CN104094852A - Method for promoting rooting of strong seedlings of cassava tissue culture seedlings - Google Patents
Method for promoting rooting of strong seedlings of cassava tissue culture seedlings Download PDFInfo
- Publication number
- CN104094852A CN104094852A CN201410354275.5A CN201410354275A CN104094852A CN 104094852 A CN104094852 A CN 104094852A CN 201410354275 A CN201410354275 A CN 201410354275A CN 104094852 A CN104094852 A CN 104094852A
- Authority
- CN
- China
- Prior art keywords
- seedling
- rooting
- cassava
- seedlings
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000001737 promoting effect Effects 0.000 title abstract 2
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 title 1
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 68
- 230000004083 survival effect Effects 0.000 claims abstract description 34
- 239000001963 growth medium Substances 0.000 claims abstract description 26
- 229930006000 Sucrose Natural products 0.000 claims abstract description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 16
- 239000002609 medium Substances 0.000 claims abstract description 16
- 238000005286 illumination Methods 0.000 claims abstract description 14
- 238000012549 training Methods 0.000 claims description 90
- 238000005728 strengthening Methods 0.000 claims description 41
- 239000005720 sucrose Substances 0.000 claims description 16
- 230000035755 proliferation Effects 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 9
- 230000003020 moisturizing effect Effects 0.000 claims description 8
- 238000009423 ventilation Methods 0.000 claims description 8
- 239000011159 matrix material Substances 0.000 claims description 7
- 239000003415 peat Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- 239000002689 soil Substances 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 230000006698 induction Effects 0.000 abstract description 3
- 229960004793 sucrose Drugs 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 description 12
- 238000010899 nucleation Methods 0.000 description 11
- 230000018044 dehydration Effects 0.000 description 8
- 238000006297 dehydration reaction Methods 0.000 description 8
- 239000012882 rooting medium Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 description 2
- 241000221017 Euphorbiaceae Species 0.000 description 2
- 244000017020 Ipomoea batatas Species 0.000 description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 description 2
- 239000005985 Paclobutrazol Substances 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000012090 tissue culture technique Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 101000654381 Homo sapiens Sodium channel protein type 8 subunit alpha Proteins 0.000 description 1
- 235000004456 Manihot esculenta Nutrition 0.000 description 1
- 102100031371 Sodium channel protein type 8 subunit alpha Human genes 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- DFWFIQKMSFGDCQ-UHFFFAOYSA-N deethylatrazine Chemical compound CC(C)NC1=NC(N)=NC(Cl)=N1 DFWFIQKMSFGDCQ-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000012883 rooting culture medium Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for promoting rooting of strong seedlings of cassava tissue culture seedlings, relates to the field of agricultural propagation methods. The method comprises the steps of successive transfer culture of tissue culture seedlings, rooting culture of strong seedlings of the tissue culture seedlings and transplant and survival of hardening-seedlings of the rooting tissue culture seedlings, wherein the successive transfer culture of the tissue culture seedlings comprises the steps as follows: germfree tissue culture seedlings are sliced into single bud stems, and then the stems are transferred to a culture medium A for multiplication successive transfer culture; the rooting culture of the strong seedlings of the tissue culture seedlings comprises the steps as follows: multiplication successive transfer cultured cassava tissue culture seedling single bud stems are then transferred to a culture medium B of a culture room for culture at the culture temperature of 26 to 28 DEG C, with the illumination intensity of 1500 to 2000 lx, within the illumination time of 12 h/d and the culture period of 40 d, wherein the culture medium B is a semisolid medium of cane sugar with MS (or 1/2 MS) + 0.01-0.02 mg/LNAA + 40-50 g/L and with the PH value of 5.8. The method overcomes the problems that the cassava tissue culture seedlings are fragile and easy to broke, easy to wither due to water loss, and meanwhile, the induction quality of seedlings and roots is good, the surviving rate of transplant of the cassava tissue culture seedlings is improved, the working efficiency is improved, and the production cost is reduced.
Description
Technical field
The present invention relates to agriculture propagation method field, be specially the propagation method field of cassava, relate in particular to a kind of method of cassava group training seedling strengthening seedling and rooting.
Background technology
Cassava (Manihot esculenta Crantz) has another name called cassava, tree sweet potato, wood sweet potato, it is Euphorbiaceae (Euphorbiaceae) cassava (Manihot) plant, being one of three large tuber crops, is global the sixth-largest cereal crops, the third-largest cereal crops in hot-zone, edible, feeding and industrial, has huge market development space in China.
Because complicated, the sexual filial generation of cassava genetic background exists serious separation, in production, conventionally adopt propagation of seed stock, this modes of reproduction has limited popularization and the production of cassava improved Varieties to a certain extent.Tissue culture technique can obtain a large amount of brood bodies at short notice, and this promotes significant to accelerating stock breeding and new varieties.Meanwhile, tissue culture technique is also the important foundation of carrying out cassava biotechnology breeding.
But here more crisp easily broken, the easy dehydration of the cassava of cultivating group training seedling withers, and causes transplanting survival rate lower.At present domestic also have the result of the test of on a small quantity cassava tissue being cultivated to report.As, Li Ping etc. (2012), research shows, adds 4mg/L paclobutrazol and can improve cassava group training transplantation of seedlings survival rate, and the research of Dan Ben seminar shows, paclobutrazol can not effectively promote cassava group training seedling strong sprout, and cassava group training transplantation of seedlings survival rate is also lower.A lot of research units adopt the general cassava group training seedling rooting culture medium prescription in International Tropical agricultural research center (CIAT) to organize training seedling rooting and cultivate, it is the semisolid culturemedium (pH=5.8) that 1/3MS adds 0.02mg/LNAA and 20g/L sucrose, the research of Dan Ben seminar shows, under this culture medium condition, here more crisp easily broken, the easy dehydration of cassava group training seedling of cultivating withers, cause transplanting survival rate lower, often lower than 50%.
Group training seedling rooting quality has directly determined the survival rate that the later stage transplants, and the quality of taking root is directly subject to again break up the impact of seedling quality early stage, if seedling is short and small, thin and delicate, quality of rooting is also poor.The difficult problem that cassava group training seedling therefore urgently to be resolved hurrily occurs in producing.
As can be seen here, current cassava group training seedling rooting cultural method is failed to meet cassava group training seedling and is produced and scientific research demand, therefore, be necessary cassava group training seedling strengthening seedling and rooting medium to further investigate completely, improve cassava group training transplantation of seedlings survival rate, and then increase work efficiency, reduce production costs.
Summary of the invention
The object of the invention is to: for the problem of above-mentioned existence, a kind of method of cassava group training seedling strengthening seedling and rooting is provided, overcome traditional cassava group training seedling more crisp easily broken, easy dehydration in acclimatization and transplants process problem such as here wither, improve cassava group training transplantation of seedlings survival rate, and increase work efficiency, reduce production costs.
The technical solution used in the present invention is:
A kind of method of cassava group training seedling strengthening seedling and rooting, its step comprises the cultivation of group training seedling subculture, group training seedling strengthening seedling and rooting is cultivated, the training tissue culture seedling transplant survival of taking root, it is that aseptic cassava group training seedling is cut into stem-segment with single bud that described group of training seedling subculture cultivated, and be transferred to and in culture medium A, carry out proliferation and subculture cultivation, described group of training seedling strengthening seedling and rooting cultivated and is: the cassava group training seedling stem-segment with single bud after proliferation and subculture is cultivated, be transferred in medium B and cultivate, cultivation temperature is 27 ± 1 ℃, intensity of illumination is 1500～2000lx, light application time is 12h/d, cultivation cycle is 40d, wherein said medium B is the semisolid culturemedium of MS (or 1/2MS)+0.01～0.02mg/LNAA+40～50g/L sucrose, pH value is 5.8.
The described training tissue culture seedling transplant survival of taking root is: will grow to the aseptic strengthening seedling and rooting Zu Peimiaocong immigration greenhouse hardening 7～10d of culturing room of 5～7cm, then take out the group training seedling after hardening and clean, transplant to cultivation matrix, transplant in 7～10d and want temperature control ventilation moisturizing, after transplanting 30d, add up transplanting survival rate.
As preferred version of the present invention, described culture medium A is the semisolid culturemedium of MS+0.01～0.02mg/LBA+0.02mg/LNAA+30g/L sucrose, and pH value is 5.8.
Further, it is to be 27 ± 1 ℃ in cultivation temperature that described proliferation and subculture is cultivated, and intensity of illumination is 1500～2000lx, under the condition that light application time is 12h/d, cultivates, and its cultivation cycle is 40d.
Further, described cultivation matrix is comprised of peat soil, fine sand and perlite and by volume for the ratio of 2:1:1 mixes.
In sum, owing to having adopted technique scheme, the invention has the beneficial effects as follows: adopt cassava group training seedling strengthening seedling and rooting method of the present invention, improved the cassava group training seedling quality of taking root, cassava tissue culture seeding stem segment obviously increases thick, group training seedling dry matter content obviously increases, the induction quality of Miao Yugen is good, overcome traditional cassava group training seedling more crisp easily broken, easy dehydration in acclimatization and transplants process problem such as here wither, obviously improved cassava group training transplantation of seedlings survival rate, transplanting survival rate can be up to more than 95%, and then improved operating efficiency, reduced production cost.
Embodiment
Below in conjunction with embodiment, foregoing invention content of the present invention is described in further detail, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following embodiment.
Embodiment 1
First, aseptic cassava group training seedling is cut into stem-segment with single bud, and be transferred to and in culture medium A, carry out proliferation and subculture cultivation, culture medium A is the semisolid culturemedium of MS+0.01mg/LBA+0.02mg/LNAA+30g/L sucrose, pH value is 5.8, in cultivation temperature, be that 27 ± 1 ℃, intensity of illumination are to cultivate under 1500lx, the light application time condition that is 12h/d, cultivation cycle is 40d.Then, cassava group training seedling stem-segment with single bud after proliferation and subculture is cultivated, be transferred in medium B and cultivate, medium B is the semisolid culturemedium of MS+0.01mg/LNAA+40g/L sucrose, pH value is 5.8, and cultivation temperature is 27 ± 1 ℃, and intensity of illumination is 1500lx, light application time is 12h/d, and cultivation cycle is 40d.Finally, the aseptic strengthening seedling and rooting Zu Peimiaocong immigration green house hardening 7d of culturing room of 5cm will be grown to, then take out the group training seedling after hardening and clean, transplant to being formed by peat soil, fine sand and perlite also by volume in the cultivation matrix mixing for the ratio of 2:1:1, transplant in 7d and want temperature control ventilation moisturizing, after transplanting 30d, add up transplanting survival rate.
After subculture is cultivated, adopt the method for embodiment 1 to carry out strengthening seedling and rooting to 100 strain group training seedlings, rooting rate is 100%, and move to after medium, 99% cassava tissue culture seeding stem segment obviously increases thick, surpasses more than 2mm, average about 2.5mm, group training seedling dry matter content increases, and 99% group training seedling all bears 3 above roots, the long 2cm that surpasses of root, average about 3.5cm, and adventive root is sturdy, reach transplanting requirement, cassava group training transplantation of seedlings survival rate can be up to 95.5%.
And common group of training seedling strengthening seedling and rooting method, although rooting rate can reach more than 95%, the tissue culture seeding stem segment of taking root is very thin less than 2mm, easily crisp easily broken, easily dehydration is wilted, and adventive root a little less than, capillary root is undeveloped, causes transplanting survival rate often lower than 50%.
Embodiment 2
First, aseptic cassava group training seedling is cut into stem-segment with single bud, and be transferred to and in culture medium A, carry out proliferation and subculture cultivation, culture medium A is the semisolid culturemedium of MS+0.01mg/LBA+0.02mg/LNAA+30g/L sucrose, pH value is 5.8, in cultivation temperature, be that 27 ± 1 ℃, intensity of illumination are to cultivate under 1500lx, the light application time condition that is 12h/d, cultivation cycle is 40d.Then, cassava group training seedling stem-segment with single bud after proliferation and subculture is cultivated, be transferred in medium B and cultivate, medium B is the semisolid culturemedium of MS+0.01mg/LNAA+45g/L sucrose, pH value is 5.8, and cultivation temperature is 27 ± 1 ℃, and intensity of illumination is 1500lx, light application time is 12h/d, and cultivation cycle is 40d.Finally, the aseptic strengthening seedling and rooting Zu Peimiaocong immigration green house hardening 7d of culturing room of 5cm will be grown to, then take out the group training seedling after hardening and clean, transplant to being formed by peat soil, fine sand and perlite in the cultivation matrix that also ratio of 2:1:1 mixes by volume, transplant in 7d and want temperature control ventilation moisturizing, after transplanting 30d, add up transplanting survival rate.
After subculture is cultivated, adopt the method for embodiment 2 to carry out strengthening seedling and rooting to 110 strain group training seedlings, rooting rate is 100%, and move to after medium, 99% cassava tissue culture seeding stem segment obviously increases thick, average about 2.6mm, group training seedling dry matter content increases, and 98% group training seedling all bears 3 above roots, and root is long all over 2cm, average out to 3.5cm, and adventive root is sturdy, capillary root is flourishing, reaches transplanting requirement, and cassava group training transplantation of seedlings survival rate is up to 96.8%.
And common group of training seedling strengthening seedling and rooting method, although rooting rate can reach more than 95%, but only have 46% group training seedling to bear 3 above roots, root is long generally between 1～1.5 centimetre, tissue culture seeding stem segment is very thin less than 2mm, easily crisp easily broken, and easily dehydration is wilted, and adventive root a little less than, capillary root is undeveloped, causes transplanting survival rate often lower than 50%.
Embodiment 3
First, aseptic cassava group training seedling is cut into stem-segment with single bud, and be transferred to and in culture medium A, carry out proliferation and subculture cultivation, culture medium A is the semisolid culturemedium of MS+0.01mg/LBA+0.02mg/LNAA+30g/L sucrose, pH value is 5.8, in cultivation temperature, be that 27 ± 1 ℃, intensity of illumination are to cultivate under 1500lx, the light application time condition that is 12h/d, cultivation cycle is 40d.Then, the stem-segment with single bud of the cassava group training seedling after proliferation and subculture is cultivated, be transferred in the medium B of culturing room and cultivate, medium B is the semisolid culturemedium of MS+0.01mg/LNAA+50g/L sucrose, pH value is 5.8, and cultivation temperature is 27 ± 1 ℃, and intensity of illumination is 1500lx, light application time is 12h/d, and cultivation cycle is 40d.Finally, the aseptic strengthening seedling and rooting Zu Peimiaocong immigration greenhouse hardening 7d of culturing room of 5cm will be grown to, then take out the group training seedling after hardening and clean, transplant to being formed by peat soil, fine sand and perlite also by volume in the cultivation matrix mixing for the ratio of 2:1:1, transplant in 7d and want temperature control ventilation moisturizing, after transplanting 30d, add up transplanting survival rate.
After subculture is cultivated, adopt the method for embodiment 3 to carry out strengthening seedling and rooting to 120 strain group training seedlings, rooting rate is 100%, and move to after medium, 99% cassava tissue culture seeding stem segment obviously increases thick, average about 2.8mm, group training seedling dry matter content increases, and 99% group training seedling all bears 3 above roots, and root is long all over 2cm, average out to 3.5cm, and adventive root is sturdy, capillary root is flourishing, reaches transplanting requirement, and cassava group training transplantation of seedlings survival rate can be up to 96%.
And common group of training seedling strengthening seedling and rooting method, although rooting rate can reach more than 95%, but only have 48% group training seedling to bear 3 above roots, root length is generally between 1～1.5 centimetre, and tissue culture seeding stem segment is very thin less than 2mm, easily crisp easily broken, easily dehydration is wilted, and adventive root a little less than, capillary root is undeveloped, causes transplanting survival rate often lower than 50%.
Embodiment 4
Be with embodiment 1～3 difference: in strengthening seedling and rooting medium B, basal medium MS replaces with 1/2MS.
Adopt the method for embodiment 4 to carry out strengthening seedling and rooting to cassava group training seedling, the cassava tissue culture seeding stem segment obtaining obviously increases thick, group training seedling dry matter content increases, rooting rate is 100%, and move to after medium, 99% cassava tissue culture seeding stem segment obviously increases thick, surpass more than 2cm, average about 2.6mm, group training seedling dry matter content increases, and 99% group training seedling all bears 3 above roots, root is long all over 2cm, and adventive root is sturdy, and capillary root is flourishing, reach transplanting requirement, cassava group training transplantation of seedlings survival rate can be up to more than 95%.Basic identical with the effect reaching of embodiment 1～3.
Embodiment 5
Be with embodiment 1～4 difference: in strengthening seedling and rooting medium B, the concentration of growth hormone NAA is 0.02mg/L.
Embodiment 6
Be with embodiment 1～5 difference: in proliferation and subculture culture medium A, the concentration of basic element of cell division BA is 0.02mg/L.
Embodiment 7
Be with embodiment 1～6 difference: in the condition of culture that proliferation and subculture is cultivated and strengthening seedling and rooting is cultivated, intensity of illumination is 2000lx, and other conditions are constant.
Embodiment 8
Be with embodiment 1～7 difference: by grow to 6 or the aseptic strengthening seedling and rooting Zu Peimiaocong culturing room of 7cm move into greenhouse hardening 10d, transplant and in 10d, note cooling ventilation moisturizing.
Embodiment 9
Be with embodiment 1～8 difference: by grow to 6 or the aseptic strengthening seedling and rooting Zu Peimiaocong culturing room of 7cm move into greenhouse hardening 7d, transplant and in 7d, note cooling ventilation moisturizing.
Embodiment 10
Be with embodiment 1～9 difference: by grow to 6 or the aseptic strengthening seedling and rooting Zu Peimiaocong culturing room of 7cm move into greenhouse hardening 10d, transplant and in 10d, want temperature control ventilation moisturizing.
Adopt the strengthening seedling and rooting method of above embodiment 5～10, the cassava obtaining group training seedling, stem section obviously increases thick, and rooting rate reaches 100%, and transplanting survival rate can reach more than 95%.Basic identical with the effect that embodiment 1～4 obtains.
Now do following processing, and using embodiment 1～3 respectively as experimental group 1, experimental group 2, experimental group 3; Its cultivation temperature, intensity of illumination, cultivation cycle are identical, and variable is sucrose concentration in medium B, control group of the present invention is set and makes comparisons with experimental group, and the group training transplantation of seedlings of adding up after strengthening seedling and rooting adds up survival rate after 30 days, and statistical data is as table 1:
Control group 1: with embodiment 1 contrast, the component formula of its strengthening seedling and rooting medium B is MS+0.01mg/LNAA+30g/L sucrose, and other conditions are constant.
Control group 2: with embodiment 1 contrast, the component formula of its strengthening seedling and rooting medium B is MS+0.01mg/LNAA+20g/L sucrose, and other conditions are constant.
Control group 3: with embodiment 1 contrast, the component formula of its strengthening seedling and rooting medium B is MS+0.01mg/LNAA+55g/L sucrose, and other conditions are constant.
Impact and the transplanting survival rate thereof of table 1 different sucrose on group training seedling strengthening seedling and rooting
Interpretation:
Known by table 1, experimental group 1～3 and control group 1～3 contrast, wherein, although the cassava that control group 1～2 obtains group training seedling rooting rate can reach 100%, but group training seedling is very thin, in acclimatization and transplants process, easily crisp easily broken, easy dehydration is wilted, and transplanting survival rate is lower than 40%; Although the cassava that control group 3 obtains group training seedling stem section is thicker, rooting rate only has 78.2%, and group training seedling is short and small, causes transplanting survival rate to only have 72.4%.Experimental group 1～3, group training seedling, after subculture is cultivated, adopts the inventive method to carry out strengthening seedling and rooting to group training seedling, and rooting rate is 100%, and tissue culture seeding stem segment obviously increases thick, surpass 2.5mm, group training seedling dry matter content increases, and the long 3.0cm that surpasses of root, more than height of seedling reaches 4.0cm, and adventive root is sturdy, reach transplanting requirement, cassava group training transplantation of seedlings survival rate can be up to more than 95%.
The invention is not restricted to above-mentioned experimental group, embodiment 4～10 also can be used as experimental group, and the effect of the effect obtaining and embodiment 1～3 is basic identical, therefore at this, does not repeat.
In sum, adopt cassava group training seedling strengthening seedling and rooting method of the present invention, cassava tissue culture seeding stem segment obviously increases thick, group training seedling dry matter content obviously increases, improved cassava group training transplantation of seedlings survival rate, the induction quality of Miao Yugen is good, and overall transplanting survival rate can be up to more than 95%, and then improved operating efficiency, reduced production cost.
Claims (4)
1. a cassava is organized the method for training seedling strengthening seedling and rooting, its step comprises that group training seedling subculture is cultivated, group training seedling strengthening seedling and rooting is cultivated, the training tissue culture seedling transplant survival of taking root, it is that aseptic cassava group training seedling is cut into stem-segment with single bud that described group of training seedling subculture cultivated, and be transferred in culture medium A and carry out proliferation and subculture cultivation, it is characterized in that:
It is the cassava group training seedling stem-segment with single bud after proliferation and subculture is cultivated that described group of training seedling strengthening seedling and rooting cultivated, be transferred in medium B and cultivate, cultivation temperature is 27 ± 1 ℃, intensity of illumination is 1500～2000lx, light application time is 12h/d, cultivation cycle is 40d, and wherein said medium B is the semisolid culturemedium of MS (or 1/2MS)+0.01～0.02mg/LNAA+40～50g/L sucrose, and pH value is 5.8;
The described training tissue culture seedling transplant survival of taking root is to grow to the aseptic strengthening seedling and rooting Zu Peimiaocong immigration green house hardening 7～10d of culturing room of 5～7cm, then take out the group training seedling after hardening and clean, transplant to cultivation matrix, transplant ventilation moisturizing in 7～10d, after transplanting 30d, add up transplanting survival rate.
2. the method for a kind of cassava group training seedling strengthening seedling and rooting as claimed in claim 1, is characterized in that: described culture medium A is the semisolid culturemedium of MS+0.01～0.02mg/LBA+0.02mg/LNAA+30g/L sucrose, and pH value is 5.8.
3. a kind of cassava group as claimed in claim 1 is trained the method for seedling strengthening seedling and rooting, it is characterized in that: it is to be 27 ± 1 ℃ in cultivation temperature that described proliferation and subculture is cultivated, intensity of illumination is 1500～2000lx, under the condition that light application time is 12h/d, cultivates, and its cultivation cycle is 40d.
4. a kind of cassava group as claimed in claim 1 is trained the method for seedling strengthening seedling and rooting, the method that it is characterized in that a kind of cassava group training seedling strengthening seedling and rooting as claimed in claim 4, is characterized in that: described cultivation matrix is formed also by volume and to be mixed for the ratio of 2:1:1 by peat soil, fine sand and perlite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410354275.5A CN104094852B (en) | 2014-07-24 | 2014-07-24 | A kind of method of cassava plantlet in vitro strengthening seedling and rooting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410354275.5A CN104094852B (en) | 2014-07-24 | 2014-07-24 | A kind of method of cassava plantlet in vitro strengthening seedling and rooting |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104094852A true CN104094852A (en) | 2014-10-15 |
| CN104094852B CN104094852B (en) | 2016-04-06 |
Family
ID=51663689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410354275.5A Active CN104094852B (en) | 2014-07-24 | 2014-07-24 | A kind of method of cassava plantlet in vitro strengthening seedling and rooting |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104094852B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258961A (en) * | 2016-08-11 | 2017-01-04 | 广西作物遗传改良生物技术重点开放实验室 | A kind of method of liquid culture Maninot esculenta crantz. Potato microtuber |
| CN110558229A (en) * | 2019-09-30 | 2019-12-13 | 海南大学 | Seedling hardening method for remarkably improving transplanting survival rate of cassava tissue culture seedlings |
| CN113142056A (en) * | 2021-04-30 | 2021-07-23 | 中国农业科学院油料作物研究所 | Flax rooting culture medium and rooting promoting method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997044473A1 (en) * | 1996-05-20 | 1997-11-27 | Coöperatieve Verkoop- En Productievereniging Van Aardappelmeel En Derivaten Avebe B.A. | Methods for producing and transforming cassave protoplasts |
| CN101347097A (en) * | 2007-07-17 | 2009-01-21 | 朱文丽 | Method of somatic embryogenesis of cassava and rapid propagation of regenerated plant |
| CN102783415A (en) * | 2012-07-12 | 2012-11-21 | 中国热带农业科学院热带作物品种资源研究所 | Method for conservation in vitro of cassava germplasm resources with stability and high efficiency |
| CN103122340A (en) * | 2011-11-18 | 2013-05-29 | 中国科学院上海生命科学研究院 | Method for improving agronomic characters of cassava and application of method |
-
2014
- 2014-07-24 CN CN201410354275.5A patent/CN104094852B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997044473A1 (en) * | 1996-05-20 | 1997-11-27 | Coöperatieve Verkoop- En Productievereniging Van Aardappelmeel En Derivaten Avebe B.A. | Methods for producing and transforming cassave protoplasts |
| CN101347097A (en) * | 2007-07-17 | 2009-01-21 | 朱文丽 | Method of somatic embryogenesis of cassava and rapid propagation of regenerated plant |
| CN103122340A (en) * | 2011-11-18 | 2013-05-29 | 中国科学院上海生命科学研究院 | Method for improving agronomic characters of cassava and application of method |
| CN102783415A (en) * | 2012-07-12 | 2012-11-21 | 中国热带农业科学院热带作物品种资源研究所 | Method for conservation in vitro of cassava germplasm resources with stability and high efficiency |
Non-Patent Citations (6)
| Title |
|---|
| N.K.KONAN ET AL.: "An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems", 《PLANT CELL REPORTS》 * |
| PENG ZHANG ET AL.: "Transfer and expression of an artificial storage protein (ASP1) gene in cassava (Manihot esculenta Crantz)", 《TRANSGENIC RESEARCH》 * |
| PENG ZHANG ET AL.: "Transfer and expression of an artificial storage protein (ASP1) gene in cassava (Manihot esculenta Crantz)", 《TRANSGENIC RESEARCH》, vol. 12, 31 December 2003 (2003-12-31), pages 243 - 250, XP009011526, DOI: doi:10.1023/A:1022918925882 * |
| 曾文丹等: "优良木薯品种新选048离体快繁技术", 《南方农业学报》 * |
| 莫饶等: "木薯离体培养的研究", 《华南热带农业大学学报》 * |
| 黎萍等: "多效唑在木薯组织培养中的应用研究", 《黑龙江农业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258961A (en) * | 2016-08-11 | 2017-01-04 | 广西作物遗传改良生物技术重点开放实验室 | A kind of method of liquid culture Maninot esculenta crantz. Potato microtuber |
| CN110558229A (en) * | 2019-09-30 | 2019-12-13 | 海南大学 | Seedling hardening method for remarkably improving transplanting survival rate of cassava tissue culture seedlings |
| CN113142056A (en) * | 2021-04-30 | 2021-07-23 | 中国农业科学院油料作物研究所 | Flax rooting culture medium and rooting promoting method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104094852B (en) | 2016-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104686332A (en) | Method for establishing tissue culture regeneration system of camellia sinensis L. | |
| CN101946602A (en) | Method for culturing and transplanting seedling of cassava | |
| CN107087502B (en) | Method for cultivating double-sprout-tiller grafted seedlings of tomatoes | |
| CN102498844A (en) | High-yield sweet potato cultivation method | |
| CN104094846A (en) | Method for rooting and thickening of fructus momordicae tissue culture seedlings | |
| CN101720670A (en) | Rapid breeding method for pinellia tuber tissue culture | |
| CN102577969A (en) | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 | |
| CN102487816A (en) | Wheat double haploid production method by wheat-and-maize distant hybridization | |
| Abbaszadeh et al. | An effective nutrient media for asymbiotic seed germination and in vitro seedling development of phalaenopsis ‘Bahia Blanca’ | |
| CN104186321B (en) | The root culture method of pears test-tube plantlet and substratum | |
| CN107211891B (en) | Konjak callus direct seeding and rapid propagation technology | |
| CN108142283B (en) | Tissue culture rapid propagation method of Acer catalpa Maxim | |
| CN104094852B (en) | A kind of method of cassava plantlet in vitro strengthening seedling and rooting | |
| CN101946706B (en) | Method for regeneration system establishment of sweet sorghum young ear callus | |
| CN104686116A (en) | Topping and cutting propagation method for energy crop--silvergrass | |
| CN105028084A (en) | High-efficiency seedling raising technology of pear tree | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN112425506A (en) | Tissue culture rapid propagation method of artemisia anomala | |
| CN101161058A (en) | A breeding method of root cancer resist cherry rootstock sprout as well as group culturation rapid propagating technology | |
| CN105104142A (en) | Eugenia uniflora water culture cutting propagation method | |
| CN102823499B (en) | Factorized breeding method of blueberry tissue-cultured seedlings | |
| CN101518205B (en) | Sundew asepsis seeding reproduction method | |
| CN113994866A (en) | Method for improving rice seedling raising soil by artificial humus | |
| CN100452959C (en) | Raising seedling method of cutting open sweet potato to half | |
| CN105706840A (en) | Method for improving cutting propagation survival rate of clerodendrum trichotomum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221223 Address after: 530007, 174 East University Road, the Guangxi Zhuang Autonomous Region, Nanning Patentee after: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Address before: 530007, 174 East University Road, the Guangxi Zhuang Autonomous Region, Nanning Patentee before: Institute of Economic Crops, Guangxi Academy of Agricultural Sciences |
|
| TR01 | Transfer of patent right | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20141015 Assignee: Fuchuan Taro Union Planting and Breeding Professional Cooperative Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046561 Denomination of invention: A Method for Strong Rooting of Cassava Tissue Culture Seedlings Granted publication date: 20160406 License type: Common License Record date: 20231108 Application publication date: 20141015 Assignee: Guangxi Fuchuan Dexin Ecological Agriculture Development Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046559 Denomination of invention: A Method for Strong Rooting of Cassava Tissue Culture Seedlings Granted publication date: 20160406 License type: Common License Record date: 20231108 Application publication date: 20141015 Assignee: Guilin Guanyang SHANGNONG Agricultural Development Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046558 Denomination of invention: A Method for Strong Rooting of Cassava Tissue Culture Seedlings Granted publication date: 20160406 License type: Common License Record date: 20231108 Application publication date: 20141015 Assignee: Guangxi Shunteng Agricultural Products Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046557 Denomination of invention: A Method for Strong Rooting of Cassava Tissue Culture Seedlings Granted publication date: 20160406 License type: Common License Record date: 20231108 Application publication date: 20141015 Assignee: Guangxi Hezhou Funong Ecological Agriculture Co.,Ltd. Assignor: GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046556 Denomination of invention: A Method for Strong Rooting of Cassava Tissue Culture Seedlings Granted publication date: 20160406 License type: Common License Record date: 20231108 |
|
| EE01 | Entry into force of recordation of patent licensing contract |