CN104095186A - Preparation method for probiotic sustained release agent containing plant extracts - Google Patents
Preparation method for probiotic sustained release agent containing plant extracts Download PDFInfo
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- CN104095186A CN104095186A CN201410344986.4A CN201410344986A CN104095186A CN 104095186 A CN104095186 A CN 104095186A CN 201410344986 A CN201410344986 A CN 201410344986A CN 104095186 A CN104095186 A CN 104095186A
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- 238000013268 sustained release Methods 0.000 title claims abstract description 34
- 239000012730 sustained-release form Substances 0.000 title claims abstract description 34
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 239000006041 probiotic Substances 0.000 title claims abstract description 15
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 15
- 239000000419 plant extract Substances 0.000 title claims abstract description 14
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 10
- 150000004676 glycans Chemical class 0.000 claims abstract description 23
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 23
- 235000013824 polyphenols Nutrition 0.000 claims abstract description 23
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 23
- 239000005017 polysaccharide Substances 0.000 claims abstract description 23
- 241000233866 Fungi Species 0.000 claims abstract description 22
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims abstract description 11
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229940072056 alginate Drugs 0.000 claims abstract description 11
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 11
- 229920000615 alginic acid Polymers 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 10
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 5
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- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
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- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical class C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 241000186046 Actinomyces Species 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
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- CITFYDYEWQIEPX-UHFFFAOYSA-N Flavanol Natural products O1C2=CC(OCC=C(C)C)=CC(O)=C2C(=O)C(O)C1C1=CC=C(O)C=C1 CITFYDYEWQIEPX-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
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- 230000002180 anti-stress Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 229910001422 barium ion Inorganic materials 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
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- 210000004027 cell Anatomy 0.000 description 1
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- 239000002131 composite material Substances 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
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- 208000031513 cyst Diseases 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
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- 210000002249 digestive system Anatomy 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- -1 flavanol compound Chemical class 0.000 description 1
- 235000011987 flavanols Nutrition 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000002216 flavonol derivatives Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 229940087559 grape seed Drugs 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000000091 immunopotentiator Effects 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000003607 modifier Substances 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 238000006068 polycondensation reaction Methods 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention discloses a preparation method for probiotic sustained release agent containing plant extracts. The preparation method comprises: (1) dissolving grape polyphenol powder and edible fungi polysaccharide powder in a mixed liquor of complex probiotics to get stock solution A; (2) homogeneously mixing the stock solution A with alginate at a certain ratio and adding acetic acid to conducting fully swelling so as to get a stock solution B; (3) the stock solution B was added dropwisely to a solution of calcium chloride with a syringe to obtain a sustained release gel microspheres; (4) collecting the sustained release gel microspheres and drying at room temperature to get the probiotic sustained release agent containing grape polyphenols and edible fungi polysaccharides. With the synergic effect of grape polyphenols, edible fungi polysaccharides and probiotics, the present invention not only can improve the effectiveness of the active probiotics, but also strengthen the body's immune system and prevent various diseases.
Description
Technical field
The present invention relates to processing of farm products field, be specifically related to a kind of preparation method of the probio sustained release agent containing plant extracts.
Background technology
Probio (Probiotics) is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system, thereby can produce the active beneficial microorganism general name that definite health efficacy improves host's microecological balance, performance beneficial effect.In human body, animal body, useful bacterium or fungi mainly contains: clostridium butyricum, lactic acid bacteria, Bifidobacterium, lactobacillus acidophilus, actinomyces, saccharomycete etc.The most powerful product of function of research is mainly the composite reactive probio of above all kinds of the composition of the microorganisms in the world at present, and it is widely used in bioengineering, industrial or agricultural, food security and life and health field.Yet probio can be subject to the impact of hydrochloric acid in gastric juice and cholate conventionally in actual applications, only have a small amount of can arrival in enteron aisle, and the propagation that makes to reach the probio in enteron aisle due to factors such as nutritional deficiency, bile salt erosions is suppressed, it is former powerful to be difficult to performance.There are some researches show, edible fungi polysaccharide composition has growth promoting function to probio, and polysaccharide and probio arrive enteron aisle simultaneously, with probio synergy, can reach and increase the trophism of probiotics, the object such as functional.
Edible fungi polysaccharide is an isolated class active polysaccharide from fruit body of edible fungi, mycelium, zymotic fluid, and it not only can promote the growth of probio, strengthening immunity, the aspect such as antitumor has caused people's common concern in recent years.Edible fungi polysaccharide is mainly chitin and water-insoluble β-(1-3)-D-glucan, the feature with biological respinse modifier, as immunopotentiator and immunoactivator, can make it produce a large amount of immune globulin antibodies by promoting the propagation of B cell, thereby strengthen resistance against diseases; Wherein β-(1-3)-D-glucan has preventive and therapeutic effect to even genetic tumour allos, homology.In addition, it also has the antiviral and poly-effect of anti-freezing, can improve liver function and conciliate virulence, improves the utilization rate of animal hypoxia-bearing capability and oxygen, reduces the viscosity of blood.
In recent years, along with polyphenols at cleaning machine interior free yl, anti-lipid oxidation, delay the discovery of the aspect bioactive functions such as body aging, angiocardiopathy preventing, anti-cancer, radioresistance, its research is subject to extensive concern.Vine polyphenol is a kind of plant polyphenol kind active material, is extensively present in grape pip, Grape Skin and fruit juice.This class polyphenol is comprised of materials such as the phenolic acid classes such as epicatechin, flavanol compound, anthocyanin class, flavonols and polycondensation tannin, wherein abundant with the content of former anthocyanin, can reach 80%～85%.That vine polyphenol has is anti-oxidant, anti arteriosclerosis, resistance is different and the physiologically active such as anticancer and antiallergy.Vine polyphenol anti-stress effect better, also can be used for aquatic livestock and extend the time-to-live after its offshore, reduce feed coefficient.The synergy of vine polyphenol and edible fungi polysaccharide and probio not only can improve the effectiveness of active probiotic, plays the immunity and the prevention effect to various diseases that strengthen human body simultaneously.
Sustained release agent refers to after medication that sustained release medicine is to reach the preparation that extends drug effect object in a long time.Sodium alginate is the natural biological polysaccharide extracting from live in the brown alga of frigid zone or temperate zone seashore or bacterium.Alginate can be with polyvalent cation (as calcium ion and barium ions) in conjunction with forming solubility gel, and alginate has good biocompatibility, and these features make it can be used as the cyst material of release or embedding medicinal, albumen and cell.Because probio self is more fragile and be easily subject to the impact of surrounding environment factor, when it enters after body, easily be subject to the effect of hydrochloric acid in gastric juice and cholate, cause the quantity of probio to decline soon, cause the probio quantity finally entering in enteron aisle seldom can not effectively bring into play its prebiotic effect.Probio, vine polyphenol, edible fungi polysaccharide are prepared into sustained release agent after by alginate embedding; not only can play a physical barriers is used for protecting active ingredient (especially probio) wherein to avoid the impact of extraneous poor environment; and can guarantee that thereby its vigor arrives certain action site and brings into play its function, play the effect of slowly-releasing.
summary of the invention
The object of the invention is to by grape seed polyphenols, edible fungi polysaccharide and probio are acted synergistically, and be prepared into sustained release agent with alginate embedding, not only can delay senility, strengthen the resistance of human body, and can play the growth that suppresses pathogen, improving the effect of function of intestinal canal, is a kind of all-ages preparation with health care.
The preparation method who the present invention relates to a kind of probio sustained release agent containing plant extracts, is characterized in that, preparation method comprises the following steps:
(1) the edible fungi polysaccharide powder of the vine polyphenol powder of 1-50%, 1-50% is dissolved in the compound probiotic mixed liquor of 1-80%, obtains stoste A;
(2) stoste A is mixed in the ratio of 1:4-4:1 with the alginate of 0.1-50%, add the abundant swelling of glacial acetic acid of 0.1-20%, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise in the calcium chloride solution of 1-20% with syringe, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, wherein vine polyphenol purity >=50%.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, wherein edible fungi polysaccharide content >=60%.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, described compound probiotic be lactic acid bacteria class (
lactobacillus), Bifidobacterium class (
bifidobacteria) wherein one or more, its total viable count should>=10
6cfu/g.
The preparation method of a kind of probio sustained release agent containing plant extracts of the present invention, is characterized in that, the gel slow-released agent obtaining is microspheric, and particle diameter is at 0.1-30mm.
The gel slow-released agent of preparing according to above-mentioned steps also can add in normal diet and use as feed addictive according to the ratio of 0.1-0.5%.
When the gel slow-released agent of preparing according to above-mentioned steps is used as feed addictive, compound probiotic used can be also photosynthetic bacteria class (
photosynthetic Bacteria), bacillus category (
bacillaceae) in one or more.
The correlation technique not describing in detail in the present invention is with reference to the existing technique in affiliated field.
Beneficial effect of the present invention be by preparation take sustained release agent that vine polyphenol, edible fungi polysaccharide and probio be primary raw material can contribute to delay senility, strengthen body immunity, anti-oxidant, antiviral, antitumor, regulate blood pressure blood fat, promote enteron aisle digestive system health etc., take alginate after wall material carries out embedding, can resist well the destructions of unfavorable conditions to content such as temperature, humidity; Alginate is a kind of semi permeability microporous film with some strength simultaneously, and its pellicle characteristic makes content optionally see through wall material, forms a kind of controlled release durg delivery system, makes its product have good functional character and bin stability.
the specific embodiment
?mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments, and the experimental technique of unreceipted actual conditions in the following example, completes according to the conventional method in affiliated field and condition.
Embodiment mono-:
(1) 20% vine polyphenol powder, 20% edible fungi polysaccharide powder are dissolved in 60% lactic acid bacteria and Bifidobacterium mixed liquor, are fully mixed to get stoste A;
(2) stoste A is mixed in the ratio of 1:1 with 4% alginate, add 2% the abundant swelling of glacial acetic acid, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise to syringe in 2% calcium chloride solution, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
Embodiment bis-:
(1) 10% vine polyphenol powder, 40% edible fungi polysaccharide powder are dissolved in to 50% lactic acid bacteria and Bifidobacterium mixed liquor, are fully mixed to get stoste A;
(2) stoste A is mixed in the ratio of 2:1 with 4% alginate, add 2% the abundant swelling of glacial acetic acid, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise to syringe in 4% calcium chloride solution, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
Claims (7)
1. contain a preparation method for the probio sustained release agent of plant extracts, it is characterized in that, preparation method comprises the following steps:
(1) the edible fungi polysaccharide powder of the vine polyphenol powder of 1-50%, 1-50% is dissolved in the compound probiotic mixed liquor of 1-80%, obtains stoste A;
(2) stoste A is mixed in the ratio of 1:4-4:1 with the alginate of 0.1-50%, add the abundant swelling of glacial acetic acid of 0.1-20%, obtain stoste B;
(3) above-mentioned stoste B is added drop-wise in the calcium chloride solution of 1-20% with syringe, obtains gel rubber sustained-release microballoon;
(4) collect above-mentioned gel rubber sustained-release microballoon, drying at room temperature, obtains the probio sustained release agent that contains vine polyphenol, edible fungi polysaccharide.
2. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, wherein vine polyphenol purity >=50%.
3. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, wherein edible fungi polysaccharide content >=60%.
4. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, described compound probiotic be lactic acid bacteria class (
lactobacillus), Bifidobacterium class (
bifidobacteria) wherein one or more, its total viable count should>=10
6cfu/g.
5. the preparation method of a kind of probio sustained release agent containing plant extracts according to claim 1, is characterized in that, the gel slow-released agent obtaining is microspheric, and particle diameter is at 0.1-30mm.
6. the gel slow-released agent of preparing according to method described in claim 1 also can add in normal diet and use as feed addictive according to the ratio of 0.1-0.5%.
7. when the gel slow-released agent of preparing according to method described in claim 1 is used as feed addictive, compound probiotic used can be photosynthetic bacteria class (
photosynthetic Bacteria), bacillus category (
bacillaceae) in one or more.
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| CN201410344986.4A CN104095186A (en) | 2014-07-21 | 2014-07-21 | Preparation method for probiotic sustained release agent containing plant extracts |
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| CN201410344986.4A CN104095186A (en) | 2014-07-21 | 2014-07-21 | Preparation method for probiotic sustained release agent containing plant extracts |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105747060A (en) * | 2016-02-26 | 2016-07-13 | 暨南大学 | Health beverage capable of promoting multiplication of intestinal bifidobacterium as well as preparation method and application of health beverage |
| CN108703374A (en) * | 2018-05-29 | 2018-10-26 | 广州梵荣生物科技有限公司 | A kind of Genistein antioxidant composition microballoon and its preparation process |
| CN112890204A (en) * | 2021-03-15 | 2021-06-04 | 中农宠物营养研究院(江苏)有限公司 | Microcapsule using grape seed extract as prebiotics and preparation method thereof |
| CN113694007A (en) * | 2021-09-02 | 2021-11-26 | 广东丸美生物技术股份有限公司 | Probiotics containing active ingredients and preparation method and application thereof |
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