagent loading device;

Push the reagent bottle toward the needle tip so that the needle tip passes through the bottle stopper and enters the reagent bottle.

The invention provides a reagent bottle and its reagent loading device and method. The through hole at the bottom of the reagent bottle is plugged into the bottle stopper, and a puncture needle is installed on the reagent loading position of the reagent loading device; Bottle cap, directly press down the reagent bottle so that the puncture needle passes through the bottle stopper at the bottom to complete the loading of the reagent bottle. The bottom puncture method of the present invention is used to load the reagent, which is fast and convenient, and does not need both hands to cooperate at the same time. When the reagent is unloaded, just pull the reagent bottle away from the puncture needle, which will not cause the reagent to drip; when the reagent is used, unscrew it The bottle cap can directly pour reagents from the mouth of the bottle, which can replenish reagents in real time, which solves the shortcomings of the existing technology that cannot be replenished in real time or is inconvenient to replenish. On the other hand, the unscrewable bottle cap can not only prevent dust and other pollutants from entering the bottle, but also allow the air outside the bottle to enter the bottle when the reagent is used, so as to maintain the air pressure balance in the bottle, eliminating the need for specially designed The air intake device saves the cost. In addition, adopting the method of liquid outlet at the bottom and air intake at the top can effectively solve the adverse effect of the prior art on the performance of the reagent caused by the air intake at the bottom.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. Those skilled in the art can also obtain other drawings based on these drawings without creative work.

Fig. 1 is a schematic diagram of the assembled structure of the reagent bottle according to the first embodiment of the present invention.

Fig. 2 is a schematic diagram of the split structure of the reagent bottle according to the first embodiment of the present invention.

Fig. 3 is a schematic structural view of the bottle stopper in Embodiment 1 of the present invention.

Fig. 4 is a schematic diagram of an improved structure of the bottle stopper in Embodiment 1 of the present invention.

Fig. 5 is a schematic diagram of the overall structure of the reagent bottle according to the second embodiment of the present invention.

Fig. 6 is a schematic cross-sectional structure diagram of a reagent bottle according to Example 2 of the present invention.

FIG. 7 is a partial perspective cross-sectional schematic diagram of reagent bottle loading performed by the reagent loading device according to Embodiment 3 of the present invention.

Fig. 8 is a schematic structural view of the puncture needle in the third embodiment of the present invention.

FIG. 9 is a schematic diagram of the installation structure of the needle holder in the third embodiment of the present invention.

detailed description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Embodiment one

As shown in FIG. 1 and FIG. 2 , a reagent bottle provided by an embodiment of the present invention consists of a bottle cap 3 , a bottle body 1 and a bottle stopper 2 .

The outer surface of the top bottleneck of the bottle body 1 has an external thread 11, the lower inner surface of the bottle cap 3 has an internal thread, and the bottle cap 3 cooperates with the external thread 11 through the internal thread to cover the top of the bottle body 1. . The bottom of the bottle body 1 has a concave hole 12 which is concave to the inside of the bottle body 1 . The bottom end surface of the concave hole 12 has a flange 13 and a through hole 14 is formed by the flange 13 .

As shown in Figure 3, the bottle stopper 2 includes an undercut 21, a neck 22 and a plug body 23, the undercut 21 is located at the top of the neck 22, the plug body 23 is located at the bottom of the neck 22, the undercut 21 and the plug body 23 clamps the neck 22 in the middle, the size of the neck 22 matches the through hole 14, and the diameter of the undercut 21 and the plug body 23 is larger than the through hole 14.

Specifically, the material of the bottle stopper 2 can be general or special rubber, such as butyl rubber, ethylene propylene rubber or silica gel. The axial thickness of the neck 22 of the cork 2 (that is, the distance between the undercut 21 and the plug body 23 ) matches the thickness of the flange 13 , so that the flange 13 is clamped between the undercut 21 and the plug body 23 . Specifically, after the undercut 21 of the bottle stopper 2 passes through the through hole 14 at the bottom of the bottle body 1 through extrusion and deformation, the undercut 21 resumes its deformation, and the periphery fits on the surface of the bottle bottom flange 13 facing the inside of the bottle body 1 , and fastened on it, can prevent the bottle cork 2 from leaving the bottle body 1 outward; Disengage from bottle body 1 inside.

After the bottle stopper 2 is inserted into the bottle body 1 from the through hole 14, the neck 22 of the bottle stopper 2 cooperates with the through hole 14, and the stopper body 23 cooperates with the concave hole 12 at the bottom of the bottle. In order to achieve sealing and further prevent the bottle stopper 2 from falling off, at least one of the above two fits must be an interference fit; in order to facilitate assembly, one of them is preferably a non-interference fit. In the embodiment of the present invention, preferably, the fit between the neck portion 22 and the through hole 14 is a non-interference fit, and the fit between the plug body 23 and the concave hole 12 is an interference fit.

As an improvement, the bottom of the bottle body 1 in the embodiment of the present invention is in an inverted conical shape, and the inverted conical structure of the bottom of the bottle can effectively reduce the dead volume in the bottle body 1 (during normal testing, in order to prevent the pipeline from entering air bubbles, the inside of the bottle Generally, there will be residual reagents, and the residual reagent volume in the bottle is the dead volume). Furthermore, the top of the undercut 21 of the bottle stopper 2 is provided with a notch 210 , which can further reduce the dead volume in the bottle body 1 .

Another improved structure of the bottle stopper 2 in the embodiment of the present invention is shown in FIG. 4 , in which a concave ring 230 is provided on the side of the plug body 23 of the bottle stopper 2 shown in the figure, and other parts of the structure are the same as those shown in FIG. 3 . This concave ring structure makes the plug body 23 more easily deformed, and when the plug body 23 is in an interference fit with the concave hole 12, it is easier to plug into the bottle body 1 .

Embodiment two

As shown in FIG. 5 and FIG. 6 , another reagent bottle provided by the embodiment of the present invention is composed of a bottle cap 3 , a bottle body 1 , a bottle stopper fixing member 4 and a bottle stopper 2 . Wherein, the bottle cap 3 is covered on the top of the bottle body 1; the bottom of the bottle body 1 has a bottle mouth protruding to the outside of the bottle body, and the bottle stopper 2 is plugged in the through hole of the bottle mouth; the bottle stopper 4 It is also a bottle cap, which is placed on the bottleneck at the bottom of the bottle body 1, and simultaneously the bottle stopper 2 is pressed tightly on the through hole of the bottleneck.

Because the bottle cap is the most commonly used and the easiest part to combine with the bottle body, the embodiment of the present invention uses the bottle cap as the bottle stopper 4. In addition, other common fixing structures such as aluminum seals can also be used, as long as the The bottle stopper 2 is pressed tightly on the through hole, so that the function of preventing the bottle stopper 2 from falling off can be realized.

Embodiment Three

A reagent loading device provided by an embodiment of the present invention is shown in FIG. 7 and consists of a bracket 20 and a puncture needle 30 . The bracket 20 is used to support and guide the reagent bottle 10 .

Specifically, the bracket 20 is provided with a guide groove 21, and the puncture needle 30 is installed at the bottom of the guide groove 21; the guide groove 21 is used to guide the reagent bottle 10 after it is placed in the loading position, so that the reagent bottle 10 The center of the bottle stopper 2 is automatically aligned with the puncture needle 30 at the bottom of the guide groove 21 .

As shown in FIG. 8 , the puncture needle 30 includes a needle point 31 , a needle seat 33 and a needle tail thread 35 fixedly connected in sequence, and a through hole 34 is opened in the center of the puncture needle 30 , and the through hole 34 is formed on the oblique side of the needle point 31 Elongated opening 32 . The opening height of the elongated opening 32 needs to ensure that at least a part of the elongated opening 32 is exposed outside the cork 2 after the needle tip 31 passes through the cork 2 . The needle seat 33 is used to fix the puncture needle 30 on the bracket 20 . The needle tail thread 35 is used to connect with the external infusion pipeline through a sealed joint to form a liquid system for dispensing reagents. The external pipeline is connected with liquid circuit control devices such as syringe pumps and valves, and is used to control the reagents in the reagent bottle 10. raise.

For the convenience of maintenance and replacement, the puncture needle 30 in the embodiment of the present invention is detachably fixed to the bottom of the guide groove 21 through the needle holder 33 . Specifically, the puncture needle 30 can be fixed and loosened by simply screwing in and out; as shown in FIG. A correspondingly set holding part. When fixing, it is only necessary to rotate the puncture needle 30 by 90 degrees and screw the clamping part into the fixing piece 22 .

As an improvement, indicator lights or buttons corresponding to the reagent bottles 10 can also be installed on the bracket 20 . The indicator lights are used to display the usage status of the reagent in the reagent bottle 10 in real time, such as "full", "in use", "insufficient remaining" and "exhausted". The buttons are used for human-computer interaction, that is, when the operator replaces the reagent bottle 10 or replenishes the reagent, he presses the corresponding button to notify the instrument to update the status display of the reagent bottle 10 .

Correspondingly, the present invention also provides a method for loading the reagent bottle using the above reagent loading device, which specifically includes the following steps:

S1, unscrew the bottle cap 3;

S2, through the guide groove 21, the bottle stopper 2 of the reagent bottle 10 is opposite to the puncture needle 30 of the reagent loading device;

S3. Push the reagent bottle 10 in the direction of the needle point 31, so that the needle point 31 passes through the bottle stopper 2 and enters the reagent bottle 10, and the loading is completed.

It is very convenient to use the reagent bottle and the reagent loading device of the present invention to load the reagent, and the reagent will not drip out during the loading process, which avoids the potential danger and injury of the reagent to the operator. During the use of reagents, the operator can also uncover the bottle cap at any time to observe the use of reagents in the bottle. When the reagent volume is found to be insufficient, the reagent can be directly poured in to realize real-time replenishment of consumables, and the operation is convenient and reliable.

The invention provides a reagent bottle and its reagent loading device and method. The through hole at the bottom of the reagent bottle is plugged into the bottle stopper, and a puncture needle is installed on the reagent loading position of the reagent loading device; Bottle cap, directly press down the reagent bottle so that the puncture needle passes through the bottle stopper at the bottom to complete the loading of the reagent bottle. The bottom puncture method of the present invention is used to load the reagent, which is fast and convenient, and does not need both hands to cooperate at the same time. When the reagent is unloaded, just pull the reagent bottle away from the puncture needle, which will not cause the reagent to drip; when the reagent is used, unscrew it The bottle cap can directly pour reagents from the mouth of the bottle, which can replenish reagents in real time, which solves the shortcomings of the existing technology that cannot be replenished in real time or is inconvenient to replenish. On the other hand, the unscrewable bottle cap can not only prevent dust and other pollutants from entering the bottle, but also allow the air outside the bottle to enter the bottle when the reagent is used, so as to maintain the air pressure balance in the bottle, eliminating the need for specially designed The air intake device saves the cost. In addition, adopting the method of liquid outlet at the bottom and air intake at the top can effectively solve the adverse effect of the prior art on the performance of the reagent caused by the air intake at the bottom.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be Modifications are made to the technical solutions described in the foregoing embodiments, or equivalent replacements are made to some of the technical features; these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims (12)

1. A reagent bottle, characterized in that it comprises a bottle body, a bottle cap and a bottle stopper, the bottle cap is covered on the top of the bottle body, the bottom of the bottle body has a through hole communicating with the inside of the bottle body, the bottle body plugging in the through hole; Wherein, the bottom of the bottle body has a concave hole concave to the inside of the bottle body, and the bottom end surface of the concave hole has a flange, and the flange forms a through hole; The bottle stopper includes a plug body, a neck in the middle and an undercut, the undercut and the plug body sandwich the neck, the diameter of the undercut is larger than the through hole, and the size of the neck is the same as that of the through hole Matching, the diameter of the plug body is larger than the through hole; The axial thickness of the neck of the bottle stopper matches the thickness of the flange, so that the flange is clamped between the undercut and the plug body. 2 . The reagent bottle according to claim 1 , further comprising a bottle stopper fixing member connected to the bottle body to press the bottle stopper tightly to the through hole. 3 . 3. The reagent bottle according to claim 1, wherein the bottle stopper is inserted through the through hole, the neck of the bottle stopper cooperates with the through hole, the plug body cooperates with the concave hole, and at least One is an interference fit. 4. The reagent bottle according to claim 3, characterized in that the plug body is in an interference fit with the concave hole. 5. The reagent bottle according to claim 4, characterized in that, the side of the plug body is provided with a concave ring. 6 . The reagent bottle according to claim 5 , wherein a notch is provided on the top of the undercut. 7 . 7. A reagent loading device, for loading the reagent bottle according to claim 1, characterized in that it comprises a support and a puncture needle; the support is used to support and guide the reagent bottle; the puncture needle includes a sequential connection The needle point, needle seat and needle tail thread, the center of the puncture needle has a through hole, the through hole forms a narrow and long opening on the oblique side of the needle point, the needle seat is connected to the bracket, and the needle tail thread is connected to the external infusion line connect; The bracket includes a guide groove, the puncture needle is installed at the bottom of the guide groove, and the guide groove is used to guide the reagent bottle so that the bottle stopper on the reagent bottle is opposite to the puncture needle; The needle seat is detachably connected to the bracket, wherein, the bottom surface of the guide groove has two arc-shaped fixing pieces, and the needle seat of the puncture needle has two correspondingly arranged clamping parts, and the puncture needle is screwed in and out by screwing in and out. The clamping part is screwed in or out of the fixing piece for fixing and loosening. 8 . The reagent loading device according to claim 7 , wherein at least a part of the elongated opening is exposed outside the bottle stopper after the needle tip passes through the bottle stopper. 9. The reagent loading device according to claim 7, further comprising an indicator light or a button installed on the bracket for displaying or updating the usage status of the reagent in the reagent bottle. 10. An immune analyzer, characterized in that the reagent loading device according to any one of claims 7 to 9 is provided on the immune analyzer. 11. A method of using the reagent loading device according to claim 7 to load the reagent bottle according to claim 1, characterized in that, comprising: Align the stopper of the reagent bottle with the puncture needle of the reagent loading device; Push the reagent bottle toward the needle tip so that the needle tip passes through the bottle stopper and enters the reagent bottle. 12. The method according to claim 11, further comprising: loosening the bottle cap.