CN104095186A - Preparation method for probiotic sustained release agent containing plant extracts - Google Patents

Preparation method for probiotic sustained release agent containing plant extracts Download PDF

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Publication number
CN104095186A
CN104095186A CN201410344986.4A CN201410344986A CN104095186A CN 104095186 A CN104095186 A CN 104095186A CN 201410344986 A CN201410344986 A CN 201410344986A CN 104095186 A CN104095186 A CN 104095186A
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China
Prior art keywords
sustained release
preparation
release agent
probio
plant extracts
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Pending
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CN201410344986.4A
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Chinese (zh)
Inventor
陈颖
杨丽维
张峻
陈晓云
齐欣
唐柳
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Tianjin Forestry and Fruit Tree Institute
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Tianjin Forestry and Fruit Tree Institute
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Priority to CN201410344986.4A priority Critical patent/CN104095186A/en
Publication of CN104095186A publication Critical patent/CN104095186A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The present invention discloses a preparation method for probiotic sustained release agent containing plant extracts. The preparation method comprises: (1) dissolving grape polyphenol powder and edible fungi polysaccharide powder in a mixed liquor of complex probiotics to get stock solution A; (2) homogeneously mixing the stock solution A with alginate at a certain ratio and adding acetic acid to conducting fully swelling so as to get a stock solution B; (3) the stock solution B was added dropwisely to a solution of calcium chloride with a syringe to obtain a sustained release gel microspheres; (4) collecting the sustained release gel microspheres and drying at room temperature to get the probiotic sustained release agent containing grape polyphenols and edible fungi polysaccharides. With the synergic effect of grape polyphenols, edible fungi polysaccharides and probiotics, the present invention not only can improve the effectiveness of the active probiotics, but also strengthen the body's immune system and prevent various diseases.

Description

A kind of preparation method of the probio sustained release agent containing plant extracts
Technical field
The present invention relates to processing of farm products field, be specifically related to a kind of preparation method of the probio sustained release agent containing plant extracts.
Background technology
Probio (Probiotics) is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system, thereby can produce the active beneficial microorganism general name that definite health efficacy improves host's microecological balance, performance beneficial effect.In human body, animal body, useful bacterium or fungi mainly contains: clostridium butyricum, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus, actinomyces, saccharomycete etc.The most powerful product of function of research is mainly the composite reactive probio of above all kinds of the composition of the microorganisms in the world at present, and it is widely used in bioengineering, industrial or agricultural, food security and life and health field.Yet probio can be subject to the impact of hydrochloric acid in gastric juice and cholate conventionally in actual applications, only have a small amount of can arrival in enteron aisle, and the propagation that makes to reach the probio in enteron aisle due to factors such as nutritional deficiency, bile salt erosions is suppressed, it is former powerful to be difficult to performance.There are some researches show, edible fungi polysaccharide composition has growth promoting function to probio, and polysaccharide and probio arrive enteron aisle simultaneously, with probio synergy, can reach and increase the trophism of probiotics, the object such as functional.
Edible fungi polysaccharide is an isolated class active polysaccharide from fruit body of edible fungi, mycelium, zymotic fluid, and it not only can promote the growth of probio, strengthening immunity, the aspect such as antitumor has caused people's common concern in recent years.Edible fungi polysaccharide is mainly chitin and water-insoluble β-(1-3)-D-glucan, the feature with biological respinse modifier, as immunopotentiator and immunoactivator, can make it produce a large amount of immune globulin antibodies by promoting the propagation of B cell, thereby strengthen resistance against diseases; Wherein β-(1-3)-D-glucan has preventive and therapeutic effect to even genetic tumour allos, homology.In addition, it also has the antiviral and poly-effect of anti-freezing, can improve liver function and conciliate virulence, improves the utilization rate of animal hypoxia-bearing capability and oxygen, reduces the viscosity of blood.
In recent years, along with polyphenols at cleaning machine interior free yl, anti-lipid oxidation, delay the discovery of the aspect bioactive functions such as body aging, angiocardiopathy preventing, anti-cancer, radioresistance, its research is subject to extensive concern.Vine polyphenol is a kind of plant polyphenol kind active material, is extensively present in grape pip, Grape Skin and fruit juice.This class polyphenol is comprised of materials such as the phenolic acid classes such as epicatechin, flavanol compound, anthocyanin class, flavonols and polycondensation tannin, wherein abundant with the content of former anthocyanin, can reach 80%~85%.That vine polyphenol has is anti-oxidant, anti arteriosclerosis, resistance is different and the physiologically active such as anticancer and antiallergy.Vine polyphenol anti-stress effect better, also can be used for aquatic livestock and extend the time-to-live after its offshore, reduce feed coefficient.The synergy of vine polyphenol and edible fungi polysaccharide and probio not only can improve the effectiveness of active probiotic, plays the immunity and the prevention effect to various diseases that strengthen human body simultaneously.
Sustained release agent refers to after medication that sustained release medicine is to reach the preparation that extends drug effect object in a long time.Sodium alginate is the natural biological polysaccharide extracting from live in the brown alga of frigid zone or temperate zone seashore or bacterium.Alginate can be with polyvalent cation (as calcium ion and barium ions) in conjunction with forming solubility gel, and alginate has good biocompatibility, and these features make it can be used as the cyst material of release or embedding medicinal, albumen and cell.Because probio self is more fragile and be easily subject to the impact of surrounding environment factor, when it enters after body, easily be subject to the effect of hydrochloric acid in gastric juice and cholate, cause the quantity of probio to decline soon, cause the probio quantity finally entering in enteron aisle seldom can not effectively bring into play its prebiotic effect.Probio, vine polyphenol, edible fungi polysaccharide are prepared into sustained release agent after by alginate embedding; not only can play a physical barriers is used for protecting active ingredient (especially probio) wherein to avoid the impact of extraneous poor environment; and can guarantee that thereby its vigor arrives certain action site and brings into play its function, play the effect of slowly-releasing.
summary of the invention
The object of the invention is to by grape seed polyphenols, edible fungi polysaccharide and probio are acted synergistically, and be prepared into sustained release agent with alginate embedding, not only can delay senility, strengthen the resistance of human body, and can play the growth that suppresses pathogen, improving the effect of function of intestinal canal, is a kind of all-ages preparation with health care.
The preparation method who the present invention relates to a kind of probio sustained release agent containing plant extracts, is characterized in that, preparation method comprises the following steps:
(1) the edible fungi polysaccharide powder of the vine polyphenol powder of 1-50%, 1-50% is dissolved in the compound probiotic mixed liquor of 1-80%, obtains stoste A;
(2) stoste A is mixed in the ratio of 1:4-4:1 with the alginate of 0.1-50%, add the abundant swelling of glacial acetic acid of 0.1-20%, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise in the calcium chloride solution of 1-20% with syringe, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, wherein vine polyphenol purity >=50%.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, wherein edible fungi polysaccharide content >=60%.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, described compound probiotic be lactic acid bacteria class ( lactobacillus), Bifidobacterium class ( bifidobacteria) wherein one or more, its total viable count should>=10 6cfu/g.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, the gel slow-released agent obtaining is microspheric, and particle diameter is at 0.1-30mm.
The gel slow-released agent of preparing according to above-mentioned steps also can add in normal diet and use as feed addictive according to the ratio of 0.1-0.5%.
When the gel slow-released agent of preparing according to above-mentioned steps is used as feed addictive, compound probiotic used can be also photosynthetic bacteria class ( photosynthetic Bacteria), bacillus category ( bacillaceae) in one or more.
The correlation technique not describing in detail in the present invention is with reference to the existing technique in affiliated field.
Beneficial effect of the present invention be by preparation take sustained release agent that vine polyphenol, edible fungi polysaccharide and probio be primary raw material can contribute to delay senility, strengthen body immunity, anti-oxidant, antiviral, antitumor, regulate blood pressure blood fat, promote enteron aisle digestive system health etc., take alginate after wall material carries out embedding, can resist well the destructions of unfavorable conditions to content such as temperature, humidity; Alginate is a kind of semi permeability microporous film with some strength simultaneously, and its pellicle characteristic makes content optionally see through wall material, forms a kind of controlled release durg delivery system, makes its product have good functional character and bin stability.
the specific embodiment
?mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments, and the experimental technique of unreceipted actual conditions in the following example, completes according to the conventional method in affiliated field and condition.
Embodiment mono-:
(1) 20% vine polyphenol powder, 20% edible fungi polysaccharide powder are dissolved in 60% lactic acid bacteria and Bifidobacterium mixed liquor, are fully mixed to get stoste A;
(2) stoste A is mixed in the ratio of 1:1 with 4% alginate, add 2% the abundant swelling of glacial acetic acid, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise to syringe in 2% calcium chloride solution, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
Embodiment bis-:
(1) 10% vine polyphenol powder, 40% edible fungi polysaccharide powder are dissolved in to 50% lactic acid bacteria and Bifidobacterium mixed liquor, are fully mixed to get stoste A;
(2) stoste A is mixed in the ratio of 2:1 with 4% alginate, add 2% the abundant swelling of glacial acetic acid, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise to syringe in 4% calcium chloride solution, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.

Claims (7)

1. contain a preparation method for the probio sustained release agent of plant extracts, it is characterized in that, preparation method comprises the following steps:
(1) the edible fungi polysaccharide powder of the vine polyphenol powder of 1-50%, 1-50% is dissolved in the compound probiotic mixed liquor of 1-80%, obtains stoste A;
(2) stoste A is mixed in the ratio of 1:4-4:1 with the alginate of 0.1-50%, add the abundant swelling of glacial acetic acid of 0.1-20%, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise in the calcium chloride solution of 1-20% with syringe, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
2. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, wherein vine polyphenol purity >=50%.
3. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, wherein edible fungi polysaccharide content >=60%.
4. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, described compound probiotic be lactic acid bacteria class ( lactobacillus), Bifidobacterium class ( bifidobacteria) wherein one or more, its total viable count should>=10 6cfu/g.
5. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, the gel slow-released agent obtaining is microspheric, and particle diameter is at 0.1-30mm.
6. the gel slow-released agent of preparing according to method described in claim 1 also can add in normal diet and use as feed addictive according to the ratio of 0.1-0.5%.
7. when the gel slow-released agent of preparing according to method described in claim 1 is used as feed addictive, compound probiotic used can be photosynthetic bacteria class ( photosynthetic Bacteria), bacillus category ( bacillaceae) in one or more.
CN201410344986.4A 2014-07-21 2014-07-21 Preparation method for probiotic sustained release agent containing plant extracts Pending CN104095186A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105747060A (en) * 2016-02-26 2016-07-13 暨南大学 Health beverage capable of promoting multiplication of intestinal bifidobacterium as well as preparation method and application of health beverage
CN108703374A (en) * 2018-05-29 2018-10-26 广州梵荣生物科技有限公司 A kind of Genistein antioxidant composition microballoon and its preparation process
CN112890204A (en) * 2021-03-15 2021-06-04 中农宠物营养研究院(江苏)有限公司 Microcapsule using grape seed extract as prebiotics and preparation method thereof
CN113694007A (en) * 2021-09-02 2021-11-26 广东丸美生物技术股份有限公司 Probiotics containing active ingredients and preparation method and application thereof

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105747060A (en) * 2016-02-26 2016-07-13 暨南大学 Health beverage capable of promoting multiplication of intestinal bifidobacterium as well as preparation method and application of health beverage
CN108703374A (en) * 2018-05-29 2018-10-26 广州梵荣生物科技有限公司 A kind of Genistein antioxidant composition microballoon and its preparation process
CN112890204A (en) * 2021-03-15 2021-06-04 中农宠物营养研究院(江苏)有限公司 Microcapsule using grape seed extract as prebiotics and preparation method thereof
CN113694007A (en) * 2021-09-02 2021-11-26 广东丸美生物技术股份有限公司 Probiotics containing active ingredients and preparation method and application thereof
CN113694007B (en) * 2021-09-02 2022-12-23 广东丸美生物技术股份有限公司 Probiotics containing active ingredients and preparation method and application thereof

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